trabajo de clostridium botulinum

7/23/2019 Trabajo de Clostridium Botulinum

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UNIVERSIDAD NACIONAL DE SAN ANTONIO ABAD DEL CUSCOFACULTAD DE CIENCIAS AGRARIAS TROPICALES

CARRERA PROFESIONAL DE INGENIERIA EN INDUSTRIAS ALIMENTARIAS

ASIGNATURA: MICROBIOLOGIA GENERAL

DOCENTE: BLG.WILBERT QUISPE ZUÑIGA

TEMA: CLOSTRIDIUM BOTULINUM



PRESENTADO POR:

JUAN JOSE HUARCA SUTTA

PAUL ZAMALLOA VARA

• JUNIOR GALVEZ VENEGAS

QUILLABAMBA, NOVIEMBRE 2013

INTRODUCCIÓN

L! "#$%&! G'#( )!%*%+! '(#-'! - !)'#! !/ &#! !)$%! - /'!

B#$%&&! C&!*'%-%(. E!*! "#$%&! !/ $!()&%*#! -"%- # 4 '(#/

!)'#!, )-/ !"'+%+%' / & #("%/* )' ($5! #6!. E& /' B#$%&&! !

#'"%, / *#/* 4 &! $&!*'%-%! !/ #/#'"%! "&%#-!.

L! $&!*'%-%! !/ "#$%&! '#/-! (7+%&!, '#()!%*%+! #/#'"%!, ($5!

-!$()// )'*8/#!, '(#/ *9%/#!. L#! !)'#! - &! $&!*'%-%! !/, )' &

/'#&, (#'! 4 & -%(*' - &! "#$%&! 4 &#! '(#/. E/ &#! -%+'!#!

!)$%! &#! !)'#! ! /$/*'#/ $&$#-#! $#*'%#&, !"*'(%/#& *'(%/#&.

S/ (7+%&! )'!/*#/ &#&! )'%*'%$!.

L! $&!*'%-%! $()#'*/ #/*8/! )' *#("%/ )!/ #/*8/! !)$8%$!

!&"&! 4 )'(%*/ #')#'&! )' (-% - )'"#! - )'$%)%*#$%7/.

S 5"%*#* /#*'#& ! & !& & %/*!*%/ - &! #/%(#&! & 5("', -/-

+%+/ $( !#)'7%*!. E/*' &! )#*7/! ! /$/*'#/ &! $#!#/*! -&"*&%!(, **#/! &# #/'/# #!!#.

L! $&!*'%-%! $#!#/ +#'%#! /'(-#-! '#+! (-%#-#! )' *79%/#!:

Clostridium tetani , **#/!. Clostridium botulinum, "*&%!(.



Clostridium difficile, $&%*%! !-(("'#/!#. Clostridium perfingens, #/'/# #!!#.

DE;INICI<N:

E! /# "#$*'%# G'#( )!%*%+#, #/#'"%#, 4 ! /$/*'# )' & /'#& /

&# *%' '# ! ) '-$* '# - &#

*9%/# "*&8/%$#, & #/* $#!#&

-& "*&%!(. E!*! (%$''#/%!

(! 4 * %// '(# - +#'% &&#!

! -!#''&&#/ (='

/ $/- %$ %/! - )$

9 8 / . L#! "#$ * ' % #! '( #/

!) ' # ! 4 & ! ) ' ( % * /

!"'+%+%' / / !*#- &#*/* 5#!*#

!' 9)!*#! # $/-%$%/! 4 )-#/ !!*/' ! $'$%(%/*. L# !)'# !+#&#-# !"*'(%/#& -'(#/*. E! (7+%& )' &#&! )'%*'%$!, /

)'-$ $)!&# ! )'*&8*%$ &%)&8*%$

CARACTERISTICAS GENERALES:

o B#$%& '#()!%*%+!.

http://es.wikipedia.org/wiki/Bacteria

http://es.wikipedia.org/wiki/Bacteria_Gram_positiva

http://es.wikipedia.org/wiki/Espora

http://es.wikipedia.org/wiki/Flagelo

http://es.wikipedia.org/wiki/Bacteria_Gram_positiva

http://es.wikipedia.org/wiki/Espora

http://es.wikipedia.org/wiki/Flagelo

http://es.wikipedia.org/wiki/Bacteria



o A/#'"% !*'%$*.

o E!)'&#- >/ )!%$%7/ !"*'(%/#&?.

o P'8*'%$.

o S%/ $)!&#.

o S%/ )%&%!, /% %("'%#!.

o S /$/*'# / & !& +%'/ $&*%+#-.

o H#"%*# / & %/*!*%/ - #&/! #/%(#&! !%&+!*'! -(!*%$!.

o P'-$ &# *9%/# /#*'#& (! )*/* 4 ! $/$. P'-$ &# ('* /'(# ( ')%-#.

CARACTERISTICAS ESPEC@;ICAS

P'-$ &#! *9%/#! (! )*/*! $/$%-#!. S 5#/ %-/*%%$#- $5 +#'%-#-!

- #/*8/! -%'/*! - &# *9%/# AH.

• L! *%)! A, B E !/ &! 4 ! '&#$%/#/ $/ /'(-#-! / &

5(#/: !'*%)! A B #!$%#-! $/ $#'/ E #!$%#-! $/ )!$#-.• T%) C )'-$ & $&& "&#/- / &! )&&!.• T%) D $#!# "*&%!( / & #/#-.

L# -!%! ('*#& / & 5("' - &# *9%/# ! %/'%' # 12 . L#! *9%/#! !/

-!*'%-#! )' $#&/*#(%/* -'#/* 20 (%/*! # 100C

CONDICIONES DE SUPERVIVENCIA



EPIDEMIOLOGIA:

• U"%$!: !&, ##!.• P-/ $/*#(%/#' #&%(/*! >+*#&!?, 5'%-#! $&/%#' & *'#$*

-%!*%+.• I/!*# - #&%(/*!. N ! %/+#!'.• P'-$ &# 9*9%/# (! )*/* 4 ! $/$.• L# *9%/# ! /# /'*9%/#.• R#'# / 5(#/! )' $(/ / #/%(#&!.• ;'(# (! $(/ - "*&%!( ! & "*&%!( *'#/!(%*%- )' #&%(/*!.

CLOSTRIDIUM BOTULINUM: TOINAS

T'(&"%&!. P-/ -!#''&&#'! *9%-!.

E9%!*/ F *%)! #/*%/%$#(/* -%'/*! >AG?. E/'(-#- 5(#/# #!$%#-# #

*%)! A, B, E >;?.

N'*9%/#:



• A$*# # /%+& -& !%!*(# /'+%!

)'%'%$ >)&#$# /'(!$&#'

!%!*(# #*7/(?.• A)#'%$%7/ - )#'&%!%! &$%-# - &#

(!$&#*'# !4&*%$# #&&

)#'#!%()*%$• B&4# &# &%"'#$%7/ -&

/'*'#/!(%!' #$*%&$&%/#.

>A$*%&$&%/!*'#!#?

PATOGENIA DE LA EN;ERMEDAD

1. Causado por alime!os:

E! &# (! '#+ $/*%/ /# )*/* /'*9%/# '(#-# -'#/* &

$'$%(%/* "#$*'%#/. L# *9%/# )- -!*'%'! )' $#&/*#(%/* # 0

C -'#/* 10 (%/*!. L# %/$%-/$%# - &# /'(-#- ! "#=#, )' !

$/!%-'#-# - &+#-# *#!# - ('*#&%-#- !% / ! -%#/!*%$# ! *'#*#

')%-#(/*. A)'9%(#-#(/* /*' & & 10 - &#! )'!/#! $/

"*&%!( )' +8# #&%(/*%$%# ('/. C/ '$/$%#, ! -" # #&%(/*!/&#*#-! / $#!#, )'$!#-! - (#/'# %/#)')%#-#. U/# )'!/# -"

%/'%' #&%(/*! $/*#(%/#-! 4 / 5##/ !%- $$%-! '$#&/*#-!

#)')%#-#(/* -!)! - 4 &# *9%/# 5## !%- )'-$%-# )' &#!

"#$*'%#!

2. I"a!il:

E! $#!#- )' %/!*%7/ - !)'#! - C&!*'%-%( "*&%/( 4

$&/%#/ )'-$ &# *9%/# / & *'#$* %/*!*%/#& - &! /%6!. L# %/!*#

- (%& !* '&#$%/#-# $/ !*# /'(-#-, / ! -" #&%(/*#' #

%/#/*! (/'! - 12 (!! - -#- $/ (%& #&(8"#' - (#8. E/ &

"*&%!( %/#/*%&, & "" -" %/'%' !)'#! "#$*'%#/#! & )'-$%'

&# *9%/# / ! !%!*(# #!*'%/*!*%/#&



3. Causado por #eridas:

O$'' $#/- /# 5'%-# ! 5# $/*#(%/#- $/ *%''# #'/# '!#

%/$'!*#-# / &# )%& &# 5'%-# ! #%!&#-# -& #%' '!$ $'' $#/-

! %/*'-$/ !)'#! -& "*&%!( / /# 5'%-#. L# 5'%-# -"

-!"'%-#'! >'*%'#' &# !$%-#-, &! "=*! 9*'#6!, & *=%- &!%/#-

)#'# +%*#' &# %/$$%7/ #$%&%*#' &# $%$#*'%#$%7/?. E! &# '(# (! '#'#

$'' !%(%&#' #& **#/.

. Forma si $lasi"i$a$i%:

S *'#*# - /# '(# -& "*&%!( -& &#$*#/* 4 #)#'$ / #-&*!

!* #!$%#- # #/(#&8#! / & *'#$* -%!*%+.

HABITAT K ALIMENTOS IMPLICADOS:

S! !)'#! !*/ -%!*'%"%-#! / &# /#*'#&# *#/* / &! !&! - $&*%+

$( &! - '#/ +*#$%7/. E/ !-%(/*! - &#! $#8-#! - ##, / &! &#!

&#! ##! $!*'#!, / & *'#$* %/*!*%/#& - &! )$! (#(8'!, #!8 $( /

&#! "'#/4%#! / &#! +8!$'#! - &! $'!*$! *'! (#'%!$!. T#("%/ !

/$/*'# / & )&+.

L#! $#'/ )'-$*! $'/%$!, !(*%-! # )'$!! - $/!'+#$%7/

/&#*#- $#!'! $( )' =()& & =#(7/ #5(#-, (#' - $'-,

)#*! ("*%-! >& /("' - "*&%!( -'%+# -& &#*8/ "*&!

("*%-?. L! )$! *#("%/ ! )-/ $/*#(%/#' $/ C. botulinum, !)$%#&(/*

)' & *%) E, / & (-% #$*%$ - #48 4 &! )'-$*! #5(#-!

-'%+#-! -& )!$#- 5##/ !%- '!)/!#"&! - +#'%! "'*! -

"*&%!(. L# $/*#(%/#$%7/ -& !& ! / '%/ %()'*#/* - C. botulinum

9%!*/* / &! #&%(/*! $/!*%* / '%/ #& 4 !*/

%/+%*#"&(/* 9)!*#! &#! 5'*#&%#!, - (- !)$%#& &! $&*%+! -

'#8$! "&"!, $( )' =()& )&#*! )')#'#-! - /!#&#-#! -



)#*#*#! $$%-#! >)#'$%#&(/*?, /!#&#-#! - 5'*#&%#! )' /+#!#-#!,

*$... L# *9%/# ! %/!*#"& # *()'#*'#! 4 !)'#/ &! 30 C # )H

!)'%' # F # / )H (/' - . / 5# /% $'$%(%/* /% )'-$$%7/ -

&# *9%/#. P!* 4 &! #&%(/*! /&#*#-! )$ $%-! )-/!#*%!#$' *-! &! $'%*'%! #/*'%'!, )#'# #'#/*%#' ! %/$%-#-, / &#

%/-!*'%# -& /&#*#- 5# !%- /$!#'% %()&#/*#' '%'!#! (-%-#! -

$/*'& -& *'#*#(%/*. S%/ ("#', $#/- &! #&%(/*! /&#*#-! !

)')#'#/ $( #$*%+%-#- -(!*%$# / )46# !$#&# >5'*#&%#!

("*&&#-#! /&#*#-#!?, &# (#' +#'%#"%&%-#- & $/*'& (/! '%'!

-& *'#*#(%/* #& 4 ! !(*/ !/ +%-/*(/* '%/ - )'"&(#!

!#/%*#'%! - %()'*#/$%#.

MEDIDAS DE CONTROL ALIMENTARIAS:

1. L# *9%/# ! *'(&"%&, )' & 4 ! )- -!*'%' #& $#&/*#' &# $(%-#

-'#/* 10 (%/*! # 0 C100C2. A!'#'! 4 & *'#*#(%/* *'(%$ - &! #&%(/*! ("*&&#-!

/&#*#-! >)H.? ! !%$%/* )#'# -!*'%' &#! !)'#! (! *'(

'!%!*/*! - C. botulinum.3. U*%&%#' )#'# &# ''%'#$%7/ - &#! &#*#! )'$!#-#! ## $&'#-# & (!

&%()%# )!%"&. C#/- ! *%&%$ #%' '8 #!'#'! - 4 &#

$/*#(%/#$%7/ $'#-# # )#'*%' - &#! (#*'%#! )'%(#!, -& 4%) !$% #

$/!$/$%# - &# (#/%)&#$%7/ ! +%*# -'#/* &# ''%'#$%7/.4. C#/- ! ()&/ *'#*#(%/*! *'(%$! !#+! #!'#'! 4 !

#6#-/ $()!*! %/5%"%-'! #-$#-! 4 & )H ! &

!%$%/*(/* "#= $( )#'# )'+/%' & $'$%(%/* - C. botulinum.5. C()'"#' 4 & *'#*#(%/* *'(%$ - &! #&%(/*! )'$!#-!

!(%)'$!#-!, /+#!#-! #& +#$8, ! & #-$#- 4 / !

#&(#$/#/ # *()'#*'# (#' - 3C.6. N/$# -" )'"#'! / #&%(/* !!)$5!, # / !' 4 ! )'$#&%/*

# 100 C.F. R$5##' $/!'+#! $/ /+#! #"("#- 9%-#-. A)&%$#' #&*#!

*()'#*'#! - $$%/#-.



LA TOIIN;ECCI<N ALIMENTARIA

1. S&!omas: I/$&/ +%!%7/ "''!# -"&, -"%&%-#- /'#&, '&=!

)"'!, -%%$&*#- )#'# *'##', '!)%'#' 5#"&#', +'*%!, )#'&%!%! &$%-# , #

+$!, &# ('* )' %/!%$%/$%# '!)%'#*'%# "!*'$$%7/ - &# /*'#-# -

#%' / &# *'4#. E/ $#/* # &! !8/*(#! #!*'%/*!*%/#&! !/: -&'

#"-(%/#&, -%#''# $/!*%7/.

2. Periodo de i$u'a$i%: D 12 # 3 5'#!, )' )- 9*/-'!

5#!*# -8#! / #&/! $#!!.

3. Tra!amie!o: S /$!%*# #*/$%7/ 5!)%*#&#'%#. S #-(%/%!*'# /#

#/*%*9%/# )&%+#&/* - '%/ 4%/ / $%'*! $#!! - "*&%!(

)'+$#- )' #&%(/*!, #/4 / / $#!! - "*&%!( %/#/*%&. P-

!' /$!#'% */' 4 %/-$%' #& +7(%* #)&%$#' / /(# )#'# '*%'#' &!

#&%(/*! $/*#(%/#-!. T#("%/ ! #-(%/%!*'# )/%$%&%/# G.

N 9%!* & $/*#% /*' )'!/#!. E! - !+'%-#- +#'%#"& >- &+ #

('*#&?, $#/* #/*! ! )'!/*#/ &! !8/*(#! (! '#+ !. E/ $#! -

$'#$%7/, &# '$)'#$%7/ )- -'#' - +#'%#! !(#/#! # (!!. L#!

)'!/#! 4 5#/ !'%- "*&%!( )-/ !'%' #*%# -%%$&*#-

'!)%'#*'%# -'#/* #6!.

. Dia(%s!i$o: L# '(# (! $*%+# -%'$*# - $/%'(#' & -%#/7!*%$

$&8/%$ - !*# /'(-#- / & &#"'#*'% ! -(!*'#/- &# )'!/$%#

- &# *9%/# / & !' / &#! 5$! $#&! - &! )#$%/*! / &!

#&%(/*! $/!(%-! )' &! (%!(!. A$*#&(/* & (*- (! !/!%"&

- (#' ! )#'# &# -*$$%7/ - &# *9%/# ! # *'#+! - &#

/*'#&%#$%7/ - / '-'.



DETERMINACION DEL CLOSTRIDIUM BOTULINUM EN

ALIMENTOS

• C&*%+ / A#' - (# - 5+.

• C&*%+ / $#&- - $#'/ -

C&!*'%-%(

L# $#)# - #$%* (%/'#& !*'%& )'(%* 4

&# !)'%$% -& $#&- $/ $are molida de

$ora)% de *a$a 4- **#&(/* $"%'*#

)#'# %()-%' &# -%!%7/ - O2 #& (-%. A/*!

- %/$&#' ! $/+/%/* 5'+%' & (-%

-'#/* 10 (%/ )#'# &%"'#' & O2.

AISLAMIENTO E IDENTIFICACIÓN DE CLOSTRIDIUM BOTULINUM PRODUCTORA

DE TOXINA

MATERIALES.

• M-%! - $&*%+.• L#*#! - #*/ #"("#-#!, $5#/$#-#! #"&&#-#!.• R#*/! - &#"'#*'%.• T'%*'#-' ($/%$.• B#6 (#'8#.• T"! - -%&$%7/ $/ *#)# '!$#.• M$5' "/!/.• P%)*#!.• ;'#!$! !*'%&%#-!.• G'#-%&&#!.• C/*'%#.• A=#! 5%)-'(%$#!.



PROCEDIMIENTO.

P'"# 9)&'#*'%# - )'!/$%# - &# *9%/#

T'%*'#' 5(/%#' & #&%(/* >(!*'#? P/' / & *" - -%&$%7/ - *#)# '!$# (!*'# # %#& #& )! - &#

!&$%7/ !#&%/# # *%&%#' >-%&$%7/ 11?. C/*'%#' & 5(/%#- # #&*# +&$%-#-, )''/*(/* /

$/*'%# ''%'#-# >! */-'/ 2 *"!, !# -! (!*'#!?. C(! / - ! *"! $/*'%#-! & !(*(! # "#6 M#'8# )'

10 (%/. >## 5'+%-#?. D& $/*/%-, %/$*#(! 0. (&. A& '#*7/ - )'"#, !* $/ #=# -

*"'$&%/# >N 2? +8# %/*'#)'%*/#&. D& *' *" $/*'%#- ! & %/$*#'# # *' '#*7/ - )'"# - &#

(%!(# $#/*%-#- - %/$&. S '$(%/-# 4 #/*! - %/$*#'& & !"'/#-#/* # &! '#*/! ! &

-" %/$&#' &# #/*%*9%/# O"!'+#' )' -8#! L# )!%*%+%-#- - &# )'"# ! $()'"# )' &# )#'&%!%! (*'#

)''!%+#, ! #"-(/ $/*'#8-, '!)%'#$%7/ -%%$&*!#, #''#!*' - &#!)#*#!, -"%&%-#- - &# (!*'#.

L# )'!/$%# - &# *9%/# E $#!# /# ($5 (! ')%-# >/ (/! - 2?

AISLAMIENTO >/ #&%(/*!?:

• P#'# & #%!&#(%/* !#(! (-%! /'%4$%-! $( & - $#'/ $$%-#

& (-% *'%)*%$#!#&$!# >ATG?.• E/ *"! - -%&$%7/ $/ *#)# '!$# $&$#(! 10 (& -& (-% # !#' >!*

!*# )'+%#(/* $#&/*#-, )' & *#/* !(%&84%-?.• P'+%#(/* / $#-# *" 5(! #'#- / )$ - &# (!*'# &

#'#'(! / $#-# *" & ATG !(%&84%- $#&/*#- )'+%#(/*.• L !(*(! &! -! *"! # *()'#*'#! -%'/*!• E& )'%(' *" / ! $#&%/*#• E& !/- *" & $#&/*#(! / "#6 M#'8# # 0 C )' 10 (%/.• E& *'$' *" & $#&/*#(! # 0 C )' 30 (%/.• L %/$"#(! &! *"! )' 3 # -8#! / =#''# - B' W'

>#/#'"%!%!?• S )-' "!'+#' >!% &# (!*'# ! )!%*%+#? &# )'!/$%# - #!

'!4"'#=#(%/* -& (-%.

trabajo de clostridium botulinum

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