artificial insemination by allah dad khan
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- 1. Updated:07/22/15
- 2. But ThisNot This
- 3. Artificial Insemination is older than everyone in this rooms ages combined. Documents from around 1322 A.D. state that an Arab chief wanted to mate his mare to a stallion owned by his rival. So he preformed an amateur version of the artificial insemination we know today. Then in 1780, Spallanzani successfully bred two dogs with the use of A.I. Leading to him being named the inventor of artificial insemination. The mass breeding of cattle however didnt come until later, in 1931. By an A.I. Cooperation in Denmark.
- 4. Liquid Nitrogen tank Long gloves Insemination rod Paper towels Straw cutter Sheath Semen straw Warm water bath Thermometer Rubbing alcohol Clock Record books
- 5. Genetic improvement of livestock Disease control mechanism Possible to increase fertility Decrease breeding expense
- 6. Genetic Improvement Wide spread availability of genetically superior sires Disease Control Injured studs available More valid stud proofs Reduced danger from studs Cost - relatively cheap
- 7. Estrus detection must be good Handle and care for semen Record Keeping Time involved - Restraining and inseminating cow Training required to handle semen and breed cow
- 8. Semen Collection Sexual arousal using Sight, sound, smell, touch Best mount - Live Alternative mount - dummies available for: Stallions Bulls
- 9. Updated:07/22/15 Collection on a Live Mount
- 10. Electro-ejaculation Useful if male wont or cant mount Get urine often Poorer quality ejaculate Used in rams and beef bulls Massage Method Stimulate by rectal massage Seminal vesicles Vas deferens
- 11. Artificial Vagina Temperature Bull, stallion, ram at 45C Pressure Friction Lubrication essential
- 12. Extenders include: Nutrients Glucose, fructose Cold shock prevention Buffer Milk, skim-milk, egg yolk Citrate, Tris The buffer component Antibiotics Inhibit bacterial growth Increase volume Cryoprotectant glycerol
- 13. After collected and extended, semen is put into a semen straw. That straw is placed into a Liquid Nitrogen Tank. Each tank contains a Cane, which consists of Goblets containing the semen straws themselves. Make sure that the correct procedure for freezing semen in a liquid nitrogen is followed.
- 14. Use the procedures recommended by the semen supplier!!! 35C water for 30 - 60 seconds Ice water for 3 minutes Pocket thaw Be very careful when removing straw from Nitrogen Tank Thawing
- 15. Loading the AI Rod Using a straw cutter, the end is cut off of the straw of semen.
- 16. Locating the Cervix The technician should insert one hand into the cows rectum to locate the reproductive tract and cervix.
- 17. Cleaning the Vulva The skin around the vagina (the vulva) should be cleaned with paper towels to avoid contaminating the cow when
- 18. Time of insemination Cow - 12 hours after observed in standing heat (AM - PM rule) Sheep -12 to 18 hours after first seen in estrus Horses -Every second day beginning on day 3 of heat Insemination Protocol Rectal/ Vaginal Vaginal Once you have collected the semen and detected estrus of the female, you can begin to inseminate.
- 19. Pipette with syringe Lube Semen Straw Gloves (Arms Length)
- 20. Begin by restraining the animal. This will protect the animal and the technician during the procedure. STEP 1:
- 21. The inseminator places their arm into the females rectum, using plenty of lubrication, and removes all feces. This will allow the inseminator to palpate for the cervix more easily. STEP 2:
- 22. Once the inseminator has located the cervix they will hold it in their hand. Using their free hand the inseminator will obtain the syringe with a plastic sheath (pipette) already prepared with a straw of semen. STEP 3 & 4:
- 23. Carefully inserting the syringe with semen, being sure not to contaminate it with feces or other contaminates, the inseminator will insert the syringe into the females vagina. They will continue to pass the syringe until they can pass it through to the end of the cervix. STEP 5:
- 24. Once the end of the cervix is reached, the plunger on the syringe can be depressed allowing the semen to be deposited directly into the uterus of the female. The Sperm will then hopefully reach and fertilize the egg resulting in conception, and eventually a fetus. STEP 6:
- 25. If all goes well, the female animal becomes pregnant and carries the baby to term. Then once the baby is born it will be the result of a successful Artificial Insemination procedure.
- 26. Conclusion After AI is completed, normal fertilization will take place and a calf should be born in approximately 283 days.
- 27. Updated:07/22/15
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