this training is designed to familiarize you with all the ... · agilent pams autogc training...

This Training is designed to familiarize you with all the features of the Markes –Agilent AutoGC system. Orsat offers a configuration for fully automatic, unattended operation however this does not mean there is nothing for you to do.

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Agilent PAMS AutoGC Training 8/13/2018

Orsat has configured the Agilent Markes AutoGC System to facilitate field deployment and operations. We can provide the additional equipment to fully automate data collection in the field. You have been introduced to the basic system , the Unity XR and Kori with the CIA sample system along with the Agilent 7890 GC.

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There is additional ancillary equipment necessary to provide support gases for field operation. We also provide the Merlin MicroScience Dilution System used to generate calibration curves and nightly check standards.

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Orsat provides:1. A dilution system to generate calibration curves from a 1 ppmC calibration gas2. Software to generate sequences which will interface with our additional valving

installed and configured to introduce routine QC samples3. Configuration of all necessary analytical methods4. Complete configuration of all plumbing and support gas systemsWe can also provide:1. Deployment2. Field Qualification for 1 week to meet specification3. Training for operators and validatorsAlso available:Support contracts

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The ancillary equipment required for this system includes the necessary Air supply. 1. This generally includes an air compressor and the necessary purification systems

to supply dry air at < 1 ppm moisture for use with nafion driers if present and to blanket the Peltier thermo-electric coolers used to control the low trap temperature which would ice up if left in ambient air. In addition, the dual FID system requires ~ 800 mL/min of HC free air for operation. For our systems we require an additional 150 mL/min HC free air for the dilution of standards for calibration and check standards.

2. Additional gases required include ~80 mL/min of Hydrogen which can be either from cylinder gas or hydrogen generator.

3. And carrier Helium of ~ 10-50 mL/min. 4. Other equipment not included includes the necessary sample manifold and

blower for pulling in outside air and canisters and standards. 5. A Dilution system is used for dynamic dilution of calibration standards and

generation of daily blanks and check standards are recommended in lieu of frequent replacement of statically diluted check standards.

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1. The air system is the main support gas supply for the system. If it fails the system will not function so it is important to record flows and pressures and log any issues found on a daily or weekly basis. The compressor supplies a steady source of air for both the TOC gas generator and the zero air purifier.

2. The compressor is equipped with an autodrain which is important for mitigating moisture content in the compressed air. This should be tested at regular intervals to insure it is working properly.

3. The TOC gas generator supplies the dry air for the peliter coolers in both the Kori and Unity and is equipped with a flow meter and regulator down stream to monitor the flow and pressure being supplied to these instruments. The flow and pressure should be monitored and recorded in the log periodically to eliminate potential problems associated with leaks.

4. The compressor also supplies the air for the zero air purifier which supplies HC-free air to the FIDs. It also has a gauge installed which should be monitored regularly and any deterioration noted. The zero air purifier has a catalyst and filter which should be changed every year.

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Fuel gas for the FIDs can be supplied by cylinder or H2 generator. 1. Orsat recommends a H2 generator which eliminates the necessity of cylinder

changes and cylinder handling and demurrage. However, hydrogen generators have sufficient hysteresis that if there is a power outage it can take some time to re-build pressure resulting in an error on the gas chromatograph when the FID flames go out.

2. To reduce these issues the system has been supplied with an auto-restart feature and auto switching manifold connected to

3. a single backup cylinder of hydrogen which will automatically switch online should the hydrogen generator pressure drop below the setpoint. The hydrogen generator has been equipped with an auxiliary reservoir to reduce maintenance but water should be added regularly to keep the system from going low. High purity water is required for this system. The generator has a maintenance schedule for changeout of desiccant which is required every 6 months.

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Orsat also can supply an Uninterruptible Power Supply capable of sustaining the Markes equipment and Agilent GC for short intervals reducing the data loss associated with short power failures at the site.

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1. Our sites have a dilution system which allows the dilution of a 1 ppm standard for generating calibration curves as well as providing the automated daily Calibration Verification Standard and an analytical or system blank.

2. This dilution system is configured to be operated automatically from the Data System sequence and allows the automatic collection of the daily check standard as well as an analytical blank from the zero gas diluent. The system also allows for unattended automatic calibration curves to be run across 4 levels from nominally 1 ppb to 50 ppb. This system relies on the dilution of span gas by pressure through calibrated restrictors at low flow into a static zero gas flow which allows for minimal zero gas consumption and high stability.

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1. OpenLab EZChrom was chosen because it generates data files which are easily transported to another computer where they can be reviewed and reprocessed if necessary. It also can generate ASCII text output for ingestion into other systems.

2. We recommend the purchase of a second copy of the data system to be used offline at a remote location to reduce the risk of interfering with on-going monitoring activities to facilitate the review and reprocessing of any data.

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The EZChrom Data System 1. Identifies components using retention time windows and uses reference peaks to

accommodate routine diurnal drift2. Quantitates components using an average response factor based on Propane and

Benzene3. Allows for noise thresholds to reduce interference4. Uses a width sensitivity setting to minimize detection of spurious non-

chromatographic peaks.

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Key elements of the chromatographic method includes the Peaks and Groups table and Integration events.The Peaks and Groups table allows the entry of 1. The component name2. Retention time window3. And Response FactorThe Integration Event table allows the introduction of1. Width and threshold events as well as integration on/off

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The sampling is scheduled using a sequence which is generated using the 1. Merlin EZ Sequence program. This program generates a text file which when

loaded into the EZChrom sequence includes 2. routine QC samples, provides filenames which identify samples and the necessary

valving events to control the introduction of samples.

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Filenames generated by EZSequence allow sorting of data and easy identification of site and sample type as well as month, day and hour of the day the sample was collected.

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The overall data handling and validation of PAMS data can be tedious due to the large amount of data files. The system configuration includes an application which helps keep file handling organized. 1. There are basically 2 stages in the data handling for collection at the data site2. And movement to the site where validation and review can occurThis shows a schematic representation of the system hardware at the monitoring site and the process of sample collection and analysis. We supply an additional application called Mmove which will automatically move data for polling and archive data into daily zip files to keep data organized and reduce losses.

Ideally polling of data from the chromatographic system to a remote site will allow data to be uploaded to our cloud-based system MAX and evaluated using MAX Quicklooks and graphic capabilities. If necessary, chromatographic data can be reprocessed and reloaded into MAX to correct any integration or identification issues which might arise without the risk of interruption of the AutoGC collection of the data. The ability to flag data and generate AIRS files in MAX is currently under development. However MAX can generate crosstab files which can easily be uploaded to the AirNow DART validation tools.

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The AutoGC collects two data signals1. The PLOT Column where C2 to C6 NMHCs are separated, quantified and identified2. And the DB1 Boiling point column where the C6 – C10 NMHCs are separated and

identified.

This is a chromatogram of the PAMS standard diluted to nominally 1 ppbC by partial injection of the PAMS standard followed by humidified zero air.

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This is the CVS – Calibration Verification Standard or CCV – Continuing Calibration Verification (in PAMS TAD)1. PLOT Column – The CVS we use has 7 targets on the PLOT column across the

boiling point range including significant species like acetylene and butadiene which are of interest and potentially problematic

2. DB1 Column – which also has 7 targets across the boiling point range

The use of a smaller list of targets makes review faster but still allows evaluation of trap performance and facilitates robust peak identification by including retention time reference peaks.

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Method Blank – which is humidified diluent gas from the dilution system1. Plot Column2. Boiling point column

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Agilent instrument Configuration in EZChrom.

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1. Your system will have 2 instrument icons on the desktop, one for the online instrument and one for an offline instrument for data review and method modifications.

2. To review the instrument configuration Launch the online instrument.

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The Instrument Wizard automatically comes up – it is useful sometimes but can be disabled by removing the check box in 1. Show on instrument startup2. You can get the Instrument wizard any time using the wizard icon

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Once the instrument is up (it may show the last data it was opened with) 1. Choose File Open Method to open the 2. instrument method

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1. Choose the current instrument method which should be the only method in your method directory. Be patient it may take a minute for the method to open – it will display the Instrument setup once it has loaded.

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The instrument method setup will be displayed.1. You can maximize the window by closing the navigation screen by clicking on the

left arrows on thee Navigation bar.

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Here is the maximized instrument method. We will go through the instrument configuration. Starting with 1. Valves - There are only three relays listed as valves #1, 7 and 8 in the method. 2. Valve/relay #1 controls the dean’s switch valve and 3. Valve/relay #7 and #8 are connected to the Orsat MMSI interface and are used to

reset other valves in the dilution system to control the automatic introduction of QC samples.

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1. The Inlet refers to the SSL Front Inlet – The SSL is providing the helium carrier to the Markes Kori and Unity xr. The inlet pressure is nominally 38 psi and the septum purge will be between 0.3 to 1 mL/min.

2. The septum purge acts like an outlet split so the true outlet split is the split on the Unity method plus the septum purge.

3. The Inlet Mode is splitless so the purge flow is not used however the time of 999.99 min needs to be in place to inhibit this from coming on and gas saver should be set to off.

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The Columns section shows the column descriptions and their flows1. Column 1 is the PLOT column which is a 50 m HP-PLOT Al2O3 S 2. The setpoint should be 4.5 mL/min and the column set to3. Constant Flow – all other values will be calculated by the software.4. Post run set point should also be 4.5 mL/min

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Column 2 is a 50 m DB-1 column with an optimum flow of 3 mL/min1. Again set to constant flow2. And a post run flow of 3 mL/min

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The last section is actually not a column but the restrictor which attaches from the deans switch to the back detector.1. Its flow is set to match the flow of the DB-1 column automatically

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The Oven profile was developed to give the best separation for both columns. The starting temperature is 45C which should allow the GC to equilibrate in an ambient environment of 75F which is common in monitoring trailers.

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The Detector section describes the FID flows and temperatures1. The detectors both are set to 250C and will not light until they reach this

temperature in the event of a power failure or from startup.2. The Air and H2 flows are 350mL/min and 35 mL/min respectively 3. And the detectors require a make-up flow of 10-20 mL/min4. Carrier gas is set to constant makeup and fuel flow5. And No column compensation

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In the event section the on and off times for relays for Valves #1, 7 & 8 are listed.1. Valve #1 which controls the Deans switch, is turned on at the beginning of the run

and 2. Off at the desired cut-time which is defined as between 1-hexene and n-Hexane

on the DB-1 column.The other relay events are used to reset valves in the dilution system and MMSI sample interface.

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The Signals section describes which signal is on which channel. 1. Signal #1 is set to record the front FID and signal #2 the Back FID. Both should be

set to 5 Hz/0.04 min sampling rate. 2. Both signals should have checked boxes for zero and save.

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The configuration section of the method shows how the Agilent GC is actually configured.The Miscellaneous section shows the configured valve relays1. Valve #1 is the deans switch relay and2. Valve #7 & 8 are configured as other relays for use with the MMSI valve box.

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Under configuration, the columns section is where the column connections to their respective carrier supplies is setup.1. The PLOT column should show an inlet of Aux EPC1 which represents the

connection to the Deans switch and shows an outlet at the Front Detector2. The DB-1 column shows its inlet as the Front inlet which is the SSL – this is the

source even though the column is actually connected to the Markes Unity xr since the Front inlet is supplying the carrier to the Markes system. Its outlet is Aux EPC1 since it is connected to the Deans switch.

3. The third column is the restrictor which connects the deans switch to the Back Detector so it shows an inlet of Aux EPC1 and outlet of Back Detector.

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The modules section shows the gas selection for each of the flow control devices.Front inlet or SSL – heliumFID (both) makeup – heliumAux EPC1 (Deans switch) Helium

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In addition to the actual instrument parameters such as columns and oven profile, the EZChrom method also contains the parameters necessary for identification, quantitation and output of the NMHCs in the PAMS target list.

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1. Method parameters are assigned by which FID they apply to. 2. Each Signal/FID has associated with it Timed Integration events3. A Peak Table which shows all the targets being detected on that signal along with

their respective retention time windows and response factors among other details.

4. The Sequence is used to schedule the sequence of samples.

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1. Advanced settings are shown by accessing the drop-down Methods Advanced2. The data export tab allows the export of a running tab delimited file if desired of

the results. It is not implemented but can be if you have a need for this data output.

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1. The Files tab shows where user programs can be executed by the method for each run.

2. This is where the executable Mmove file is defined which moves and archives data after the end of each run.

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1. The final Advanced Reports tab shows the 2. template we have developed which generates the *.tx1 file we use to import data

into MAX.

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1. Each Column/FID signal has its own set of parameters for their respective chromatograms

2. The Integration Timed events includes events for width and threshold settings which control peak detection as well as integration on/off

3. The Peak table is where you will define peaks under the 4. Named Peaks tab.

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The second tab on the Peaks and Groups tables allows the configuration of Groups to be reported separately from the individual peaks. We have 2 groups – Targets and UnID.1. Targets are defined as named peaks 2. and the group definition is Add All.

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1. The UnID peaks group is defined as a Calibrated range2. In the Group Range Definition you show the start3. And stop time for the range where peaks are to be included 4. And check the calculate concentration for unnamed peaks in the group.

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In the Peaks and Groups tables you can customize what columns are shown by 1. right-clicking on the 2. column header and 3. choosing the columns to display.

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EZChrom has a number of display features to facilitate review of the chromatographic data. This is generally done in the offline instrument.

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In the offline instrument you can view completed data files.1. Using File Open Data you can choose the 2. data file you want to display.You may need to select View Display all data to open the chromatogram windows and use Window Tile horizontally to get chromatograms to fill the view. Note: whichever window is selected when you do the tile command will be on top.

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The open file menu you get from FileOpenData shows the files in the current directory by default. You can navigate to any folder such as archive\rts to get files which have been parsed out for reference.1. This menu allows you to select what information about the data you want to

browse, either a thumbnail of the chromatogram or the report header

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If choosing to browse thumbnails you can choose which signal you want to view.

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In the open file menu you can also specify what method to use with the data file, Current is the current method file you may already have openFrom results is the data file embedded in data files where multiple results may be savesOriginal acquisition is the original acquisition method and will also be embedded in the data file and most likely the only method embedded in the data file.

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To change the way the data is displayed you can right click on either chromatogram and 1. choose properties.

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1. The Data Graph Properties dialog box will pop up which allows you to configure how the chromatogram is scaled, as well as what information is displayed.

2. When you select Annotations

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The Trace Annotation Properties dialog shows the additional information which will be displayed on the chromatogram including things like retention time, name, concentration, area. 1. The lower section allows you to display the peak baselines, retention time

windows and undetected peaks where they should have been.

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Axis setup in the Data Graph Properties dialog allows you to change the scale of the chromatogram and what information is displayed on the chromatogram legend. Autoscale is default.1. The Left Y-Axis can be set to manual to achieve your desired scaling – TIP: use get

current axis limits as a starting point. Note this will be maintained for all subsequent data files.

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The Appearance tab in Data Graph Properties allows the selection of a number of parameters associated with the color and of lines, fonts, font colors associated with each trace and can be saved as a “scheme” for reuse.

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To compare your current data file to previous ones such as an RTS or multiple ambient samples, 1. Right-Click on the chromatogram and choose 2. add trace from other data.

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When the Open Data File window pops up you can select from data in the current directory or change to the archive directory if you want to use older QC files1. Select the preview window to preview the file or details2. You can add multiple files here also

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After selecting the files you want the Data Graph Properties dialogue allows you to configure how the additional files should be displayed1. There are multiple tabs – the Trace Setup tab allows you to chose the signal you

want to compare, 2. either Front (PLOT) or Back (DB1)

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1. You can also choose how the additional traces will be scaled – to scale all traces the same choose which trace to scale to – when comparing an ambient to the RTS you may want to use normalized.

2. Note the multiple chromatograms will be overlaid – this may make review difficult

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1. In the Trace Setup tab you can then put in Y-offset values for chromatograms to allow them to be staggered.

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Once these setting have been applied zooming using the click and drag will apply to all chromatograms allowing inspection of retention time alignment.

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Right Click and choose annotations allows the configuration of annotations for each of the chromatograms to reduce unnecessary “clutter”

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Quantitation of the PAMS targets requires calibration. Calibration is required anytime the system response changes. To insure the system is working correctly the calibration also supplies information on the system linearity across the monitoring range. This requires the analysis of known standards for at least 3 concentrations and is done here using the Merlin MicroScience Dilution system. Since each run is 1 hour doing multipoint calibrations is time consuming however the MMSD-MPV allows the calibration to be scheduled within the sequence and run after hours.

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1. The typical calibration strategy used for the PAMS continuous AutoGC systems as outlined in the PAMS Technical Assistance Document is for carbon based response factors.

2. Carbon Based Response factors only requires certified standards for the reference components – propane and benzene.

3. It uses the average response factor across its range and 4. is easily applied and confirmed for all targets. 5. This is possible only with GC-FID systems.6. The response factor for our method is defined as peak area divided by the

theoretical concentration

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The Dilution system is an orifice based dynamic dilution system which uses 1. fixed restrictors which are calibrated based on the pressure input. 2. A hydrocarbon free air source is used for the zero gas diluent which is humidified using a 3. nafion permeation device which allows humidification of the zero gas internally. This zero gas is then used either to supply the daily blank or to dilute the daily check sample as well as generate multiple dilutions for the calibration curve.1. The Zero gas is set to 40 psig which generates approximately 150 mL/min of humidified

air which is flowing continuously out the 2. Excess port on the rear of the system3. Through EZChrom Valve 1 can be actuated to allow the zero gas to be directed to the4. OUT port which is connected to the sample line to the Markes instrument for collection

of the nightly blank sample5. To run a diluted check sample Valve 2 is actuated which directs the span through the 6. selector to the chosen restrictor and then to the 7. Blend point where it is diluted with zero gas and ultimately out to the sample manifoldThe span gas is supplied from a 15L summa can at 1 ppm which is limited to 30 psi max. To maximize sample the span pressure is 1. set to 5 psig. This flow is very low so the canister will last a long time. However to

facilitate the clearing of the span flow path 2. a fixed vent is used to allow the span transducer and flowpath to depressurize making

the total span usage ~ 3 mL/min.

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Although samples are generally run automatically from within EZChrom, the system can be used manually by using the front panel controls.1. Zero gas which is supplied from the Zero Air purifier is connected at the rear of

the instrument2. The blender output is connected into the sample line from the rear 3. The zero gas will flow from the sample excess port on the rear of the blender

when it is not directed to the sample line using Valve 14. The zero gas pressure is set on the front of the blender using the zero pressure

regulator5. And the span pressure is set on the front as well however you must toggle V2 on

to see the setpoint6. Toggle switches for Valve 1 which directs the blender output to the sample line

and Valve 2 which turns the span gas on or off are located here.7. Generally the blender selector should be in position 1 for setting pressures and is

also the recommended position for the nightly check sample however this can be changed if a different level is desired for the nightly check sample.

8. The selector is actuated using step and home command on the selector control

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The Dilution spreadsheet is designed to be a tool for the operator to not only execute the necessary calibration curve but also the track the instrument performance on daily and weekly quality control samples. The spreadsheet is configured with multiple worksheets which are described in the Blender Information worksheet.

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The TAD Guidance recommends the curve should span the range of 1 – 25 ppmC for propane and benzene and meet these criteria. The Curve_Response Factorsworksheet facilitates the evaluation of the resulting curve as well as providing documentation of the samples used to generate the response factors used in the EZChrom methods.1. The spreadsheet calculates the dilution based on the pressure setpoints for the

Zero and Span pressures – 40 psi zero gas and 5 psi span gas results in zero gas consumption of approximately 150 mL/min and span consumption of less than 3 mL/min.

2. The calculated ratios are used to determine the 3. Theoretical concentrations based on the original standard C of A concentrations

entered into the Nightly CVS Standard Worksheet4. These concentrations are linked to the Nightly CVS Worksheet5. The worksheet allows operators to enter the filename of the data files used for

the curve 6. And the area counts for each level and then 7. Calculates the response factors for each level8. As well as the average response factor and the %RSD9. Additional calculations are automatically generated based on TAD guidance for

the r2, y-intercept and % of theoretical for each level.

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The TAD Guidance recommends the curve should span the range of 1 – 25 ppmC for propane and benzene – the system as it is setup now was run with a 1 ppmv starting standard. This is a duplication of the previous spreadsheet 1. showing the theoretical concentrations which can be accomplished with this

same hardware by using a standard of 1 ppmC instead of 1 ppmV

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Once the response factors for the curve have been determined they must be entered into the method. In the offline instrument, 1. Use File Open Method to open the running method

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1. From the Method drop down menu choose Peaks/Groups

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For each signal you will enter the RF for all Named Peaks and Groups1. On the Named Peaks Tab you will enter the average RF from the calibration curve2. in the column for Manual RF3. Right click on the first cell after you have entered the value and you will get a

menu which will allow you to Fill Down

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1. On the Groups Tab you will need to 2. enter the RF for the UnID peaks on the Groups Tab as well

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You will have to choose the Back signal and do the same operation1. Named Peaks2. Enter value and right click3. Fill down

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1. Select the Groups Tab 2. To display the Group table and you will need to 3. enter the RF for the UnID peaks on the Groups Tab as well4. Be sure to save your method

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Additional worksheets are provided to allow operators to track recoveries for both the nightly CVS (CCV) and weekly LCS (SSCV) 1. The CVS worksheet is where the C of A of the standard connected to the blender

is entered and these values are linked to the Curve worksheet and Precision worksheet

2. The Blend ratio is automatically calculated based on the 3. Span and zero entered. 4. When the operator enters the concentrations found the recoveries are

automatically calculated

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Like the CVS worksheet there is also a worksheet for the LCS or SSCV.1. Operator must enter the C of A concentrations – There are fields to document

canister information and installation dates2. And the dilution used for the statically diluted canister when installed3. The recoveries are automatically calculated when concentrations from the

AutoGC are entered

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The precision worksheet is used for the weekly precision runs which are automatically run each week. 1. All fields reference other pages so only the concentrations measured are entered.

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With this configuration Data Acquisition is controlled by EZChrom. The Thermal Desorber is run in a single line sequence setup to a single port.

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The continuous monitoring is achieved using a sequence in EZChrom which queues up each sample. The sequence allows the data system to give each ambient sample 1. a unique filename2. A descriptive sample ID.3. And provide some descriptive information like the actual sample collection hourGenerating such a sequence with the necessary automation would be time consuming so we have provided a visual basic program called EZSequence for the generation of this file.

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EZSequence requires some input information to generate the necessary sequence1. Sequence type is Agilent2. QC type Static will put a check standard and Blank alternately on hour 00 and 01

or 02 and 03. Weekly CS and IQ will put the second source standard and RTS QC samples in hour 22 and 23 on Saturday night – precision runs are always on Sunday morning

3. Site ID is the 2 letter designation for the site used to name all the data files.4. You will designate the output directory for the generated csv file and the EZChrom

method name.5. External valving should be checked when using the Dilution system and MMSI

sample interface.6. When you hit apply EZ Sequence will generate the necessary csv file7. And a box will reveal the end date of the sequence –8. YOU MAY WANT TO MAKE A NOT OF THIS

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This is what the csv file looks like.

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To actually make the sequence 1. you will open the offline instrument and use File open to open the csv file from

the RU-other folder

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Select the csv file from the RU-other directory

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This will automatically import the csv into the EZChrom sequence editor.1. Once it is displayed you will use File save as to save the file as a *.seq file

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1. If using the mmove file archiving software you must be sure to save this file in the RU-meth folder so it is always archived with the daily data.

2. Use a consistent naming scheme such as shown here 3. with the year, month, day and site designator YYMMDD-RU

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The sequence is built with the necessary automation which adds events which control the relays necessary to trigger the QC samples. The Orsat Mvalve application controls valving for automated sample introduction1. The Action column is where the Mvalve executable programs are designated

which set the necessary valve configurations to achieve the desired quality control sample collection

2. Note that the valve configuration and event relays must be setup in the sample 2 hours prior to the actual chromatographic run, thus the filename and

3. sample ID is two rows offset from the entered executable4. This is done for nightly samples which are staggered every other day and for

weekly RTS and LCS samples5. As well as Sunday precision samples

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Once the EZChrom sequence has been built the Markes Instrument control is used to start the sampling. The Unity is also operated from a sequence but it is a one-line repeating sequence. From the Markes Instrument Control , left click on the sequence button to view the sequence dialog box.

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This is where the user will give the TD collection instructions1. The default view will load the Edit tab2. Choose sample in the sample type field3. Double left click here to choose your sampling method4. The channel is the air server channel that is collecting the sample5. The Sample Gas drop down will give you the choice of air, He, or N2. We are

sampling in air.

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Right click in the method field to select view method

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Double Check the method parameters and then click ok.

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1. On the edit tab use the green arrow icon to initiate the sequence.2. The Run sequence dialog will appear3. Place a check in the repeat and select continuous4. Then check Delay sequence Start and enter the start time. This time may be up to

5 minutes before the top of the hour to allow for leak testing and equilibration.

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The system will do its leak checks and equilibrate. It will then wait for the delay time before starting sampling. Since the sample collection is 40 min. You now have time to setup your GC sequence in EZChrom

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In the EZChrom online instrument – open the new sequence1. Determine the row for the data sample you just initiated in your new sequence. 2. Note the sequence row should be the row with the description field which

matches the sample collection hour you just started on the thermal desorber. 3. If you intend to run any unscheduled QC samples, you must enter the necessary

executables and identify the sample IDs and filenames two rows later. If you make changes make sure you save your sequence file.

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1. To initiate acquisition select the Control drop-down and choose Sequence Run.

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The Sequence run dialog box will open.1. Use the file icon to browse for your sequence file.2. Enter a result name – the date and row number will allow a new result to be

generated and allow additional result files as the sequence is started and stopped. This result name has to be unique each time your sequence is restarted.

3. Enter the range of rows you will run. Each sequences has 576 rows so each time you restart the same sequence you will change the first number to reflect the new starting row

4. Press start to start acquisition

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EZChrom will initialize the GC and then will show 1. “Waiting for Trigger” at the bottom of the window which indicates it will start on

the specified row when the Thermal Desorber sends the start signal to the GC.

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Agilent PAMS AutoGC Training

NAAMC 2018

Orsat has been configuring, operating and helping to develop quality controls to produce robust PAMS AutoGC systems since 1992. TCEQ currently collects data from 37 PAMS AutoGC systems in Texas which are operated year round. These systems post hourly data to the TCEQ website real-time with percent data recoveries of 90-95% and are operated based on the original PAMS technical assistance document which outlined using the average carbon response factor.

99NAAMC 2018


100NAAMC 2018


this training is designed to familiarize you with all the ... · agilent pams autogc training...

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