terrestrial/aquatic microbes at neon

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The National Ecological Observatory Network is a project sponsored by the National Science Foundation and managed under cooperative agreement by NEON Inc.

2012 National Ecological Observatory Network, Inc. ALL RIGHTS RESERVED.

Science Design for Terrestrial microbial Ecology at NEONJacob Parnell 2012 National Ecological Observatory Network, Inc. ALL RIGHTS RESERVED.

Terrestrial Observation System (TOS) Field Design Climate change Land-use dynamics Invasive species

Biogeochemical cycles Biodiversity and ecosystem function Hydrological forecasting Infectious diseases and environmentDrivers of biological and ecological changeEffects on organisms, populations, and communitiesResponsesDrivers

2012 National Ecological Observatory Network, Inc. ALL RIGHTS RESERVED.

NEON has divided the U.S, including Hawaii, AK, and Puerto Rico, into 20 domains that best encompass particular ecosystem characteristics. Within those domains, NEON has strategically located 60 sites using a sampling algorithm. Together, the 60 sites over time are expected to provided an accurate continental snapshot of continental-scale ecology.3Terrestrial Observation System (TOS) Field Design

2012 National Ecological Observatory Network, Inc. ALL RIGHTS RESERVED.

NEON has divided the U.S, including Hawaii, AK, and Puerto Rico, into 20 domains that best encompass particular ecosystem characteristics. Within those domains, NEON has strategically located 60 sites using a sampling algorithm. Together, the 60 sites over time are expected to provided an accurate continental snapshot of continental-scale ecology.4Terrestrial Observation System (TOS) Field Design1012Time [s]1001031061091012Length [m]10010-3103106109

Terrestrial & aquatic field samplingTerrestrial & aquatic sensorsAirborne remote sensing Satellite and external dataMetabolic processes, CO2, O2, H2O exchange Phenology, productivityPopulation dynamics, gas and nutrient fluxesSuccession, biodiversity Biogeochemical cyclesLand surface interactions500+ data products 2012 National Ecological Observatory Network, Inc. ALL RIGHTS RESERVED.

NEON has divided the U.S, including Hawaii, AK, and Puerto Rico, into 20 domains that best encompass particular ecosystem characteristics. Within those domains, NEON has strategically located 60 sites using a sampling algorithm. Together, the 60 sites over time are expected to provided an accurate continental snapshot of continental-scale ecology.5What to measure & Frequency of measurements

2012 National Ecological Observatory Network, Inc. ALL RIGHTS RESERVED.

NEON has divided the U.S, including Hawaii, AK, and Puerto Rico, into 20 domains that best encompass particular ecosystem characteristics. Within those domains, NEON has strategically located 60 sites using a sampling algorithm. Together, the 60 sites over time are expected to provided an accurate continental snapshot of continental-scale ecology.6What to measure & Frequency of measurements

For the first 2 years, samples will becollected every month in order to determine seasonal variation at eachsite. Three time points will be selectedthat maximize the seasonal variabilityfor the duration of the project. 2012 National Ecological Observatory Network, Inc. ALL RIGHTS RESERVED.

NEON has divided the U.S, including Hawaii, AK, and Puerto Rico, into 20 domains that best encompass particular ecosystem characteristics. Within those domains, NEON has strategically located 60 sites using a sampling algorithm. Together, the 60 sites over time are expected to provided an accurate continental snapshot of continental-scale ecology.7What to measure & Frequency of measurementsMaterial16SITSMeta-transcriptomeMeta-genomeqPCRCell countSoil540054005400180054000Water324324324108324324Sediment324324324108324324Benthic324324324108324324Total/yr63726372637221246372972Total1911601911601911606372019116029160 2012 National Ecological Observatory Network, Inc. ALL RIGHTS RESERVED.

NEON has divided the U.S, including Hawaii, AK, and Puerto Rico, into 20 domains that best encompass particular ecosystem characteristics. Within those domains, NEON has strategically located 60 sites using a sampling algorithm. Together, the 60 sites over time are expected to provided an accurate continental snapshot of continental-scale ecology.8Spatial design:

2012 National Ecological Observatory Network, Inc. ALL RIGHTS RESERVED.

NEON has divided the U.S, including Hawaii, AK, and Puerto Rico, into 20 domains that best encompass particular ecosystem characteristics. Within those domains, NEON has strategically located 60 sites using a sampling algorithm. Together, the 60 sites over time are expected to provided an accurate continental snapshot of continental-scale ecology.9Spatial design:

2012 National Ecological Observatory Network, Inc. ALL RIGHTS RESERVED.

NEON has divided the U.S, including Hawaii, AK, and Puerto Rico, into 20 domains that best encompass particular ecosystem characteristics. Within those domains, NEON has strategically located 60 sites using a sampling algorithm. Together, the 60 sites over time are expected to provided an accurate continental snapshot of continental-scale ecology.10

2012 National Ecological Observatory Network, Inc. ALL RIGHTS RESERVED.

Terrestrial Observation System (TOS) Field Design40m40m 2012 National Ecological Observatory Network, Inc. ALL RIGHTS RESERVED.

NEON has divided the U.S, including Hawaii, AK, and Puerto Rico, into 20 domains that best encompass particular ecosystem characteristics. Within those domains, NEON has strategically located 60 sites using a sampling algorithm. Together, the 60 sites over time are expected to provided an accurate continental snapshot of continental-scale ecology.12Aquatic Observation System (AOS) Field Design

2012 National Ecological Observatory Network, Inc. ALL RIGHTS RESERVED.

NEON has divided the U.S, including Hawaii, AK, and Puerto Rico, into 20 domains that best encompass particular ecosystem characteristics. Within those domains, NEON has strategically located 60 sites using a sampling algorithm. Together, the 60 sites over time are expected to provided an accurate continental snapshot of continental-scale ecology.13AcknowledgementsMicrobial Working Group:G. King, L. Kinkel, L. Zeglin, C. Blackwood, N. Fierer, J. GilbertM. Allen, J. Tiedje, E. Triplett, D. NemergutCurrent/Previous NEON Scientists:R. Gallery, K. Docherty, E. Hinckley, C. Meier, D. Barnett

2012 National Ecological Observatory Network, Inc. ALL RIGHTS RESERVED.

NEON has divided the U.S, including Hawaii, AK, and Puerto Rico, into 20 domains that best encompass particular ecosystem characteristics. Within those domains, NEON has strategically located 60 sites using a sampling algorithm. Together, the 60 sites over time are expected to provided an accurate continental snapshot of continental-scale ecology.14