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National Aeronautics and Space Administration www.nasa.gov Responseof Staphylococcusaureus toSimulatedMicrogravity https://ntrs.nasa.gov/search.jsp?R=20140010671 2018-06-28T07:28:56+00:00Z

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Page 1: Response of Staphylococcus aureus to Simulated Microgravity ·  Response of Staphylococcus aureus to Simulated Microgravity ... Duane Pierson, Ph.D. Chief NASA Microbiologist

National Aeronautics and Space Administration

www.nasa.gov

Response of Staphylococcus aureus to Simulated Microgravity 

https://ntrs.nasa.gov/search.jsp?R=20140010671 2018-06-28T07:28:56+00:00Z

Page 2: Response of Staphylococcus aureus to Simulated Microgravity ·  Response of Staphylococcus aureus to Simulated Microgravity ... Duane Pierson, Ph.D. Chief NASA Microbiologist
Page 3: Response of Staphylococcus aureus to Simulated Microgravity ·  Response of Staphylococcus aureus to Simulated Microgravity ... Duane Pierson, Ph.D. Chief NASA Microbiologist
Page 4: Response of Staphylococcus aureus to Simulated Microgravity ·  Response of Staphylococcus aureus to Simulated Microgravity ... Duane Pierson, Ph.D. Chief NASA Microbiologist

Debbie Aldape Kami Faust

Catherine Ballard Sarah Foster

Michelle Blackwell Jane McCourt

Doug Botkin, Ph.D. Satish Mehta, Ph.D.

Bekki Bruce  Cherie Oubre, Ph.D.

Victoria Castro Melanie Smith

Todd Elliott Airan Yoets

Goal: Mitigate microbial risk to crew health, safety, and performance during the human exploration of space

Duane Pierson, Ph.D.Chief NASA 

Microbiologist 

Mark Ott, Ph.D.

Sarah Castro, Ph.D.

Page 5: Response of Staphylococcus aureus to Simulated Microgravity ·  Response of Staphylococcus aureus to Simulated Microgravity ... Duane Pierson, Ph.D. Chief NASA Microbiologist
Page 6: Response of Staphylococcus aureus to Simulated Microgravity ·  Response of Staphylococcus aureus to Simulated Microgravity ... Duane Pierson, Ph.D. Chief NASA Microbiologist
Page 7: Response of Staphylococcus aureus to Simulated Microgravity ·  Response of Staphylococcus aureus to Simulated Microgravity ... Duane Pierson, Ph.D. Chief NASA Microbiologist
Page 8: Response of Staphylococcus aureus to Simulated Microgravity ·  Response of Staphylococcus aureus to Simulated Microgravity ... Duane Pierson, Ph.D. Chief NASA Microbiologist
Page 9: Response of Staphylococcus aureus to Simulated Microgravity ·  Response of Staphylococcus aureus to Simulated Microgravity ... Duane Pierson, Ph.D. Chief NASA Microbiologist
Page 10: Response of Staphylococcus aureus to Simulated Microgravity ·  Response of Staphylococcus aureus to Simulated Microgravity ... Duane Pierson, Ph.D. Chief NASA Microbiologist
Page 11: Response of Staphylococcus aureus to Simulated Microgravity ·  Response of Staphylococcus aureus to Simulated Microgravity ... Duane Pierson, Ph.D. Chief NASA Microbiologist

Absorbance at 460 nm

LSMMG Control Ratio

0.053 0.080 .663

0.116 0.307 .378

0.092 0.169 .544

0.099 0.160 .615

Average                   .550

Standard Dev        .125

LSMMG and Control values are statistically significant. p < .05

Page 12: Response of Staphylococcus aureus to Simulated Microgravity ·  Response of Staphylococcus aureus to Simulated Microgravity ... Duane Pierson, Ph.D. Chief NASA Microbiologist

* *

LSMMG induced mutation or just altered expression?

Page 13: Response of Staphylococcus aureus to Simulated Microgravity ·  Response of Staphylococcus aureus to Simulated Microgravity ... Duane Pierson, Ph.D. Chief NASA Microbiologist
Page 14: Response of Staphylococcus aureus to Simulated Microgravity ·  Response of Staphylococcus aureus to Simulated Microgravity ... Duane Pierson, Ph.D. Chief NASA Microbiologist

S. epidermidisB. subtilis

Page 15: Response of Staphylococcus aureus to Simulated Microgravity ·  Response of Staphylococcus aureus to Simulated Microgravity ... Duane Pierson, Ph.D. Chief NASA Microbiologist
Page 16: Response of Staphylococcus aureus to Simulated Microgravity ·  Response of Staphylococcus aureus to Simulated Microgravity ... Duane Pierson, Ph.D. Chief NASA Microbiologist
Page 17: Response of Staphylococcus aureus to Simulated Microgravity ·  Response of Staphylococcus aureus to Simulated Microgravity ... Duane Pierson, Ph.D. Chief NASA Microbiologist