oto doroghazi - effect of contamination of maize seeds with lead on seed biological value (winner...
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UTICAJ KONTAMINACIJE SEMENA KUKURUZA OLOVOM NA NJEGOVU
BIOLOŠKU VREDNOSTEffect of contamination of maize seeds with lead on seed
biological value
Autor: Otto DOROGHAZI, IV godina studijaMentor: Prof. dr Ivana Maksimović
Institucija: Univerzitet u Novom Sadu, Poljoprivredni fakultet,
smer za Ratarstvo i Povrtarstvo, Novi Sad
Pb u prirodiPb in enviroment
Otpaci urbanih sredinaUbran wastes
Izduvni gasovi saobraćajnih sredstvaExhaust of automobiles
Aditivi u bojiAdditives in paints
Veštačka đubriva i pesticidi
Fertilizers, pesticides
Industrijska postrojenja
IndustryFabrike akumulatora i
njihovi otpaci Effluents from battery
industry
RudeOre
KONTAMINACIJA SEMENA Pb (Seed contamination with lead)
KONTAMINACIJA SEMENA Pb (Seed contamination with lead)
KONTAMINACIJA SEMENA Pb (Seed contamination with lead)
KONTAMINACIJA SEMENA Pb (Seed contamination with lead)
KONTAMINACIJA SEMENA Pb (Seed contamination with lead)
KONTAMINACIJA SEMENA Pb (Seed contamination with lead)
[molPb/dm3]
[cm
]
Početna hipotezaInitial hypothesis
Lead concentration
Plan
t hi
ght
Pb
Pb u prirodiPb in enviroment
Otpaci urbanih sredinaUbran wastes
Izduvni gasovi saobraćajnih sredstvaExhaust of automobiles
Aditivi u bojiAdditives in paints
Veštačka đubriva i pesticidi
Fertilizers, pesticides
Industrijska postrojenja
IndustryFabrike akumulatora i
njihovi otpaci Effluents from battery
industry
RudeOre
Struktura ćelijskih membrana
(Membrane structure)
Elongacija ćelija
(Cell elongation)
Vodni režim(Water regime)
Enzimska Aktivnost
(Enzyme activity)
Mitoza(Mitosis)
Pb2+Mineralna ishrana
(Mineral nutrition)
Rastenje ponika
(Growth of seedlings)
Fotosinteza(Photosynthesis) -
--
-
-
--
-/+
Pb2+
[molPb/dm3]
[cm
]Početna hipoteza
Initial hypothesis
Pb2+ Pb2+ Pb2+Pb2+
Lead concentration
Plan
t hi
ght
Materijal i metod radaMaterial and Methods
1) KONTAMINACIJA SEMENA Pb (Seed contamination with lead)
2) BIOLOŠKA VREDNOST SEMENA (Seed biological value of seedlings)
3) ODREĐIVANJE PORASTA I SUVE MASE PONIKA (Determination of maize seedlings growth and dry mass)
4) HEMIJSKA ANALIZA (Chemical analysis)5) STATISTIKA (Statistics)
1. KONTAMINACIJA SEMENA OLOVOMContamination of seeds with lead
NS HIBRID 7016 BUBRILO U RAZLIČITIM PbCl2 RASTVORIMA (Swelled
in different PbCl2 solutions):
10-410-5 10-210-3 KONTROLA
0, 10-5, 10-4, 10-3, 10-2 mol Pb/dm3
2. ODREĐIVANJE BIOLOŠKE VREDNOSTI SEMENA
Determination of seed biological value
• NAKLIJAVANJE SEMENA (Germination)
• ENERGIJA KLIJANJA (Vigor)
• KLIJAVOST (Seed germination)
• BROJ TIPIČNIH PONIKA (Percent of typic young seedlings)
• BROJ ATIPIČNIH PONIKA (Percent of untypic seedlings)
• NEPROKLIJALO SEME (Ungerminated seeds)
3. ODREĐIVANJE PORASTA I SUVE MASE PONIKA Determination of maize seedlings growth and dry mass
DUŽINA IZDANKA (Shoot length)
DUŽINA PRIMARNOG KORENA (Primary root length)
DUŽINA KORENA MEZOKITILA (Mesocotyl root length)
SUVA MASA IZDANKA I KORENA (shoot and root dry matter mass)
List (Leaf)
Koleoptila(Coleoptile)
Primarni koren(Primary root)
Koren mezokotila (Mesocotyl root)
Izdanak (Shoot)
Koren (Root)
4. HEMIJSKA ANALIZAChemical analysis
ccHNO3 + ccH2O2
KJELDHAL METOD → N ICP → P, K, S, Mg, Ca, Na, Fe,
Zn, Cu, Mn, Mo, Pb
• LSD 95%• LSD 99%
5. STATISTIKAStatistics
Dinamika usvajanja vode od strane semena kukuruza Dinamics of water uptake by maize seed
Merenja (časovi) Hours
Sadr
žaj v
ode
(%)
Wat
er c
onte
nt
REZULTATI - Results
0
5
10
15
20
25
30
35
0 2 4 6 8 10 12 14
Sadr
žaj o
lova
(m
g/kg
) Le
ad
cont
ent
10-5 10-4 10-3 10-2
Koncentracija olova (mol/dm3) Lead concentration
Koren (Root)
Izdanak (Shoot)
Sadržaj Pb u pojedinim biljnim delovima pri različitim kontaminacijama semena sa Pb (mg/kg SM)
Pb contents in different plant partition after seed contamination with different Pb tretmants (mg/kg DM)
0
20
40
60
80
100
120
140
160
180
200
BIOLOŠKA VREDNOST SEMENA
The biological value of seed
Parametri Parameters
Koncentracija Pb (mol/dm3)Concentration of Pb (mol/dm3)
Ø 10–5 10–4 10–3 10–2
Tipičan ponik (%)(Typic young seedlings) 96,75 95,50 95,00 94,75 93,75
Atipičan ponik (%)(Untypic young seedlings) 2,25 2,75 4,50 4,00 4,75
Neproklijalo seme (%)(Ungerminated seed) 1,00 1,75 0,50 1,25 1,25
Energija klijanja (%)(Vigor) 97,00 95,00 95,00 95,00 94,00
Klijavost (%)Germination 97,00 95,00 95,00 95,00 94,00
Uticaj Pb na rastenje ponikaEffect of different Pb
concentrations on maize initial growth
Parametri Parameters
Koncentracija Pb (mol/dm3)Concentration of Pb (mol/dm3)
Ø 10–5 10–4 10–3 10–2
Dužina izdanka (cm)(Length of shoot) 10,13 10,26 9,90 10,49* 9,85
Dužina primarnog korena (cm)(Length of primary root) 12,11 11,80 11,70 11,91 11,20**
Dužina korena mezokotila (cm)(Length of mesocotyl root) 9,20 9,03 8,89 9,37 8,61**
Uticaj Pb na suvu masu izdanaka i korena ponikaEffect of different Pb concentrations on
seedlings shoot and root dry mass
Parametri Parameters
Koncentracija olova (mol/dm3)Concentration of lead (mol/dm3)
Ø 10–5 10–4 10–3 10–2
Suva masa izdanka (mg)(Dry matter mass of shoot) 28,30 27,00 28,10 27,8 28,70
Suva masa korena (mg)(Dry matter mass of root) 36,50 33,10 39,50 36,5 29,90*
N KP
Mg
S
Na Ca
Zn
Fe
Mn
Cu
Mo (%)
Translokacija pojedinih elemenata iz semena u izdanak i u koren
Translocation of elements from seed to the shoot and root
Izda
nak
(Sho
ot)
Kore
n (R
oot)
izdanak
izdanak
izdanak
izdanak
izdanak
izdanak izdanak
koren
koren
korenkoren
koren
koren
koren
Kontrola
N P K S Mg Ca Na
Statistički zančajna razlika (99%)
Traslokacija makroelemenata Translocation of macroelements
shoot
shoot shoot
shoot
shoot
shoot shootroot
root
root
root root root
root
(%)
(%)
ZAKLJUČAK (Conclusions)
• Akumulacija Pb je bilo veća u korenu (The acumulation of lead was higher in root then shoots)
• Smanjila se klijavost, energija klijanja i broj tipičnih ponika (Decrease the germination, vigor, and the number of typic seedlings)
• Porastao je broj atipčnih ponika (Increase the number of untypic seedlings)
• Suva masa izdanaka i dužina izdanka nisu značajnije promenili (Shoot length and dry mass did not change singnificantly)
• Dužina primarnog korena, korena mezokotila i suva masa korena se značajno menja u poređenju sa kontrolom (Length of primary root, mesocotyl root and dry matter of root changed singnificantly)
• Remobilizacija pojedijnih elemenata je bila različita (Remobilization of elements was different)
• Tolerancija (Tolerance)• Plan budućih istraživanja (The plan for the future)
Hvala van na pažnji!Thanks for your attention!
Zahvalonost dugujem ljudima koji su mi pomogli u radu:
Mentori: Prof. dr Ivana Maksimović
Akademik Rudolf Kastori
Laboranti: Katica Gavrančić
Nada Rihter