Multilevel analysis towards drought tolerance in Legumes

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Multilevel analysis towards drought tolerance in Legumes . [ P23441-B20 ]. Start: 2011 Stop : 2014 . PhD Students : Christiana Staudinger: Plant Physiology , Proteomics and Metabolomics Vlora Mehmeti: Plant Physiology and Metabolomics David Lyon: - PowerPoint PPT Presentation



Multilevel analysis towards drought tolerance in Legumes


Start: 2011 Stop: 2014 One of the most crucial functions of plant cells is their ability to respond to alterations in their environment. Understanding the connections between initial responses and the downstream events that constitute successful adjustment to its fluctuating environment is one of the challenges of plant biology research. Investigations on transcript level are the most common studies so far. Besides transcript analyses, new technologies based on mass spectrometry allow for the comprehensive study on metabolite and protein level. Past studies have focussed on different stresses such as temperature, drought or salt using various plants and technologies. All these data have improved the understanding on the complexity of the plant response depending on the intensity and duration of homeostatic perturbation. However, due to the diversity of the research data and experimental conditions a comparison and integration is difficult or even impossible. Thus to gain better insights and to be able to visualise the complexity of the plant respond, integrative analyses combining different technologies and standardised cultivation conditions are becoming necessary. Legumes are major sources of vegetable protein and indispensable for sustainable agriculture due to their ability to fix atmospheric nitrogen via their symbiosis with soil rhizobia. These bacteria colonize legume roots in specialized organs called nodules. However, a full understanding of the interactive regulatory mechanisms between plant and bacteroids towards increased stress tolerance is not accomplished so far. To contribute to a deeper understanding of responses of legumes to major constraints, the proposed project aims at employing comparative systems biology. Supported by bioinformatic modelling strategies, novel metabolic and proteomic key mechanisms that may serve as regulatory targets for improving stress-tolerance in legumes will be identify. Link to Conferences and Posters Link to PublicationsPhD Students:

Christiana Staudinger:

Plant Physiology, Proteomics and Metabolomics

Vlora Mehmeti:

Plant Physiology and Metabolomics

David Lyon:

Proteomics, Metabolomics and BioinformaticsGuest Scientists

Erena Gil (Universidad Publica de Navarra): Proteomics; Medicago and Soybean nodules; drought stress.

Dr. M.Angeles Castillejo, Universidad de Cordoba (funded by the Spanish Ministry of Science and Innovation): Plant Physiology and Proteomics; 15N-metabolic labelling

Veronica Castaeda (Universidad Publica de Navarra): Proteomics; Medicago root plasma membranes; drought stressPI: Dr. Stefanie Wienkoop

Bioinformatic supervision: Dr. Volker Egelhofer

Multilevel analysis towards drought tolerance in Legumes


Publications: Peer Rev. Journals:

- D. Lyon, M.A. Castillejo, C. Staudinger, W. Weckwerth, S.Wienkoop and V. Egelhofer (2014). Automated protein turnover calculations from 15N partial metabolic labeling LC/MS shotgun proteomics data. Plos One, 9(4): e94692. doi: 10.1371/journal.pone.0094692.

- C. Staudinger, V. Mehmeti, R. Turetschek, D. Lyon, V. Egelhofer and S. Wienkoop (2012). Possible role of nutritional priming for early salt and drought stress responses in Medicago truncatula. Frontiers Plant Proteomics. doi: 10.3389/fpls.2012.00285

- A. Mari, D. Lyon, L. Fragner, P. Montoro, S. Piacente, S. Wienkoop, V. Egelhofer, W. Weckwerth (2012). Phytochemical composition of Potentilla anserina L. analyzed by an integrative GC/MS and LC/MS metabolomics platform. Metabolomics Doi:10.1007/s11306-012-0473-x

Book chapters:

- M.A. Castillejo, C. Staudinger, V. Egelhofer and S. Wienkoop (2014). Medicago truncatula proteomics for systems biology: novel rapid shotgun LC-MS approach for relative quantification based on Full-Scan Selective Peptide Extraction (Selpex). Chapter 22 In: Plant Proteomics Methods and Protocols. Ed. J.V. Jorrin Novo, S. Komatsu, W. Weckwerth, S. Wienkoop, : Springer New York. Download: Publication: Download: publications still in progress!Multilevel analysis towards drought tolerance in Legumes


Conferences and Posters: 2013

17. 21.3. Proteomics Formum 2013, Berlin, Germany a) D. Lyon, M.A. Castillejo, C. Staudinger, V. Mehmeti, V. Egelhofer and S. WienkoopProtein turnover calculations from partial metabolic labeling in Planta experiments.

b) Staudinger Christiana, Castillejo Maria-Angeles, Mehmeti Vlora, Lyon David, Egelhofer Volker, Wienkoop StefanieCombined Label-free Quantification Techniques to Detect Water Deficit Induced Changes in the Medicago truncatula Proteome8. 13.6. 61st ASMS Conference, Minneapolis, MN, USAD. Lyon, S. Wienkoop, V. EgelhoferAn automated program for protein turnover calculations from LC/MS shotgun proteomics data resulting from partial metabolic labelling experiments.4. - 10.08. 7th European Summer School advanced proteomics, Kloster Neustift, Brixen, South Tirol, ItalyStaudinger C., Castellejo-Sanchez MA., Lyon D., Egelhofer V. and Wienkoop S. Suffer and recover: How Medicago truncatula acclimates to water deficit

2. - 6.09. Interdrought-IV, Crown Perth, Western Australia Staudinger C., Turetschek R., Mehmeti V., Castillejo M., Lyon D. and Wienkoop S. Legume nitrogen nutrition-related strategies to cope with drought2012

18.-21.2. ORAL PRESENTATION - VIPCA, Internat. Conf. Plant Growth, Nutrition & Environment Interactions, Vienna, Austria V. Mehmeti, S. WienkoopMolecular Changes in the metabolome of Medicago truncatula Induced by Short Term Salt and Drought Stress.22.-25.2. VIPCA, Intern. Conf. Plant Abiotoc Stress Tolerance IIC, Vienna, Austria.C. Staudinger, V. Mehmeti, E. Gil, E.M.Gonzalez, S. Wienkoop Rhizobial Symbiosis-related Effects on Drought Tolerance in Medicago truncatula.

2.-5.09. 10th European Nitrogen Fixation Conference, Munich, Germanya) Staudinger Christiana, Mehmeti Vlora, Gil Erena, Gonzales Esther and Wienkoop Stefanie Proteomic characterisation of microsymbtiont-dependent changes in Medicago truncatula roots and shoots.

b) V. Mehmeti, C. Staudinger and S. WienkoopDIFFERENCIAL NITROGEN SOURCE ASSOCIATED METABOLITE CHANGES OF MEDICAGO TRUNCATULA INDUCED BY SHORT TERM SALT AND MILD DROUGHT STRESSLink Poster 1Link Poster 3Link Poster 4Link Poster 5Link Poster 2Link Poster 6Link Poster 7Link Poster 8