make msa1 fragment msa1 precip msa1 resuspend msa1 hyb ...€¦ · bc2 #5 bc2 #1 a1 b1 c1 d1 e3 f3...

Title: Infinium HD Assay Super Lab Tracking Form Part Number: 11327261

Revision: C Effective Date: 06/15/10

1 of 7

Template No: 11253484 Rev D Proprietary & Confidential—For Internal Use Only

1 Make and Read Quant (Optional/LIMS) Date/Time: ____________________________________________ WG#-DNA Plate: QNT Plate: Operator: ___________________ Robot: ___________________ (1) ___________________ _____________________ (2) ___________________ _____________________ (3) ___________________ _____________________

Standard DNA Plate: _____________________

Standard QNT Plate: _____________________

2 Make MSA1 Date/Time: ____________________________________________ WG#-DNA Plate: ____________________________ Operator: ___________________ Robot: ___________________ MSA1 Plate: ________________________________ Plate Positions on Robot Bed: ___________________________ MA1 Reagent: ______________________________ Batch #: _______________________________________________ MA2 Reagent:_______________________________ Number of Samples: 48 / 96 MSM Reagent: ______________________________ Hyb oven (37°C, 20-24 h): Start __________ Stop __________ Record WG#-DNA sample IDs in table on page 3.

3 Fragment MSA1 Date/Time: ____________________________________________ FMS Reagent: _______________________________ Operator: ___________________ Robot: ___________________ Plate Positions on Robot Bed: ___________________________ Vortex at 1600 rpm Heat block (37°C, 1 h): Start ___________ Stop ____________

4 Precip MSA1 Date/Time: ____________________________________________ 2-propanol Lot #: _____________________________ Operator: ___________________ Robot: ___________________ 2-propanol Date Opened: _____________________ Plate Positions on Robot Bed: ___________________________ PM1 Reagent: ________________________________ Vortex at 1600 rpm Heat block (37°C, 5m): __________________________________

Incubate (4°C, 30m): Start: ____________ Stop: ____________

Air dray (22°C, 1 h): Start: ____________ Stop: _____________

5 Resuspend MSA1 Date/Time: ____________________________________________ RA1 Reagent: _______________________________ Operator: ___________________ Robot: ___________________ Plate Positions on Robot Bed: ___________________________ Hyb oven (48°C, 1 h): Start ___________ Stop _____________

6 Hyb Multi BC2 Date/Time: ____________________________________________ PB2 Reagent: ________________________________ Operator: ___________________ Robot: ___________________ ________________________________

Project: _______________ Batch: _______________ M

SA1 Plate: _______________ Im

age Date: _______________



2 of 7


Vortex at 1800 rpm ________________________________ Heat block (95°C, 20m): Start: ___________ Stop: __________

Refer to pages 4–6 for BeadChip loading instructions. Enter the BeadChip barcodes in the spaces provided.

Centrifuge MSA1 plate to 280 xg Hyb oven (48°C, 16-24 h): Start _________ Stop ___________

7 Wash BeadChip Date/Time: ____________________________________________ PB1 Reagent: ________________________________ Operator: ___________________ Robot: ___________________ ________________________________ ________________________________

8 XStain HD BeadChip Date/Time: ____________________________________________ RA1 Reagent: ________________________________ Operator: ___________________ Robot: ___________________ XC1 Reagent: (1-8) ___________________________

Record the chamber rack position for each BeadChip on page 7. (9-16) __________________________ (17-24) _________________________ XC2 Reagent: (1-8) ___________________________ (9-16) __________________________ (17-24) _________________________ TEM Reagent: (1-8) ___________________________ (9-16) __________________________ (17-24) _________________________ XC3 Reagent: ________________________________ STM Reagent: (1-8) ___________________________ (9-16) __________________________ (17-24) _________________________ STM Temperature: ____________________________ ATM Reagent: (1-8) ___________________________ (9-16) __________________________ (17-24) _________________________ PB1 Reagent: ________________________________ XC4 Reagent: ________________________________

9 Image BeadChip Record the Scanner ID and the image date for each BeadChip on pages 4–6.



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Record DNA Sample IDs in the MSA1 Plate Columns 1–4 of Microtiter Plate

1 2 3 4

A ______________________________ ______________________________ ______________________________ ______________________________

B ______________________________ ______________________________ ______________________________ ______________________________

C ______________________________ ______________________________ ______________________________ ______________________________

D ______________________________ ______________________________ ______________________________ ______________________________

E ______________________________ ______________________________ ______________________________ ______________________________

F ______________________________ ______________________________ ______________________________ ______________________________

G ______________________________ ______________________________ ______________________________ ______________________________

H ______________________________ ______________________________ ______________________________ ______________________________

Columns 5–8 of Microtiter Plate

5 6 7 8

A ______________________________ ______________________________ ______________________________ ______________________________

B ______________________________ ______________________________ ______________________________ ______________________________

C ______________________________ ______________________________ ______________________________ ______________________________

D ______________________________ ______________________________ ______________________________ ______________________________

E ______________________________ ______________________________ ______________________________ ______________________________

F ______________________________ ______________________________ ______________________________ ______________________________

G ______________________________ ______________________________ ______________________________ ______________________________

H ______________________________ ______________________________ ______________________________ ______________________________

Columns 9–12 of Microtiter Plate

9 10 11 12

A ______________________________ ______________________________ ______________________________ ______________________________

B ______________________________ ______________________________ ______________________________ ______________________________

C ______________________________ ______________________________ ______________________________ ______________________________

D ______________________________ ______________________________ ______________________________ ______________________________

E ______________________________ ______________________________ ______________________________ ______________________________

F ______________________________ ______________________________ ______________________________ ______________________________

G ______________________________ ______________________________ ______________________________ ______________________________

H ______________________________ ______________________________ ______________________________ ______________________________



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Track BeadChips 1-24 for the 4x1 HD BeadChip

MSA1 Plate

WG

1123

4567

89-M

SA

1

1 2 3 4 5 6 7 8 9 10 11 12

A

B

C

D

E

F

G

H

OddNumberedBeadChips

EvenNumberedBeadChips

R03C01

R01C01

1123456789

R02C01

R04C01

Sample Section Naming Diagram

Barcode:

Scanner ID:

Image Date:

A3

B3

C3

D3

BC2 #5

BC2 #1

A1

B1

C1

D1

E3

F3

G3

H3

BC2 #2

BC2 #6

E1

F1

G1

H1

A4

B4

C4

D4

BC2 #7

BC2 #3

A2

B2

C2

D2

E4

F4

G4

H4

BC2 #4

BC2 #8

E2

F2

G2

H2

A5

B5

C5

D5

BC2 #9

E5

F5

G5

H5

BC2 #10

A6

B6

C6

D6

BC2 #11

E6

F6

G6

H6

BC2 #12

Robot Setup #1

BC2 #13

A7

B7

C7

D7

BC #14

E7

F7

G7

H7

BC2 #15

A8

B8

C8

D8

BC2 #16

E8

F8

G8

H8

A9

B9

C9

D9

BC2 #17

BC2 #21

E9

F9

G9

H9

BC2 #18

BC2 #22

BC2 #19

BC2 #23

BC2 #20

BC2 #24

A11

D11B11

C11 E11 G11

F11 H11

E12

F12

G12

H12

E10

F10

G10

H10

A10

B10

C10

D10

A12

B12

C12

D12

Robot Setup #2

1123456789 1123456789 1123456789

1123456789

1123456789

1123456789

11234567891123456789

1123456789

1123456789

1123456789

1123456789

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

1123456789

1123456789

1123456789

1123456789

1123456789

1123456789

1123456789

1123456789

1123456789

1123456789

1123456789

1123456789

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:



5 of 7


Track BeadChips 1-24 for the 4x3 HD BeadChip

MSA1 Plate

WG

1123

4567

89-M

SA

1

1 2 3 4 5 6 7 8 9 10 11 12

A

B

C

D

E

F

G

H

OddNumberedBeadChips

EvenNumberedBeadChips

R 0 1 C 0 2R 0 1 C 0 1

1123456789

R 0 2 C 0 2R 0 2 C 0 1


Barcode:

BeadArrayReader ID:

Image Date:

A3

B3

C3

D3

Barcode:

BeadArrayReader ID:

Image Date:

BC2 #5

1123456789 610Qv1HD

1123456789 610Qv1HD

BC2 #1

A1

B1

C1

D1

E3

F3

G3

H3

Barcode:

BeadArrayReader ID:

Image Date:

Barcode:

BeadArrayReader ID:

Image Date:

BC2 #2

BC2 #6

1123456789 610Qv1HD

1123456789 610Qv1HD

E1

F1

G1

H1

Barcode:

BeadArrayReader ID:

Image Date:

A4

B4

C4

D4

Barcode:

BeadArrayReader ID:

Image Date:

BC2 #7

1123456789 610Qv1HD

1123456789 610Qv1HD

BC2 #3

A2

B2

C2

D2

E4

F4

G4

H4

Barcode:

BeadArrayReader ID:

Image Date:

Barcode:

BeadArrayReader ID:

Image Date:

BC2 #4

BC2 #8

1123456789 610Qv1HD

1123456789 610Qv1HD

E2

F2

G2

H2

A5

B5

C5

D5

Barcode:

BeadArrayReader ID:

Image Date:1123456789 610Qv1HD

BC2 #9

E5

F5

G5

H5

Barcode:

BeadArrayReader ID:


BC2 #10

A6

B6

C6

D6

Barcode:

BeadArrayReader ID:


BC2 #11

E6

F6

G6

H6

Barcode:

BeadArrayReader ID:


BC2 #12

Robot Setup #1

Barcode:

BeadArrayReader ID:


BC2 #13

A7

B7

C7

D7

Barcode:

BeadArrayReader ID:

Image Date:

BC2 #14

1123456789 610Qv1HD

E7

F7

G7

H7

Barcode:

BeadArrayReader ID:


BC2 #15

A8

B8

C8

D8

Barcode:

BeadArrayReader ID:

Image Date:

BC2 #16

1123456789 610Qv1HD

E8

F8

G8

H8

A9

B9

C9

D9

Barcode:

BeadArrayReader ID:

Image Date:

Barcode:

BeadArrayReader ID:

Image Date:

BC2 #17

1123456789 610Qv1HD

BC2 #21

E9

F9

G9

H9

Barcode:

BeadArrayReader ID:

Image Date:

Barcode:

BeadArrayReader ID:

Image Date:

BC2 #18

1123456789 610Qv1HD

1123456789 610Qv1HD

BC2 #22

Barcode:

BeadArrayReader ID:

Image Date:

Barcode:

BeadArrayReader ID:

Image Date:

BC2 #19

1123456789 610Qv1HD

1123456789 610Qv1HD

BC2 #23

Barcode:

BeadArrayReader ID:

Image Date:

Barcode:

BeadArrayReader ID:

Image Date:

BC2 #20

1123456789 610Qv1HD

1123456789 610Qv1HD

BC2 #24

A11

D11B11

C11 E11 G11

F11 H11

1123456789 610Qv1HD

E12

F12

G12

H12

E10

F10

G10

H10

A10

B10

C10

D10

A12

B12

C12

D12

Robot Setup #2



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Track BeadChips 1-12 for the 8x1 HD BeadChip MSA1 Plate

WG

1123

4567

89-M

SA

1

1 2 3 4 5 6 7 8 9 10 11 12

A

B

C

D

E

F

G

H

R 0 1 C 0 1

R 0 2 C 0 1

R 0 3 C 0 1

R 0 4 C 0 1

R 0 5 C 0 1

R 0 6 C 0 1

R 0 7 C 0 1

R 0 8 C 0 1


Barcode:

Scanner ID:

Image Date:

BC2 #5

BC2 #1 BC2 #2

BC2 #6 BC2 #7

BC2 #3 BC2 #4

BC2 #8

BC2 #9 BC2 #10 BC2 #11 BC2 #12

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

Barcode:

Scanner ID:

Image Date:

1123456789

A1B1

E1F1

C1D1

G1H1

1123456789

A3B3

E3F3

C3D3

G3H3

1123456789

A2B2

E2F2

C2D2

G2H2

1123456789

A4B4

E4F4

C4D4

G4H4

1123456789

A5B5

E5F5

C5D5

G5H5

1123456789

A6B6

E6F6

C6D6

G6H6

1123456789

A7B7

E7F7

C7D7

G7H7

1123456789

A8B8

E8F8

C8D8

G8H8

1123456789

A9B9

E9F9

C9D9

G9H9

A11

1123456789

B11

C11D11

G11H11

E11F11

A12

1123456789

B12

C12D12

G12H12

E12F12

A10

1123456789

B10

C10D10

G10H10

E10F10



7 of 7


Chamber Rack Position Chart Use this chart to enter BeadChip IDs in the appropriate chamber rack position during the XStain HD BeadChip step.

Row 1 Row 2 Row 3

1

5

2

6

3

7

4

8

9

13

10

14

11

15

12

16

17

21

18

22

19

23

20

24

Release History Revision CO # Originator Description of Change

C 12952 E. Clark Incorporated new Illumina Template for this info.

make msa1 fragment msa1 precip msa1 resuspend msa1 hyb ...€¦ · bc2 #5 bc2 #1 a1 b1 c1 d1 e3 f3...

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