PHYTOCHEMICAL ANALYSIS

Nature of analysis Method

EXTRACTION OF PHYTOCHEMICALS Extraction Water Solvents Soxhlet extraction Water Solvents

Phytochemical analysis QUALITATIVE ANALYSIS

Phytochemical screening 10to 12 Tests Paper chromatography 2 to 4 solvents

TLC 4 solvents

Column chromatography Silica

Sephadex G OR DEAE cellulose

ANTIOXIDANT ACTIVITY UV Visible DPPH UV Visible

Hydrogen peroxide UV Visible FRAP UV Visible

Flavanoids/ Tannin UV Visible Total Phenol / Alkaloids UV Visible

Metal chelating UV Visible In vitro alpha amylase inhibition

assay UV Visible

In vitro α-glucosidase inhibition assay

UV Visible

Total falvonids

ANTIMICROBIAL ACTIVITY Per plate Anti fugal activity Per plate

Antimicrobial activity by TLC TLC plate HPLC for plant sample HPLC

HPTLC Compound identification GC MS With Library and

compound identification Anti cancerous activity

Using cell lines (one cell line)

Tryphan blue assay MTT Assay

DNA Damage activity Per sample Two cell line On request

Nature of analysis Method Nanoparticle synthesis

Types Gold Silver Zinc Copper Others

Characterization UV- VIS UV – VIS FTIR FTIR SEM SEM SEM AND EDX SEM and EDX

XRD XRD

Molecular Biology/ Recombinant / DNA finger Printing techniques

Nature of analysis Method DNA Gel electrophoresis ( Bacteria, Fungi or plant) With Gel doc

AGE

RNA AGE RFLP AGE RAPD ( Bacteria) - RAPD (Plant) ISSR AGE RAPD (Animal) ISSR AGE

Transformation - PCR alone Sequencing up to ~700 bases 16SRNAor 18 or 23sRNA

-

Gene cloning and selection of clones TA Primers (per nucleotide)

-

PROTEIN

Nature of analysis Method Salt ppt/ Dialysis/ and column chromatography

Down stream

Column chromatography Sephadex G OR DEAE cellulose

SDS- PAGE – with marker SDS Bio reductase assay SDS TLC Silica Gel HPLC for compound analysis HPLC GC alone GC

Anti cancerous/ Cyototoxicity assay

Nature of analysis Method

Using cell lines (one cell line) Tryphan blue assay

MTT Assay DNA Damage activity Per sample

Water/ Food analysis

Nature of analysis Method Food adulteration ( 5 to 6) UV- Visible /

Titration Microbial load (TVC) - Coli form test MPN and all the coliforms tests - Detection of Salmonella/ Shigella - Detection of Histamines HPLC Pathogens identification - Ash - Crude fiber Carbohydrate Fat Protein Fatty acids by TLC Fatty acids by GC-MS Aminoacids TLC Vitamin A Vitamin C/Ascorbic acid Folic acid TLC analysis of Vitamins

Mineral Analysis Iron UV Calcium UV Magnesium UV Potassium AAS Zinc AAS HPLC - GC MS - Please contact : 9629388728

cbnrcindia@gmail.com