agilent 6495 triple quadrupole lc/ms system 6495 triple quadrupole lc/ms system experience a new...

Agilent 6495 Triple Quadrupole LC/MS System

Experience A New Level of

Confidence

Agilent Technologies

7/31/2014

Agilent 6495 QQQ LC/MS System

1

Overview of Topics

Markets and Applications

Technology, performance, and differentiation

• Technology innovations

• New sensitivity performance standard: Instrument Detection Limit (IDL)

• Quantitation performance

• New software capabilities

Key Applications

• Food safety – Pesticides in food matrices

• Food safety – Estrogens in milk

• Environmental – Water analysis

• Peptide quantitation

Summary: Product Values

7/31/2014


2

6495 Markets and Applications

7/31/2014


3

• Biomarkers

• Peptide Quan

• High sensitivity

• Complex matrices

Protein Quant

• Vitamin D

• Endogenous steroids

Clinical Research

• Pesticides

• Mycotoxins

• Antibiotics

• Sample dilution

Food Safety

• Water

• Hormones

• Contaminants

• Direct injections

Environmental

• Research

• Chemical

• High sensitivity

Academia

• Drug discovery

• ADME/PK

• Bioanalysis

• Microdosing

Pharma

• Kits & Methods

• tMRM

Veterinary Drugs

• Drugs of abuse

• Designer drugs

• Oral fluids

Forensic Toxicology

Proven iFunnel Technology

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4

Agilent Jet Stream Hexabore Capillary Dual Ion Funnel

• Thermal gradient focusing

• Efficient desolvation

• Creates an ion rich zone

• Six capillary inlets

• Samples x10 times more

ion rich gas

• Removes the gas but

captures the ions

• Removes neutral noise

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5

6495 QQQ Technologies Continued Development

1 2

3

• New Detector with High Energy Conversion Dynode

• Improved NEG ion detection with low noise 3

• New Tapered Hexapole Collision Cell

• Effective ion collection and transmission 2

• New Enhanced Q1 Ion Optics

• Improved ion transmission 1

Proven iFunnel Technology • Agilent Jet Stream

• Hexabore Capillary

• Dual Ion Funnel

• Increased ion generation

• Enhanced ion sampling

Quantitative Applications

• Enhanced peak area response

• Improved peak area %RSD

• More sensitive and precise

• Lower Limits of Detection

(IDL)and Quantitation (LLOQ)

• More reliable and robust

New Enhanced Q1 Ion Optics Improved transmission of ions and system robustness

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6

1. Reduced Octopole length 1

1. New prefilter 2

1

2

• New optimized MS 1 prefilter geometry for improved precursor ion transmission

– Improved peak area response and peak area %RSD, more sensitive and precise

• New optical lens elements for reduced the probability of contamination and easier autotune

– More reliable and robust performance

New Tapered Hexapole Collision Cell Effective collection and transmission of ions

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• Curved and Tapered Hexapole Assembly

for efficient collection and transmission of

product ions

• Consistent collision cell pressure for

higher quality MS/MS spectra

• Designed for consistent collision energies

across all QQQ platforms

• Capable of higher RF voltage on rods for

higher mass transmission

New Detector with High Energy Conversion Dynode More efficient detection of ions with low noise characteristics

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• Improved ion detection efficiency with High Energy Dynode (HED) voltage up to 20 kV

- Improved peak area response and peak area %RSD in positive & negative ion mode

- Improved sensitivity and precision for a wide m/z range of product ions

• Low noise level at 20 kV

- Improved signal to noise

Gain in Signal with Higher HED Voltage: Anti-EHR2/neu mAB

Peptides

60 – 120% increase in

response to 20kV vs. 10KV Product Ion (m/z)

Resp

onse

Incre

ase

(R

ela

tive

to 1

0K

V) Gain in Signal with Higher HED Voltage:

Anti-HER2/neu mAB Peptides

6495 QQQ - Premium Performance

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Improved sensitivity and lower LLOQs – Average 3x in S/N specifications and applications

Improved precision and excellent accuracy at the lowest levels – 3x in IDL specifications

Proven 6 orders of linear dynamic range

Proven robustness in complex matrix – Food matrix and biological matrix (plasma)

Improved mass range, fast scan speed and MRM acquisition rate

Improved Sensitivity – Peak Area Response and S/N Reserpine (+) and Chloramphenicol (-), 1 pg on-column

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6495 QQQ

Avg. Area = 14,519

Avg. S/N = 393,000:1

6490 QQQ

Avg. Area = 5,581

Avg. S/N = 157,000:1

6495 QQQ

Avg. Area = 8,808

Avg. S/N = 315,000:1

6490 QQQ

Avg. Area = 3,318

Avg. S/N = 125,000:1

4 x10

0

0.05

0.1

0.15

0.2

0.25

0.3

0.35

0.4

0.45

0.5

0.55

0.6

0.65

0.7

0.75

0.8

0.85

0.9

0.95

1

Counts vs. Acquisition Time (min)

0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 2.2 2.4 2.6 2.8

3 x10

0

0.25

0.5

0.75

1

1.25

1.5

1.75

2

2.25

2.5

2.75

3

3.25

3.5

3.75

4

4.25

4.5

4.75

5

5.25

5.5


0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 2.2 2.4 2.6 2.8 3 3.2 3.4 3.6

1 pg reserpine (+) (n=10) 1 pg chloramphenicol (-) (n=10)

2.6 x higher area response

2.5x higher S/N

2.6 x higher area response

2.5x higher S/N

• The 6495 QQQ LC/MS system shows improved peak area response and S/N, in both positive

and negative ion modes compared to previous designs

Many Ways to Manipulate S/N

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Variation in S/N measurements makes direct assessment difficult

Increase signal

• Increase the gain

• Narrow chromatographic peak width

• Increase scan averaging

Lower noise

• Select noise region

• Narrow the width of noise region

• Adjust baseline

• Apply peak smoothing & noise filtering

• Vary noise calculation algorithms: Peak-to-Peak, RMS, and ...

a. Peak-to-peak noise

b. RMS noise

c. Smoothing

d. Baseline noise filtering

Instrument Detection Limit (IDL) is Defined by Statistics

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IDLLCMS = t x SD = t x (%RSD / 100) x amount measured

Based on a well-established statistical formula, follows regulatory guidelines

• The minimum amount of analyte that is detectable and distinguishable from background noise with a confidence level

IDL

• Student “t” value, for

• 99% confidence level

• n – 1 degree of freedom t

• Relative standard deviation / precision of peak area at the amount measured

• From n replicate injections %RSD

• Limited to 2 – 5 x times higher than the Detection Limit (DL)

Amount measured

• Theoretical fitting of %RSD is based on

ion statistics

• %RSD increases at lower injected

amount

%RSD vs. Injected Amount

Why Add IDL Specs for QQQ LC/MS?

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S/N

Many factors impact S/N: - Lower noise - Increase signal

Significant variation in S/N measurements

A relatively high level is used for S/N measurement

Fails to estimate the true limits of detection and quantitation (LLOQ)

Not a good metric of sensitivity performance

IDL (%RSD)

Access sensitivity performance from area %RSD (precision) of replicate injections

Based on a well established statistical formula – follows IUPAC / EPA guidelines

An analytical low level is used for IDL measurement – determined using calibration curve

Accurate assessment of the true limits of detection and quantitation (LLOQ)

A better and more rigorous sensitivity performance metric

Improved Sensitivity and Precision – Peak Area %RSD Reserpine (+) and Chloramphenicol (-) at low levels, 5 fg on-column

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6495 QQQ

Avg. Area = 157

Area %RSD = 6.1

6490 QQQ

Avg. Area = 47

Area %RSD = 10.4

6495 QQQ

Avg. Area = 121

Area %RSD = 2.7

6490 QQQ

Avg. Area = 40

Area %RSD = 5.6

5 fg reserpine (+) (n=10) 5 fg chloramphenicol (-) (n=10)

3 x higher area response

Lower area %RSD

3 x higher area response

Lower area %RSD

• The improved sensitivity of the 6495 QQQ LC/MS system results in enhanced peak area

response and improved area precision (%RSD), especially at the low levels

• This ultimately leads to lower instrument detection limits (IDLs) compared to previous designs

2 x10

0.35

0.4

0.45

0.5

0.55

0.6

0.65

0.7

0.75

0.8

0.85

0.9

0.95

1

1.05

1.1

1.15

1.2

1.25

1.3

1.35

Counts vs. Acquisition Time (min) 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9

2 x10

0.35

0.4

0.45

0.5

0.55

0.6

0.65

0.7

0.75

0.8

0.85

0.9

0.95

1

1.05

1.1

1.15 1

Counts vs. Acquisition Time (min) 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 2.2 2.4 2.6 2.8 3 3.2 3.4 3.6

Improved Sensitivity and Precision – 6495 QQQ IDL

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1 fg of reserpine used to measure IDL (+) 1 fg of chloramphenicol used to measure IDL (-)

IDL = t x (%RSD / 100) x Amount measured

= 2.821 x (7.2 / 100) x 1 fg

= 0.20 fg

IDL = t x (%RSD / 100) x Amount measured

= 2.821 x (9.7 / 100) x 1 fg

= 0.27 fg

6495 QQQ IDL Amount measured Replicates Area %RSD t (99%) IDL

Reserpine (+) 1 fg n = 10 7.2 2.821 0.20 fg

Chloramphenicol (-) 1 fg n = 10 9.7 2.821 0.27 fg

5 fg

5 fg

1.02 fg

1.25 fg

7.2

8.9

Improved precision (%RSD) and Instrument Detection Limit (IDL) achieved on the 6495 vs. the 6490

IDL for the Agilent 6495 QQQ LC/MS System

1 x10

5.4

5.6

5.8

6

6.2

6.4

6.6

6.8

7

7.2

7.4

7.6

7.8

3.6 3.7 3.8 3.9 4 4.1 4.2 4.3

… 1 x10

5.4

5.6

5.8

6

6.2

6.4

6.6

6.8

7

7.2

7.4

7.6

7.8

Acquisition Time (min) 1.45 1.55 1.65 1.75 1.85 1.95 2.05

Acquisition Time (min)

6495 IDL Levels: Accurate Assessment of the LLOQs Reserpine (+) and Chloramphenicol (-)

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6495 QQQ - Reserpine (+) IDL 6495 QQQ – Chloramphenicol (-) IDL

0.20 fg 0.27 fg

6495 QQQ: Reserpine LLOQ = 0.2 fg 6495 QQQ: Chloramphenicol LLOQ = 0.3 fg

Blank Blank

Area % RSD = 15.8

n = 10 injections

%Accuracy = 118

Area % RSD = 18.8

n = 10 injections

%Accuracy = 108

• Improved / Lower IDL levels and LLOQ levels are achieved on the new 6495 vs. the 6490

1 x10

5.3

5.4

5.5

5.6

5.7

5.8

5.9

6

3.6 3.7 3.8 3.9 4 4.1 4.2 4.3

1 x10

5.4

5.5

5.6

5.7

5.8

5.9

6

6.1

6.2

6.3

1.45 1.55 1.65 1.75 1.85 1.95 2.05

1.02 fg 1.25 fg

6490 QQQ

Chloramphenicol LLOQ = 1.0 fg

6490 QQQ

Reserpine LLOQ = 1.0 fg

6495 QQQ LC/MS Performance Specifications

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Bid Specifications Agilent

6490

Agilent

6495

Sensitivity, S/N

Positive, 1 pg Reserpine on-column 50,000:1 150,000:1

Sensitivity, S/N

Negative, 1 pg chloramphenicol on-column 50,000:1 150,000:1

Sensitivity, Instrument Detection Limit (IDL)

Positive, Reserpine 2.5 fg

Measured at 5 fg on-column

0.75 fg Measured at 1 fg on-column

Sensitivity, Instrument Detection Limit (IDL)

Negative, chloramphenicol 2.5 fg

Measured at 5 fg on-column

0.75 fg Measured at 1 fg on-column

Linear dynamic range 106 106

Mass range 5 – 1,400 Da 5 – 2,250 Da

Maximum scan speed 12,500 Da/sec 15,000 Da/sec

Maximum MRM rate 250

MRMs/sec

500

MRMs/sec

Minimum MRM dwell time 1 ms 1 ms

Polarity switching 30 ms 30 ms

• Improved sensitivity specs: - S/N - IDL

• Improved mass range

• Improved full scan and MRM speed

Verapamil (+) Sensitivity in Solvent Attogram / Zeptomole Lower Limit of Quantitation

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• Unparalleled sensitivity (LLOQ of 40 ag on-column) - better than the 6490 (100 ag)

• Excellent precision (peak area %RSD) and accuracy are observed at the lowest levels

6495 QQQ: Verapamil LLOQ = 40 ag (80 zeptomoles) on-column

Blank

Area %RSD = 15.1

n = 10 injections

%Accuracy = 116

Verapamil 100 ag on-column

Improved LLOQ, 6495 vs. 6490

6495 QQQ

Area %RSD = 11.1

6490 QQQ

Area %RSD = 19.5

//upload.wikimedia.org/wikipedia/commons/0/01/Verapamil_Structural_Formulae.png

Verapamil IDL – Measured at the LLOQ Level

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Amount measured Replicates %RSD t (99%) IDL

40 ag (LLOQ) n = 10 15.1 2.182 17 ag

IDL = t x (%RSD/100) x Amount = 2.182 x (15.1/100) x 1.0 fg = 17 ag

Inj # Peak Area

1 16

2 19

3 19

4 20

5 19

6 13

7 18

8 21

9 16

10 14

%RSD 15.1

• Excellent peak area precision (%RSD) are observed at the lowest level (LLOQ)

1 x10

5.5

5.6

5.7

5.8

5.9

6

6.1

6.2

6.3

6.4

6.5

6.6

6.7

6.8

6.9

3.827 16

1 x10

5.5

5.6

5.7

5.8

5.9

6

6.1

6.2

6.3

6.4

6.5

6.6

6.7

6.8

6.9

7 3.824 19

1 x10

5.4

5.5

5.6

5.7

5.8

5.9

6

6.1

6.2

6.3

6.4

6.5

6.6

6.7

6.8

6.9 3.824

19


3.65 3.7 3.75 3.8 3.85 3.9 3.95 4

1 x10

5.6

5.8

6

6.2

6.4

6.6

6.8

7

7.2 3.820 20

1 x10

5.6

5.8

6

6.2

6.4

6.6

6.8

7

7.2 3.827 19

1 x10

5.5

5.6

5.7

5.8

5.9

6

6.1

6.2

6.3

6.4

6.5

6.6

6.7

6.8

6.9

7

3.820

13

3.65 3.7 3.75 3.8 3.85 3.9 3.95 4

1 x10

5.5

5.6

5.7

5.8

5.9

6

6.1

6.2

6.3

6.4

6.5

6.6

6.7

6.8 3.824

18

1 x10

5.5

5.6

5.7

5.8

5.9

6

6.1

6.2

6.3

6.4

6.5

6.6

6.7

6.8

6.9

7 3.824 21

1 x10

5.6

5.8

6

6.2

6.4

6.6

6.8

7 3.824

16

3.65 3.7 3.75 3.8 3.85 3.9 3.95

Acquisition Time (min) Acquisition Time (min)

1 x10

5.5

5.6

5.7

5.8

5.9

6

6.1

6.2

6.3

6.4

6.5

6.6

6.7

6.8

6.9 3.824 14

3.6 3.65 3.7 3.75 3.8 3.85 3.9 3.95 4


Inj 1

Inj 2

Inj 3

Inj 4

Inj 5

Inj 6

Inj 7

Inj 8

Inj 9

Inj 10

Bentazon (-) Sensitivity in Solvent Sub-femtogram Limits of Detection and Quantitation

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LLOQ = 0.5 pg/mL = 1.0 fg on-column in Solvent LOD = 0.2 pg/mL = 0.4 fg on-column in Solvent

• Unparalleled sensitivity (LLOQ 0.5 pg/mL and 1.0 fg on-column) – better than the 6490

• Excellent precision (peak area %RSD) and accuracy are observed at the lowest levels

Blank Blank

Injection volume = 2 µL

Area %RSD = 17.6

n = 7 injections

%Accuracy = 91.8

Avg. S/N = 5.9

Noise = Peak to Peak


Area %RSD = 12.4

n = 7 injections

%Accuracy = 85.1

Avg. S/N = 21.9


http://en.wikipedia.org/wiki/File:Bentazone_structure.svg

Bentazon IDL – Measured at the LLOQ Level

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0.5 pg/mL, 1.0 fg (LLOQ) n = 7 12.4 3.143 0.20 pg/mL, 0.39 fg

IDL = t x (%RSD/100) x Amount = 3.143 x (12.4/100) x 1.0 fg = 0.39 fg

Inj # Peak Area S/N (Peak to peak)

1 46 33.5

2 38 12

3 51 33.1

4 39 17.1

5 40 30.7

6 41 13.2

7 36 12.9

%RSD 12.4 Ave 21.9

Inj 1

Inj 2

Inj 3

Inj 4

Inj 5

Inj 6

Inj 7

• Excellent peak area precision (%RSD) are observed at the lowest level (LLOQ)


6 Orders of Linear Dynamic Range, Peptide Quantitation

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• Excellent assay accuracy and precision are

achieved at all levels including the LLOQ level

LVNEVTEFAK, 5 amol – 5 pmol, In trypsin digest

6 Orders of Linear Dynamic Range

R2 = 0.998

Levels %RSD

(n = 10)

%

Accuracy

RT %RSD

(n = 100)

5 amol 14.0 109.8

0.12

7.5 amol 16.0 108.7

15 amol 9.4 105.0

30 amol 9.0 87.1

300 fmol 1.6 85.2

3 fmol 1.2 81.4

30 fmol 0.6 86.4

300 fmol 0.7 87.4

3 pmol 2.1 105.6

5 pmol 10. 97.5

Zoom-in 5 – 30 amol

Proven System Robustness in Complex Food Matrix: Multi-Residue Pesticide Analysis in Black Tea

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• Pesticide standards analyzed before, in the middle, and after matrix injections

• No signal decrease was observed after 3 days of operation running with heavy food

matrix (black tea)

• %RSD < 6 (n = 6 data files) – Average results of > 250 pesticides analyzed

Overlaid six data files / MRM chromatograms • QC injections – Day 1

• QC injections – Day 2

• QC injections – Day 3

Unrivaled System Robustness in Biological Matrix: Protein Quantitation in Plasma

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• Selected peptides from 42 peptides in the QC sample – normalized to Day 1 response

• Peptide QC samples analyzed daily after every ~25 plasma digest injections

• No significant signal degradation observed after 853 injections of 40 µg plasma digest

per injection and 3.5 weeks of continuous operation

• Response %RSD: 6 - 15

0%

20%

40%

60%

80%

100%

120%

140%

01/06/14 01/11/14 01/16/14 01/21/14 01/26/14 01/31/14 02/05/14

Apolipoprotein E

L-selectin

Plasminogen

Albumin_serum

Kininogen

Transthyretin

Hemopexin

Day of Analysis

Resp

on

se

Ultra-Fast MRM Acquisition – 1ms Dwell Time

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Calibration Curve at 1ms dwell

1 – 1000 ng/mL

123 Pesticides, 1 ng/mL

• Fast MRM allows enough data

points across the peak for precise

and accurate multi-compound

quantitation with UHPLC speed

Carbosulfan, 18 data points

1,000 ng/mL

%Accuracy = 100.4

100 ng/mL

%RSD = 4.2

%Accuracy = 99.5 1 ng/mL

%RSD = 4.4

%Accuracy = 108.3

10 ng/mL

%RSD = 6.2

%Accuracy = 92.1

• Precision %RSD < 20

• %Accuracy 80 - 120

Improved Mass Range

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• Improved peptide quantitation can be achieved with the 6495

- Extended mass range up to 2,250 m/z

- Increased detection efficiency (HED voltage up to 20kV)

6495 QQQ MRM Spectrum for Glycopeptide:

EEQYN[+1606.6]STYR (G1F)

m/z 2227.88

6495 QQQ Key Applications

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Food Safety - Pesticides

Analysis of >250 trace level pesticides in food matrices (e.g. black tea) with extensive sample dilutions

Food Safety - Estrogens

Determination of ultra-trace level estrogens in milk product

Environmental - Water Analysis

Quantitation of ultra-trace level hormones (EDCs) in drinking water using direct injection

Peptide Quantitation

Quantitation of synthetic peptide at sub-attomole level using nanoflow and standard flow chromatography


Quantitative analysis of microcystins (cyclic nonribosomal peptides) in liver extract samples using stand flow chromatography

High Sensitivity Allows Extensive Sample Dilution

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1:20 dilution of > 250 pesticides spiked into black tea at 10 µg/kg (MRL), 0.1 ng/mL

1:100 dilution of pesticides in black tea at MRL, 20 pg/mL

• Multi-residue pesticide analysis in food products – most demanding food safety applications

• Improved sensitivity and precision of the 6495 allows accurate quantitation of pesticides

< Maximum Residue Limits (MRLs) imposed by EU, even with extensive sample dilution

• Sample dilution leads to less matrix effects and improved method robustness


Ultimate Sensitivity for Pesticides in Negative Mode Sub-pg/mL LLOQs and Sub-fg IDLs

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Pesticides

Fipronil

Bentazon

Teflubenzuron

Hexaflumuron

Flubendiamide

Fluazinam

Black Tea Matrix

LOD

(pg/mL)

LLOQ

(pg/mL)

IDL

pg/mL fg

1 1 0.39 0.78

0.5 1 0.36 0.72

5 5 2.13 4.25

5 5 1.09 3.19

1 5 0.54 1.08

5 5 2.13 4.25

Neat Solvent

LOD

(pg/mL)

LLOQ

(pg/mL)

IDL

pg/mL fg

0.2 0.5 0.14 0.27

0.2 0.5 0.20 0.39

0.5 1 0.37 0.73

0.5 1 0.38 0.75

0.5 1 0.27 0.54

0.5 1 1.10 2.20

Fipronil 1 pg/mL

Bentazon 0.5 pg/mL

Fluazinam 5 pg/mL

Flubendiamide 1 pg/mL

Hexaflumuron 5 pg/mL

Teflubenzuron 5 pg/mL

LOD Levels in Black Tea Matrix


• LLOQs < 20 pg/mL (40 fg on-column) is required to achieve MRL with 1:100 dilution

Bentazon Sensitivity in Black Tea Low femtogram Limits of Detection and Quantitation

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LLOQ = 1 pg/mL = 2.0 fg in 20x Diluted Black Tea LOD = 0.5 pg/mL = 1.0 fg in 20x Diluted Black Tea

• Unparalleled sensitivity in black tea matrix - LLOQ 1.0 pg/mL and 2.0 fg on-column

• Excellent precision (peak area %RSD) and accuracy are observed at the low levels in

black tea matrix, e.g. the LLOQ level.


Area %RSD = 16.9

n = 7 injections

Avg. S/N = 7.4


95.79


Area %RSD = 11.4

n = 7 injections

%Accuracy = 109.8

Avg. S/N = 16.1


http://en.wikipedia.org/wiki/File:Bentazone_structure.svg

Bentazon: Superb Precision & IDL in Black Tea

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31


1 pg/mL, 2.0 fg (LLOQ) n = 7 injections 11.4 3.143 0.36 pg/mL, 0.72 fg

IDL = t x (%RSD/100) x Amount = 3.143 x (11.4/100) x 2.0 fg = 0.72 fg

Inj # Peak

Area

S/N (Peak to peak)

1 82 20.9

2 96 13.2

3 70 12.7

4 86 13.4

5 92 10.3

6 72 12.7

7 83 29.4

%RSD 11.4 Ave 16.1


• Excellent peak area precision (%RSD) are observed at the lowest level in black tea matrix

1 pg/mL

1 pg/mL

1 pg/mL

1 pg/mL

1 pg/mL

1 pg/mL

1 pg/mL

Five Orders of Linear Dynamic Range

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32

Zoom-in 1 – 100 pg/mL

• Wide (5 orders) linear dynamic range, excellent assay accuracy and precision at all levels

Levels %RSD (n = 7) %Accuracy

1 pg/mL 10.41 82.4

5 pg/mL 5.84 87.2

10 pg/mL 6.41 82.6

50 pg/mL 6.10 97.4

100 pg/mL 3.86 100.8

500 pg/mL 1.73 108.8

1 ng/mL 2.93 112.5

5 ng/mL 2.25 119.7

10 ng/mL 2.54 116.8

50 ng/mL 2.62 102.3

100 ng/mL 2.51 95.8

Fipronil (-) in 1:20 diluted black tea

1 pg/mL – 100 ng/mL (2 fg - 200 pg)

5 Orders

R2 = 0.995

Spinosyn A (+) in 1:20 diluted black tea

1 pg/mL – 100 ng/mL (2 fg - 200 pg)

5 Orders

R2 = 0.997 Log – Log scale

Levels %RSD (n = 7) %Accuracy

1 pg/mL 15.0 110.7

5 pg/mL 10.9 85.7

10 pg/mL 6.8 97.2

50 pg/mL 3.6 86.0

100 pg/mL 5.4 100.5

500 pg/mL 7.3 96.3

1 ng/mL 6.5 105.0

5 ng/mL 2.1 105.8

10 ng/mL 3.6 111.3

100 ng/mL 1.2 96.2

Signal Improvements for High Relevance Pesticides

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33

6495 6490 6495 6490

Methidathion

Area Gain 3.7x

Methomyl

Area Gain 3.5x

Buprofezine

Area Gain 4.2x

Monocrotophos

Area Gain 2.9x

Thiabendazole

Area Gain 3.2x

Spiromesifen

Area Gain 2.1x

6495 6490 6495 6490

6495 6490 6495 6490

• Improved sensitivity (peak area gain of 3x) are observed on the new 6495 vs. the 6490

Improved Precision & Lower LLOQs in Black Tea

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34

• Improved precision (%RSD) are observed, particularly at the lowest levels (LLOQs)

• The enhanced peak area response and improved precision (%RSD) means pesticides

achieve lower LLOQs ( MRL level) using the new 6495.

Comparison of Area %RSD for 50 Pesticides at MRL # of pesticides detected at MRL

• 67% of pesticides were easily detected

with a %RSD < 20 with 1:100 dilution

• This means LLOQs < 20 pg/mL (ppt)

• %RSD < 20 meets SANCO guidelines

6495

Dilution allows More Efficient Ionization of Pesticides

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35

1 : 5 1 : 10 1 : 20 1 : 100 1 : 50

1 : 5 1 : 10 1 : 20 1 : 100 1 : 50

Alanycarb, 10 µg/kg in Black Tea

No

Dilution

Oxamyl, 10 µg/kg in Black Tea

No

Dilution

Recovery (%) in Black Tea with Different Dilutions

7/31/2014


36

Pesticides No dilution

(n = 5)

Dilution 1:5

(n = 5)

Dilution 1:10

(n = 5)

Dilution 1:20

(n = 5)

Dilution 1:50

(n = 5)

Dilution 1:100

(n = 5)

Acetamiprid 29.4 ± 0.8 57.3 ± 1.4 67.5 ± 3.7 79.9 ± 2.9 91.8 ± 5.2 109.5 ± 3.4

Alanycarb 10.4 ± 1.3 73.9 ± 2.2 81.5 ± 14.3 85.7 ± 11.1 87.6 ± 4.7 121.7 ± 10.8

Aldicarb 36.9 ± 1.0 69.9 ± 1.4 78.0 ± 3.5 91.0 ± 4.2 95.2 ± 8.8 104.9 ± 14.1

Carbaryl 56.9 ± 1.8 80.1 ± 3.8 80.8 ± 4.1 96.1 ± 7.2 102.6 ± 6.6 116.4 ± 9.6

Dimethoate 33.9 ± 1.7 68.6 ± 2.4 84.1 ± 5.4 89.0 ± 7.9 88.2 ± 8.8 84.7 ± 7.5

Diuron 79.7 ± 4.0 90.4 ± 7.0 91.7 ± 4.9 94.9 ± 7.2 89.2 ± 7.3 100.9 ± 13.5

Flufenoxuron 95.4 ± 1.1 88.8 ± 1.6 89.4 ± 3.8 93.3 ± 5.8 100.0 ± 6.1 119.2 ± 13.9

Monocrotophos 4.6 ± 0.3 13.9 ± 0.3 21.8 ± 0.8 33.8 ± 1.1 58.5 ± 2.0 95.1 ± 5.7

Oxamyl 20.8 ± 0.7 52.6 ± 1.9 65.0 ± 2.0 79.7 ± 3.0 91.2 ± 4.6 110.6 ± 5.2

Thiamethoxam 40.0 ± 1.4 45.9 ± 0.9 46.6 ± 3.8 52.2 ± 1.7 70.9 ± 2.9 97.3 ± 2.0

• Cells shaded in green shows %recovery of 80 – 120, which are in compliance with

SANCO requirements.

• Pesticides show full recovery and no signal suppression with 1:100 dilution

• Neat solvent calibration curve can be used for pesticides quantitation with 1:100 dilution


7/31/2014


37











Sensitive Quantitation of Estrogens in Cow Milk

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38

Eight estrogens spiked in cow milk at 0.1 µg/kg, corresponding to 0.1 ng/mL

• Estrogen levels in milk products are regulated by government agencies down to 1 µg/kg

• Highly sensitive and precise quantitation of estrogens was achieved using the new 6495

with a simple QuEChERS procedure without derivatization or enrichment.

• LLOQs for all estrogens (<< 0.1 µg/kg) are far below the regulatory requirement (1 µg/kg)

ID Compounds

1 Estriol (-)

2 17-α-Estradiol (-)

3 17-β-Estradiol (-)

4 Ethynyl estradiol (-)

5 Estrone (-)

6 Diethylstilbestrol (-)

7 Hexestrol (-)

8 Dienestrol (-)

2 3

4

5

6

7,8

1

--- 0.1 ng/mL estrogens in cow milk

--- Milk blank


https://www.google.com/url?q=http://www.anh-usa.org/raw-milk-is-good-milk/&sa=U&ei=elMfU9uyDsPekgXuh4HADQ&ved=0CEEQ9QEwCg&sig2=SPEt5_PRKwiNck_2cwiqZQ&usg=AFQjCNErSA0Z2qVskftnULA3Ki3du8Gq7w

Highest Sensitivity and Excellent Precision LLOQs and IDLs at pg/mL (fg) level

Compounds LLOQ

(pg/mL)

%RSD

n = 7

IDL

pg/mL fg

Estriol 5 11.3 1.8 8.9

17-α-Estradiol 10 8.4 2.7 13.3

17-β-Estradiol 10 11.4 3.6 18.0

Ethynyl estradiol 10 11.7 3.7 18.5

Estrone 5 9.6 1.5 7.5

Diethylstilbestrol 10 12.8 2.0 10.1

Hexestrol 5 11.1 1.7 8.7

Dienestrol 5 7.6 1.2 6.0

Estriol

5 pg/mL

17-Estradiol

10 pg/mL

17-Estradiol

10 pg/mL

Ethynyl Estradiol

10 pg/mL

Estrone

5 pg/mL

Diethylstilbestrol

10 pg/mL

Hexestrol

5 pg/mL

Dienestrol

5 pg/mL

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39




Estriol: Superb Precision and IDL at Low-fg Level

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40

Estriol Amount measured Replicates %RSD t (99%) Estriol IDL

5 pg/mL, 25 fg (LLOQ) n = 7 injections 11.3 3.143 1.8 pg/mL, 8.9 fg

IDL = t x (%RSD/100) x Amount = 3.143 x (10.4/100) x 25 fg = 8.9 fg

Inj # Peak

Area S/N

(Peak to peak)

1 44 11.1

2 54 11.1

3 41 9.8

4 46 9.8

5 50 10.5

6 54 8.6

7 52 9.9

%RSD 11.3 Ave. 10.1


5 pg/mL

5 pg/mL

5 pg/mL

5 pg/mL

5 pg/mL 5 pg/mL

5 pg/mL

• Excellent peak area precision (%RSD) are observed at the lowest levels (LLOQs)


Confident Estrogen Quantitation in Milk

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41

Levels (µg/kg) %RSD (n = 7) %Accuracy

0.1 5.33 106.0

0.2 2.96 101.2

0.5 2.99 94.6

1 2.75 95.3

2 0.94 100.3

5 1.68 104.1

10 1.64 98.5

Levels (µg/kg) %RSD (n = 7) %Accuracy

0.1 3.86 113.8

0.2 2.61 102.5

0.5 3.68 91.1

1 1.74 92.5

2 1.45 97.9

5 3.64 101.6

10 2.14 100.6

• Excellent linearity (R2 ≥0.998), accuracy and precision are achieved at all levels

17 -Estradiol in milk, 0.1 – 10 µg/kg

0.1 – 10 ng/mL

R2 = 0.999

Estriol in milk, 0.1 – 10 µg/kg

0.1 – 10 ng/mL

R2 = 0.999


Summary of Estrogen Quantitation in Milk Excellent linearity, precision, accuracy and matrix recovery

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Compounds R2 Precision

%RSD, n = 7 %Accuracy

%Recovery

0.2 µg/kg 1 µg/kg 5 µg/kg

Estriol 0.9988 0.94 – 5.33 94.6 – 106.0 92.9 102.8 85.8

17-α-Estradiol 0.9973 1.32 – 5.31 90.6 – 112.0 92.2 99.4 93.4

17-β-Estradiol 0.9985 1.45 – 3.86 91.1 – 113.8 92.9 106.0 96.9

Ethynyl estradiol 0.9984 1.59 – 6.61 90.1 – 114.5 90.2 104.9 94.0

Estrone 0.9987 1.45 – 3.76 92.4 – 110.7 94.0 101.7 96.3

Diethylstilbestrol 0.9985 2.27 – 5.04 94.9 – 104.6 83.5 98.6 91.0

Hexestrol 0.9981 0.88 – 4.58 90.1 – 112.8 86.1 98.4 91.8

Dienestrol 0.9995 1.24 – 4.34 95.6 – 102.7 82.9 92.8 85.3



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Analysis of Hormones in Drinking Water

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44

• Endocrine Disrupting Chemical (EDCs) levels in

municipal water supplies are regulated by government

agencies down to ng/L (ppt) level

• The highly sensitive 6495 allows the quantitation of

EDCs at sub ng/L using direct injection

• No need for time consuming offline SPE or enrichment

Estriol 17-β-Estradiol

Testosterone

17-α-Ethynylestradiol Equilin Estrone

Androstenedione

Estrone (-)

Estriol (-)

Testosterone (+)

Androstenedione (+)

Equilin (-)

17--Ethynyl estradiol (-)

17--estradiol (-)

Hormones in drinking water, 5 ng/L (testosterone) to 17.5 ng/L (EE)

http://en.wikipedia.org/wiki/File:Estradiol.svg

http://en.wikipedia.org/wiki/File:Estriol.svg

http://en.wikipedia.org/wiki/File:Estron.svg

http://en.wikipedia.org/wiki/File:Ethinylestradiol-2D-skeletal.svg

Highest Sensitivity and Excellent Precision LLOQs and IDLs at sub-ng/L Level

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45

Estriol

2 ng/L

Testosterone

0.1 ng/L

Equilin

0.2 ng/L

17-

Ethynylestradiol

1.75 ng/L

17β-Estradiol

0.5 ng/L

Estrone

0.2 ng/L

Androstenedione

0.2 ng/L

Compounds LLOQ

(ng/L)

%RSD

n = 8

IDL

(ng/L)

%

Accuracy

Estriol 1 5.6 0.168 94.8

17β-Estradiol < 0.5 13.5 0.202 102.2

Testosterone 0.1 10.3 0.031 114.5

17α-Ethynylestradiol 1.75 14.8 0.78 98.4

Equilin < 0.2 3.7 0.022 115.1

Estrone < 0.2 7.2 0.043 115.1

Androstenedione < 0.2 4.3 0.026 107.2

17-Estradiol: Superb Precision and Ultra-Low IDL

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46

17-Estradiol Measured Replicates %RSD t (99%) IDL

0.5 ng/L (LLOQ) n = 8 injections 13.5 2.998 0.202 ng/L

MDL = t x (%RSD/100) x Amount = 2.998 x (13.5/100) x 0.5 ng/L = 0.202 ng/L

Inj # Peak

Area

S/N (Peak to peak)

1 869 9.1

2 925 10.0

3 671 6.7

4 699 6.8

5 950 11.5

6 946 16.0

7 857 8.2

8 762 12.2

%RSD 13.5 10.1

0.5 ng/L

0.5 ng/L

0.5 ng/L

0.5 ng/L

0.5 ng/L 0.5 ng/L

0.5 ng/L

0.5 ng/L


Confident Quantitation of EDCs in Drinking Water

7/31/2014


47

• Excellent linearity (R2 ≥0.995), assay accuracy and precision are achieved at all levels

Testosterone (+)

0.1 – 10 ng/L

R2 = 0.996

17-estradiol (-)

0.1 – 10 ng/L

R2 = 0.996

Levels (ng/L) %RSD (n = 8) %Accuracy

0.1 10.3 114.5

0.2 4.89 104.2

0.5 2.71 88.8

1 4.34 92.8

5 4.49 97.4

10 2.09 104.7

Levels (ng/L) %RSD (n = 8) %Accuracy

0.1 13.7 120.3

0.2 13.5 102.2

0.5 3.78 87.5

1 4.14 89.1

5 2.66 98.8

10 0.73 104.2

Summary of Hormone Quantitation in Drinking Water Excellent linearity, precision and accuracy at all levels

7/31/2014


48

Compounds LLOQ

(ng/L)

IDL

(ng/L) R2 Precision

(%RSD, n = 8) %Accuracy

Estriol 0.4 0.168 0.997 0.81 – 10.6 93.6 – 113.6

17β-Estradiol < 0.5 0.202 0.996 0.73 – 13.7 87.5 – 120.3

Testosterone < 0.1 0.031 0.994 2.09 – 10.3 88.8 – 114.5

17α-Ethynylestradiol 1.75 0.78 0.996 2.50 – 16.5 95.3 – 107.2

Equilin < 0.2 0.022 0.997 3.00 – 5.21 88.9 – 115.1

Estrone < 0.2 0.043 0.996 2.57 – 9.41 91.2 – 115.1

Androstenedione < 0.1 0.026 0.995 1.48 – 9.07 89.2 – 107.2


7/31/2014


49











Outstanding Sensitivity with Standard Flow LC

7/31/2014


50

Levels %RSD

(n = 10)

%

Accuracy

RT %RSD

(n = 100)

5 amol 14.0 109.8

0.12

7.5 amol 16.0 108.7

15 amol 9.4 105.0

30 amol 9.0 87.1

300 fmol 1.6 85.2

3 fmol 1.2 81.4

30 fmol 0.6 86.4

300 fmol 0.7 87.4

3 pmol 2.1 105.6

5 pmol 10. 97.5

Zoom-in 5 – 30 amol

LVNEVTEFAK

5 amol – 5 pmol

6 Orders

R2 = 0.998

• Low attomole sensitivity for synthetic peptide spiked in tryptic

digest using the 6495 with standard flow chromatography

• Six orders of linear dynamic range

• Excellent precision and accuracy are observed at all levels

including the LLOQ level

Blank

3 amol

5 amol

Area %RSD = 14.0, n = 10

%Accuracy = 109.8

7.5 amol

LOD

LLOQ


Excellent Precision and Low Attomole Level IDL

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51


5.0 amol (LLOQ) n = 10 injections 14.0 2.821 2.0 amol

MDL = t x (%RSD/100) x Amount = 2.821 x (14.0/100) x 5.0 amol = 2.0 amol

Injection # Peak Area

1 30.5

2 28.7

3 30.4

4 33.91

5 35.1

6 31.5

7 34.5

8 20.7

9 26.7

10 26.6

%RSD 14.0

5 amol

5 amol

5 amol

5 amol

5 amol

5 amol

5 amol

5 amol

5 amol

5 amol


Ultimate Sensitivity with Nanoflow LC

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52

• Ultimate sub-attomole sensitivity for synthetic peptide spiked

in tryptic digest using the 6495 with nanoflow chromatography

• Wide (over 5 orders) linear dynamic range

• Excellent precision and accuracy are observed at all levels

including the LLOQ level

LVNEVTEFAK

0.5 amol – 0.1 pmol

> 5 Orders

R2 = 0.99996

Levels (amol) %RSD %Accuracy

0.5 4.52 103.2

1 8.50 80.2

10 5.75 84.6

100 4.71 88.9

1000 1.23 85.6

10,000 1.09 97.6

100,000 1.69 100.4


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Analysis of Microcystins

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54

• Microcystins are cyanotoxins that may cause serious damage to the liver of mammals

• Microcystins are challenging compounds to analyze due to the low levels in biological matrices

MRM chromatograms of microcystins

Using the 6495 with 1290 UHPLC

Outstanding Sensitivity and Linear Dynamic Range Excellent Peak Area Precision %RSD at the LLOQ Levels (pg/mL)

7/31/2014


55

Concentration (ng/ml)

0.1 1 10 100

R e

s p

o n s e

s

6 x10

5E-05

0.0001

0.0005

0.001

0.005

0.01

0.05

0.1

0.5

1

R^2 = 0.99495440

Type:Linear, Origin:Ignore, Weight:1/x^2

RR: 2.5-50,000 ng/mL > 4 Orders of Linear Dynamic Range R2 = 0.995

1 x10

4.5

5

5.5

6

6.5

7

7.5

1 x10

4.5

5

5.5

6

6.5

7

7.5 2.297 78.51


1.8 1.9 2 2.1 2.2 2.3 2.4 2.5 2.6

Blank

Concentration (ng/ml)

0.05 0.1 0.5 1 5 10 50 100

R e

s p

o n s e

s

4 x10

0.006

0.008

0.01

0.02

0.04

0.06

0.08

0.1

0.2

0.4

0.6

0.8

1

2

4 R^2 = 0.99752628

Type:Linear, Origin:Ignore, Weight:1/x

LY: 50-50,000 ng/mL 3 Orders of Linear Dynamic Range R2 = 0.998

1 x10

4.25

4.5

4.75

5

5.25

5.5

5.75

6

6.25

6.5

1 x10

4.25

4.5

4.75

5

5.25

5.5

5.75

6

6.25

6.5 5.895 56.82


5.2 5.4 5.6 5.8 6 6.2

LY, LLOQ 50 pg/mL %RSD = 10.3 n = 3

Blank

• Excellent linearity (R2 ≥ 0.99),

assay accuracy and precision

are achieved for all seven

microcystins and at all levels

RR, LLOQ 2.5 pg/mL %RSD = 2.97 n = 3

Analysis of Microcystins in Liver Extract

7/31/2014


56

2 x10

0.6

0.8

1

1.2

1.4

1.6

1.8

2

2.2

6.7 6.8 6.9 7 7.1 7.2 7.3 7.4 7.5 7.6 7.7

2 x10

0.6

0.7

0.8

0.9

1

1.1

1.2

6.5 6.6 6.7 6.8 6.9 7 7.1 7.2 7.3 7.4 7.5

2 x10

0.5

0.6

0.7

0.8

0.9

1

1.1

1.2

1.3

1.4

5.3 5.4 5.5 5.6 5.7 5.8 5.9 6 6.1 6.2 6.3

2 x10

1.75

2

2.25

2.5

2.75

3

3.25

3.5

3.75

4

4.25

5.2 5.3 5.4 5.5 5.6 5.7 5.8 5.9

2 x10

0.5

0.75

1

1.25

1.5

1.75

2

2.25

2.5

2.75

3 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9

2 x10

1

2

3

4

5

6

7

2.1 2.2 2.3 2.4 2.5 2.6

Retention time (min)

RR 930 pg/mL

YR 107 pg/mL

LR 155 pg/mL

LY 292 pg/mL

LW 176 pg/mL

LF 195 pg/mL

LA 179 pg/mL 2

x10

0.5

0.6

0.7

0.8

0.9

1

1.1

1.2

1.3

1.4

3 3.1 3.2 3.3 3.4 3.5 3.6 3.7

• The highly sensitive 6495 allows confident quantitation of microcystins at pg/mL levels in liver

extract samples

Summary of Microcystins Quantitation Excellent linearity, precision and accuracy at all levels

7/31/2014


57

Compounds LLOQ

(pg/mL)

Range

(pg/mL) R2 Precision

(%RSD, n = 3) %Accuracy

Liver Extract

(pg/mL)

RR 2.5 2.5 - 50,000 0.9949 0.13 - 6.24 93.9 - 111.7 930

YR 2.5 2.5 - 50,000 0.9906 1.30 - 13.51 91.6 - 111.9 107

LR 2.5 2.5 - 50,000 0.9921 0.41 - 11.35 92.2 - 107.5 155

LA 25 25 - 50,000 0.9984 0.57 - 5.40 85.8 - 101.9 179

LY 50 50 - 50,000 0.9975 2.80 – 1034 88.8 - 119.4 292

LW 25 25 - 50,000 0.9987 0.66 - 14.3 85.6 - 105.1 176

LF 25 25 - 50,000 0.9975 1.62 - 6.35 85.5 - 115.0 195

MassHunter Acquisition B.07

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58

Acquisition

Quant

Support for 6495 QQQ LC/MS

Faster and accurate autotune

Improved workflows

Optimizer to better support tMRM

Dynamic MRM (dMRM) and triggered MRM (tMRM)

iFunnel express, Study manager, Skyline automation, application kits and solutions

Fast and Accurate Automatic Tuning Improved System Robustness

7/31/2014


59

• New autotune reduces the time by 40 min (40%) when both POS & NEG polarities are tuned

• New checktune reduces the time by 2 min (25%) when both polarities are checktuned

• Enhanced quadrupole optics tuning algorithms results in higher ion transmission

Shortens 20 min

for each polarity

Dynamic MRM (dMRM)

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60

Dynamic MRM, Fast MRM Speed and Polarity Switch

• Group MRMs in RT windows

instead of time segments

• 2x shorter cycle time supports

narrow UHPLC peaks

• Supported by very fast MRM

speed (1 ms dwell) and

polarity switch (20 ms)

Triggered MRM (tMRM)

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61

• tMRM database accelerates

acquisition method setup

• tMRM library – Match Score

for confirmation

• tMRM is Fast and Sensitive

• UHPLC compatible

• Simultaneous Quantitation,

Screening and Confirmation

Triggered MRM, tMRM Database and Library

Match

Score

95.1 Trigger

Threshold

tMRM Product

Ion Spectrum

Apex

Enhanced Optimizer Workflow in MassHunter B.07 (to Better Support tMRM)

• Find 10 MRM transitions in Optimizer

• Injection volume flexibility

• Improved method editing

MassHunter

Optimizer B.07

iFunnel Express iFunnel Express – Source and iFunnel Optimization

Method Generation Acquisition Optimization

• Automate the source/iFunnel

optimization process

Create

Methods

& Worklist

MH Source

and Funnel

Optimizer

Acquire Data

for Each

Parameter

MassHunter

Quant

Data

Files Integrate for

Peak Data

Excel File for

Optimized

Parameter

7/31/2014


62

Study Manager and Skyline Automation

Skyline Automation

Study Manager – Fully Automated Quantitation Workflow

1. Specify input File 2. Specify Quant Method 3. Specify Report 4. Submit and go!

MH Acquisition MH Quant

• Automated workflows for peptide quantitation

7/31/2014


63

LC/MS Applicatio

n Kits

Method

Standards

On Site Trainin

g

LC Column

tMRM Databas

e & Library

tMRM Application Kits For LC/MS Targeted Screening & Confirmation with QQQ

Pesticides

Test Mix: 254 compounds

DB: 700+ compounds

Library: 200+ compounds

Veterinary Drugs


DB: 500+ compounds


Forensic Toxicology


DB: 2500+ compounds


• Agilent unique data dependent acquisition for fast and sensitive compound

screening, quantitation and confirmation.

7/31/2014


64

7/31/2014


65

Summary: Value of the New 6495 QQQ LC/MS

Increased sensitivity

Lowest Limits of Quantitation

Reproducible, High Quality Data

- More Confident Results

Ease of Operation & Fast Set-up

- Get Answers Quickly!

Screen, Confirm and Quantify

with UHPLC Speed

Streamlined Analytical Workflow

- More Productive Lab

Simpler Methods & Faster

Results

Less Maintenance & Downtime

Excellent precision & accuracy

at the Lowest Levels Wide linear dynamic range

Fast MRM speed, dynamic and

triggered MRM

Ease to use, workflow driven

software, kits and solutions

Proven robustness and

stability in complex matrices

Sample dilution

Direct injection, LC…

Confident quantitation and confirmation for the most demanding applications

http://www.google.com.hk/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&docid=EZkvPxsusF6U1M&tbnid=XrjI5Byc-fhyOM:&ved=0CAUQjRw&url=http://www.precisionmed.com/&ei=PaWFU5_qOYvYPOqIgaAL&psig=AFQjCNEr5vF01uTbPF-zi1Io3O7WCcax3A&ust=1401353870493589

6495 Brochure, Video, and Application Notes

7/31/2014


66

6495 Brochure: 5991-4541EN

6495 Video

6495 App Note: 5991-4687EN

6495 App Note: 5991-4686EN

6495 App Note: 5991-4685EN

ASMS 2014, Baltimore Celebrating Achievement

7/31/2014


67

agilent 6495 triple quadrupole lc/ms system 6495 triple quadrupole lc/ms system experience a new...

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