BioSolution
Agilent LC & LC/MS .
Agilent
.
2 Agilent LC & LC/MS
Small Molecules MW < 3000
Small Molecules MW < 3000
. 80~120 300 .
Large Molecules MW > 3000
Large Molecules MW > 3000
Eclipse Plus C18 300SB-C18
C18 . C18 , C18 , C8 . LC , Poroshell120 EC-C18 Eclipse Plus RRHT/RRHD (UHPLC ) .
Eclipse Plus C18 4.6 x 150 mm, 3.5μm 959963-902
ZORBAX 300SB-C18 4.6 x 150 mm, 5μm
883995-902
ZORBAX RRHD Eclipse Plus C18 2.1 x 50 mm, 1.8μm
959757-902
865973-902 Poroshell 300SB-C18 2.1 x 75 mm, 5μm
660750-902
Agilent



ZORBAX Eclipse Plus / XDB - -
Poroshell 120 - LC UHPLC -
Bonus-RP - Triple Endcapping Peak Tailing - C18 - 100% - SB
SB-Aq - pH - - -
Polaris - - -
RRHT / RRHD - - UHPLC
StableBond (SB) - pH - -
Extend C18 - pH(11.5) (pH 2-11.5) -
PLRP-S - pH 1-14 - “Polymer ” - Pore Size (100~4000)
Pursuit - pH 1~10 - Carbon Loading
Agilent
StableBond (SB) : pH 1~6
Extend-C18 : pH 2~11.5
Pursuit : pH 1.5~10 (Diphenyl pH 1.5~7.5)
Bonus-RP : pH 2~8
SB-Aq : pH 1~8
Polaris : pH 1.5~10
PLRP-S : pH 1~14
•
• QA/QC
• 1.8,3.5,5μm
•
Bonded Phase Pore Size
Endcapped Carbon Load
ZORBAX Eclipse Plus C18 95 160 m2/g 60 °C 2.0-9.0 Double 9%
ZORBAX Eclipse Plus C8 95 160 m2/g 60 °C 2.0-9.0 Double 7%
ZORBAX Eclipse PAH 95 160 m2/g 60 °C 2.0-8.0 No 14%
ZORBAX Eclipse Plus Phenyl-Hexyl 95 160 m2/g 60 °C 2.0-8.0 Double 9%
Bonded Phase Pore Size
Endcapped Carbon Load
ZORBAX Eclipse XDB-C18 80 180 m2/g 60 °C 2.0-9.0 Double 10%
ZORBAX Eclipse XDB-C8 80 180 m2/g 60 °C 2.0-9.0 Double 7.6%
ZORBAX Eclipse XDB-Phenyl 80 180 m2/g 60 °C 2.0-9.0 Double 7.2%
ZORBAX Eclipse XDB-CN 80 180 m2/g 60 °C 2.0-8.0 Double 4.3%
• pH 1 pH (80-90°C)

• SB-AQ 100%
• Peptide Protein SB300
•
• pH(pH 11.5)
• pH
• LC/MS Peptide
•

• Carbon Loading Capacity
•
R =Isopropyl, Isobutyl R1=C18, C8, CN, C3 etc.
• (eXtra Densely Bonded)

• (Capillary, ,
Prep )
Base Silica
1
2
ZORBAX SB-C18 80 180 m2/g 90 °C 0.8-8.0 No 10%
ZORBAX SB-C8 80 180 m2/g 80 °C 1.0-8.0 No 5.5%
ZORBAX SB-C3 80 180 m2/g 80 °C 1.0-8.0 No 4%
ZORBAX SB-Phenyl 80 180 m2/g 80 °C 1.0-8.0 No 5.5%
ZORBAX SB-CN 80 180 m2/g 80 °C 1.0-8.0 No 4%
ZORBAX SB-Aq 80 180 m2/g 80 °C 1.0-8.0 No proprietary
Bonded Phase Pore Size
ZORBAX Extend-C18 80 180 m2/g 60 °C 2.0-11.5 Double 12.5%
Bonded Phase Pore Size
Pore Volume
Ligand Coverage
Pursuit C18 200 200 m2/g 1.5-10 Yes 12.9% 11 mL/g 3.5 μmol/m2
Pursuit C8 200 200 m2/g 1.5-10 Yes 7.4% 11 mL/g 3.8 μmol/m2
Pursuit Diphenyl 200 200 m2/g 1.5-8.0 Yes 7.3% 11 mL/g 2.8 μmol/m2
Pursuit PFP 200 200 m2/g 1.5-10 Yes 6.3% 11 mL/g 3.4 μmol/m2
Pursuit PAH 200 200 m2/g 1.5-10 Yes 11 mL/g
Pursuit XRs C18 100 440 m2/g 1.5-10 Yes 22% 11 mL/g 2.9 μmol/m2
Pursuit XRs C8 100 440 m2/g 1.5-10 Yes 15% 11 mL/g 3.7 μmol/m2
Pursuit XRs Diphenyl 100 440 m2/g 1.5-8.0 Yes 14.6% 11 mL/g 2.6 μmol/m2
Pursuit XRs Si 100 440 m2/g 1.5-10 Yes 11 mL/g
Pursuit XRs Ultra C18 100 440 m2/g 1.5-10 Yes 23.2% 11 mL/g 3.2 μmol/m2
Pursuit XRs Ultra C8 100 440 m2/g 1.5-10 Yes 15% 11 mL/g 3.7 μmol/m2
Pursuit XRs Ultra Diphenyl 100 440 m2/g 1.5-8.0 Yes 14.6% 11 mL/g 2.6 μmol/m2
1. Doxepin
2. Imipramine
3. Nortriptyline
4. Amitriptyline
5. Trimipramine
Mobile Phase: 75% Methanol / 25% 50 mM
Pyrrolidine Buffer, pH 11.5
Flow Rate: 0.5 mL/min
Silica Base
Silica Base
Silica Base
α: 1.07 Rs: 1.15
α: 1.14 Rs: 2.42
m
Optimal selectivity for the beta blocker Pindolol.
ZORBAX Bonus-RP
•
•

•
• Polymer (USP L21)
• Pore size(100-4000)
.
R1
CH3
CH3
CH3
CH3
CH3
CH3
Si
SiO
O
0.0012
0.0014
0.0016
2. (Triple endcapping pH 7 )
3. SB-Aq
R
R
R
R
R
R
R1SiO
OH
OH
R1SiO
R1SiO
ZORBAX Bonus-RP 80 180 m2/g 60 °C 2.0-9.0 Triple 9.5%
Bonded Phase Pore Size
ZORBAX SB-Aq 80 180 m2/g 80 °C 1.0-8.0 No proprietary
Bonded Phase Pore Size
Surface Area
Carbon Load
End capped
Pore Volume
Liquid Coverage
Polaris C18-A 180 200 m2/g 13.8% Yes 1.1 cm3/g 3.9 μmol/m2
Polaris C8-A 180 200 m2/g 7.4% Yes 1.1 cm3/g 4.8 μmol/m2
Polaris C18-Ether 180 200 m2/g 12.1% Yes 1.1 cm3/g 3.3 μmol/m2
Polaris C8-Ether 180 200 m2/g 7.1% Yes 1.1 cm3/g 4.5 μmol/m2
Polaris Amide C18 180 200 m2/g 15% Yes 1.1 cm3/g 4.4 μmol/m2
Polaris NH2 180 200 m2/g 5.5% Amide 1.1 cm3/g 3.8 μmol/m2
Polaris Si-A 180 200 m2/g N/A N/A 1.1 cm3/g N/A
Column Specifications
Temperature Limits 200 °C
Maximum Pressure 5-8 μm: 3000 psi (210 bar) / 3 μm: 4000 psi (300 bar)
PLRP-S Applications
Columns : ZORBAX RRHT StableBond 4.6 x 50 mm 1.8 μm
Mobile Phase : A: pH 5 Na Acetate (USP method)
B: MeCN, (70:30)
Temperature : 25°C
Flow : 1.5 mL/min.
Detection : 219, 4 nm, 0.2 s response time, semi-micro flow cell
Sample : USP pindolol resolution solution
Column: ZORBAX Bonus-RP
Mobile Phase: 60% 25 mM
Phosphate Buffer,
Mobile Phase: 5% MeOH/95% water
(0.1% TFA)

HPLC UHPLC ! • 1.7μm Solid Core 0.5μm Porous Out-layer
• Sub-2μm 90% , 50%
• 3.5μm 2
• 600bar LC UHPLC
• 12
Solid Core
RRHT : Rapid Resolution High Throughput - 1.8μm / 600bar
RRHD : Rapid Resolution High Definition – 1.8μm / 1200bar
* RRHD .
4. 1.8μm 90% 50% Poroshell 120
5. ZORBAX RRHD Eclipse Plus C18 2.1 50mm, 1.8μm 7
Bonded Phase Pore Size Temp. Limits pH Range Endcapped Carbon Load
EC-C18 120 60 °C 2.0-8.0 Double 10%
EC-C8 120 60 °C 2.0-8.0 Double 5%
SB-C18 120 90 °C 1.0-8.0 No 8%
SB-C8 120 80 °C 1.0-8.0 No 5.5%
HPH-C18 100 60 °C 3.0-11.0 Double Proprietary
HPH-C8 100 60 °C 3.0-11.0 Double Proprietary
Phenyl-Hexyl 120 60 °C 2.0-8.0 Double 9%
SB-Aq 120 80 °C 1.0-8.0 No Proprietary
Bonus-RP 120 60 °C 2.0-9.0 Triple 9.5%
HILIC 120 60 °C 0.0-8.0 No N/A
EC-CN 120 60 °C 2.0-8.0 Double 3.5%
PFP 120 60 °C 2.0-8.0 Yes 5.1%
Bonded Phase Pore Size Surface area pH Range Endcapped
ZORBAX Eclipse Plus C18 95 160 m2/g 2.0-9.0 Yes
ZORBAX Eclipse Plus C8 95 160 m2/g 2.0-9.0 Yes
ZORBAX Eclipse XDB-C18 80 180 m2/g 2.0-9.0 Yes
ZORBAX Extend-C18 80 180 m2/g 2.0-11.5* Yes
ZORBAX StableBond SB-C18 80 180 m2/g 1.0-8.0* No
ZORBAX StableBond SB-C8 80 180 m2/g 1.0-8.0* No
ZORBAX StableBond SB-Phenyl 80 180 m2/g 1.0-8.0* No
ZORBAX StableBond SB-CN 80 180 m2/g 1.0-8.0* No
ZORBAX StableBond SB-Aq 80 180 m2/g 1.0-8.0* No
ZORBAX Eclipse PAH 95 160 m2/g 2.0-8.0 No
ZORBAX HILIC Plus 95 160 m2/g 0.0-8.0 No
2.5 5 7.5 10
0 .6
min
40% water
Ref = 360,100 nm
P/N 5188-6529 spiked
in water/acetonitrile (65:35)
Pressure 1017 bar
0 0 2
1
2
3
4
5
6
7
8
9
10
1.8 µm 2.7 µm
6. Benzoic Acid
7. Methyl Paraben
A: H2O (0.05 % TFA) B: ACN (0.04 % TFA) Time 0.0 95/5 A/B
Time 1.0 55/45 A/B
Time 1.1 0/100 A/B
Flow Rate: 1.7 mL/min
225 nm (0.46 min)
255 nm (0.67 min)
ZORBAX Normal-Phase Columns
• USP L10
• USP L8
Normal-Phase Columns
Bonded Phase Pore Size Surface Area pH Range Endcapped Carbon Load
ZORBAX Rx-SIL 80 180 m2/g 0-8.0 No
ZORBAX Eclipse XDB-CN 80 180 m2/g 2.0-8.0 Yes 4.3%
ZORBAX CN 70 300 m2/g 2.0-7.0 Yes 7%
ZORBAX NH2 70 300 m2/g 2.0-7.0 Yes 4%
ZORBAX HILIC Plus •
•
• LC/MS
Non-bonded silica 95 160 m2/g 0-8.0
Silica Base
Silica Base
Column : ZORBAX CN, 880952-705, 4.6 x 250 mm, 5 μm
Mobile Phase : A : Heptane
Hi-Plex
• ZORBAX SAX Column : Quaternary amine / ()
• ZORBAX SCX Column : Sulfonic acid , , / (+)
•
•
• Application
Conditions Sample : Organic acids Column : Agilent Hi-Plex H ( PL1170-6830) Mobile phase : 0.1 M H2SO4
Flow rate : 0.6 mL/min Temperature : 50 °C Detector : RI
1. Oxalic acid 2. cis-Aconitic acid 3. Tartaric acid 4. Malic acid 5. Lactic acid 6. Formic acid 7. Fumaric acid 8. Propionic acid 9. Butyric acid
1. Raffinose 2. Sucrose 3. Lactulose 4. Glucose 5. Galactose 6. Fructose 7. Ribitol 8. Mannitol 9. Sorbitol
Conditions Sample : Sugars and sugar alcohols Column : Agilent Hi-Plex Ca (Duo) ( PL1F70-6850) Sample size : 10 mg/mL Mobile phase : 100% DI H2O Flow rate : 0.4 mL/min Injection volume : 10 μL Temperature : 85 °C Detector : RI
6. Hi-Plex H 9
7. Hi-Plex Ca
Ion Exchange Columns
Bonded Phase Pore Size Surface Area pH Range Functionality Max Pressure
ZORBAX SAX 70 300 m2/g 2.0-7.0 Quaternary amine 350 bar
ZORBAX 300SCX 300 50 m2/g 2.0-7.0 Sulfonic acid 350 bar
Bonded Phase Temperature
Hi-Plex Ca 80-90 °C 0.6 Water
Hi-Plex Ca USP L19 80-90 °C 0.3 Water
Hi-Plex Pb 70-90 °C 0.6 Water
Hi-Plex H for carbohydrates 60-70 °C 0.6 Water
Hi-Plex H for organic acids 40-60 °C 0.6 Dilute Acid
Hi-Plex Ca (Duo) 80-90 °C 0.6 Water
Hi-Plex K 80-90 °C 0.6 Water
Hi-Plex Na (Octo) 80-90 °C 0.6 Water, Sodium Hydroxide
Hi-Plex Na 80-90 °C 0.3 Water
Application Area Recommended Column
USP Methods Specifying L17 Media Hi-Plex H
USP Methods Specifying L19 Media Hi-Plex Ca and Hi-Plex Ca (Duo)
USP Methods Specifying L34 Media Hi-Plex Pb
USP Methods Specifying L58 Media Hi-Plex Na and Hi-Plex Na (Octo)
Mono- and Disaccharides
Adulteration of Food and Beverages Hi-Plex Ca and Hi-Plex Pb
Food Additives Hi-Plex Ca and Hi-Plex Pb
Dairy Products Hi-Plex Ca and Hi-Plex H
Sweetened Dairy Products Hi-Plex Pb
Confectionery Hi-Plex Ca and Hi-Plex Pb
Fruit Juice Hi-Plex Ca
Oligosaccharides <Dp5 with Monosaccharides
Special Application Columns ZORBAX Carbohydrate Analysis
ZORBAX Amino Acid Analysis(AAA)
• Aminopropyl packing material
• 4.6 X150mm 50μL
• RID(Refractive index detectors)
Special Columns
Carbon Load
Time (min) 2 31 4 5 76
2 31 4 5 76
2 31 4 5 76
Xylose
Xylose
Xylose
Rhamnose
Rhamnose
Rhamnose
Xylitol
Xylitol
Lactose
Lactose
Fructose
Glucose
Mannitol
Glu/Gal
Sucrose
Sucrose
Maltose
9. 24
1
2
40
min0 2 4 6 8 10 12 14 16 18
0
mAU
min0 2 4 6 8 10 12 14 16 18
DAD1 B, Sig=262,16 Ref=324,8 (AMINO3\AA6-0003.D)
mAU DAD1 A, Sig=338,10 Ref=390,20 (AMINO3\AA6-0003.D)
40
min0 2 4 6 8 10 12 14 16 18
0
mAU
min0 2 4 6 8 10 12 14 16 18
DAD1 B, Sig=262,16 Ref=324,8 (AMINO3\AA6-0003.D)
• : 24
• Agilent
• Agilent Autosampler
Column Agilent ZORBAX Eclipse AAA 4.6×150 mm, 5 μm ( 993400-902)
Mobile Phase
A: 40 mM Sodium phosphate(Di-basic) 0.1% Phosphoric acid B: Acetonitrile / Methanol / DW = 45/45/10
Time (min) B (%)
0.0 0
1.9 0
18.1 57
18.6 80
22.3 80
23.2 0
26.0 0
Detector DAD 338nm (1 ), 262nm (2 ) FLD Ex : 340nm, Em : 450nm (1 ) Ex : 266nm, Em : 305nm (2 )
Flow rate 1.5 ml/min
Temp. 40

OPA reagent, 10 mg/mL each in 0.4 M borate butter o-phthalaldehyde (OPA) and 3-mercaptopropionic acid, 6 x 1 mL ampoules
5061-3335
5061-3337
• (1.8μm :<1200bar, 2.7μm Poroshell : <600bar)
• LC/MS LC/FLD
0
50
100
150
200
250
300
• Lot
• HPLC, UHPLC
• 600bar
• Peptide Mapping
Column: ZORBAX Carbohydrate Analysis, 843300-908, 4.6 x 150 mm, 5 μm Mobile Phase: 75% ACN/25% H2O Flow Rate: 2 mL/min Temperature: 30°C Detector: RID Det. Temp: 30°C Sample: (54 μg each), Inj. = 6.3 μL
Column: AdvanceBio Peptide Mapping, 2.1 x 250 mm, 2.7 μm, 651750-902 Flow Rate: 0.4 mL/min Injection: 5 μL (2.0 mg/mL) Temp: 55 °C Detection: 220 nm Gradient: A, water (0.1% FA); B, ACN (0.1% FA), 0-28 min, 3-45% B; 28-33 min, 45-60% B; 33-34 min, 60-95% B
SEC
Organic solvents Polar/mixed solvents Aqueous solvents (PS) MW range (PEG/PEO)
g/mol g/mol
0 0
PLgel 5 µm M
IXED-D 200 to 400,000
PLgel 5 µm M
IXED-C 200 to 2,000,000
6,000 to 10,000,000
4Å 10,000 to 450,000
PLgel 5/10 µm 10
5Å 60,000 to 1,700,000
200,000 to 10,000,000
The latest technology in GPC for increased speed and resolution
MW range Agilent PlusPore Columns High pore volume for very high resolution
Agilent PL Rapide Columns Special format for fast separations
Up to 10,000,000 PL Rapide H Up to 2,000,000 PolyPore PL Rapide M Up to 500,000 ResiPore PL Rapide L Up to 25,000 MesoPore Up to 3,300 OligoPore PL Rapide F
he laaaatetetetetetete tttstststststststst tttttttttececececececechhhhnhnhnhnhnhnhn lllololololololologogogogogogoggyyyyyyy y iiiininininininin GGGGGGGGGGPCPCPCPCPCPCPCPCPCPC ffffffffffororororororor iiiiiiiiiincncncncncncncrerererererereasasasasasasas dddededededededed ssssssspepepepepepepep dddededededededed aaaaaaa dddndndndndndndnd rrrrrrreseseseseseses lllolololololololol tutututututututiioioioion
GPC / SEC Columns & Standards
2,000~40,000,000
500~10,000,000
200~2,000,000
200~400,000
PLgel Olexis
PL HFIPgel HFIP
PLgel Preparative Prep PLgel
PL Rapide
PolarGel /
PlusPore
GPC/SEC
• GPC/SEC Calibration ISO 9001:2000
•
•
Polymer Type Individual MW
Polystyrene 3 3 3 3 Organic
Polymethylmethacrylate 3 3 3 Organic
Polyethylene glycol (PEG) 3 3 3 Organic/Aqueous
Polyethylene oxide (PEO) 3 3 3 Organic/Aqueous
Pullulan polysaccharide 3 3 Organic/Aqueous
Polyacrylic acid Na salt 3 3 Aqueous

Biosolution Columns
Agilent Bio-monolith Protein A
• Agilent Bio SEC-3 & Bio-5 : Pore size
• ProSEC 300S : Globular Protein
• ZORBAX GF-250 & GF-450 : Protein Preparative SEC
• PL aquagel-OH : Biopolymer
•
• PEEK Staninless Steel
• mAb
• Bio-monolith
Bonded Phase ID Particle Size pH
Stability Operating Temp Limit
0.1-1.0 mL/min
• Biomolecules
• 1,000kDa Protein
• pH (pH 2-11.5)
• pH Hydrophobic Peptides
• LC/MS Ammonium-hydroxide
• Pore size 300 Protein Peptide
•
•
Poroshell 300
Bonded Phase Pore Size Temp. Limits* pH Range Endcapped
Poroshell 300SB-C18, C8, C3 300 90 °C 1.0-8.0 No
Poroshell 300Extend-C18 300 40 °C above pH 8 60 °C below pH 8
2.0-11.0 Yes
Carbon Load
ZORBAX 300SB-C18 300 45 m2/g 90 °C 1.0-8.0 No 2.8%
ZORBAX 300SB-C8 300 45 m2/g 80 °C 1.0-8.0 No 1.5%
ZORBAX 300SB-C3 300 45 m2/g 80 °C 1.0-8.0 No 1.1%
ZORBAX 300SB-CN 300 45 m2/g 80 °C 1.0-8.0 No 1.2%
ZORBAX 300-Diphenyl 300 45 m2/g 80 °C 1.0-8.0 Yes 1.9%
Bonded Phase Pore Size
Carbon Load
ZORBAX 300 Extend-C18 300 45 m2/g 60 °C 2.0-11.5 Double 4%
Bio Columns
11
Application Agilent Columns
• RRHD 300SB-C18
• RRHD 300SB-C8
• RRHD 300SB-C3
• RRHD 300-Diphenyl
• RRHD 300-HILIC
ZORBAX 300 Extend-C18
Peptides and proteins up to 1,000 kDA, monoclonal antibodies and intact proteins
Poroshell 300
Poroshell 120
Application Agilent Columns
Large biomolecules and samples with multiple molecular weight components
Agilent Bio SEC-5
Low MW polymers and oligomers, oligosaccharides, PEGs, lignosulfonates
2 or 3 PL aquagel-OH
• PL aquagel-OH 8 μm
Very high MW polymers, hyaluronic acids, starches, gums
PL aquagel-OH 60/50/40 15 μm in series
Application Agilent Columns
Peptides and proteins Agilent Bio IEX
Proteins, peptides and deprotected
• 1000
• 4000
Antibodies (IgG, IgM), plasmid DNA,
viruses, phages and other macro
biomolecules
Bio-Monolith
Plasmid DNS, bacteriophages Bio-Monolith DEAE
Proteins, antibodies Bio-Monolith SO3
Ion-exchange Column
Affinity Column
Application .
Agilent LC USP code USP USP Packing Materials Column
L1 Octadecyl silane chemically bonded to porous silica or ceramic microparticles, 1.5 to 10 μm in diameter, or a monolithic rod
ZORBAX Eclipse Plus C18
Agilent Prep C18
L3 Porous silica particles, 1.5 to 10 μm in diameter, or a monolithic silica rod
ZORBAX HILIC Plus
Polaris Si-A
Agilent Prep
L7 Octylsilane chemically bonded to totally porous silica particles, 1.5 to 10 μm in diameter, or a monolithic silica rod
ZORBAX Eclipse Plus C8
L8 An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 3 to 10 μm in diameter
ZORBAX NH2
Polaris NH2
L9 Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic cation-exchange coating, 3 to 10 μm in diameter
ZORBAX SCX
L10 Nitrile groups chemically bonded to porous silica particles, 3 to 10 μm in diameter
ZORBAX CN
ZORBAX SB-CN
ZORBAX Eclipse XDB-CN
L11 Phenyl groups chemically bonded to porous silica particles, 1.5 to 10 μm in diameter
ZORBAX Eclipse XDB Phenyl
ZORBAX Eclipse Plus Phenyl-Hexyl
Pursuit DiPhenyl
L13 Trimethylsilane chemically bonded to porous silica particles, 3 to 10 μm in diameter
ZORBAX TMS
L14 Silica gel having a chemically bonded, strongly basic quaternary ammonium anion-exchange coating, 5 to 10 μm in diameter
ZORBAX SAX
IonoSpher A
USP USP Packing Materials Column
L15 Hexylsilane chemically bonded to totally porous silica particles, 3 to 10 μ m in diameter
MetaSil C6
Hi-Plex H
Hi-Plex Ca
Hi-Plex Ca (Duo)
L20 Dihydroxypropane groups chemically bonded to porous silica particles, 3 to 10 μm in diameter
LiChrospher Diol
L21 A rigid spherical styrenedivinylbenzene copolymer, 5 to 10 μm in diameter
PLgel
PLRP-S
L22 A cation-exchange resin made of porous polystyrene gel with sulfonic acid groups, about 10 μm in size
Hi-Plex H
L25 Packing having the capacity to separate compounds with a MW range from 1,000 to 5,000 da (as determined by the polyethylene oxide), applied to neutral, anionic and cationic water-soluble polymers. A polymethacrylate resin base, crosslinked with polyhydroxylated ether (surface contained some residual carboxyl functional gruops) was found suitable
PL aquagel-OH
L33 Packing having the capacity to separate dextrons by molecular size over a range of 4,000 to 500,000 da. It is spherical, silica-based, and processed to provide pH stability
ZORBAX GF-250
Bio SEC-3
Bio SEC-5
Hi-Plex Pb
ZORBAX GF – 250
ZORBAX GF – 450
L43 Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 5 to 10 μm in diameter
Pursuit PFP
Poroshell120 PFP
L45 Beta cyclodextrin bonded to porous silica particles, 5 to 10 μm in diameter
ChiraDex Chiral
L50 Multifunction resin with reversed-phase retention and strong anion- exhange functionalities. The resin consists of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 3 to 15 μm in diameter, and a surface area of not less than 350 m2 per g. Substrate is coated with quarternary ammonium functionalized latex particles consisting of styrene cross-linked with divinylbenzene
ZORBAX 300SCX
L52 Weak cation-exchange resin made of porous silica with sulfopropyl groups, 5 to 10 μm in diameter
IonoSpher C
L53 Weak cation-exchange resin consisting of ethylvinylbenzene, 55% crosslinked with divinylbenzene copolymer, 3 to 15 μm diameter. Substrate is surface grafted with carboxylic acid and/or phosphoric acid functionalized monomers. Capacity not less than 400 μEq/column
Bio SAX
L56 Propyl silane chemically bonded to totally porous silica particles, 3 to 10 μm in diameter
ZORBAX SB-C3
Ultron ES-OVM
L58 Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form, about 6 to 30 μm in diameter
Hi-Plex Na
Hi-Plex Na (Octo)
L60 Spherical, porous silica gel, 10 μm in diameter, the surface has been covalently modified with alkyl amide groups and endcapped
Polaris Amide-C18

ID FDA ± 50% USP ± 25%
± 50%
to 50%