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Download Infinity 2D-LC Solution Solution - Agilent The 1290 Infinity 2D-LC Solution • Based on the Agilent

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  • Page 1

    The Agilent 1290

    Infinity 2D-LC Solution

    Achieving highest separation power

    with the Agilent 1290 Infinity 2D-LC

    Solution

  • Page 2

    2D-LC - What is it?

    Peak capacities multiply for orthogonal separation mechanisms!

    Two different modes:

    Comprehensive 2D-LC (“LCxLC”)

    Heart-cutting 2D-LC (“LC-LC”)

    2DLC: Injecting the effluent or a part of the effluent of one column to a second

    column, ideally with orthogonal separation behavior.

    Purpose: increase total separation power.

    Complex/unknown samples:

    bio-pharma, food, polymers....

    Known samples/improving

    confidence: pharma, methdev...

  • Page 3

    2D-LC - What is it?

    Pump Autosampler Column Detector 1D-Chrom.

    Standard 1-dimensional LC:

  • Page 4

    2D-LC - What is it?

    Pump Autosampler Column 1

    Pump 2

    Column 2

    Injector

    Detector

    2-dimensional LC:

  • Page 5

    2D-LC - comprehensive vs. heart-cutting 2D-LC

    Comprehensive 2D-LC (LCxLC):

    LC1

    LC2

    1st peak from

    1st dimension

    2nd peak from

    1st dimension

    3rd peak from

    1st dimension

    LC1

    LC2

    min

    sec

  • Page 6

    Only parts of the effluent of the first column will be

    injected to the second column.

    Heart-cutting 2D-LC (LC-LC):

    2D-LC - comprehensive vs. heart-cutting 2D-LC

    LC1

    LC2

    The gradients in the 2nd dimension can be much

    longer as in comprehensive 2D-LC.

    Loss of information.

    But better quality data for peak of interest

  • Page 7

    The 1290 Infinity 2D-LC Solution

    • Based on the Agilent 1290 Infinity LC, thus highest performance for 2D-LC

    – Excellent retention time precision

    – High power range (up to 1200 bar, up to 5 ml/min)

    – Highest sensitivity

    • Highest flexibility on hardware set-up

    – Different pumps, autosamplers and detectors supported

    – Different detectors at different positions

    – Innovative new 2D-LC valve head , different valve set-up possibilities supported

    • Most easy-to-use 2D-LC acquisition SW

    – Unique features like automatically shifted gradients or peak-triggered operation

    – For comprehensive 2D-LC and heart-cutting 2D-LC

    • High performance data analysis by partner-solution

  • Page 8

    For heart-cutting 2D-LC by Agilent‘s OpenLAB CDS ChemStation ed.

    For comprehensive 2D-LC Agilent recommends:

    GC Image LCxLC Edition Software

    Key features offered:

    • Direct Agilent datafile import

    • Peak identification, integration, annotation

    • Comparative Analysis and Visualization

    • Compound-libraries

    • MS-data handling

    Data-Analysis

  • Page 9

    Hardware – Module-flexibility

    1. Dimension

    2. Dimension

    1290 Infinity

    Binary Pump

    1290 Infinity

    Binary Pump

    1290 Infinity

    Autosampler or 1260

    HiP Autosampler

    Optional

    1260/1290

    Infinity

    Detector

    One or two

    1290 Infinity

    TCC

    Optional

    1260/1290

    Infinity

    Detector

    1260/1290

    Infinity

    detector

    1260 Infinity Binary

    or Quaternary Pump

    1260 Infinity

    Capillary Pump 1260 Infinity

    Autosampler

    For 1st

    dimension

    chromatogram

    and peak-

    triggering

    To monitor

    waste-line

    Almost any Agilent pump or autosampler in the 1st dimension!

    Almost any detectors are supported!

    A 1290 Infinity Binary Pump for the 2nd dimension is required.

  • Page 10

    Hardware – Valves, uniqueness and flexibility

    1. Dimension

    2. Dimension

    waste

    Loop1

    Loop2

    2pos/4port-duo

    New Agilent-only special 2D-LC-QucikChange valve.

    Single valve with fully symmetric flow-paths and symmetric fill/flush-out

    behavior. Only valve that allows countercurrent flush-out of both loops.

  • Page 11

    Advantages of the new Agilent 2D-LC QuickChange valve head

    All flow paths are equal!

    Symmetric contercurrent or co-current fill/analyze direction of loops possible

    All in one valve

    Loop1

    Loop2

    waste

    2D-pump 2D-pump

    1D-column

    2D-column 2D-column

    Fill direction Analyze direction

    Valve

    switching

    1. 2.

  • Page 12

    Hardware – Valve-flexibility

    1. Dimension

    2. Dimension

    waste

    Loop1

    Loop2

    2pos/

    10port

    valve

    Most other valve configurations for comprehensive and heart-cutting

    2D-LC are supported as well. Easy transfer of existing 2D-LC methods!

  • Page 13

    Hardware – Valve-flexibility

    1. Dimension

    2. Dimension

    waste

    Loop1 Loop2

    2x

    2pos/6port

    valves

    Also dual valve-head configurations supported with automatic

    synchronisation of valve-drives!

  • Page 14

    Dashboard: All modules in one dashboard, all system

    parameters immediately visible

    2D-LC Acquisition Software

  • Page 15

    2D-LC Acquisition Software - supported 2D-LC modes

    comprehensive LCxLC

    Heart-cutting LC-LC

    Standard-repeating

    Time or peak-triggered

    Time-triggered

    Peak-triggered

  • Page 16

    Most easiest 2D-LC System Configuration - “One screen for the entire system”

  • Page 17

    Most easiest set-up of complex 2D-LC methods - 2D-LC specific parameters of the 2D-pump

  • Page 18

    Most easiest set-up of complex 2D-LC methods - Time-segmenting for the 2nd dimension

  • Page 19

    Time-segmenting in comprehensive 2D-LC - Reducing cost!

    1D Gradient/

    2D Gradients

    Idle flow rate

    2D Pump flow

    rate

    2D Pump flow rate

    Solvent saving!!!

    2D Time-segments

    set in software idle

    idle

    idle

    Time-triggered

    Peak-triggered

    Valve toggling

    Increase life time!

    %B (2D)

    %B (1D)

    F (2D)

    1D Chromatogram

  • Page 20

    Time-segmenting in comprehensive 2D-LC - Reducing cost!

    1D Gradient/

    2D Gradients

    Idle flow rate

    2D Pump flow

    rate

    2D Pump flow rate

    Solvent saving!!!

    2D Time-segments

    set in software idle

    idle

    idle

    Time-triggered

    Peak-triggered

    Valve toggling

    Increase life time!

    %B (2D)

    %B (1D)

    F (2D)

    1D Chromatogram

  • Page 21

    Time-segmenting in comprehensive 2D-LC - Reducing cost!

    1D Gradient/

    2D Gradients

    Idle flow rate

    2D Pump flow

    rate

    2D Pump flow rate

    Solvent saving!!!

    2D Time-segments

    set in software idle

    idle

    idle

    Time-triggered

    Peak-triggered

    Valve toggling

    Increase life time!

    %B (2D)

    %B (1D)

    F (2D)

    1D Chromatogram

  • Page 22

    Time-segmenting in comprehensive 2D-LC - Reducing cost!

    1D Gradient/

    2D Gradients

    Idle flow rate

    2D Pump flow

    rate

    2D Pump flow rate

    Solvent saving!!!

    2D Time-segments

    set in software idle

    idle

    idle

    Time-triggered

    Peak-triggered

    Valve toggling

    Increase life time!

    %B (2D)

    %B (1D)

    F (2D)

    1D Chromatogram

  • Page 23

    Example: graphical editing of a gradient shift - replace editing of large timetables by a few mouse operations -

    1 Use context menu to enter the editing mode

    3 Draw a straight line by dragging the mouse to a new %B value at a

    specified runtime of the 1st dimension

    2 Timetable entries are marked with circles

    4 When releasing the mouse, a new TT entry is made and the gradient

    rollout is automatically updated

    6 Insert / Delete shift points (mouse cursor and context menu changes

    near to a shift line)

    5 Repeat step 3 + 4 with other TT entries

  • Page 24

    2D-LC Acquisition Sofware - supported 2D-gradients modes

    Isocratic Advancing Isocratic

    Std. Gradient Shifted Gradient

    …and most combinations!

  • Page 25

    2D-LC Acquisition Sofware - supported 2D-gradients modes

    Isocratic Advancing Isocratic

    Std. Gradient Shifted Gradient

    • Start and end time

    • Peak-triggered

    • Start and end time

    • Peak-triggered

    • Stepwise or grad

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