you’ve come a long way, dolly! · 2020-08-11 · • classroom set of mock newspaper articles...

14
Teacher Guide © 2020 University of Utah Updated July 27, 2020 1 You’ve Come A Long Way, Dolly! Abstract During this activity, students construct a timeline depicting the history of cloning. They present, and then place in order, key events in cloning using the birth of Dolly the sheep as a reference point. Since students are not given the date of each event, they need to consider the relative progression of cloning techniques and the increasing complexity of cloned organisms. Once the timeline is complete and the dates are confirmed, students are asked to consider and discuss the scientific and social/political significance of the events. Learning Objectives Students will understand that cloning is not a new science Students will understand that scientific advances are a process with one “discovery” enabling the next Students will think critically about the social or political impact of scientific progress Students will learn about the advance of cloning technologies and the current state of cloning science Estimated time Class Time 70 minutes (may be extended by class discussion or assessment) Prep time 30 minutes Materials Classroom set of Mock Newspaper Articles (cut each page in half along dotted line) Student handouts Tape Stickers in two colors for each student Background Current media attention seems to indicate that cloning is a recent scientific phenomenon. However, less-publicized studies in cloning have been carried out since 1885. The steps leading to current cloning methods began with cloning very simple organisms by artificial embryo twinning (splitting a very early embryo in half in a Petri dish). Some of the first cloned organ- isms came from embryos that were split in two using a fine piece of hair as a noose. Further experi- ments using this technique established the fact that the nucleus directs cell growth and division. The next step was cloning by nuclear transfer. This is the process of taking the nucleus from a donor cell and placing it in an unfertilized, enucleated egg cell. Experiments using this technique began with simple organisms and progressed to more complex mammals. Dolly the sheep was the first organism to be cloned using the nucleus from an adult somatic cell as opposed to an embryonic cell, thus indicating yet another advance in cloning technology.

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Page 1: You’ve Come A Long Way, Dolly! · 2020-08-11 · • Classroom set of Mock Newspaper Articles (cut each page in half along dotted line) • Student handouts • Tape • Stickers

Teacher Guide

© 2020 University of Utah Updated July 27, 2020 1

You’ve Come A Long Way, Dolly!AbstractDuring this activity, students construct a timeline depicting the history of cloning. They present, and then place in order, key events in cloning using the birth of Dolly the sheep as a reference point. Since students are not given the date of each event, they need to consider the relative progression of cloning techniques and the increasing complexity of cloned organisms. Once the timeline is complete and the dates are confirmed, students are asked to consider and discuss the scientific and social/political significance of the events.

Learning Objectives• Students will understand that cloning is not a new science

• Students will understand that scientific advances are a process with one “discovery” enabling the next

• Students will think critically about the social or political impact of scientific progress

• Students will learn about the advance of cloning technologies and the current state of cloning science

Estimated time• Class Time 70 minutes (may be extended by class discussion or assessment)

• Prep time 30 minutes

Materials• Classroom set of Mock Newspaper Articles (cut each page in half along dotted line)

• Student handouts

• Tape

• Stickers in two colors for each student

BackgroundCurrent media attention seems to indicate that cloning is a recent scientific phenomenon. However, less-publicized studies in cloning have been carried out since 1885.

The steps leading to current cloning methods began with cloning very simple organisms by artificial embryo twinning (splitting a very early embryo in half in a Petri dish). Some of the first cloned organ-isms came from embryos that were split in two using a fine piece of hair as a noose. Further experi-ments using this technique established the fact that the nucleus directs cell growth and division.

The next step was cloning by nuclear transfer. This is the process of taking the nucleus from a donor cell and placing it in an unfertilized, enucleated egg cell. Experiments using this technique began with simple organisms and progressed to more complex mammals. Dolly the sheep was the first organism to be cloned using the nucleus from an adult somatic cell as opposed to an embryonic cell, thus indicating yet another advance in cloning technology.

Page 2: You’ve Come A Long Way, Dolly! · 2020-08-11 · • Classroom set of Mock Newspaper Articles (cut each page in half along dotted line) • Student handouts • Tape • Stickers

© 2020 University of Utah You’ve Come A Long Way, Dolly! — Teacher Guide 2

Cloning technology has also been used to create transgenic organisms by placing selected genes in the nuclei of cultured adult somatic cells and then using those nuclei to create transgenic cloned embryos. When grown to maturity, these organisms can be used to produce harvestable proteins for human use. Scientists are working on using cloning techniques to harvest human stem cells for medical treatment as well.

It is important that students understand the difference between the embryo twinning and somatic cell nuclear transfer cloning techniques. Since the embryo used for embryo twinning was produced by sexual reproduction, none of the resulting clones will be genetically identical to either parent. This is also true for cloning via nuclear transfer using nuclei from embryonic donor cells. However, since the nucleus used for somatic cell nuclear transfer is taken from one of a donor’s somatic cells, the clone is genetically identical to this “parent.” This ability to use an adult cell eliminates the need for an embryo, enabling clones to be produced by asexual rather than sexual reproduction.

Activity Part One:1. Begin class by discussing the significance of the birth of Dolly the sheep and the fact that Dolly’s

birth is just one event in the long history of cloning.

2. Tell the students that:

• They will be using The Cloning Times newspaper articles to construct a timeline depicting the history of cloning. Each article describes a significant event in the timeline. The name of the scientist who carried out the research or a person important to that event is listed after the headlines. A sentence summarizing the event is printed in bold type at the beginning of each article.

• As there are no dates on the articles, it is up to them to determine where to place their event along the timeline (a designated space along the wall of the classroom). Initially, they will use the birth of Dolly as a reference point.

• To construct the timeline, they will present their article headline and summary sentence to the class, and then tape the article on the wall in the order they think it belongs relative to events already posted. They will probably make many adjustments along the way.

3. Hand out articles and student handouts. Ask the students to read the Activity Instructions and begin.

4. Next, ask the student/group whose headline reads: Hello Dolly! to read their headline and sum-mary sentence (in boldface type) aloud to the class. Have that student tape their article in the middle of the space you have designated for timeline construction. This student must go first as their event is the reference point.

5. Call on students at random (or ask for volunteers) to read the headline and summary sentence (in boldface type) of their newspaper article aloud. After each presentation, have the student place the article along the timeline in the area they think it belongs, and ask them to explain why they are placing their event in that particular place. Students who are unsure of where to place their article may ask for input from the rest of the class.

6. When student presenters read the following three events, stop, and ask the class to write that summary sentence in the proper space on the Cloning Times Record. You will need to tell them the year of the event.

Page 3: You’ve Come A Long Way, Dolly! · 2020-08-11 · • Classroom set of Mock Newspaper Articles (cut each page in half along dotted line) • Student handouts • Tape • Stickers

© 2020 University of Utah You’ve Come A Long Way, Dolly! — Teacher Guide 3

• Frogs cloned by nuclear transfer from a tadpole embryo (1952)

• Female mouse cloned by somatic cell nuclear transfer (1998)

• Human cloning might also be used to create stem cells for new medical treatments (2001)

• This will help keep the students focused during the activity and provide additional refer-ence points along the way. Use these events to rearrange the posted newspaper events as necessary.

7. When students have placed their newspaper articles in the proper order, the large, light letters in the background will spell out “You’ve Come A Long Way Dolly”. Once all of the events are cor-rectly placed, give students time to copy the headlines and summary sentences down in order on their Cloning Times Record, thus revealing the actual date of each event.

Activity Part Two:Designate one sticker color to represent scientific significance and the other sticker color to repre-sent political/social significance. Give the students one sticker of each color.

8. Ask students to first place the designated sticker on the timeline event they think is of the most scientific importance. Discuss these choices as a class.

9. Next, ask students to place the other sticker on the event they think has the most social or politi-cal importance. Discuss these choices as a class.

Activity Part Three:10. Use the Questions (Part III) on the Cloning Timeline Activity Discussion and Questions student

handout (S-3) in one of the following ways:

• Discuss the questions as a class.

• Ask students to choose a question and write a one-page response using information from the constructed timeline to support their answer.

• Have students chose one question to answer by drawing a mural, comic strip, or flip-book using information from the constructed timeline.

• Assign each question to a small group to discuss and present their answer to the rest of the class.

Adaptations• This activity can be done as a whole class or within smaller groups.

• Ask students to discuss their articles in small groups and decide on an order before beginning their presentations.

• Have students use a highlighter to highlight key elements (in addition to the headline) in their newspaper article before presenting.

• Ask students in more advanced classes to present an article summary, in addition to the title and summary sentence, to the class

Funding for this module was provided by a Science Education Partnership Award (No. 1 R25 RR16291-01) from the National Center for Research Resources, a component of the National Institutes of Health.

Page 4: You’ve Come A Long Way, Dolly! · 2020-08-11 · • Classroom set of Mock Newspaper Articles (cut each page in half along dotted line) • Student handouts • Tape • Stickers

OY

Tech

niqu

e

Seei

ng D

oubl

e? S

ea U

rchi

ns C

lone

d!H

ans

Ado

lf E

dwar

d D

reis

ch

Sea

urch

ins

clon

ed

by

shak

ing

one

embr

yo in

to

two

sepa

rate

cel

ls.

Can

the

earli

est e

mbr

yos b

e sp

lit

into

indi

vidu

al c

ells,

whi

ch g

o on

to

bec

ome

sepa

rate

org

anism

s?

Doe

s ea

ch

embr

yoni

c ce

ll co

ntai

n a

com

plet

e se

t of

ge

netic

mat

eria

l tha

t ca

n di

rect

th

e fo

rmat

ion

of a

n or

gani

sm?

The

sea

urch

in i

s a

rela

tivel

y sim

ple

orga

nism

that

is u

sefu

l for

st

udyi

ng d

evel

opm

ent.

Dre

isch

show

ed t

hat

by m

erel

y sh

akin

g tw

o-ce

lled

sea

urch

in e

mbr

yos,

it w

as p

ossib

le t

o se

para

te t

he

cells

. Onc

e se

para

ted,

eac

h ce

ll gr

ew in

to a

com

plet

e se

a ur

chin

.

Wha

t di

d th

is te

ll us

? Ea

ch

cell

in

the

embr

yo

has

its

own

com

plet

e se

t of

gen

etic

in

stru

ctio

ns a

nd c

an g

row

int

o a

full

orga

nism

. Thi

s w

as th

e fir

st

ever

dem

onst

ratio

n of

clo

ning

by

em

bryo

twin

ning

.

Slim

y Sa

lam

ande

rs C

lone

d: W

hat’s

Nex

t?H

ans

Spem

ann

Sala

man

der

clon

ed

by

usin

g a

noos

e to

sepa

rate

th

e ce

lls

in

an

early

em

bryo

.A

sea

urch

in h

as s

ucce

ssfu

lly

been

cl

oned

us

ing

embr

yo

twin

ning

. Now

, can

this

be d

one

in a

mor

e co

mpl

ex o

rgan

ism

– sa

y, so

met

hing

with

a r

eal

back

bone

, lik

e a

sala

man

der?

W

ill t

he t

win

ning

app

roac

h st

ill

wor

k?

Spem

ann’

s fir

st c

halle

nge

was

to

figu

re o

ut h

ow to

split

the

two

cells

of a

n em

bryo

muc

h st

icki

er

than

sea

urc

hin

cells

. Cou

ld h

e tie

off

the

two

cells

with

a le

ngth

of

thre

ad, d

enta

l flos

s, or

eve

n a

stra

nd o

f hai

r?

Yes!

Spe

man

n fa

shio

ned

a tin

y no

ose

from

a st

rand

of b

aby

hair

and

tight

ened

it

betw

een

two

cells

of

a sa

lam

ande

r em

bryo

un

til t

hey

sepa

rate

d. E

ach

cell

grew

into

an

adul

t sa

lam

ande

r. Sp

eman

n al

so t

ried

to d

ivid

e m

ore

adva

nced

sa

lam

ande

r em

bryo

s us

ing

this

met

hod,

but

he

fou

nd t

hat

cells

fro

m t

hese

em

bryo

s w

eren

’t as

suc

cess

ful

at

deve

lopi

ng

into

ad

ult

sala

man

ders

.W

hat

did

this

tell

us?

Embr

yos

from

mor

e co

mpl

ex o

rgan

isms

can

also

be

“tw

inne

d” t

o fo

rm

mul

tiple

or

gani

sms

– bu

t on

ly u

p to

a c

erta

in s

tage

in

deve

lopm

ent.

Tech

niqu

e

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Shak

e to

sep

arat

e ce

ll

Two

iden

tica

l sea

ur

chin

sTw

o id

enti

cal

sala

man

ders

Two-

celle

d se

a ur

chin

em

bryo

Bab

y ha

ir n

oose

sep

arat

es s

tick

y ce

lls o

f tw

o-ce

lled

embr

yo

Page 5: You’ve Come A Long Way, Dolly! · 2020-08-11 · • Classroom set of Mock Newspaper Articles (cut each page in half along dotted line) • Student handouts • Tape • Stickers

’VU

Tech

niqu

eIt’s

True

: The

Nuc

leus

is in

Cha

rge

Han

s Sp

eman

n

The

cell

nucl

eus

cont

rols

th

e de

velo

pmen

t of a

sal-

aman

der e

mbr

yo.

Wha

t par

t of t

he e

mbr

yoni

c sa

l-am

ande

r ce

ll di

rect

s its

gro

wth

an

d di

visio

n? I

s it t

he n

ucle

us?

Usin

g a

high

-tech

gizm

o –

a st

rand

of

baby

hai

r tie

d in

to a

no

ose

– Sp

eman

n te

mpo

raril

y sq

ueez

ed a

sal

aman

der’s

fer

til-

ized

egg

to p

ush

the

nucl

eus

to

one

side

of th

e cy

topl

asm

. Th

e eg

g di

vide

d in

to m

ore

embr

y-on

ic c

ells

only

on

the

side

of th

e no

ose

with

the

nucl

eus.

Afte

r a

few

cel

l di

visio

ns,

Spe-

man

n lo

osen

ed t

he n

oose

, le

t-tin

g th

e nu

cleu

s sli

de b

ack

to it

s or

igin

al p

ositi

on in

the

cell.

Spem

ann

then

use

d hi

s no

ose

to s

epar

ate

this

“new

” ce

ll fro

m

the

rest

of t

he e

mbr

yo. S

ince

the

nucl

eus

was

now

in th

e or

igin

al

cyto

plas

m,

this

cell

rese

mbl

ed

a fe

rtili

zed

egg

mor

e th

an a

n em

bryo

nic

cell.

N

ever

thel

ess,

this

singl

e ce

ll gr

ew in

to a

new

sa

lam

ande

r em

bryo

.

Wha

t did

this

tell

us?

The

nucl

e-us

from

an

early

em

bryo

nic

cell

dire

cts

the

com

plet

e gr

owth

of

a s

alam

ande

r. E

ssen

-tia

lly t

he fi

rst

inst

ance

of

nucl

ear

trans

fer,

this

ex-

perim

ent s

how

ed th

at a

n em

bryo

nic

cell

nucl

eus

coul

d su

bstit

ute

for

the

nucl

eus i

n a

fert

ilize

d eg

g ce

ll.

Frog

s G

alor

e! N

ucle

ar T

rans

fer B

ecom

es a

Rea

lity

Rob

ert

Bri

ggs

and

Thom

as K

ing

Frog

clo

ned

by n

ucle

ar

tran

sfer

fro

m a

tad

pole

em

bryo

.Fa

scin

ated

by

the

idea

of

nucl

ear

tran

sfer

, Br

iggs

and

Kin

g w

ante

d to

see

whe

ther

the

y co

uld

use

this

tech

niqu

e to

clo

ne a

n ev

en m

ore

com

plex

org

anism

: the

frog

.

Thei

r fir

st c

halle

nge

was

to

isol

ate

the

nucl

eus

from

a d

onor

cel

l: in

this

case

, a

cell

from

an

early

tad

pole

em

bryo

. Nex

t, th

ey h

ad to

pre

pare

a

reci

pien

t fro

g eg

g ce

ll by

rem

ovin

g its

nuc

leus

– a

proc

ess c

alle

d en

ucle

-at

ion.

Las

t, th

e do

nor

nucl

eus

and

the

reci

pien

t eg

g ce

ll w

ere

unite

d.

Even

if

all

thes

e pr

oced

ures

wer

e su

cces

sful

, wou

ld th

e ne

w

“fer

tilize

d

egg”

de

velo

p in

to

a ta

dpol

e?

The

scie

ntis

ts c

reat

ed m

any

norm

al

tadp

ole

clon

es u

sing

nucl

ei fr

om e

ar-

ly e

mbr

yos.

But

just

like

Spe

man

n’s

sala

man

der

expe

rimen

ts,

clon

ing

was

less

succ

essf

ul w

ith d

onor

nuc

lei

from

mor

e ad

vanc

ed e

mbr

yos:

the

fe

w t

adpo

le c

lone

s th

at d

id s

urvi

ve

grew

abn

orm

ally

.

Wha

t di

d th

is te

ll us

? M

ost

impo

r-ta

ntly,

thi

s ex

perim

ent

show

ed t

hat

nucl

ear t

rans

fer w

as a

via

ble

clon

ing

tech

niqu

e. It

als

o re

info

rced

two

ear-

lier

obse

rvat

ions

. Firs

t, th

e nu

cleu

s di

rect

s ce

ll gr

owth

and

, ul

timat

ely,

an

orga

nism

’s de

velo

pmen

t. Se

c-on

d, e

mbr

yoni

c ce

lls e

arly

in d

evel

-op

men

t ar

e be

tter

for

clon

ing

than

ce

lls a

t lat

er s

tage

s.Te

chni

que

Don

or c

ell s

epar

ated

fr

om t

adpo

le e

mbr

yo

Cell

grow

s in

to s

alam

ande

r

Noo

se p

ushe

s nu

cleu

s to

on

e si

de o

f egg

cel

l

Noo

se lo

osen

ed; r

etur

ning

nu

cleu

s to

ori

gina

l pos

itio

n

Nuc

leus

rem

oved

from

egg

cel

l

Nuc

leus

rem

oved

fr

om e

mbr

yo c

ell

Embr

yo c

ell n

ucle

us

inse

rted

into

egg

cel

l

Cell

grow

s in

to

tadp

ole

clon

e

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Cell

sepa

rate

d fr

om e

mbr

yo

Cell

divi

sion

Page 6: You’ve Come A Long Way, Dolly! · 2020-08-11 · • Classroom set of Mock Newspaper Articles (cut each page in half along dotted line) • Student handouts • Tape • Stickers

CE

Tech

niqu

e

For C

loni

ng, A

ny N

ucle

us W

ill D

oJo

hn G

urdo

n

Frog

clo

ned

by n

ucle

ar

tran

sfer

fro

m a

diff

eren

-tia

ted

tadp

ole

cell.

Can

the

nucl

eus

from

an

adul

t ce

ll, m

ore

diffe

rent

iate

d th

an a

n em

bryo

nic c

ell, s

erve

as a

dono

r?

To fi

nd o

ut, G

urdo

n tra

nspl

ante

d th

e nu

cleu

s of a

tadp

ole

stom

ach

cell

into

an

enuc

leat

ed fr

og e

gg.

In th

is w

ay, h

e cr

eate

d ta

dpol

es

that

wer

e ge

netic

ally

iden

tical

to

the

one

from

whi

ch th

e st

omac

h ce

ll w

as ta

ken.

Wha

t di

d th

is te

ll us

? N

ucle

i fro

m fu

lly d

iffer

entia

ted

som

atic

ce

lls c

an b

e us

ed f

or c

loni

ng.

This

mea

ns t

hat

that

the

re i

s no

los

s of

gen

etic

mat

eria

l as

ce

lls in

an

orga

nism

div

ide

and

diffe

rent

iate

.

Clon

ing

wit

h a

Wee

Wit

tle

Wab

bit E

ggJ.

Der

ek B

rom

hall

Rabb

it cl

oned

by

em

bryo

nic

cell

nucl

ear

trans

fer.

The

next

clo

ning

cha

lleng

e w

as

to tr

y nu

clea

r tra

nsfe

r in

a m

ore

com

plex

mam

mal

ian

orga

nism

, lik

e th

e ra

bbit.

Mam

mal

ian

egg

cells

are

muc

h sm

alle

r tha

n th

ose

of fr

ogs

or s

alam

ande

rs, s

o th

ey

are

hard

er to

man

ipul

ate.

U

sing

glas

s pi

pette

s as

tin

y st

raw

s, Br

omha

ll tra

nsfe

rred

the

nucl

eus

from

a r

abbi

t em

bryo

ce

ll in

to a

n en

ucle

ated

rab

bit

egg

cell.

H

e co

nsid

ered

th

e pr

oced

ure

a su

cces

s w

hen

a m

orul

a, o

r ad

vanc

ed e

mbr

yo,

deve

lope

d af

ter

a co

uple

of

da

ys.

Wha

t di

d th

is te

ll us

? C

loni

ng

mam

mal

s us

ing

nucl

ear t

rans

fer

is po

ssib

le.

Tech

niqu

eD

onor

cel

l ext

ract

ed

from

rab

bit

embr

yo

Nuc

leus

rem

oved

from

egg

cel

l

Nuc

leus

rem

oved

fr

om e

mbr

yo c

ell

Embr

yo c

ell n

ucle

us

inse

rted

into

egg

cel

l

Cell

grow

s in

to

rabb

it m

orul

a

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Don

or c

ell e

xtra

cted

fr

om t

adpo

le s

tom

ach

Nuc

leus

rem

oved

from

egg

cel

l

Nuc

leus

rem

oved

fr

om s

tom

ach

cell

Stom

ach

cell

nucl

eus

inse

rted

into

egg

cel

l

Cell

grow

s in

to

tadp

ole

clon

e

Page 7: You’ve Come A Long Way, Dolly! · 2020-08-11 · • Classroom set of Mock Newspaper Articles (cut each page in half along dotted line) • Student handouts • Tape • Stickers

MO

Tech

niqu

e

Baa-

Baa

from

an

Eart

ly K

ind

of C

ell

Stee

n W

illa

rdse

n

Shee

p cl

oned

by

embr

y-on

ic c

ell n

ucle

ar tr

ansf

er.

Wou

ld n

ucle

ar t

rans

fer

wor

k in

sh

eep?

To

find

out,

Will

adse

n ch

emic

ally

sep

arat

ed o

ne c

ell

from

an

early

-sta

ge l

amb

em-

bryo

and

fuse

d it

to a

n en

ucle

at-

ed e

gg ce

ll. H

e th

en u

sed

a sm

all

elec

tric

shoc

k to

mim

ic fe

rtili

za-

tion

by a

sper

m c

ell,

caus

ing

the

new

cel

l to

divi

de.

Fina

lly,

Will

adse

n pl

aced

th

e re

sulti

ng e

mbr

yo in

to th

e w

omb

of a

sur

roga

te m

othe

r sh

eep

to

nurt

ure

it th

roug

h pr

egna

ncy.

This

was

the

first

larg

e m

amm

al

to b

e cl

oned

by

nucl

ear t

rans

fer

from

an

embr

yoni

c ce

ll.

Wha

t di

d th

is te

ll us

? C

loni

ng

by n

ucle

ar tr

ansf

er is

pos

sible

in

larg

er m

amm

als.

Moo

ove

Ove

r for

Hav

ing

a Co

w!

Nea

l Fir

st, R

anda

l Pra

ther

, and

Wil

lard

Eye

ston

e

Cow

cl

oned

by

em

bryo

nic

cell

nucl

ear

trans

fer.

Rabb

its,

shee

p –

can

even

la

rger

ani

mal

s, su

ch a

s ca

ttle,

be

clo

ned?

Firs

t, Pr

athe

r an

d Ey

esto

ne u

sed

a sm

all e

lect

rical

sh

ock

to f

use

early

-sta

ge c

ow

embr

yoni

c ce

lls t

o en

ucle

ated

eg

g ce

lls.

They

im

plan

ted

the

resu

lting

em

bryo

s in

to

the

wom

bs

of

surro

gate

m

othe

r co

ws

to n

urtu

re t

hem

thr

ough

pr

egna

ncy.

The

first

two

clon

ed

calv

es w

ere

nam

ed F

usio

n an

d C

opy.

Wha

t di

d th

is te

ll us

? Th

is ex

perim

ent

leng

then

ed

the

list

of m

amm

als

that

cou

ld b

e cl

oned

by

nucl

ear

trans

fer.

Still

, m

amm

alia

n cl

onin

g w

as li

mite

d to

us

ing

embr

yoni

c ce

lls

as

nucl

ei

dono

rs.

Clo

ning

us

ing

nucl

ei f

rom

diff

eren

tiate

d ad

ult

som

atic

ce

lls

was

n’t

thou

ght

poss

ible

.w

Tech

niqu

eD

onor

cel

l ext

ract

ed

from

cow

em

bryo

Nuc

leus

rem

oved

from

egg

cel

l

Embr

yo c

ell n

ucle

us

mov

es t

o eg

g ce

ll

Elec

tric

sho

ck f

uses

em

bryo

cel

l wit

h eg

g ce

ll

Cell

grow

s in

to

cow

clo

ne

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Don

or c

ell e

xtra

cted

fr

om s

heep

em

bryo

Nuc

leus

rem

oved

from

egg

cel

l

Nuc

leus

rem

oved

fr

om e

mbr

yo c

ell Em

bryo

cel

l nuc

leus

in

sert

ed in

to e

gg c

ell

Cell

grow

s in

to

shee

p cl

one

Elec

tric

sho

ck

Page 8: You’ve Come A Long Way, Dolly! · 2020-08-11 · • Classroom set of Mock Newspaper Articles (cut each page in half along dotted line) • Student handouts • Tape • Stickers

AE

Clon

ing

Law

s A

ppea

r on

the

Hor

izon

Wil

liam

Jeff

erso

n C

lint

on

U.S.

Pr

esid

ent

Clin

ton

requ

este

d le

gisl

atio

n to

pr

otec

t hu

man

res

earc

h su

bjec

ts.

As c

loni

ng te

chni

ques

impr

oved

hu

man

clo

ning

see

med

mor

e po

ssib

le,

and

the

issue

beg

an

to

appe

ar

on

polic

ymak

ers’

agen

das.

In

1995

, Pr

esid

ent

Clin

ton

form

ed

the

Nat

iona

l Bi

oeth

ics

Advi

sory

Co

unci

l (N

BAC

). Th

is co

unci

l, m

ade

up

of s

cien

tific

expe

rts a

nd n

on-

scie

ntist

s, ev

alua

ted

ethi

cal,

relig

ious

an

d le

gal

issue

s co

ncer

ning

th

e pr

otec

tion

of

hum

an r

esea

rch

subj

ects

. Th

is w

ould

lat

er b

e re

leva

nt t

o th

e co

ntro

vers

ies

surro

undi

ng

hum

an c

loni

ng.

Wha

t di

d th

is te

ll us

? Ju

st

beca

use

som

ethi

ng is

tech

nica

lly

poss

ible

doe

s no

t m

ean

that

it

is so

cial

ly r

espo

nsib

le.

In a

de

moc

ratic

soc

iety

, man

y po

ints

of

vie

w a

re c

onsid

ered

bef

ore

law

s are

pas

sed.

Baa-

Baa

Tim

es T

wo

from

a P

etri

Dis

h Ce

ll Zo

oIa

n W

ilm

ut a

nd K

eith

Cam

pbel

l

Shee

p cl

oned

by

nucl

ear

trans

fer f

rom

cel

ls g

row

n in

the

labo

rato

ry.

All

prev

ious

cl

onin

g ex

perim

ents

use

d do

nor

nucl

ei

from

ce

lls

that

w

ere

part

of

deve

lopi

ng

embr

yos.

In

thei

r ne

xt

expe

rimen

t, W

ilmut

an

d Ca

mpb

ell

used

do

nor

nucl

ei

from

a s

light

ly d

iffer

ent

sour

ce:

cultu

red

mam

mal

ian

cells

, whi

ch

wer

e ke

pt a

live

in th

e la

bora

tory

.W

ilmut

an

d Ca

mpb

ell

trans

ferre

d th

e nu

clei

fro

m

cultu

red

cells

int

o en

ucle

ated

sh

eep

egg

cells

. The

lam

bs b

orn

from

this

proc

edur

e w

ere

nam

ed

Meg

an a

nd M

olly.

Wha

t di

d th

is te

ll us

? Ce

lls

cultu

red

in t

he l

abor

ator

y ca

n al

so

supp

ly

dono

r nu

clei

fo

r cl

onin

g by

nuc

lear

tran

sfer

.

Tech

niqu

eD

onor

she

ep c

ell f

rom

la

bora

tory

cel

l cul

ture

Nuc

leus

rem

oved

from

egg

cel

l

Cul

ture

d ce

ll nu

cleu

s m

oves

into

egg

cel

l

Elec

tric

sho

ck f

uses

cu

ltur

ed c

ell w

ith

egg

cell

Cell

grow

s in

to

shee

p cl

one

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Page 9: You’ve Come A Long Way, Dolly! · 2020-08-11 · • Classroom set of Mock Newspaper Articles (cut each page in half along dotted line) • Student handouts • Tape • Stickers

OL

Tech

niqu

e

Hel

lo D

olly

!Ia

n W

ilm

ut a

nd K

eith

Cam

pbel

l

Shee

p cl

oned

by

som

atic

ce

ll nu

clea

r tra

nsfe

r.

One

of t

he b

igge

st c

halle

nges

in

mam

mal

ian

clon

ing

was

usin

g a

diffe

rent

iate

d ad

ult

som

atic

cel

l as

the

don

or. W

hat

was

the

big

de

al?

Ever

y ce

ll’s

nucl

eus

cont

ains

a

com

plet

e se

t of

ge

netic

in

form

atio

n.

How

ever

, th

e pa

ttern

of u

sing

this

info

rmat

ion

diffe

rs b

etw

een

adul

t ce

lls a

nd

embr

yoni

c ce

lls. T

his

mea

ns th

at

whe

n an

adul

t cel

l nuc

leus

is u

sed

as a

dono

r, its

gen

etic

info

rmat

ion

mus

t be

rese

t, or

re-b

oote

d. T

his

allo

ws

the

cell

to b

ehav

e lik

e a

bran

d-ne

w e

mbr

yoni

c ce

ll ra

ther

than

a d

iffer

entia

ted

adul

t cel

l.

Usin

g th

e el

ectri

c sh

ock

tech

niqu

e, W

ilmut

and

Cam

pbel

l fu

sed

enuc

leat

ed sh

eep

egg

cells

w

ith u

dder

cel

ls fro

m a

fem

ale

adul

t sh

eep.

Of

277

atte

mpt

s, on

ly o

ne p

rodu

ced

an e

mbr

yo

that

was

car

ried

to t

erm

in

a su

rroga

te m

othe

r. Th

is fa

mou

s la

mb,

na

med

D

olly,

br

ough

t cl

onin

g in

to th

e lim

elig

ht.

Wha

t did

this

tell

us?

Dol

ly w

as

the

first

m

amm

al

ever

to

be

cl

oned

usin

g a

dono

r nu

cleu

s fro

m a

n ad

ult

som

atic

cel

l. H

er

arriv

al

brou

ght

the

pote

ntia

l im

plic

atio

ns

for

clon

ing,

es

peci

ally

co

ntro

vers

ies

over

cl

onin

g hu

man

s an

d st

em c

ell

rese

arch

, int

o th

e pu

blic

eye

.

Prom

ises

and

Pit

falls

of H

uman

Clo

ning

Wil

liam

Jeff

erso

n C

lint

on

U.S.

Pr

esid

ent

Clin

ton

bloc

ked

fede

ral

fund

ing

for

hum

an

clon

ing

rese

arch

.

Afte

r Dol

ly th

e sh

eep

was

clon

ed

in

1996

, C

linto

n te

mpo

raril

y re

stric

ted

the

use

of t

axpa

yer

fund

s to

sup

port

rese

arch

on

hum

an c

loni

ng.

He

also

ask

ed

the

Nat

iona

l Bio

ethi

cs A

dviso

ry

Cou

ncil (

NBA

C) t

o as

sess

hum

an

clon

ing

rese

arch

. Th

e N

BAC

co

nclu

ded

that

an

y at

tem

pt

to

clon

e hu

man

s by

nu

clea

r tra

nsfe

r is

an

“irre

spon

sible

, un

ethi

cal,

and

unpr

ofes

siona

l ac

t” b

ut re

com

men

ded

that

any

la

ws b

e te

mpo

rary

and

revi

ewed

ag

ain

in s

ever

al y

ears

. Whi

le th

e N

BAC

re

ques

ted

that

pr

ivat

e or

gani

zatio

ns a

lso d

elay

clo

ning

rese

arch

, sev

eral

of

thes

e w

ent

ahea

d w

ith th

eir r

esea

rch

plan

s.Ba

sed

on

the

NBA

C

reco

mm

enda

tions

, C

linto

n en

cour

aged

Con

gres

s to

pas

s a

law

ban

ning

hum

an re

prod

uctiv

e cl

onin

g in

th

e U

nite

d St

ates

. H

owev

er, h

e su

ppor

ted

clon

ing

rese

arch

th

at

coul

d le

ad

to

signi

fican

t m

edic

al

bene

fits

incl

udin

g th

erap

eutic

clo

ning

to

crea

te h

uman

em

bryo

nic

stem

ce

lls fo

r res

earc

h.W

hat

did

this

tell

us?

Clo

ning

pr

oced

ures

ca

n be

us

ed

for

diffe

rent

res

ults

. D

uplic

atin

g a

hum

an

usin

g cl

onin

g cr

eate

s m

any

ethi

cal

prob

lem

s. H

owev

er, u

sing

clon

ing

to cr

eate

ce

lls a

nd ti

ssue

s to

treat

illn

esse

s m

ight

be

bene

ficia

l.

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Don

or c

ell f

rom

udd

er

cells

in a

dult

she

ep

Nuc

leus

rem

oved

from

egg

cel

l

Udd

er c

ell n

ucle

us

mov

es in

to e

gg c

ell

Elec

tric

sho

ck f

uses

ud

der

cell

wit

h eg

g ce

ll

Cell

grow

s in

to

shee

p cl

one

Page 10: You’ve Come A Long Way, Dolly! · 2020-08-11 · • Classroom set of Mock Newspaper Articles (cut each page in half along dotted line) • Student handouts • Tape • Stickers

GN

Tech

niqu

e

Mon

key

See,

Mon

key

Do,

Mon

key

Mon

key

Clon

ed A

s Tw

oLi

Men

g, J

ohn

Ely,

Ric

hard

Sto

uffer

, and

Don

Wol

f

Mon

keys

cl

oned

by

em

bryo

nic

cell

nucl

ear

trans

fer.

Prim

ates

ar

e go

od

mod

els

for

stud

ying

hu

man

ge

netic

di

sord

ers.

Clo

ning

id

entic

al

prim

ates

w

ould

de

crea

se

the

gene

tic v

aria

tion

and

num

ber

of a

nim

als

in r

esea

rch

stud

ies

rela

ted

to

hum

an

gene

tic

cond

ition

s.Si

mila

r to

pr

evio

us

clon

ing

expe

rimen

ts,

Wol

f’s

team

of

sc

ient

ists

fuse

d ea

rly-s

tage

em

bryo

nic

cells

with

enu

clea

ted

mon

key

egg

cells

usin

g a

smal

l el

ectri

cal

shoc

k. T

he r

esul

ting

embr

yos

wer

e th

en i

mpl

ante

d

into

su

rroga

te

mot

hers

. O

ut

of

29

clon

ed

embr

yos,

two

mon

keys

wer

e bo

rn.

One

was

a

fem

ale

nam

ed N

eti,

and

the

othe

r was

a m

ale

nam

ed D

itto.

W

hat d

id th

is te

ll us

? P

rimat

es,

whi

ch

are

hum

ans’

clos

est

rela

tives

, can

be

clon

ed.

Follo

win

g D

olly

, Pha

rmin

g Po

llyA

ngel

ika

Schn

ieke

, Kei

th C

ampb

ell,

and

Ian

Wil

mut

Tran

sgen

ic

shee

p cl

ones

ca

n pr

oduc

e tre

atm

ents

for

hum

an m

edic

al d

isea

se.

How

can

hum

ans

bene

fit f

rom

clo

ning

te

chno

logy

? W

hy w

ould

we

wan

t to

do

this?

Tran

sgen

ic t

echn

olog

y –

the

trans

fer

of

gene

s fro

m o

ne s

peci

es i

nto

anot

her

– w

as b

eing

refi

ned

just

as

mam

mal

ian

clon

ing

hit t

he li

mel

ight

. Thi

s te

chno

logy

m

akes

it p

ossib

le to

pro

duce

tran

sgen

ic

anim

als t

hat s

erve

as p

rodu

ctio

n fa

ctor

ies

for m

edic

ally

use

ful p

rote

ins.

One

con

veni

ent

way

to

prod

uce

larg

e qu

antit

ies

of

a tra

nsge

nic

prot

ein

is to

eng

inee

r an

ani

mal

to

prod

uce

the

prot

ein

in it

s m

ilk. S

impl

y by

milk

ing

the

anim

als,

we

can

colle

ct th

e pr

otei

n, p

urify

it

and

use

it fo

r med

ical

pur

pose

s.

Wilm

ut, C

ampb

ell a

nd S

chni

eke

set o

ut

to c

reat

e cl

oned

she

ep t

hat

expr

esse

d th

e hu

man

gen

e en

codi

ng t

he b

lood

cl

ottin

g Fa

ctor

IX

(“fac

tor

nine

”). T

his

prot

ein

can

be u

sed

to tr

eat p

eopl

e w

ith

hem

ophi

lia, a

diso

rder

that

resu

lts in

the

inab

ility

to st

op b

leed

ing

whe

n in

jure

d.

To

crea

te

the

trans

geni

c sh

eep,

th

e sc

ient

ists i

ntro

duce

d th

e hu

man

Fac

tor I

X ge

ne in

to t

he n

ucle

i of

shee

p sk

in c

ells

grow

n in

a la

bora

tory

dish

. The

se n

ucle

i w

ere

then

tran

sfer

red

to sh

eep

egg

cells

, cr

eatin

g tra

nsge

nic

clon

ed

embr

yos.

Polly

w

as

the

first

tra

nsge

nic

shee

p pr

oduc

ed th

is w

ay.

Wha

t di

d th

is te

ll us

? S

heep

can

be

gene

tical

ly

engi

neer

ed

to

prod

uce

ther

apeu

tic p

rote

ins

for

hum

ans

in th

eir

milk

. The

con

verg

ence

of t

rans

geni

c an

d cl

onin

g te

chno

logi

es r

esul

ted

in a

new

ap

proa

ch to

trea

ting

hum

an d

iseas

es.

Tech

niqu

eTh

e Fa

ctor

IX

gen

e is

in

sert

ed in

to c

ells

Nuc

leus

rem

oved

from

egg

cel

l

Cell

nucl

eus

(wit

h ge

ne)

mov

es t

o eg

g ce

ll

Elec

tric

sho

ck f

uses

cel

l (w

ith

gene

) wit

h eg

g ce

ll

Cell

grow

s in

to

shee

p w

ith

gene

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Don

or c

ell f

rom

mon

-ke

y em

bryo

cel

ls

Nuc

leus

rem

oved

from

egg

cel

l

Embr

yo c

ell n

ucle

us

mov

es in

to e

gg c

ell

Elec

tric

sho

ck f

uses

em

bryo

cel

l wit

h eg

g ce

ll

Cell

grow

s in

to

mon

key

clon

eFa

ctor

IX G

en

e

Page 11: You’ve Come A Long Way, Dolly! · 2020-08-11 · • Classroom set of Mock Newspaper Articles (cut each page in half along dotted line) • Student handouts • Tape • Stickers

AWTo

Clo

ne o

r Not

to C

one?

Ric

hard

See

d an

d Le

e B

o-ye

on

Pers

pect

ives

on

clon

ing

hum

ans

and

hum

an

clon

ing

rese

arch

.

Som

e pe

ople

supp

ort h

uman

cl

onin

g. F

or e

xam

ple,

Ric

hard

Se

ed i

s an

Am

eric

an p

hysic

ist

and

self-

proc

laim

ed

“ferti

lity

expe

rt.”

In 1

998

he a

nnou

nced

pl

ans

to c

lone

a h

uman

bei

ng

befo

re a

ny f

eder

al b

an c

ould

be

ena

cted

. Ar

ound

the

sam

e tim

e, S

outh

Kor

ean

rese

arch

er

Lee

Bo-y

eon

clai

med

to

have

us

ed n

ucle

ar t

rans

fer

with

an

enuc

leat

ed e

gg a

nd a

som

atic

ce

ll fro

m th

e sa

me

wom

an.

He

repo

rted

that

one

of

the

resu

lting

egg

s be

gan

divi

ding

. H

owev

er,

the

Kore

an r

esea

rch

team

ha

lted

its

deve

lopm

ent

at

the

four

-cel

l st

age,

be

fore

it

coul

d be

im

plan

ted

into

a

surro

gate

mot

her’s

wom

b. T

he

rese

arch

w

orld

re

ject

ed

Bo-

yeon

’s cl

aim

, de

man

ding

mor

e sc

ient

ific

evid

ence

.W

hat d

id th

is te

ll us

? Ad

vanc

es

in g

enet

ics

are

pres

entin

g th

e w

orld

w

ith

new

ch

oice

s. Th

e po

tent

ial

for

hum

an

clon

ing

exist

s an

d m

ay s

oon

beco

me

a re

ality

. So

me

peop

le

supp

ort

rese

arch

ing

this

pote

ntia

l.

Dol

ly S

how

s Fo

lks

How

to C

lone

a M

ouse

Teru

hiko

Wak

ayam

a an

d R

yuzo

Yan

agim

achi

Fem

ale

mou

se

clon

ed

by s

omat

ic c

ell

nucl

ear

trans

fer.

Dol

ly w

as c

lone

d us

ing

gene

tic

mat

eria

l fro

m

diffe

rent

iate

d ad

ult c

ells.

Can

oth

er m

amm

als

be c

lone

d th

is w

ay?

Wak

ayam

a an

d Ya

nagi

mac

hi

used

m

ouse

cu

mul

us

cells

as

nu

cleu

s do

nors

. Th

ese

diffe

rent

iate

d ce

lls n

ouris

h eg

g ce

lls in

fem

ale

adul

t m

ice.

Bu

t in

stea

d of

us

ing

an

elec

tric

shoc

k to

trans

fer t

he n

ucle

us, t

he

scie

ntist

s in

ject

ed t

he n

ucle

us

dire

ctly

into

the

egg

cell.

The

new

ce

ll w

as

chem

ical

ly

stim

ulat

ed

to

divi

de

and

impl

ante

d in

to

a su

rroga

te

mot

her

mou

se. T

he fi

rst c

lone

d m

ouse

pup

, na

med

Cum

ulin

a,

was

bor

n 19

day

s lat

er.

Wha

t di

d th

is te

ll us

? C

loni

ng

usin

g do

nor

nucl

ei f

rom

adu

lt ce

lls

can

be

perfo

rmed

in

m

amm

als o

ther

than

shee

p.

Tech

niqu

eC

umul

us c

ell e

xtra

cted

fr

om a

dult

fem

ale

mou

se

Nuc

leus

rem

oved

from

egg

cel

l

Cum

ulus

cel

l nuc

leus

in

ject

ed in

to e

gg c

ell

Cell

grow

s in

to

fem

ale

mou

se c

lone

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Lee

Bo-

yeon

Ric

hard

See

d

Page 12: You’ve Come A Long Way, Dolly! · 2020-08-11 · • Classroom set of Mock Newspaper Articles (cut each page in half along dotted line) • Student handouts • Tape • Stickers

DY

Tech

niqu

e

Fibr

o Br

ings

Mac

ho to

Cum

ulin

a’s A

rena

Teru

hiko

Wak

ayam

a an

d R

yuzo

Yan

agim

achi

Mal

e m

ouse

clo

ned

by

som

atic

ce

ll nu

clea

r tra

nsfe

r.

Up

to t

his

poin

t, al

l su

cces

sful

ad

ult c

ell c

loni

ng a

ttem

pts

used

ce

lls a

ssoc

iate

d w

ith t

he fe

mal

e re

prod

uctiv

e sy

stem

.

Can

a m

ale-

deriv

ed a

dult

cell

be

used

to g

ener

ate

a m

ale

mou

se?

Wak

ayam

a an

d Ya

nagi

mac

hi

isola

ted

dono

r nu

clei

fro

m c

ells

colle

cted

fro

m t

he t

ail

tips

of

adul

t m

ale

mic

e. A

fter

inje

ctin

g th

ese

nucl

ei in

to e

nucl

eate

d eg

g ce

lls, th

e sc

ient

ists t

rans

ferre

d th

e re

sulti

ng e

mbr

yos

into

sur

roga

te

mot

her m

ice.

The

sing

le re

sulti

ng

mal

e pu

p, n

amed

Fib

ro, w

as th

e on

ly

succ

essf

ul

clon

e in

27

4 at

tem

pts.

Wha

t di

d th

is te

ll us

? C

loni

ng

usin

g ad

ult

som

atic

cel

ls isn

’t re

stric

ted

to f

emal

es o

r to

cel

ls as

soci

ated

with

the

repr

oduc

tive

syst

em.

Infe

rtili

ty D

rive

s Cl

onin

g R

esea

rch

Seve

rino

Ant

inor

i and

Pan

ayio

tis

Zav

os

Hum

an

clon

ing

mig

ht

solv

e pr

oble

ms

of

coup

les

havi

ng d

ifficu

lty

in b

ecom

ing

preg

nant

.

A gr

oup

of re

prod

uctiv

e ex

perts

an

noun

ced

thei

r pla

n to

clo

ne a

hu

man

with

in th

e ne

xt tw

o ye

ars.

This

inte

rnat

iona

l gro

up in

clud

es

Gre

ek-A

mer

ican

re

sear

cher

Pa

nayi

otis

Zavo

s an

d Ita

lian

rese

arch

er

Seve

rino

Antin

ori.

Zavo

s an

d ot

her

scie

ntist

s ha

ve

argu

ed t

he b

enefi

ts o

f hu

man

cl

onin

g re

sear

ch i

n fro

nt o

f a

U.S.

Con

gres

siona

l Co

mm

ittee

fo

rmed

to

disc

uss

issue

s ra

ised

by

hum

an

clon

ing.

Antin

ori

anno

unce

d th

at a

wom

an w

ould

gi

ve b

irth

to a

clo

ned

baby

in

Janu

ary

2003

, bu

t pr

ovid

ed

no

scie

ntifi

c de

tails

fo

r th

e pr

egna

ncy.

M

any

scie

ntifi

c ex

perts

are

ske

ptic

al a

bout

his

clai

m

of

achi

evin

g a

hum

an

clon

e.

Wha

t did

this

tell

us?

Not

bei

ng

able

to

have

a b

aby

caus

es a

lo

t of

em

otio

nal

pain

in

som

e pe

ople

’s liv

es.

Scie

ntist

s ar

e lo

okin

g at

clo

ning

as

a po

ssib

le

solu

tion

for i

nfer

tility

.

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Nuc

leus

rem

oved

from

egg

cel

l

Tail

cell

nucl

eus

in

ject

ed in

to e

gg c

ell

Tail

tip

cell

extr

acte

d fr

om a

dult

mal

e m

ouse

Cell

grow

s in

to m

ale

mou

se c

lone

Seve

rino

Ant

inor

iPa

nayi

otis

Zav

os

Page 13: You’ve Come A Long Way, Dolly! · 2020-08-11 · • Classroom set of Mock Newspaper Articles (cut each page in half along dotted line) • Student handouts • Tape • Stickers

LO

Tech

niqu

e

Hum

an C

loni

ng to

Bol

ster

Ste

m C

ell Th

erap

ies?

Adv

ance

d Ce

ll Te

chno

logy

Hum

an c

loni

ng m

ight

als

o be

use

d to

cre

ate

stem

cel

ls

for n

ew m

edic

al tr

eatm

ents

.

How

mig

ht c

loni

ng a

hum

an b

enefi

t m

edic

al re

sear

ch?

Early

em

bryo

s are

com

pose

d of

st

em c

ells,

whi

ch c

an b

ecom

e an

y ki

nd o

f ce

ll in

the

hum

an b

ody.

A po

tent

ial

appr

oach

to

re

pairi

ng

tissu

e da

mag

e in

a p

atie

nt’s

body

is

by u

sing

the

patie

nt’s

gene

tic

mat

eria

l to

clo

ne a

n ea

rly e

mbr

yo

that

wou

ld b

e sp

lit i

nto

indi

vidu

al

stem

cel

ls.

Thes

e ce

lls w

ould

be

grow

n in

the

la

bora

tory

, pr

oduc

ing

mat

chin

g tis

sue

to r

epai

r th

e da

mag

e. T

his

appr

oach

, ca

lled

ther

apeu

tic

clon

ing,

di

ffers

from

rep

rodu

ctiv

e cl

onin

g in

tha

t it

aim

s to

pro

duce

cel

ls an

d tis

sues

ra

ther

than

a co

mpl

ete

hum

an b

eing

.

Scie

ntist

s at

Ad

vanc

ed

Cel

l Te

chno

logy

, an

Am

eric

an

biot

echn

olog

y co

mpa

ny, c

lone

d th

e fir

st d

ocum

ente

d hu

man

em

bryo

by

nucl

ear

trans

fer,

usin

g th

e nu

cleu

s fro

m

an

adul

t cu

mul

us

cell.

Th

e cl

oned

egg

dev

elop

ed i

nto

a six

-ce

lled

embr

yo b

efor

e it

stop

ped

grow

ing.

Thi

s ex

perim

ent

show

ed

that

ther

apeu

tic c

loni

ng m

ight

be

a re

alist

ic a

ppro

ach

to p

rodu

cing

stem

ce

lls fo

r med

ical

pur

pose

s.

Wha

t di

d th

is te

ll us

? Th

e en

d re

sult

of c

loni

ng is

n’t

alw

ays

a fu

lly

deve

lope

d or

gani

sm. C

loni

ng m

ight

al

so b

e us

ed to

cre

ate

stem

cel

ls fo

r ne

w m

edic

al th

erap

ies.

New

Pre

side

nt, N

ew P

olic

ies

Geo

rge

Wal

ker

Bus

h

U.S.

Pre

side

nt B

ush

bans

fe

dera

l fu

ndin

g of

al

l hu

man

clo

ning

rese

arch

.Pr

esid

ent

Bush

cr

eate

d th

e Pr

esid

ent’s

Cou

ncil

on B

ioet

hics

. Th

is gr

oup

carr

ied

out a

miss

ion

simila

r to

fo

rmer

Pr

esid

ent

Clin

ton’

s N

atio

nal

Bioe

thic

s Ad

viso

ry C

ounc

il.Th

e Bu

sh

adm

inist

ratio

n pr

ohib

ited

taxp

ayer

fu

ndin

g to

su

ppor

t re

sear

ch

invo

lvin

g th

e cl

onin

g an

d de

stru

ctio

n of

hu

man

em

bryo

s. H

e al

so

supp

orte

d a

fede

ral b

an o

n bo

th

hum

an re

prod

uctiv

e cl

onin

g an

d th

erap

eutic

cl

onin

g to

cr

eate

st

em c

ells

for r

esea

rch.

Wha

t do

es

this

tell

us?

Not

ev

eryo

ne d

iffer

entia

tes b

etw

een

the

two

type

s of c

loni

ng.

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Nuc

leus

rem

oved

from

egg

cel

l

Cum

ulus

cel

l ext

ract

ed

from

adu

lt fe

mal

e hu

man

Cell

divi

des

to fo

rm

a fo

ur-c

elle

d em

bryo

Embr

yo c

ells

spl

it t

o fo

rm s

tem

cel

l lin

es

Cum

ulus

cel

l nuc

leus

in

ject

ed in

to e

gg c

ell

Page 14: You’ve Come A Long Way, Dolly! · 2020-08-11 · • Classroom set of Mock Newspaper Articles (cut each page in half along dotted line) • Student handouts • Tape • Stickers

Y!L

Supe

rnat

ural

Bel

ief i

n Cl

onin

gH

uman

Clo

ning

Adv

ocat

es

Diff

eren

t per

spec

tives

on

hum

an

clon

ing

and

its

pote

ntia

l.Th

e Ra

elia

n M

ovem

ent

is a

relig

ious

sec

t w

hose

mem

bers

be

lieve

tha

t hu

man

s ar

e cl

ones

cr

eate

d by

alie

ns.

In 1

997,

the

Ra

elia

ns o

rgan

ized

Clo

naid

, “th

e fir

st h

uman

clo

ning

com

pany

.” Th

en in

200

2, th

e Ko

rean

offi

ce o

f C

lona

id cl

aim

ed to

hav

e a w

oman

pr

egna

nt w

ith a

clo

ned

embr

yo.

How

ever

, So

uth

Kore

an o

ffici

als

foun

d no

evi

denc

e su

ppor

ting

the

repo

rt. T

hey

rush

ed to

ena

ct

a go

vern

men

t ban

on

all h

uman

cl

onin

g in

the

coun

try.

Wha

t di

d th

is te

ll us

? D

iffer

ent

grou

ps o

f pe

ople

hol

d di

ffere

nt

belie

fs a

nd v

alue

s. It’

s im

porta

nt

to a

naly

ze a

ll sid

es o

f an

iss

ue

befo

re

mak

ing

your

ow

n de

cisio

ns.

Also

, th

e hu

man

cl

onin

g iss

ue

exte

nds

wel

l be

yond

the

bord

ers o

f the

Uni

ted

Stat

es, i

nflue

ncin

g ci

tizen

s of

all

coun

tries

.

Hou

se S

uppo

rts

Clon

ing

Ban

Geo

rge

Wal

ker

Bus

h

Hum

an

clon

ing

bill

pass

ed in

the

U.S.

Hou

se

of R

epre

sent

ativ

es.

The

U.

S.

Hou

se

of

Repr

esen

tativ

es p

asse

d a

ban

agai

nst

both

rep

rodu

ctiv

e an

d th

erap

eutic

hum

an c

loni

ng i

n 20

02.

The

bill

was

the

n se

nt

to t

he S

enat

e, t

he o

ther

hal

f of

C

ongr

ess,

for a

vot

e.

Wha

t doe

s th

is te

ll us

? If

such

a

bill

pass

ed, s

cien

tists

fund

ed b

y ta

xpay

er m

oney

can

be p

ut in

jail

if th

ey d

o an

y cl

onin

g re

sear

ch.

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Th

e C

lon

ing

Tim

esT

he

Clo

nin

g T

imes

Rae

l wit

h m

odel

of a

lien

spac

eshi

p