vibrio abundance in tidal creeks: sediment/water...
TRANSCRIPT
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M A R I E E N G L I S H U N C I N S T I T U T E O F M A R I N E S C I E N C E S M E N T O R S : D R . R A C H E L T . N O B L E A N D
D R . B R E T T F R O E L I C H
VIBRIO ABUNDANCE IN TIDAL CREEKS: SEDIMENT/WATER DYNAMICS
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THE GENUS VIBRIO
• Native to estuaries (Nearly 35% of coastal bacterial pop.) • Facultative anaerobes • Pathogenic spp. • Vibrio cholerae, Vibrio vulnificus, Vibrio parahaemolyticus • V. vulnificus is most important seafood-borne pathogen in the USA
• Can cause wound infections, septicemia, gastroenteritis • Recent research shows climate change is leading to an
increased # of vibrio cases (Oliver, DUML seminar)
• Ecology • Influenced mainly by salinity and temperature • Halophilic • Predictive models for vibrio sp. concentrations based on salinity and
temp are 70-75% accurate (Hsieh et al., 2007)
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SALINITY/TEMP RANGE FOR PATHOGENIC VIBRIO SP.
• Optimal salinity range • 5-25 ppt • Very low and very high salinities appear to be detrimental to
growth and persistence (Noble, 2008)
• Optimal water temp. range • 20-30 ⁰C is where the highest concentrations are seen • Below 20 ⁰C = low density of vibrio • Below 10 ⁰C = no vibrio in water column
Parameter Range Mean and S.D.
Salinity (ppt) 15.00 - 32.00 26.05 ± 5.875
Temperature (⁰C) 15.41 - 30.32 22.67 ± 4.316
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“The Biology of Vibrios”
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STUDY SITES
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INDIVIDUAL SITES
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Site 1 Site 2
Site 4 Site 3
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PART OF THE LOCAL COMMUNITY
Locals fish for shrimp, flounder, and mullet
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CALICO CREEK SAMPLING
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CALICO CREEK SAMPLING
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PROCESSING
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TOTAL VIBRIO ENUMERATION
10/5
CFU= colony forming units
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CHROM AGAR
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10/5
Purple colonies= V. parahaemolyticus Blue colonies= V. vulnificus
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WHAT CAUSES THE DECLINE IN VIBRIO ABUNDANCE IN THE WATER COLUMN?
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WHAT CAUSES THE DECLINE IN VIBRIO ABUNDANCE IN THE WATER COLUMN?
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BACTERIA IN THE WATER COLUMN DECREASE WHEN CONDITIONS ARE STRESSFUL
Low temperatures = Stressful for bacteria
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POST-HURRICANE SANDY = COOLER WATER TEMPS
11/1
1 2 3 4 Sed. (1:10) Water (1:10) Water (1:1)
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BACTERIA IN THE WATER COLUMN DECREASE WHEN CONDITIONS ARE STRESSFUL
High salinities = stressful for bacteria
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VIBRIO SP. ABUNDANCE IS HIGHER IN SEDIMENTS
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SEDIMENT > WATER
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2S 1S 2W 1W
4S 3S 4W 3W
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ARE SEDIMENT ABUNDANCES AFFECTED BY STRESSFUL CONDITIONS?
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SEDIMENT PROTECTION
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SEDIMENT PROTECTION
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ARE SEDIMENT ABUNDANCES AFFECTED BY STRESSFUL CONDITIONS?
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IS THIS UNIQUE TO VIBRIO, AN AUTOCHTHONOUS BACTERIA?
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IS THIS UNIQUE TO VIBRIO, AN AUTOCHTHONOUS BACTERIA?
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FUTURE STUDIES • Continue study in colder months using qPCR to
compare with cultured methods for signs of overwintering (VBNC cells) • Must use molecular methods below 15 ⁰C because the cells
enter a state where they are no longer culturable
• Organic matter content of sediments and correlation with pathogenic Vibrio • Grain size analysis of sediments and correlation with
pathogenic Vibrio
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CONCLUSIONS
1. In harsh conditions, Vibrio decrease in the water column. 2. Bacteria are found in higher concentrations in the sediments 3. Vibrio in the sediments are somewhat protected from salinity
and temperature changes in water column 4. The sediments provide protection for endogenous bacteria,
but the same pattern was not seen for exogenous (the control).
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ACKNOWLEDGEMENTS
Rachel Noble Brett Froelich Sarah Rhodes
Sydney Brothers Denene Blackwood
Monica Greene Raul Gonzalez
Mark Vergougstraete Rodney Guajardo
Sara Posey
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BIBLIOGRAPHY
• DUML Seminar with Dr. Jim Oliver: "Vibrio vulnificus: Death on the Half-Shell” Wednesday, August 29, 201212:30pm, LSRC A328 & Marine Lab: Repass Center
• Thompson, Fabiano Lopes, Brian Austin, and J. G. Swings. "12: Aquatic Environment." The Biology of Vibrios. Washington, D.C.: ASM, 2006. 176. Print.
• Hsieh, Jennifer L., J. Stephen Fries, and Rachel T. Noble. "Dynamics and Predictive Modelling of Vibrio Spp. in the Neuse River Estuary, North Carolina, USA." Environmental Microbiology 0.0 (2007): 070907020408001-??? Print.