thermo-forma 3110 seriesi operating manual-1

Forma Scientific DivisionMillcreek Road, P.O. Box 649Marietta, Ohio 45750U.S.A.

Telephone: (740) 373-4763Telefax: (740) 373-4189

________________________________________

Models: 3110 Series*Single Chamber

Water Jacket Incubator

Models: 3210 Series*Dual Chamber

Water Jacket Incubator

Operating and MaintenanceManual

Manual No: 7023110 Rev. 4

Read this Instruction Manual

Failure to read, understand and follow the instructions in this manual may result in damage to theunit, injury to operating personnel and poor equipment performance.

CAUTION: All internal adjustments andmaintenance must be performedby qualified service personnel.

Refer to the serial tag on therear of this instruction manual

Forma Model 3100/3200 Series ___________________________________________

ii

The material in this manual is for information purposes only. The contents and the product it describesare subject to change without notice. ThermoQuest makes no representations or warranties with respect tothis manual. In no event shall ThermoQuest or Forma Scientific Division be held liable for any damages,direct or incidental, arising out of or related to the use of this manual.

MANUAL NO. 7023110

-- 18766/IN-2763 12/13/99 Removed RH SNSR ERR alarm ccs-- Per J. Lang 11/29/99 Added steps to remove and install cover plate in Section 5.7 ccs4 18614 10/25/99 Clarified Section 5.4.a for door and hinge alignment ccs3 18118/IN-2701 5/25/99 Updated vinyl plugs to nylon screws in door reverse ccp2 18141/IN-2686 3/25/99 Added drain plug (110084) artwork, instructions and to parts list ccp

REV ECR/ECN DATE DESCRIPTION By


iii

Single Chamber ModelsModel CO2 Sensor** O2 Voltage***

3110 T/C No 1153111 T/C No 2303120 IR No 1153121 IR No 2303130 T/C Yes 1153131 T/C Yes 2303140 IR Yes 1153141 IR Yes 230

Dual Chamber ModelsModel* CO2 Sensor** O2 Voltage***

3210 T/C No 1153211 T/C No 2303220 IR No 1153221 IR No 2303230 T/C Yes 1153231 T/C Yes 2303240 IR Yes 1153241 IR Yes 230

*The models listed as dual chamber models are stacked or dual chamber units. These unitsarrive as single stand-alone units. They may be stacked one on top of the other or configuredside by side with opposite door swing.

**T/C is a thermal conductivity sensor. IR is an infrared sensor.

***All units are 50/60 HZ.


iv

General Safety Notes used in this Manual

Important operating and/or maintenance instructions. Read the accompanyingtext carefully.

Ce symbole attire lattention de lutilisateur sur des instructions importantes defonctionnement et/ou dentretien. Il peut tre utilis seul ou avec dautres symboles descurit. Lire attentivement le texte daccompagnement.

Wichtige Betriebs- und/oder Wartungshinweise. Lesen Sie den nachfolgenden Textsorgfltig.

Importante instruccions de operacion y/o mantenimiento. Lea el texto acompanantecuidadosamente.

Potential electrical hazards. Only qualified persons should perform proceduresassociated with this symbol.

Ce symbole attire lattention de lutilisateur sur des risques lectriques potentiels.Seules des personnes qualifies doivent appliquer les instructions et les procduresassocies ce symbole.

Gefahr von Stromschlgen. Nur qualifizierte Personen sollten die Ttigkeitenausfhren, die mit diesem Symbol bezeichnet sind.

Potencial de riesgos electricos. Solo personas das capacitadadas deben ejecutar losprocedimientos asociadas con este simbulo.

Equipment being maintained or serviced must be turned off and locked off toprevent possible injury.

Risques potentiels lis lnergie. Lquipement en entretien ou en maintenance doittre teint et mis sous cl pour viter des blessures possibles.

Gerte, an denen Wartungs- oder Servicearbeiten durchgefhrt werden, mssenabgeschaltet und abgeschlossen werden, um Verletzungen zu vermeiden.

El equipo recibiendo servicio o mantenimiento debe ser apagado y segurado paraprevenir danos.

Forma Model 3100/3200 Series_______________________________________Safety

v

Hot surface(s) present which may cause burns to unprotected skin or tomaterials which may be damaged by elevated temperatures.

Prsence de surface(s) chaude(s) pouvant causer des brlures sur la peau nonprotge, ou sur des matires pouvant tre endommages par des tempraturesleves.

Heie Oberflche(n) knnen ungeschtzter Haut Verbrennungen zufgen oderSchden an Materialien verursachen, die nicht hitzebestndig sind.

Superficias calientes que pueden causar quemaduras a piel sin proteccion o amateriales que pueden estar danados por elevadas temperaturas.

Always use the proper protective equipment (clothing, gloves, goggles etc.). Always dissipate extreme cold or heat and wear protective clothing. Always follow good hygiene practices. Each individual is responsible for his or her own safety.


vi

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do, please contact us 8:00 a.m. to 7:00 p.m. (Eastern Time) at:

1-740-373-4763 Direct1-888-213-1790 Toll Free, U.S. and Canada1-740-373-4189 FAXhttp://www.forma.com Internet Worldwide Web Home [email protected] Service E-Mail Address

If you

Our staff can provide information on pricing and give you quotations. We can take your order and provide delivery information on major equipment items or make arrangements to have your local sales representative contact you. Our products are listed on the Internet and we can be contacted through our Internet home page.

Our staff can supply technical information about proper setup, operation or troubleshooting of your equipment. We can fill your needs for spare or replacement parts or provide you with on-site service. We can also provide you with a quotation on our Extended Warranty for your Forma products.

Whatever Forma products you need or use, we will be happy to discuss your applications. If you are experiencing technical problems, working together, we will help you locate the problem and, chances are, correct it yourself...over the telephone without a service call.

When more extensive service is necessary, we will assist you with direct factory trained technicians or a qualified service organization for on-the-spot repair. If your service need is covered by the warranty, we will arrange for the unit to be repaired at our expense and to your satisfaction.

Regardless of your needs, our professional telephone technicians are available to assist you Monday through Friday from 8:00 a.m. to 7:00 p.m. Eastern Time. Please contact us by telephone or fax. If you wish to write, our mailing address is:

ThermoQuestForma Scientific DivisionPO Box 649Marietta, OH 45750

International customers please contact your local ThermoQuest, Forma Scientific Division distributor.

Sales Support

Service Support

Forma Model 3100/3200 Series ________________________________________Contents

viii

Table of Contents

Section 1 - Installation and Start-Up1.1 Name and Description of Parts ................................................................... 1-11.2 Control Panel Keys, Displays and Indicators.............................................. 1-21.3 Operating the Control Panel........................................................................ 1-31.4 Displays....................................................................................................... 1-51.5 Installing the Unit........................................................................................ 1-6

a. Choosing the Location.......................................................................... 1-6b. Stacking the Incubators ......................................................................... 1-6c. Preliminary Cleaning and Disinfecting.................................................. 1-9d. Installing the Shelves............................................................................. 1-9e. Installing the Access Port Stopper ......................................................... 1-10f. Installing the HEPA Filter...................................................................... 1-10g. Leveling the Unit ................................................................................... 1-12h. Connecting the Unit to Electrical Power ............................................... 1-12i. Filling the Water Jacket.......................................................................... 1-12j. Filling the Humidity Pan ........................................................................ 1-13k. Connecting the CO2 Gas Supply ........................................................... 1-14l. Connecting the N2 Gas Supply............................................................... 1-15

1.6 Incubator Start-Up....................................................................................... 1-15a. Setting the Operating Temperature........................................................ 1-16b. Setting the Overtemp Setpoint .............................................................. 1-16c. Setting the CO2 Setpoint........................................................................ 1-17d. Setting the O2 Setpoint .......................................................................... 1-17

Chart 1-1, Set Mode........................................................................................... 1-18

Forma Model 3100/3200 Series _____________________________________Contents

ix

Section 2 - Calibration2.1 Calibrate Mode............................................................................................ 2-1

a. Calibrating the Temperature .................................................................. 2-1Temperature Stabilization Periods ............................................................. 2-1b. Calibrating the Thermal Conductivity CO2 System .............................. 2-2c. Calibrating the T/C %CO2 Zero ............................................................ 2-2 T/C CO2 Sensor Stabilization Periods....................................................... 2-3d. Calibrating the T/C %CO2 Span............................................................ 2-3e. Calibrating the Infra-Red CO2 System .................................................. 2-4 IR CO2 Sensor Stabilization Times ........................................................... 2-4f. Calibrating the O2 System ..................................................................... 2-4

O2 Sensor Stabilization Times.............................................................. 2-5g. Calibrating the Relative Humidity......................................................... 2-5 Relative Humidity Stabilization Times .................................................... 2-5Chart 2-1, Calibrate Mode.......................................................................... 2-7

Section 3 - Configuration3.1 Configuration Mode .................................................................................... 3-1

a. Turning the Audible Alarm ON/OFF .................................................... 3-1b. Setting an Access Code ......................................................................... 3-1c. Setting a Low Temp Alarm Limit (tracking alarm)............................... 3-2d. Setting a High Temp Alarm Limit (tracking alarm).............................. 3-2e. Enabling Temp Alarms to Trip Contacts............................................... 3-2f. Setting a Low CO2 Alarm Limit (tracking alarm).................................. 3-3g. Setting a High CO2 Alarm Limit (tracking alarm) ................................ 3-3h. Enabling CO2 Alarms to Trip Contacts ................................................. 3-3i. Setting a New Zero Number for T/C CO2 Sensors ................................ 3-3j. Setting a New Span Number for T/C CO2 Sensors ................................ 3-4k. Setting a Low RH Alarm Limit ............................................................. 3-4l. Enabling RH Alarms to Trip Contacts ................................................... 3-4m. New O2 Cell ......................................................................................... 3-5n. Setting a Low O2 Alarm Limit (tracking alarm).................................... 3-5o. Setting a High O2 Alarm Limit (tracking alarm)................................... 3-5p. Enabling O2 Alarms to Trip Contacts .................................................... 3-6q. Enabling Temp/RH to be Displayed...................................................... 3-6r. Enabling CO2/O2 to be Displayed .......................................................... 3-6


x

s. Enabling O2 Compensation for T/C CO2 Systems................................. 3-7t. Setting a RS-485 Communications Address (1535 compatible only).... 3-7u. Selecting a Primary Tank with the Gas Guard Option .......................... 3-7v. Enabling the Gas Guard System............................................................ 3-7w. Setting the Door Heat ........................................................................... 3-8Chart 3-1, Configure Mode (4 pages)......................................................... 3-9

Section 4 - Alarms4.1 Alarms (chart of alarms) ............................................................................. 4-14.2 Sensor Fault Alarms.................................................................................... 4-24.3 REPL O2 SNSR (Alarm) ............................................................................ 4-24.4 REPL IR SNSR (Alarm) ............................................................................. 4-24.5 CO2 SENS ERR ......................................................................................... 4-24.6 IR AUTO Z ERR ........................................................................................ 4-3

Section 5 - Maintenance5.1 Disinfecting the Incubator Interior.............................................................. 5-15.2 Cleaning the Cabinet Exterior..................................................................... 5-25.3 Cleaning the Humidity Pan ......................................................................... 5-25.4 Reversing the Door Swing .......................................................................... 5-3

a. Reversing the Hinges for the Exterior Door .......................................... 5-35.5 Replacing the Fuses .................................................................................... 5-85.6 HEPA Filter Maintenance........................................................................... 5-105.7 Draining Water Jacket................................................................................. 5-11

Section 6 - Factory Installed Options6.1 Connections to External Equipment ........................................................... 6-1

a. Connecting the Remote Alarm Contacts ............................................... 6-1b. Connecting the RS485 Interface, P/N 190523....................................... 6-2c. Connecting the Analog Output Boards.................................................. 6-2

6.2 Gas Guard for CO2 and N2 (190640/190642) ............................................. 6-4a. Connecting the CO2 Gas Supplies ......................................................... 6-5b. Connecting the N2 Gas Supplies ........................................................... 6-5c. Activating the Built-in Gas Guard......................................................... 6-6d. Operation of the CO2 or N2 Gas Guard ................................................. 6-6

6.3 Humidity Readout ........................................................................................ 6-7


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a. Factors Affecting the Humidity Level in the Chamber.......................... 6-7b. Accuracy of the Humidity Readout ....................................................... 6-8

6.4 Factory Installed Cooling Coil ..................................................................... 6-8a. Installing the Cooling Coil Incubator .................................................... 6-10

Section 7 - Specifications

Section 8 - Spare Parts

Section 9 - Electrical Schematics

Section 10 - Warranty Information

Appendix A

Forma Model 3100/3200 Series _________________________________Installation/Start-up

1-1

Section 1 - Installation and Start-up

1.1 Name and Description of Parts

Figure 1-1Water Jacket Incubator

Outer Door - Reversible to opposite swing,see Section 5.4

Heated Inner Door - Keeps chamberinterior dry. Reversible to opposite swing,see Section 5.4.

Chamber Gas Sample Port - Used forsampling chamber CO2 content, using aFyrite or similar instrument.

Note: The port must remaincapped when not in use.

Main Power Switch

Control Panel - Keypad,Displays and Indicators.(See Figure 1-2).

Fill Port - Used for filling thewater jacket.

Water Jacket Vent- Do notcover! Allows air to escapefrom the water jacket duringfilling and normal expansionand contraction when theincubator heats or cools.

Leveling Legs - Used tolevel the unit.

Water Jacket Drain Remove plug and usehose barb insert included. Plug when not inuse.

Note: The incubators are stackable.See Section 1.5.

*When setting up the incubator, install the coverplate packed inside the incubator shippingcarton .

Heated Glass

Inner Door

Leveling

Legs (4)

Jacket Vent

Fill Port

Jacket

Drain

(remove plug

before using)*

Control Panel

Chamber Gas

Sample Port

Power

Switch

Hose

Barb

Insert

Forma Model 3100/3200 Series _________________________________Installation/Start-up

1-2

1.2 Control Panel Keys, Displays and Indicators

SILENCE - Silences the audible alarm.

Alarm Indicator - Pulses on/off during analarm condition in the cabinet.

MODE Select Switch - Used to selectRun, Setpoints, Calibration and SystemConfiguration Modes.

Message Center - Displays system status.

Mode Select Indicators-Run: Run MenuSet: Set Points MenuCal: Calibrate MenuConfig: Configuration Menu

Up and Down Arrows: Increases ordecreases the parameter values that arenumbers, toggles the parameter values that arechoices.

Heat Indicator: Lights when power isapplied to the heaters.

Temp Display: Can be programmed todisplay temperature continuously, RHcontinuously (with RH option), or togglebetween temperature and humidity (with RHoption). See Section 3.1, Configuration.

Scroll for Parameters Keys: Scrolls theoperator through the parameters of the modethat is selected.

Figure 1-2 Control Panel

Forma Scientific, Inc. _____________________________________________Installation/Start-up

1-3

Inject Indicator: Lights when gas is being injected into the incubator. If the %CO2/O2 display (itemL) is continuously displaying CO2, the light indicates CO2 injection only. If the %CO2/O2 display iscontinuously displaying O2, light indicates N2 injection. If the %CO2/O2 is toggling, either a CO2 or N2injection will cause the indicator to light.

%CO2/O2 display: Can be programmed to display CO2 continuously or O2 continuously (on unitsequipped with O2), or toggle between CO2 and O2 (on units equipped with O2) See Section 3.1,Configuration.

1.3 Operating the Control Panel

The Model 3110 Series water jacket incubator has four basic modes, which allowincubator setup. The modes are as follows: Run, Setpoints, Calibration and SystemConfiguration.

Run is the default mode that the incubator will normally be in during operation.

Set is used to enter system setpoints for incubator operation.

Cal is used to calibrate various system parameters to the customers satisfaction.

Config is the system configuration mode that allows for custom setup of various options.

The chart on the following page shows the selections under each of the modes.


1-4

RUN SETPOINT CALIBRATION CONFIGURATION

Default Mode Temperature Temp Offset AudibleOvertemp CO2 Zero Access CodeCO2 CO2 Span Temp Lo AlarmO2 IR CO2 Span Temp Hi Alarm

O2 Span Temp RelayRH Offset CO2 Lo Alarm

CO2 Hi AlarmCO2 RelayCO2 Z & S #sRH Lo AlarmRH RelayO2 Hi AlarmO2 Lo AlarmO2 RelayDisplay 1 SetupDisplay 2 SetupO2 CompensationRS485 AddressGas Guard SetupDoor Heat

**Base Unit Displays**Option Displays


1-5

Scroll for Parameters Keys: Steps the operator through theparameters of SET, CAL and CONFIG Modes. The right arrow goesto the next parameter, the left arrow returns to the previous parameter.

Up Arrow: Increases or toggles the parameter value that has beenselected in the SET, CAL, and CONFIG Modes.

Enter: Must press Enter key to save to memory all changed values.

Down Arrow: Decreases or toggles the parameter values that have beenselected in the SET, CAL and CONFIG Modes.

Silence Key: Press to silence the audible alarm. See Section 4 for alarmringback times.

1.4 Displays

Message Center

Displays the system status (Mode) at all times. Displays SYSTEM OK during normal operation,or alarm messages if the system detects an alarm condition. See Section 4.1, Alarms.

Upper and Lower Displays

These 7-segment displays vary depending upon the options present and the configuration chosen.The upper display can display temp or RH, or toggle between them. The bottom display can displayCO2 or O2, or toggle between them. See Section 3.1, Configuration.


1-6

1.5 Installing the Unit

a. Choosing the Location

Locate the unit on a firm, level surface capable of supporting the units operational weight of365 lbs. (166 kg).

Locate away from doors and windows and heating and air conditioning ducts.

Allow enough clearance behind the unit for electrical and gas hook-up.

b. Stacking the Incubators

If the units have been in service, disconnect thepower cord connector and drain the water jacketof the designated top unit before stacking.

Note: Stacking brackets (shown at left) stacking bolts, washers, and bolts for stacking are included with each unit.

Figure 1-3 Stacking brackets

Designate one incubator tobe the top unit and the otheras the bottom unit. Removethe base cover plate from thetop unit using the finger holesin the base or using a slottedscrewdriver. (Figure 1-4)


1-7

Note the two slots in the base of theincubator which accommodate thestacking bolts. Refer to Figure 1-5.

Remove the two plastic plugs fromthe bolt holes in the exterior top ofthe bottom unit. Install the 1/2long 5/16-18 stacking bolts andwashers into the bolt holes - do nottighten the bolts at this time. Referto Figure 1-6

Unscrew and remove the leveling feetfrom the top unit and lift it onto thebottom unit, off-setting the base ofthe top unit approximately 2-3 inchesbehind the stacking bolts andwashers.

This incubator weighs 265 lbs (120kg) before filling. Havesufficient personnel to lift it.

Front of top Incubator, base cover plate removed

Slots for stacking bolts

Figure 1-5

5/16"-18 stacking bolt and washerFigure 1-6


1-8

Align the sides of the top unit with the bottomunit and slide the top unit forward until theslots in the base of the top unit align with the5/16 - 18 stacking bolts in the exterior top ofthe bottom unit. Refer to Figure 1-7.

Remove and save the two screws from theback of the control panel on the bottom unitas identified in Figure 1.8.

Insert the stacking brackets into the slots on the rear of the control panel of the bottom unit asshown in Figure 5-6. Align the slots in the brackets with the mounting holes on the rear of theincubators. Secure the brackets with the screws saved above and the 1/4-20 bolts provided inthe stacking kit. A 7/16 wrench or socket will be required for the bolts.

Secure the base of the top unit to the exterior top of the bottom unit by tightening the 5/16-18stacking bolts using a 1/2 (13mm) wrench or suitable tool.

Replace the base cover on the top unit.

The stacked units are ready to be placed into service.

(2) 5/16" bolts and flat washer

1/2" (13mm) wrench required

Figure 1-7

Back of top Incubator

Back of bottom Incubator

Figure 1-8

Insert 1/4-20 bolts

Remove this screw and install bracket

Remove this screw and install bracket

Stacking brackets Insert 1/4-20 bolts


1-9

c. Preliminary Cleaning and Disinfecting

Remove the protective plastic coating on the shelf supports and air duct, if present.

Using a suitable laboratory disinfectant, disinfect all interior surfaces including shelves and shelfsupports, door gaskets, blower wheel and CO2 sensor. Refer to Section 5.1.

d. Installing the Shelves

Install the shelf supports with the tabs facing into the center of the chamber with their slots up.There are no right side or left side supports, simply rotate one of them to fit the opposite side. Tiltthe shelf supports as they are placed in the chamber so the tops fit into the top air duct, then guidethem into the vertical position. Figure 1-9 shows the support as it would be oriented for the rightside of the chamber.

Referring to Figure 1-9, note that there are left side and right side shelf channels.

Right Shelf Channel

Left Shelf Channel

Shelf SupportFigure 1-9


1-10

Install the shelf channels by placingthe channels rear slot over theappropriate rear tab on the shelfsupport. Pull the shelf channelforward and engage the channelsfront slot into the shelf supportsappropriate forward tab. Refer toFigure 1-10.

Figure 1-11 shows one of the channelsinstalled on the right shelf support.

e. Installing the Access Port Stopper

Locate the opening in the top left corner of the interior chamber. Install the beveled end of theneoprene stopper in the opening.

f. Installing the HEPA Filter

Remove the filter from the shipping box.

Remove the plastic coating from the filter, using caution not to touch the filter media.

Install the filter as shown in Figure 1-12.

Refer to Section 5.6 for HEPA filter maintenance.

shelf support tab

shelf channelrear slot right side

shelf channel

Figure 1-10

Figure 1-11


1-11

Use caution when handling the filter. The media can be damaged if it ismishandled. To avoid damage to the incubator, do not operate the unit without theHEPA filter in place.

Remove the filter from the shipping box.

Remove the plastic coating from the filter using caution not to touch the filter media.

Install the filter as shown in Figure 1-12.

Refer to Section 5.6 for HEPA filter maintenance.

g. Leveling the Unit

Check the unit for being level by placing a bubble-style level on one of the shelves. Turn the hexnut on the leveler counterclockwise to lengthen the leg, or clockwise to shorten it. Level the unit front-to-back and left-to-right.

Figure 1-12 Blower fan inlet

Temperatureprobe

HEPA filter


1-12

h. Connecting the Unit to Electrical Power

See the serial tag on the side of the unit for electrical specifications, or refer to the electricalschematics in Section 9 of this manual.

Connect the incubator to a grounded dedicated circuit only.

The power cord connector is the mains disconnect device for theincubator. Position the unit so that it can be easily disconnected.

Plug the provided power cord into the power inlet connector (See Figure 1-13) and into thegrounded dedicated circuit.

Electrical Specifications

Models 3110, 3120, 3130, 3140 - 115V 50/60 Hz 3.6A 1 PH 2W

Models 3111, 3121, 3131, 3141 - 230V 50/60 Hz 2.0A 1 PH 2W

.i. Filling the Water Jacket

Turn the power switch on. ADD WATER will appear in the message center. Press the Silencekey to silence the alarm.

Note: The fill port has a plug that must be removed before filling and replaced after filling is complete.

Fill the water jacket with 11.7 gallons (43.5 liters) of water with a quality of 50 K ohm to 1 Megohm. Vinyl tubing and funnel for filling are included in the accessory bag shipped with the unit. Connectvinyl tubing to the fill port. See Figure 1.1.

When the jacket is full, the audible alarm will sound a continuous tone for 10 seconds and the alarmcondition will be cleared. Refer to Section 4.1, Table of Alarms.


1-13

j. Filling the Humidity Pan

Fill the humidity pan with 1.5 gallons (5.68 liters) of sterile, distilled water. Place the pan directly on theincubator floor to ensure best humidity and temperature response.

Check the water level and change it frequently to avoid contamination. Do not allow the water levelto fluctuate significantly. Dry-outs will have an adverse effect on the humidity level.

Figure 1-13, Rear Panel View showing all options


1-14

k. Connecting the CO2 Gas Supply

Warning!High concentrations of CO2 gas can cause asphyxiation

OSHA Standards specify that employee exposure to carbon dioxide in any eight-hour shift of a 40-hour work week shall not exceed the eight-hour time weighted average of 5000 PPM (0.5% CO2).The short term exposure limit for 15 minutes or less is 30,000 PPM (3% CO2). Carbon dioxidemonitors are recommended for confined areas where concentrations of carbon dioxide gas canaccumulate.

The CO2 gas supply being connected should be industrial grade 99.5% pure and should notcontain siphon tubes. Install a two-stage pressure regulator at the cylinder outlet. The high pressuregauge at the tank should have 0-2000 psig range and the low pressure gauge, at the incubator inlet,should have a 0-30 psig range. Input pressure to the incubator must be maintained at 15 psig (103.4kPa).

The incubator has serrated fittings on the back of the cabinet to connect the gas supply. Refer toFigure 1-13. The fitting is labeled CO2 inlet #1 Tank. Make sure that the connections are secured withclamps. Check all fittings for leaks.

For units having the CO2 Gas Guard option, refer to Section 6.2.


1-15

Warning!This incubator is designed to be operated with CO2 gas only. Connecting a flammable or

toxic gas can result in a hazardous condition.

Gases other than CO2 should not be connected to this equipment. CO2 gas cylinders have UN1016

labeled on the cylinder and are equipped with a CGA 320 outlet valve. Check the gas cylinder for the

proper identification labels. The CO2 gas supply being connected to the incubator should be industrial

grade, 99.5% pure. Do not use CO2 gas cylinders equipped with siphon tubes. A siphon tube is used to

extract liquid CO2 from the cylinder, which can damage the pressure regulator. Consult with your gas

supplier to ensure that the CO2 cylinder does not contain a siphon tube. Gas cylinders should also be

secured to a wall or other stationary object to prevent them from tipping.

A two-stage CO2 pressure regulator is required to be installed on the outlet valve of the gas

cylinder. Input pressure to the incubator must be maintained at 15 psig (103.4 kPa) for proper

performance of the CO2 control system. (A single stage CO2 pressure regulator will not maintain 15 psig

(103.4 kPa) to the incubator as the pressure in the CO2 cylinder decreases; therefore, a two stage

regulator is recommended.)

If higher purity CO2 is desires inside the incubator (greater than 99.5% pure), the pressure regulator

should be constructed with a stainless steel diaphragm along with specifying the purity of the CO2 from

the gas supplier. Follow the manufactures instructions to ensure proper and safe installation of the

pressure regulator on the gas cylinder.

Consult your facility safety officer to ensure that the equipment is installed in accordance with the

codes and regulations that are applicable in your area.

l. Connecting the N2 Gas Supply

This connection applies only to those units that have an O2 system. The N2 gas supply beingconnected should be 99.99% pure. Do not use liquid nitrogen. Follow the same steps as in the sectionabove for preparing the N2 tank for hookup to the incubator. For units having the N2 Gas Guard option,refer to Section 6.2. Connect the vinyl lines from the N2 tank to the serrated fitting labeled N2 Inlet andsecure with the provided clamp. Check all fittings for leaks.

1.6 Incubator Start-Up

Now that the incubator has been properly installed, connected to power, filled with water, humiditypan filled, and connected to gas supplies, system setpoints can be entered. The following setpoints can beentered in set mode: temperature, over temperature, CO2, and O2. To enter Set Mode, press the mode


1-16

key until the Set indicator lights. Press the right and/or left arrow keys until the proper parameter appearsin the message center. See Chart 1-1 for more detail.

a. Setting the Operating Temperature

Incubator models 3110, 3111 have an operating temperature range of 10.0 to 55.0 C, models3120, 3121 at 10.0 to 50.0 C, and models 3130, 3131, 3140, and 3141, at 10.0 to 45.0 C. Allunits require the cooling coil option to run at any temperature lower that 5 C above ambient. Theincubator is shipped from the factory with a temperature setpoint of 10.0 C. At this setting all heatersand alarms are turned off. To change the operating temperature setpoint:

Press the Mode Key until the Set indicator lights. Press the right arrow until Temp XX.X is displayed in the message center. Press up/down until the desired temperature setpoint is displayed. Press enter to save the setpoint. Press the Mode Key until the Run Indicator lights to go to run mode or right/left to go to

next/previous parameter.

b. Setting the Overtemp Setpoint

The independent overtemp circuit is designed as a safety to protectthe incubator only. It is not intended to protect or limit the maximumtemperature of the cell cultures or customers equipment inside theincubator if an overtemp condition occurs.

The incubator is equipped with an independent circuit that monitors the air temperature in thecabinet. The independent overtemp circuit is designed as a safety for the incubator only. Should thesystems temperature control fail, this circuit would cut out all heaters when the cabinets temperaturereaches the Overtemp setpoint. When an incubator is operating in an overtemp condition, thetemperature control in the incubator will be 1C around the overtemp setpoint.

The overtemps function is to prevent abnormally high temperatures that will occur if the heatersare locked on as a result of a failure in the main temperature control. Although theovertemp circuit will control the chamber temperature close to the overtemp setpoint, it is not intendedto protect or limit the maximum temperature of the cell cultures or the equipment inside the chamberwhen the overtemp condition occurs.

The factory setting for the Overtemp is 40C. It can be set over a range of temp setpoint +0.5C to 60C. If the temperature setpoint is moved above the Overtemp setpoint, the Overtemp will


1-17

automatically update to 1.0 degrees C + the temp setpoint. It is recommended that the Overtempsetpoint be 1C over the temp setpoint.

To set the Overtemp setpoint:

Press the Mode Key until the Set indicator lights.

Press the right arrow until Otemp XX.X is displayed in the message center.

Press up/down until the desired Overtemp setpoint is displayed.

Press Enter to save the setpoint.

Press the Mode Key until the Run Indicator lights to go to run mode or right/left to go tonext/previous parameter.

c. Setting the CO2 Setpoint

All T/C CO2 cells are precalibrated at the factory at 37C, high humidity, and 10% CO2.Therefore, if a temperature setpoint of 37C has been entered, the humidity pan filled, and the CO2control is to run between 0-10% with a T/C CO2 sensor, the CO2 setpoint may be enteredimmediately. Otherwise, it is important to allow the unit 12 hours to stabilize at the temperature setpointbefore entering the CO2 setpoint.

All models of the incubator have a CO2 setpoint range of 0.0% to 20.0%. The incubator isshipped from the factory with a CO2 setpoint of 0.0%. At this setting, all CO2 control and alarms areturned off. To change the CO2 setpoint:


Press the right arrow until CO2 XX.X is displayed in the message center.

Press up/down until the desired CO2 setpoint is displayed.



d. Setting the O2 Setpoint

Models 3130, 3131, 3140, and 3141 of the incubator have a built-in O2 control system. TheO2 setpoint range is 2.0% to 21.0%. The incubator is shipped from the factory with a O2 setpoint of21.0%. At this setting, all O2 control and alarms are turned off. To change the O2 setpoint:


Press the right arrow until O2 XX.X is displayed in the message center.

Press up/down until the desired O2 setpoint is displayed.



1-18



1-20

Forma Model 3100/3200 Series _________________________________________Calibration

2-1

Section 2 - Calibration

2.1 Calibration Mode

After the unit has stabilized, several different systems can be calibrated. In the CalibrationMode the air temperature, CO2 reading, O2 reading, and RH reading can all be calibrated toreference instruments. To enter Calibrate Mode, press the mode key unit the CAL indicatorlights. Press the right and/or left arrow until the proper parameter appears in the message center.See Chart 3-1 for more detail.

Calibration frequency is dependent on use, ambient conditions and accuracy required. Goodlaboratory practice would require at least an annual calibration check. On new installations, allparameters should be checked after stabilization periods.

Prior to calibration, the user should be aware of the following system functions. While theunit is in the calibration mode, all system control functions will be stopped so that the unitremains stable. Readout of the system being calibrated will appear as --- on the readoutdisplays. If no keys are pressed for approximately five minutes while in Calibrate Mode, thesystem will reset to Run Mode so that control functions can be reactivated.

Before making an calibration or adjustments to the unit, it isimperative that all reference instruments be properlycalibrated.

a. Calibrating the Temperature

Place the calibrated instrument in the center of the chamber. The instrument should be inthe air flow, not against the shelf. Before calibration, allow the cabinet temperature to stabilize.

Temperature Stabilization Periods

Start-Up - Allow 12 hours for the temperature in the cabinet to stabilize before proceeding. Operating Unit -Allow at least two hours after the display reaches setpoint for the temperature

to stabilize before proceeding.


2-2

Press the Mode key until the CAL indicator lights. Press the right arrow until TEMPCAL XX.X appears in the message center. Press the up/down arrow to match the display to a calibrated instrument. Press Enter to store the calibration into memory. Press the Mode key to return to Run. OR the right/left arrow to go to the

next/previous parameter. b. Calibrating the Thermal Conductivity CO2 System

Models 3110, 3111, 3130 and 3131 have a thermal conductivity (T/C) CO2 sensor. Thermalconductivity of the incubator atmosphere is not only effected by the quantity of CO2 present, but alsoby the O2%, the air temperature, and the quantity of water vapor present in the incubator atmosphere.In monitoring the effects of CO2, O2%, air temperature, and absolute humidity must be held constantso any change in thermal conductivity is caused only by a change in CO2 concentration.

Changing temperature or O2% set points or changing from elevated humidity levels to roomambient humidity levels would necessitate a recalibration of the CO2 control. Models fitted with the O2control option, (models 3130 and 3131) have an O2 compensation feature. Refer to the instructions inSection 3.1.s of this manual, for enabling O2 compensation for T/C Systems. When this feature isenabled, the processor will automatically adjust the T/C CO2 sensor for changes in the O2 percentage.

New Installation. The CO2 sensor has been calibrated at the factory for 37C and elevatedhumidity. Allow the incubator to run at least 12 hours before checking CO2 concentration with anindependent instrument. If the incubator is operating at 37C, a zero adjustment is all that is necessary.

Press the Mode key until CAL indicator lights. Press the right arrow until CO2ZER0 XX.X is displayed in the message center. Press up/down arrow to change the display to match the measuring device. Press Enter to store the calibration. Press the Mode key to return to run or right/left arrow to go to next/previous parameter.

If the temperature setpoint is other than 37, a zero and span adjustment is necessary.Never adjust span without first ensuring the zero is adjusted to ambient CO2, which isapproximately 0% CO2.


2-3

c. Calibrating the T/C %CO2 Zero to Ambient

On models with O2 control (models 3130 and 3131) you must disable the O2compensation before performing a complete CO2 calibration. Check Section 3.1 ConfigurationMode, Enabling O2 Compensation for T/C CO2 Systems. After completion of the CO2calibration, you may return to the Configuration Mode and enable the O2 compensation feature.

T/C CO2 Sensor Stabilization PeriodsStart Up- Set CO2 set point to 0% (which disables CO2 valve) or shut CO2 off at the supplytank. Allow temperature, humidity, and O2 levels in the chamber to stabilize at least 12hours before proceeding.

Operating Unit- Set CO2 set point to 0% (which disables CO2 valve) or shut off CO2 at thesupply tank. Open both doors and air-out chamber for at least 2 minutes. Close doors andallow temperature, humidity and O2 levels to stabilize for at least 2 hours before proceeding.

Ensure stabilization periods as outlined above are followed. Press the Mode key until the CAL indicator lights. Press the right arrow until CO2 ZERO XX.X is displayed in the message center. Press up/down arrow to adjust display to 0.0%. Press Enter to store calibration. Press the Mode key to return to run mode.

Adjust the CO2 setpoint to the desired level. Turn on the CO2 at the supply tank. Allow CO2 tostabilize at least 15 minutes after CO2 actual display reaches setpoint. Measure the chamber CO2content through the gas sample port with a Fyrite or other independent instrument. Several readingsshould be taken to ensure accuracy. If the display does not match the independent instrumentreading, proceed to the CO2 span adjustment.

d. Calibrating the T/C %CO2 Span

To insure accurate calibration, the unit will not allow CO2 to be spanned below 3%. Ifthe cabinet does not contain at least 3.0% CO2, increase the setpoint and allow the unit tostabilize before completing this procedure.

Ensure CO2 zero adjustment has been properly followed before continuing. Press the Mode key until the CAL indicator lights. Press the right arrow until CO2SPAN XX.X appears in the message center. Press the up/down arrow to adjust the display to match the independent instrument reading. Press Enter to store the calibration. Press the Mode key to return to run mode.


2-4

e. Calibrating the Infra-Red CO2 System.

Models 3120, 3121, 3140 and 3141 have an infra-red CO2 sensor. Infra-red CO2 sensors arenot effected by chamber atmosphere temperature, humidity and O2 variations. However, the lightdetector in the sensor is effected by wide temperature changes. Therefore, changing temperaturesetpoints could necessitate a recalibration of the CO2. Chamber temperature should be allowed tostabilize before checking CO2 concentrations with an independent instrument, especially on start-up.

All models equipped with an IR/CO2 sensor have an automatic calibration which occursevery 24 hours, and lasts for 5 to 6 minutes. During automatic calibration, the CO2 display isblanked out and HEPA filtered room air is pumped through the CO2 sensor. A new CO2 calibrationvalue is stored in memory for use as the 0.0% CO2 reference point. The keypad/ control panel islocked up during calibration, with the message center reading CO2 AUTO CAL.

IR CO2 Sensor Stabilization Times

Startup- Allow the temperature and the CO2 of the cabinet to stabilize at least 12 hoursbefore proceeding.

Operating Unit - Allow CO2 to stabilize at least 2 hours at set point before proceeding.

To insure accurate calibration, the unit will not allow CO2 to be spanned below3%. If the cabinet does not contain at least 3.0% CO2, increase the setpoint andallow the unit to stabilize before completing this procedure.

Measure the CO2 concentration in the chamber through the gas sample port with a Fyrite orother independent instrument. Several readings should be taken to ensure accuracy.

Press the Mode key until the CAL indicator lights. Press the right arrow until IR CAL XX.X appears in the message center. Press the up/down arrow to adjust the display to match the independent instrument reading. Press Enter to store calibration. Once Enter is pressed, the unit will go into a calibration cycle that will last 5 to 6 minutes.

The control panel is locked up during this calibration cycle. Press the Mode key to return to run mode.


2-5

f. Calibrating the O2 system.

If using an O2 Fyrite, the accuracy of the instrument will be greatly affected bythe concentration of CO2 in the cabinet. Refer to the Fyrite operating manual.

Models 3130, 3131, 3140 and 3141 have an O2 control sensor. The sensor is a fuel cell whichputs out a linear millivolt signal based on O2 content of the chamber. The fuel cell depletes over timedepending on O2 levels. The above models have an automatic calibration to maintain the O2calibration as the fuel cell depletes. The automatic calibration occurs every 12 hours and lasts 1 to 3minutes. During automatic calibration, the O2 display is locked on the existing O2 reading andHEPA filtered room air is pumped through the O2 sensor.

A new O2 span value is stored in memory for use as the 20.7% O2 reference point. Thekeypad/control panel is locked-up during automatic calibration.

O2 Sensor Stabilization TimesStartup - Allow the cabinet to stabilize at least 12 hours before proceeding.

Operating Unit - Allow O2 to stabilize at least 2 hours at set point before proceeding.

Measure the O2 concentration in the chamber through the gas sample port with anindependent instrument. Several readings should be taken to ensure accuracy.

Press the Mode key until the CAL indicator lights. Press the right arrow until O2 CAL XX.X appears in the message center. Press up/down adjust display to independent instrument reading. Press Enter to store calibration. Press the Mode key to return to run mode.

g. Calibrating Relative Humidity

All 3110 Series incubators can be equipped with an optional direct readout relativehumidity sensor. This is a readout only of the chamber relative humidity. It does not provide anycontrol of the relative humidity in the cabinet.

Relative Humidity Stabilization TimesStartup - Allow 12 hours for the relative humidity and temperature in the chamber tostabilize before proceeding.

Operating Unit - Allow at least 2 hours after temperature display reaches set point forrelative humidity to stabilize before proceeding.


2-6

Place an accurate independent instrument in the center of the chamber. Allow at least30 minutes for RH to stabilize.

Press the Mode key until the CAL indicator lights. Press the right arrow key until RH CAL XX appears in the message center. Press the up/down arrow to match the display to the independent instrument. Press Enter to store the calibration. Press the mode key to return to run mode.If a reliable RH measuring device is not available, you may calibrate the display to a

typical level. On units with O2 control, the nitrogen must be shut off as the incoming nitrogenlowers the optimum achievable level of RH in the chamber. Follow the RH stabilization periodsoutlined above. With a full humidity pan and stable temperature, the relative humidity in thechamber will be 95%. In step 5 of the relative humidity sensor adjustment, you may adjust thedisplay to 95%. This calibration method should be accurate to within 5%.


2-7

Silence Mode

Mode

Mode

Mode

Mode

Mode

CALIBRATE

TEMP XX.X C

CO2 SP XX.X %

IR CO2 XX.X %

O2 XX.X%

RH XX %

36.9

4.9Enter

Enter

Enter

Enter

Enter

Enter

Numbers increase

Numbers increase

Numbers increase

Numbers increase

Numbers increase

Numbers increase

Press MODE to move to SYS CONFIG mode

Numbers decrease

Numbers decrease

Numbers decrease

Numbers decrease

Numbers decrease

Numbers decrease

Press Enter tosave the setting

Run Set Cal Config Heat Temp

Inject % CO2Scroll for Parameters

Scroll for Parameters





Mode CO2 ZR XX.X % Enter

Press to return to previous parameter




Percent O

Percent RH

Span CO

Zero CO

OperatingTemperature

Press MODE to light CAL

To Calibrate:

Span CO2

(when O option is in use)

(when IR sensor is in use)

(when Thermal Conductivitysensor is in use)

(when Thermal Conductivitysensor is in use)

(when RH option is in use)

2

2

2

2

Calibrate Mode

Chart 2-1

Modes-a4.cdr

Forma Model 3100/3200 Series _________________________________________Configuration

3-1

Section 3 - Configuration

3.1 Configuration Mode

There are many features available in Configuration mode that allow custom setup of the incubator.These features are listed and described below. All features may not be necessary in all applications, but areavailable if needed. To enter Configuration mode, press the Mode key until the Config indicator lights.Press the right or left arrow until the appropriate parameter appears in the message center. See Chart 3-1for more detail.

a. Turning the Audible Alarm ON/OFF

The audible alarm can be turned on or off. The factory setting is ON.

Press the Mode key until the Config indicator lights. Press the right arrow until Audible XXX is displayed in the message center. Press up/down to toggle Audible ON/OFF. Press Enter to save the setting. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

b. Setting an Access Code

A three-digit Access Code can be entered to avoid unauthorized personnel from changing thesetpoints, calibration, or configuration. A setting of 000 will bypass the access code. The factory setting is000.

Press the Mode key until the Config indicator lights. Press the right arrow until Acc Code XXX is displayed in the message center. Press up/down to change the access code. Press Enter to save the access code. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

c. Setting a Low Temp Alarm Limit (tracking alarm)

The low temp alarm limit is the deviation from the temperature setpoint which will cause a low tempalarm. The low temp alarm is variable from 0.5 below setpoint to 5.0 below setpoint. The factory settingis 1.0 below setpoint. A minus sign (-) in the display indicates that the alarm setting is below the setpoint.

Press the Mode key until the Config indicator lights. Press the right arrow until Temp Lo -X.X is displayed in the message center. Press up/down to change the low temp alarm limit. Press Enter to save the low temp alarm limit. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.


3-2

d. Setting a High Temp Alarm Limit (tracking alarm)

The high temp alarm limit is the deviation from the temperature setpoint which will cause a high temp alarm. Itshould be noted that this varies from the Overtemp setpoint, in that the Overtemp setpoint configures anindependent system that monitors temperature and shuts down the system heaters if necessary. The high tempalarm limit is simply set to enable an audible and visual alarm which notifies the user of a problem. The high tempalarm is variable from 0.5 above setpoint to 5.0 above setpoint. The factory setting is 1.0 above setpoint.

Press the Mode key until the Config indicator lights. Press the right arrow until Temp Hi X.X is displayed in the message center. Press up/down to change the high temp alarm limit. Press Enter to save the high temp alarm limit. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

e. Enabling Temperature Alarms to Trip Contacts

High and Low temperature alarms can be programmed to trip the remote alarm contacts. A setting of ONwill cause this, a setting of OFF will not allow temp alarms to trip the contacts. The factory setting is ON.

Press the Mode key until the Config indicator lights. Press the right arrow until Tmp Rly XXX is displayed in the message center. Press up/down to toggle the setting ON/OFF. Press Enter to save the setting Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

f. Setting a Low CO2 Alarm Limit (tracking alarm)

The low CO2 alarm limit is the deviation from the CO2 setpoint which will cause a low CO2 alarm. Thesetpoint is variable from 0.5% CO2 below setpoint to 5.0% CO2 below setpoint. The factory setting is 1.0%CO2 below setpoint. A minus (-) in the display indicates that the alarm setting is below the setpoint.

Press the Mode Key until the Config indicator lights. Press the right arrow until CO2 Lo -X.X is displayed in the message center. Press up/down to change the low CO2 alarm limit. Press Enter to save the low CO2 alarm limit. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.


3-3

g. Setting a High CO2 Alarm Limit (tracking alarm)

The high CO2 alarm limit is the deviation from the CO2 setpoint which will cause a high CO2 alarm.The setpoint is variable from 0.5% CO2 above setpoint to 5.0% CO2 above setpoint. The factory setting is1.0% CO2 above setpoint.

Press the Mode key until the Config indicator lights. Press the right arrow until CO2 Hi X.X is displayed in the message center. Press up/down to change the high CO2 alarm limit. Press Enter to save the high CO2 alarm limit. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

h. Enabling CO2 Alarms to Trip Contacts

High and Low CO2 alarms can be programmed to trip the remote alarm contacts. A setting of ON willcause this; a setting of OFF will not allow CO2 alarms to trip the contacts. The factory setting is ON.

Press the Mode key until the Config indicator lights. Press the right arrow until CO2 Rly XXX is displayed in the message center. Press up/down to toggle the setting ON/OFF. Press Enter to save the setting. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

i. Setting a New Zero Number for T/C CO2 Sensors

If a new T/C CO2 sensor is being installed, the two numbers on the factory installed sticker on the T/C cellmust be entered to calibrate the CO2 in the unit.

NOTE: For the technicians convenience, a label containing the two numbers on the T/C cell is affixed inside the electronics drawer.

Press the Mode key until the Config indicator lights. Press the right arrow until T/CZ# XXXX is displayed in the message center. Press up/down to change the zero number to match the sticker. Press Enter to save the setting. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.


3-4

j. Setting a New Span Number for T/C CO2 Sensors

If a new T/C CO2 sensor is being installed, the two numbers on the factory installed sticker on the T/C cellmust be entered to calibrate the CO2 in the unit.

NOTE: For the technicians convenience, a label containing the two numbers on the T/C cell is affixedinside the electronics drawer.

Press the Mode key until the Config indicator lights. Press the right arrow until T/CS# XXXX is displayed in the message center. Press up/down to change the span number to match the sticker. Press Enter to save the setting. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

k. Setting a Low RH Alarm Limit

On units that have the RH option installed, a low RH alarm limit may be entered. The low RH alarmlimit is the %RH in the cabinet that will cause a low RH alarm. The setpoint is variable from setpoint 0 to 90%RH. The factory setting is 0% RH, which will disable the alarm.

Press the Mode key until the Config indicator lights. Press the right arrow until RH Lo XX is displayed in the message center. Press up/down to change the RH low alarm limit. Press Enter to save the RH low alarm limit. Press the Mode key to return to run mode or right/left to go to next/previous parameter.

l. Enabling RH Alarms to Trip Contacts

The low RH alarm can be programmed to trip the remote alarm contacts. A setting of ON will cause this, asetting of OFF will not allow the RH alarm to trip the contacts. The factory setting is ON.

Press the Mode key until the Config indicator lights. Press the right arrow until RH Rly XXX is displayed in the message center. Press up/down to toggle the setting ON/OFF. Press Enter to save the setting. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.


3-5

m. New O2 Cell

On models equipped with the O2 control option, the units microprocessor monitors the life of the O2sensor. When the O2 sensor needs replaced, an alarm will sound and the message REPL O2 SNSR will bedisplayed. Once a new O2 sensor is installed, this menu will allow it to be automatically calibrated. To performthe automatic calibration:

Press the Mode key until the Config indicator lights. Press the right arrow until NEW O2 CELL is displayed in the message center. Press Enter to start calibration. Calibration will take 3-5 minutes. Once completed, press the Mode key to return to Run mode.

n. Setting a Low O2 Alarm Limit (tracking alarm)

On models with a O2 control system, O2 alarms may be configured. The low O2 alarm limit is the deviationfrom the O2 setpoint that will cause a low O2 alarm. The setpoint is variable from 0.5% O2 below setpoint to5.0% O2 below setpoint. The factory setting is 1.0% O2 below setpoint. A minus (-) in the display indicatesthat the alarm setting is below setpoint.

Press the Mode key until the Config indicator lights. Press the right arrow until O2 Lo -X.X is displayed in the message center. Press up/down to change the low O2 alarm limit. Press Enter to save the low O2 alarm limit. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

o. Setting a High O2 Alarm Limit (tracking alarm)

On models with a O2 control system, O2 alarms may be configured. The high O2 alarm limit is the deviationfrom the O2 setpoint which will cause a high O2 alarm. The setpoint is variable from 0.5% O2 above setpoint to5.0% O2 above setpoint. The factory setting is 1.0% O2 above setpoint.

Press the Mode key until the Config indicator lights. Press the right arrow until O2 Hi X.X is displayed in the message center. Press up/down to change the high O2 alarm limit. Press Enter to save the high O2 alarm limit. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.


3-6

p. Enabling O2 Alarms to Trip Contacts

On models with an O2 control system, O2 alarm contacts may be configured to trip the contacts. Asetting of ON will cause this, a setting of OFF will not allow O2 alarms to trip the contacts. The factorysetting is ON.

Press the Mode key until the Config indicator lights. Press the right arrow until O2 Rly XXX is displayed in the message center. Press up/down to toggle the setting ON/OFF. Press Enter to save the setting. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

q. Enabling Temp/RH to be Displayed

On units that are equipped with the RH option, the upper seven-segment display on the control panel canbe configured to display Temp continuously, RH continuously, or toggle between Temp and RH. If the unitsdoes not have RH, the upper display will always display temperature. If temperature is set to ON and the RH isset OFF, temperature will be displayed continuously. If temperature is set to OFF and RH is set to ON, RH willbe displayed continuously. If both are turned ON, the display will toggle between the two. The factory settingwill default to toggle mode if the RH option is present.

Press the Mode key until the Config indicator lights. Press the right arrow until Disp Tmp XXX or Disp RH XXX is displayed in the message center. Press up/down to toggle the setting ON/OFF. Press Enter to save the setting. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

r. Enabling CO2/O2 to be Displayed

On models that are equipped with the O2 system, the upper seven-segment display on the control panelcan be configured to display CO2 continuously, O2 continuously, or toggle between CO2 and O2. If the unitsdoes not have O2, the upper display will always display CO2. If CO2 is set to ON and the O2 is set OFF,CO2 will be displayed continuously. If CO2 is set to OFF and O2 is set to ON, O2 will be displayedcontinuously. If both are turned ON, the display will toggle between the two. The factory setting will default totoggle mode if the O2 system is present.

Press the Mode key until the Config indicator lights. Press the right arrow until Disp CO2 XXX or Disp O2 XXX is displayed in the message center. Press up/down to toggle the setpoint. Press Enter to save the setpoint. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.


3-7

s. Enabling O2 Compensation for T/C CO2 Systems

On models that have T/C CO2 control and a O2 control system, the T/C CO2 reading is susceptible toO2 levels in the chamber. The incubator is capable of compensating the CO2 display for the varying O2level. A setting of ON will allow such compensation. The factory setting is OFF.

Press the Mode key until the Config indicator lights. Press the right arrow until O2 Comp XXX is displayed in the message center. Press up/down to toggle the setting ON/OFF. Press Enter to save the setting. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

t. Setting a RS485 Communications Address (1535 compatible only)

On units that have the RS485 option, direct communication with the Forma 1535 Model alarm systemcan be established. Each piece of equipment connected to the 1535 must have a unique address. Anaddress of 0-24 can be entered for the incubator. A setting of 0 is an invalid address that the 1535 willignore. The factory setting for the RS485 address is 0.

Press the Mode key until the Config indicator lights. Press the right arrow until RS485 XX is displayed in the message center. Press up/down to move the RS485 address. Press Enter to save the RS485 address. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

u. Selecting a Primary Tank with the Gas Guard Option

On units equipped with the Gas Guard option, a primary tank can be selected. The primary tank will beeither Tank 1 or Tank 2. The factory setting is Tank 1.

Press the Mode key until the Config indicator lights. Press the right arrow until Tnk Sel X is displayed in the message center. Press up/down to toggle the setting between 1 and 2 . Press Enter to save the setting. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

v. Enabling the Gas Guard System

On units equipped with the Gas Guard option, the Gas Guard system may be turned ON or OFF if it isnot in use. The factory setting is OFF.

Press the Mode key until the Config indicator lights. Press the right arrow until Gas Grd XX is displayed in the message center. Press up/down to toggle the setting ON/OFF. Press Enter to save the setting.


3-8

Press the Mode key to return to Run mode or right/left to go to next/previous parameter.

w. Setting the Door Heat

The incubator is equipped with a built-in heater on the inner glass door. The percentage of time thatpower is applied to this heater as compared to the main heater can be varied from 0 to 99%. The factorysetting is 50%. Changing this setting can affect the units temperature control and uniformity. Consult thefactory before changing this setting.

Press the Mode key until the Config indicator lights. Press the right arrow until Dr Heat XX is displayed in the message center. Press up/down to move the door heat setpoint. Press Enter to save the door heat setpoint. Press the Mode key to return to Run mode or right/left to go to next/previous parameter.


3-9


3-10

Mode

Mode

Mode

Mode

Mode

Mode

Mode

CO HI X.X %2

CO2 RLY XXX

T/C ZR# -XXXX

IR Z&S XX.X

RH RLY XXX

NEW O2 CELL

T/C SP# +XXXX

RH LO X%

Enter

Enter

Enter

Enter

Enter

Enter

Enter

Numbers increase

Enable relay contacts (ON)during RH alarm

Numbers increase

Numbers increase

Numbers decrease

Disable relay contacts (OFF)during RH alarm

Numbers decrease

Numbers decrease

Start Auto Hardware Calib.

Press Enter to

save the setting

Numbers increase

Numbers decrease

Press Enter to

save the setting

Enable relay contacts (ON)

during CO2 alarms

Disable relay contacts (OFF)during CO2 alarms

Press Enter to

save the setting


Press Enter to

save the setting

Press Enter to

save the setting

Numbers increase

Numbers decrease

3-1p2.cdr

Press Enter tostart calibration

S c r o l l f o r P a r a m e t e r s







Mode Enter



Press to return

to previous parameter


Press to return


Press to return


Press to return




refer to next page

refer to previous page

Configure Mode, Chart 3-1, Page 2 of 4

T/C Zero Number

IR Sensor Hardware

Calibration

RH Relay ON/OFF

New O2 Cell

Calibration

CO % High Limit2

CO Relay ON/OFF2

To Configure:

T/C Span Number

RH Low Alarm Limit


3-11

Mode

Mode

Mode

Mode

Mode

Mode

Mode

O2 RLY XXX

O2 HI XX.X%

O2 LO X.X%

DISP TEMP X XX

DISP RH XXX

O2 COMP XXX

DISP CO2 XXX

DISP O2 XXX

Enter

Enter

Enter

Enter

Enter

Enter

Enter

Display RH (ON)

Enable relay contacts (ON)during O alarms2

Compensation ON

Display CO (ON)2

Display O (ON)2

Numbers increase

Numbers increase

Numbers decrease

Numbers decrease

Display RH (OFF)

Disable relay contacts (OFF)during O alarms2

Compensation OFF

Dispaly CO (OFF)2

Display O (OFF)2

Press Enter to

save the setting

Press Enter to

save the setting

Press Enter to

save the setting

Display temp continuously (ON)

Display temp continuously (OFF)

Press Enter to

save the setting

Press Enter to

save the setting


Press Enter to

save the setting

3-1p3.cdr

Press Enter to

save the setting








Mode Enter


Press to return


Press to return








refer to next page



Display RH ON/OFF

Display O ON/OFF2

O Relay ON/OFF2

O High Limit2

O Low Limit2

Display Temperature

To Configure:

Display CO ON/OFF2

O Compensation

ON/OFF2


3-12



To Configure:

Mode

Mode

Enter

Enter

Scro l l fo r Parameters




GAS GRD XXX

TANK SEL X

RS 485 XX

Gas Guard ON/OFF

Tank Select

RS485

Mode Enter

Numbers increase

Numbers decrease




Mode Enter

Numbers increase

Numbers decrease




DR HEAT XX%Percent Door Heat

Tank 1 prime

Numbers increase

Numbers decrease

Tank 2 prime

Press Enter to save the setting

Press Enter to save the setting

3-1p4.cdr

Forma Model 3100/3200 Series ___________________________________________ Alarms

4-1

Section 4 - Alarms

4.1 Alarms

The Model 3110 Series incubator alarm system is shown in the table below. When an alarm isactive, the message appears in the LED message center. Pressing Silence disables the audible alarm forthe ringback period. However, the visual alarm continues until the incubator returns to a normalcondition. The alarms are momentary alarms only. When an alarm condition occurs and then returns tonormal, the incubator automatically clears the alarm condition and the message center.

Description Message Delay Ringback RelayNo alarm condition exists SYSTEM OK ----- ----- -----CO2 System Auto Calibrating CO2 AUTO CAL ----- ----- -----Temp > Otemp Set point SYS IN OTEMP 0 min. 15 min. YesWater Temp Sensor Fault (See Sect 4.2) H2O SENS ERR 0 min. 15 min. NoAir Temp Sensor Fault (See Sect 4.2) AIR SENS ERR 0 min. 15 min. NoCO2 Sensor Fault (See Sect 4.5) CO2 SENS ERR 0 min. 15 min. NoO2 Sensor Fault(O2 option only) (See Sect 4.2)

O2 SENS ERR 0 min. 15 min. No

O2 Sensor Low(O2 option only) (See Sect 4.3)

REPL O2 SNSR 15 min. 15 min. No

0CO2 Sensor cannot be calibrated (IR option only) (See Sect. 4.4)

REPL IR SENSR 0 min. 15 min. No

Water low in jacket ADD WATER 0 min. 15 min. NoInner Door is Open DOOR OPEN 15 min. 15 min. NoCO2 > CO2 High Tracking Alarm CO2 IS HIGH 15 min. 15 min. ProgrammableCO2 < CO2 Low Tracking Alarm CO2 IS LOW 15 min. 15 min.TEMP > TEMP High Tracking Alarm TEMP IS HIGH 0 min. 15 min. ProgrammableTEMP < TEMP Low Tracking Alarm TEMP IS LOW 15 min. 15 min.O2 > O2 High Tracking Alarm (O2 option only) O2 IS HIGH 15 min. 15 min. ProgrammableO2 < O2 Low Tracking Alarm (O2 option only) O2 IS LOW 15 min. 15 min.RH < RH Low Limit Alarm (RH option only) RH IS LOW 30 min. 30 min. ProgrammableO2 Auto-Zero Fault (See Sect 4.2) O2 AUTOZ ERR 0 min. 15 min. NoCO2 Auto-Zero Fault(IR option only) (See Sect 4.6)

IR AUTOZ ERR 0 min. 15 min. No

Tank 1 is low, switch to Tank 2 (Gas Guardonly)

TANK1 LOW 0 min. ---- No

Tank 2 is low, switch to Tank 1 (Gas Guardonly)

TANK2 LOW 0 min. ---- No

Both tanks are low (Gas Guard only) TANK 1 and 2 LOW 0 min. 15 min. No

- All alarm delays and ringback times are +/- 30 seconds -


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When multiple alarm conditions occur, active messages are displayed in the message centerone at a time, updating at 5 second intervals. Pressing Silence during multiple alarms causes all activealarms to be silenced and to ringback in 15 minutes.

The temperature alarms are disabled when the Temp set point is 10C. The CO2 alarms aredisabled when the CO2 set point is 0.0%. The O2 alarms are disabled when the O2 set point is 21.0%.

4.2 Sensor Fault Alarms

The microprocessor in all 3110 series incubators continually scans all available sensorsincluding the pump on O2 units to ensure that they are operating properly. Should an error bedetected, the incubator will sound an alarm and display the appropriate message. If such an alarmoccurs, contact your local distributor or the Forma Scientific service department at 740-373-4763or 1-888-213-1790 (USA and Canada) or fax 740-373-4189.

4.3 REPL O2 SNSR (Alarm)

On units equipped with the O2 system, the microprocessor continually checks the remaininglife of the O2 sensor. If the O2 sensor functionality is declining, the REPL O2 SNSR alarm willsound. This alarm alerts the user of the need to replace the O2 sensor at the earliest convenience. Theunit will continue to function for some length of time. To order a new sensor, contact your localdistributor or the Forma Scientific service department at 740-373-4763 or 1-888-213-1790 (USAand Canada) or fax 740-373-4189.

After the O2 sensor is replaced, see Section New O2 Cell, Section 3.1.m on page 3-5 of thismanual for calibrating the O2 system.

4.4 REPL IR SNSR (Alarm)

On units equipped with an IR CO2 control system, calibration is done automatically using anAuto Zero system. If this system cannot properly calibrate the sensor, the REPL IR SNSR alarmwill sound. The unit will continue to function for some length of time. To order a new sensor,contact your local distributor or the Forma Scientific service department at 740-373-4763 or 1-888-213-1790 (USA and Canada) or fax 740-373-4189.

4.5 CO2 SENS ERR

If the cables or connectors between the main microprocessor board and the CO2 sensor, orbetween the CO2 board and the sensor head on I/R CO2 units become loose or disconnected, theCO2 SENS ERR alarm will occur.


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I/R UnitsOn I/R incubators, the red light on the I/R module (Refer to Figure 5-4 for the location of the

module circuit board) will be lit continuously if communication is lost between the CO2 board andthe sensor head. The CO2 display will also be locked at 00.0 without injection. Turning theincubator off and on will not clear the alarm. Only proper connections of all the components willcorrect the alarm.

4.6 IR AUTOZ ERR

On incubators equipped with I/R CO2 control, calibration is done automatically using anAuto Zero system. Auto Zero occurs once every 24 hours. If, during the Auto Zero cycle, a CO2correction of more than 0.45% is detected, the IR AUTOZ ERR alarm will occur.

Possible problems which will cause this alarm are:

Auto Zero pump, orifice, filter or tubing will not let air to the sensor. Possibilities are: Defective or electrically disconnected air pump

Kinked auto zero vinyl tubing

Disconnected tubing between the air pump and the sensor

Plugged filter or orifice on the auto zero assembly

Defective auto zero circuit

Cabinet temperature has been increased significantly from a previous set point. (For example,the unit was calibrated and operating at 30C and the setpoint is increased to 50C) In thisinstance, calibrating the CO2 will correct the alarm.

There is high background CO2 in the laboratory. This could be from leaks in the tank, regulatoror vinyl CO2 tubing.

High CO2 sensor calibration drift occurred. This will require replacement of the sensor.

Forma Model 3110/3200 Series _____________________________________Maintenance

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Section 5: Routine Maintenance

Before using any cleaning or decontamination method except thoserecommended by the manufacturer, users must check with themanufacturer that the proposed method will not damage theequipment.

5.1 Disinfecting the Incubator Interior

If the units have been in service, disconnect the power cord connectorbefore disinfecting.

Use an appropriate disinfectant. All articles and surfaces to be disinfected must be thoroughlycleaned, rinsed and roughdried.

Alcohol, even a 70% solution, is volatile and flammable Use it only in awell ventilated area that is free from open flame. If any component iscleaned with alcohol, do not expose the component to open flame orother possible hazard. Allow the alcohol to fully dry before turningpower on.

Do not spray the T/C sensor with flammable solutions. The internaltemperature of the CO2 sensor is approximately 150C when the unit isin operation. Allow sufficient time for the sensor to cool beforecleaning.

Do not use strong alkaline or caustic agents. Stainless steel iscorrosion resistant, not corrosion proof.

Do not use solutions of sodium hypochlorite ( Purex, Clorox, etc.), asthey may also cause pitting and rusting.

1. Turn off the incubator power switch and unplug the power cord.

2. Remove the shelves, HEPA filter, access port stopper, left and right duct sheets, and the top duct.The top duct requires the two wing nuts to be removed.


5-2

3. Set the HEPA filter aside. Wash the shelves, ducts, wing nuts and stopper with disinfectant andrinse with sterile water. Optional: The shelves, ducts and wing nuts may be autoclaved.

4. Remove the blower scroll held on by four screws. Remove the blower wheel and the sample portfilter. If a new blower wheel and scroll are going to be used, discard the old ones. If the old onesare being reused, wash all parts with disinfectant and rinse.

5. The blower plate may be lowered by removing five wing nuts and one hex nut. This will allow theblower gasket to be removed and cleaned or replaced. The CO2 sensor may be lowered byremoving two wing nuts. This will allow the CO2 sensor gasket to be removed and cleaned, orreplaced.

6. Wash the inner door gasket with disinfectant and rinse. The inner door gasket may be removed andautoclaved, or replaced.

7. Wash the cabinet interior with disinfectant starting at the top and working down. Wash the innerdoor both inside and out. The cabinet and door must be rinsed with sterile water until thedisinfectant has been removed. After the cabinet has been rinsed, spray with 70% alcohol.

8. If the CO2 sensor gasket and motor plate gasket were removed, spray with 70% alcohol and installthem. Install the sample filter.

9. Install the top duct, left and right ducts, inner door gasket, and access port stopper, spraying eachwith 70% alcohol.

10. Install the HEPA Filter

11. Install the shelves and spray with 70% alcohol.

5.2 Cleaning the Cabinet Exterior

Clean the incubator exterior with a damp sponge or soft, well-wrung cloth and mild detergentdissolved in water. Dry with a soft cloth.

5.3 Cleaning the Humidity Pan

Clean the humidity pan with soap and water and a general use laboratory disinfectant. Rinsewith sterile water and spray with 70% alcohol. The humidity pan may be autoclaved.


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5.4 Reversing the Door Swing

For side-by-side operation or changing lab layouts, the inner and outer doors are fieldreversible. The procedure is written from the prospective of changing the door swing from aleft-hand to a right-hand swing. All screw holes are pre-drilled for reversing the door. The toolsrequired are a Phillips screwdriver and a flat blade screwdriver.

If the units have been in service, disconnect the powercord connector before reversing the door swing.

a. Reversing the Hinges for the Exterior Door

Review Illustration A on Page 5-6. The following instructions will refer to the letters within the circles.

1. Open the outer door and remove it by lifting it off its hinge pins. Lay this door down on its face on apadded surface to prevent scratches.

2. Remove the two outer door hinges identified by the A in the illustration.

3. Remove the four nylon screws in locations B, which will be the new locations for the inner doorhinges.

4. With the Phillips screwdriver, remove and save the four nylon screws from the outer door hingemounting holes, noted as locations C on the right side of the door frame.

5. Remove the electrical connector on the top of theinner door hinge by carefully prying up the blackstrain relief. Refer to item D in the illustration andthe drawing in Figure 5-1. The heater wiringconnector is of yellow rubber which should be visiblewhen the strain relief is moved upward as shown.Refer to the connector manufacturersinstructions in Appendix A of this manual.

6. Grasp the upper portion of the yellow connector andpull it up and out of the hinge socket.

Figure 5-1


5-4

7. Remove the inner door upper hinge, shown as E. This hinge will be inverted and become thelower inner door hinge when the hardware is reassembled. When removing the door, set it aside ona flat surface, taking care not to damage the electrical sockets on the top and bottom of the doorframe. At this time, remove the 5/8 black plastic bushing from the hinge. This bushing will beinserted in the top hinge when the door is installed on the right side of the cabinet.

The frame along the hinge side of the inner glass door has electricalconnectors mounted on the frame at both the top and bottom of the door.

Be careful not to damage the connectors by

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