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  • Page 1

    The Right Solution:

    The Agilent 1200 Infinity

    Series for LC Analysis

    Laura Phillips

    Application Scientist

    Agilent Technologies

    June 27th, 2013

  • Page 2

    The Agilent 1200 Infinity Series Where Do We Come From?

    June 27, 2013

    1100 Bin/Quat

    HPLC

    Bin (HPM)

    Quat (LPM)

    400 bar

    5 mL/min

    1290 Infinity (2009)

    UHPLC

    Bin (HPM)

    Quat (LPM - 2012)

    1200 bar

    5 mL/min

    1260 Infinity Bin/Quat

    UHPLC

    Bin (HPM) 5mL/min

    Quat (LPM) 10mL/min

    600 bar

    Price ~ 1200 Bin/Quat

    1220 Infinity

    UHPLC

    Bin (LPM)

    600 bar, 5 mL/min

    Price < 1200 Quat

    1200 Infinity Series

    1200 Bin/Quat

    HPLC

    Bin (HPM)

    Quat (LPM)

    400 bar

    5 mL/min

    1200 RRLC

    UHPLC

    Bin (HPM)

    600 bar

    5 mL/min

    Price: 1200 Bin+20%

    1200 Series (2006 2010)

    1100 Series (1995 2006)

  • Page 3

    The Agilent 1200 Infinity Series Raising the Standard in HPLC UHPLC for EVERY Lab

    June 27, 2013

    1200 Infinity Series

    1220 Infinity

    Isocratic, manual

    Gradient, manual

    Gradient, automated

    Gradient-DAD

    1260 Infinity

    Low-flow

    Cap.LC

    Nano-LC

    Chip-LC

    Analytical flow

    Binary

    Quaternary

    Isocratic

    Binary-SFC

    Quaternary Bio-Inert

    Prep-flow

    1290 Infinity

    Binary

    Quaternary

    new

    new

  • Page 4

    The Agilent 1200 Infinity Series Raising the Standard in HPLC UHPLC for EVERY Lab

    June 27, 2013

    Method Developement

    Solution

    Multi-method Solution

    High-Dynamic Range DAD

    2D-LC Solution

    Online-SPE Valve solutions Bioinert

    solutions SFC solutions

    GPC solutions

  • Page 5

    1220 Infinity LC A solution to all your needs

    Dual gradient pump with integrated degasser, 600 bar, up to 10 mL/ min, for

    flexible and reliable analysis

    Vial sampler with injector programming for

    higher automation

    80C oven for one 25 cm column for better

    results

    Variable wavelength or diode array

    detector with 80 Hz sampling rate for high

    sensitivity

    June 27, 2013

    Easy: Small footprint, low height. One

    cable.

    Affordable with ChemStation/ EZChrom

    VL software

    Expandable: Any other module can be

    added

  • Page 6

    600 bar for HPLC and UHPLC

    June 27, 2013

    Same as with the 1260 Infinity LCs, existing conventional HPLC methods

    from 1100/1200 Series LC can be run on the 1220 Infinity LC and giving the

    same results as well as UHPLC methods on 3.0mm and 4.6mmID columns

    can be run. Both with excellent performance!

    Saving half of the time by transferring a HPLC

    method to UHPLC, without loss of resolution. Excellent retention and area RSDs even under

    UHPLC conditions.

  • Page 7

    Expandability

    June 27, 2013

    External tray for walk-up:

    Together with Agilents EasyAccess software the 1220 Infinity LC with external

    tray is a perfect solution for walk-up environments!

    Additional detectors:

    Almost all 1260/1290 Infinity detectors and SQ-MS can be added!

  • Page 8

    Innovation: 1220 Infinity Mobile LC Solution Affordable, Flexible, Compatible Everywhere!

    June 27, 2013

    Instrument Control Framework

    Boost Performance

    Complete UHPLC system for

    higher resolution and shorter

    run times

    Save Time

    Bring your laboratory to the

    samples

    Reduce Costs

    Most affordable system

    giving you access to UHPLC

    technology

  • Page 9

    1260 Infinity LC

    1260/1290 Valve Solutions 1260 Infinity Bio-Inert LC Buffer Advisor

    1260 Infinity SFC

    1260 Infinity GPC

    1260/1290 ELSD

    June 27, 2013

  • Page 10

    1200 Infinity Series valve solutions

  • Page 11

    NEW Quick-Change Valve heads

    for universal Valve Drive

    1290 Infinity TCC 1290 Infinity Flexible Cube 1290 Infinity Valve Drive

    Types 600 bar, 1200bar

    Std, Bio-inert, -flow, prep

    2pos/4port duo

    2pos/ 6port

    2pos/10port

    4pos/10port

    6pos/14port

    8pos/ 9port

    12pos/13port

    6pos/ 7port

    2pos/ 8port

    2pos/12port

    Custom Valves

    Applications Multi-method/Method Dev

    Column switching (2,4,6,8)

    Solvent selection (13 26)

    Matrix-stripping

    Sample Enrichment

    Online-SPE

    Detector selection

    Heart Cutting 2DLC

    Comprehensive 2DLC

    Alternating Column Regen.

    Process Control/ Sample

    Sampling/ Fractionation

    Column Connection

    The largest and most flexible valve portfolio Workflow- and Application-Automation Flexibility

    July 30, 2013 11

  • Page 12

    Automated Column Regeneration 2-position / 10-port valve head

    1x 1290 Infinity

    Thermostatted column

    compartment (or 1290 Infinity

    Valve Drive)

    1x 2-pos/10-port valve head

    While the analysis is running

    on column 1, column 2 is

    regenerated using the flow

    from a second pump

    After the analysis on column

    1, the flow switches to

    column 2 for analysis while

    column 1 is regenerated.

  • Page 13

    Sample Enrichment 2-position / 6-port valve head

    1x 1290 Infinity

    Thermostatted column

    compartment (or 1290 Infinity

    Valve Drive)

    1x 2-pos/6-port valve head

    The analyte is retained and

    concentrated on an

    enrichment column while the

    sample matrix flows to

    waste.

    For analysis, the

    concentrated sample is

    flushed out of the

    enrichment column to the

    analytical column.

  • Page 14

    Sample Cleanup 2-position / 6-port valve head

    1x 1290 Infinity

    Thermostatted column

    compartment (or 1290 Infinity

    Valve Drive)

    1x 2-pos/6-port valve head

    Matrix components are

    retained on a pre-column

    while the analytes pass

    through to the analytical

    column for separation.

    After sample cleanup or

    matrix stripping, the

    precolumn is flushed in

    reversed flow direction by

    the second pump.

  • Page 15

    Column Selection for Method Development 6-position / 14-port valve head

    1x 1290 Infinity

    Thermostatted column

    compartment

    1x 6-pos/14-port valve

    head

    Up to 6 columns (4 columns recommended because of

    available space)

  • Page 16

    Solvent Selection for Method Development 12-position selection valve head

    Typically used with 1290 Infinity Valve Drive.

    For Method Development systems:

    1290 Infinity Valve Drive and new 12-position

    selection valve head is fully supported

    as pump-valve cluster and by Agilent

    ChemStation Method Scouting Wizard

  • Page 17

    1260 Infinity Bio-Inert LC

    June 27, 2013

  • Page 18

    Innovation: 1260 Infinity Bio-inert LC Truly Bio-inert UHPLC System

    June 27, 2013

    1260 Infinity Bio-inert LC

    Boost Performance

    Highly versatile (U)HPLC

    system for small- and large-

    molecule analysis

    Save Time

    Fast switching between bio-

    and non-bio applications,

    including cleaning

    Reduce Costs

    Only one system to maintain

    for bio- and non-bio analysis

    Stainless steel system

    1260 Infinity Bio-inert LC

  • Page 19

    New Capillary Design Ensures Bio-inertness

    June 27, 2013

    Capillaries

    Metal clad PEEK capillary

    Newest fitting design: Hybrid technique

    New capillary technolgy enables

    600 bar AND is completely metal free !!

  • Page 20

    June 27, 2013

    Buffer Advisor Software Facilitates Workflows in Ion Exchange Chromatography

    Buffer Advisor helps to:

    1. Define stock solutions for salt and pH

    gradients

    2. Check if the buffer system is suitable

    for your separation task

    3. Create a corrected time table for a

    salt- or pH gradient.

    Manual preparation:

    1. Tedious

    2. Error-prone

    3. Irreproducible (operator)

    Part of Bio-inert pump,

    G5611A

  • Page 21

    Buffer Advisor User Interface

    Area 1: Select Buffer and

    Gradient Type

    June 27, 2013

    Area 2: Enter Gradient

    Time Table

    Area 4: Review

    Composition of Stock

    Solutions and Adjust

    Area 3: Gradient Display

    Section: Process

    Gradient and Review

    Optimized Gradient with

    Compensation Steps

  • Page 22

    1260 Infinity SFC

    June 27, 2013

  • Page 23

    Innovation: Hybrid SFC/UHPLC System Double your Discovery

    June 27, 2013

    Hybrid SFC/UHPLC System

    Boost Performance

    SFC with LC-like sensitivity,

    unique 600 bar x 5 mL/min

    power range

    Save Time

    Efficient instrument utilization

    and orthogonality through

    unique switching capability

    between LC and SFC

    Reduce Costs

    Lower solvent expenses, less

    waste, and only one system

    to maintain. Upgrade from LC

    to SFC

  • Page 24

    Agilent

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