The Right Solution: The Agilent 1200 Infinity Series for ... Right Solution: The Agilent 1200 Infinity Series for LC ... 1290 Infinity TCC 1290 Infinity Flexible Cube 1290 Infinity ... served me well.

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<ul><li><p>Page 1 </p><p>The Right Solution: </p><p>The Agilent 1200 Infinity </p><p>Series for LC Analysis </p><p>Laura Phillips </p><p>Application Scientist </p><p>Agilent Technologies </p><p>June 27th, 2013 </p></li><li><p>Page 2 </p><p>The Agilent 1200 Infinity Series Where Do We Come From? </p><p>June 27, 2013 </p><p>1100 Bin/Quat </p><p>HPLC </p><p>Bin (HPM) </p><p>Quat (LPM) </p><p>400 bar </p><p>5 mL/min </p><p>1290 Infinity (2009) </p><p>UHPLC </p><p>Bin (HPM) </p><p>Quat (LPM - 2012) </p><p>1200 bar </p><p>5 mL/min </p><p>1260 Infinity Bin/Quat </p><p>UHPLC </p><p>Bin (HPM) 5mL/min </p><p>Quat (LPM) 10mL/min </p><p>600 bar </p><p>Price ~ 1200 Bin/Quat </p><p>1220 Infinity </p><p>UHPLC </p><p>Bin (LPM) </p><p>600 bar, 5 mL/min </p><p>Price &lt; 1200 Quat </p><p>1200 Infinity Series </p><p>1200 Bin/Quat </p><p>HPLC </p><p>Bin (HPM) </p><p>Quat (LPM) </p><p>400 bar </p><p>5 mL/min </p><p>1200 RRLC </p><p>UHPLC </p><p>Bin (HPM) </p><p>600 bar </p><p>5 mL/min </p><p>Price: 1200 Bin+20% </p><p>1200 Series (2006 2010) </p><p>1100 Series (1995 2006) </p></li><li><p>Page 3 </p><p>The Agilent 1200 Infinity Series Raising the Standard in HPLC UHPLC for EVERY Lab </p><p>June 27, 2013 </p><p>1200 Infinity Series </p><p>1220 Infinity </p><p>Isocratic, manual </p><p>Gradient, manual </p><p>Gradient, automated </p><p>Gradient-DAD </p><p>1260 Infinity </p><p>Low-flow </p><p>Cap.LC </p><p>Nano-LC </p><p>Chip-LC </p><p>Analytical flow </p><p>Binary </p><p>Quaternary </p><p>Isocratic </p><p>Binary-SFC </p><p>Quaternary Bio-Inert </p><p>Prep-flow </p><p>1290 Infinity </p><p>Binary </p><p>Quaternary </p><p>new </p><p>new </p></li><li><p>Page 4 </p><p>The Agilent 1200 Infinity Series Raising the Standard in HPLC UHPLC for EVERY Lab </p><p>June 27, 2013 </p><p>Method Developement </p><p>Solution </p><p>Multi-method Solution </p><p>High-Dynamic Range DAD </p><p>2D-LC Solution </p><p>Online-SPE Valve solutions Bioinert </p><p>solutions SFC solutions </p><p>GPC solutions </p></li><li><p>Page 5 </p><p>1220 Infinity LC A solution to all your needs </p><p> Dual gradient pump with integrated degasser, 600 bar, up to 10 mL/ min, for </p><p>flexible and reliable analysis </p><p> Vial sampler with injector programming for </p><p>higher automation </p><p> 80C oven for one 25 cm column for better </p><p>results </p><p> Variable wavelength or diode array </p><p>detector with 80 Hz sampling rate for high </p><p>sensitivity </p><p>June 27, 2013 </p><p> Easy: Small footprint, low height. One </p><p>cable. </p><p> Affordable with ChemStation/ EZChrom </p><p>VL software </p><p> Expandable: Any other module can be </p><p>added </p></li><li><p>Page 6 </p><p>600 bar for HPLC and UHPLC </p><p>June 27, 2013 </p><p>Same as with the 1260 Infinity LCs, existing conventional HPLC methods </p><p>from 1100/1200 Series LC can be run on the 1220 Infinity LC and giving the </p><p>same results as well as UHPLC methods on 3.0mm and 4.6mmID columns </p><p>can be run. Both with excellent performance! </p><p>Saving half of the time by transferring a HPLC </p><p>method to UHPLC, without loss of resolution. Excellent retention and area RSDs even under </p><p>UHPLC conditions. </p></li><li><p>Page 7 </p><p>Expandability </p><p>June 27, 2013 </p><p>External tray for walk-up: </p><p>Together with Agilents EasyAccess software the 1220 Infinity LC with external </p><p>tray is a perfect solution for walk-up environments! </p><p>Additional detectors: </p><p>Almost all 1260/1290 Infinity detectors and SQ-MS can be added! </p></li><li><p>Page 8 </p><p>Innovation: 1220 Infinity Mobile LC Solution Affordable, Flexible, Compatible Everywhere! </p><p>June 27, 2013 </p><p>Instrument Control Framework </p><p> Boost Performance </p><p>Complete UHPLC system for </p><p>higher resolution and shorter </p><p>run times </p><p> Save Time </p><p>Bring your laboratory to the </p><p>samples </p><p> Reduce Costs </p><p>Most affordable system </p><p>giving you access to UHPLC </p><p>technology </p></li><li><p>Page 9 </p><p>1260 Infinity LC </p><p> 1260/1290 Valve Solutions 1260 Infinity Bio-Inert LC Buffer Advisor </p><p> 1260 Infinity SFC </p><p> 1260 Infinity GPC </p><p> 1260/1290 ELSD </p><p>June 27, 2013 </p></li><li><p>Page 10 </p><p>1200 Infinity Series valve solutions </p></li><li><p>Page 11 </p><p>NEW Quick-Change Valve heads </p><p>for universal Valve Drive </p><p> 1290 Infinity TCC 1290 Infinity Flexible Cube 1290 Infinity Valve Drive </p><p>Types 600 bar, 1200bar </p><p>Std, Bio-inert, -flow, prep </p><p> 2pos/4port duo </p><p>2pos/ 6port </p><p> 2pos/10port </p><p> 4pos/10port </p><p> 6pos/14port </p><p> 8pos/ 9port </p><p>12pos/13port </p><p>6pos/ 7port </p><p>2pos/ 8port </p><p>2pos/12port </p><p>Custom Valves </p><p>Applications Multi-method/Method Dev </p><p> Column switching (2,4,6,8) </p><p> Solvent selection (13 26) </p><p> Matrix-stripping </p><p> Sample Enrichment </p><p> Online-SPE </p><p> Detector selection </p><p> Heart Cutting 2DLC </p><p> Comprehensive 2DLC </p><p> Alternating Column Regen. </p><p> Process Control/ Sample </p><p>Sampling/ Fractionation </p><p> Column Connection </p><p>The largest and most flexible valve portfolio Workflow- and Application-Automation Flexibility </p><p>July 30, 2013 11 </p></li><li><p>Page 12 </p><p>Automated Column Regeneration 2-position / 10-port valve head </p><p> 1x 1290 Infinity </p><p>Thermostatted column </p><p>compartment (or 1290 Infinity </p><p>Valve Drive) </p><p> 1x 2-pos/10-port valve head </p><p> While the analysis is running </p><p>on column 1, column 2 is </p><p>regenerated using the flow </p><p>from a second pump </p><p> After the analysis on column </p><p>1, the flow switches to </p><p>column 2 for analysis while </p><p>column 1 is regenerated. </p></li><li><p>Page 13 </p><p>Sample Enrichment 2-position / 6-port valve head </p><p> 1x 1290 Infinity </p><p>Thermostatted column </p><p>compartment (or 1290 Infinity </p><p>Valve Drive) </p><p> 1x 2-pos/6-port valve head </p><p> The analyte is retained and </p><p>concentrated on an </p><p>enrichment column while the </p><p>sample matrix flows to </p><p>waste. </p><p> For analysis, the </p><p>concentrated sample is </p><p>flushed out of the </p><p>enrichment column to the </p><p>analytical column. </p></li><li><p>Page 14 </p><p>Sample Cleanup 2-position / 6-port valve head </p><p> 1x 1290 Infinity </p><p>Thermostatted column </p><p>compartment (or 1290 Infinity </p><p>Valve Drive) </p><p> 1x 2-pos/6-port valve head </p><p> Matrix components are </p><p>retained on a pre-column </p><p>while the analytes pass </p><p>through to the analytical </p><p>column for separation. </p><p> After sample cleanup or </p><p>matrix stripping, the </p><p>precolumn is flushed in </p><p>reversed flow direction by </p><p>the second pump. </p></li><li><p>Page 15 </p><p>Column Selection for Method Development 6-position / 14-port valve head </p><p> 1x 1290 Infinity </p><p>Thermostatted column </p><p>compartment </p><p> 1x 6-pos/14-port valve </p><p>head </p><p> Up to 6 columns (4 columns recommended because of </p><p>available space) </p></li><li><p>Page 16 </p><p>Solvent Selection for Method Development 12-position selection valve head </p><p>Typically used with 1290 Infinity Valve Drive. </p><p>For Method Development systems: </p><p>1290 Infinity Valve Drive and new 12-position </p><p>selection valve head is fully supported </p><p>as pump-valve cluster and by Agilent </p><p>ChemStation Method Scouting Wizard </p></li><li><p>Page 17 </p><p>1260 Infinity Bio-Inert LC </p><p>June 27, 2013 </p></li><li><p>Page 18 </p><p>Innovation: 1260 Infinity Bio-inert LC Truly Bio-inert UHPLC System </p><p>June 27, 2013 </p><p>1260 Infinity Bio-inert LC </p><p> Boost Performance </p><p>Highly versatile (U)HPLC </p><p>system for small- and large-</p><p>molecule analysis </p><p> Save Time </p><p>Fast switching between bio- </p><p>and non-bio applications, </p><p>including cleaning </p><p> Reduce Costs </p><p>Only one system to maintain </p><p>for bio- and non-bio analysis </p><p>Stainless steel system </p><p>1260 Infinity Bio-inert LC </p></li><li><p>Page 19 </p><p>New Capillary Design Ensures Bio-inertness </p><p>June 27, 2013 </p><p>Capillaries </p><p>Metal clad PEEK capillary </p><p>Newest fitting design: Hybrid technique </p><p>New capillary technolgy enables </p><p>600 bar AND is completely metal free !! </p></li><li><p>Page 20 </p><p>June 27, 2013 </p><p>Buffer Advisor Software Facilitates Workflows in Ion Exchange Chromatography </p><p>Buffer Advisor helps to: </p><p>1. Define stock solutions for salt and pH </p><p>gradients </p><p>2. Check if the buffer system is suitable </p><p>for your separation task </p><p>3. Create a corrected time table for a </p><p>salt- or pH gradient. </p><p>Manual preparation: </p><p>1. Tedious </p><p>2. Error-prone </p><p>3. Irreproducible (operator) </p><p>Part of Bio-inert pump, </p><p>G5611A </p></li><li><p>Page 21 </p><p>Buffer Advisor User Interface </p><p>Area 1: Select Buffer and </p><p>Gradient Type </p><p>June 27, 2013 </p><p>Area 2: Enter Gradient </p><p>Time Table </p><p>Area 4: Review </p><p>Composition of Stock </p><p>Solutions and Adjust </p><p>Area 3: Gradient Display </p><p>Section: Process </p><p>Gradient and Review </p><p>Optimized Gradient with </p><p>Compensation Steps </p></li><li><p>Page 22 </p><p>1260 Infinity SFC </p><p>June 27, 2013 </p></li><li><p>Page 23 </p><p>Innovation: Hybrid SFC/UHPLC System Double your Discovery </p><p>June 27, 2013 </p><p>Hybrid SFC/UHPLC System </p><p> Boost Performance </p><p>SFC with LC-like sensitivity, </p><p>unique 600 bar x 5 mL/min </p><p>power range </p><p> Save Time </p><p>Efficient instrument utilization </p><p>and orthogonality through </p><p>unique switching capability </p><p>between LC and SFC </p><p> Reduce Costs </p><p>Lower solvent expenses, less </p><p>waste, and only one system </p><p>to maintain. Upgrade from LC </p><p>to SFC </p></li><li><p>Page 24 </p><p>Agilent 1260 Infinity Analytical SFC/MS System 1260 Infinity SFC and the G6100 SQ Mass Spectrometer </p><p>June 27, 2013 </p><p>Analysis of leachables in </p><p>food packaging material by </p><p>SFC/MS </p></li><li><p>Page 25 </p><p>1260 Infinity GPC </p><p>June 27, 2013 </p></li><li><p>Page 26 </p><p>High Performance GPC/SEC for Routine Work </p><p>June 27, 2013 </p><p>High Performance GPC/SEC Solution </p><p> Boost Performance </p><p>High performance pump &amp; oven </p><p>for reproducible molecular </p><p>weights </p><p> Save Time </p><p>RID warm up less than 1 hour, </p><p>complete system based on </p><p>industry-leading Agilent LC </p><p>systems for maximum uptime </p><p> Reduce Costs </p><p>RID with built-in solvent recycle, </p><p>minimum training effort. </p><p>I have always been impressed by the </p><p>quality, robustness, reliability and ease-</p><p>of-use of their products, which have </p><p>served me well. - John P. ODonnell, </p><p>Research Scientist, FUJIFILM Imaging Colorants </p><p>Ltd., Manchester, UK </p></li><li><p>Page 27 </p><p>1260 Infinity GPC/SEC Multi Detector Suite Summary </p><p>June 27, 2013 </p><p>Key Benefits </p><p>Comprehensive Allows complex multi detector GPC </p><p>experiments to be performed routinely. </p><p>Versatile The MDS can be controlled by software </p><p>or the keypad for maximum ease of </p><p>use, and can be used with any existing </p><p>modular or integrated HPLC system. </p><p>Convenient Data from an existing detector can be </p><p>collected and processed through the </p><p>MDS. </p><p>High Quality </p><p>Results </p><p>The detector cells are heated for </p><p>optimum baseline stability and </p><p>minimum instrument downtime. </p><p>Adaptable Can be expanded at any time to </p><p>include additional detectors. </p><p>Safe Incorporates multiple solvent leak </p><p>detectors to prevent spillage of </p><p>potentially hazardous solvents. </p></li><li><p>Page 28 </p><p>Agilent 1260 Infinity ELSD </p><p>&amp; 1290 Infinity ELSD </p><p>Evaporative Light Scattering </p><p>Detection (ELSD) </p></li><li><p>Page 29 </p><p>Introduction: ELSD General Benefits </p><p>June 27, 2013 </p><p> Universal detection </p><p> More uniform response </p><p> Gradient Compatible </p><p> Complimentary to LC-MS </p><p> Eliminates derivatization steps in analytical methods </p></li><li><p>Page 30 </p><p>Agilent ELSD Portfolio </p><p>June 27, 2013 </p><p>G4260B G4261B </p><p>1260 Infinity ELSD </p><p>1290 Infinity ELSD </p><p>(Cooling deleted) </p><p>1290 Infinity ELSD </p><p>(Standard, cooled) </p><p>Light Source Blue LED (480nm) Blue Laser (405nm) </p><p>Temperature </p><p>range </p><p>Nebulizer: 25-90C </p><p>Evaporator: ambient 120C </p><p>Nebulizer: 25-90C </p><p>Evaporator: ambient 120C </p><p>Nebulizer: 25-90C </p><p>Evaporator: 10C 80C </p><p>Features </p><p> Hydrocortisone limit of detection </p><p>30ng </p><p> RS232 Communication only </p><p> Hydrocortisone limit of detection 4ng </p><p> 5-9 x improvement in sensitivity over current model </p><p> Unique sub-ambient operation (greater response for volatile </p><p>compounds) </p><p> RS232 and LAN Communication </p><p> Voltage selection (110/240V) no longer required </p><p> Variable gas flow for uniformity of response </p><p> Fast data collection (up to 80Hz) </p><p> Controlled via ChemStation / EZChrom </p><p> Front Panel control of all parameters (Temperature, gas flow, data rate etc) </p></li><li><p>Page 31 </p><p>Increased sensitivity, reduced baseline noise </p><p>June 27, 2013 </p><p>The increased laser intensity </p><p>coupled with a high gain </p><p>photomultiplier and digital signal </p><p>processing increases signal and </p><p>reduces noise. </p><p>G4261B </p><p>S/N 158 </p><p>G4260B </p><p>S/N = 5 </p><p>Injection: 10L (Hydrocortisone 50ng-on column) </p><p>Column: Zorbax Eclipse XDB-C18 (5m, 4.6x150mm) </p><p>Mobile Phase: Water/ACN (80/20) </p><p>Flow Rate: 1.0 m/min </p><p>ELSD: Neb 40C, Evap 60C, Gas 1.05SLM </p><p>Signal/noise calculated using OpenLab Chemstation v1.04 </p></li><li><p>Page 32 </p><p>Triglycerides in Natural Oils </p><p>June 27, 2013 </p><p>Sample: 2mg/ml Starflower Oil </p><p>Column: C18 5m, 250x4.6mm </p><p>Eluent A: ACN </p><p>Eluent B: DCM </p><p>Gradient: 30-50% B in 40 mins; 50-90% in 2 </p><p>mins, hold for 3mins </p><p>Flow Rate: 1.0ml/min </p><p>Inj Vol: 20l </p><p>Detector: neb=40C, evap=70C, gas=1.4 SLM </p></li><li><p>Page 33 </p><p>Environmental Application: </p><p>Characterizing Biodiesel </p><p>June 27, 2013 </p><p>Column: BDS Hypersil Cyano 3m, 250x4.6 mm </p><p>Eluent A: 0.4% Acetic acid in Hexane </p><p>Eluent B: 0.4% Acetic acid in Methyl tert-Butyl Ether (MTBE) </p><p>Gradient: 100% A, hold 5 mins; 0-80% B in 10 mins, </p><p> hold 2 mins; 80-0%B in 3 mins </p><p>Flow Rate: 1.0 ml/min </p><p>Inj Vol: 20l </p><p>Detector: neb=40C, evap=80C, gas=0.8SLM </p></li><li><p>Page 34 </p><p>1290 Infinity LC </p><p> 1290 Infinity Quaternary Pump 1290 2D LC </p><p> 1290 Infinity with ISET </p><p> June 27, 2013 </p></li><li><p>Page 35 </p><p>1290 Infinity Quaternary LC </p><p>June 27, 2013 </p></li><li><p>Page 36 </p><p>Innovation: 1290 Infinity Quaternary Pump Quaternary Pump with Binary Pump-like Performance </p><p>June 27, 2013 </p><p>1290 Infinity Quaternary Pump </p><p> Boost Performance </p><p>Highest, binary-like accuracy </p><p>and precision for composition </p><p>and flow, with exceptionally low </p><p>delay volume </p><p> Save Time </p><p>Accelerates method transfer </p><p>from R+D to QA and from </p><p>HPLC to UHPLC </p><p> Reduce Costs </p><p>One pump for any applications: </p><p>Most universal at HPLC-like </p><p>costs of ownership </p></li><li><p>Page 37 </p><p>Power Range </p><p> 1200bar up to 2mL/min </p><p> 800bar at 5mL/min </p><p> For any kind of analysis </p><p>Flow Accuracy and Precision </p><p>&lt; 0.07 % RSD or 0.01 min SD </p><p> 1.0 % or 10 L </p><p> High RT precision in </p><p>isocratic runs </p><p>Composition Accuracy and Precision </p><p>&lt; 0.15 % RSD or 0.02 min SD </p><p> 0.4 % (1-99 % Composition B) </p><p> High RT precision in </p><p>gradient runs </p><p>Agilent 1290 Infinity Quaternary Pump Specifications &amp; Benefits </p><p>Composition Range </p><p>1-99 % </p><p> Wide analytical range </p><p>Delay Volume </p><p> &lt; 350 L </p><p> For fast quaternary gradients </p></li><li><p>Page 38 </p><p>1290 Infinity 2D-LC Solution </p><p>June 27, 2013 </p></li><li><p>Page 39 </p><p>Innovation: 1290 Infinity 2D-LC Solution Square your Separation </p><p>June 27, 2013 </p><p>1290 Infinity 2D-LC Solution </p><p> Boost Performance </p><p>New levels of separation power, </p><p>detection sensitivity and </p><p>precision in 2D-LC </p><p> Save Time </p><p>A single ultra high peak </p><p>capacity run instead of many </p><p>conventional runs with 1min </p><p>method setup! </p><p> Reduce Costs </p><p>Special features to reduce </p><p>solvent and valve cost, upgrade </p><p>paths for existing LC-systems </p></li><li><p>Page 40 </p><p>Data Analysis </p><p>June 27, 2013 </p><p>For heart-cutting 2D-LC by Agilents OpenLAB CDS ChemStation ed. </p><p>For comprehensive 2D-LC Agilent recommends: </p><p> GC Image LCxLC Edition Software from GC Image LLC. </p><p>Key features offered: </p><p> Direct Agilent datafile import </p><p> Peak identification, integration, annotation </p><p> Comparative Analysis and Visualization </p><p> Compound-libraries </p><p> MS-data handling </p></li><li><p>Page 41 </p><p>Hardware Module-flexibility </p><p>June 27, 2013 </p><p>1. Dimension </p><p>2. Dimension </p><p>1290 Infinity </p><p>Binary Pump </p><p>1290 Infinity </p><p>Binary Pump </p><p>1290 Infinity </p><p>Autosampler or 1260 </p><p>HiP Autosampler </p><p>Optional </p><p>1260/1290 </p><p>Infinity </p><p>Detector </p><p>One or two </p><p>1290 Infinity </p><p>TCC </p><p>Optional </p><p>1260/1290 </p><p>Infinity </p><p>Detector </p><p>1260/1290 </p><p>Infinity </p><p>detector </p><p>1260 Infinity Binary </p><p>or Quaternary Pump </p><p>1260 Infinity </p><p>Capillary Pump 1260 Infinity </p><p>Autosampler </p><p>For 1st </p><p>dimension </p><p>chromatogram </p><p>and peak-</p><p>triggering </p><p>To monitor </p><p>waste-line </p><p>Almost any Agilent pump or autosampler in the 1st dimension! </p><p>Almost any detectors are supported! </p><p>A 1290 Infinity Binary Pump for the 2nd dimension is required. </p></li><li><p>Page 42 </p><p>Applications examples - components of beer samples </p><p>June 27, 2013 </p><p>Separation by RP (C18) </p><p>Sepa</p><p>ration b</p><p>y R</p><p>P (</p><p>Phe</p><p>nyl)</p><p>Analysis of two different commercially available beer samples with different </p><p>separation modes </p><p>1st dimension - Reverse Phase (C18) 2nd dimension - Reverse Phase (Phenyl) </p><p>Beer A Beer B </p></li><li><p>Page 43 </p><p>1290 Infinity Binary LC w. ISET </p></li><li><p>Page 44 </p><p>Innovation: ISET Intelligent System Emulation Technology </p><p>June 27, 2013 </p><p>Intelligent System...</p></li></ul>

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