agilent 1290 infinity lc 월등히 강력해진 성능 .1290 infinity lc 액체 이송 시스템의

Download Agilent 1290 Infinity LC 월등히 강력해진 성능 .1290 Infinity LC 액체 이송 시스템의

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  • 1290

    Agilent 1290 Infinity LC

  • 2

    Agilent 1290 Infinity LC

    Agilent 1290 Infinity LC HPLC, RRLC UHPLC

    , , , . Intelligent System Emulation Technology(ISET) 1290 Infinity LC

    HPLC UHPLC

    , .

    1290 Infinity LC LC/UV LC/MS ,, . Active damping

    Agilent Jet Weaver mixer

    (optofluidic waveguides) Agilent Max-Light

    2 mL/min 1200 bar, 5 mL/min 800 bar 1290 Infinity LC HPLC RRLC UHPLC, , , , , , . Intelligent System Emulation Technology(ISET) LC

    , carryover

    Agilent Quick-Change Ultrahigh-Throughput,

    1290 Infinity LC, . HPLC UHPLC .

    ISET 1290 Infinity LC , 1100 Binary LC delay mixing . .

    1 1.5 3 [ ]

    [mAU]

    0

    400

    200100

    100

    600700

    500

    800

    3.52 2.5

    R = 1.90S

    1100 Binary LC

    ISET 1290 Infinity LC

    R = 1.87S

  • 3

    0 1

    1200

    bar1200 Infinity

    2 3 4 5mL/min

    1000

    800

    600

    200

    400

    0

    1260 1220 Infinity LC

    1200 HPLC

    1290 Infinity LC 1200 bar 5 mL/min , , .

    Agilent 1290 Infinity www.agilent.com/chem/1290 .

    [AU]

    -6-8

    -4-202468

    A

    8 AUB

    5 AUC

    3 AU/cmD

    3 AUAgilent DAD0.6 AU/cm

    1290 Infinity

    .

    3~5

  • 4

    , ,. Agilent 1290 Infinity LC

    .

    .

    : ZORBAX SB-C18, 4.6 x 150 mm, 5 m

    : 10 L : 12 min

    : 1.5 mL/minPeak capacity: 228 W(1/2)/: 76.5

    : 78 bar

    Agilent UHPLC ! Method translator Cost saving calculator HPLC , .

    ,

    ()

    , ,

    .

    0 2 4 6 8

    4

    3

    [ ]

    1

    2

    34

    56

    7

    10

    2

    1

    0

    [mAU]

    12

    1 Uracil2 Phenol3 Methylparaben4 Ethylparaben

    5 Propylparaben6 Butylparaben7 Heptylparaben

    HPLC

    www.agilent.com/chem/hplc2uhplc .

  • 5

    0 2 3

    10

    [ ]

    1

    2

    3

    4 56

    7

    5

    0

    [mAU]

    1

    15

    4

    : ZORBAX RRHD SB-C18, 2.1 x 50 mm, 1.8 m

    : 2 L : 0.6 min

    : 2 mL/minPeak capacity: 119 W(1/2)/: 740

    : 1030 bar

    : ZORBAX RRHD SB-C18, 2.1 x 100 mm, 1.8 m

    : 2 L : 4 min

    : 1.2 mL/minPeak capacity: 306 W(1/2)/: 861

    : 1007 bar

    : ZORBAX RRHD SB-C18, 2.1 x 250 mm, 1.8 m

    : 2 L : 25 min

    : 0.4 mL/minPeak capacity: 430 W(1/2)/: 2270

    : 1020 bar

    : ZORBAX RRHD SB-C18, 2.1 x 150 mm, 1.8 m

    : 2 L : 12 min

    : 0.7 mL/minPeak capacity: 375 W(1/2)/: 784

    : 965 bar

    20

    10

    3

    Peak capacity 1.4

    11

    Peak capacity 1.6

    10

    Peak capacity 1.9

    30

    Agilent 1290 Infinity LC

    0 0.2 0.4

    252015

    [ ]

    1

    2

    3

    4 56

    7

    0.6

    1050

    [mAU]

    3035

    0 2 4 6 8

    1086

    [ ]

    1

    2

    3

    4 56

    7

    12

    4

    20

    [mAU]12

    10

    0 5 10 15 20

    60

    48

    36

    [ ]

    1

    2

    34

    56

    7

    25

    24

    12

    0

    [mAU]

    Agilent 1290 Infinity LC www.agilent.com/chem/1290 .

  • 6

    1290 Infinity LC

    UHPLC, RRLC HPLC

    .

    A

    B

    Jet WeaverMixer

    Purge

    Agilent 1290 Infinity Binary

    1200 bar 2 mL/min, 800 bar 5 mL/min

    10 L delay volume

    LC/MS LC/UV

    - active damping

    ,

    Seal

    ,

    purging priming

  • 7

    1290 Infinity Binary .

    delay volume UV Jet WeaverMixer

    Trifluoroacetic acid(TFA) Agilent Jet Weaver

    : ZORBAX RRHD SB-C18, 2.1 x 50 mm, 1.8 m

    : A: Water + 0.1 %TFAB: Acetonitrile + 0.08 %TFA

    : Isocratic: 65 %B: 1 mL/min : 254/5(400/80) nm, 80 Hz

    0.02 0.04 0.06 0.08 [ ]

    0.10.2

    [mAU]

    0.4

    1.0

    0.6

    0.8

    Jet Weaver V35 Jet Weaver

    Peak 10 [] %RSD

    1 0.451 0.01600

    2 0.551 0.00769

    3 0.617 0.00517

    4 0.667 0.00575

    5 0.685 0.00577

    6 0.713 0.00538

    7 0.761 0.00585

    8 0.818 0.00571

    9 0.890 0.00797

    0.4 0.5 0.6 0.7 0.8 [ ]

    0.9

    2000

    [mAU]

    400600800

    1000120014001600

    : ZORBAX RRHD SB-C18, 2.1 x 50 mm, 1.8 m,

    : Water/Acetonitrile: 0.5 5~95 %B

    : 1.2 mL/min,: 254/10(400/80) nm, 80 Hz

    100

    200

    300

    400

    500

    [mAU]

    3: % RSD= 0.005

    [ ]0.620.61 0.63

    .

    Agilent 1290 Infinity LC www.agilent.com/chem/1290 .

    (ripple) Restriction capillary, 50 m x 50 cm

    : Water: 1 mL/min

    Jet Weaver V35 1290 Infinity Binary(delay volume 40 L )

    Damper Mixer 1200

    Binary SL(delay volume 120 L)

    500

    510

    505

    [ ]

    49510.09.58.5 9.0 10.5

    [bar]

    505

    510

    515

    520

    [ ]10.09.58.5 9.0 10.5

    [bar]

  • 8

    1290 Infinity UHPLC HPLC .(optofluidic waveguides) Agilent Max-Light

    100%.

    (drift).

    10 mm Agilent Max-Light

    3 AU 2.1, 3.0, 4.6 mm ID

    (drift)

    160 Hz

    LC

    (1~8 nm)

    , ,

    RFID

    1200 DAD SL

    [ ]

    [mAU]

    : ZORBAX RRHT SB-C18, 2.1 x 50 mm, 1.8 m

    : A: WaterB: Acetonitrile

    : 3 5~95 %B: 0.5 mL/min: 80 C : 254/4(360/80) nm, 40 Hz

    Agilent 1290 Infinity UV

    (optofluidic waveguides) Agilent Max-Light .

  • 9

    UV 1290Infinity 60 mm AgilentMax-Light UV . 0.6 AU/cm

    1200 DAD VWD 11.

    [mAU]

    0

    10

    5

    15

    20

    25

    30

    0 1 2 3 4 5

    1200 VWD 10 mm

    1290 Infinity DAD 60 mm Max-Light

    UV anthracene.1290 Infinity DAD

    1200 DAD

    VWD 11.

    1200 VWD: < 0.44 x 10-5 AU

    Drift: < 0.99 x 10-4 AU

    1290 Infinity VWD: < 0.126 x 10-5 AU 3.5

    Drift: < 0.42 x 10-4 AU 2.4

    [mAU]

    [ ]

    1290 Infinity

    1290 Infinity 1.5 AU 1 x 10-4 AU/h (drift), . 160 Hz LC 100% .

    1290 Infinity VWD

    1200 VWD

    1290 Infinity DAD 1200 DAD 1100 VWD60 mm 10 mm 10 mm

    [mAU] 28.88 4.938 4.800

    [AU] 9.806 19.08 18.94

    / 2944 259 253

    Agilent 1290 Infinity LC www.agilent.com/chem/1290 .

    : ZORBAX SB-C18, 150 x 4.6 mm, 5 m

    : 5 L(anthracene 835 pg/L): A: Water

    B: Acetonitrile: Isocratic: 80 %B: 1.5 mL/min: DAD: 251/4 nm, ref. 450/80 nm,

    4 nm, 2.5 HzVWD: 251 nm, 2.5 Hz

    : Restriction capillary: Water: 1 mL/min

    : 36 C: 254 nm

    : 2: > 0.1

  • 10

    1290 Infinity LC

    . (ISET) LC

    .

    , carryover . Agilent Quick- Change Ultrahigh Throughput, (multi-methods)

    .

    ,

    1290 Infinity LC Injector HTC HTS 24 microtiter plates 1290Infinity LC Injector High-Throughput . 1200 bar(dynamic load) , carryover(0.004 % chlorhexidine).

    Agilent 1290 Infinity LC, ,, . .

    0

    1200

    bar

    2 3 4 5mL/min

    1000

    800

    600

    200

    400

    0

    * : 250, 150, 100, 50 mm : Water/Acetonitrile (40 C)

    1

    Poroshell 120 2.7 m

    2.1 mm ID* 3.0 mm ID* 4.6 mm ID*

    ZORBAX RRHD 1.8 m

    ZORBAX RRHD Poroshell 120

    1.8 m 2.7 m

    (ISET)

    ISET 1290 Infinity LC HPLC UHPLC .

    . LC .

    ISET HPLC , UHPLC . LC .

    UHPLC . .

    Time

    Response

    90%

    10%

    1290 Infinity LCISET 1290 Infinity1100 Binary

    ISET

    ISET 1290 Infinity LC delay volume 1100 Binary LC . .

  • 11

    Agilent Quick-Change , RFID , , .

    1290 Infinity Column Compartment1290 Infinity Column Compartment.

    0.05 C

    Peltier 10 C 100 C,

    30 cm 3 30 cm

    -

    Agilent Quick-Change (Multi-method), 2 3. 6 6 8. Quick-Change

    .

    Agilent 1290 Infinity LC www.agilent.com/chem/1290 .

    1290 Infinity flow-through 1290 Infinity

    .

    0.1~40 L

    (overlap)

    carryover seal needle seat (0.004 % chlorhexidine).

    carry over 1290 Infinity Flexible Cubeneedle seat (0.001% chlorhexidine).

    MeteringdeviceFlush

    port

  • 12

    1290 Infinity LC. ,. HPLC

    UHPLC .

    1290 Infinity LC Agilent 1100 LC. 1290 Infinity LC LC.

    Agilent Lab Advisor(early maintenancefeedback, EMF). , , (metering device), , RFID

    . .

    Agilent Lab Advisor. .

    Agilent Lab Advisor , .

    , (metering device), needle seat .

    4

    0.80.6

    0.2

    115 6 10987

    1.4

    1.0

    0.4

    1.2

    1290 Infinity LC1100 LC

    Meteringdevice

    leak

    leak

    leak

    1100 LC 1290 Infinity LC (QI*) (*QI ).

  • 13

    Agilent Remote AdvisorAgilent Remote Advisor. Remote Advisor

    , .

    seal. 1290Infinity Binary . stroke (adaptive)

    Agilent 1290 Infinity LC www.agilent.com/chem/1290 .

    Remote Advisor IT , IT . , 10

    6 Remote Advisor .

    , . 1290 Infinity LC 95 %.

    . seal, .

    1290 Infinity Binary

    .

    Agilent 1200 Infinity LC . 1220Infinity LC, 1260Infinity LC 1290 InfinityLC, . 1200 Infinity LC 3~5.*

    3~5

    * .

  • 14

    Agilent 1290 Infinity LC Agilent 6000 LC/MS

    .

    , .

    LC/MS .

    2 mL/min 1200 bar

    Agilent TOF Q-TOF

    delay volume

    iFunnel 6490 Triple Quadrupole MS 1290 LC

    carry-over

    LC/MS

    250 90

    1290 Infini

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