Status ReportDNA Barcoding in Argentina

Pablo Luis TubaroMuseo Argentino de Ciencias Naturales “Bernardino Rivadavia”

iBOL Argentina Fund

Five calls for projects from 2008 to 2012, 110 projects supported.

The structure of the iBOL project in ArgentinaiBOL Argentina Committee

Five Reference Barcode Labs.

Leading Labs Training Workshops for DNA Barcoding.

iBOL coordinator

Cruises of the Oceanographic vessel

Structure of Argentinian barcoding network

MACN – Buenos Aires

Nágera Biological Station Mar del Plata

INIBIOMA - Bariloche

CENPAT – Puerto Madryn

IBONE - Corrientes

How Barcode Network works in Argentina

Projects supported byiBOL Argentina Fund

Projects supported byiBOL Argentina Fund

BIO/CCDBLysis plates

PCR Plates (no permits required)

Exporting permits (Fauna, Acceso Rec. Genéticos 226/2010)

Five ReferenceBarcode LabsCONICETLlabs. Training Workshops atMACN

Barcodes

Projects supported byiBOL Argentina Fund

Building the DNA barcode library of Argentinian species

The projects supported by iBOL Argentina Fund

• The researchers must be principal investigators of a PIP or a PICT.

• Tissues and vouchers must be stored in permanent collections.

• Tissues and vouchers must be compliant with barcode standards.

• The funds support only the extra costs of preserving tissues and vouchers, including small equipments, technical services, etc. Only small amounts are allowed to support field work. No PCR and sequencing costs are supported. • Researchers and their fellows have to take the Leading Labs Training Workshop for DNA barcoding.

• Specimens must be collected following legal norms.

• Researchers have to send the tissue samples to CONICET – iBOL Labs. to produce their barcode sequences.

Leading Labs Training Workshop for DNA Barcoding - MACN

“Puerto Deseado” cruises to get samples for iBOL projects

The iBOL-IDRC project

Objetives:

Engaging Developing Nations in the International Barode of Life Project (iBOL)

• To support some key elements of the iBOL project structure.

• To create barcode libraries that allow practical applications in the participating

countries.• To secure the active participation of five developing nations in the iBOL project.

• To develop an ABS policy for the iBOL project.

ActinopterygiiUnited States 12810 Argentina 2184 China 1364Australia 9033 Philippines 2130 Panama 1295Mexico 6527 India 2048 Malaysia 1166Brazil 6444 Taiwan 1680 Madagascar 885Canada 6292 Kenya 1642 New Caledonia 747Indonesia 3520 France 1607 Seychelles 740South Africa 2238 French Polynesia 1524

128.914 barcodes belonging to 13.183 species

Canada 4992 Brazil 1741 Bolivia 555Peru 3491 Japan 1399 Australia 538Argentina 3195 Indonesia 1109 Sweden 449Panama 2046 Papua New Guinea 695 Netherlands 448United States 1873 New Zealand 642 Philippines 336Mexico 1788 Norway 631 Spain 318Russia 1756 Ecuador 612

Aves 32030 barcodes belonging to 4563 species

Costa Rica 157609 Germany 12133 South Africa 6400Australia 106719 China 10924 Panama 5823Canada 98213 Brazil 10141 Argentina 5466United States 90582 Peru 9807 Finland 4519Papua New Guinea 19524 Indonesia 8028 United Kingdom 4295Mexico 15256 Italy 7380 Russia 4287Ecuador 12961 Spain 7141

Lepidoptera 714.950 barcodes belonging to 73.377 species

Canada 2565 Thailand 463 Panama 160Costa Rica 1583 Brazil 453 Colombia 121United States 1459 Bolivia 266 India 114Argentina 743 South Africa 239 Peru 111Kenya 688 Guatemala 232 Chile 104Germany 678 Russia 230 Norway 99Mexico 613 Australia 185

Apidae 12.311 barcodes belonging to 2021 species

Barcode Argentine Butterflies (ARLEP) and Moths (ARMOT)

ARMOT

Emerging Leaders in the Americas Program - ELAP

PLO0308AAA9670

11.7% COIdivergence

4.7% COIdivergence

12.2 COIdivergence

PLO0104ACA8897

PLO0104AAA9669

Finding a deletion

Building the Biodiversity Macroscope in Bolivia

CIAR – Pcía de Misiones – Selva subtropical

Ea. El Bagual – Pcia de Formosa – Chaco húmedo

Malaise 1: 27.44476ºS 54.94032ºOMalaise 2: 27.44300ºS 54.94237ªO 139 msnm

EQP-CLL-513 26 18’10.0” S 58 48’54.0”W 57 msnmEQP-CLL-512 26 18’11.1” S 58 48’55.8”W 56 msnm

Global MalaiseProgram

Future plans (next 12 months)

• To continue working in collaboration with Bolivia to build its Biodiversity macroscope, overcoming its new regulations.

• To increase the production of COI products in CONICET’s Barcode Labs.

• To expand the Global Malaise Trap Program in protected areas in Argentina and Bolivia and set their sample processing pipelines.

• To help other Latin American countries in barcode projects – e.g. Peru, Dominican Republic, etc.

• To open a new call of projects for the iBOL Argentina Fund and to organize the Sixth LLABs training workshop for DNA barcoding.

Secured

Secured

Secured

Next trip to Bolivia postponed given current restrictions; additional support required to continue after 2013

Working on both aspects, additional funds required

Next biota?The Biodiversity of the Iguazú

National Park - Argentina

The Iguazú National Park (in Argentina) and the Iguaçu National Park (in Brazil) represent the southern tip of the Atlantic forest, and together with the preserves of Misiones Province are the largest and best preserved remains of this ecosystem.

The Atlantic forest, one of the richest and more endangered ecosystems of the World

Objectives1) To discover which species are within Iguazú National Park.2) To build a DNA barcoding database that will permit the identification of the

species of Iguazú National Park in a cost-effective and rapid manner.3) To describe the new species that will result from sampling the fauna of Iguazú

National Park.4) To provide a biodiversity data baseline for the Iguazú National Park that will be a

benchmark against which future biodiversity studies can be compared.