selective estrogen receptor α activation disrupts sex organ

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  • REPRODUCTIONRESEARCHSelective estrogen receptor a activation disrupts sex organdifferentiation and induces expression of vitellogenin II andvery low-density apolipoprotein II in Japanese quail embryos

    Anna Mattsson, Jan A Olsson and Bjorn Brunstrom

    Department of Environmental Toxicology, Centre for Reproductive Biology in Uppsala, Uppsala University,Norbyvagen 18A, SE-752 36 Uppsala, Sweden

    Correspondence should be addressed to B Brunstrom; Email: bjorn.brunstrom@ebc.uu.seAbstract

    The Japanese quail (Coturnix japonica) is a widely used model species for studying the roles of steroid hormones in avian sex

    differentiation. The aim of the present study was to elucidate the significance of estrogen receptors a and b (ERa and ERb) in normal

    sex differentiation of the reproductive organs in the Japanese quail and in xenoestrogen-induced disruption of reproductive organ

    differentiation. Real-time PCR indicated that ERa (ESR1) mRNA is expressed in both right and left gonads and Mullerian ducts (MDs) in

    both sexes during early morphological differentiation. ERb (ESR2) transcripts were also detected in gonads and MDs, but at very low

    levels. Both receptor subtypes were expressed in the liver and may therefore mediate the expression of estrogen-regulated egg-yolk

    proteins. Aromatase mRNA was expressed at much higher levels in female than male gonads as early as embryonic day 5, indicating early

    sex differences in estrogen synthesis. Treatment with the ERa-selective agonist propyl pyrazole triol showed that frequently reported

    xenoestrogen effects, such as ovotestis formation, abnormal MD development, and hepatic expression of egg-yolk proteins, were induced

    by selective activation of ERa. Taken together, our results suggest that activation of ERa is crucial for estrogen-dependent sex

    differentiation of the reproductive organs and that ERamediates xenoestrogen-induced toxicity during reproductive development in birds.

    Reproduction (2008) 136 175186Introduction

    The Japanese quail (Coturnix japonica) is a model speciesfor studying avian endocrinology and in ovo exposure ofquail embryos is often used to examine the endocrine-disrupting potential of chemicals in vivo. Estrogeniccompounds (xenoestrogens) may cause malformation ofreproductive organs (Berg et al. 1999), impaired sexualbehavior (Halldin et al. 1999), changes in sexuallydimorphic neural circuits (Panzica et al. 2002), andincreased expression of egg-yolk protein precursors inthe embryonic liver (Ichikawa et al. 2003). Severalenvironmental estrogens, including phytoestrogens suchas genistein and coumestrol and industrial chemicalssuch as nonylphenol and bisphenol A, have been shown tointeract differentially with the two nuclear estrogenreceptor (ER) subtypes, ERa and ERb (Jacob et al. 2001,Hanafy et al. 2005, Escande et al. 2006). The respectiveroles of ERa and ERb in mediating various xenoestrogen-induced effects, however, are poorly understood.

    Early in life, both male and female bird embryos havepairs of undifferentiated gonads and Mullerian ducts(MDs), which differentiate in a sex-dependent mannerduring gonadogenesis (Romanoff 1960). In females ofq 2008 Society for Reproduction and Fertility

    ISSN 14701626 (paper) 17417899 (online)most avian species, the reproductive organs developasymmetrically: the left gonad and MD develop into afunctional ovary and oviduct, respectively, whereas theright gonad and MD regress (Fig. 1A). In males, bothMDs regress and the gonads develop bilaterally intofunctional testes. Sexual differentiation of birds largelydepends on gonadal hormones produced by the embryo.Female bird embryos have higher plasma levels ofestradiol than male embryos (Woods & Brazzill 1981,Schumacher et al. 1988, Ottinger et al. 2001), andexperimental inhibition of the formation or action ofestrogen in the female embryo results in more or lesscomplete phenotypic sex reversal (testis formation, malesecondary sex characteristics, and lack of oviducaldevelopment; Scheib 1983, Elbrecht & Smith 1992,Abinawanto et al. 1997, Burke & Henry 1999, Vaillantet al. 2001). Conversely, exposure to xenoestrogensinduces formation of an ovary-like left testis (ovotestis) inmale embryos (Scheib 1983, Berg et al. 1999, 2001,Shibuya et al. 2005a, Razia et al. 2006), and embryonicexposure may also result in reduced semen production inadulthood (Blomqvist et al. 2006). Moreover, xenoestro-gens induce retention and abnormal development of theMDs in both sexes. In these cases, the MDs may becomeDOI: 10.1530/REP-08-0100

    Online version via www.reproduction-online.org

    http://dx.doi.org/10.1530/REP-08-0100

  • Figure 1 Ventral view of the genital system of 16-day-old male and female Japanese quail embryos (liver, heart, stomach, intestines, etc. removed).(A) General appearance of gonads and Mullerian ducts in control and xenoestrogen-exposed animals. (B) Examples of cystic Mullerian ducts inestrogen-exposed embryos. Cystic dilatations of the Mullerian ducts are formed in estrogen-treated embryos of both sexes; this is shown here in twofemales. 1, cloaca; 2, Wolffian duct; 3, mesonephros; 4, right testis; 5, left testis; 6, small right testis; 7, ovotestis; 8, retained Mullerian duct;9, vestigial right Mullerian duct; 10, vestigial right gonad; 11, left ovary; 12, left Mullerian duct; 13, cystic Mullerian duct.

    176 A Mattsson and othersgrossly malformed and cystic in both sexes and may evengo on to develop into malformed and functionallyimpaired oviducts (Nemirovsky & Narbaitz 1970,Holm et al. 2006).

    Increased expression of estrogen-inducible genescan be used as a marker for xenoestrogen exposure.In birds and other oviparous species, xenoestrogensinduce hepatic expression and increased serum concen-tration of the egg-yolk protein precursors, vitellogenin II(VTG II) and very low-density apolipoprotein II(apoVLDL II; Wiskocil et al. 1980, Sumpter & Jobling1995, Ichikawa et al. 2003, Marin & Matozzo 2004,Shibuya et al. 2005b).

    One way to elucidate the respective contributions ofERa and ERb to physiological functions and xenoestro-gen-induced endocrine disruption is to study the effectsof subtype-selective activation and inactivation. Theagonist propyl pyrazole triol (PPT) and the antagonistmethyl-piperidino-pyrazole (MPP) are well known andhighly selective ERa ligands (Stauffer et al. 2000, Sunet al. 2002). PPT has 50% lower affinity for human ERa(hERa) than estradiol, and about 400-fold higher affinityfor hERa than hERb (Stauffer et al. 2000). In humanendometrial cancer (HEC-1) cells, PPT regulates genetranscription through hERa from different reporterconstructs that contain various estrogen-responsivepromoter elements, but has no activity through hERb(Stauffer et al. 2000, Harrington et al. 2003). In HEC-1cells, MPP displays full antagonism of ERa-mediatedestradiol stimulation (Harrington et al. 2003).

    The aim of the present study was to explore the roleof ERa in normal and disrupted differentiation of thereproductive organs in Japanese quail embryos, and inxenoestrogen-induced hepatic expression of egg-yolkprotein mRNA. We examined the expression of ERa(ESR1) and ERb (ESR2) mRNAs in xenoestrogen targetorgans, i.e., gonads, MDs, and liver. To study the potentialfor gonadal estrogen synthesis, we examined theexpression of P450 aromatase (P450arom) mRNA in theReproduction (2008) 136 175186developing gonads. We then exposed quail embryos tothe ERa-selective agonist PPT and the ERa-selectiveantagonist MPP in order to investigate whether (a) ERacan mediate xenoestrogen-induced disruption of sexorgan differentiation, (b) ERa inactivation can disturbnormal female reproductive development, and (c) ERacan mediate xenoestrogen-induced expression of VTG IIand apoVLDL II in the embryonic liver. The embryos wereexposed to PPT, MPP, or a combination of both fromembryonic day 3 (before morphological sex differen-tiation starts) until embryonic day 16, when the effects ongonads, MDs, and hepatic gene expression wereassessed. As a positive control, we used ethinylestradiol(EE2), a synthetic estrogen that binds both quail ERsubtypes (Hanafy et al. 2004, 2005) and acts as an agoniston both hERa and hERb (Barkhem et al. 1998). EE2 iscommonly used as a positive control for estrogenicactivity and has proven to be effective in previous studiesusing the quail model (Berg et al. 1999, 2001).Results

    Morphological differentiation of gonads and MDs

    Morphological differentiation of the gonads was firstrecognized at day 7 (stage 33) and was seen as a slightasymmetry in the size of the gonads in four out of fivedissected females. At the histological level, the sexdifferences may exist from day 5.5 (Scheib et al. 1985).On day 9 (stage 37), the right gonad of the female wasalmost completely regressed.

    The sex differences in MD morphology were firstobserved on day 8; the MDs were thicker in females thanin males. On day 9, the MDs in all examined males werepartly regressed and very thin. These were therefore notsampled for gene expression analysis. In five out of theeight females, the right MD had started to regress in acraniocaudal direction on day 9.www.reproduction-online.org

  • ERa-mediated effects in quail embryos 177Expression of P450arom , ERa, and ERb mRNA in gonads

    The reaction efficiencies were 100% for b-actin,P450arom, and ERb and 90% for ERa. The negativecontrols showed no contaminating genomic DNAamplified in the real-time PCR. The melting curves ofthe PCR products also confirmed that no unspecificsequences were amplified.

    Total P450arom expression in female gonads increasedbetween day 5 and day 9 (stage 2837), whereas theexpression in males was low throughout the entireperiod (Fig. 2A). On embryonic days 7 and 8 (stages 33and 35), the P450arom mRNA expression in both left andright go

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