Project leader: Daniela GoltsmanProject leader: Daniela Goltsman

Jose Villanueva, Victoria Partida,Jose Villanueva, Victoria Partida,

Katherine CantareroKatherine Cantarero

Microbial DiversityMicrobial Diversity

Table Of Contents. . .Table Of Contents. . .

• Introduction

• Inoculation

• Isolation

• FISH

• Results

• Conclusion

BackgroundBackground

• Why are microorganisms important?

• How are microorganisms studied today?– Aseptic technique – Dyes

• What was the goal of our experiment? – To study microbial diversity around our

environment

Diversity of lifeDiversity of life

Sampling SitesSampling Sites

• Our samples:– Jose’s bathroom– UC Berkeley’s Strawberry Creek – Foothill Rec. Room Keyboard– Katherine’s Dorm

• Why did we choose these samples?

Which location do YOU think is the diverse or “dirtiest”“dirtiest” ?

What is inoculation?What is inoculation?

• The introduction of microorganisms into any media.

• For us this process took about three days to complete.

• We incubated at 37 00 C

Jose’s BathroomJose’s Bathroom

Katherine’s dormKatherine’s dorm

Foothill Rec. room keyboardFoothill Rec. room keyboard

UC Berkeley’s Strawberry CreekUC Berkeley’s Strawberry Creek

IsolationIsolation

• Taking one colony or “population” of one type of microorganism and making those populations reproduce and grow.

Isolate from Jose’s bathroomIsolate from Jose’s bathroom

Isolate from rec. room keyboardIsolate from rec. room keyboard

UC Berkeley’s Strawberry CreekUC Berkeley’s Strawberry Creek

What is FISH?What is FISH?

• FISH: Fluorescent In Situ Hybridization.

• This is a powerful technique that can be used to evaluate and analyze the presence of organisms in their community, their phylogeny, morphology, and number by targeting the 16S rRNA.

FISH probesFISH probes

• What is a probe?– Short sequence of DNA that hybridizes

(binds) to a segment of the 16S rRNA. – Each probe carries a different color dye.

• Our dyes:– 4 different probes 3 different colors– Blue- Gama proteobacteria & Eukaryotic cells– Green- Universal bacteria– Red- Beta proteobacteria

Sample 1 LAPTOP. Green: bacteria, red: Sample 1 LAPTOP. Green: bacteria, red: beta-proteobacteriabeta-proteobacteria.

Sample 2: STRAWBERRY CREEK Green: universal Sample 2: STRAWBERRY CREEK Green: universal bacteria, red: beta-proteobacteria, soft blue: gama-bacteria, red: beta-proteobacteria, soft blue: gama-

proteobacteria, bright blue: eukaryotes.proteobacteria, bright blue: eukaryotes.

Sample 3:KATHERINE’S ROOM Red: Sample 3:KATHERINE’S ROOM Red: beta-proteobacteria, blue: eukaryotes.beta-proteobacteria, blue: eukaryotes.

Sample 4. Green: universal bacteria, Sample 4. Green: universal bacteria, blue: eukaryotesblue: eukaryotes

ConclusionConclusion

In conclusion, we learned that:

– Microorganisms are EVERYWHEREEVERYWHERE!– There are many kinds of bacteria that take on

many shapes and sizes.– Keyboards and bathrooms are the most

diverse–

Thank you!Thank you!