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CURSO TEORICO-PRACTICO: ESTANDARIZACIÓN DEL ANÁLISIS BÁSICO DEL SEMEN EN LOS LABORATORIOS CLINICOS 27-28 DE FEBRERO DEL 2014 CHILPANCINGO, GRO. 1 www.analisisdesemen.com.mx COLEGIO GUERRERENSE DE QUÍMICOS CLÍNICOS A.C. PROGRAMA NACIONAL PARA LA ESTANDARIZACIÓN DEL ANÁLISIS BÁSICO DE SEMEN (PRO-SEMEN)

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CURSO TEORICO-PRACTICO:ESTANDARIZACIN DEL ANLISIS BSICO DEL SEMEN EN LOS LABORATORIOS CLINICOS27-28 DE FEBRERO DEL 2014 CHILPANCINGO, GRO1!!!"#"$%&%&'(&()(#*+)),COLEGIO GUERRERENSE DE QUMICOS CLNICOSA.C.PROGRAMA NACIONAL PARA LA ESTANDARIZACINDEL ANLISIS BSICO DE SEMEN (PRO-SEMEN)2 !!!"#"$%&%&'(&()(#*+)),E$ +-.(/%0+ '($ *12&+ (& 31( 1&/(' "42(#'" ($A#5$%&%&B5&%*+'(S()(#6$+ (&/"-$(7*" (# &1 $"-+2"/+2%+ E$*12&+(&'(&"22+$$"'+'("*1(2'+"$"& #+2)"&(&/"-$(*%'"&4+2$"O28"#%7"*%9# M1#'%"$ '( $" S"$1' :OMS;OB=ETI>O3 !!!"#"$%&%&'(&()(#*+)),IMPORTANCIA DEL ANALISIS BASICO DEL SEMENE$"#5$%&%&'(&()(##+&42+4+2*%+#" %#?+2)"*%9#(&(#*%"$&+-2(($(&/"/1& *$@#%*+ '($ %#'%0%'1+T+'""$/(2"*%9#'($"?1#*%9#(#'+*2%#"+ (,+*2%#"'($/(&/@*1$+&(2(A(."(#($ "#5$%&%&'(&()(#,6"&("(#&1&"&4(*/+& )+2?+$98%*+& 6B+ ?1#*%+#"$(&4 !!!"#"$%&%&'(&()(#*+)),IMPORTANCIA DEL ANALISIS BASICO DEL SEMEN1CD '( $"& P"2(."& &+#I#?E2/%$(&F"*/+2 M"&*1$%#+40DF"*/+2 M"&*1$%#+FF"*/+2 F()(#%#+20D5 !!!"#"$%&%&'(&()(#*+)),CAUSAS DE LA INFERTILIDAD MASCULINAOATD%&?1#*%9# &(,1"$ 6B+ (6"*1$"/+2%"C"1&" %#)1#+$98%*"A#+2)"$%'"'(& "%&$"'"& '($ 4$"&)" &()%#"$C"1&"& %"/2+8E#%*"&C"1&"& &%&/E)%*"&A#+2)"$%'"'(& *+#8E#%/"&>"2%*+*($(D"G+ /(&/%*1$"2 "'31%2%'+ C"1&"& (#'+*2%#"&A7++&4(2)%" +-&/21*/%0" 6 #+ +-&/21*/%0"C"1&" #+ '()+&/2"-$(6 !!!"#"$%&%&'(&()(#*+)),Necrozoo!er"#$ o A%e&ozoo!er"#$ '$($ ((o )$%o &o co&c*er)$&)Necrozoo!er"#$ o A%e&ozoo!er"#$ '$($ ((o )$%o &o co&c*er)$&)P$%o(o+,$ o #&%er-e&c#o&eP$%o(o+,$ o #&%er-e&c#o&eReco+#)$ #&$)ec*$)$ (coitus interruptus)Reco+#)$ #&$)ec*$)$ (coitus interruptus)Po#.(eCo&%$"#&$c#/&Po#.(eCo&%$"#&$c#/&A.%#&e&c#$ Pro(o&+$)$A.%#&e&c#$ Pro(o&+$)$Tr$&!or%e I&$)ec*$)oTr$&!or%e I&$)ec*$)oRe%r$o e& ($ E&%re+$ )e ($ M*e%r$Re%r$o e& ($ E&%re+$ )e ($ M*e%r$E0$c*($c#/&'r$cc#o&$)$E0$c*($c#/&'r$cc#o&$)$HC1"#'+ R(4(/%2 1# A#5$%&%& '( S()(#I7 !!!"#"$%&%&'(&()(#*+)),AGENDA DEL CURSOSABADO:PARTE TEORICA;INTRODUCCION: FISIOLOGIA DEL APARATO REPRODUCTOR MASCULINOMODULO I: RECOLECCION DE LAS MUESTRAS DE SEMENMODULO II:ANALISIS MACROSCOPICO DEL SEMENMODULO III:E>ALUACION DE LA MO>ILIDAD ESPERMATICAMODULO I>:E>ALUACION DE LA >IABILIDAD ESPERMATICAMODULO >: E>ALUACION DE LA CONCENTRACION ESPERMATICAMODULO >I: E>ALUACION DE LA MORFOLOGIA ESPERMATICAMODULO >II: E>ALUACION DE OTROS TIPOS CELULARES PRESENTES EN EL SEMENMODULO >III: CONTROL DE CALIDAD EN EL ANALISIS DE SEMENINTERPRETACION JUIMICA DEL ANALISIS DE SEMENDOMINGO :PARTEPRACTICA;8 !!!"#"$%&%&'(&()(#*+)),INTRODUCCION70D20DC D$1-2%*"*%9#C D9 !!!"#"$%&%&'(&()(#*+)),VALORES NORMALES DEL SEMEN HUMANOPARAMETRO >ALOR DE REFERENCIA>+$1)(# 20 )L + )5&4H 72 + )5&C+#*(#/2"*%9# E&4(2)5/%*" 20 )%$$+#(&B)L + )5&NK)(2+ T+/"$ '( E&4(2)"/+7+%'(&40 )%$$+#(&BE6"*1$"'+ + )5&M+0%$%'"' C0D + )5& *+# )+0%$%'"' A 6 B2CD + )5& *+# )+0%$%'"'AM+2?+$+8@" C+#&(#&+ (# P2+*(&+>%"-%$%'"' C0D '( 0%0+& + )5&L(1*+*%/+& M(#+& '( 1 )%$$9#B)LWHO. 1999. Laboratory manual for the examnaton of human !emen an" !#erm$%er&%al mu%u! ntera%ton. 'ourth E"ton( )ambr"*e Un&er!ty +re!!.10111.$&$(##)ee"e&.co"."2ELACULADOEMAMENMACROSCOPICO>ISCOSIDADASPECTO >OLUMEN4H>ITALIDADEMAMENMICROSCOPICOCONCENTRACIONMOTILIDADAGRUTINACION L AGREGACIONOTRAS CELULASMORFOLOGIA ESPERMATICAANTICUERPOSANTI-ESPERMATOZOIDESANALISIS DEL SEMENLICUEFACION11 !!!"#"$%&%&'(&()(#*+)),VAR,A-LES DEL SEMENN+2)+7++&4(2)%" E6"*1$"'+ *+# 0"$+2(& #+2)"$(&H%4+&4(2)%" >+$1)(# &()%#"$ '%&)%#1%'+O$%8+7++&4(2)%" C+#*(#/2"*%9# '( (&4(2)"/+7+%'(& )(#+2 "$ 0"$+2 '( 2(?(2(#*%"A&/(#+7++&4(2)%" M+0%$%'"' %#?(2%+2 "$ 0"$+2 '( 2(?(2(#*%"T(2"/+7++&4(2)%" M+2?+$+8@" %#?(2%+2 "$ 0"$+2 '( 2(?(2(#*%"O$%8+"&/(#+/(2"/+7++&-4(2)%"A$/(2"*%9# '( $"& /2(& 0"2%"-$(&N(*2+7++&4(2)%" E&4(2)"/+7+%'(& )1(2/+&A7++&4(2)%" A1&(#*%" '( (&4(2)"/+7+%'(& (# ($ (6"*1$"'+A&4(2)%" N+ N"6 (6"*1$"'+12 !!!"#"$%&%&'(&()(#*+)),MODULO IRECOLECCION DE LAS MUESTRAS DE SEMENAREAS ESPECIALESLIMPIEZA DE LAS AREAS DEL LABORATORIO13 !!!"#"$%&%&'(&()(#*+)),,NS.RU)),ONES AL +A),EN.E-A-&/%#(#*%" S(,1"$ '( 2-7 '@"&-A&(+ *+# "81" 6 ."-9# '( 8(#%/"$(&-T+)" '( )1(&/2" (# ($ $"-+2"/+2%+-C+$(**%9# '( )1(&/2" 4+2 )"&/12-"*%9#-A#5$%&%& '( 2 )1(&/2"&-N+ &( 4(2)%/( ($ *+%/+ %#/(221)4%'+-D( 42(?(2(#*%" *+$(*/"2 $" )1(&/2" (# ($ $"-+2"/+2%+-N+ &( '(-( 4(2'(2 #%#81#" ?2"**%9# '($ (6"*1$"'+14 !!!"#"$%&%&'(&()(#*+)),MANE/O DE LAS MUES.RAS$Me""a! "e +rote%%0n.$Et1ueta"o "e la Mue!tra.$.ra!la"o "e la mue!tra a la ,n%uba"ora a 234)$+rote%%0n "e la! tem#eratura! extrema!15 !!!"#"$%&%&'(&()(#*+)),MODULO IIANALISIS MACROSCOPICO DEL SEMENLICUEFACCIONP2+*(&+(#($*1"$$")1(&/2"4"&"'(1#(&/"'+ N%4(20%&*+&+ " 1# (&/"'+ $%31%'+ :)"#(."-$(;PARAMETRO >ALOR DE REFERENCIALICUEFACCION 10 O P0 MINF+&?"/+ '( (&4(2)%#"F+&?"/"&" 5*%'"Q L%*1"# ($ &()(#RETARDADA MAS DE P0 MINUTOSDISFUNCION DE PROSTATA G25#1$+& G($"/%#+&+&-L%*1(?"**%9# I#*+)4$(/"16 !!!"#"$%&%&'(&()(#*+)),>OLUMENPARAMETRO >ALOR DE REFERENCIA>+$1)(# 20 )LH%4+&4(2)%" >+$1)(# &()%#"$ '%&)%#1%'+A&4(2)%" N+ N"6 (6"*1$"'+MENOS DE 2 MLPERDIO FRACCIONELACULACION RETROGRADA17 !!!"#"$%&%&'(&()(#*+)),ASPECTOPARAMETRO VALOR DE REFERENCIAASPECTO GRIS OPALECENTECAFR Q ERITROCITOS >ERDE Q BACTERIASAMARILLO Q ICTERICIA18 !!!"#"$%&%&'(&()(#*+)),>ISCOSIDADPARAMETRO >ALOR DE REFERENCIA>ISCOSIDADNORMAL 20 :GOTA A GOTA;>ISCODIDAD AUMENTADA S0 :FILAMENTOS;>ISCOSIDAD DISMINUIDA T20 :GOTA SIN CONSISTENCIA;DISMINUIDA:CAE COMO CHORRO;FALLA DE >ESICULAS SEMINALES19 !!!"#"$%&%&'(&()(#*+)),4HPARAMETRO >ALOR DE REFERENCIA4H 72 O 80MENOR DE 72LESION DE >ESICULAS SEMINALESMALOR DE 8LESION DE PROSTATA20 !!!"#"$%&%&'(&()(#*+)),MODULO IIIMO>ILIDAD ESPERMATICAL+&(&4(2)"/+7+%'(&/%(#(#31(/(#(2)+0%$%'"' 4"2")%82"2N"*%"($)+*+*(20%*"$(#($/2"*/+ 8(#%/"$?()(#%#+,($$+&4(#(/2"#($cumulus oophorus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istribucin de Poisson :#K)(2+ '( (&4(2)"/+7+%'(&B$(1*+*%/+&; Distribucin Binomial :D )+/%$%'"', D )+2?+$+8@", D 0%/"$%'"'; 23 !!!"#"$%&%&'(&()(#*+)),M+0%$%'"' (&4(2)5/%*": 42+?1#'%'"' '( $" *5)"2"C+#/"2 " TW ")-%(#/( + S7XC(# *5)"2"& *+# 1#" 42+?1#'%'"' '( Y20 Z), 4"2" 4(2)%/%2 ($ )+0%)%(#/+ $%-2(L" 42+?1#'%'"' :D,Z); (& ($ 0+$1)(# :>, Z$Q))[; '%0%'%'+ 4+2 ($ 52(" :A, ))\; C+#/"2 2 , 200 (&4(2)"/+7+%'(& :&% (& 4+&%-$(; (# "$ )(#+& C *")4+& '%?(2(#/(&U&"2 1# +*1$"2 2(/%*1$"'+ 4"2" $%)%/"2 ($ 52(" (,")%#"'" (0%/"#'+ $+& $%)%/(& '($ *1-2(+-.(/+&"; -;> 5 mm24 !!!"#"$%&%&'(&()(#*+)),M+0%$%'"' (&4(2)5/%*": *$"&%]*"*%9#S( *+#&%'(2"'" *+)+:OMS^4G2"'+ AQ 42+82(&%0" $%#("$ 254%'" :_2C Z)B& " S7XC;G2"'+ BQ 42+82(&%0" #+ $%#("$ $(#/"G2"'+ CQ#+ 42+82(&%0" G2"'+ DQ %#)+0%$(&*"-(7"& *+$"25 !!!"#"$%&%&'(&()(#*+)),CLASIFICACION DE LA MO>ILIDAD26 !!!"#"$%&%&'(&()(#*+)),CLASIFICACION DE LA MO>ILIDAD1C0 )B& C0 )B& S0 )B& 20 )B& 7 )B&7C )B& 40 )B& 2C )B& 1C )B& 4 )B&Yeung et al., 199727 !!!"#"$%&%&'(&()(#*+)),VER VIDEO TAPE DE MOVILIDAD- C+#/"2 )@#%)+ 200 (&4(2)"/+7+%'(& (# 40,-C+#/"2 42%)(2+(# ($ )%&)+ *")4+,82"'+ A 6 B4+&/(2%+2)(#/( C 6 D28 !!!"#"$%&%&'(&()(#*+)),E=EMPLO:AQ SSB210 M 100 Q 1C7DQ 1PDBQ `SB210 M 100 Q 442DQ44DCQ 2PB210 M 100 Q 12SDQ12DDQ C8B210 M 100 Q 27PDQ28DTOTALQ100D29 !!!"#"$%&%&'(&()(#*+)),PARAMETRO >ALOR DE REFERENCIAMovili! 50" o m!# $o% movili! 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