Test Information

MethodComponents of this Pemphigus Panel are epithelial cell surface (CS) IgG antibodies by indirect immunofluorescence (IFA) on intact human skin and monkey esophagus substrates, and desmoglein 1 and desmoglein 3 IgG antibodies by ELISAs. See Compliance Statement A: www.aruplab.com/CS.

IgG epithelial cell surface antibodies – serum is progressively diluted in calcium-containing buffer beginning at 1:10 in three two-fold dilutions for screening. Each dilution is layered on sections of intact normal human skin and monkey esophagus substrates, and stained with fluorescein-conjugated anti-IgG using Analyte Specific Reagents (ASRs). Positive antibody-containing serum is retested and titered by two-fold dilution to the end point of antibody detection.

Desmoglein 1 and desmoglein 3 IgG serum antibodies – antibody levels determined by FDA-approved enzyme linked immunosorbent assays (ELISAs). (DSG 1 & DSG 3 ELISA Test System, MBL BION).

UseAid in the diagnosis of major types of pemphigus and to monitor disease activity and therapeutic response.

Use Pemphigus Panel or the IgG desmoglein 1 and desmoglein 3 assay to monitor pemphigus vulgaris and pemphigus foliaceus disease activity and therapeutic response.

ConsiderationsRecommend use of this panel along with Pemphigoid Panel and Endomysial IgA antibody tests to initially diagnose and distinguish various immunobullous disorders in patients suspected or known to have any type of immunobullous disease.

Recommend perilesional skin biopsy in Michel's or Zeus' medium for cutaneous direct immunofluorescence.

Repeat Pemphigus Panel for indeterminate results and/or continuing clinical consideration of pemphigus vulgaris or pemphigus foliaceus, persistence, and/or worsening disease activity.

Use separate IgA Pemphigus Antibody test to monitor disease activity in IgA pemphigus. Use Paraneoplastic Pemphigus screen for patients who may have paraneoplastic pemphigus.

For testing algorithm and additional information about immunobullous skin diseases, refer to: arupconsult.com/Topics/ImmunobullousSkinDz.

Patient Report

Patient's immunodermatology report from the University of Utah continues on following pages.

Patient: DOB: Age: Gender: Patient Identifiers:

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ARUP Test Code: 0090650

Collection Date: 11/29/2016Received in lab: 12/01/2016Completion Date: 12/06/2016

Pemphigus Antibody Panel - Epithelial Cell Surface Antibodies and Desmoglein 1 and Desmoglein 3

Antibodies, IgG

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Patient: ARUP Accession: 16-334-136007