native larvivorous fish diversity as a biological control ...back.nimr.org.in/assets/545034.pdf ·...

J Vector Borne Dis 55, March 2018, pp. 34–41

INTRODUCTION

Mosquitoes are small insects, which transmit various diseases, like malaria, filariasis, Japanese encephalitis, dengue, chikungunya, and Zika, etc1–2. These diseases have become serious public health problems in the tropi-cal and subtropical regions of the world3. Multifarious ap-proaches have been adopted for controlling the mosquito populations to reduce the incidence of vector-borne dis-eases, which includes chemical, physical and biological measures. However, use of chemical insecticides against malaria vectors, which started about five decades ago, has resulted in insecticide resistance in mosquitoes. In-

secticides contaminate the food chain and possess seri-ous health hazards, besides, evolving physiological resis-tances to the vector species resulting in failure of vector control methods and harmful effects on beneficial non-target animals4–5. Also, the chemical and physical control measures are more expensive and time-consuming for regular entomological surveillance of mosquito breed-ing sites. These issues are the most important reasons for changing the national approaches to malaria control campaigns with integrated strategies including biological control methods. The biological control strategy against mosquito vectors is safe, friendly, and cost-effective in all type of habitats, i.e. natural and artificial6. The bio-logical control of mosquitoes involves introduction of their natural enemies likes parasites, disease organisms

Native larvivorous fish diversity as a biological control agent against mosquito larvae in an endemic malarious region of Ranchi district in Jharkhand, India

Manoj Kumar Das*, M. Rajesh Kumar Rao* & A.K. Kulsreshtha

ICMR–National Institute of Malaria Research, Field Unit, Ranchi, Jharkhand, India

ABSTRACT

Background & objectives: Mosquitoes are responsible for transmitting several diseases, including malaria, dengue, chikungunya, filariasis, and yellow fever, etc. Release of larvivorous fishes is one of the cheapest method of vec-tor management approach, with long suppression of mosquito population. The present study identifies the native larvivorous fishes and evaluates their potential larvivoracity for biological control of mosquito larvae in an endemic malarious region.Methods: During the year 2012–13, an ecological descriptive study was carried out in diverse aquatic habitats of fish species found in different areas of Ranchi district, in Jharkhand state of India. Fishes were captured using fishing nets, and identified and classified according to the available keys. Their larvivorous potential was graded according to their feeding potential. Data on current conservation status as well as their abundance were also recorded and analysed.Results: In total, 30 larvivorous fish species belonging to seven orders, 10 families and 21 genera were identified. Order Cypriniformes and the family Cyprinidae were the most ascendant group constituting 66.7 and 60%, respec-tively. The grading assessment of larvivorous potential for different fish species revealed that, Colisa fasciatus pos-sess maximum larvivoracity (+ + + + +). According to the conservation, assessment and management plan (CAMP, 1998), 60% species were at lower risk near threat (LRnt), while 86.7% species were at least concerned (LC) as per the IUCN, 2017 categorisation. All fish species preferred to inhabit in freshwater. Maximum species occurrence was found in the river (63.3%). Only 30% species were bottom feeders (BF). Interpretation & conclusion: The larvivoracity and habitat distribution analysis indicated that C. fasciatus, Oreo-chromis mossambica, Esomus danricus, Oryzias melastigma, Puntius sophore, P. ticto, Rasbora daniconius, R. elegans, Aplocheilus panchax, and Danio (B) rerio possess high-level larvivorous potentiality in nature and are recommended for malaria control in the study area. There is an increasing pressure on the fish fauna, of facing several threats, such as fishing, human interference, loss of habitat, overexploitation, pollution, siltation, trade, and diseases. Therefore, periodic survey and monitoring of fish biodiversity, demarcation of breeding sites, field level research study on the efficacy of these fishes, and public awareness on establishment of larvivorous fish ponds should be adopted as a part of the vector management approach in the endemic malarious region of Ranchi district in Jharkhand, India.

Key words Biological control; larvivoracity; larvivorous fish; mosquito larvae; Ranchi

*Authors made equal contribution.

35Das et al: Larvivorous fish diversity as a biological control agent against mosquito larvae

and predatory animals into the environment7. Continuous usage of biological control agents has showed minimal side-effects on humans, domestic creatures and on wild-life. Among all the bio–control agents, use of larvivorous fishes are the most efficient method in mosquito control at their immature stages all over the world, since the early-1900s8–9. Various indigenous fish species have been also used for mosquito control which provides dual benefits, e.g. reducing the mosquito populations and supplement-ing the economics of fish culture10.

In India, larvivorous fishes were experimentally used for the first time in 1904 for the control of malaria vectors in Mumbai City11. Soon after that usage of larvivorous fish became a common practice for malaria vector control in India; however, during the 2000s there was decline in the usage of this strategy12.

In recent years, a few larvivorous fish fauna surveys were undertaken from various parts of the India to scale-up the biological control of mosquitoes. In 2007, Ghosh and Dash12 reported that 315 fish species under seven gen-era having larvivorous nature could be used as larvivorous fish in malaria control. In 2014, Ramarao13 reported 58 species of larvivorous fish from the lower Manair Dam at Karimnagar, Andhra Pradesh. In 2015, Rao et al14 report-ed 22 species of larvivorous fish (eight orders) from Lake Kolleru, Andhra Pradesh. Similarly, in 2016, Krishna et al15 reported 29 species of larvivorous fish from six or-ders, 14 families and 20 genera from Lake Kolleru.

Use of larvivorous fish as part of the vector manage-ment approach remains under-exploited in India or else-where in recent times. There is an urgent need to strength-en the strategy, and to provide appropriate training, for the use of larvivorous fishes to scale-up the control of mosquitoes. Till date, systematic studies and surveys on the larvivorous fish fauna of the Jharkhand state have not been undertaken16. Keeping this in view, this study aimed to find native larvivorous fish species and evaluate their potential activity for biological control of mosquito larvae in the malarious area of the Ranchi district of Jharkhand, India.

MATERIAL & METHODS

Study areaRanchi is situated in the central province of the

Jharkhand state in India, and lies between 23° 35' N lati-tude and 85° 33' E longitude. The geographical area of the district is 177.19 km2. Its elevation from sea level is 2140 ft. Ranchi is in the southern part of the Chota Nag-pur plateau, which is the eastern section of the Deccan plateau. The climatic condition of Ranchi is moderate due

to the hills and dense deciduous forest17. The main sam-pling sites and the rivers (Subarnarekha, South Koel and its tributaries) flowing through Ranchi district are shown in Fig. 1.

Study period This ecological descriptive study was carried out in

diverse aquatic habitats of fish species found in different areas of Ranchi district, during the year 2012 and 13.

Methodology Fish fauna survey and collection was carried out in

different water bodies including rivers, swamps, ponds, streams, water canals, tanks, and rice fields using the fish-ing nets and the local traditional fishing contraptions/apparatus prepared by bamboo and thread, called lajal (pushing net), jakoi (basket trap), chepa (valve trap), and khoka during all the seasons. Photographs of the fish spe-cies were taken for appropriate identification before pres-ervation. The bionomics of the habitats, where fishes were captured, was recorded in the form of ecological notes. Specimens were preserved in formalin concentrates (so-lution) according to their size. Smaller size specimens were preserved in 8% formalin and medium sized ones prior to the fixation, were incised longitudinally in the abdomen. Bigger size specimens were fixed by injecting 10% formalin into the muscles and abdomen area. The specimens were kept in a glass container with proper lev-eling and tail upward direction to avoid damage to the caudal fin for proper identification. Identification and classification of the fishes were carried out using the keys

Fig. 1: Map of Ranchi district showing sites of fish fauna survey.

J Vector Borne Dis 55, March 201836

for fishes developed by Jayaram18, and Talwar and Jhin-gran19. The evaluation of larvivorous potential of fish spe-cies was carried out in the laboratory for seven consecu-tive days against mosquito larvae. The fishes were graded according to their feeding potential (Consumption of larvae/day: 100 or more = + + + + +; 75 – 99 = + + + +; 50 – 74 = + + +; 25 – 49 = + +; 1 – 24 = +). Data on cur-rent conservation status were also collected from the re-port on the Conservation, Assessment and Management Plan (CAMP) workshop on freshwater fishes of India, and IUCN Red List Category of Threatened Species20-21. Data on the abundance of different fish species, threats faced by the fish fauna, and their economic importance were recorded from direct observations (primary sources) and literature and reports (secondary sources)9-15.

Ethical statementNo ethical issues are involved with this study.

Statistical analysisThe Microsoft’s Excel-2007 was used for statistical

analysis of the data generated.

RESULTS

The grading assessment of larvivorous potential for different fish species revealed that, it was highest for Coli-sa fasciatus (+ + + + +), followed by Oreochromis mos-sambica, Esomus danricus, Oryzias melastigma, Puntius sophore, P. ticto, Rasbora daniconius, R. elegans, Aplo-cheilus panchax, and Danio (B) rerio (+ + + +). Least potential was found in L. calbasu, L. rohita, Nemacheilus savona and Nemacheilus aureus (+). Laboratory evalua-tion of these 30 fish species indicated that only 10 fresh-water fish species, namely C. fasciatus, O. mossambica, O. melastigma, D. rerio, E. danricus, P. ticto, P. sophore, R. daniconius, R. elegans, and Aplocheilus panchax pos-sessed good feeding potential towards mosquito larvae (Fig. 2).

The abundance and larvivorous potential for different fish species is presented in Table 1. The result revealed that 10% (3) species —D. rerio, E. danricus, and O. moss-ambica were most abundant; 30% (9) species—Cyprinus carpio, N. aureus, P. sophore, P. ticto, R. daniconius, C. fasciatus, O. melastigma, R. elegans, and Mystus bleekeri were moderately abundant; 40% (12) species—Channa punctatus, Amblypharyngodon mola, Chela atpar, C. laubuca, Cirrhinus mrigala, C. reba, Labeo calbasu, L. rohita, P. phutunio, A. panchax, Anabas testudineus, and C. gachua were less abundant; and 20% (6) spe-cies—L. bata, N. savona, Oxygaster bacaila, P. sarana,

Notopterus notopterus, and C. lalia were rarely available in the study area.

The feeding habits of the studied fish species indicated that 30% species were bottom feeders (BF), 23.3% were both surface feeders (SF) and omnivore (O), 16.7% were bottom columnar feeder (BCF), 10% were both carnivore (C) and predatory (P) in nature; and 6.7% species were both herbivorous column feeder (HCF) and sub-surface feeder (SSF) (Fig. 3).

The diversity of fish species inhabited is presented in Table 1 and Fig. 4. The result revealed that 63.3% spe-cies occurrences were found in the rivers (R), 53.3% in the ponds (P), 33.3% in streams (S), 23.3% in tanks (T), 13.3% in rice fields (RF), and 10% in water canals (WC). All 30 larvivorous fishes recorded preferred to inhabit in freshwater; however, 10 species were recorded from both freshwater and brackish water regions.

The conservation status, commercial importance, and threats to the larvivorous fish species caught from differ-ent water bodies in the study area are presented in Table 2.

Rasbora elegans Aplocheilus panchax

Oryzias melastigma Danio (B) rerio

Fig. 2: High-level larvivorous potentiality recorded in 10 fish species found in Ranchi district of Jharkhand, India.

Colisa fasciatus Oreochromis mossambica

Esomus danricus Puntius ticto

Puntius sophore Rasbora daniconius

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Fig. 3: Percent occurrence of the fish species in accordance to their feeding habits; BCF—Bottom columnar feeder; P—Predatory fish; C—Carnivore; SSF—Subsurface feeder; BF—Bottom feeder; HCF—Herbivorous column feeder; SF—Surface feeder; SCBF—Surface column bottom feeder; O—Omnivore; SCF—Surface columnar feeder; CF—Columnar feeder.

Fig. 4: Percent occurrence of the fish species in accordance to their habitats; R—Rivers; P—Ponds; S—Streams; T—Tanks; RF—Rice fields; WC—Water canals; SW—Swamps.

The result revealed the presence of 30 larvivorous fish species belonging to seven orders, 10 families, and 21 genera. Out of the total species, order Cypriniformes was the most ascendant group constituting 66.7%, followed by Perciformes with 16.7%. Order Cypriniformes com-prised of two families with 20 species of fish, followed by Perciformes which included four families with five fish species ; and Beloniformes, Channieformes, Cyprin-odontiformes, Osteoglossiformes, and Siluriformes, each (family) with one fish species. Out of 10 families record-ed, Cyprinidae with 18 species contributed 60% to the total species followed by Nemacheilidae, Channidae, and Osphronemidae each with two species contributing about 6.7%, and Adrianichthyidae, Aplocheilidae, Notopteri-dae, Cichlidae, Anabantidae, and Bagridae each with one fish species contributing about 3.37%.

According to the report on the CAMP workshop on freshwater fishes of India, 18 (60%) species were at lower risk near threatened (LRnt), 5 (16.7%) species were at lower risk least concern (LRlc), 5 (16.7%) species were vulnerable (VU) and for 2 (6.7%) species data was defi-

cient (DD). As per the IUCN (2017) Red List Category of Threatened Species, 26 (86.7%) species were at least concern (LC), 1 (3.3%) at near threatened (NT), 1 (3.3%) is not evaluated (NE) and for one species (3.3%) data is deficient (DD).

Figure 5 shows the use and trade of the captured/iden-tified fish species. Of these, 19 (63.3%) fish species were used as food fish (F), 18 (60%) were cultivable (CL), and 16 (53.3%) were used as an ornamental fish (OF). Medici-nal value (MD) was found in 2 (6.7%) of the fish species.

Figure 6 shows different types of threats to fish popu-lation along with their habitats. Major threat (73.3%) ob-served was trade (T), followed by human interference (Hi) (50%), siltation (Sn) (20%), loss of habitat (Lh) (30%) and disease (D) (6.7%).

DISCUSSION

Monitoring the diversity of larvivorous fish fauna for their evaluation as a part of mosquito vector management is important for public health, as the number of mosqui-to-borne diseases are increasing continuously across the globe, and spreading in the places, where they have not been reported1. This study was undertaken in and around the Ranchi district of Jharkhand and is the first ever docu-mentation of larvivorous fish fauna in the study area. Lar-vivorous fish has the potential to control various mosquito larval populations. Only few studies have been carried out on larvivorous fishes, investigation of the larvicidal efficacy of some indigenous fish species of the inland eco-system, and utility of local fishes for mosquito control22–27.

In this study, we classified the potential larvivorous fish species on the basis of their larvivorous efficacy and extensive distribution. A high-level of larvivorous poten-tiality was found in C. fasciatus, followed by O. mossam-bica, E. danricus, O. melastigma, P. sophore, P. ticto, R. daniconius, R. elegans, A. panchax and D. rerio. These fish species are being utilized for controlling the mos-quito larval populations in the study area. The findings of present study are in agreement with the findings of previ-ous studies carried out by Sharma et al28, Phukon et al29, Kusumawathie et al30 and Yadav et al31 in different ecosystems. The findings have been confirmed in a lab-oratory based trial carried out in the neighbouring state of Odisha (Rourkela), which showed that A. panchax, O. melastigma, O. mossambicus, Gambusia affinis, D. rerio and E. danricus possess high significant larvi-vorous potential against the mosquito larvae in various conditions31–32.

The study showed the presence of 30 larvivorous fish species in the study area belonging to seven orders, 10


Table 2. Habitat diversity, abundance, and larvivorous potential of the larvivorous fish species collected from Ranchi district, Jharkhand, India

Species Habitat diversity Abundance PotentialHabitat Feeding habit Aquatic ecosystems

Oryzias melastigma R, P BF FW Moderate + + + +Channa punctatus S, R, P, T, RF, WC BCF, C FW Less + + +Amblypharyngodon mola S H, SSF FW Less + + +Chela atpar P, R BF FW Less + + +Chela laubuca P, R BF FW Less + +Cirrhinus mrigala R, T BF FW Less + + +Cirrhinus reba S, R, T BF FW Less + +Cyprinus carpio R BCF FW Moderate + +Danio rerio S, P HCF FW Plenty + + + +Esomus danricus P, T, WC O, SCBF FW, BW Plenty + + + +Labeo bata R HCF FW Rare +Labeo calbasu R, P BF FW Less +Labeo rohita R BF FW Less +Oxygaster bacaila S, R, P O, SF FW, BW Rare + +Puntius phutunio S, R SF FW Less + + +Puntius sarana R, T BCF FW Rare + + +Puntius sophore P BF, BCF, P FW Moderate + + + +Puntius ticto P BCF, H, O FW Moderate + + + +Rasbora daniconius P, S, R H, SSF FW, BW Moderate + + + +Rasbora elegans S SF FW Moderate + + + +Nemacheilus aureus S SF FW Moderate +Nemacheilus savona R BF FW Rare +Aplocheilus panchax P H, O, SF FW, BW Less + + + +Notopterus notopterus P C, P, SCF FW, BW Rare + + +Anabas testudineus R, P, SW, RF C, CF, P FW Less + + + Oreochromis mossambica S, WC SF FW, BW Plenty + + + +Channa gachua SW CF, O FW Less + + +Colisa fasciatus R, T, P, RF CF, O FW Moderate + + + + +Colisa lalia R, T, P, RF CF, O FW Rare + +Mystus bleekeri R SF FW Moderate + +Habitat: S—Streams; R—Rivers; P—Ponds; T—Tanks; RF—Rice fields; WC—Water canals; SW—Swamps; Feeding habit: BCF—Bottom columnar feeder; P—Predatory fish; C—Carnivore; H – Herbivore; SSF—Subsurface feeder; BF—Bottom feeder; HCF—Herbivorous column feeder; SF—Surface feeder; SCBF—Surface column bottom feeder; O—Omnivore; SCF—Surface columnar feeder; CF—Columnar feeder; Aquatic systems: FW—Freshwater; BW—Brackish water; Abundance: L—Less; M—Moderate; P—Plenty; R—Rare; and Larvivorous potential: Consumption of larvae/day—100 or more = + + + + +; 75–99 = + + + +; 50–74 = + + +; 25–49 = + +; 1–24 = +.

Fig. 5: Percent occurrence of the fish species targeted in accordance to their use and trade; WF—Weed fish; CL—Cultivable; F—Food fish; OF—Ornamental fish; MD—Medicinal value.

Fig. 6: Percent occurrence of the fish species according to their major threats to population & habitats; T–Trade; F–Fishing; Lh–Loss of habitat; Oe–Overexploitation; Sn–Siltation; Hi–Human interference; D–Disease;Pu—Pollution.


families, and 21 genera. Order Cypriniformes was found to be the most ascendant group of seven orders, and fam-ily Cyprinidae was found to be the most ascendant family among other nine families. The findings of the present study are similar to the reports of the earlier studies carried out by Krishna et al15 and Raju et al33. Fish fauna is facing a serious threat due to several anthropogenic activities. Of the threats identified, T, Hi, F, overexploitation (Oe), and pollution (Pu) were the major cause of concern. Based on the report of the CAMP workshop on freshwater fishes of India, 18 fish species, viz. C. punctatus, Chela atpar, C. laubuca, C. mrigala, D. rerio, E. danricus, L. bata, L. calbasu, L. rohita, P. sophore, P. ticto, R. daniconius, R. elegans, N. savona, N. notopterus, C. fasciatus, C. lalia, and M. bleekeri were found at LRnt due to the negative effects of above mentioned threats. Maximum of the fish species reported in the study area was exploited for trade, which has caused biodiversity loss and affected the fish fauna status, as fish, is the key species in majority of the aquatic ecosystems34.

This study observed the dominance of omnivorous fish (23.3%) followed by carnivores (10%); which in-dicate an apparent sign of the suitability of the habitat for larvivoracity of the fish. All fish species were found to inhabit in the freshwater region because of the evolu-tionary process by which biological populations evolve in the habitats prefer a salinity <0.05%. Ornamental fish production is a popular and its commercial value viable business in many countries all over the world. This study revealed that 16 species were having ornamental value corroborating to the report of an earlier study carried out by Rao et al35. Because of their ornamental value, some of these larvivorous fishes are also exploited directly from the river, and sold to the nearest aquarium shops providing multiple benefits to the stakeholders. Of these larvivorous fish species, 63.3% fishes are used as food (F) and 60% as cultivable (CL), which shows the suitability of the eco-logical characteristics of larvivorous fish development in these provinces, resulting in a feasibility of an integrated vector control approach which substantiates the sugges-tion made by Walker36.

The present study showed that nine fish species having widespread distribution, are abundantly available in the rivers, ponds, streams, water canals, and tanks in the study area, which indicates their sustainability and capability of tolerance to a wide range of ecologi-cal conditions. Labeo bata, O. bacaila, P. sarana, N. sa-vona, N. notopterus, and C. lalia were rarely observed in the study area during sampling, which might be due to their specific feasibility with the habitat as suggested by Keith37.

CONCLUSION

Of the 30 larvivorous fish species observed in the study area, 10 fishes, namely C. fasciatus, O. mossam-bica, E. danricus, O. melastigma, P. sophore, P. ticto, R. daniconius, R. elegans, A. panchax, and D. rerio showed high-level larvivorous potentiality and were found abun-dantly in the local water bodies, and therefore, could play a significant role in controlling mosquito breeding in the area. These native larvivorous fishes could be used in the integrated mosquito control programmes. However, there is an increasing threat on fish fauna due to several anthro-pogenic activities like fishing, human interference, loss of habitat, overexploitation, pollution, siltation, trade, and diseases are causing the threat to the fish fauna. Conserva-tion measures need to be implemented against these fac-tors for developing a comprehensive action plan.

Therefore, it is suggested to intensify research studies on various aspects of fish biology and its role in mosquito control around the Ranchi district of Jharkhand. Strate-gies like periodic surveys and monitoring, demarcation of breeding sites, creating public awareness should be adopt-ed. Field level research and studies on the efficacy of these fishes as potential larval control agents are scanty. Further, studies aimed to assess the sustainability and efficacy of these fish species at the field level could be helpful in the vector management approaches against the mosquitoes.

ACKNOWLEDGEMENTS

The authors are thankful to the local people in the study area of Ranchi district of Jharkhand for their co-operation and also grateful to the Director, National In-stitute of Malaria Research (NIMR), New Delhi and the Directorate of Fisheries. Funding for the study was provided from the Integrated Diseases Vector Control (IDVC) project of NIMR.

Conflict of interest The authors declare that they have no competing

interests.

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Correspondence to: Dr Manoj Kumar Das, Scientist E, ICMR–National Institute of Malaria Research, Field Unit, Itki, Ranchi–835 301, Jharkhand, India.

E-mail: [email protected]

Received: 4 November 2017 Accepted in revised form: 23 January 2018

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