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WELCOME

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MICRO PROPAGATION IN BANANA

AND POMEGRANATE

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Created by…………….NAME:- digambar holeREG NO:- 2009/063 Department Of Botany & Plant Tissue Culture(ELBOT) College Of Agriculture Pune-05

Introduction. Why do micropropagation?Advantages of micropropagation.General steps of micropropagation.Varieties grown in India.

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content

Banana is a globally important fruit crop with 97.5 million tones of production.

India produces about 26.217 million tones of Banana from an area of 0.709 Mha., with an average productivity of 37.0 mt/ha.

Micropropagation is refers to rapid asexual in vitro multiplication of a desired plant of banana.

In India only 7 percent area under the tissue culture plantlets and remaining 93percent propagated by sucker.

New entrepreneurs having sound scope in production of banana micropropagated plantlets. 5

INTRODUCTION

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Leading Banana Producing Countries

Source: National Horticulture Board (NHB) 2011-12

Indian Production of Banana State wise Production of Banana in India

Source: National Horticulture Board (NHB) 2011-127

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What is MICROPROPAGATION ?

A whole plant can be regenerated from a small tissue or plant cells in a suitable culture medium under controlled environment. The plantlets so produced are called tissue-culture raised plants.

A single explant can be multiplied into several thousand plants in less than a year – this allows fast commercial propagation of new cultivars.

Once established, a plant tissue culture line can give a continuous supply of young plants throughout the year.

In plants prone to virus diseases, virus free explants (new meristem tissue is usually virus free) can be cultivated to provide virus free plants.

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Why do micropropagation ?

Plant ‘tissue banks’ can be frozen, then regenerated through tissue culture.

Plant cultures in approved media are easier to export than are soil-grown plants, as they are pathogen free and take up little space (most current plant export is now done in this manner).

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Cont.…

1.Rapid multiplication

2.Requirement of limited mother stock

3.Product uniformity

4.Season independent production

5.Agronomic advantages

6.Plant exchange

7.High returns

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ADVANTAGES OF micropropagation

12Source : Cadila pharmaceuticals limited

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Stage 0 : Selection and maintenance of stock plants for culture initiation

EXPLANT

SHOOT TIPS FROM YOUNG SUCKER

APICAL MERISTEM (1-2 Cm3)

SURFACED STERILIZATION

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Stage- I : Initiation and establishment of aseptic culture

Source: Jain Irrigation Systems Ltd

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Initiation

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Stage –II : Multiplication of shoot

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Stage -III: Rooting

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Stage –IV: Acclimatization / Hardening

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Secondary hardening

Plants transferred to nursery bags

Kept for 6 to 8 weeks under 50% shade

Regular foliar sprays

Variation if observed is discarded

Plant ready for sale (1 feet height)/7 leaf stage

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BANANA PLANTS READY FOR SALE

An ideal tissue culture raised plant should:

Be 30 cm in height and have a pseudostem circumference of 5.0-6.0 cm after 60 days of total hardening.

Have 4-5 photosynthetically active leaves and inter-foliar space must be not less than 5.0 cm.

Have approximately 25-30 more than 15 cm active roots at the end of secondary hardening.

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Ideal tissue culture raised plant

Be free from any visual symptoms of leaf spot, pseudostem rot and physical deformations;

Be free from root pathogens like Erwinia, nematode

lesions and root knots.

Random checking of roots is essential to ensure health of plantation.

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Cont….

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Hand

Finger

Source: Jain Irrigation Systems Ltd

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Suckers Tissue culture

Source: Jain Irrigation Systems Ltd

Sl.No

Parameters TC propagated

bananas

Sucker propagated

bananas

Comparison

percentage

1 Mean yield (bunches/ha) 2,663 2,416 -2 Mean price received (/bunch) 94.47 76.42 -3 Value of main product (/ha) 2,51,573 184,630 -

4 Value of by-product (/ha) 1729 2,518 -5 Gross income (/ha) 2,53,302 187,149 26%

6 Total expenses (/ha) 1,41,040 108,294 23%

7 Net income (/ha) 1,12,262 78,855 30%

8 Cost of production per bunch 52.31 43.78 -9 Net income per bunch 42.16 32.64 -

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Comparative income from tissue-culture and sucker-propagated bananas

Source: Hanumantharya et al. (2009)

State Varieties grownMaharashtra Grand naine, safed velchi, (AB), Dwarf Cavedish,

Shindhuri, Ardhapuri, Lalvelchi (Mysore), Rajeli (Plantain).

Gujarat Grand naine, Dwarf Cavedish, Lactan, Harichal, Gandevi Selection.

Tamil nadu Grand naine, matti, sevvazhai (red banana), ney poovan, robusta poovan (mysore), sthali (silk), virupakshi (pome), pachanadan, nedran, sakkai.

Andhra Pradesh Grand naine, Dwarf Cavedish, robusta.

Assam Jahaji, borjahaji, manjahaji, robusta, honda, chenichampa, alpan, malbhoj (silk), jatikol (mysore) manohar, bhimkol.

Bihar Dwarf Cavedish, chinai, chenichampa, alpan (mysore), malbhoj (silk), gauria .

Karnataka Grand naine, Dwarf Cavedish, poovan (mysore), robusta , elakki bale.

Kerala Njali poovan, red banana, nendran (plantain), palayankondan (mysore) poovan silk, monthan.

West bengal Amrit sagar, giant governor, lacton, mortman (silk), champa (mysore)

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Varieties grown in India

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MICROPRAPOGATION in POMEGRNATE

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SELECTION OF EXPLANT FOR MICROPRAPOGATION OF

POOMEGRANATE

Shoot tips of about 3-5 cm long were collected from mature trees.

Cleaned under running tap water.

And give treatment of bavistin for 30minuts for kill all fungal infections on explant

Wash it again 2-3 times to remove all resedues of bavistin

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Cont….After washing it again wash with twin20 lab Detergent.

And take material under laminar airflow cabinet.

In laminar airflow cabinet give treatment of 0.1% mercury chloride.

After follow all techniques of sterilization it used in micro propagation.

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Culturemedia for pomegranateMS Media used for micro propagation of pomegranate we add some other chemicals in this viz.

media supplemented with organic acids and vitamins.Sucrose was added at 30.0 g/l and Myo-instol at 0.1g/l.

pH of the prepared media was then adjusted to 5.6- 5.8.

Agar-agar powder used for solidification of media For establishment stage,BAP 2mg/l & NAA is used in media.

In media also use of PVP, INISOTOL, Silver nitrate.PVP-as phenol absorbent.

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Cont…..After sterilization that explant transferred in media .

Transfer explant every day in new media for remove phenol from that explant.

It give rooting after 12-15 days.

After rooting subculture it for further mass production.

After rooting and when its potential of cultivation ends it transfer to cocopit for primary hardening.

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Cont….And after primary hardening it transfer for secondary hardening in soil.

Both hardening carry out in polyhouses.

After hardening it ready to sale.

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By this method Micro propagation is carried out in Banana & pomegranate

THANK YOU34