leitz dialux 20 - microscopen- dialux 20.pdfآ  leitz dialux 20 laboratory and research microscope...

Download LEITZ DIALUX 20 - microscopen- Dialux 20.pdfآ  LEITZ DIALUX 20 Laboratory and Research Microscope Instructions

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  • LEITZ means precision - worldwide.

    " = Registered Trademark Des ign subject to alterations without notice.

    ERNST LEITZ Wri:TZLAR GMBH D-6330 Wetzlar Tel. : (06441) 291 Telex : 483 84'3 leiz d

    Subsidiary : Erns! Leitz (Canada) Ltd ., Midland, On tario Leitz Portugal S.A.R.L., Vi la Nova de Famalicao

    List I 512-150/Eng I. I Pr inted in W-Germany I/77/GX/SD

    LEITZ DIALUX 20 Laboratory and Research Microscope

    Instructions

    Leitz instruments are guaranteed for two years

    against manufacturing defects when purchased

    through an authorized dealer and registered

    with E. Leitz, Inc. Rockleigh, N. J., within 10 days of receipt. A registration card is enclosed

    with each instruntent for this purpose.

    512-150/Engl. I

  • LEITZ DIALUX 20 Laboratory and Research Microscope

    Instructions

    Page

    1 Technical description 3 and 38 2 Technical details 6

    Tubes 6

    Revolving nosepiece 6

    Mechanical Stage 7

    Coarse and fine adjustment 7

    Lamp Housing 102 Z 8

    Condensers 9

    Objectives 12

    Eyepieces 13

    3 Assembling the microscope 14 Centring the lamp 17

    4 Preparing the microscope for operation 19

    Centring the condenser 20

    Oil immersion 22

    Transmitted-light darkground 22

    Phase contrast 23

    Microscopic measurements 27 Polarization 28 Transmitted light fluorescence 28

    Incident-light fluorescence 32 5 Care and maintenance 37 6 Accessories 39

    2 5

  • 1 Technical description

    Fig . 1 DI ALUX 20 with Lamp Housi ng 102 Z, UK universal condenser , Mechanica l Stage No. 78 and FSA phototube.

    1, 2 Knurled knobs for lamp adjustment 3 Lamp Housing 102 Z 4 Lamp condenser adj ustment 5 Rotary knob for the fine adj ustment of the

    object stage 6 Rotary knob for the coarse verti cal adjustment

    of the object stage 7 Stand 8 FSA phototube 9 Eyepiece tube with PERIPLAN " eyepiece 10 Filter slot 11 Revol ving nosepiece fo r 5 objectives 12 NPL FLUOTAR"' objectives 13 Mechani cal Stage No. 78 14 UK universal condenser 15 Vertical adjustment of the condenser 16 Controls for the mechani ca l adj ustment of the

    object stage 17 Adjustable condenser stop 18 Fi eld diaphragm

    1 2

    3

    9

    13

    :;;o..,.,..-------14

    ="'----__ 15

  • 2 Technical details

    Tubes

    • - c; -~ 22639 -5 13 R

    22654 -513 R

    6

    Fig_ 2 Binocular tube S

    Binocular observation tube with ad- justable eyepiece tubes for the mech- anical compensation of the tube length with interpupillary distance adjustment. Tube factor 1x

    Fig_ 3 Binocular phototube FSA

    Binocular observation- and phototube with automatic interpupillary distance compensation. - 100% of the light enters the eye-

    pieces L 50% of the light enters the eye-

    pieces, 50 % the phototube 10% of the light enters the eye-

    pieces, 90 % the phototube Tube factor 1 x

    Fig . 4 Revol ving nosepiece

    The revolving nosepiece takes up to 5 objectives. Tube lens factor 1 x and 1.25x respect- ively.

    22951 ·512 R

    • -

    -__ ,....... _ ..::::._ ..:

    "'-. : l -----,--

    22955-51 2 R

    Fig. 5 Larg e Mechanical Stage No. 78

    The mechanical stage measures 200 x 100mm, and has coaxial controls for object movement within an area of 50 x 76mm.

    Fig . 6 Condenser holder

    This vertically adjustable changer has centring screws condenser and an adjustable stop (arrow).

    Fig. 7

    dovetail for the vertical

    Coarse and fine adjustment of the obj ect stage

    The coarse and fine adjustment, ar- ranged on both sides of the stand, ad- justment range 35mm, actuates the ob- ject stage. One interval on the fine- adjustment corresponds to a vertical difference of 2 ,um.

    7

  • 22656 · 514 A

    22962· 514 R

    8

    Fig . 8 Lamp Housing 102 Z

    The Lamp Housing 102 Z accepts tungsten halogen lamps, ultra-high- pressure mercury lamps, and high-pres- sure xenon lamps of up to 100 W. Lamp and mirror are centred separately. The lamp condenser can be horizontally adjusted .

    Fig . 9 Lamp mount w ith 20 W tungsten halogen lamp for the DIALUX 20 EB.

    Condensers

    Fig. 10 SK stand ard cond enser

    1 In terchangeabl e cond enser top 2 Condenser top holder

    1 2

    3

    4

    5

    6

    3 Lever fo r sw inging out the condenser to p. At the same time the lens 6 is swung into the i ll uminating beam;

    4 Aperture di aphrag m lever 5 Dove tai l changer 6 Supplementary lens

    Condenser with dovetail changer, swing-out condenser to holder (1 0.2) and supplementary lens (10.6) . Can be adapted for special purposes with the use of different condenser tops (10.1) to be screwed into the holder.

    22655·513 R

    Fig. 11 UK unive rsa l condenser

    1 Two cen tring sc rews (o nly one visib le) for the li ght rin g adjustment

    2 In te rchangeabl e condenser top 3 Rota ting turret 4 Aperture di aphrag m 5 Dovetail changer 6 Suppl ementary lens

    Condenser with dovetail changer (11 .5) , swing-out condenser top holder, and supplementary lens (11 .6), can be adapted for special purposes. Rotary turrets (11.3) for phase contrast and interference contrast T can be inserted .

    9

  • . Condenser tops for the SK standard condenser and the UK universal condenser UK Universal condenser for phase contrast

    Condenser Uses Condenser Light ring Rotary Objectives

    top top turret engraved: position

    0.90 81.1 condenser top turned out For objectives of apertures < 0.25 (supplementary lens turned in) 0.90 8 1.1 - H (all objectives) Brightfield

    1 8 1.1 1 Phaco 1 Phase contrast 0.90 8 1.1 condenser top turned in For objectives of aperture > 0.25 2 8 1.1 2 Phaco 2 Phase contrast

    (supplementary lens turned out) 3 8 1.1 3 Phaco 3 Phase contrast 4 8 1.1 4 Phaco 4 Phase contrast

    Oil1 .32 condenser top turned in For use with the Oil100/1.32 OF 8 1.1 5 (all objectives) Darkground (supplementary lens turned objective Aperture < 0.75 out) ; immersion oil on front lens of the condenser 0.70 8 4 - H (all objectives) Brightfield

    0.70 8 4 condenser top turned in Intercept distance 4mm. For (supplementary lens turned out) examination of specimens

    mounted on microscope slides thicker than 1 mm.

    1 8 4 1 Phaco 1 Phase contrast 284 2 Phaco 2 Phase contrast 384 3 Phaco 3 Phase contrast 484 4 Phaco 4 Phase contrast

    0.55 8 15 condenser top turned in Intercept distance 15mm. For (supplementary lens turned out) examination of specimens on

    microscope slides thicker than 6mm

    0.55 8 15 - H (all objectives) Brightfield 1 8 15 1 Phaco 1 Phase contrast 2 815 2 Phaco 2 Phase contrast 4 815 4 Phaco 4 Phase contrast

    0.35 8 30 - H (all objectives) Brightfield 0.35 8 30 condenser top turned in Intercept distance 30mm. For 1 8 30 1 Phaco 1 Phase contrast

    (supplementary lens turned out) examination of specimens on 2 8 30 2 Phaco 2 Phase contrast microscope slides thicker than 10mm

    D 0.80 condenser top turned in Darkground; for objectives of (supplementary lens turned out) apertures < 0.75

    D 1.19 condenser top turned in Darkground ; for objectives of supplementary lens turned out) ; aperture < 1.10 immersion oil on front lens of the condenser top

    10 11

  • Objectives

    1__!gl!l! 2 - JW.,.,......:.;::t;;j

    3:=;~.:.,- 4 5-...... ~~ s-....... ~---~~-. 7-s ;;,.;;:;::;::;:::;:;::

    22952-519 R

    Fig . 12 NPL FLUOTAR 100/1.32 OIL

    All LEITZ objectives computed for tube length 160, as well as objectives of primary magnification greater than 16:1 computed for tube length 170 (note the engraved values) can be used on the DIALUX 20. The following data are engraved on the objective mounts: 1 160 (170) : mechanical tube length.

    The distance in mm between the shoulder of the objective and the rim of the tube.

    2 0.17: coverglass thickness. Only specimens under a coverglass (0.17mm thickness) should be ob- served with these objectives. If the figure 0.17 is replaced by a dash (-) specimens may be observed with or without a coverglass.

    3 Normal piano objectives (flattened field of view of up to at least 18mm intermediate image).

    12

    Piano objectives (flattened field of view of up to 28mm intermediate image).

    Repro- duction 1.6:1 25:1 4:1 6.3:1

    ratio

    Col our grey brown red orange

    10:1 16:1 25 :1 40:1 63 :1 100 :1

    yellow bright dark bright dark white green gree n blue blue

    Ordinary achromatic objectives have no additional letters engraved . Objectives for phase contrast ob- servation have the additional de- signation Phaco (all the NPL-FLUO- TAR objectives are engraved in green throughout) and the ind ication of the associated position of the Phaco ring turret of the UK univer- sal condenser (e.g. Phaco 1 = turret position 1 ).

    4 Reproduction ratio: The dimensional ratio of intermediate image and ob- ject (e.g. 100:1).

    5 Numerical aperture : (e.g. /1.32). 6 The aperture indication is followed

    by that of the immersion medium. 7 Colour code see above table. 8 Immersion objectives have an ad-

    ditional black ring = oil immersion or white ring