host-pathogen interactions in multidrug-resistant tuberculosiscentro de investigaciones...

Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales – CITBM www.citbm.pe / [email protected]

Host-pathogen interactions inMultidrug-Resistant Tuberculosis

Study of the Molecular Epidemiology, Host Immune responses and Genome Based prediction and Early Identification of MDR-TB in High

Tuberculosis Burden Settings

MDR-TBNet Consortium


Oportunity

Problem

Determination

ERANET-LAC


➢ Contributing to the control of a problemthat affects a large part of the worldpopulation, generating a positive impact onthe population.

➢ Establish collaboration, scientific andtechnological exchange.

➢ Research and learning


Our Research ConsortiumCoordinator

Centro de Investigaciones

Tecnológicas, Biomédicas y Medioambientales, Peru

IRCCS Ospedale San

Raffaele, Italy

Universidade Federal de

Minas Gerais, Brazil

Hospital Sant Joan de Déu,

Spain

Research Center Borstel,

Germany

BIOASTER, France


Network strengths

Research Center Borstel (Borstel, Germany)• National Mycobacterium Reference Laboratory• WHO Supranational Reference Laboratory

Hospital Sant Joan de Déu (Barcelona, Spain)• Europeanwide renknown research and

technology facility

IRCCS Ospedale San Raffaele (Milano, Italy)• Research hospital in Molecular Medicine• WHO Collaborating Centre in Tuberculosis

Laboratory Strengthening• WHO Supranational Reference Laboratory for TB

Universidade Federal de Minas Gerais (Belo Horizonte, Brazil)• Top10 university Latin America• Molecular and gentyping reference

Bioaster (Lyon, France)• Technology Research Institute dedicated to

infectious diseases and microbiology in health

CITBM (Callao, Peru)• First peruvian RDI oriented center of excellence• Strong molecular epidemiology background


• High prevalence of MDR-TB in Peru and Brazil

• Conventional diagnostic methods are time consuming

• Limit knowledge of immune response to MDR-TB

• MDR-TB host – pathogen interactions not well defined

Introduction


Goal

Host-pathogenfactors in MDR-TB

Earlydetection

MDR-TB Prevention


Cohort study Molecular epidemiology study

Enrolment patients = 24

TB-MDR

(n = 5)

MODS Culture

Baciloscopy

TB Sensible

(n = 10)

Control

(n = 9)


• Nurse training in sample collection and patient enrolment at UNIDEC –CITBM and Hospital Dos de Mayo.

• TB molecular typing, sequencing and data analysis. Molecular and Experimental Mycobacteriology Group. Forschungszentrum Borstel -Germany, June 2017.

• Mycobacterial small regulatory RNAs. Emerging Bacterial Pathogens Unit., S. Raffalele Scientific Institute. Milán Italy, May –June 2017.

• Fellow research at CITBM.

Technological transference 2017


First steps


Our teamCoordinator

Moises Huaman; Leading Scientist (CITBM, Peru)

Paolo Miotto, (IRCCS

Ospedale San Raffaele, Italy)

Daniella Bartholomeu

(Universidade Federal de Minas Gerais, Brazil)

Laia Alsina (Hospital Sant

Joan de Déu, Spain)

Stefan Niemann (Research Center Borstel, Germany)

BIOASTER, France

Coordinator









BIOASTER, France

Moisés

Huamán

(CITBM, Perú)

Paolo Miotto

(OSR, Italy)

Coordinator









BIOASTER, France

Daniella

Bartolomeu

(UFMG, Brazil)

host-pathogen interactions in multidrug-resistant tuberculosiscentro de investigaciones...

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