cells. staining cells staining is a technique used in microscopy to enhance contrast in the...

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Page 1: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Cells

Page 2: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Staining Cells

• Staining is a technique used in microscopy to enhance contrast in the microscope image

Page 3: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Mounting..

• refers to attaching the samples to a glass microscope slide for observation.

• for samples of loose cells (e.g.blood or pap smear) the sample can be applied directly to a slide.

• for larger tissues thinner sections are made

Page 4: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Stains

• Iodine – more contrast between cell structure. Also goes blue black when starch is present.

• Methyl blue – stains nuclei blue

Page 5: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Structure

You were able to see the following in most cells under the light microscope

• Cell wall• Cell Membrane • Cytoplasm• Nucleus• Vacuole• Plastids

Page 6: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Structure

• The following cell structures are only clearly visible through an electron microscope– Mitochondria– Golgi Apparatus– Smooth Endoplasmic reticulum– Rough Endoplasmic reticulum

Page 7: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Cytoplasm

• Cytosol –is the liquid part

• Cytoplasm is the part that contains all the organelle structures

Page 8: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Plant Vacuoles

Page 9: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Plastids – organelles found in the cells of plants.

• Chloroplasts – found in plants. Containchlorophyll. Responsible for photosynthesis.

• Leucoplasts – non-pigmented plastids found predominantly in the roots and non-photosynthetic parts of plants. They become specialised for bulk storage of starch (e.g.banana), protein and lipids.

• Chromoplasts – for pigment synthesis and storage (e.g. red capsicum). Often found in fruits and flower part of plants.

Page 10: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Cytoplasmic streaming

• See wiki

Page 11: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Protoctists or Protist

• See wiki

• P 83 of text

• Be able to draw amoeba, euglena and paramecium.

Page 12: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Book work

• Biological Drawings - 39-40

• Kingdoms – 70; 79-83

• Types of cells-71

• Determining cell size - 72

• Microscopes - 75-78 (overview)

• Cell structure 85-86 ( a lot of this is difficult but good exercise to attempt.)

Page 13: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Prokaryotes

• Bacteria produce enzymes that break down the organic matter into simpler substances that can then be absorbed by the bacteria.

• Draw and label a generalised bacterial cell. Page 79

• How would you identify a bacterium?• Cell Size very tiny. See page 79 estimate

size. Complete page 72

Page 14: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Cell Specialisation

• Activity

• Group the following into something that makes meaning to you?

• Why did you group them this way?

Page 15: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Cell Specialisation

• Activity

• Group the following into something that makes meaning to you?

• Why did you group them this way?

Page 16: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Cell Specialisation

• Group them into animal and plant cells?

• Can you identify any of these cells or what their function is ? What features did you use to categorise them.

Page 17: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Cell Specialisation

• Identify the following cells– The protista– Identify the following specialised animal cells

• Sex cells• Nerve cells• Muscle cells• Ciliated columnar epithelial cells• Red blood cells• White blood cells• Fat cells• Bone tissue

– Identify the following specialised plant cells

• Cells from a leaf of a plant

• Stomata

• Cells that store starch e.g. banana cells

• Vascular cells (xylem and phloem)

• Cells with chromatoplasts

Page 18: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Sex Cells

Page 19: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Nerve cells

Page 20: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Muscle cells

Page 21: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Ciliated columnar epithelial cells

Page 22: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Blood Cells

Page 23: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Bone Tissue

Page 24: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Fat Cells

Page 25: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Plant Cells

Page 26: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Leaf Cells

Page 27: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Cells that store starch e.g. banana cells

Page 28: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Vascular cells (xylem and phloem)

Page 29: Cells. Staining Cells Staining is a technique used in microscopy to enhance contrast in the microscope image

Cells with chromoplasts