cbs edition 88 - · pdf filecbs edition 88 the cbs ... in contrast to 'traditional'...

CBS Edition 88The CBS classification system

1. Reviews and booksa) Books on TLCb) Books containing one or several chapters on TLCc) Books containing frequent TLC information spread over severalchapters of other information

2. Fundamentals, theory and generala) Generalb) Thermodynamics and theoretical relationshipc) Relationship between structure and chrom. behaviourd) Measurement of physico-chemical and related valuese) Optimization of solvent systemsf) Validation of methods

3. General techniques (unless they are restricted to the applicationwithin one or two classification sections)

a) New apparatus/techniques for sample preparationb) Separation materialc) New apparatus for sample application/dosaged) New apparatus/techniques for chromatogram developmente) New apparatus/techniques for pre- or postchromatographicderivatizationf) New apparatus/techniques for quantitative evaluationg) New apparatus/techniques for other TLC steps (distinguishedfrom section 4)

4. Special techniquesa) Automation of sample preparation/applicationb) Automation of complex chromatogram developing techniquesc) Automation, computer application in quantitative chromatogramevaluationd) Combination of TLC with other chromatographic techniquese) Combination of TLC with other (non-chromatographic)techniques...MS, IR...etc.

5. Hydrocarbons and halogen derivativesa) Aliphatic hydrocarbonsb) Cyclic hydrocarbonsc) Halogen derivativesd) Complex hydrocarbon mixtures

6. Alcohols7. Phenols8. Substances containing heterocyclic oxygen

a) Flavonoidsb) Other compounds with heterocyclic oxygen

9. Oxo compounds, ethers and epoxides10. Carbohydrates

a) Mono- and oligosaccharides, structural studiesb) Polysaccharides, mucopolysaccharides, lipopolysaccharides

11. Organic acids and lipidsa) Organic acids and simple estersb) Prostaglandinsc) Lipids and their constituentsd) Lipoproteins and their constituentse) Glycosphingolipids (gangliosides, sulfatides, neutralglycosphingolipids)

12. Organic peroxides13. Steroids

a) Pregnane and androstane derivativesb) Estrogensc) Sterolsd) Bile acids and alcoholse) Ecdysones and other insect steroid hormones

14. Steroid glycosides, saponins and other terpenoid glycosides15. Terpenes and other volatile plant ingredients

a) Terpenesb) Essential oils

16. Nitro and nitroso compounds

17. Amines, amides and related nitrogen compoundsa) Amines and polyaminesb) Catecholamines and their metabolitesc) Amino derivatives and amides (excluding peptides)

18. Amino acids and peptides, chemical structure of proteinsa) Amino acids and their derivativesb) Peptides and peptidic proteinous hormones

19. Proteins20. Enzymes21. Purines, pyrimidines, nucleic acids and their constituents

a) Purines, pyrimidines, nucleosides, nucleotidesb) Nucleic acids, RNA, DNA

22. Alkaloids23. Other substances containing heterocyclic nitrogen

a) Porphyrins and other pyrrolesb) Bile pigmentsc) Indole derivativesd) Pyridine derivativese) other N-heterocyclic compounds

24. Organic sulfur compounds25. Organic phosphorus compounds (other than phospholipids)26. Organometallic and related compounds

a) Organometallic compoundsb) Boranes, silanes and related non-metallic compoundsc) Coordination compounds

27. Vitamins and various growth regulators (non-peptidic)28. Antibiotics, Mycotoxins

a) Antibioticsb) Aflatoxins and other mycotoxins

29. Pesticides and other agrochemicalsa) Chlorinated insecticidesb) Phosphorus insecticidesc) Carbamatesd) Herbicidese) Fungicidesf) Other types of pesticides and various agrochemicals

30. Synthetic and natural dyesa) Synthetic dyesb) Chloroplasts and other natural pigments

31. Plastics and their intermediates32. Pharmaceutical and biomedical applications

a) Synthetic drugsb) Pharmacokinetic studiesc) Drug monitoringd) Toxicological applicationse) Plant extracts, herbal and traditional medicinesf) Clinico-chemical applications and profiling body fluids

33. Inorganic substancesa) Cationsb) Anions

34. Radioactive and other isotopic compounds35. Other technical products and complex mixtures

a) Surfactantsb) Antioxidants and preservativesc) Various specific technical productsd) Complex mixtures and non-identified compounds

36. Thin-layer electrophoresis37. Environmental analysis

a) General papersb) Air pollutionc) Water pollutiond) Soil pollution

38. Chiral separations

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1. Reviews and books

88 001 S. NYIREDY, Ed.: Planar Chromatography - A Retrospective View for the Third Millennium. Springer ScientificPublisher, Budapest, 2001, 614 pages, ISBN 963 699 157 X. A detailed overview inclusive newest information onplanar chromatography is given. In 29 chapters topics are covered by contributors from many different countriesconcerning the technique and instrumentation of planar chromatography, some specific fields of its application andsome fundamental theoretical considerations. It offers a wide range of techniques; some of them are used only fordistinct analytical tasks. See page 44

1

2. Fundamentals, theory and general

88 002 M.B. ALEKSIC, D.A. AGBABA, D.M. MILOJKOVIC, Z.L. TESIC*, (*Fac. Chem., Univ. Belgrade P.O. Box 158 11001Belgrade, Yugoslavia): Salting-out thin-layer chromatography of some angiotensin converting ensume inhibitorson different sorbents. Chromatographia 53 (Sup.), S-442 - S-444 (2001). Study of the chromatographic behavior ofsix antihypertensive drugs from the class of ACE inhibitors under conditions of salting-out. TLC on silica gel, celluloseand polyacrylonitrile (PANS) as sorbents, and with different concentrations of aqueous ammonium sulfate solution assolvents. It was established that in all cases studied hRf values decrease with increasing salt concentration in thesolvent systems used. A linear relationship of the corresponding RM values and ammonium sulfate content in thesolvent was observed. Proposal of a separation mechanism based on hydrophobic interaction between the sorbent andsorbens.

2, 32

88 003 N.C. DIAS, C.F. POOLE*, (*Dept. of Chem., Wayne State Univ., Detroit, Mi 48202, USA): Compliance of retentiondata on inorganic oxide adsorbents with the solvation parameter model. J. Planar Chromatogr. 14, 160-174(2001). Use of the solvation parameter model for the characterization of the retention properties of a varied group ofsolutes in silica gel thin-layer chromatography (TLC) and in silica gel and aluminium oxide column liquidchromatography. The model was unable to describe retention on silica gel TLC plates with the RM value as thedependent variable for five single-solvent mobile phases. The results were, however, improved fitting the retention datato the simple competition model and separating the solute and solvent contributions to the free energy of adsorption onthe inorganic oxide.

2

88 004 T. KOWALSKA*, G. KOWALIK, W. PRUS, (*Inst. of Chem., Silesian Univ., Szkolna Str. 9, Pl - 40-006 Katowice,Poland): Thermal instability of the selected TLC-type alkyl chemically bonded stationary phases and itsphysical implications. Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp. 75-78.Differential scanning calorimetry and other physico-chemical investigations of chemically bonded phases (RP-8, RP-18,CN and DIOL) at elevated temperatures leading to the conclusion that a weak affect of thermal dissociation of the C-Hbonds which occurs with the chemically bonded aliphatic ligands irradiated with the high-power neodymium laser of aRaman spectrophotometer results in a massive effect of the Raman scattering, enabling rapid and reliable estimation ofthe density of coverage of the silica matrix with the respective aliphatic ligands.

2d

88 005 SZ. NYIREDY, (Res. Inst. for Med. Plants, Lupaszigeti ?t 4, H-2011 Budakal·sz, Hungary): Systematic methoddevelopment in classical and forced-flow planar chromatography. Proc. Intern. Symp. on Planar Separations,Planar Chromatography 2001, pp. 137-148. Presentation of the main steps for a systematic optimization of a planarchromatographic method according to the 'PRISMA' system: The first stage is the selection of the stationary phase, thevapor phase, and suitable solvents which can occasionally immediately result in a suitable separation. For most realseparation problems the second stage, optimization of the mobile phase, is also necessary. The third part of methoddevelopment is the selection of the final conditions, e.g. the mode of development, transfer of the mobile phase to anappropriate forced-flow method, and the selection of suitable operating parameters.

2

88 006 B. OSCIK-MENDYK, (Dept. of Adsorption and Planar Chromatography, M. Curie-Sklodowska Univ., M. CurieSklodowska Sq. 3, 20-031 Lublin, Poland): Solvation effects in liquid adsorption chromatography with mixedmobile phases. J. Planar Chromatogr. b, 178-182 (2001). Evaluation of molecular interactions in liquid adsorptionchromatography with mixed mobile phases. Two equations based on different retention mechanism models wereverified experimentally. TLC on silica gel; development in horizontal chambers with cyclohexane - carbon tetrachloride,cyclohexane - ethylene chloride, n-heptane - ethylene chloride, and n-heptane - acetone.

2e



88 007 A. PETRUCZYNIK, M. WAKSMUNDZKA-HAJNOS, A. HAWRYL, (Dept. of Inorg. and Anal. Chem., Med. Univ.,Staszica 6, 20-081b Lublin, Poland): Retention mechanism of model compounds on polar bonded stationaryphases in reversed phase systems. Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp.299-300. The results of retention of several model substances (phenols, anilines and quinoline bases) obtained on polarbonded stationary phases such as cyanopropyl, diol and aminopropyl in aqueous eluents of different concentrationswere fitted into three equations (Soczewinski and Wachtmeister, Schoenmakers and coworkers andSnyder-Soczewinski) in order to find the best equation to describe retention-mobile phase concentration relationships inreversed phase systems on polar bonded phases.

2c

88 008 M. PROSEK*, A. GOLC-WONDRA, I. VOVK, (*Nat. Inst. of Chem., Hajdrihova 19, Sl-1000 Ljubljana, Slovenia): Uncertainty in TLC. Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp. 91-101. HPTLC ofsodium glutamate on silica gel. Quantitative TLC is considered as a typical separation technique where evaluation ofuncertainty can be done with seriously planned validation. The contributions of sources calculated according toguidelines are so small that their effects on the final results are negligible.

2f

88 009 A. PYKA, (Silesian Acad. of Med., Fac. of Pharm., 4 Jagiellonska Street, PL-41-200 Sosnowiec, Poland): Theapplication of topological indices in TLC. J. Planar Chromatogr. B, 152-159 (2001). Review of the use of topologicalindices based on the adjacency and distance matrixes to predict the Rf and RM values, and the physicochemicalproperties of a variety of groups of organic compounds investigated by TLC. (131 references).

2c, 1

88 010 I. VOVK*, M. PROSEK, J. GIVKES, M. FRANKO, D. BOCANIC, (*Nat. Inst. of Chem., Hajdrihova 19, 1000 Ljubljana,Slovenia): Controlled conditions - a future for TLC. Proc. Intern. Symp. on Planar Separations, PlanarChromatography 2001, pp. 155-161. Photoacoustic spectroscopy was applied to investigate the effect of secondarychromatography on the in-depth distribution of compounds inside the sorbent on two types of TLC plates which weresubject to different drying conditions. All investigated compounds demonstrated nonuniform vertical distribution with thetendency of considerably higher concentrations closer to the surface. TLC and HPTLC of a test dye mixture on silica gelwith toluene.

2c

3. General techniques

88 011 H.E. HAUCK*, O. BUND, W. FISCHER, M. SCHULTZ, (*Merck KGaA, D-64271 Darmstadt, Germany): Ultra-thin layerchromatography (UTLC) - a new dimension in thin-layer chromatography. J. Planar Chromatogr. 14, 234-236(2001). In contrast to 'traditional' layers used in TLC and HPTLC, the new sorbent is no longer based on granularmaterial, but consists of monolithic layers with a defined pore structure based on a silica gel matrix. Furthermore, nobinder is needed to fix the layer on the plate. The layer thickness of approx. 10 µm is considerably less than that ofconventional layers. The exciting properties of the new UTLC silica gel plates, especially their selectivity and separationefficiency, reduction of analysis time and solvent consumption, is demonstrated by separations of steroids, pesticides,and some dyes.

3b

88 012 R.E. KAISER, (Inst. of Chromatography, DE-67085 Bad D¸rkheim, Germany): Digital video processing in PLC: Toolsand development - a break-through. Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp.149-153. Essential values in optical resolution of cameras and digital photo systems, computer memory and processorspeed, data transmission speed and costs of mass memory have been reached in the last years and are now availablewhich are the prerequisite for PLC digital video quantitation.

3f

88 013 G. KOWALIK, T. KOWALSKA*, (*Inst. of Chem., Silesian Univ., 9 Szkolna Street, 40-006 Katowice, Poland): Study ofthe hydrolytic cleavage of alkyl ligands from the surface of chemically bonded stationary phases by means ofselected analytical techniques. J. Planar Chromatogr. 14, 224-233 (2001). Review of findings on the susceptibility ofthe organic chemically bonded ligands to hydrolytic cleavage from the silica matrix for the most common TLC-typestationary phases, i.e. RP-18, RP-8, CN and DIOL. This process was induced by repeated (i.e. one-, two-, four-, andeightfold) development of these phases with mixed mobile phases comprising methanol, water, and buffers of differentpH. Quantification of the extent of hydrolytic cleavage of the different ligands from the silica gel surface was basicallyachieved by means of Raman spectroscopy, although other analytical techniques like elemental (combustion) analysis,thermogravimetry, and differential scanning calorimetry were also employed. Also investigation of the factors influencingthe efficiency of the hydrolytic cleavage.

3b, 2d



88 014 S. KREIBIK*, V. COMAN, C. MARUROIU, G. MIHAILESCU, S. PRUNEANU, (*Nat. Inst. of Res. and Development forIsotopic and Molecular Technologies, P.O. Box 700, Cluj-Napoca 3400, Romania): Enhancement of mobile phasevelocity in TLC by means of an external alternating electric field. J. Planar Chromatogr. 14, 355-359 (2001).Increase of velocity and migration distance of the mobile-phase front in porous media by application of an externalelectric field. TLC on silica gel with benzene - methanol 3:2.

3d

88 015 I. MINCSOVICS, B. DALMADI KISS, G. MOROVJAN, K. BALOGH NEMES, I. KLEBOVICH*, (*EGIS PharmaceuticalsCo. Ltd., Andor u. 60, 1119 Budapest, Hungary): A new tool in metabolism research - Combination of OPLC on-lineradioactivity detection with HPLC-RD technique. Proc. Intern. Symp. on Planar Separations, PlanarChromatography 2001, pp. 113-126. Analytical OPLC of a 14C-labeled compound extracted from body fluids on silicagel with e.g. butanol - acetic acid - water 4:1:1. Detection by digital autoradiography.

3e

88 016 S.P. MUSTOE*, S.D. McCROSSEN, (Glaxo Smith Kline, Old Powder Mills, Tonbridge, TN11 9AN, UK): A comparisonbetween slit densitometry and video densitometry for quantitation in thin-layer chromatography. Chromatographia 53 (Sup.), S-474 - S-477 (2001). Study of linearity, precision and detection limit for some test dyes tocompare the performance of slit with video densitometry. Results show that video densitometry has equivalent precisioncompared to slit densitometry, however, has a broader linear range and better sensitivity for the compounds studied.Other attributes of video densitometry are highlighted.

3f

88 017 S. MUSTOE*, S. McCROSSEN, (*GlaxoSmithKline, Old Powder Mills, Tonbridge, Kent TN11 9AN, UK): TLC imagecapture and analysis by use of a prototype device for visualizing fluorescence. J. Planar Chromatogr. 14,252-255 (2001). Evaluation of a novel, low-cost imaging device for use in TLC - a commercial flat-bed scanner modifiedby inclusion of a 366-nm UV light source. After separation of a test dye images of native fluorescence were recorded;video densitometry was performed on the digital data obtained and compared with slit densitometry measurements. Theimages obtained were high quality, easily processed and archived. The spatial and intensity resolution of the recordedimages were greater than those recorded using current commercial TLC video documentation equipment employinganalog CCD camera technology.

3f

88 018 V.B. PATIL, M.V. GARAD*, (Reg. For. Sci. Lab., State of Maharashtra, Cantonment, Aurangabad-431 002, India): Anew chromogenic spray reagent for the detection and identification of monocrotophos. J. Planar Chromatogr.14, 210-212 (2001). HPTLC of monocrotophos and other organophosphorus insecticides (malathion, ethyl parathion,methylparathion, fenitrothion fenthion, phorate, dimethoate, phosphamidon, dichlorvos), organochlorine insecticides,carbamate insecticides, pyrethroid insecticides on silica gel in a pre-saturated chamber with chloroform - acetone 7:3.After drying the plate is sprayed with a 20% anhydrous sodium carbonate solution then with 0.5% chloranil (in acetone)reagent. The reagent is selective for monocrotophos among other insecticides.

3e, 29b

88 019 W. PRUS, J. FABIA, C. DOBOSZ, B. SZLOSARCZYK, A. STRZADALA, T. KOWALSKA*, (*Inst. of Chem., SilesianUniv., Katowice, Poland): On the thermal instability of selected TLC-type alkyl chemically bonded stationaryphases and on its physical implications. J. Planar Chromatogr. 14, 378-383 (2001). Report of experimental resultsrelating to the thermal instability of selected alkyl chemically bonded stationary phases as a result of their irradiationwith a high-power neodymium laser, the light source of a Raman spectrometer used for the investigation. The resultsreported furnish a convincing confirmation of earlier intuitions, described as "vague speculations about the possiblearomatization of these ligands".

3b, 2c

88 020 I.K. RHEE*, M. van de MEENT, K. INKANINAN, R. VERPOORTE, (*Div. Pharmacognosy, Leiden/Amsterdam Cent.Drug Res., Gorleaus Lab, Leiden Univ., P.O. Box 9502, 2300 RA, Leiden, The Netherlands): Screening foracetylcholinesterase inhibitors from Amaryllidaceae using silica gel thin-layer chromatography in combinationwith bioactive staining. J. Chromatogr. A, 915 (1/2), 217-223 (2001). TLC on silica gel with chloroform - methanol4:1. Detection by spraying with the substrate, dye and enzyme (Eltman's reagent). The advantages of the procedurewere that the known inhibitor, galanthamine could be dereplicated at an early stage of the isolation procedure, andthere in no disturbance from sample. Detection limits were found to be between 10 - 200 ng for several knownacetylcholinesterase inhibitors tested. Comparison with Dragendorff's detection reagent.

3e

88 021 J.K. ROZYLO, G. BEREZKIN, I. MALINOWSKA, A. JAMROZEK-MANKO, (M. Curie-Sklodowska Univ., Fac. of Chem.,



88 021 J.K. ROZYLO, G. BEREZKIN, I. MALINOWSKA, A. JAMROZEK-MANKO, (M. Curie-Sklodowska Univ., Fac. of Chem.,Lublin, Poland): Some technical problems of application of solutes on chromatographic plates in TLC. Proc.Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp. 127-136. Examination of the influence of theapplication method of samples on the migration of their components, dimension of spots on the developedchromatogram, resolution and the number of HETP. TLC of a standard mixture of dyes on silica gel with toluene. A newmethod of application is the use of a filter paper: A given volume of solute is applied on the filter paper - in the form of atriangle - using a calibrated micropipette, then by means of the mobile phase the solute is transferred from the filter tothe sorbent layer.

3c

88 022 B. SPANGENBERG*, B. AHRENS, K.-F. KLEIN, (*Univ. App. Sci. Offenburg, Badstrasse 24, DE-77652 Offenburg,Germany): TLC-Analysis in forensic sciences using a diode-array detector. Chromatographia 53 (Sup.), S-438 -S-441 (2001). Presentation of a new and sturdy HPTLC scanner which allows simultaneous registrations of spectra in arange from 198 nm to 612 nm with a spectral resolution of better than 0.8 nm, and on-plate spectral resolution of betterthan 160 µm. The new diode array scanner offers a fast survey over a TLC separation and makes various chemometricapplications possible. Description of a cross-correlation function for identification of compounds by comparison of UVspectra with appropriate library data, which can also be used for purity testing.

3f

88 023 B. SPANGENBERG*, K.-F. KLEIN, (*Univ. of Appl. Sci. Offenburg, Badstrasse 24, DE-77652 Offenburg, Germany): New evaluation algorithm in diode-array thin-layer chromatography. Proc. Intern. Symp. on Planar Separations,Planar Chromatography 2001, pp. 15-21. HPTLC of benzo(a)pyrene on RP-18 with methanol - acetone 8:3.Spectrophotometry with a diode-array spectrophotometer working in the range of 197 to 612 nm with an averageoptical resolution of better than 2.0 nm.

3f

88 024 P. SU, D. WANG*, M. LAN, (*Dept. of Anal. Chem., Shen Yang Pharm. Univ., Shen Yang, 110015, P. R. China): Half-way development - the technique and the device. J. Planar Chromatogr. 14, 203-207 (2001). Description of anew sandwich chamber in which the mobile phase can be applied to any part of the plate. Because the starting positionof the development can be anywhere this mode was called 'half-way development'. The results obtained with mixturesof dyes are satisfactory.

3d

88 025 A.M. WORONTSOV, A.B. MARKOV, V.G. BEREZKIN*, (*A.V. Topchiev Inst. of Petrochemical Synthesis, RussianAcad. of Sci., 29 Lenin av., Moscow 117912, Russia): Application of a mixed adsorbent for flow TLC. J. PlanarChromatogr. 14, 208-210 (2001). Separation of dyes, polycyclic hydrocarbons (phenanthrene, benzopyrene,anthracene, fluoranthene, pyrene, triphenylene, azulene, naphthacene, chrysene, perylene) and a vegetable chlorophyllextract on plates prepared from Piresorb A, silica gel, luminophore and liquid glass as binder with benzene, hexane,benzene - hexane 1:4 and ethyl acetate - acetonitrile 3:7. For flow TLC it is necessary to use TLC plates with sorbentsdifferent from those used in classical TLC.

3b, d, 30, 5b

4. Special techniques

88 026 Y. SU (Su Yin)*, X. XU (Xu Yuchan), (*Guangzhou Municip. Inst. Drug Cont., Guangzhou 510160, P.R. China): (Studyof the condition for the determination of benzylpenicillin potassium or sodium by mecuri-metric titration -influence of hydrolysis time. ) (Chinese). Chinese J. Modern App. Pharm. (Zhangguo Xiandai Yingyong YaoxueZazhi) 18 (15), 385-387 (2001). TLC on silica gel with ethyl acetate - iso-butanol - acetic acid - water 10:2:5:3.Detection by exposure to iodine vapor. Comparison of the finger prints of the hydrolysis solution obtained at differentconditions.

4e

88 027 E. TYIHAK*, L. BOTZ, S. NAGY, B. KOCSIS, E. MINCSOVICS, (*Plant Protection Inst., Hungarian Acad. of Sci., P.O. B.102, H-1525 Budapest, Hungary): BioArena (TM) as a complex bioautographic system. Proc. Intern. Symp. onPlanar Separations, Planar Chromatography 2001, pp. 3-13. OPLC (HPTLC/TLC) of trans-resveratrol,trans-trans-farnesol, and capsaicin on silica gel with e.g. chloroform - methanol - hexane 20:5:2 or chloroform -acetonitrile 2:3 in linear, one- and two-directional and also two-dimensional off-line separation technique. MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide was used for the visualization of antimicrobial activity.Bioautographic detection using an inoculation process with Bacillus subtilis resp. Botrytis cinerea and Uromycesphaseoli.

4e



5. Hydrocarbons and halogen derivatives

88 028 T.H. DZIDO, (Dept. of Inorg. and Anal. Chem., Med. Acad., Staszica 6, 20-081 Lublin, Poland): Temperatureinfluence on retention parameters of aromatic hydrocarbons with polar groups in binary reversed phasethin-layer chromatography systems. Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp.191-200. RP-HPTLC of 45 aromatic compounds (with e.g. hydroxy- or nitro-groups) using binary eluents containingmethanol, acetonitrile or tetrahydrofuran. Visualization under UV 254 and 366 nm. Separation of the solutes at differenttemperatures can be advantageous due to selectivity changes and shortening of the development time. A horizontaldeveloping chamber with temperature control is presented.

5b

88 029 L.S. LITVINOVA*, V.G. IVANOV, V.N. ZGONNIK, (*Inst. of Macromolecular Compounds, RAS, Bolshoi pr. 31, St.Petersburg, 199004 Russian Federation): Correlation between adsorption and solid-phase interaction of fullereneC 60 with carbohydrates. J. Planar Chromatogr. 14, 343-346 (2001). HPTLC of C 60 and C 70 fullerenes on silica geland silica gel plates impregnated with aqueous solutions of glucose, fructose, sucrose, Dextran and DVB. The mobilephase used was hexane after development with toluene to a distance of 0.5 cm to narrow the starting zone. Detectionunder UV 254 and 366 nm.

5a

88 030 I.V. MARUTSENKO, T.V. POLENOVA, E.D. VIRUS, A.G. BORZENKO, I.A. REVELSKY, (Anal. Chem. Div., Chem.Dept., Moscow State Univ., Leninskie Gory, 119899 Moscow, Russia): HPTLC determination of polyaromatichydrocarbons in smoker's hair. Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp.301-308. HPTLC of benzo(a)pyrene, pyrene, fluoranthene, anthracene, fluorene and hair extracts on silica gel withhexane - dichloromethane - nitrobenzene (5:5:x, where x changed from 0.01 ml up to 0.5 ml); best results with anitrobenzene content of 0.1. Quantitation by densitometry at 365 nm. The concentration of PAHs in human hair was inthe range of 0.005-0.2 µg/g hair.

5b

88 031 E. ROETS*, I. QUINTENS, R. KIBAYA, J. HOOGMARTENS, (*Lab. for Pharm. Chem. and Drug Anal., Van Evenstraat4, B-3000 Leuven, Belgium): Quantitative determination of olaquindox in animal feed. J. Planar Chromatogr. 14,347-349 (2001). TLC of olaquindox, on silica gel plates with ethyl acetate - acetone - ethanol 62:40:3; development in asaturated chamber. Quantitative determination by densitometry at 360 nm. The method is also selective for theantibiotics tetracycline and tylosin. New simple, relatively rapid procedure.

5a, 28a

8. Substances containing heterocyclic oxygen

88 032 A.A. AHMED, A.A. MAHMOUD*, A.A. El-GAMAL, (*Dept. of Chem., Fac. of Sci., El-Minia Univ., El-Minia, Egypt): Axanthanolide diol and a dimeric xanthanolide from Xanthium species. Planta med. 65, 470-72 (1999). PreparativeTLC of a new dimeric xanthanolide pungiolide C and the dimeric xanthanolide pungiolide A on silica gel with petroleumether - ether 1:3. Detection under UV.

8b

88 033 C. BODINET, J. FREUDENSTEIN, (Schaper & BrÜmmer GmbH & Co. KG, Bahnhofstr. 35, D-38259 Salzgitter,Germany): Effects of an orally applied aqueous-ethanolic extract of a mixture of Thujae occidentalis herba,Baptisiae binetoriae radix, Echinaceae purpureae radix and Echinaceae pallidae radix on antibody responseagainst sheep red blood cells in mice. Planta med. 65, 695-699 (1999). TLC of maackiain, umbelliferone, cichoricacid and echinacoside on silica gel with ethyl acetate - acetic acid - formic acid - water 100:11:11:27 and formic acid -ethyl acetate - toluene 3:10:10. Detection after spraying with 1% 2-aminoethyldiphenyl borate in methanol and 5%polyethylene glycol 4000 in methanol under UV.

8b

88 034 T. CHEN, J. LI, J. CAO, Q. XU*, K. KOMATSU, T. NAMBA, (*Dept. of Pharm. for Chinese Materia Medica, ChinaPharm. University, 24 Tong Jia Xiang, Nanjing 210009, P.R. China): A new flavone isolated from Rhizoma Smilacisgabrae and the structural requirements of its derivatives for preventing immunological hepatocyte damage. Planta med. 65, 56-59 (1999). Anal. and preparative TLC of dihydroquercetin, resveratrol, and engeletin on silica gelwith chloroform - methanol 5:1. Detection under UV or by spraying with cerium sulfate - 10% sulfuric acid 1:99. AlsoTLC of rhamnose with n-butanol - water - acetic acid 4:2:1.

8

88 035 K. CIMANGA, A. J. VLIETINCK* et al, (*Dept. of Pharm. Sci. , Univ. of Antwerp, Universiteitsplein 1, B-2610 Wilrijk,



88 035 K. CIMANGA, A. J. VLIETINCK* et al, (*Dept. of Pharm. Sci. , Univ. of Antwerp, Universiteitsplein 1, B-2610 Wilrijk,Antwerp, Belgium): Complement-inhibiting constituents of Bridelia ferruginea stem bark. Planta med. 65,213-217 (1999). TLC of (e.g. gallocatechin-(4'-O-7)-epigallocatechin, 3,5-dicaffeoylquinic acid, myricetin, quercetin3-O-glucoside) on silica gel with ethyl acetate - acetic acid - formic acid - water 75: 2:3:20 and n-butanol - acetic acid -water 4:1:5; upper layer. Detection under UV 254 nm, by spraying with 1% vanillin and 5% sulfuric acid in methanoland by spraying with Neu's reagent.

8a

88 036 A.B. FALL, R. VANHAELEN-FASTRE, M. VANHAELEN *, I. LO, M. TOPPET, A. FERSTER, P. FONDU, (*Lab. dePharmacogn. et Bromatologie, Inst. de Pharm. de l'Université Libre de Bruxelles, U.L.B., C.P. 20514, Bd du Triomphe,B-1050 Bruxelles, Belgium): In vitro antisickling activity of a rearranged limonoid isolated from Khagasevegalensis. Planta med. 65, 209-212 (1999). Preparative TLC ofmethyl-1a,2b,3a,6,8a,14b-hexahydroxy-[4,2,1(10,30),1(1,4)] -tricyclomeliac-7-oate on silica gel with hexane -dichloromethane - methanol 15:3:2.

8b

88 037 S. FUSHIJA*, Y. KISHI, K. HATTORI, J. BATKHUN, F. TAKANO, A.N.B. SINGAB, T. OKUYAMA, (*Fac. of Pharm. Sci. ,Tohaku Univ., Aramaki-Aza-Aobayama Aoba-Ku, Miyasi Sendai 980-8578, Japan): Flavonoids from Cleomedroserifolia suppress NO production in activated microphages in vitro. Planta med. 65, 404-407 (1999). TLC of5,4'-dihydroxy-6,7,8,3'-tetramethoxyflavone (8-methoxycirsilineol) and the new5,4'-dihydroxy-6,7,8,3',5'-pentamethoxyflavone on silica gel with hexane - ethyl acetate 1:1 for the analytical separationand acetone - benzene for the preparative separation. Detection under UV.

8a

88 038 J. HOHMANN*, I. ZUPKO, D. REDEI, M. CSANYI, G. FALKAY, I. MATHE, G. JANICSAK, (*Dept. of Pharmacogn.,Albert Szent-Györgyi Med. Univ., P. O. Box 121, H-6701 Szeged, Hungary): Protective effects of the aerial parts ofSalvia officinalis, Melissa officinalis and Lavandula angustifolia and their constituents againstenzyme-dependent and enzyme-independent lipid peroxidation. Planta med. 65, 576-578 (1999). TLC of e.g.luteolin, luteolin-7-O-glucoside, methyl carnosoate on silica gel with chloroform - methanol 49:1 and n-hexane - ether -acetic acid 79:20:49 (prep. TLC), of luteolin with toluene - ethyl acetate - formic acid 10:4:1, of luteolin-7-O-glucosidewith ethyl acetate - formic acid - acetic acid - water 25:2:2:4 and n-hexane - ether - acetic acid 79:20:4. Detection underUV. - Densitometry at 245 nm resp. 327 nm.

8a

88 039 J. HU, Z. JIA*, (Inst. of Org. Chem., Lab. of Appl. Org. Chem. at Lanzhou Univ. of Karachi, Lanzhou, P.R. China): Flammolide: A C-14 carboxylate endesmanolide from Senecio flammens. Planta med. 65, 386-388 (1999).Analytical TLC of the new endesmanolide flammolide on silica gel with petroleum ether - ethyl acetate 10:1 andpreparative TLC with chloroform - methanol 30:1; 4 x developed. Detection under UV 254 nm.

8b

88 040 S.-U. KIM, E.-I. HWANG, J.-Y. NAM, K.-H. SON, S.-H. BOK, H.-E. KIM, B.-M. KWON*, (*Protein Regulator R. U., KoreaRes. Inst. of Biosci. and Biotechn., KIST, P. O. Box 115, Yusung, Taejou, Korea): Inhibition of chitin synthase II bycatechins from stem bark of Taxus cuspidata. Planta med. 65, 97-98 (1999). Preparative TLC of (+-)-catechin and(-)-epicatechin on silica gel with 25% dichloromethane/methanol. Detection under UV.

8

88 041 M. KRAUZE-BARANOVSKA*, P. SOWINSKI, (*Dept. of Pharmacogn., Med. Univ. of Gdansk, ul. Gen. J. Hallera 107,PL-80-416 Gdansk, Poland): 2,3-Dihydrobiflavone from Gingko biloba. Planta med. 65, 482 484 (1999).Preparative TLC of sciadopitysin, gingketin, isogingketin, bilobetin on silica gel in a sandwich chamber modified byRuminski with benzene - pyridine - formic acid 36:9:5. Detection under UV.

8a

88 042 B.-M. KWON*, H.-J. JUNG, J.-H. LIM, Y.-S. KIM, M.-K. KIM, Y.-K. KIM, S.-H. BOK, K.-H. BAE, I.-R. LEE, (*ProteinRegulator RU, Korea Res. Inst. of Biosci. and Biotechn., KIST, P.O. Box 115, Yoosang, Taejou, Korea): Acyl-CoA:Cholesterol acyltransferase inhibitory activity of lignans isolated from Schisandra, Machilus and Magnoliaspecies. Planta med. 65, 74-76 (1999). Peparative TLC of 15 lignans (i. a. gomisin N, A, B, K3, J, L2,benzoylisogomisin O, angeloylgomisin H, sigloylgomisin P, schisandrin, schisantherin, licarin A, D, machiline G,GERI-BP001/M) on silica gel with 20 - 40% ethyl acetate - hexane.

8b

88 043 B. MIN, S.R. OH, H.-K. LEE, K. TAKATSU, I.-M. CHANG, K. R. MIN, Y. KIM*, (*Coll. of Pharm., Chungbuk Nat. Univ.,



88 043 B. MIN, S.R. OH, H.-K. LEE, K. TAKATSU, I.-M. CHANG, K. R. MIN, Y. KIM*, (*Coll. of Pharm., Chungbuk Nat. Univ.,Cheongju 361-763, R. of Korea): Sophoricoside analogs as the IL-5 inhibitors from Sophora japonica. Plantamed. 65, 408-412 (1999). TLC of sophoricoside on silica gel with chloroform - methanol - water 5:1:1, on RP-18 withmethanol - water 1:1, of genistein on silica gel with n-hexane - acetone 2:1, of orobol on silica gel with chloroform -methanol 10:1, and of genistin on silica gel with chloroform - methanol - water 50:10: 1 and on RP-18 with methanol -water 1:1. Detection under UV.

8a

88 044 S.A. ROSS, A. ZAGLOUL, A.C. NIMROD, Z. MEHMEDIC, H.N. ELSOHLY*, (*Nat. Center for the Development of Nat.Prod., School of Pharm., Univ. of Mississippi, Univ., MS 38677, USA): A cytotoxic chalcone from Farameasalicifolia. Planta med. 65, 194 (1999). Preparative TLC of 3',4-di-O-methyl-cedrusin on silica gel with ethyl acetateand of calylthropsin with chloroform - ethyl acetate 1:1. Detection under UV 254 nm.

8b

88 045 B. SIMONOVSKA*, I. VOVK, S. ANDRENSEK, T. YRJÖNEN, P. VUORELA, H. VUORELA, (*Nat. Inst. of Chem., Lab.for Food Chem., Hajdrihova 19, Sl-1000 Ljubljana, Slovenia): Isolation of (+)-catechin from oak bark byfractionation of its extract on Extrachrom (R). Proc. Intern. Symp. on Planar Separations, Planar Chromatography2001, pp. 335-341. Isolation of (+)-catechin by rotating chromatography on silica gel with toluene - ethyl acetate - formicacid 35:15:1. TLC on silica gel with ethyl acetate - water - formic acid 17:3:2 resp. n-hexane - ethyl acetate - formic acid20:19:1. Detection under UV 366 nm (flavonoids, phenolic acids), spraying with vanillin/phosphoric acid reagent(catechins, proanthocyanidins) and spraying with 1,1-diphenyl-1-picrylhydrazyl (antioxidants). Videodocumentation at366 nm.

8b, 32e

88 046 L. UDINO, J. ABAUL*, P. BOUREOIS, L. GARRICHOU, H. DURAU, C. ZEDDE, (*Lab. de Chimie des SubstancesNaturelles, U.A.G. Campus de Fouillole, F-97100 Pointe-à-Pitre Cedex, Gouadeloupe, FWI): Lignans from the seedsof Hernandia sonora. Planta med. 65, 279-281 (1999). TLC of lignans (podophyllotoxin, picropodophyllin,deoxypodophyllotoxin, hernandin, podophyllotoxin acetate and 5-methoxypodophyllotoxin, 5-methoxypodophyllotoxinacetate) on silica gel with petroleum ether - ethyl acetate 1:1. Visualization under UV 254 nm.

8b

88 047 S. ZHENG*, S. KANG, Y, SHEN, Z. SUN, (*Dept. of Chem., Northwest Normal Univ., Lanzhou 730070 , P.R. China): Three new C-methylated flavones from Elsholtzia stauntonii. Planta med. 65, 173-175 (1999). Preparative TLC ofi.a. muxiangrine III, 5,7,3',6'-tetrahydroxy-8,2'- dimethoxyflavone on silica gel with chloroform - ethyl acetate - methanol14:4:1. Detection under UV 254 nm.

8a

10. Carbohydrates

88 048 K. ALMSTRUP*, P. POULSEN, I. HILDEN, (*Danisco Cultor Innovation Copenhagen, Langebrogade 1, P.O. Box 17,1001 Copenhagen K, Denmark, [email protected]): A fast method for measurement of branchingenzyme activity using thin-layer chromatography-based phosphorylase a stimulation assay. Anal. Biochem.286, 297-300 (2000). 1-ml aliquots of reaction mixtures were quenched by loading directly on silica gel TLC plates.Separation of residual glucose-1-phosphate from polymeric glucans with n-butanol - isopropanol - acetic acid - water3:12:4:4. Visualization by spraying or dipping with acetic acid - H2SO4 - anisaldehyde 100:2:1.

10, 20

88 049 K. ANDERSON, SU-CHEN LI, YU-TEH LI*, (*Dept. of Biochem., Tulane Univ. School of Med., 1430 Tulane Avenue,New Orleans, Louisiana 70112, [email protected]): Diphenylamine-aniline-phosphoric acid reagent, aversatile spray reagent for revealing glycoconjugates on thin-layer chromatography plates. Anal. Biochem. 287,337-339 (2000). Visualization of glycoconjugates by spraying with diphenylamine-aniline-phosphoric acid reagent.Heating for 25 min at 120?C. Sensitive reagent for neutral sugars, sialic acids, 3-deoxy-D-manno-octulosonic acid,2-keto-3-deoxy-D-glycero-D-galactonononic acid, and hexosamines found in glycoconjugates (cello- andchito-oligosaccharides, neutral glycosphingolipids, gangliosides).

10, 31

88 050 L.W. DONER, (U.S. Dept. Agr., Agr. Res. Serv., Eastern Regin. Res. Cent., 600E, Mermeid Lane, Wyndmoor, PA19038 USA): Determining sugar composition of food gum polysaccharides by HPTLC. Chromatographia 53(9-10), 579-581 (2001). TLC on silica gel (Si 50000) with propanol - water - NH3 10:5:1 with traces of triethylamine,after acid hydrolysis. Detection by spraying with 5% sulfuric acid and 6.5 mM NI (Inaphthyl) ethylenediaminedihydrochloride in methanol and heating at 100°C.

10b



88 051 J. FLIEGER*, H. SZUMILO, (*Dept. of Inorg. and Anal. Chem., Med. Acad., 20-081 Lublin, Staszica 6, Poland): Theeffect of metal salts on the separation of carbohydrates by TLC. J. Planar Chromatogr. 14, 338-342 (2001). TLCof carbohydrates on silica gel plates impregnated by dipping in methanolic solutions or by use of mobile phaseadditives; the mobile phases were mixtures of acetonitrile, 2-propanol, or acetone with water or aqueous solutions ofthe metal salt. Plates were developed in horizontal chambers. Post-chromatographic derivatization was performed byspraying with a solution of diphenylamine (2 g), aniline (2 mL), 85% phosphoric acid (10 mL), and methanol (100 mL)and drying for 5 min at 105°C.

10a

88 052 U. HUBL*, G.C. SLIM, O.V. ZUBKOVA (*Ind. Res. Ltd., Gracefield Res. Centre, P.O.-Box 31-310, Lower Hutt, NewZealand, [email protected]): Thin-layer chromatography and polyacrylamide gel electrophoresis-based assays forsialyltransferases using tetramethylrhodamine-labeled acceptors. Anal. Biochem. 285, 92-99 (2000). TLC ofdesalted incubation mixtures on RP-18 with acetonitrile - water 2:3. Determination with a gel documentation system.Comparison of TLC with polyacrylamide gel electrophoresis-based method.

10, 20

11. Organic acids and lipids

88 053 R.M. ALI, P.J. HOUGHTON*, (*Pharmacogn. Res. Lab., Dept. of Pharm., King's Coll. London, Manresa Road, LondonSW3 6LX, U.K.): A new phenolic fatty acid ester with lipoxygenase inhibitory activity from Jacaranda felicifolia. Planta med. 65, 455-457 (1999). Preparative TLC on silica gel plates of 2-(4-hydroxyphenyl)ethyl1-dodecyl-octadecanoate on silica gel with hexane - ethyl acetate 7:3 and of ursolic acid with chloroform - ethyl acetate5:2. Detection under UV 254 nm.

11a

88 054 L. BERNA, B. ASFAW, E. CONZELMANN, B. CERNY, J. LEDVINOV¡, (Inst. for Inherited Metabol. Deseases, First Fac.of Med. and Univ. Hospital, Charles Univ., CZ-12808 Prague 2, Czech Republic): Determination of urinary sulfatidesand other lipids by combination of reversed-phase and thin-layer chromatographies. Anal. Biochem. 269,304-311 (1999). HPTLC of total urinary lipids on silica gel with chloroform - methanol - water 65:25:4. Thechromatogram was first sprayed with primuline, and sphingomyelin was determined fluorometrically at 365 nm/>400nm. Subsequently, sulfatides were stained with azure A or orcinol. Densitometry in reflection mode at 610 or 546 nm,respectively.

11, 32

88 055 K.K. HEDBERG, B. COGAN, G.B. BIRRELL, O.H. GRIFFITH*, (*Inst. Mol. Biol. & Dep. Chem., Univ. Oregon, Eugene,OR 97403, USA): Sensitive fluorescent quantitation of myo-inositol 1,2-cyclic phosphate and myo-inositol1-phosphate by high-performance thin-layer chromatography. J. Chromatogr. B, 757 (2), 317-324 (2001).Description of a non-radioactive micro-assay for the cyclic phosphodiesterase reaction catalyzed by Bacillus cereusphosphatidylinositol-specific phospholipase C, involving HPTLC on silica gel to resolve the substrate (myo-inositol1,2-cyclic phosphate and the product (myo-inositol 1-phosphate), followed by detection with a leadtetraacetate-fluorescein stain. Quantitation by analysis of the fluorescent plate image with a commercial phosphoimagerand associated software, for sample in the rage of 0.1 to 50 nmol.

11, 3a

88 056 K. KISHIMOTO, R. URADE, T. OGAWA, T. MORIYAMA* (*Res. Inst. for Food Sci., Kyoto Univ., Gokasho, Uji, Kyoto611-0011, Japan, [email protected]): Nondestructive quantification of neutral lipids by thin-layerchromatography and laser-fluorescent scanning - Suitable methods for "Lipidome" analysis. Biochem. Biophys.Res. Comm. 281, 657-662 (2001). TLC of monoglycerides, diglycerides, free cholesterol, fatty acids, triglycerides andcholesterol esters on silica gel 60 using hexane - diethyl ether - acetic acid 70:30:1. After drying for 30 min seconddevelopment in the same solvent mixture. Spraying with 0.00003% rhodamine 6G in 95% ethanol. Scanning bylaser-excited fluorescent detection with green laser (532 nm) for excitation, and an emission filter of 555 nm. Detectionlimit 20 mg of lipid.

11, 32

88 057 I. MAREVKOV*, R. TARANDJIISKA, S. PANAYOTOVA, N. NIKOLOVA, (*Inst. of Org. Chem. and Center ofPhytochem., Acad. G. Bonchev Str. Blok 9, 1113 Sofia, Bulgaria): Valuation of argentation TLC densitometry for thedetermination of trans fatty acids in butterfat. J. Planar Chromatogr. 14, 384-390 (2001). TLC of fatty acids (e.g4:0, 6:0, 8:0, 10:0, 12:0, 14:0, 16:0, 17:0, 18:0, 20:0, 22:0, 24:0, 26:0), butter, oleic type margarines, linoleic typemargarines on silica gel plates impregnated by dipping into a 0.5% (or 1%, where needed) methanolic solution of silvernitrate. Mobile phases used were petroleum - acetone 25:1; 50:1; 50:3; 40:1 or chloroform. Detection after drying byconsecutive treatment with bromine and sulfuryl chloride vapor (30 min each) to ensure correct quantitative charring (at



180-200°C). Quantitation by densitometry at 450 nm in reflectance mode. 11a

88 058 K.YOKOYAMA*, F. SHIMIZU, M. SETAKA, (*Fac. of Pharm. Sci., Teikyo Univ., Sagamiko, Tsukui, Kanagawa 199-0195,Japan): Simultaneous separation of lysophospholipids from the total lipid fraction of crude biological samplesusing two-dimensional thin-layer chromatography. J. Lipid Res. 41, 142-147 (2000). 2-D TLC of lysophospholipids(lysophosphatidic acid, lysophosphatidylcholine, lysophosphatidylethanolamine, lysophosphatidylglycerol,lysophosphatidylinositol, lysophosphatidylserine, sphingosylphosphorylcholine, sphingosine-1-phosphate) on silica gelin the first direction with 1) chloroform - methanol - formic acid - water 60:30:7:3 and in the second direction with 2)chloroform - methanol - 28% NH3 - water 25:29:4:1. To separate lysophospholipids from neutral lipids on the sameplate, a third run was performed with ether in the opposite direction to the second run.

11c

14. Steroid glycosides, saponins and other terpenoid glycosides

88 059 S. FAIZI*, M. ALI, (*H.E.J. Res. Inst. of Chem., Univ. of Karachi, Karachi - 75270, Pakistan): Shaminin: A newflavonol C-glycoside from leaves of Bombax ceiba. Planta med. 65, 383-385 (1999). Analytical TLC of shaminin{2-(2,4,5-trihydroxyphenyl)-3,5,7-trihydroxy-6-C-gluco- pyranosyloxy-4H-1-benzopyrano-4-one} on silica gel with ethylacetate - methanol - water 8:2:1. Detection under UV 254 nm.

14

88 060 C. FELSER, O. SCHIMMER, (Inst. für Botanik und Pharm. Biol., Friedrich-Alexander-Univ. Erlangen-Nürnberg,Staudtstr. 5, DE-91058 Erlangen, Germany): Flavonoid glucosides from Alchemilla speciosa. Planta med. 65,668-670 (1999). Analytical TLC of e.g. quercetin 3-O-b-D-sambubioside-7-O-b-D-glucopyranoside, rutin, quercetin3-O-b-D-sambubioside, luteolin 7-O-b-D-(6-O-a-L-rhamnopyranosyl)-glucopyranoside, isoquercetrin, hyperin, quercitrin,astragalin, luteolin 7-O-b-D-glucopyranoside, kaempferol, 3-O-?-D-glucopyranoside uronic acid, miquelianine, tilirosidon silica gel with ethyl acetate - formic acid - acetic acid - water 100:11:11:27 and 140:11:11:27, toluene - ethyl formiate- formic acid - dichloromethane - ethyl acetate 5:4:1:6:4. Detection under UV, by spraying with diphenylboric acid2-aminoethylester (0.5% in ethyl acetate) and polyethylene glycol 4000 (5% in dichloromethane).

14

88 061 M. GLENSK*, M. CZEKALSKA, W. CISOWSKI, (Dept. of Pharmacogn., Wroclaw Med. Univ., pl. Nankiera 1, 50-140Wroclaw, Poland): Resolution of saponins from ginseng preparation by 2D-TLC with a sorbent gradient. Proc.Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp. 217-222. 2D-TLC separation of saponins andginsenoside Rg1 as standard on RP-18 with 70% methanol in the first direction and after transfer to a silica gel platewith chloroform - methanol - water 35:15:2 in the second direction. Visualization by spraying with anisaldehyde-sulfuricacid reagent, heating at 120°C for 5 min and evaluation in visible light or under UV 365 nm.

14

88 062 C.M. PASSREITER*, M. De CARLO, A. STEIGL, (*Inst. für Pharm. Biol., Heinrich-Heine -Univ., Universitätsstrasse 1,DE-40225 Düsseldorf, Germany): Sesquiterpene lactone glycosides from Arnica longifolia. Planta med. 65,153-156 (1999). TLC of glycoside mixture (11a,13- and 11b,13-dihydro-4H-tomentosin glucoside) on silica gel with ethylacetate - acetic acid - formic acid - water 100:11:11:27. Detection by spraying with anisaldehyde - sulfuric acid. TLC ofaglycones 11a,13 and 11b-13-dihydro-4H-tomentosin on silica gel with toluene - ethyl acetate 1:1. Also acid hydrolysison TLC plates with concentrated hydrochloric acid at 100°C; chromatography with ethyl acetate - methanol - acetic acid- water 2:3:3:2. Detection of the obtained sugars by spraying with thymol-sulfuric acid reagent.

14

88 063 X. SUN, A.T. SUEDEN*, (*Dept. of Chem., Virginia Commonwealth Univ., P.O. Box 842 019, Richmond VA23284-2019, USA): Neoflavonoids from Polygonum perfoliatum. Planta med. 65, 671-673 (1999). Preparative TLCof e.g., 3,4-dihydro-5-hydroxy-7-methoxy-4- (4'-methoxyphenyl)coumarin on silica gel with dichloromethane - hexane -ethyl acetate 6:3:1, of 3,4-dihydro-4-(4'-hydroxyphenyl)-5,7-dihydroxycoumarin, 3,4-dihydro-5-hydroxy-7-methoxy-4-(4'-hydroxyphenyl)coumarin, and 3,4-dihydro-5,7-dihydroxy-4-(4'-methoxyphenyl)coumarin on silica gel with 1.dichloromethane - hexane - methanol 6:3:1 and 2. dichloromethane - methanol 9:1. Detection under UV.

14, 8a

15. Terpenes and other volatile plant ingredients

88 064 A. AGIL*, M. MIRO, J. JIMMENEZ, J. ANEIRAS, M.D. CARACUEL, A. GARCIA-GRANADOS, M.C. NAVARRO, (Dept.



88 064 A. AGIL*, M. MIRO, J. JIMMENEZ, J. ANEIRAS, M.D. CARACUEL, A. GARCIA-GRANADOS, M.C. NAVARRO, (Dept.of Pharm., Sch. of Med., Univ. of Granada, E-18071 Granada, Spain): Isolation of an anti-hepatotoxic principlefrom the juice of Ecballium elaterium. Planta med. 65, 673-675 (1999). TLC of cucurbitacin B(25-(acetyloxy)-2b,16a,20-trihydroxy-9b-methyl-19-nor-10a- lanosta-5,23E-diene-3,11,22-trione) on silica gel withtoluene - ethyl acetate - acetic acid 100:3:0.7. Detection by spraying with vanillin - phosphoric acid reagent.

15a

88 065 C.C. CHEN*, H.-Y. CHEN, M.-S. SHIAO, Y.-L. LIN, Y.-H. KUO, J.-C. ON, (*Nat. Res. Inst. of Chin. Med., No. 155-1.Sec 2, Li Nung St. Peitou, Taipei 112 Republic of China): Inhibition of low density lipoprotein oxidation bytetrahydrofurafuran lignans from Forsythia suspensa and Magnolia coco. Planta med. 65, 709 (1999). TLC onsilica gel of betulinic acid and oleanolic acid with chloroform - methanol 20:1, and of pinoresinol and phillygenin withchloroform - acetone 20:1.

15, 11a

88 066 C. DEMETSOS*, B. STAHL, T. ANSTSSAKI, M. GAZOULI, L.S. TSOUVELEKIS, M. Rallis, (Dept. of Pharm., Lab. ofPharmacogn., Univ. of Athens, Panepistimiopolis, Zografou 15771, Greece): Chemical analysis and antimicrobialactivity of the resin Ladano, of its essential oils and of the isolated compound. Planta med. 65, 76-78 (1999).Analytical TLC of labdane diterpenes (drimane-7,9(11)-diene, ent-3b-acetoxy-13-epi-manoyloxide) on silica gel withn-hexane - ether 49:1.

15a

88 067 K. LIKHITWITAYAWUID*, S. DEJ-ADISAI, V. JONGBUNPRASERT, J. KRUNGKRAI, (*Dept. of Pharmacogn., Fac. ofPharm. Sci. , Chulalongkorn Univ., Bangkok 10330, Thailand): Antimalarials from Stephania venosa and Murrayasimensis. Planta med. 65, 754-756 (1999). Preparative TLC of rubiadin-1-methyl ether(1-methoxy-3-hydroxy-2methylanthrquinone) on silica gel with toluene - ethyl acetate (9:1).

15

88 068 M. MAO, Y. KASHMAN, I. NEEMAN*, (*Dept. of Food Eng, and Biotechn., Technion, Haifa 32000, Israel): Isolationand identification of a new antifungal sesquiterpene lactone from Inula viscosa. Planta med. 65, 281-282 (1999).TLC of tagunin on silica gel with petroleum ether - ether - acetic acid - ethanol 95% 90:20:3:2. Visualization by exposureto iodine vapor.

15a

88 069 Q.-W. SHI, T. ORITANI*, T. SUGIYAMA (*Lab. of Appl. Bioorg. Chem., Div. of Life Sci., Graduate Schol of Agr. Sci.,Tohaku Univ., 1-1 tsutsumidori-Amamiya, Aoba-ku, Sendai 981-8555, Japan): Three novel bicyclic taxanediterpenoids with verticilline skeleton from the needles of Chinese Yew, Taxus chinensis var. mairei ewtazettine-type alkaloids from Galanthus gracilis and Galanthus plicatus subsp. byzantinus. Planta med. 65, 356-359(1999). Preparative TLC of (3E,7E)-2a,10b,13a,20-tetraacetoxy-5a-hydroxy-3,8-secotaxa-3,7,11-trien-2-9-one,(3E,7E)-2a,5a,10b,13a-tetraacetoxy-20-hydroxy-3,8-secotaxa-3,7,11-trien-9-one, and(3E,7E)-10b,13a-diacetoxy-2a,5a,20-trihydroxy-3,8-secotaxa-3,7,11-trien-9-one on silica gel with hexane - acetone,hexane - ethyl acetate, and chloroform - methanol. Also analytical TLC; detection under UV and by spraying with 10%sulfuric acid followed by heating.

15a

88 070 Y.-P. SHI, W. GUO, Z.-J. JIA* (*Dept. of Chem., Nat. Lab. of Appl. Org. Chem., Lanzhou Univ., Lanzhou 730000, P.R.China): Germacranolides from Carpesium lipskyi. Planta med. 65, 94-96 (1999). Preparative TLC of b-sitosteroland 2 new sesquiterpene lactones, the pyrazoline derivatives of carpelipine A and B, on silica gel with petroleum ether -dichloromethane - ethyl acetate 20:3:1 for carpelipine A and petroleum ether - ethyl acetate 6:1; 1 or 2 developmentsfor carpelipine B.

15a, 8b

17. Amines, amides and related nitrogen compounds

88 071 M.S. NARVEKAR, A.K. SRIVASTAVA*, (*Dept. of Chem., Univ. of Mumbay, Vidyanagari, Santacruz (E), Mumbai 400098, India): HPTLC separation and determination of banned amine isomers, with reference to the German banon azo dyes. J. Planar Chromatogr. 14, 360-363 (2001). HPTLC of 7 amines (p-chloroaniline, o-toluidine, o-anisidine,2-naphthylamine, 4-chloro-o-toluidine, 2-diaminotoluene, 2-aminio-4-nitrotoluene) on silica gel plates withdichloromethane (developing distance 4.5 cm), drying and development with toluene - tetrahydrofuran 10:1 to a distanceof 8.5 cm. Visualization under UV 254 nm. New quantitative very simple, accurate, low-cost, rapid and highly selectivemethod.

17a, 30a



18. Amino acids and peptides, chemical structure of proteins

88 072 P.G. BESANT*, M.V. LASKER, C.D. BUI, C.W. TURCK, (*Dept. of Med., Univ. of California San Francisco, SanFrancisco, California 94143-0724, [email protected]): Phosphohistidine analysis using reversed-phasethin-layer chromatography. Anal. Biochem. 282, 149-153 (2000). TLC of phosphorylated amino acids(phospholysine, phosphoserine, phosphothreonine, phosphoarginine, phosphotyrosine, phosphohistidine) on RP-18 withethanol - NH3 conc. - water 65:10:16. Detection by spraying with 0.2% ninhydrin in ethanol and heating at 37°C for 30min.

18, 32

19. Proteins

88 073 Q. FU (Fu Qiang)*, B. LIU (Liu Bingwen), (*Apolipoproteins Res. Unit., Inst. Biochen. & Mol. Biol., West China Univ.Med. Sci, Chengdu 610041, P.R. China): (Hypochlorite und Cu2+ induced oxidation of high density lipoprotein. )(Chinese). Chinese J. Biochem. & Biophys. (Shengwu Huaxue Yu Shengwu Wuli Xiebao) 33 (3), 262-268 (2001). TLCof high density lipoprotein and oxidized high density lipoprotein on silica gel with chloroform - NH3 - methanol - water180:11:108:11. Detection by exposure to iodine vapor. Also SDS-PAGE on 10% polyamide at 15 V/cm for 5 hours.Investigation of the properties of oxidatively modified high density lipoprotein, the effects of hypochlorite and Cu2+ onHDL in vitro.

19, 4d

22. Alkaloids

88 074 L. ASTUDILLO, K. J‹RGENS, G. SCHMEDA-HIRSCHMANN*, G.A. GRIFFITH, D.J. HOLT, P.R. JENKINS, (*Instituto deQuÌmica de Recursos Naturales, Universidada de Talca, Casilla 747, Talca, Chile): DNA binding alkaloids fromProsopis alb. Planta med. 65, 161-162 (1999). Analytical and preparative TLC of b-phenethylamine the new alkaloidand 2-b-methyl-3-b-hydroxy-6-b-piperidinedodecanol on silica gel with chloroform - methanol 9:1+ 1% NH3. Detectionwith Dragendorff reagent.

22

88 075 B. BAGHDIKIAN, H. GUIRAUD-DAURIAC, E. OLLIVIER*, A. N'GUYEN, G. DUMUEUIL, G. BALMSARD, (*Lab. ofPharmacogn., Fac. of Pharm., 27 Boulevard Jean Moulin, F-13385 Marseille cedex 5, France): Formation of nitrogencontaining metabolites from the main iridoids of Harpagophytum procumbens and Harpagophytum zeyheri byhuman intestinal bacteria. Planta med. 65, 164-166 (1999). TLC of aucubin B, harpagide and harpagoside on silicagel with ethyl acetate - methanol 4:1. Detection by spraying with a solution of anisaldehyde - sulfuric acid - acetic acid1:2:100 followed by heating.

22

88 076 P.J. HOUGHTON*, T.Z. WOLDEMARIAM, Y. WATANABE, M. YATES, (*Pharmacogn. Res. Lab., Dept. of Pharm.,King's Coll. London, Manresa Road, London SWB 6KX, London): Activity against mycobacterium tuberculosis ofalkaloid constituents of Angostura bark, Galipea officinalis. Planta med. 65, 250-254 (1999). Fractionation usingcentrifugal TLC on silica gel plates; analytical gradient elution of 300 mL aliquots of n-hexane - ethyl acetate 9:1 tohexane - ethyl acetate 1:1 and final stripping of the layer with methanol. TLC of allocuspareine, cuspareine,4-methoxy-2-n-pentyl-quinoline, cusparine, galipine, N-methyl-2-quinolone on silica gel with n-hexane - ethyl acetate -butanone 5:5:2. Visualization by spraying with Dragendorff's reagent. Also preparative TLC with n-hexane - ethylacetate 9:1.

22

88 077 K.K. LEE, B.-N. ZHOU, D.G.I. KINGSTON*, A.J. VAISBERG, G.B. HAMMOND, (*Dept. of Chem., Virginia Polytech andState Univ., Blacksburg, Virginia 24061-0212, USA): Bioactive indole alkaloids from the bark of Uncariaguianensis. Planta med. 65, 759-760 (1999). Preparative TLC of uncarine C and E on RP-18 with methanol - water3:1.

22, 23c

88 078 S.V.K. MOIDEEN, P.J. HOUGHTON*, P. ROCK, S.L. CRAFT, F. ABOAGUE-NYAME, (Pharmacogn. Res. Lab., Dept. of



88 078 S.V.K. MOIDEEN, P.J. HOUGHTON*, P. ROCK, S.L. CRAFT, F. ABOAGUE-NYAME, (Pharmacogn. Res. Lab., Dept. ofPharm., King's Coll. London, Manresa Road, London SW3 6LX, U.K.): Activity of extracts and naphthoquinonesfrom Kigelia pinnata against Trypanosoma brucei and Trypanosoma brucei rhodesiense. Planta med. 65,536-540 (1999). Analytical and preparative TLC of the new compounds2-(1-hydroxyethyl)-naphtho[2,5-b]furan-4,9-quinone, isopinnatol, kigelinol, isokigelinol on silica gel with chloroform -ethyl acetate 4:1, petroleum ether 40-60°C - chloroform - methanol 16:24:1, toluene - ethyl acetate 5:2, petroleum ether40-60°C - ethyl acetate 1:1 and 2:1, chloroform - ethyl methyl ketone - methanol 10:6:1 and chloroform isopropanol19:1. Detection under UV 254 and 366 nm and daylight after spraying with 0.5% anisaldehyde in methanol - sulfuricacid - acetic acid 17:1:2 and heating at 105°C for 10 min.

22

88 079 N. KEAWPRADUB, E. ENO-AMOOQUAYE, P.J. BURKE, P.J. HOUGHTON*, (*Pharmacogn. Res. Lab., Dept. ofPharm., King's Coll. London, Manresa Road, London SW3 6LX, UK): Cytotoxic activity of indole alkaloids fromAlstonia macrophylla. Planta med. 65, 311-315 (1999). TLC of O-acetylmacralstonine on silica gel with chloroform-methanol 14:1. Detection under UV.

22

88 080 H. OHTSU, R. TANAKA*, S. MATSUNAGA, H. TOKUDA, H. NISHINO, (*Dept. of Med. Chem., Osaka Univ. of Pharm.Sci. , 4-20-1 Nasahora, Takatsuki, Osaka 569-1094, Japan): Anti-tumor-promoting rearranged abietane diterpenesfrom the leaves of Ladix kaempferi. Planta med. 65, 664 (1999). Anal. and preparative TLC of e.g.9,10a-epoxy-9,10-seco-abieta-8,11,13-trien-18-oic acid and 9a, 13a-epoxy-8-oxo-9(8-7)abeo-7bH-abietan-18-oic acidon silica gel with chloroform - methanol 100:1. Detection under UV 254 nm.

22, 15a

88 081 N.M. PHUONG, T.V. SUNG, A. PARZEL, J. SCHMIDT, G. ADAM*, (*Inst. of Plant Biochem., Postfach 110432,DE-06018 Halle/Saale, Germany): Two new b-carboline alkaloids from Hedyotis capitellata var. mollis. Plantamed. 65, 761-762 (1999). Preparative TLC of hedyocapitelline and hedyocapitine on silica gel with cyclohexane -chloroform - diethylamine 4:16:1.

22

88 082 X. QIN (Qin Xuegong)*, Y. YUAN (Yuan Yingjin), (*Chem. Eng. School, Tianjin Univ., Tianjin 300072. P.R. China): (Study of the extraction of alkaloids in seed of Sophora alopecuroides. ) (Chinese). J. Chinese Trad. & Herb.Drugs (Zhongcaoyao) 32 (7), 604-606 (2001). TLC on silica gel with benzene - acetone - methanol 9:3:2. Detection byspraying with 5% solution of potassium iodobismuthate. Quantitation of total alkaloids by densitometry at 489 nm. Useof the procedure in the investigation of the extraction conditions.

22, 32e

88 083 A. SARI, (Fac. of Pharm., Dept. of Pharmacognosy, Istanbul Univ., TR-34452 Istanbul, Turkey): Alkaloids fromGlaucium leiocarpum. Planta med. 65, 492 (1999). Preparative TLC of (+)-glaucine, 6,6a-dehydronorglaucine,oxoglaucine, (+)-lastourvilline, (+)-predicentrine, (+)-dihydropontevedrine, secoglaucine, (-)-N-methylcoclaurine,protopine, allocryptopine on silica gel with benzene - acetone - ether - NH3 40:60:10:3, cyclohexane - chloroform -diethylamine 7:2:1 and toluene - acetone - ethanol -; analytical TLC of (+)-N-methylglaucine on silica gel with chloroform- methanol 4:1.

22

88 084 Y. SCHOLL, N. ASANO, B. DRÄGER*, (*Martin-Luther-Univ. Halle-Wittenberg, Inst. Pharm. Bio., Hoher Weg 8,DE-06120 Halle/Saale, Germany): Automated multiple development thin-layer chromatography for calysteginesand their biosynthetic precursors. J. Chromatogr. A 928 (2), 217-224 (2001). HPTLC-AMD of calystegines andtropanes on silica gel with an 11-step gradient from methanol to chloroform with basic preconditioning with 9 Mammonia or a 15-step gradient from ethanol to dichloromethane with basic preconditioning with 14 M ammonia.Detection by dipping the lower half of the plate in silver nitrate - sodium hydroxide reagent to visualize calystegines andby spraying the upper half of the plate with chlorine-tolidine reagent or alternatively, for detection of tropanes, withDragendorff var. Munier reagent.

22

88 085 J.-J. SHEN, C.-Y. DUH, I.-S. CHEN*; (Graduate Inst. of Pharm. Sci. , Kaohsiung Med. College, Kaohsiung, Taiwan, P.R.China): New tetrahydroprotoberberine N-oxide alkaloids and cytotoxic constituents of Corydalis tashira. Plantamed. 65, 643-647 (1999). TLC of e.g. (-)-cis-corydalmine N-oxide and (-)-trans-corydalmine N-oxide on silica gel withchloroform - ethanol 5:2, of (-)-trans-isocorylpalmine N-oxide with acetone - methanol, of norsanguinarine anddihydrosanguinarine with n-hexane - ethyl acetate 4:1, of oxysanguinarine with benzene - methanol 15:1, of(-)-tetrahydropalmatine with chloroform - ethylacetate 7:1, of (-)-corydalmine and(-)-scoulevine with chloroform - acetone4:1, and of palmatine with chloroform - methanol 3:1. Detection under UV 254 nm.

22



88 086 B. SZABO*, L. BOTZ, A. LAKATOS, T. KÖSZEGI, (Pécs Univ., Fac. of Sci., Dept. of Botany, H-7624 Pécs, Ifjusag u. 6,Hungary): HPTLC and HPLC separation of poppy alkaloids under induced plant stress. Proc. Intern. Symp. onPlanar Separations, Planar Chromatography 2001, pp. 181-190. TLC and HPTLC of extracts of Papaver somniferumand codeine, narceine, morphine, thebaine, papaverine and narcotine as standards on silica gel with toluene - acetone- ethanol - 25% NH3 20:20:3:1. Detection with formaldehyde - sulfuric acid reagent (Marquis' Reagent). Quantitation bydensitometry at 600 nm. Simple TLC method.

22

88 087 N. ÜNVER, S. NOYAN, T, GÖTZLER, M.A. ÖNÜR, B. GÖTZLER, M. HESSE*, (*Inst. of Organic Chem., Univ. of Zürich,Winterthurerstr. 190, CH-8057 Zürich, Switzerland): Three new tazettine-type alkaloids from Galanthus gracilis andGalanthus plicatus subsp. byzantinus. Planta med. 65, 347-350 (1999). Preparative TLC of (+)-isotazettinol{(3R,4aS,6aS,12bS)-5-methyl- 4,4a,5,6-tetrahydro-3H,8H-[1,3]dioxolo[6,7][2]benzopyrano[3,4-c]indole-3,6a-diol} onsilica gel with benzene - chloroform - methanol (saturated with ammonia vapor) 7:2:1, (+)-3-O-demethylmacronine{(3R,4aS,6aR,12bS)-3-hydroxy-5-methyl-3,4,4a,5,6,6a-hexahydro- [1,3]dioxolo[6,7][2]benzopyrano[3,4-c]indole-8-one}with toluene - acetonitrile (saturated with ammonia vapor) 7:3 and (+)-3-O-demethyl-3-epimacronine{(3S,4aS,6aR,12bS)-3-hydroxy-5- methyl-3,4,4a,5,6,6a-hexahydro-[1,3]dioxolo[6,7][2]-benzopyrano[3,4]-c]indole-8-one}with benzene - ethyl acetate - ethanol (saturated with ammonia vapor) 16:3:1.

22

88 088 M. WAKSMUNDZKA-HAJNOS*, A. PETRUCZNIK, (*Dept. of Inorg. and Anal. Chem., Med. Univ., Staszica 6, 20-081Lublin, Poland): Retention behavior of some alkaloids in thin layer chromatography with bonded stationaryphases and binary model phases. J. Planar Chromatogr. 14, 364-370 (2001). TLC of 24 alkaloids (aconitine -yohimbine) on silica gel and aminopropyl-modified silica gel plates as well as on cyanopropyl-, diol- and RP18-modified silica gel plates using a variety of binary mobile phases prepared from n-hexane and polar modifiers -2-propanol, ethyl acetate, methyl ethyl ketone, tetrahydrofuran, dioxane - in different concentrations for NPchromatography and reversed-phase systems with buffered aqueous -acetonitrile mobile phases (pH 9.15).Visualization at 254 nm.

22

28. Antibiotics, Mycotoxins

88 089 S. NAGY*, B. KOCSIS, T. KÖSZEGI, L. BOTZ, (*Pécs Univ., Fac. of General Medicine, First Internal Med., H-7624Pécs, Ifjjusag ut 13, Hungary): Optimal life condition of test bacteria for direct bioautographic detection. Proc.Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp. 173-179. Bioautography of cefazolin on silicagel TLC and HPTLC plates (without development) by dipping the plates into bacteria suspension or on the plate.Visualization of the antimicrobial activity by use of MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide).The method was considered as suitable for determination of the viability (capable of living) of given test bacteria on theTLC plate.

28a

88 090 J. NOWAKOWSKA*, J. HALKIEWICZ, J. W. LUKASIAK, (*Med. Univ. of Gdansk, Fac. of Pharm., Hallera 107, PL80-416, Gdansk, Poland): The retention behavior of selected macrocyclic antibiotics on polyamide TLC plates. J. Planar Chromatogr. 14, 350-354 (2001). TLC of troleandomycin, tylosin, rifamycin B, vancomycin, erythromycin, andrifampicin on polyamide 11 plates with five binary solvent mixtures methanol - water, ethanol - water, propanol - water,acetonitrile - water, and tetrahydrofuran - water. Detection by spraying with anisaldehyde - methanol - acetic acid -orthophosphoric acid - sulfuric acid 1:100:10:10:5 and heating at 110°C for 10 min.

28a

88 091 J. SKARKOVA, V. OSTRY, J. RUPRICH, (Nat. Inst. of Public Health Prague, Centre for Hygiene of Food Chains, Nat.Ref. Lab. for Microscopic Fungi and their Toxins, Palavkeho 1-3, 612 42 Brno. The Czech Republic): Application ofplanar chromatography on determination of mycotoxins in the Czech Republic. Proc. Intern. Symp. on PlanarSeparations, Planar Chromatography 2001, pp. 343-349. HPTLC of aflatoxins (B1, B2, G1, G2, M1), ochratoxin A,patulin, deoxynivalenol, fumonisins, sterigmatocystin, cyclopiazonic acid and altenuene on silica gel with chloroform -acetone 22:3, benzene - methanol - acetic acid 18:1:1, toluene - ethyl acetate - formic acid 5:4:1, chloroform - acetone -2-propanol 8:1:1, chloroform - methanol - water - acetic acid 55:36:8:1, acetonitrile - 2-propanol - 0.25 M phosphoric acid4:5:10, ethyl acetate - 2-propanol - NH3 6:3:2 and toluene - ethyl acetate - formic acid 6:3:1, respectively. Detectionunder UV or by spraying with aluminium chloride, 4-methoxybenzaldehyde, trifluoroacetic acid - aluminium chloride, and4-dimethylaminobenzaldehyde. Quantitation by densitometry at different wavelengths in absorbance or fluorescencemode.

28b



29. Pesticides and other agrochemicals

88 092 A. MOHAMMAD*, I.A. KHAN, N. JABEEN, (*Anal. Lab., Dept. of Appl. Chem., Fac. of Eng., Aligarh Muslim Univ.,Aligarh 202002, India): Thin-layer chromatographic studies of the mobility of pesticides through soil-containingstatic flat-beds. J. Planar Chromatogr. 14, 283-290 (2001). TLC of chlorpyrifos, cypermethrin, dimethoate,endosulfan, fenvalerate, and phosphamidon on silica gel, soil and mixed layers with aqueous ammonium or sodium saltsolutions, with or without added N-cetyl-N,N,N-trimethylammonium bromide (CTAB), with pure organic solvents, andwith aqueous CTAB systems.

29

88 093 D.E. WEDGE*, D.G. NAGLE, (*USDA-ARS, Nat. Prod. Utilization Res. Unit, Natl. Center for Nat. Prod., Univ. ofMississippi, University, Mississippi 38677, [email protected].): A new 2D-TLC bioautography method for thediscovery of novel antifungal agents to control plant pathogens. J. Nat. Prod. 63, 1050-1054 (2000). 2D-TLC offungicides or of crude extract material on silica with methanol - dichloromethane 1:9 in the first dimension, and, after airdrying with ethyl acetate - hexane 1:1 in the second direction. Visualization under UV 254 nm or with H2SO4 in ethanol1:19 and heating. Spraying of the dried plates with a nutrient broth spore suspension and incubation during 4 days.Determination of the dimensions of the zones of antifungal activity.

29e

30. Synthetic and natural dyes

88 094 M. LIGOR, B. BUSZEWSKI*, (*Dept. of Environmental Chem. and Ecoanalytics, Fac. of Chem., Nicholas CopernicusUniv., 7 Gagarin St, 87100 Torun, Poland): Application of TLC and OPLC in the determination of pigments fromnatural products. J. Planar Chromatogr. 14, 334-337 (2001). HPTLC (radial or one-dimensional development) andOPLC of anthocyanins and chlorophyll on silica gel plates with 8 different proportions of 2-propanol - acetone - water.

30b

88 095 N.U. PERISIC-JANJIC*, B.L. VUJICIC, N. DIMIC, (*Dept. for Talents, Dr. Ilije Duricica 3, 21000 Novi Sad, Yugoslavia): Separation of red paprika color components by use of thin-layer chromatography on a variety of supports. J.Planar Chromatogr. 14, 391-393 (2001). TLC and HPTLC of red paprika color components (e.g. b-carotene,canthaxanthine) on silica gel plates, and on aminoplast (carbamideformaldehyde polymer) with petroleum ether -acetone 17:3 and n-hexane - acetone 4:1. Very successful separation on Lichrospher Si 60 HPTLC plates; the colorcomponents were, in particular, stable for one month - on normal silica gel and aminoplast for one day only. Sensitiveand specific method.

30b

32. Pharmaceutical and biomedical applications

88 096 D. AGBABA*, Z. MILOJEVIC, S. ERIC, G. MARKOVIC, M. SOLUJIC, M. ALEKSIC, (Dept. of Pharm. Chem. and DrugAnal., Fac. of Pharm., P.O.B. 146, 11221 Belgrade, Serbia, Yugoslavia): The determination of dexamethasone andxylometazoline in nasal drops with different preservatives by high-performance thin layer chromatography. Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp. 165-171. HPTLC of, xylometazolinehydrochloride and methyl p-hydroxybenzoate on silica gel with ethyl acetate - acetonitrile - diethylamine - water 3:3:1:1.Densitometry at 240 nm in reflectance/absorbance mode. Validation parameters, such as linearity, precision, accuracyand LOQ (limit of quantification) were determined and found to be satisfactory. Simple and reliable method.

32a

88 097 B. BAGOCSI, T. V÷R÷S, M. MEZEI, Z. VEGH, K. FERENCZI-FODOR, (Richter Gedeon Ltd., Budapest, Hungary): Fastand economical purity testing of nestorone drug substance by OPLC. Proc. Intern. Symp. on Planar Separations,Planar Chromatography 2001, pp. 211-215. OPLC of nestorone and 7 similar compounds on HPTLC silica gel withcyclohexane - ethyl acetate - chloroform 42:29:29. Visualization after spraying with sulfuric acid and under UV 365 nm.

32a

88 098 W. BAI (Bai Wulong)*, SH. QUAN (Quan Shan), F. ZHAO (Zhao Fengming), B. BAO (Bao Baoquan), Q. CHEN (ChenQuiglian), Tonglaga, (*Tangliao Municip. Inst. Drug Cont., Tongliao, Inner Mongolia, 028000, P.R. China): (Identification of Gordenia jasminoides Ellis and Gordenia jasminoides Ellis. F. longicarpa Z.W. Xie et Okada.)(Chinese). J. Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32 (8), 751-752 (2001). TLC on silica gel withtrichloromethane - methanol 3:1. Detection by spraying with 50% sulfuric acid in ethanol and heating at 1108C for 10min, and under UV 365 nm. Identification by finger print technique combined with UV spectra comparison.

32e



88 099 Anne BLATTER*, E. REICH (*Dept. of Pharm., Univ. of Basel, CH-4051 Basel, Switzerland): Recent investigationson St John's Wort by HPTLC. Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp. 23-32.Qualitative and quantitative HPTLC of hypericin, pseudohypericin, rutin, chlorogenic acid, hyperoside, quercetin,hyperforin, quercitrin and biapigenin on silica gel and silica gel diol. Fingerprint analysis was carried out on silica gelwith 9 different solvent systems. Stability test of hypericin on silica gel with ethyl acetate - dichloromethane - acetic acid- formic acid - water 100:25:10:10:11. Visualization of hypericin and pseudohypericin under UV 366 nm. Quantitation bydensitometry in fluorescence mode at 366 nm on silica gel diol. Videodocumentation.

32e, g

88 100 X. CAO (Cao Xiaofeng)*, SH. ZHANG (Zhang Shengwan), Y. LI (Li Yonghong), J. LIN (Lin Jiancheng), J. TONG (TongJianbo), (Shanxi Med. Univ., Taiyuan 030001, P.R. China): (Determination of 4-chloro-3,5-dimethyl phenol inantiseptics and synthetic products by thin-layer chromatography. ) Chinese J. Chromatogr. (Sepu) 19 (4),356-357 (2001). TLC on silica gel with benzene - chloroform 2:3. Detection under UV 254 nm. Spectra was recorded toidentify the spot and determine the maximum absorbance wavelength for densitometry.

32c

88 101 S.K. CHAUHAN*, B.P. SINGH, S. AGARWAL, (*R.&D Centre, Indian Herbs Saharanpu-247001, UP, India): Standardisation of gardenia gummifera linn with reference to gardenin by HPTLC. Indian Drugs 38 (8), 418-420(2001). HPTLC on silica gel with chloroform -methanol 24:1. Quantitation by densitometry at 260 nm.

32e

88 102 X. CHE (Che Xiaoyan), (Sichuan Provin. Inst. Drug Cont., Chengdu 610036, P.R. China): (Determination oftanshinone IIA in Jinbuhuan pills by thin-layer chromatography. ) (Chinese). J. Chinese Trad. & Herb. Drugs(Zhongcaoyao) 32 (9), 802-803 (2001). TLC on silica gel with benzene - ethyl acetate 19:1. Detection under daylight.Quantitation by fluoro densitometry at 465 nm.

32c

88 103 H. CHEN (Chen Huangbao), (Zhanjiang Municip. Inst. Drug Cont., Zhanjiang, , Guandong 524037, P.R. China): (Pharmacogniostic studies on Tianniundali, Radix Millettia speciosa, and Kuniudali, Radix Millettia championi. )(Chinese). J. Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32 (9), 843-845 (2001). TLC on silica gel with chloroform -diethylamine - methanol 45:1:5. Detection under UV and by spraying with 1% 4-dimethylaminobenzaldehyde in ethanoland 20% hydrochloric acid solution. Identification by finger print technique combined with UV spectra.

32e

88 104 R. CHEN (Chen Ruifen)*, L. ZHU (Zhu LAN), CH. GUI (Gui Cheng), J. SHI (Shi Jianping), (*Shanghai 1st People'sHosp. 200080 Shanghai, P.R. China): (Identification of Huangban I granules. ) (Chinese). J. Chin. Trad. Patent Med.(Zhongchengyao) 23 (11), 851-853 (2001). TLC on silica gel with 1) ethanol - 0.5% HCl 8:2, 2) petroleum ether(60-90°C) - ethyl acetate 20:1. Detection 1) by exposure to ammonia vapor and under UV 254 nm, 2) by spraying with5% p-dimethylaminobenzaldehyde in 10% sulfuric acid and heating. Identification by finger print technique.

32c

88 105 B. CHI (Chi Baochan)*, F. HOU (Hou Feng), M. LI (Li Mei), (*Liaoning Provin. Inst. Drug Cont., Senyang 110023, P.R.China): (Determination of corydalis B in Qizhi Weitong tablets by thin-layer chromatography. ) (Chinese). J.Chin. Trad. Patent Med. (Zhongchengyao) 23 (11), 797-798 (2001). TLC on silica gel with hexane - chloroform -methanol 10:6:1. Detection by exposure to iodine vapor. Quantitation by densitometry at 430 nm.

32c

88 106 J. CUI (Cui Jian)*, H. GENG (Geng Hui), S. LIN (Lin Sujie), X. CHEN (Chen Xin), (*Changchun Coll. CTM, Changchun130021, P.R. China): (Study of the chemical constituents of sophora tonkinensis Gagnep in its compoundformulates. ) (Chinese). J. Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32 (7), 613-614 (2001). TLC on silica gelwith toluene - butanone - 2-isopropanol - NH3 20:20:3:1. Detection by spraying with a solution of potassiumiodobismuthate. Identification by finger print technique. Quantitation of matrine and oxymatrine by densitometry at 510nm.

32c

88 107 J. DENG (Deng Junli)*, A. ZOU (Zou Ailing), (Yantai Munic. Inst. Drug Cont., Yantai 264000, P.R. China): (Analysis ofXiaohuolo pills by thin-layer chromatography. ) (Chinese). Chinese J. Hosp. Pharm. (Zhongguo Yaoxue Zazhi) 21(5), 312-313 (2001). TLC on silica gel with 1) benzene - ethyl acetate 9:1, 2) hexane - ethyl acetate - ethanol 2:18:5, 3)petroleum ether (60-90°C) - ethyl acetate 17:1. Detection 1) by spraying with 10% sulfuric acid in ethanol and heating at105°C for 5 min, 2) by exposure to iodine vapor, 3) by spraying with 5% vanillin-sulfuric acid reagent and heating at105°C for 5 min. Identification by finger print technique.

32c

88 108 X. DONG (Dong Xiaoping)*, W. LIN (Lin Wuxia), H. HIANG (Jiang Haoxiang), J. LIN (Lin Jianfang), (*Jiangsu Provin.



88 108 X. DONG (Dong Xiaoping)*, W. LIN (Lin Wuxia), H. HIANG (Jiang Haoxiang), J. LIN (Lin Jianfang), (*Jiangsu Provin.Inst. Drug Cont., Nanjing 210008, P.R. China): (Determination of arginine in Radix isatidis by thin-layerchromatography. ) (Chinese). Chin. J. Anal. Lab. (Fenxi Shiyanshi) 20 (4), 92-93 (2001). TLC on silica gel withpropanol - methanol - NH3 5:1:4. Detection by spraying with 5 g/L ninhydrin in ethanol. Quantitation by densitometry at560 nm.

32c

88 109 I. FECKA*, W. CISOWSKI, M. LUSKIEWICZ, (Dept. of Pharmacogn., Med. Univ. of Wroclaw, Nankiera 1, 50-149Wroclaw, Poland): TLC determination of catechin and epicatechin in an extract from Uncaria tomentosa bark bychemically modified stationary phases. Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001,pp. 201-209. TLC and HPTLC of plant extracts and catechin and epicatechin as standards on cellulose, silica gel, andcyano-, amino- and RP-18 modified silica gel with 8 different mobile phases. The best separation of catechin andepicatechin was achieved on the aminopropyl and octadecyl plates using acetone - acetic acid 93:7 and water -methanol - formic acid 84:15:1 or 69:30:1, respectively. Visualization by spraying with vanillin in sulfuric acid or bycoupling with bis-diazotized sulfanilamide.

32e

88 110 SH. FENG (Feng Sheng)*, H. LI (Li Huixia), (*Guangzhou the 1st Fac. TCM, Guangzhou 510140, P.R. China): (Determination of ecdultin in Huanchun Buli pills by thin-layer chromatography. ) (Chinese). J. Chinese Trad. &Herb. Drugs (Zhongcaoyao) 32 (9), 803-804 (2001). TLC on silica gel with benzene - ethyl acetate 30:1. Detectionunder UV 365 nm. Quantitation by densitometry at 325 nm.

32c

88 111 N. GALAND, J. POTHIER, C. VIEL, (Lab. of Pharmacogn., Fac. of Pharm., 31 avenue Monge, 37200 Tours, France): OPLC and AMD, recent techniques of planar chromatography - their interest for separation and characterizationof extractive compounds. Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp. 313-320.OPLC of natural phenolic compounds (coumarins, furanochromones, flavonoids, anthocyanins) with ethyl acetate -chloroform 3:2; 9:1 and ethyl acetate - MEK - formic acid - 2 M HCl 65:10:6:9. Detection under UV 365 nm for coumarinsand furanochromones; spraying with 1% methanolic diphenylboric acid b-ethylamino ester followed by 5 p. 100ethanolic polyethyleneglycol 400, then observation at 365 nm for flavonoids and by direct visual observation foranthocyanins. Alkaloids were separated by semipreparative OPLC on aluminium oxide with hexane - ethyl acetate 1:1,ethyl acetate and ethyl acetate saturated with 28% NH3. Detection under UV 365 nm, or after spraying withDragendorff's or iodoplatinate reagent. Quantitation by densitometry at 540 nm. AMD for Opium alkaloids on silica gelwith methanol 100, methanol/acetone 50/50 v/v, dichloromethane 100, ethyl acetate, ethyl acetate/dichloromethane;visualization with potassium iodoplatinate.

32d, e

88 112 P. GU (Gu Ping)*, Y. LI (Li Yuru), Y. WU (Wu Yuxia), S. JIANG (Jiang Sumei), (*Lianyungang Haoshen, Med. GroupCo. Ltd., Lianyungang 222002, P.R. China): (Analysis of lefrozole and its tablets by thin-layer chromatography. )(Chinese). Chinese J. Pharm. Ind. (Zhongguo Yiyao Gongye Zazhi) 32 (7), 317-318 (2001). TLC of letrozole on silicagel with ethyl acetate - acetone 2:1 and traces of NH3. Detection under UV 254 nm. Identification of the purity of themedicine by comparison with the standard. Comparison with HPLC.

32c

88 113 Y. GU (Gu Yu)*, Y. FENG (Feng Yufei), W. JIANG (Jiang Wenqing), D. ZOU (Zhou Ding), (*Bejing Hosp., MinistryHealth PRC, Beijing 100730, P.R. China): (Determination of valproates in serum by thin-layer chromatographyafter derivtization. ) (Chinese). Chinese J. Hosp. Pharm. (Zhongguo Yaoxue Zazhi) 21 (8), 475-477 (2001). TLC onsilica gel with hexane - isopropyl ether 4:1. Detection under UV. Quantitation by densitometry at 240 nm.

32c

88 114 B. GUAN (Guan Bing)*, X. DANG (Dang Xuemei), (*Suihua Municip. Inst. Drug Cont., Suihua Helongjiang 152062, P.R.China): (Proposal of a procedure for the identification of Kanfu Xiaoyan suppository. ) (Chinese). J. ChineseTrad. & Herb. Drugs (Zhongcaoyao) 32 (6), 517-518 (2001). TLC on silica gel with isooctane - ether - acetic acid -butanol - water 10:5:5:3:1. Detection by spraying with 10% sulfuric acid in ethanol and heating, and under UV 365 nm.Identification of hyodeoxycholic acid by comparison with the standard.

32c

88 115 S. GUI (Gui Sulan)*, A GENG (Geng Aiping), Y. LIN (Lin Yongzhong), (*Changzhi Inst. Drug Cont., Changzhi, Shanxi



88 115 S. GUI (Gui Sulan)*, A GENG (Geng Aiping), Y. LIN (Lin Yongzhong), (*Changzhi Inst. Drug Cont., Changzhi, Shanxi046011, P.R. China): (Identification of Guifudihuang pills by thin-layer chromatography. ) (Chinese). J. Chin. Trad.Patent Med. (Zhongchengyao) 23 (11), 837-838 (2001). TLC on silica gel with 1) cyclohexane - ethyl acetate 3:1, 2)petroleum ether (30-60°C) - ethyl acetate 17:3. Detection 1) by spraying with 5% FeCl3 in ethanol and heating, 2) byspraying with 0.1% 2,4-dinitrophenylhydrazine in ethanol. Identification by finger print technique. Discussion of use ofthe procedure for the quality control of the medicine.

32c

88 116 R.D. HANDRAL*, M.M. RAVISHANKARA, S.A. SHAH, (*L.M. Col. of Pharm, Ahmedabad - 380009, India): Estimationof nicotine from Gutka, a chewable tobacco preparation by new HPTLC method. Indian J. Pharm. Sci. 63 (3),250-253 (2001). HPTLC of nicotine on silica gel with chloroform - methanol - NH3 6:5:1. Densitometry at 255 nm.Different analytical parameters such as accuracy, precision, linearity, LOD, LOQ were established. Comparison with UVspectrophotometric method.

32

88 117 Y. HUANG (Huang Yanmiu)*, L. PAN (Pan Linhua), P. GUAN (Guang Peng), G. LI (Li Guangzhen), W. XU (XuWennian), X. YU (Yu Xiaojing), (*456th Hosp. PLA, Jinan 250031, P.R. China): (Determination ofprotocatechualdehyde in the Donhong injection by thin-layer chromatography. ) (Chinese). Chinese J. Hosp.Pharm. (Zhongguo Yaoxue Zazhi) 21 (10), 605-607 (2001). TLC on silica gel with chloroform - acetone -formic acid16:2:1. Detection by spraying with phloroglucinol reagent. Identification by comparison with the standard. Quantitationby densitometry at 495 nm.

32c

88 118 M.A. JADHAV*, P.M. MELLO, C.I. Jolly, (*Dept. of Pharmacogn. & Phytochem., Prin. K.M. Kundnani Col. of Pharm.,Worli, Mumbai-400018, India): Fluorescence quenching as a tool for quality control of crude drugs. Indian Drugs38 (3), 147-149 (2001). Spotwise application of 20 µL methanolic plant extracts of different concentrations on silica gelfor fluorescence quenching under UV 254 nm. Quenching which was directly proportional to the concentration of eachplant extract was used as aid for routine quality control of crude drugs.

32

88 119 H. JIANG (Jiang Huizheng)*, A. YIN (Yin Aiquin), SH. DING (Ding Shiyang), (*Yantai Municp. Inst. Drug Cont., Yantai264000, P.R. China): (Identification of Aristolochia fangchi Y. C. Wu ex L.D. Chou et S.M Huvang and Aristolochiatagala chamq.) (Chinese). Chinese J. Hosp. Pharm. (Zhongguo Yaoxue Zazhi) 21 (9), 570-571 (2001). TLC on silicagel with benzene - acetone - formic acid 32:8:1. Detection under UV 365 nm. Identification by finger print techniquecombined with UV spectra comparison.

32e

88 120 P. KASTNER*, J. KLIMES, G. ZIMOVA, V. KLIMESOVA, (*Charles Univ. in Prague, Fac. of Pharm. in Hradec Kralove,Heyrovskeho 1203, 500 05 Hradec Kralove, Czech Republic): Reversed-phase thin-layer chromatographicdetermination of the lipophilicity of potential antituberculotic compounds. J. Planar Chromatogr. 14, 291-295(2001). TLC of 36 4-benzylthio derivatives of 2-pyridinecarbothioamide and 2-pyridinecarbonitrile on silanized silica gel(impregnation by overnight (15.5 h) development with diethyl ether - silicone oil 19:1) with phosphate buffer of pH 7.4 or3.0 or 5% ammonia as aqueous component of the mobile phase and acetone or methanol as organic modifier.Visualization at 254 nm.

32a, 23d

88 121 A. KOWALCZYK*, G. MATYSIK, H. RZADKOWSKA-BODALSKA, W. CISOWSKI, (*Dept. of Pharmacogn., WroclawMed. Univ., Nankiera 1, 50-140 Wroclaw, Poland): Thin-layer chromatography and densitometry of caffeic acid insome Dipsacaceae family plants. J. Planar Chromatogr. 14, 175-177 (2001). HPTLC of caffeic acid (before and afteracid hydrolysis) on silica gel with a mixture of toluene, hexane, ethyl acetate, and methanol as mobile phases.Development 'face down' in a horizontal chamber; visualization under UV 254 and 366 nm. Quantitative determinationsby videodensitometry.

32e

88 122 J. KRZEK*, M. DABROWSKA, U. HUBICKA, (*Dept. of Inorg. and Anal. Chem., Coll. Medicum of Jagiellonian Univ.,Medyczna 9, 30-688 Krakow, Poland): Identification and determination of gliclazide and its impurities in variousmedicines by thin-layer chromatography and densitometry. J. Planar Chromatogr. 14, 183-187 (2001). TLC ofgliclazide and impurities on silica gel with chloroform - methanol 19:1. Detection by spraying with 0.2% ninhydrin inmethanol and heating at 105°C for 3 min. Densitometry at 226 nm. The method is characterized by high sensitivity (15ng), linearity over a wide concentration range (0.06 to 0.30 mg/ml), and high recovery (100.23%).

32a

88 123 D. KULKARNI, P.A. SUKHADIA, M. FRANCIS, R.T. SANE*, (*S.P. Mandal's Ramnarain Ruia College, Chem. Dept.,



88 123 D. KULKARNI, P.A. SUKHADIA, M. FRANCIS, R.T. SANE*, (*S.P. Mandal's Ramnarain Ruia College, Chem. Dept.,Matunga, Mumbai-400019, India): HPTLC method for determination of piperine from Piper nigrum Linn. IndianDrugs 38 (6), 323-326 (2001). HPTLC on silica gel with hexane - ethyl acetate 7:3. Quantitation by densitometry at 340nm.

32e

88 124 J.K. LALLA*, P.D. HAMRAPUKAR, H.M. MAMANIA, (*Prin. K.M.K. College of Pharmacy, Worli, Mumbai 400018, India): Triphala Churna - from raw material to finished products. Indian Drugs 38 (2), 87-94 (2001). HPTLC of gallic acidon silica gel for quality control of Triphala.

32e

88 125 D. LI (Li Dexun), (Zhaoton Inst. Drug Cont., Zhaotong, Yunnan, 657000, P.R. China): (Study of the identification ofZhuangguguanjie pills by thin-layer chromatography. ) (Chinese). J. Chinese Trad. Patent Med. (Zhongchengyao)23 (6), 452-453 (2001). TLC on silica gel with benzene - ethyl acetate 9:1. Detection under UV 365 nm. Identification byfinger print technique. Comparison of the procedures with that collected in China Pharmacopoeia.

32c

88 126 H. LI (Li Hongling)*, G. YANG (Yang Guangde), L. HE (He Longchong), (*Pharm. Coll., Shihezi Univ., Shihezi Xinjiang832002, P.R. China): (Identification and the active component determination of Xingangping. ) (Chinese).Chinese J. Pharm. Anal. (Yaowu Fenxi Zazhi) 21 (15), 348-350 (2001). TLC on silica gel with chloroform - ethyl acetate- methanol - water 15:40:22:10. Detection by spraying with 10% sulfuric acid in ethanol and heating at 105°C for 10min, and under UV 365 nm. Identification by finger print technique.

32c

88 127 L. LI (Li Lanzi)*, S. GUO (Guo Suli), L. SONG (Song Li), (*Jiaozuo Inst. Drug Cont., Jiaozu, Henan 454100, P.R. China): Identification of Cinnamomun cassia presl. J. Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32 (17), 651-652(2001). TLC on silica gel with benzene - ethyl acetate - acetic acid 18:1:1. Detection by spraying with 2% KMnO4solution. Identification by finger print technique, combined with their UV spectra.

32e

88 128 S. LI (Li Suqin), (Dept. Biochem., Inst. Exp. Med., Hebei Acad. Med. Sci., Shijianzhuang 050021 P.R. China): (Separation and purification of an endogenuous inhibitor of sodium pump from Chansu by thin-layerchromatography and reversed-phase high performance liquid chromatography. ) (Chinese). Chinese J.Chromatogr. (Sepu) 19 (6), 555-557 (2001). TLC on silica gel with chloroform - methanol - water 15:4:1. Detectionunder UV. Further purification of the TLC fraction with Rf 0.55 inhibiting the sodium pump by HPLC. Measuring thebioactivity by 86Rb-uptake into human red blood cells. Discussion of the behavior in inhibiting sodium pump.Comparison to onabain and proscillaridin A.

32

88 129 Y. LI (Li Yunxin), (Chengde Chinese Med. Group Co., Ltd., Chengde, Hebei 067000, P.R. China): (Determination ofpuerarin in Radix puerariae by TLC-scanning. ) (Chinese). J. Chinese Trad. Patent Med. (Zhongchengyao) 23 (6),444-445 (2001). TLC on silica gel with chloroform - methanol - water 60:20:1 for the first development and 100:20:1 forthe second. Detection under UV 254 nm. Quantitation by densitometry at 254 nm.

32c

88 130 B. LIAWRUANGRATH, S. LIAWRUANGRATH*, (Fac. Pharm. & Fac. Sci. Chang Mai Univ., Chan Mai 50200, Thailand): High-performance thin-layer chromatographic determination of erythromycin in pharmaceutical preparations. Chromatographia 54 (5/6), 405-408 (2001). TLC on silica gel with methanol by using AMD. Detection by spraying with10% sulfuric acid in ethanol and heating at 100°C for 10-15 min. Quantitation by densitometry at 410 nm. Discussion ofthe optimization of the conditions of the procedure, and the interference.

32c

88 131 J. LIN (Lin Jiangqin)*, T. LIANG (Liang Tong), H. ZHUANG (Zhuang Haiqi), L. MO (Mo Lier), T. WU (Wu Tie), Q. LI (LiQingnan), (*Dept. Chem., Guangdong Coll. Med., Zhanjiang 524023, P.R. China): (Study of the quality standards forZhuanggushenbao capsules. ) (Chinese). Chinese J. Modern App. Pharm. (Zhangguo Xiandai Yingyong YaoxueZazhi) 18 (3), 192-194 (2001). TLC on silica gel with 1) toluene - ethyl acetate 19:1, 2) chloroform - methanol - water13:7:2, 3) ethanol - water 11:9. Detection 1) by spraying with 10% sulfuric acid in ethanol and heating at 110°C for5 min, and under UV 365 nm, 2) by spraying with 1% AlCl3 in ethanol, heating, and under UV. Identification by fingerprint technique.

32c

88 132 M. LIN (Lin Minyan)*, CH. WANG (Wang Chao), Y. WANG (Wang Yufeng), (*Yiling Acad. Med., Hebai Provin.,



88 132 M. LIN (Lin Minyan)*, CH. WANG (Wang Chao), Y. WANG (Wang Yufeng), (*Yiling Acad. Med., Hebai Provin.,Shijiazhuang 050091, P.R. China): (An improved procedure for the separation of tanshinone IIA in Radix SalviaeMiltiorrhizae. ) (Chinese). J. Chinese Trad. Patent Med. (Zhongchengyao) 23 (7), 527-528 (2001). TLC on silica gelwith petroleum ether (60-90°C) - ethyl acetate 4:1. Detection under day light. Comparison of the result with thatobtained with benzene - ethyl acetate 10:1. Discussion of the merits of the improved procedures.

32c

88 133 S. LIN (Lin Sui)*, S. DENG (Deng Sishan), R. LIU (Liu Ren), (*Fujian Inst. Med. Sci., Fuzhou 350001, P.R. China): (Studies of quality standard of Aduola Fuzhenglin capsules. ) (Chinese). J. Chinese Trad. & Herb. Drugs(Zhongcaoyao) 32 (6), 506-507 (2001). TLC on silica gel with chloroform - methanol -water 13:7:2. Detection byspraying with 10% sulfuric acid in ethanol and heating at 110°C. Identification by comparison with the standard.Quantitation of astragaloside by densitometry at 520 nm.

32c

88 134 J. LU (Lu Jinqing)*, N.YU (Yu Nancai), K. JU (Ju Kefei), X. LIN (Lin Xinqiao), H. ZHAO (Zhao Hengfa), (*Hubai Coll.Trad. Chin. Med., Wuhan 430061, P.R. China): (Studies of the quality standards for Juqin granules. ) (Chinese). J.Chinese Trad. Patent Med. (Zhongchengyao) 23 (6), 404-407 (2001). TLC on silica gel with 1) chloroform - methanol -acetic acid 20:3:1, 2) chloroform - methanol -water 14:6:1, 3) hexane - ethyl acetate 9:1, 4) chloroform - methanol 5:1.Detection 1) by spraying with 10% sulfuric acid in ethanol and heating at 105°C, 2) by spraying with vanillin in sulfuricacid solution and heating at 105°C, 3) under UV 365 nm. Identification by finger print technique. Quantitation of baicalinby HPLC.

32c

88 135 P. LU (Lu Peng)*, D. FANG (Fang Demin), S. TANG (Tang Suping), (*Tianjin Hosp., Tianjin 300211, P.R. China): (Determination of anthraquinone in Xiaozhongsanyu ointment by K-ratio method. ) (Chinese). J. Chinese Trad. &Herb. Drugs (Zhongcaoyao) 32 (7), 608-609 (2001). TLC on silica gel with petroleum ether (30-60°C) - ethyl formate -formic acid 15:5:1. Detection under UV 365 nm and exposure to NH3 vapor. Identification by finger print technique.Quantitation by K-ratio spectrophotometry.

32c

88 136 A. LUDWICZK*, A. NAJDA, T. WOLSKI, T. BAJ, (*Dept. of Pharmacogn., Med. Univ., 20-007 Lublin, 12 Peowiakow St.,Poland): Chromatographic determination of the content and the composition of extracts and essential oils fromthe fruits of three stalk celery varieties (Apium Graveolens L. var. Dulce Mill. Pers.). Proc. Intern. Symp. on PlanarSeparations, Planar Chromatography 2001, pp. 103-111. TLC of essential oils and extracts (e.g. DL-limonene,DL-a-pinene, b-selinene, myrcene, trans-sabinyl acetate) on silica gel with n-hexane and n-hexane - ethyl acetate 3:1.Visualization under UV 254 nm followed by spraying with Godin's reagent (A: 5% solution of sulfuric acid in ethanol; B:1% vanillin solution in ethanol). Videodocumentation.

32e

88 137 B. MANISHA, K.S. LADDHA* (Pharm. Div., UDCT, Matunga, Mumbai-400019, India): Studies on Blepharis edulisseeds. Indian Drugs 38 (10), 542-542 (2001). TLC hydrolyzed extract mixture on silica gel (impregnated with boricacid and activated) with acetic acid - chloroform - water 8:5:1. Detection by spraying with aniline phthalate reagent. TLCof two selectively hydrolyzed fractions on silica gel with acetonitrile - water 17:3. Detection by spraying with sulfuric acid- water 1:3 and heating. Presence of b-galactose and fructose was confirmed.

32

88 138 X. MAO (Mao Xiangjun)*, H. XONG (Xong Huilin), Q. XU (Xu Qianli), R. LIN (Lin Ruichao), (*Guizhou Provin. Inst. DrugCont., Guiyang 550004, P.R. China): (Determination of gallic acid in Relinqing capsules and granules by thin-layerchromatography. ) (Chinese). Chinese J. Pharm. Anal. (Yaowu Fenxi Zazhi) 21 (5), 364-365 (2001). TLC on silica gelwith toluene - ethyl acetate - formic acid 150:270:31. Detection by spraying with 1% FeCl3 solution. Quantitation bydensitometry at 530 nm.

32c

88 139 G. MATYSIK*, M. WOJCIAK-KOSIOR, J. KOWALSKI, (*Dept. of Inorg. and Anal. Chem., Med. Acad., Staszica 6,20-081 Lublin, Poland): Application of multiple-gradient thin-layer chromatography for the quantitativedetermination of convallatoxine in Herba Convallariae. J. Planar Chromatogr. 14, 191-193 (2001). HPTLC ofconvallatoxine on silica gel by multiple-gradient thin-layer chromatography with ethyl acetate - methanol - acetic acid.Development 'face down' in a horizontal chamber. Detection of spots with several reagents reported in the literature;quantitation by densitometry at 366 nm.

32e

88 140 S.N. MEYYANATHAN*, G.V.S. RAMASARMA, B. SURESH, (*Dept. of Pharm. Chem., J.S.S. Coll. of Pharm.,



88 140 S.N. MEYYANATHAN*, G.V.S. RAMASARMA, B. SURESH, (*Dept. of Pharm. Chem., J.S.S. Coll. of Pharm.,Ootacamund-643 001, Tamilnadu, India; [email protected]): Analysis of finasteride in pharmaceuticalpreparations by high performance thin layer chromatography. J. Planar Chromatogr. 14, 188-190 (2001). TLC offinasteride and loratadine on silica gel plates (prewashed with methanol) with chloroform - ethyl acetate 3:2. Evaluationby densitometry at 228 nm. The linear range of the analysis was 0.2 - 2.0 µg, recovery 101.28%. New simple, precise,rapid, selective TLC method.

32a

88 141 K. MIGLECZI*, I. HAZAI, K. JEMNITZ, M. PATFALUSI, (*Inst. for Drug Res., Dept. of Pharmacokinetics, Pf. 17, H-1325Budapest, Hungary): Thin-layer chromatographic characterization of metabolites formed from a potentialantiepileptic drug GYKI-47 261. Proc Intern. Symp. on Planar Chromatography, Planar Separations 2001, pp. 33-44.TLC of 14C-labeled GYKI-47 261 (6-(4-aminophenyl)-8-chloro-2-methyl-11H-imidazol [1,2c][2,3]benzodiazepine) andmetabolites on silica gel with chloroform - n-hexane - ethanol - conc. NH3 75:15:9:1 as the best eluent of 31 mobilephases tested. Detection of radiolabeled compounds by means of contact autoradiography and under UV 366 nm.

32b

88 142 G. MISZTAL*, R. SLIBINSKI, (*Dept. of Med. Chem., Med. Acad., 6 Chodzki, 20-093 Lublin, Poland): Chromatographic analysis of new antidepressant drugs by normal- and reversed-phase TLC. J. PlanarChromatogr. 14, 300-304 (2001). TLC of 6 antidepressant substances (fluoxetine, fluvoxamine, paroxetine nefazodone,moclobemide, citalopram) on silica gel and RP-18. Optimization of retention and selectivity on RP-18 by changing thepH and the concentration of organic solvent (methanol, acetonitrile, tetrahydrofuran) in the aqueous phase;development in horizontal chambers. The best separation was achieved on silica gel with benzene - acetone - ethanol -NH3 9:7:2:1. Detection under UV 254 nm; in addition videodensitometry.

32a

88 143 K. MO (Mo Kefeng)* M. YIN (Yin Meifen), (*Nanning Municip. Inst. Drug Cont., Nanning 530001, P.R. China): (Determination of berberine hydrochloride in Fuyinjie by thin-layer chromatography. ) (Chinese). Chinese J.Hosp. Pharm. (Zhongguo Yaoxue Zazhi) 21 (11), 667-669 (2001). TLC on silica gel with butanol - acetic acid - water7:1:2. Detection and quantitation by densitometry at 430 nm.

32c

88 144 Y. NI (Ni Yan)*, G. LIN (Lin Guixia), X. LIN (Lin Xia), X. LI (Li Xianrong), (*Shanxi Inst. Chin. Trad. Pharm. & Med.,Taiynan 030012, P.R. China): (Determination of Scutellarin in Xingan pills by thin-layer chromatography. ) J.Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32 (6), 515-517 (2001). TLC on polyamide with 30% acetic acid.Detection under UV 365 nm. Quantitation by densitometry at 294 nm.

32c

88 145 Y. NIU (Niu Yujuan)*, SH. ZHANG (Shixuan), (*Dalian Municip. Inst. Drug Cont., Dalian 116021, P.R. China): (Identification of seven 4-quinolones drugs. ) (Chinese). Chinese J. Pharm. Anal. (Yaowu Fenxi Zazhi) 21 (3),204-206 (2001). TLC on silica gel with chloroform - methanol - NH3 15:10:3. Detection under UV 254 nm. Identificationby Rf value comparison, combined with UV spectra, and HPLC method.

32c

88 146 B. OSCIK-MENDYK, M. JANICKA*, L. KWIETNEWSKI, (*Fac. of Chem., Maria Curie-Sklodowska Univ., MariaCurie-Sklodowska Sq. 3, 20-031 Lublin, Poland): The separation of catecholamines by planar chromatography. Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp. 235-240. TLC of epinephrine,norepinephrine, dopamine, normethanephrine, methanephrine, 4-hydroxy-3-methoxymandelic acid and serotonin onsilica gel and silanized silica gel with buffer (pH = 6.98) - methanol 1:1. Visualization by exposure to iodine vapor;detection by densitometry at 200 nm. New and simple TLC procedure.

32f

88 147 L. PAN (Pan Linmei)*, Y. WANG (Wang Yongshan), (*Plant Drug Cont., Nanjing Univ. TCM, Nanjing 210029, P.R.China): (Analysis of Radix et Rhizoma Rhei and Rhizoma polygoni cuspidati in Bingganning granules. )(Chinese). J. Chinese Trad. Patent Med. (Zhongchengyao) 23 (8), 562-564 (2001). TLC on silica gel with the upperphase of petroleum ether (30-60°C) - ethyl formate - formic acid 15:5:1. Detection under UV 365 nm. Identification byfinger print technique.

32c

88 148 S. PERNECZKI, A. KERNER, E. HIVEGI*, A. BENKÖ, (*Nat. Inst. of Forensic Toxicology, 1146 Budapest, Varannó u.



88 148 S. PERNECZKI, A. KERNER, E. HIVEGI*, A. BENKÖ, (*Nat. Inst. of Forensic Toxicology, 1146 Budapest, Varannó u.2-4, Hungary): New OPLC screening for drugs acting on the central nervous system in post-mortem tissues. Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp. 223-233. OPLC of 47 drugs(benzodiazepines and 'non-benzodiazepines': new type psychotics) on HPTLC silica gel with ethyl acetate - chloroform3:7 as best mobile phase for benzodiazepines. Densitometric evaluation by densitometry at the absorption maxima ofthe respective compounds.

32d

88 149 H. PIAO (Piao Huaying)*, J. LIN (Lin Jiping), L. SUN (Sun Long), (*Inst. TCM, Xiamen Guilong Group Co. Ltd., Xiamen361006, P.R. China): (Study of quality standards for Lingyuangqingfei capsules. ) (Chinese). J. Chinese Trad.Patent Med. (Zhongchengyao) 23 (8), 566-569 (2001). TLC on silica gel with 1) chloroform - methanol 3:1, 2)cyclohexane - ethyl acetate 3:1. Detection 1) by spraying with 10% sulfuric acid in ethanol and heating at 110°C for 5min, 2) by spraying with 5% FeCl3 in ethanol and heating. Identification by finger print technique.

32c

88 150 A.D. PILLAI, M.M., RAVISHANKARA, R.D. HANDRAL*, (*Basaveshwar College of Pharmacy, Bagalkot-587101,Karnataka, India): HPTLC method for estimation of lovastatin from tablets. Indian J. Pharm. Sci. 63 (5), 420-422,(2001). HPTLC on silica gel with toluene - methanol 3:1. Quantitation by densitometry at 239 nm. The method issuitable for routine quality control with high sample throughput.

32

88 151 T.V. POLENOVA, A.G. BORZENKO, I.V. MARUTSENKO, L.A. REVELSKY, (Dept. Anal. Chem., Moscow State Univ.,Russia): Screening of psychotropic drugs in human hair based on high-performance thin-layer chromatographyand microliquid extraction. J. Chromatogr. Sci. 39 (7), 293-296 (2001). Presentation of a procedure combining TLCwith microliquid extraction as a psychotropic drug testing method for hair, involving some widely distributedantidepressants, neuroleptics, and sedative drugs. Optimization of the experimental conditions for all of the consequentsteps for the screening detection of the model samples. Use of the procedure for a fast and cost-effective analysis ofabuse.

32c, f

88 152 J. POTHIER, N. GALAND, C. VIEL, (Lab. of Pharmacogn., Fac. of Pharm., 31 avenue Monge, 37200 Tours, France): Separation of opiates and derivatives - analgesics, antitussives and narcotic compounds - by differenttechniques of planar chromatography (TLC, OPLC, AMD). Proc. Intern. Symp. on Planar Separations, PlanarChromatography 2001, pp. 321-326. TLC/AMD and OPLC (Optimum Performance Laminar Chromatography) of Opiumextract and morphine , codeine, thebaine, papaverine, noscapine, ethylmorphine, pholcodine, heroin, buprenorphine,dextromethorphan, methadone as reference substances: TLC of Opium alkaloids on silica gel with toluene - acetone -ethanol - 28% NH3 45:45:7:1. OPLC on aluminium oxide with ethyl acetate as mobile phase and AMD with methanol,acetone, ethyl acetate, ethyl acetate/dichloromethane, dichloromethane. TLC is quick, simple, cheap, detection is easyand permits densitometry; OPLC is a fast method; AMD presents the best resolution, no diffusion of spots, elutionwithout oxidation and is considered as a very interesting method for densitometry.

32d, e

88 153 R.T. SANE*, FRANCIS MARY, KHEDKAR SACHIN, PAWAN SAGAR, MOGHE ATUL, (*Therapeutic Drug MonitoringLab., 194. Scheme No. 6, Road No. 15, Sion, Koliwada, Mumbai-400022, India): Simultaneous HPTLC determinationof pseudoephedrine sulfate and loratadine from their combined dosage form. Indian Drugs 38 (8), 436-438(2001). HPTLC on silica gel with hexane - dichloromethane - triethylamine 9:8:1. Densitometry at 235 nm with diltiazemas internal standard.

32

88 154 G. SHENG (Sheng Guorong)*, Y. XIE (Xie Yong), (*Dermatol. Station of Nantong, Nantong, Jiangsu 226006, P.R.China): (The experimental study of the quality criteria of antileukoderm tincture. ) (Chinese). Chinese J. ModernApp. Pharm. (Zhangguo Xiandai Yingyong Yaoxue Zazhi) 18 (3), 223-225 (2001). TLC on silica gel with 1) petroleumether (60-90°C) - ethyl acetate 2:1, 2) ethyl acetate - acetone - formic acid - water 5:5:1:1. Detection 1) under UV 365nm, 2) by spraying with 10% sulfuric acid in ethanol and heating at 110°C for 10 min. Identification by finger printtechnique. Quantitation of hydrocortisone acetate by derivative spectrophotometry.

32c

88 155 C. SHI (Shi Cunyuan), (Zhejiang Jianfeng Pharm. Co. Ltd., Jinhua, Zhejiang 321000, P.R. China): (Study of thequality standard of Yufeng Ningxindi pills. ) (Chinese). J. Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32 (10),894-895 (2001). TLC on silica gel with chloroform - methanol - water 28:10:1. Detection under UV 365 nm. Identificationby finger print technique. Quantitation of puerarin by HPLC.

32c



88 156 H.D. SMOLARZ*, G. MATYSIK, (*Dept. of Pharm. Botany, Med. Univ., 4 Staszica Str., 20-081 Lublin, Poland): Determination of persilben in some species of Polygonum L. J. Planar Chromatogr. 14, 199-202 (2001). HPTLCof persilben (3,5-methoxy-2-hydroxy-E-stilbene) on silica gel and diol-modified silica gel with mixtures of hexane,chloroform and methanol for a two-step gradient program. Quantitation by densitometry at 360 nm.

32e

88 157 G. SONG (Song Guanying)*, G. WU (Wu Guangeni), Y. LI (Li Yuqin), (*Quiten Inst. Drug Cont., Quiten, Xinjiang833200, P.R. China): (Identification of Marinda officinalis How and Akebia trifoliata (Thunb. ) Koidz.) (Chinese). J.Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32 (7), 650-651 (2001). TLC on silica gel with ethanol - water 3:2.Detection under UV. Identification by finger print technique combined with UV spectra comparison.

32e

88 158 Y. SONG (Song Yuliang)*, J. CHEN (Chen Jianzhen), G. LU (Lu Guiyuan), (*Zheijiang Coll. TCM, Hangzhou 310053,P.R. China): (Study of the quality standard of Jinwang capsules. ) (Chinese). J. Chinese Trad. Patent Med.(Zhongchengyao) 23 (9), 645-647 (2001). TLC on silica gel with petroleum ether (60-90°C) - ethyl acetate - acetic acid30:10:1. Detection under UV 254 nm. Identification by finger print technique. Quantitation of 10-hydroxy-2-decylenic acidby spectrophotometry at 210 nm and 235 nm.

32c

88 159 Q. SU (Su Qiang)*, Y. NI (Ni Yan), X. HAO (Hao Xuliang), X. LI (Li Xianrong), (*Shanxi Provin. Inst. TCM, Taiyuan030012, P.R. China): (Study of the quality standards for Qingchunle capsules. ) (Chinese). J. Chinese Trad. PatentMed. (Zhongchengyao) 23 (7), 486-488 (2001). TLC on silica gel with 1) chloroform - acetone - formic acid 25:10:4, 2)methanol - acetone - chloroform - NH3 15:20:50:3, 3) chloroform - ethyl acetate - methanol - water 15:40:22:10.Detection 1) by spraying with 1% FeCl3 in ethanol , 2) by spraying with 2% FeCl3 in 50% ethanol - 2% potassiumferricyanide 1:1. 3) by spraying with 10% sulfuric acid in ethanol and heating at 105°C and under UV 365 nm.Identification by finger print technique. Quantitation of paeoniflorin by densitometry at 230 nm.

32c

88 160 N. RANI SULOCHANA, K. BALAJI, VEERESHAM CIDDI*, (*Fac. of Pharm. Sci., Kakatiya Univ., Warangal-506007,India): Production of hypericin from tissue culture of hypericum perforatum. Indian J. Pharm. Sci. 63 (5),431-433 (2001). TLC on silica gel with ethyl acetate - formic acid - acetic acid - water 100:11:11:27. Detection byspraying with 10% pyridine in ethanol. Comparison with PR-HPLC.

32e

88 161 A.C. SUTHAR, M.M. BANAVALIKER, M.K. BIYANI, K. INDRA PRIYADARSHANI, V., SUDARSAN, H. MOHAN, (AjantaPharma Ltd., Res. Centre, 98-Govt. Industrial Estate, Kandivali (W), Mumbai-400103, India): A HPTLC method forquantitative estimation of charantin in Momordica charantia Lin. Indian Drugs 38 (5), 230-235 (2001). HPTLC onsilica gel with benzene - methanol 4:1. Visualization by spraying with Libermann-Burchard reagent. Quantitation bydensitometry at 536 nm.

32e

88 162 C. VIEL, N. GALAND, J. DOLLET, J. POTHIER, (Lab. of Pharmacogn., Fac. of Pharm., 31 avenue Monge, 37200Tours, France): Analysis by planar chromatography (TLC and AMD) of cannabinoids. Proc. Intern. Symp. onPlanar Separations, Planar Chromatography 2001, pp. 309-312. HPTLC of hemp extracts (cannabidiol, cannabinol) andD8THC and D9THC (as standards) on silica gel with ether - hexane 1:4 for normal HPTLC and two gradients(dichloromethane, dichloromethane/hexane 50/50, hexane; 25 steps or diethyl ether, diethyl ether/hexane 50/50,hexane in 20 steps) as mobile phases. Visualization by spraying with Fast blue B reagent (0.5 p. 100 in methanol). TheAMD technique gives more information about the composition of different samples of Indian Hemp and openspossibilities to determine the geographic and operation mode of preparation of different samples.

32d

88 163 H. WANG (Wang Hongzhi)*, J. LIN (Lin Junhong), L. LI (Li Lihua), J. WEI (Wei Junfeng), (*Nankai Hosp., Tianjin300100, P.R. China): (Determination of astragaloside in Yigyangxue infusion by thin-layer chromatography. )(Chinese). J. Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32 (8), 703-704 (2001). TLC on silica gel with ethyl acetate -butanone - formic acid - water 6:1:1:1. Detection by spraying with 10% sulfuric acid and heating at 102°C for 5 min.Quantitation by densitometry at 530 nm.

32c

88 164 H. WANG (Wang Hong)*, X. WANG (Wang Xuewei) SH. CHEN (Chen Shizhong), N. LI (Li Nian), (*Dept. Nat. Med.,



88 164 H. WANG (Wang Hong)*, X. WANG (Wang Xuewei) SH. CHEN (Chen Shizhong), N. LI (Li Nian), (*Dept. Nat. Med.,Sch. Pharm. Sci., Med. Coll., Beijing Univ., Beijing 100083, P.R. China): (Physiochemical identification of threeplants of Echinacea Moench. ) (Chinese). J. Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32 (10), 934-936 (2001).TLC on silica gel with 1) toluene - ethyl acetate 13:10, 2) butanol - acetic acid - water 5:5:1. Detection 1) by sprayingwith vanillin-sulfuric acid reagent and heating at 105°C, 2) under UV 365 nm. Identification by finger print technique,combined with UV absorption spectrometry.

32f

88 165 X. WANG (Wang Xinchun)*, Q. LI (Li Qin), Z. Han (Han Ziyan),(*1st Hosp., Kaifeng Med. Col., Kaifeng 475001, P.R.China): (Identifcation of Zaocys dhumnades and its adulterant Dinodon rufozonatum. ) (Chinese). Chinese J.Modern App. Pharm. (Zhangguo Xiandai Yingyong Yaoxue Zazhi) 18 (4), 284-287 (2001). TLC on silica gel with 1)chloroform - acetone 19:1, 2) butanol - acetic acid - water 4:4:1. Detection 1) by spraying with 10% phosphomolybdicacid and heating at 110°C for 10 min, 2) by spraying with 10% sulfuric acid and heating at 110°C for 10 min and underUV 365 nm, 3) under UV 365 nm, 4) by spraying with 0.2% ninhydrin in ethanol and heating at 105°C for 10 min.Identification by finger print technique.

32b

88 166 X. WANG (Wang Xianyou)*, J. QU (Qu Jianying), Y. JING (Jing Yongsheng), Y. ZHANG (Zhang Yun), Dept. Med.,Henan Univ., Kaifeng 475001,P.R. China): (Determination of ciprofloxacin in Bingqing by thin-layerchromatography and UV spectrophotometry. ) (Chinese). Chinese J. Chem. Res. (Huaxue Yanjiu) 12 (2), 46-48(2001). TLC on silica gel with chloroform -methanol - ethanol - NH3 15:8:8:7. Detection under UV 365 nm. Quantitationby UV spectrophotometry at 273 nm after elution from the spot.

32c

88 167 Y. WANG (Wang Yumei)*, H. ZHAO (Zhao Huimin), B. SONG (Song Baopeng), A. ZHENG (Zheng Anqin), (*Jixi Munic.Inst. Drug Cont., Jixi, Helongjiang, 158100, P.R. China): (Identification of proralen and Szechwan lovge rhizome inBushenyinao tablets by thin-layer chromatography. ) (Chinese). J. Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32(7), 614-615 (2001). TLC on silica gel with hexane - ethyl acetate 4:1. Detection under UV 365 nm. Identification byfinger print technique.

32c

88 168 ZH. WANG (Wang Zhenhua)*, Q. DU (Du Qin), H. LIN (Lin Hao), X. XU (Xu Xiaofeng), J. ZHANG (Zhang Junrong),(*Coll. TCM, Guangzhou Univ. TCM, Guangzhou 510407, P.R. China): (Culturing of hairy root of Polygonummultiforum and determination of its chrysophanol content. ) (Chinese). J. Chinese Trad. & Herb. Drugs(Zhongcaoyao) 32 (8), 695-696 (2001). TLC on silica gel with petroleum ether - ethyl acetate - acetic acid 20:4:3.Detection under daylight. Quantitation by densitometry at 435 nm. Discussion of the distribution of chrysophanol in thecultured root and the main part of the drug.

32e

88 169 L. WEI (Wei Lixin)*, B. ZHANG (Zhang Baochen), Y. TAO (Tao Yanduo), Y. DU (Du Yuzhi), (*Biol. Inst. NorthwestPlateau, Chin. Acad. Sci., Xining 810008, P.R. China): (Studies on quality control of Xuelong capsules. ) (Chinese).J. Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32 (6), 510-512 (2001). TLC on silica gel with cyclohexane - ethylacetate 1:1. Detection by spraying with potassium iodobismuthate reagent. Identification by comparison with thestandard. Quantitation of L-oxoproline by spectrophotometry at 561 nm.

32c

88 170 L. WILLIAMS*, O. BERGERSEN, (*SINTEF Appl. Chem., P.O. Box 124 Blindern, N-0314 Oslo, Norway): Towards anintegrated platform for combinatorial library synthesis and screening. Proc. Intern. Symp. on Planar Separations,Planar Chromatography 2001, pp. 81-90. An amine in dichloromethane was applied as a single spot to a silica gelplate. A solution of a sulfonyl chloride was then applied to the same area. The solvent was allowed to evaporate fromthe plate which was then irradiated in a microwave oven. TLC with methanol - dichloromethane 1:9. Visualization underUV 254 nm or by spraying with ninhydrin. Product spots could be analyzed by MS resp. by NMR and GCMS afterelution (typical reference standards used were sulfaguanidine, sulfapyridine, sulfathiazole, sulfadiazine,sulfamethoxazole and amphotericin). Screening was done with a bacteria suspension of Serracia marcescens orBacillus subtilis in an agarose containing medium on the TLC plate.

32a

88 171 W. WU (Wu Wenying)*, X. LI (Li Xinghua), J. LEI (Lei Jianlin), J. WANG (Wang Jinqin), ZH. DUAN (Duan Zhongming),(*2nd Hosp., Xian Jiaotung Univ., Xian 210004, P.R. China): (Determination of retinoic acid in compound retinoicacid capsules by thin-layer chromatography. ) (Chinese). Chinese J. Hosp. Pharm. (Zhongguo Yaoxue Zazhi) 21(1), 411-413 (2001). TLC on silica gel with chloroform - methanol - formic acid 30:4:1. Detection under UV 254 nm.Quantitation by densitometry at 290 nm.

32c



88 172 W. WU (Wu Weiping)*, ZH. XIAO (Xiao Zhongping), (*Zjenjiang Factory TCM, Zhenjiang Jiangsu 212005, P.R. China): (Study of the quality standard for Weian capsules. ) (Chinese). J. Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32(6), 518-519 (2001). TLC on silica gel with 1) cyclohexane - ethyl acetate - acetic acid 17:2:1, 2) chloroform - methanol -ethyl acetate 8:4:1, 3) butanol - acetic acid - water 4:1:1. Detection 1) under UV, 2) by spraying with 10% sulfuric acid inethanol and heating, 3) by spraying with 5% vanillin-sulfuric acid reagent and heating, 4) by spraying with 2% ninhydrinin ethanol and heating. Identification by finger print technique.

32c

88 173 X. WU (Wu Xuefen)*, Y. BAI (Bai Yan), (*Henan Coll. TCM, Zhengzhou 450008, P.R. China): (Extractions of activecomponents from Flos Lonicerae and their bacteriostatic test. ) (Chinese). J. Chinese Trad. Patent Med.(Zhongchengyao) 23 (6), 448-449 (2001). TLC on silica gel with butanol - acetic acid - water 4:1:2. Detection under UV.Identification of chlorogenic acid by standard comparison. Use of the procedures for monitoring the extraction process.

32c

88 174 Y. YAN (Yan Yaodong)*, Y. JIN (Jin Ying), ZH. ZHOU (Zhou Zhigang), L. ZHANG (Zhang Lei), (*No. 466 HOSP. PLA,Beijing 100089, P.R. China): (Determination of oleanolic acid of Xuefuzhuyu Pills by thin-layer chromatography. )(Chinese). Chinese J. Hosp. Pharm. (Zhongguo Yaoxue Zazhi) 21 (12), 719-721 (2001). TLC on silica gel withcyclohexane - acetone - ethyl acetate 4:2:1. Detection by spraying with 10% sulfuric acid in ethanol and heating at 90°Cfor 10 min. Quantitation by densitometry at 535 nm.

32c

88 175 G. ZHANG (Zhang Geng)*, Q. LU (Lu Quande), SH. GONG (Gong Shixong), (*1st Hosp. Wuhan, Wuhan 430022, P.R.China): (The quality monitoring of Fuke lotion by thin-layer chromatography. ) (Chinese) Chinese J. Hosp.Pharm. (Zhongguo Yaoxue Zazhi) 21 (9), 433-434 (2001). TLC on silica gel with chloroform - ethyl acetate - methanol -NH3 80:10:10:1. Detection by spraying with potassium iodobismuthate solution. Identification by finger print technique.

32c

88 176 H. ZHANG (Zhang Hongjun)*, Y. FU (Fu Yanmin), B. LI (Li Baijun), Y. Tian (Tian Yao), (*Siping Municip. Inst. DrugCont., Siping, Jilin 136000, P.R. China): (An improved procedure for the identification of Shugan pills bythin-layer chromatography. ) (Chinese). J. Chinese Trad. Patent Med. (Zhongchengyao) 23 (10), 777-778 (2001).TLC on silica gel with hexane - chloroform - methanol 15:8:2. Detection by exposure to iodine vapor, and under UV 365nm. Identification by finger print technique.

32c

88 177 J. ZHANG (Zhang Juanjuan), (Xiamen Fac. TCM, Xiamen, Fujian 361009, P.R. China): (Determination of grapholideand andrographolide in Qinggan Chuanxinlian tablets by thin-layer chromatography. ) (Chinese). J. ChineseTrad. & Herb. Drugs (Zhongcaoyao) 32 (9), 806-807) (2001). TLC on silica gel with chloroform - acetone 2:1. Detectionunder UV 254 nm. Quantitation by densitometry at 230 nm for grapholide and at 263 nm for andrographolide.Discussion of use of the procedure in the quality control of the medicine.

32c

88 178 Z. ZHANG (Zhang Zunting)*, Q. LIN (Lin Qianguang), (*Chem. Dept. Shanxi Norm. Univ., Xian 710062, China): (Determination of emodin in Dahuang Zhechong pilulae by thin-layer chromatography. ) (Chinese). Chinese J.Pharm. Anal. (Yaowu Fenxi Zazhi) 21 (2), 134-136 (2001). TLC on silica gel with toluene - ethyl acetate - formic acid15:2:1. Detection under UV 365 nm. Quantitation by densitometry at 440 nm.

32c

88 179 L. ZHAO (Zho Lin), (Wenzhou Inst. Drug Cont., Wenzhou 325000, P.R. China): (An improved procedure foridentification of Huaijiao pills by thin-layer chromatography. ) (Chinese). J. Chin. Trad. Patent Med.(Zhongchengyao) 23 (11), 831-832 (2001). TLC 1) on silica gel with petroleum ether (60-90°C) - ethyl acetate 4:1, 2)on polyamide with ethyl acetate - butanone - formic acid - water 6:4:1:1. Detection 1) under UV 365 nm, 2) by sprayingwith 1% AlCl3 in ethanol and under UV 365 nm. Identification by finger print technique.

32c

88 180 R. ZHAO (Zhao Ruizhi)*, X. Qin (Quin Xiaolhui), M. LIN (Lin Maocai), (*The Cent. Lab., Guangdong Provin. TCMHosp., Guangzhou 510120, P.R. China): (Study of the methodology for the identification of Naomai II. ) (Chinese).Chinese J. Modern App. Pharm. (Zhangguo Xiandai Yingyong Yaoxue Zazhi) 18 (3), 227-229 (2001). TLC on silica gelwith 1) chloroform - ether - acetic acid 2:2:1, 2) petroleum ether (60-90°C) - ethyl acetate - acetic acid 87:6.4:3.5, 3)butanol - sulfuric acid - ethyl acetate 8:3:1, 4) ethyl acetate - formic acid - water 20:2:1. Detection 1) by spraying with10% sulfuric acid in ethanol and heating at 100°C for 7 min, 2) under UV, 3) by exposure to iodine vapor, 4) byspraying with 5% potassium iodobismuthate reagent. Identification by finger print technique. Quantitation of cholic acidby densitometry at 528 nm.

32c



32c

88 181 W. ZHONG (Zhong Weihua)*, Y. ZHAO (Zhao Yan), (*Aff. Children's Hosp., Capital Children's Inst., Beijing 100020,P.R. China): (Identification of vitamin D in Complex Calcium III pills by thin-layer chromatography. ) (Chinese).Chinese J. Hosp. Pharm. (Zhongguo Yaoxue Zazhi) 21 (3), 187-188 (2001). TLC on silica gel with cyclohexane - ether -benzene 8:4:1. Detection by spraying with sulfuric acid - aceto anhydride - 10% ethanol 1:1:8 and heating at 110°C for 5min. Identification by comparison with the standard.

32c

88 182 SH. ZHU (Zhu Shanyin), (Jiaxing Inst. Drug Cont., Jiaxing, Zheijiang 314001, P.R. China): (Identification of Cornusofficinalis from adulterant of Vitis vinifera. ) (Chinese). J. Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32 (8),745-746 (2001). TLC on silica gel with butanol - chloroform - ethyl acetate 20:5:8. Detection by spraying with acetoanhydride - sulfuric acid 9:1 and heating at 110°C at 5 min. Identification by finger print technique, combined with UVspectra comparison, and microscopic characteristic.

32e

33. Inorganic substances

88 183 A. MOHAMMAD, V, AGRAWAL, (Anal. Lab., Dept. of Applied Chem., Fac. of Eng. and Tech., Aligarh Muslim Univ.,Aligarh 202002, India): Thin-layer chromatography of anions - separation of coexisting hexacyanoferrate(II),hexacyanoferrate(III) and thiocyanate with a new mobile phase. J. Planar Chromatogr. 14, 371-377 (2001). HPTLCof 18 anions on silica gel plates with 19 different solvents comprising distilled water, pure organic solvents and mixturesof these. Detection by spraying with a saturated solution of silver nitrate in methanol, 0.2-0.5% diphenylamine in 4 mLsulfuric acid, 10% ferric chloride in 2.0 m hydrochloric acid or 0.5% ethanolic pyrogallol solution resp.

33b

35. Other technical products and complex mixtures

88 184 S.V. MAKAROVA*, B.V. TYAGLOV, (*Chem. Dept. of Moscow State Univ., Leninsky Gory, 119899, Moscow, Russia): Quantitative determination of adenosine in fermentation broths. Proc. Intern. Symp. on Planar Separations,Planar Chromatography 2001, pp. 283-292. HPTLC of adenosine, cytosine, thymidine, 2'-deoxyuridine, 2'-deoxycytidine,thymine, inosine and uridine on silica gel - after prewashing with methanol - chloroform 1:1 with 1-butanol - 2-propanol -25% NH3 - water 13:1:2:1, ethyl acetate - methanol - 25% NH3 5:2:1, and chloroform - methanol - water 830:15:3.Detection under UV at 254 nm. Quantitation by densitometry in reflectance mode at 260 nm.

35b

88 185 H. DONG (Dong Huiru)*, J. ZHANG (Zhang Jingchang), J. ZHANG (Zhang Jianjun), H. JIN (Jin Hua), (*Dept. Appl.Chem., Beijing Univ. Chem. Eng., Beijing 100029, P.R. China): (Separation of sulfonated products by thin-layerchromatography. ) (Chinese). Chinese Anal. Chem. (Fenxi Huaxue) 29 (9), 1109-1110 (2001). TLC on silica gel with1) isopropanol - ethanol - NH3 2:2:1, 2) propanol - ethanol - NH3 2:2:1, 3) isopropanol - ethanol -water 1:1:1, and 4)propanol ethanol - water 1:1:1. Detection under UV 254 nm. Identification by finger print technique.

35d

88 186 F. GHOLAMIAN*, M. CHALOOSI, S.W. HUSAIN, H. DEGHANI, (*Malek-Ashtar Univ. of Techn., Tehran, Iran): Determination of RDX and Keto-RDX in high-explosive mixtures by high-performance thin-layerchromatography. J. Planar Chromatogr. 14, 296-299 (2001). HPTLC of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX)and 2-oxo-1,3,5-trinitro-1,3,5-triazacyclohexane (Keto-RDX or K-6) on silica gel developed successively with1,2-dichloroethane - methanol 2:1 and chloroform - acetone - methanol 4:1: 1. Detection under UV 254 nm. Quantitationby densitometry at 245 nm.

35c

88 187 A. GOLC WONDRA*, B. LAPORNIK, M. PROSEK, (*Nat. Inst. of Chem., Lab. of Food Chem., Hajdrihova 19, Ljubljana,Slovenia): Evaluation the effect of plant extracts on the oxidative stability of edible oils by TLC. Proc. Intern.Symp. on Planar Separations, Planar Chromatography 2001, pp. 277-281. TLC of oil samples used for deep-frying offoods (chicken filets) on silica gel with petroleum ether - ether - acetone 90:10:1. Visualization by spraying with a 10%solution of phosphorus-molybdic acid in ethanol as well as by drying at 100°C. Quantification by videodensitometry.Quick and practical TLC method.

35b

88 188 C. JIANU*, E. ALEXA, C. CIOBANU, I. JIANU, (*Banat's Univ. of Agr. Sci. Timisoara, Calea Aradului, nr. 119, 1900



88 188 C. JIANU*, E. ALEXA, C. CIOBANU, I. JIANU, (*Banat's Univ. of Agr. Sci. Timisoara, Calea Aradului, nr. 119, 1900Timisoara, Romania): Coupling of TLC and FTIR in the analysis of cyanoethylation products of polyethoxylated (n= 3-20) superior alcohols (C12-C18). Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp.241-250. Preparative TLC of b-alkyl-polyethyleneoxy-propionitrile derivatives on silica gel with e.g. perchloroethyleneand acetone. Visualization with 2% iodine reagent. IR analysis after removal of the compound-containing sorbent andextraction as KBr tablet or solution.

35a

88 189 ST. KREIBIK*, V. COMAN, C. MARUTOIU, GH. MIHAILESCU, (*Nat. Inst. of Res. and Development for Isotopic andMolecular Technologies, P.O. Box 700, Cluj-Napoca 3400, Romania): Planar dielectrochromatography onnon-wetted thin layers. Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp. 251-263.Observation of evident electrokinetic effects (dielectroosmotic flow) on silica gel layers developed with nonpolar andpolar solvents and, as a result, improvement of the separation resolution of nonionic compounds. TLC plates with silicagel R and methanol, ethanol, n-propanol, n-butanol, pentanol, methyl ethyl ketone, benzene and mixtures thereof wereused for the experiments.

35a

88 190 P. ZHAO (Zhao Pengchen)*, Y. WANG (Wang Yanji), L. CUI (Cui Lan), X. SHI (Shi Xiaofan), Y. WANG (Wang Yanhua),(*Dept. Foren. Med., China Crim. Pol. Coll., Shenyang 110035, P.R. China): (Analysis of wring age of ballpoint penink by thin-layer chromatography. ) (Chinese). Chinese J. Chromatogr. (Sepu) 19 (4), 382-384 (2001). TLC on silicagel with isopropanol. Detection by densitometry at 580 nm. Investigation of the relationship between ratio of peakheights and writing age. Discussion of the way to enhance the reliability of the method.

35

36. Thin-layer electrophoresis

88 191 E.A. PASTORELLO*, L. FARIOLI, V. PRAVETTONI, M.G. GIUFFVIDA, C. ORTOLANI, D. FORTUNATO, CH.TRAMBAIOLI, E. SCIBOLA, A.M. CLAMARI, A.M. ROBINO, A. CONTI, (*Allergy Cent., 3rd Div. Gen. Med., OspedaleMaggiore IRCCS, Milan, Italy): Characterization of the major allergen of plum as a lipid transfer protein. J.Chromatogr. B 756 (1/2), 95-103 (2001). Identification and molecular characterization of the major allergen of plum bySDS-PAGE and immunoblotting with the sera of 23 patterns. Purification of the identified major allergen by HPLC.Further characterization by periodic-Schiff stain, isoelectro-focusing and N-terminal amino acid sequencing. Showingthat the major allergen of plum is a 9 kD lipid transfer protein, mot glycosylated, with a base character and highliyhomologous to the major allergen of peach.

36, 19

88 192 A. PETERSEN, C. MUNDT* (*Babraham Inst., Cambridge CB2 4AT, UK): Investigation on the carbohydratemoieties of glycoprotein allergens. J. Chromatogr. 13, 756 (1/2), 141-150 (2001). Presentation of an overview ofmethods combining basic procedures in glycochemistry with various applications of electrophoresis that allowinvestigating single allergens in crude extracts. Analysis of various allergen extracts, e.g. from tomato, grass pollen andbacteria.

36, 19

88 193 K. POCK*, A. BUCHACHER, A. RIZZI, D. JOSIC (*Octapharma Pharm. Produk. mbH Oberlaar Str. 235, 1100 Vienna,Austria): Characterization of clotting factor IX in plasma-derived preparations by electrophoretic techniques. J.Chromatogr. A, 921 (1), 57-67 (2001). Characterization of clotting factor IX preparations from human plasma (pdFIX)using electrophoretic methods like SDS-PAGE, isoelectric focusing and 2-D PAGE. Separation of factor IX prior to andafter activation with factor XIa by 1-D and 2-D PAGE and on isoelectric focusing gels. Identification of vitronectin byimmunological techniques as major accompanying plasma protein, separated from Factor IX and characterized byisoelectric focusing and 2D-PAGE.

36, 19

88 194 F. SCHOCKER*, W.M. BECKER, (*Forschungszentrum Borstel, Biochem. & Mol. Allergology, Parkallee 22, DE-23845Borstel, Germany): Optimization of electrophoresis for the identification of low molecular mass allergens inhazelnuts. J. Chromatogr. B 756 (1/2), 105-111 (2001). Presentation of three improved SDS-PAGE techniques usedto identify food allergens, which provided an extended resolving power for small proteins, and were applied to separatehazelnut proteins into distinct bands below 10 kDa.

36, 19

37. Environmental analysis

88 195 C. WEINS, A. SCHMITT, R. KIRN, (Landesamt f¸r Umweltschutz des Saarlandes, Don - Bosco, DE-66119 Saarbr¸cken,



88 195 C. WEINS, A. SCHMITT, R. KIRN, (Landesamt f¸r Umweltschutz des Saarlandes, Don - Bosco, DE-66119 Saarbr¸cken,Germany): Risk analysis - risk assessment - Aspects of a rational environmental and food management -Detection of bioeffective environmental compounds by bioactivity-based analysis in HPTLC. Proc. Intern. Symp.on Planar Separations, Planar Chromatography 2001, pp. 55-66. TLC of fungicides (imazalil, fenpropidin, benalaxyl,isoproteron, metoxuron, carbendazim, fenpropimorph, iprodion, epoxiconazol) on silica gel with AMD with a 33 stepsolvent gradient based on dichloromethane with acetonitrile as polar and n-hexane as nonpolar component described inDIN 38 407 Part 11. Detection of the fungicide effect of the samples using mould spores as detection agent.

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38. Chiral separations

88 196 W.J. KOWALSKI, J. NOWAK, (Inst. of Chem., Univ. of Silesia, ul. Szkolna 9, Pl - 40-006 Katowice, Poland): Molecularmodelling of planar chromatographic enantioseparation of amino acids on sorbents containing coppercomplexes of l-hydroxyproline derivatives. Proc. Intern. Symp. on Planar Separations, Planar Chromatography2001, pp. 45-54. TLC of 9 amino acids (Ala, Phe, Val, Leu, Ile, Trp, Tyr, Asp, Gln) on RP-18 impregnated with a prolinederivative and copper(II) ions with methanol - water - acetonitrile 1:1:4.

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88 197 R. SUEDEE*, T. SRICHANA, J. SAELIM, T. THAVONPIBULBUT, (*Dept. of Pharm. Chem., Fac. of Pharm. Sci., Princeof Songkla Univ., Songhkla 90110, Thailand): Thin-layer chromatographic separation of chiral drugs onmolecularly imprinted chiral stationary phases. J. Planar Chromatogr. 14, 194-198 (2001). TLC of two classes ofchiral drugs, including b-blockers and non-steroidal anti-inflammatory drugs on molecularly imprinted layers using theenantiomers of either the b-blockers, R-(+)-propanolol, R-(+)- or S-(-)-atenolol, or the NSAID, S-(+)-naproxen andS-(+)-ibuprofen, as print molecules. Acetonitrile resp. tetrahydrofuran - heptane 1:1 was used as mobile phase.Detection by spraying with a solution of anisaldehyde and heating at 120°C.

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