bpa presentation
TRANSCRIPT
What is Bisphenol A? Bisphenol A has been in use for about fifty years in the
industrialized world.
Industrial chemical used in the production of epoxy
resins and polycarbonate plastics.
Frequently used in food and beverage containers
Inner liners of metallic food and beverage containers to
prevent corrosion
Used on thermal papers like cash register and ATM
receipts
Hazard Assessment: MSDS
(Sigma Aldrich)4, 4’ – Isopropylidenediphenol (C15H16O2)
Health risk: 3* (*additional chronic hazards present)
Exposure controls:
Engineering: mechanical exhaust required
PPE: Respirator, chemical gloves, safety googles, other protective clothing
Toxicological Information: “To the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly investigated.
Environmental Information: Indication of bioaccumulation
Regulatory/Legal Superfunds Amendments and Reauthorization
Act, Section 313- BPA manufacturers must submit an
annual toxic chemical release report
Listed as an irritant in the EU and US according to
MSDS
BPA Exposures Primary exposure is via ingestion
BPA migrates from food/beverage containers
Migration is increased when container is heated
Other possible routes are inhalation and dermal
migration
Exposure is widespread; more than 90% of Americans
have been exposed to bisphenol a at some point.
Hazard Assessment: Estimated BPA
exposures (National Toxicology Program)
Population BPA micrograms/kg bw/day
Infant 0-6months formula fed 1-11
Infant 0-6months breast fed 0.2-1
Infant 6-12 months 1.65-13
Child 1.5-6 years 0.043-14.7
Adult- General Population 0.008
Adult- Occupational 0.043-100
Regulatory/Legal: European
Union The EU currently has suggested a temporary tolerable
daily intake of 5μg/kg bw/day pending further study
results, a drop from the previous TDI of 50μg/kg
bw/day.
European Food Safety Administration believes health
risk for all population group is low because “estimates
for...exposure...are 3-5 times lower than the proposed t-
TDI”
Regulatory/Legal: United
States No current TDI as defined by the FDA- current
assessment is that BPA is safe at “the very low levels that occur in some foods”
Some studies have been initiated by the National Center for Toxicological Research; findings will be published in peer-reviewed scientific literature.
Have published a rule amending food additive regulations to no longer provide for the use of BPA based epoxy resins as coatings in infant formula packaging because this use has been abandoned.(2013)
Regulatory/Legal: States California, Maine, Maryland, Massach
usetts, New York, Iowa, Minnesota, Connecticut, and Wisconsin have banned BPA in all baby bottles and sippy cups.
Washington and Vermont have both banned it in all sports bottles, reusable food/beverage containers, as well as baby bottles and sippy cups
Colorado vetoed House Bill 12-1174 in February 2012, which proposed to ban BPA in baby bottles and sippy cups.
Risk Characterization:
Prostate Cancer Prostate cancer is the second leading cause of cancer-
related death in U.S. men
Approximately 15% of men will be diagnosed with prostate cancer in their lifetime
With the 1987 introduction of prostate-specific antigen testing, the newly enhanced ability to diagnose the disease caused incidence to spike to 240 age-adjusted cases per 100,000 men by 1992. After this “catch-up” period rates dropped for three years, but are now back on the rise.
•Previous research has linked elevated estrogen levels during pregnancy to increased risk of prostate cancer in males.
Risk Characterization:
Prostate Cancer BPA is a suspected endocrine disruptor
Acts by interfering with the biosynthesis, secretion, action
or metabolism of naturally occurring hormones.
In animal models, estrogens can drive carcinogenesis of
the prostate and have long been suspected of playing a
role in human prostate cancer
Scientists have hypothesized that prenatal exposure to
estrogen-like compounds including BPA (monomeric
bisphenol A) may account for recent increases in the
rates of prostate cancer.
Prostate Cancer and BPA:
Centromeres
Centromere region is where sister
chromatids are attached
During mitosis/cell division spindle
poles will adhere to the centromere
region
Centromeres have dynamic
assemblies of chromatin and have
specialized functional regions
Centromeres: Chromosomal
Regions Essential to Mitosis/Cell
Division
Prostate Cancer:
Study Suggests BPA interacts with several different receptors and can
act as an artificial estrogen
Urinary BPA levels are associated with PCa and may
have prognostic value
BPA exposure can disrupt mitosis/cell division;
specifically acting on centromeres
BPA exposure may be correlated with prostate cancer
carinogenesis
PLOS (Public Library of Science) Exposure to B
Study Validity and Reliability Research was done with animal studies and cell based
model studies
This can make it difficult to extrapolate to humans and
results should be seen as preliminary findings
Obviously, important to replicate study results and more
needs to happen with this
BPA and Possible Effects
On Mitosis Low doses of BPA promoted centrosome
amplification/altering of the centromere
Low doses of BPA had an adverse effect on
centrosome numbers
Number of centrosomes per cell were scored by
fluorescence microscopy
Cells with abnormal centromeres increased
Suggest BPA may disrupt the centromere’s job and long
term play a role in prostate carcinogenesis
BPA as an estrogen Low level exposure elicited Cancer with the greatest
effect
This observation supports findings in other literature
regarding interaction with receptors to affect estrogen
levels. High estrogen levels are correlated with PCa.
Urinary BPA Levels May show Correlation
with Prostate Cancer
Stratified analyses showed
the association between
urinary BPA levels and
Prostate Cancer was highly
significant among patients <
65 and that it was not
significant for those >65.
Perplexing- suggests that higher BPA exposure is associated
with earlier onset of Prostate Cancer. However, based on theory
of developmental reprogramming of cancer risk the findings do
raise the possibility of early life reprogramming of Prostate
Cancer in humans
BPA Exposure correlated with Prostate
Carcinogenesis
Chronic BPA exposure
promotes independent
abnormal growth in cells
Representative of colonies
after 2 weeks incubation
Cells with the BPA exposure
formed larger colonies
compared with those grown
in absence of BPA
Study Conclusions “We know that stem cells help replenish our organs
throughout life. We propose that if there is exposure
early in life to an estrogenic compound- BPA- it
reprograms our stem cells,” say Gail Prins, a University
of Illinois, Chicago researcher of the study (published in
Endocrinology)
This could be the latest addition to the growing field of
epigenetics, linking this chemical to altered DNA in
fetuses and the potential for later life disease.
Concerns The effects of low-dose exposure to BPA in lab animals
are not always reproducible.
Need to exercise caution when extrapolating these findings to humans; the study was derived from animal studies and cell based models.
How could an analogous study on men be done?
To obtain results of early exposure to BPA and it’s relation to prostate cancer would take 50 plus years
More research is needed- but does this justify holding out on a ban on BPA?
American Chemistry Council News Release
For Immediate Release - Washington (Jan. 6, 2014)
STUDY CLAIMING INCREASED PROSTATE CANCER
RISK FROM BPA EXPOSURE IS NOT SUPPORTED
BY RELIABLE HUMAN EXPOSURE DATA
“The weight of scientific evidence on BPA has been
extensively evaluated by government and scientific
bodies around the world, which have declared it safe
as used in food contact materials.”