antibody cross-reactivity information non-human primate cells€¦ · rhesus monkey macaca mulatta...

Antibody cross-reactivity information - NHP cells May 2018 Copyright © 2018 Miltenyi Biotec Inc. All rights Reserved1

Moreover, most of the Miltenyi Biotec antibodies are tested to cross-react with other non-human primates, for example cynomolgus monkey (Macaca fascicularis). Please visit www.miltenyibiotec.com/nhp-antibodies for detailed information.

The MACS® Antibodies for non-human primate cells have excellent performance for the high-quality detection and analysis of cells from the rhesus monkey (Macaca mulatta), an important animal model in non-human primate research.

Antibody Clone Non-human primate species Other species

α-Actinin (Sarcomeric) REA402

Sheep, rabit, lizard, feline, canine,

snake, fish, goat, chicken, bovine,

frog, pig, hamster

Anti-Ig λ Light Chain IS7-24C7

Anti-IgA IS11-21E11 No

Anti-IgE MB10-5C4

APCDD1 REA887

CCL2 (MCP-1) REA248

CCL2 (MCP-1) REA485

CCL5 (RANTES) REA346

CD1c (BDCA-1) AD5-8E7 7

CD1c (BDCA-1) REA694

CD2 LT2 1 7 1

CD2 REA972

Rhes

us m

onke

yM

acac

a m

ulat

ta

Cyno

mol

gus

mon

key

Mac

aca

fasc

icul

aris

Pigt

ail m

onke

yM

acac

a ne

mes

trin

a

Chim

panz

eePa

n tr

oglo

dyte

s

Babo

onPa

pio

anub

is

Cott

onto

p ta

mar

inSa

ngui

nus o

edip

us

Caup

uchi

n m

onke

yCe

bus a

pella

nig

ritus

Afr

ican

gre

en m

onke

yCh

loro

cebu

s aet

hiop

s

Com

mon

mar

mos

etCa

llith

rix ja

cchu

s

Ham

adry

as B

aboo

nPa

pio

ham

adry

as

Soot

y m

anga

bey

Cero

cebu

s aty

s

Squi

rrel

mon

key

Saim

iri sc

iure

us

Bon

obo

chim

panz

eePa

n pa

nisc

us

Ow

l mon

key

Aotu

s triv

irgat

us

* Weak staining Tested to be reactive No: Tested to be non-reactive REAfinity™ Recombinant Antibody

Antibody cross-reactivity information

Non-human primate cells




CD3 10D12 No 7 No

CD3 OKT3 No Horse6

CD3 REA994

CD4 M-T466 7 Horse6

CD5 UCHT2 1

CD6 M-T411

CD8 BW135/80 7 No

CD8 BW135/80 Fab

CD11a REA378 Horse, cattle

(bovine, cow), sheep, dog, rabbit

CD11b M1/70.15.11.5 7 Horse5

CD11b REA592

CD14 TUK4 2 7

Pig, cow, sheep, goat, dog, mink,

rabbit3

CD16 REA423

CD16 VEP13 No 7

CD20 LT20 7 No

CD21 HB5

CD25 4E3 7

CD25 3G10 No

CD25 REA945

CD27 M-T271 4

CD28 15E8 7 No

CD31 AC128 No

CD31 REA1028

CD32 2E1 4 Horse5,6

CD32 REA997 Horse

CD33 AC104.3E3

CD34 AC136 No Horse5,6

CD35 E11

CD36 AC106 Horse5,6

CD39 MZ18-23C8

CD40 HB14

CD40 REA733

CD41a REA386

CD43 REA833

CD44 DB105

CD44 IM7.8.1 Pig, cow, horse,

cat, dog

CD44 REA690

CD45 MB4-6D6 No No

Rhes

us m

onke

yM

acac

a m

ulat

ta

Cyno

mol

gus

mon

key

Mac

aca

fasc

icul

aris

Pigt

ail m

onke

yM

acac

a ne

mes

trin

a

Chim

panz

eePa

n tr

oglo

dyte

s

Babo

onPa

pio

anub

is

Cott

onto

p ta

mar

inSa

ngui

nus o

edip

us

Caup

uchi

n m

onke

yCe

bus a

pella

nig

ritus

Afr

ican

gre

en m

onke

yCh

loro

cebu

s aet

hiop

s

Com

mon

mar

mos

etCa

llith

rix ja

cchu

s

Ham

adry

as B

aboo

nPa

pio

ham

adry

as

Soot

y m

anga

bey

Cero

cebu

s aty

s

Squi

rrel

mon

key

Saim

iri sc

iure

us

Bon

obo

chim

panz

eePa

n pa

nisc

us

Ow

l mon

key

Aotu

s triv

irgat

us




CD45 REA1023

CD45R (B220) RA3-6B2 Horse5

CD45RA T6D11

CD45RB REA119

CD45RO REA611 No

CD45RO UCHL1 No * No Trout, carp

CD49d MZ18-24A9

CD49d REA545 Horse, cattle

(bovine, cow), sheep, dog, cat

CD49e NKI-SAM1

CD49e REA686

CD49f GoH3 * 4 Pig, dog

CD49f REA518 Pig, dog

CD56 AF12-7H3

CD56 REA196

CD59 REA496

CD61 Y2/51 No

CD62L REA615 No

CD62P REA389

CD63 H5C6

CD63 REA1055

CD64 10.1.1 * 4

CD64 REA978

CD66abce TET2

CD68 REA886

CD68 Y1/82A

CD69 FN50 4 7

CD69 REA824

CD73 AD2 *

CD73 REA804

CD79a HM47

CD79b REA120 No No

CD80 2D10

CD80 REA661

CD81 EAT2

CD81 REA513

CD83 HB15 * 4 No Horse5,6

CD86 FM95

CD86 REA968

CD87 VIM5

CD90 REA897

Rhes

us m

onke

yM

acac

a m

ulat

ta

Cyno

mol

gus

mon

key

Mac

aca

fasc

icul

aris

Pigt

ail m

onke

yM

acac

a ne

mes

trin

a

Chim

panz

eePa

n tr

oglo

dyte

s

Babo

onPa

pio

anub

is

Cott

onto

p ta

mar

inSa

ngui

nus o

edip

us

Caup

uchi

n m

onke

yCe

bus a

pella

nig

ritus

Afr

ican

gre

en m

onke

yCh

loro

cebu

s aet

hiop

s

Com

mon

mar

mos

etCa

llith

rix ja

cchu

s

Ham

adry

as B

aboo

nPa

pio

ham

adry

as

Soot

y m

anga

bey

Cero

cebu

s aty

s

Squi

rrel

mon

key

Saim

iri sc

iure

us

Bon

obo

chim

panz

eePa

n pa

nisc

us

Ow

l mon

key

Aotu

s triv

irgat

us



CD93 VIMD2

CD95 DX2 4 7

CD95 REA738

CD98 REA387

CD100 REA316

CD107a H4A3 * 4 7

CD107b H4B4

CD123 REA918

CD123 (IL-3Ra) AC145 No

CD127 MB15-18C9

(low)No

CD137 4.1BB No No No

CD137 4B4-1 4

CD137 REA765

CD138 44F9 No No

CD138 B-B4 No No Horse5,6

CD150 REA151

CD152 BNI3 * 4

CD152 REA1003

CD154 5C8 7

CD154 REA238

CD156 REA331 No

CD159a (NKG2A) REA110

CD163 GHI/61.1

(low)

CD163 REA812

CD165 SN2

CD170 (Siglec-5) 1A5

CD171 REA163

CD177 REA258

CD180 (RP105) MHR73-11

CD180 (RP105) REA956

CD184 (CXCR4) 12G5

CD184 (CXCR4) REA649

CD197 (CCR7) REA546 No

CD205 HD30

CD212 REA242

CD222 REA187

CD226 (DNAM-1) DX11

CD226 (DNAM-1) REA1040

Rhes

us m

onke

yM

acac

a m

ulat

ta

Cyno

mol

gus

mon

key

Mac

aca

fasc

icul

aris

Pigt

ail m

onke

yM

acac

a ne

mes

trin

a

Chim

panz

eePa

n tr

oglo

dyte

s

Babo

onPa

pio

anub

is

Cott

onto

p ta

mar

inSa

ngui

nus o

edip

us

Caup

uchi

n m

onke

yCe

bus a

pella

nig

ritus

Afr

ican

gre

en m

onke

yCh

loro

cebu

s aet

hiop

s

Com

mon

mar

mos

etCa

llith

rix ja

cchu

s

Ham

adry

as B

aboo

nPa

pio

ham

adry

as

Soot

y m

anga

bey

Cero

cebu

s aty

s

Squi

rrel

mon

key

Saim

iri sc

iure

us

Bon

obo

chim

panz

eePa

n pa

nisc

us

Ow

l mon

key

Aotu

s triv

irgat

us




CD271 (LNGFR) ME20.4-1.H4Goat, dog, pig,

sheep, cat, rabbit, raccoon8

CD271 (LNGFR) REA648 Guinea pig

CD271 (LNGFR) REA844Monkey, sheep,

cat, dog, pig, rabbit

CD279 (PD-1) PD1.3.1.3

CD283 TLR 3.7

CD284 HTA125 Guinea pig

CD303 (BDCA-2) AC144 No * No 7 No

CD303 (BDCA-2) REA693

CD314 (NKG2D) BAT221 9

CD314 (NKG2D) REA797

CD317 REA202

CD319 REA150

CD337 (NKp30) AF29-4D12

(low)

CD366 (TIM-3) REA635

CD434 DF-T1

CX3CR-1 REA385

FoxP3 3G3 * No

GM-CSF BVD2-21C11

HLA-ABC REA230

HLA-DR AC122 Guinea pig

HLA-DR REA805

HLA-DR, DP, DQ REA332

IFN-α LT27:295 7

IFN-γ 45-15

IL-1α 364-3B3-14

IL-2 N7.48A 7

IL-4 7A3-3

IL-4 REA895 Pig

IL-5 JES1-39D10

IL-5 REA1025

IL-10 JES3-9D7 No

IL-10 REA842

IL-12 (p40/p70) C8.6

IL-13 JES10-5A2.2

IL-17A CZ8-23G1

iNKT 6B11

KIR2D NKVFS1 No

KIR2D REA1042

Rhes

us m

onke

yM

acac

a m

ulat

ta

Cyno

mol

gus

mon

key

Mac

aca

fasc

icul

aris

Pigt

ail m

onke

yM

acac

a ne

mes

trin

a

Chim

panz

eePa

n tr

oglo

dyte

s

Babo

onPa

pio

anub

is

Cott

onto

p ta

mar

inSa

ngui

nus o

edip

us

Caup

uchi

n m

onke

yCe

bus a

pella

nig

ritus

Afr

ican

gre

en m

onke

yCh

loro

cebu

s aet

hiop

s

Com

mon

mar

mos

etCa

llith

rix ja

cchu

s

Ham

adry

as B

aboo

nPa

pio

ham

adry

as

Soot

y m

anga

bey

Cero

cebu

s aty

s

Squi

rrel

mon

key

Saim

iri sc

iure

us

Bon

obo

chim

panz

eePa

n pa

nisc

us

Ow

l mon

key

Aotu

s triv

irgat

us

* Weak staining Tested to be reactive No: Tested to be non-reactive



MSCA-1 REA781 Dog, pig, sheep,

goat, monkey

MSCA-1 W8B2 Dog, sheep, pig,

goat

NKp80 4A4.D10

NKp80 REA845

p120 Catenin pS288 REA498Mouse, monkey,

dog

PCNA REA858

Chicken, rabbit, hamster, horse, sheep, dog, cat, ferret, xenopus

TCR Vb5 REA910

TCR Vγ9 REA470

TOM22 1C9-2

Rhes

us m

onke

yM

acac

a m

ulat

ta

Cyno

mol

gus

mon

key

Mac

aca

fasc

icul

aris

Pigt

ail m

onke

yM

acac

a ne

mes

trin

a

Chim

panz

eePa

n tr

oglo

dyte

s

Babo

onPa

pio

anub

is

Cott

onto

p ta

mar

inSa

ngui

nus o

edip

us

Caup

uchi

n m

onke

yCe

bus a

pella

nig

ritus

Afr

ican

gre

en m

onke

yCh

loro

cebu

s aet

hiop

s

Com

mon

mar

mos

etCa

llith

rix ja

cchu

s

Ham

adry

as B

aboo

nPa

pio

ham

adry

as

Soot

y m

anga

bey

Cero

cebu

s aty

s

Squi

rrel

mon

key

Saim

iri sc

iure

us

Bon

obo

chim

panz

eePa

n pa

nisc

us

Ow

l mon

key

Aotu

s triv

irgat

us

1. Ozwara et al. (1997) J. Med. Primatol. 26: 164–171.2. Wilson et al. (1995) J. Immunol. Meth. 178: 195–200.3. Jacobsen et al. (1993) Vet. Immunol. Immunopathol. 39: 461–466.4. Lin et al. (2002) Leucocyte Typing VII, 519–524.5. Steinbach et al. (2007) Vet. Immunol. Immunopathol. 119: 100–105.6. Ibrahim and Steinbach (2007) Vet. Immunol. Immunopathol. 119: 81–91.7. Information provided by Dr. D. Kunkel, Institut Pasteur, Paris.8. Tremere et al. (2000) Neuroreport 11: 2177–2183.9. Mavilio et al. (2005) Blood 166: 1718–1725.

References

Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact.

MACS is a registered trademarks of Miltenyi Biotec GmbH. Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.

* Weak staining Tested to be reactive No: Tested to be non-reactive

antibody cross-reactivity information non-human primate cells€¦ · rhesus monkey macaca mulatta...

Documents