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Technical Note Analysis of Sugars in Honey and Syrup Analysis of honey and syrup is an important part of detecting food adulteration. Sugar-D provides good and consistent separation with high durability. Standards Honey COSMOSIL Application Data NACALAI TESQUE, INC Column: COSMOSIL Sugar-D Column size: 4.6mmI.D.-250mm Mobile phase: Acetonitrile/ H 2 O = 75/25 Flow rate: 1.0 ml/min Temperature: 30°C Detection: RI Sample: 1; Fructose (30mg/ml) 2; Glucose (24mg/ml) 3; Sucrose (6mg/ml) 4; Maltose (6mg/ml) Inj.Vol.: 5.0µl COSMOSIL Application Data NACALAI TESQUE, INC Column: COSMOSIL Sugar-D Column size: 4.6mmI.D.-250mm Mobile phase: Acetonitrile/ H 2 O = 75/25 Flow rate: 1.0 ml/min Temperature: 30°C Detection: RI Sample: Honey (50mg/ml) 1; Fructose 2; Glucose Inj.Vol.: 5.0µl Gum syrup Gum syrup (contains honey) COSMOSIL Application Data NACALAI TESQUE, INC Column: COSMOSIL Sugar-D Column size: 4.6mmI.D.-250mm Mobile phase: Acetonitrile/ H 2 O = 75/25 Flow rate: 1.0 ml/min Temperature: 30°C Detection: RI Sample: Gum syrup (50mg/ml) 1; Fructose 2; Glucose Inj.Vol.: 5.0µl COSMOSIL Application Data NACALAI TESQUE, INC Column: COSMOSIL Sugar-D Column size: 4.6mmI.D.-250mm Mobile phase: Acetonitrile/ H 2 O = 75/25 Flow rate: 1.0 ml/min Temperature: 30°C Detection: RI Sample: Gum syrup (contains honey) (50mg/ml) 1; Fructose 2; Glucose Inj.Vol.: 5.0µl Maple syrup Sugar syrup (Maple flavor) COSMOSIL Application Data NACALAI TESQUE, INC Column: COSMOSIL Sugar-D Column size: 4.6mmI.D.-250mm Mobile phase: Acetonitrile/ H 2 O = 75/25 Flow rate: 1.0 ml/min Temperature: 30°C Detection: RI Sample: Maple syrup (50mg/ml) 1; Fructose 2; Glucose 3; Sucrose Inj.Vol.: 5.0µl COSMOSIL Application Data NACALAI TESQUE, INC Column: COSMOSIL Sugar-D Column size: 4.6mmI.D.-250mm Mobile phase: Acetonitrile/ H 2 O = 75/25 Flow rate: 1.0 ml/min Temperature: 30°C Detection: RI Sample: Sugar syrup (Maple flavor) (50mg/ml) 1; Fructose 2; Glucose 3; Sucrose Inj.Vol.: 5.0µl

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Page 1: Analysis of Sugars in Honey and Syrup - nacalai.co.jp · Technical ote Analysis of Sugars in Honey and Syrup Analysis of honey and syrup is an important part of detecting food adulteration

Technical Note

Analysis of Sugars in Honey and Syrup

Analysis of honey and syrup is an important part of detecting food adulteration. Sugar-D provides good and consistent separation with high durability.

Standards Honey

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: 1; Fructose (30mg/ml)2; Glucose (24mg/ml)3; Sucrose (6mg/ml)4; Maltose (6mg/ml)

Inj.Vol.: 5.0µl

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Honey (50mg/ml)1; Fructose2; Glucose

Inj.Vol.: 5.0µl

Gum syrup Gum syrup (contains honey)

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Gum syrup (50mg/ml)1; Fructose2; Glucose

Inj.Vol.: 5.0µl

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Gum syrup (contains honey)(50mg/ml)1; Fructose2; Glucose

Inj.Vol.: 5.0µl

Maple syrup Sugar syrup (Maple flavor)

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Maple syrup (50mg/ml)1; Fructose2; Glucose 3; Sucrose

Inj.Vol.: 5.0µl

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Sugar syrup (Maple flavor)(50mg/ml)1; Fructose2; Glucose 3; Sucrose

Inj.Vol.: 5.0µl

Page 2: Analysis of Sugars in Honey and Syrup - nacalai.co.jp · Technical ote Analysis of Sugars in Honey and Syrup Analysis of honey and syrup is an important part of detecting food adulteration

1701

For research use only, not intended for diagnostic or drug use.

NACALAI TESQUE, INC.Nijo Karasuma, Nakagyo-ku, Kyoto 604-0855 JAPANTELFAXWebsiteE-mail

: +81-(0)75-251-1730: +81-(0)75-251-1763: www.nacalai.com: [email protected]

Apple syrup Agave syrup

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Apple syrup (50mg/ml)1; Fructose2; Glucose

Inj.Vol.: 5.0µl

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Agave syrup (50mg/ml)1; Fructose2; Glucose

Inj.Vol.: 5.0µl

Starch syrup Starch syrup (fast analysis)

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 75/25Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Starch syrup (50mg/ml)1; Glucose2; Maltose3; Maltotriose

Inj.Vol.: 5.0µl

COSMOSIL Application Data

NACALAI TESQUE, INC

Column: COSMOSIL Sugar-DColumn size: 4.6mmI.D.-250mmMobile phase: Acetonitrile/ H2O = 60/40Flow rate: 1.0 ml/min Temperature: 30°CDetection: RI

Sample: Starch syrup (50mg/ml)1; Glucose2; Maltose3; Maltotriose

Inj.Vol.: 5.0µl

More Sugar-D application data are available on our website (http://www.nacalai.co.jp/ cosmosil/data/csmosrchtop.cfm). Or you can search “COSMOSIL Application”.

DurabilityThe decrease of retention time was compared between COSMOSIL Sugar-D and conventional aminopropyl bonded stationaryphase under severe condition of 100% water as eluent between tests. The capacity factor did not decrease in case to COSMOSIL Sugar-D.

[Test Condition]Column SizeMobile PhaseFlow RateTemperatureDetectionSample

4.6 mm I.D. x 250 mmAcetonitrile : Water = 70 : 301.0 ml/min30RIMaltose

[Eluent Condition]EluentFlow RateTemperature

Water1.0 ml/minRT

120%

0%

100%

80%

60%

40%

20%

0 12010080604020Time (hr)

Sugar-DAminopropyl Type


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