short term exercise ameliorates metabolic impact of maternal obesity in offspring

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mimarkers caused by maternal obesity and postnatalover-nutrition.

http://dx.doi.org/10.1016/j.orcp.2012.08.042

20

reduced expression of both genes in subcutaneousadipose tissue (P < 0.05).

Conclusion: The data showed tissue specific DNAmethylation and gene expression of PGC-1� andIGF2. PGC-1� DNA methylation may play a keyrole in its expression, at least in muscle. More-over, altered DNA methylation and expression ofboth genes may also be associated with obesityand T2DM. However, further analysis with a largercohort is required.

http://dx.doi.org/10.1016/j.orcp.2012.08.040

O039

Foods for appetite control: Developing plant-based functional foods targeting satiety

J. Ingram 1,∗, K. Sutton 2, K. Lo 1, E. Walker 1, S.Parkar 3, J. Ansell 4, J. Monro 4, L. Huffman 4, S.Poppitt 5

1 The New Zealand Institute for Plant & FoodResearch, Auckland, New Zealand2 The New Zealand Institute for Plant & FoodResearch, Lincoln, New Zealand3 The New Zealand Institute for Plant & FoodResearch, Hamilton, New Zealand4 The New Zealand Institute for Plant & FoodResearch, Palmerston North, New Zealand5 Human Nutrition Unit, School of Biological Sci-ences, The University of Auckland, Auckland, NewZealand

Aim: The chemo-sensory mechanisms of the gutepithelium, particularly those involved in detectingand relaying to the brain the chemical composi-tion of food during digestion, play important rolesin controlling gut function and appetite regula-tion. We aim to develop plant-based foods and foodingredients that are able to enhance and extendsatiety by applying a ‘‘combinatorial’’ approach,targeting specific chemosensory mechanisms in theproximal small intestine (duodenum), the distalsmall intestine (ileum) and the colon that are acti-vated at different times post meal.

Method: A wide range of methods includingin vitro models of gastric, small intestine andcolonic digestion, enzymatic and cell-based screen-ing assays, and consumer and clinical studies involunteer participants are been used to investi-gate each aspect of the proposed combinatorialapproach to enhance satiety.

Results: The mechanisms to be presentedinclude (1) duodenal stimulation of the ‘satiety’

Oral Abstracts

he ileum, and (3) prebiotic stimulation of colonicicrobial metabolite production.Conclusion: We will provide an overview of the

esearch programme ‘‘Foods for Appetite Con-rol’’ including the rational behind the differenthemosensory mechanisms selected and details ofur plant bioactive screening approaches.

ttp://dx.doi.org/10.1016/j.orcp.2012.08.041

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hort term exercise ameliorates metabolicmpact of maternal obesity in offspring

. Morris ∗, H. Bahari, V. Caruso

University of New South Wales, Sydney, Australia

Aim: Maternal obesity is an emerging globalealth issue. This study aimed to systematicallyxamine the relationship between maternal obe-ity and subsequent offspring metabolic disease andhether this can be ameliorated by short term exer-ise implemented late in life in the female offspringf obese rats mothers.

Method: Adult female Sprague Dawley rats wereed either normal chow or high fat diet (HFD) adibitum for 5 weeks to yield chow and HFD mothers.ams consumed the same diet throughout gestationnd lactation. At weaning, female rats were sepa-ated into two diet groups, chow (C) or HFD (F), andfter 7 weeks on their respective diet, half of eachroup were exercised (voluntary running wheels)or 5 weeks while the remainder were sedentary.ats were killed at 15 weeks for blood and brainhypothalamus) collection.

Results: Voluntary exercise for 5 weeks sig-ificantly decreased body weight, RpWAT mass17% and 35% decrease respectively), plasma leptinnd insulin concentrations in offspring of F moth-rs consuming HFD compared to sedentary ratsall P < 0.05). Hypothalamic FTO mRNA expressionas significantly increased (P < 0.05) due to post-eaning HFD in offspring from F mothers, and wasecreased by exercise (P < 0.01).

Conclusion: A short period of exercise imple-ented post puberty had beneficial effects in

mproving the phenotypic profile and hypothalamic