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mimarkers caused by maternal obesity and postnatalover-nutrition.

http://dx.doi.org/10.1016/j.orcp.2012.08.042

20

reduced expression of both genes in subcutaneousadipose tissue (P < 0.05).

Conclusion: The data showed tissue specific DNAmethylation and gene expression of PGC-1� andIGF2. PGC-1� DNA methylation may play a keyrole in its expression, at least in muscle. More-over, altered DNA methylation and expression ofboth genes may also be associated with obesityand T2DM. However, further analysis with a largercohort is required.

http://dx.doi.org/10.1016/j.orcp.2012.08.040

O039

Foods for appetite control: Developing plant-based functional foods targeting satiety

J. Ingram 1,∗, K. Sutton 2, K. Lo 1, E. Walker 1, S.Parkar 3, J. Ansell 4, J. Monro 4, L. Huffman 4, S.Poppitt 5

1 The New Zealand Institute for Plant & FoodResearch, Auckland, New Zealand2 The New Zealand Institute for Plant & FoodResearch, Lincoln, New Zealand3 The New Zealand Institute for Plant & FoodResearch, Hamilton, New Zealand4 The New Zealand Institute for Plant & FoodResearch, Palmerston North, New Zealand5 Human Nutrition Unit, School of Biological Sci-ences, The University of Auckland, Auckland, NewZealand

Aim: The chemo-sensory mechanisms of the gutepithelium, particularly those involved in detectingand relaying to the brain the chemical composi-tion of food during digestion, play important rolesin controlling gut function and appetite regula-tion. We aim to develop plant-based foods and foodingredients that are able to enhance and extendsatiety by applying a ‘‘combinatorial’’ approach,targeting specific chemosensory mechanisms in theproximal small intestine (duodenum), the distalsmall intestine (ileum) and the colon that are acti-vated at different times post meal.

Method: A wide range of methods includingin vitro models of gastric, small intestine andcolonic digestion, enzymatic and cell-based screen-ing assays, and consumer and clinical studies involunteer participants are been used to investi-gate each aspect of the proposed combinatorialapproach to enhance satiety.

Results: The mechanisms to be presentedinclude (1) duodenal stimulation of the ‘satiety’

peptide cholecystokinin (CCK) release using bitterphytochemicals, (2) activation of the ileal brakemechanism through enhanced delivery of CHO to

Oral Abstracts

he ileum, and (3) prebiotic stimulation of colonicicrobial metabolite production.Conclusion: We will provide an overview of the

esearch programme ‘‘Foods for Appetite Con-rol’’ including the rational behind the differenthemosensory mechanisms selected and details ofur plant bioactive screening approaches.

ttp://dx.doi.org/10.1016/j.orcp.2012.08.041

040

hort term exercise ameliorates metabolicmpact of maternal obesity in offspring

. Morris ∗, H. Bahari, V. Caruso

University of New South Wales, Sydney, Australia

Aim: Maternal obesity is an emerging globalealth issue. This study aimed to systematicallyxamine the relationship between maternal obe-ity and subsequent offspring metabolic disease andhether this can be ameliorated by short term exer-ise implemented late in life in the female offspringf obese rats mothers.

Method: Adult female Sprague Dawley rats wereed either normal chow or high fat diet (HFD) adibitum for 5 weeks to yield chow and HFD mothers.ams consumed the same diet throughout gestationnd lactation. At weaning, female rats were sepa-ated into two diet groups, chow (C) or HFD (F), andfter 7 weeks on their respective diet, half of eachroup were exercised (voluntary running wheels)or 5 weeks while the remainder were sedentary.ats were killed at 15 weeks for blood and brainhypothalamus) collection.

Results: Voluntary exercise for 5 weeks sig-ificantly decreased body weight, RpWAT mass17% and 35% decrease respectively), plasma leptinnd insulin concentrations in offspring of F moth-rs consuming HFD compared to sedentary ratsall P < 0.05). Hypothalamic FTO mRNA expressionas significantly increased (P < 0.05) due to post-eaning HFD in offspring from F mothers, and wasecreased by exercise (P < 0.01).

Conclusion: A short period of exercise imple-ented post puberty had beneficial effects in

mproving the phenotypic profile and hypothalamic