7th annual newsletter of barley improvement through ......park field station. they are evaluated for...

7th Annual Newsletter of barley improvement through germplasm:

co-ordination, introduction and evaluation. Contact: Dr. Tefera T. Angessa The University of Western Australia, Shenton Park Field Station, 1 Underwood Avenue, Shenton Park, WA 6008 [email protected] Ph (08) 9387 3646 Fax (08) 9383 9907

7th Annual Newsletter of UWA-GRDC Barley Germplasm Program 1

Acknowledgements The financial support by the Grains Research and Development Corporation (GRDC) is highly appreciated. The tremendous works of Ms Christina Grime (20% FTE), who has been in charge of barley germplasm program for more than a decade and is leaving by the end of June 2009, has been exceptional and fruitful. I am very grateful to her assistance during my first year of taking over the position she is leaving. The unreserved effort of other barley germplasm program’s staffs: Ms Christine Munday (30% FTE) also for proof reading this newsletter and Ms Rosemarie Lugg (30% FTE) is highly appreciated. Dr. Rodger Boyd’s keenness to share his immense experience and availability to help is greatly acknowledged. The UWA/GRDC’s barley germplasm program has been playing a pivotal role in the barley industry of Australia. It established several collaborators nationally and internationally. This includes Dr. Reg Lance, Dr. Chengdao Li, Ms Cathy Burchell, Ms Sue Broughton, Dr. Sanjiv Gupta and Mr Jason Bradley from DAFWA; Dr. Jerome Franckowiack and Mr Greg Platz from QDPI&F; Dr. Jason Eglinton, Dr. Stewart Coventry and Ms Amanda Box from Adelaide University; Dr. Hugh Wallwork and Mr Mark Butt from SARDI; Dr. Mark McLean and Dr. David Moody from VIC-DPI; Dr. Meixue Zhou from Tasmania Institute of Agriculture Research and Mr Greg Grimes from AWCC, Tamworth NSW. I am very grateful to all the people and institutions involved in this program. I also owe my sincere gratitude to our overseas collaborators for their collaboration and provision of germplasm making the program’s goals achievable.


Table of contents

Content Page

1. Background 4

2. New introductions 5

3. Seed bulking 5

4. Evaluation

4.1 Screening for disease resistance 6

4.1.1 Scald 6

4.1.2 Spot form net blotch 7

4.1.3 Powdery mildew 7

4.1.4 Collaborative work

4.1.4.1 Barley Leaf Rust 8

4.1.4.2 Other diseases 8

4.2 Photoperiod response and basic vegetative period 9

4.3 Boron toxicity 10

4.4 Previously enhanced lines 11

5. Appendixes 12

6. Reference 23


Abbreviations

Abbreviation Description AWCC Australian Winter Cereals Collection BBA Barley Breeding Australia BLR Barley leaf rust BLR Barley leaf rust BVP Basic vegetative period DAFWA Department of Food and Agriculture Western Australia EBC European Brewing Convention EBDSN Elite barley disease screening nursery Fig. Figure GRDC Grains Research and Development Corporation INIA Instituto Nacional de Investigacion Agropecuaria, Uruguay LAD Leaf area damage NFNB Net form net blotch No Number PM Powdery mildew ppm parts per million QDPI&F Queensland Department of Primary Industries and fisheries SABBI South African Barley Breeding Institute SARDI South Australian Research and Development Institute SC Scald SFNB Spot form net blotch UWA University of Western Australia VIC-DPI Department of Primary Industries of Victoria WCC Western Canadian Co-operative


1. Background Diverse germplasm is the backbone of sustainable agriculture for present and future generations. Barley, the current and future improvement of which is dependent on diverse germplasm, is one of the most important cereal crops globally. It is playing a pivotal role as a malting and feed commodity in domestic and international markets. The “Barley improvement through germplasm: co-ordination, introduction and evaluation” program of the University of Western Australia (UWA) funded by the Grains Research and Development Corporation (GRDC) is playing a key role in making diverse germplasm available to current barley breeding undertakings. Since its inception in the 1960s, the program has been supporting various improvement programs through harnessing germplasm conservation with breeding works. The overall goal of the project is to improve current varieties through Barley Breeding Australia (BBA), http://www.barleybreedingaustralia.com.au/, using novel traits from diverse sources while also contributing to safeguarding barley genetic diversity. To facilitate this, germplasm of interest for various traits are imported from different countries and regions based on requests from BBA and other barley researchers across Australia. Various international, regional and local publications are also used to trace germplasm, which may be of importance to the barley industry of Australia. This newsletter summarizes various activities undertaken within the UWA/GRDC barley project for the period of July 2008 to June 2009. Barley germplams from many different seed provenances are imported and quarantined at UWA’s Shenton Park Field Station post-entry quarantine facility. Seeds from this material, which will be called accessions in this newsletter, regardless of their level of progression in any breeding program, once released from quarantine, are bulked in the following winter at Shenton Park Field Station. They are evaluated for various agronomic and traits of interest under various conditions at Shenton Park Field station. The preliminary data is then collated and made available to appropriate parties. In the second season accessions evaluations combines with screened for their resistance to various diseases, which including scald, spot form of net blotch (SFNB) and powdery mildew in winter under field condition. Seedling test to boron toxicity tolerance is carried out in summer/Autumn at the Shenton Park Field Station. Accessions with convincing level of disease resistance are further tested by plant pathology groups across Australia. Those with confirmed resistance levels and others with important agronomic or quality traits indicated by these evaluations are made available for trial in other environments used in on-going improvement programs. All accessions are retained in our collections so they are available for future uses. The UWA-GRDC germplasm collection has more than 12,000 accessions, which include released cultivars, landraces, advanced breeding lines and wild lines from various sources. It has also more than 40,000 DH populations mainly from DAFWA. The program continues to strive for the global safeguarding of barley germplasm diversity in coordination with Australian Winter Cereal Collection (AWCC) based at Tamworth, NSW. As a result, all introductions are assigned unique 5 digit UWA numbers and kept for long term storage and future use at the UWA-GRDC’s barley collection. Most seeds are available to interested parties for research purposes in limited amount. The program recognizes and respects the importance of Material Transfer Agreement (MTA) attached to some of the germplasm handled within the project. It requests all parties receiving seeds from the program to apply the conditions attached and accompanying to them and anticipates they will do so.


2. New introductions More than 800 accessions, which include advanced breeding lines, cultivars and landraces, of barley germplasm were introduced from overseas in the period of 2007-2009 (Table 1). They are at various stages with respect to their release from quarantine. There is a regular exchange program between the UWA-Canada and UWA-South Africa which is a very effective and timely way of germplasm introduction. To broaden access to germplasm internationally, further long-lasting collaborations and exchange programs need to be established. Table 1. Germplasm introduced in 2008/2009.

Country (donor) No. Notes

Canada (Prof. B. Rossnagel) 73

Western Canadian Co-operative’s (WCC) elite breeder’s lines. 41 Released from quarantine and to be multiplied and evaluated; 32 still in quarantine.

China (Dr. Chengdao Li) 50 Grown in quarantine and expected to be released soon. France 10 Still in quarantine.

Italy (Prof. Roberto Tuberosa of Bologna, Italy/ Prof. Gary Muehlbauer of Minnesota, USA) 220

One of the two populations was developed for rain-fed agriculture (double haploid of BC1F2 from a cross between cultivar Barke and accession HOR11508). The second population was BC2F7 of a cross between cultivar Harrington and accession OUH602 bred for multiple disease resistance. Still to be quarantined.

Japan (Dr. Sato Kaiziro/ Ms Amanda Box) 247

Are mainly of hull-less types. Only 18% of 122 accessions grown to be quarantined have so far produced seeds.

South Africa (Francois Potgieter) 44

South Africa Barley Breeding Research Institutes advanced lines. Released from quarantine and to be multiplied and evaluated.

Syria (ICARDA/ Dr Sanjiv Gupta) 130 Of diverse origin still to be released from quarantine. Uruguay (Sylvia German) 30 Still in quarantine

Local introduction (Table 2) is also one of the mechanisms of acquiring germplasm to the UWA barley germplasm collection for long term conservation and various research purposes. This includes AWCC accessions, which have basically germplasm of diverse international origins, elite breeder’s disease nursery and stage 3 of DAFWA breeding lines. Unlike introductions from overseas, these lines are directly included in the evaluation component of the program. Table 2. Local importations for 2008-09

Institution (Donor) No. Notes AWCC (G. Grime) 744 Diverse origin of which 250 are to be evaluated in 2009. QDPI&F (G. Platz) 119 EBDSN in 2008. DAFWA (C. Burchel) 53 Stage 3 entries of DAFWA in 2008

3. Seed bulking Germplasm from various origins (Table 3) were introduced during the previous phase of the UWA-GRDC barley germplasm program. In order to evaluate the response of these accessions to biotic and abiotic factors, the seed of the introduced materials were bulked after released from quarantine. In the seed multiplication nursery, data like seedling growth habit, resistance to voluntarily established diseases, days to awn appearance, plant height at maturity, row number and any other agronomic traits of the accessions were recorded. The seed (10 – 30 g) of more than 100 accessions and data associated with them were sent to the three nodes of BBA for further study. Accessions with special traits as characterized by their region of origins, where certain stress factors are prevalent, were sent to programs researching on these.


Table 3. Germplasm bulked and dispatched during 2008/09 Source region

No. Seedling growth habit

Row Number

Plant Height (cm)

Days to anthesis

Traits

America 48 Erect to very prostate

2/ 6 45 – 100 82 – 148 Malting, feed, drought and acid soil tolerance, Russian wheat aphid resistance

Europe 40 Erect to very prostate

2/ 6 45 – 100 82 – 144 Malting, feed, drought and acid soil tolerance

North Africa 6 Erect to semi-erect

2/ 6 75 – 105 86 – 111 Drought and acid soil tolerance

South Africa 14 Erect to semi-prostate

2 55 – 80 83 – 97 Malting and feed

4. Evaluation 4.1 Screening for disease resistance Scald (Rhynchosporium secalis), spot form net blotch (Dreschlera teres f. maculata), powdery mildew (Erysiphe graminis) and barley leaf rust (Puccinia hordei) are some of the major cereal diseases posing potential treat to barley production in Australia. Developing resistant varieties is the best way of controlling these diseases. Thus, assessing various germplasm for disease resistance and making them available for different breeding and research programs is the core point for developing disease resistant cultivars. Separate nurseries were established to screen different germplasm for scald, spot form net blotch (SFNB) and powdery mildew under field conditions at Shenton Park. Germplasm screened for scald and SFNB included old UWA collection material, field introductions of 2007, elite breeder’s disease nursery (EBDSN) of 2008 and known controls (Table 4). Accessions were planted in hill plots at intra-raw spacing of 25 cm. Straw from the previous season disease nurseries was used as inoculum for the establishment of scald and SFNB diseases in respective nurseries. The nursery for powdery mildew was left for natural infection. Table 4. Germplasm screened for resistance to scald and spot form of net blotch.

Germplasm No entries Comment Old UWA germplasm accessions (WA02001 to WA02500) 500 69 lines showed resistance Introductions including both from overseas and local (2007) 385 27 lines showed resistance EBDSN (2008)-replicated twice 119 Data provided to the national coordinator Controls and others 32 Planted in 220 hill plots across the nursery

4.1.1 Scald Disease infection across the nursery was very high. Accessions were scored for their resistance to scald at different growth stages: 65, 91 and 115 days after planting. The establishment of spot form net blotch (SFNB) was relatively high across the scald nursery for which the accessions were also rated at 77, 86 and 116 days after planting. Though the infection was not uniform across the nursery, data for powdery mildew was also taken at 120 days after planting. Response to scald and SFNB varied from stage to stage. This was reflected by lack of strong correlation between resistance levels at different stages (Table 5). Although the correlation between accessions response to scald at early and later stages was low, some of them exhibited sustained resistance to both diseases throughout their growth period. Entries of these types and


others that have shown only moderate susceptibility only once at the three stages of SFNB scoring have been sent to various pathology groups for further test (Appendix 1). Among the group of germplasm tested, the majority of resistant lines were identified from old UWA collection material and from recent introductions from overseas (Table 4). Table 5. Correlation coefficient between entries’ response to scald (SC), spot form of net blotch

(SFNB), powdery mildew (PM) at different growth stages (number in parenthesis indicating days after planting to disease scoring).

Traits SC1 (65)

SC2 (91)

SC3 (115)

SFNB1 (77)

SFNB2 (86)

SFNB3 (116)

PM (120)

Days to anthesis

SC1 (65) *** 0.58 0.51 -0.08 -0.29 -0.38 -0.04 -0.20

SC2 (91) *** 0.76 -0.15 -0.44 -0.53 -0.06 -0.24

SC3 (115) *** -0.16 -0.31 -0.44 -0.09 -0.45

SFNB1 (77) *** 0.41 0.46 -0.01 0.11

SFNB2 (86) *** 0.65 -0.02 0.04

SFNB3 (116) *** -0.04 0.18

PM (120) *** -0.01

Days to anthesis *** 4.1.2 Spot form net blotch The similarity in accession resistance to SFNB at various growth stages varied considerably (Table 6). The majority of the accessions maintained their resistance level at 105 and 151 days after planting (r = 0.73), while the opposite was observed when the early resistance was compared with both the later two. A considerable number of accessions, mainly old UWA collection material, were identified as resistant types (Appendix 2) and have been sent to various pathology groups for further evaluation. Table 6. Correlation coefficient between accessions response to spot form of net blotch at

various growth stages and scald (SC) (number in parenthesis indicates days after planting to disease scoring).

Traits SFNB1 (65) SFNB2 (105) SFNB3 (151) SC (106)

SFNB1 (65) *** 0.39 0.37 -0.07 SFNB2 (105) *** 0.73 -0.26 SFNB3 (151) *** -0.31 SC (106) ***

4.1.3 Powdery mildew Old UWA germplasm accessions WA02001 to WA2500, controls were tested for their resistance to powdery mildew under field conditions. All, except one, of the accessions from old UWA collections were identified to be resistant types (Appendix 3). The seedlings of more than 700 accessions were raised twice in the glasshouse to screen resistant types at seedlings stage. Unfortunately, both trials failed due to the lack of disease establishment this year.


4.1.4 Collaborative works 4.1.4.1 Barley Leaf Rust (Puccinia hordei) Screening of barley accessions against barley leaf rust (BLR) was conducted in collaboration with Mr. Jason Bradley of DAFWA. The seedlings of more than 670 accessions were raised (16/10/2008) at Shenton Park Field Station of UWA till they reach 2 leaves growth stage and transferred to DAFWA, South Perth. The seedlings were inoculated with a mixture of 5453P+ and 5453P- isolates under controlled relative humidity and temperature. The accessions were scored for their resistance to BLR on 17/11/2008 using 0 – 4 scale, where 0 was a complete resistance/ no infection and 4 being very susceptible. One hundred and thirty (130) of the tested accessions exhibited resistance ranging from very strong to moderate (Appendices 4 and 5). The rest of the accession showed various degree of susceptibility (data not shown). In both resistant and susceptible groups, some of the accessions showed different level of resistance within the same accession indicating there might be segregation for resistance or seed mixture. 4.1.4.2 Other diseases Accessions that have been identified as resistant to scald and spot form net blotch during 2008 season (Appendix 1; 2) were sent to our collaborators at the Department of Food and Agriculture of Western Australia (DAFWA), Department of Primary Industries and Fisheries of Queensland (QDPI&F), Victoria (VIC-DPI) and the Southern Australia Research and Development Institute (SARDI) for advanced evaluation. Considerable number of accessions were introduced during the previous phase of this program., among which 239 were tested for their resistance to isolate SNB320 of spot form net blotch (SFNB) and isolate NNB330 (Skiff virulent) of net form net blotch (NFNB) under field condition at QDPI&F; to BLR and powdery mildew at DAFWA. These accessions were among several materials included in UWA’s SFNB and scald disease nurseries in 2009. Unlike the test conducted at QDPI&F where a single isolate was used, the straw from the previous season’s disease nurseries was used to establish disease at Shenton Park. This indicates the sever pressure the accessions endure were exposed to under the later condition. The percentage of plants showing BLR symptoms under field conditions was used to identify resistant accessions at adult stage ,where accessions with < 40% infection were considered as resistant types (Appendix 6). A scale of 0 (very resistant) to 9 (very susceptible) was used for NFNB, SFNB and powdery mildew; whereas a scale of 0 (very resistant) to 5 (very susceptible) was used for SFNB and scald at UWA. Accessions with a scale of 4 or less were considered as resistant type for NFNB and those with 5 or less for SFNB. Out of the 239 accessions tested, several of them were identified to be resistant to SFNB (43) and NFNB (180). Forty accessions were resistant to both isolates of NFNB and SFNB. (Appendix 6) Sixteen accessions were tested for their resistance to four isolates of scald at SARDI. Accessions Quina (WA5125/ WA5919); CI9819 (WA6553); Hml-02/Arabiabiad, AUS408933, 52ZBB0 (WA8834); Pamunkey, PI583865 (WA10353); BVDV-030 (WA11091) exhibited promising resistance level ranging from strong resistance to moderate resistance to all 4 isolates.


4.2 Response to photoperiod and basic vegetative period Response to photoperiod is the difference between number of days to awn appearance under natural and extended light condition. Basic vegetative period is the number of days to awn appearance under extended light condition. Two nurseries with 750 accessions were set up on June 12th 2008 to estimate their response to the change in photoperiod and basic vegetative period requirement. One of the nurseries was established under natural light condition, while the second one was under 18 hours light supply. Germplasm included in this study were imported accessions from overseas, lines at various stages in the breeding program stages of BBA and controls. The result (Fig. 1 and 2) from this study demonstrated that there is a strong variation among entries in their response to photoperiod and basic vegetative period requirement. This is one of the most important characteristics which should be assessed for all accessions particularly new introductions.

0

5

10

15

20

25

30

35

-30 -20 -10 0 10 20 30 40 50

Response to photoperiod (days)

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Fig. 1. Response to photoperiod of 750 barley accessions

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45 55 65 75 85 95 105 115 125

Basic Vegetative Period (days)

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Fig. 2. Basic vegetative period (BVP) of 750 barley accessions


4.3 Boron toxicity Boron toxicity is one of the abiotic stress factors reducing the growth and development in barley causing substantial yield loss. Barley plants grown in conditions with high boron concentration show the toxicity symptoms from the tip progressing towards the bottom of their first leaf. The symptoms show on the second, third, fourth leaves etc sequentially in the same manner. The damage due to toxicity is more pronounced during hot, dry summer compared to cool and relatively humid winter (Rodger Boyd, 2009; personal communication). The high evapo-transpiration forces the plants to take up and accumulate relatively high boron levels exacerbating damage during summer. To establish their profile in response to boron toxicity and identify tolerant types, various accessions were screened under high boron concentration supplied in semi-hydroponic system to barley seedlings grown in sand culture. The trial was established on 12-13th March 2008 with water only using 307 accessions of which 7 failed to produce viable plants. The screened accessions included introductions in 2007, EBDSN materials from 2008, wild types, tolerant lines identified in 2007 and controls. Nine days after planting solutions of macro- and micro-nutrients were added to the tank supplying water to the plants grown on the sand culture. Twenty (20) ppm of boron solution was added to the tank 15 days after planting. Accessions were scored for their response to boron toxicity 6-7 days after the application of boron, which was at 3 leaves development stage. Accessions previously known as very tolerant, moderately tolerant and sensitive types were used as differential controls. Five accessions from each tolerant group were planted in 5-7 replications. Some of the controls exhibited different response than expected during this seasons test. To develop a classification scale (Table 7) and categorize barley accessions on the basis leaf area damage (LAD) from boron toxicity, accessions CM72 (WA01750), Sahara 3769 (WA07099) and Onslow (WA07102) were used as reference. Table 7. Criteria to classify barley accessions in to different tolerance categories for damage by

boron toxicity at seedling stage, where LAD is Leaf Area Damage. Estimated LAD (%)

Tolerance category Comment

0-5 Very tolerant

Up to 30% of 1st, 20%th of 2nd and 10% of 3rd leaves covered with toxicity symptom, with overall estimated LAD on all leaves less than 5%. Accessions with high damage to either 1st or 2nd leaf area were considered to have low level of tolerance even if estimated LAD was <5%.

6-10 Tolerant

The 1st, 2nd and 3rd leaves, respectively, showing 50%, 40% and 30% symptoms at the upper tips. About 10% of LAD is estimated for all leaves. However, accessions with symptoms of greater than 60% and 20% on 1st or 2nd leaf, were deemed to have lower tolerance potential even if the estimated LAD was <10%.

11-20 Moderately tolerant

Maximum of 60% of 1st and 2nd leaves and 20% the 3rd leaf covered by toxicity symptom. Accessions with the worst damage to all the first three leaves were considered to have lower tolerance level even if the estimated LAD was < 20.

21- 30 Moderately sensitive Up to 100%, 90%, 40% of 1st, 2nd and 3rd leaves, respectively, covered by toxicity symptom.

31-50 Sensitive Up to 100%, 90%, 40% of 1st, 2nd and 3rd leaves, respectively, covered by toxicity symptom.

>51 Very sensitive Up to 100% of both 1st and 2nd, and 60% of 3rd leaf of accessions covered toxicity symptoms.

Accession CM72 has previously been identified as tolerant type. However, significant damage to its first and second leaves; minor symptom on the third leaf at the time of scoring was observed. It showed 5-30% LAD with 15% average across 7 replications. This demonstrated a moderate tolerance level for CM72 previously thought to have complete tolerance. Accession Sahara 3769 showed no symptom in all 5 replicates of this season’s screening nursery indicating its very high tolerance character. On the other extreme, accession Onslow was found to be very sensitive, where its first and second leaves showed complete damage. The third leaves in


all 5 replications were damaged considerably. It exhibited 50-80% LAD with 68% average indicating the high level of sensitiveness of this accession. As in the controls, accessions varied considerably (Fig. 3) in their response to boron toxicity. The number of accessions identified to very tolerant, tolerant, moderately tolerant were 32, 27 and 32, respectively (Appendix 7). A further experiment established using 20 accessions of diverse origin grown over a longer period has shown that boron toxicity has detrimental effect on the dry matter accumulation at an early stage of the plant. Moreover, the damage to the plants continued with the new emerging leaves beyond the third leaf stage. This experiment is continuing to observe the effect of boron on other agronomic traits.

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Fig. 3. Contrasting response to boron toxicity in barley. 4.4 Previously enhanced lines Extensive work has been undertaken during the previous phase of UWA/GRDC barley germplasm project with a genetic enhancement component. This part of the program has provided an unprecedented support to the overall barley industry of Australia. Thirty five populations (Appendix 8) were developed for various objectives including one or more combinations of plant height/dwarfism, adaptation, grain yield, resistance to various diseases, malting quality, barley leaf rust resistance, polyphenol oxidase content and globose seed shape (Grime, 2006). The majority of the crosses used current commercial cultivars as one parent with the second parent carrying the trait of interest. A nursery, in which each entry was grown in a single hill plot at a spacing of 25 cm, was established from F3 seeds of these populations to identify promising ones for the character of interest. Field observations of basic agronomic and seed physical traits, resistance to powdery mildew or spot form net blotch were used to determine lines to be advanced in any of BBA evaluation programs. Seeds of the selected lines were harvested separately and weighed. In addition to the seed weight, other agronomic and disease resistance data of the harvested lines were further considered for critical selection. Depending on the quantity of the seed harvested, selected lines of 18 populations were sent to one or more nodes of BBA. They will be field grown and compared with other lines developed by respective node’s breeding programs. F4 seeds of seven populations bred for disease resistance will be evaluated in 2009 at Shenton Park Field Station.


5. Appendixes Appendix 1. Scald (Rhynchosporium secalis) resistant accessions.

WA No Name / Pedigree WA No Name/ Pedigree 00001 Bald Skinless 02300 GALLEON 02046 02324 ARMELLE 02047 02325 ARVELLE 02048 02329 ATLAS 46 02054 TOKAK 02330 ATLAS 57 02060 TREBI 02331 ATLAS 68 02061 TURK 02332 ATLAS 74 02063 EGYPT 02334 BETZES RH 02066 OSIRIS 02335 BRIER 02071 WW*Glabron 02362 CI6225 02073 CAL.1311 02365 CI7584 02082 WU 3071 02367 CI8618 02119 102-10-21 02382 CI9764 02123 T111-2-1 02394 CI14039 02124 T111-2-2 02398 CI14132 02125 T111-2-3 02442 PIONEER ENGLAND 02127 T111-2-5 02456 SADAF 217/51 02129 T111-2-7 02457 SADAF 233/51 02130 T111-2-8 02481 CPI 9779 02132 T111-2-10 02490 PERBETERI IH-3 02133 T111-2-11 02551 Sultan 02134 T111-2-12 10461 H90022001005 02135 T111-2-13 11088 BVDV-027 02136 T111-2-14 11292 C04A-37 02145 T111-3-4 11293 C04A-38 02146 T111-3-5 11496 UWA scald seln99T005 02149 T111-3-8 11508 Rawson 02150 T111-3-9 11521 CI 4023 02151 T111-3-10 11524 CLE 232 02153 T111-3-12 11548 WABAR2471 02154 T111-3-13 11549 WABAR2473 02157 T111-3-16 11551 WABAR2478 02167 WB193-52-1 11582 NRB07136 02168 HOMER 11583 NRB07141 02169 HALCYON 11584 W02%915 02170 KASKADE 11587 XB2205-12 02172 HANNIBAL 11623 T31126 02173 KORU 11634 WI4262 02174 WB312-81-3 11635 WI4269 02194 IGRI * PGG12208 (79.36)/7 11645 WI3930 02200 IGRI * PGG12208/8 11647 Yangsimai 3 02201 IGRI * PGG12208/4 11662 VB0706 02204 IGRI * PGG12208 (79.13)/41 11671 TR06108 02206 IGRI * PGG12208 (13*7)/3 11676 HB390 02209 IGRI * PGG12208 (13*7)/1 11680 HB394 02210 IGRI * PGG12208/1 (F4) 11684 SB03006 02217 (79.13) /18 11718 BM9935-70 02265 C134-APMxCI0871/complex 9th IBDN 11720 BM9936-80


Appendix 2. Spot form net blotch (Dreschlera teres f. maculata) resistant accessions.

WA No Name / Pedigree WA No Name / Pedigree 02001 H.SPONT. X-2 02300 GALLEON 02022 H.SPONT. X-25 02311 WI-2721 02023 H.SPONT. X-26) 02324 ARMELLE 02025 H.SPONT. X-28 02327 ATEM 02026 H.SPONT. X-29 02331 ATLAS 68 02028 H.SPONT. X-31 02362 CI6225 02030 H.SPONT. X-33 02371 CI9214 02031 H.SPONT. X-34 02372 CI9440 02037 H.SPONT. X-40 02376 CI9696 02039 H.SPONT. X-43 02398 CI14132 02051 SECOBRA 4681 02402 CI16140 02053 TRON 02443 PIONEER WINTER 02054 TOKAK 02481 CPI 9779 02055 GRIMMET 02486 NO-071 02059 FANETT 02487 CHEVALIER W45A 02060 TREBI 02488 AGRICRITHON 02063 EGYPT 02490 PERBETERI IH-3 02065 ODENBRUCKER 02498 NAKING 450 02068 STEUDELLI 11145 TR05386 02071 WW*Glabron 11268 H94084001 02075 84001 11323 Tenn. Awnless D22-5 02078 LADA 11493 CAPSTAN 02087 74S/313 11499 FLAGSHIP 02092 WI-2691 11502 MARITIME 02093 WI-2692 11511 DICTATOR 02102 KLAGES 11629 WI3416-1572 02103 DAMPIER/KLAGES 1 11634 WI4262 02104 DAMPIER/KLAGES 12 11640 WI4062 02105 DAMPIER/KLAGES 13 11641 WI4075 02115 DAMPIER/KLAGES 79 11642 WI4083 02119 102-10-21 11643 WI4230 02123 T111-2-1 11644 WI4215 02124 T111-2-2 11646 WI3693 02125 T111-2-3 11647 Yangsimai 3 02127 T111-2-5 11655 VB0432 02129 T111-2-7 11659 VB0612 02130 T111-2-8 11660 VB0613 02132 T111-2-10 11672 TR06109 02134 T111-2-12 11673 TR06389 02135 T111-2-13 11685 SB03015 02232 1004/11 PLOT443 11687 SB040197 02233 (2 * 7)/1 (F4) PLOT001 11688 SB040953 02251 B.VOLLA 11689 SB040369 02252 C208 11691 SM04351 02265 C134-APMxCI0871/complex 9th IBDN 11694 SB040216 02266 C134-APMxMIDUCA/complex 9th IBDN 11713 BM9917-54 02280 EH8B KENYA CBR 141 11762 2B03-3903 02291 CAL.MARIOUT 11783 Z125O126O


Appendix 3. Powdery mildew (Erysiphe graminis) resistant accessions.

WA No Name / Pedigree 00001 Bald Skinless 00976 81-82/010 02001 H.SPONT. X-2 02003 H.SPONT. X-5 02005 H.SPONT. X-7 02006 H.SPONT. X-8 02008 H.SPONT. X-10 02009 H.SPONT. X-11 02013 H.SPONT. X-16 02015 H.SPONT. X-18 02017 H.SPONT. X-20 (CLIPPER m) 02018 H.SPONT. X-20 02020 H.SPONT. X-23 02021 H.SPONT. X-24 02022 H.SPONT. X-25 02023 H.SPONT. X-26) 02025 H.SPONT. X-28 02026 H.SPONT. X-29 02032 H.SPONT. X-35 02033 H.SPONT. X-36 02040 H.SPONT. X-44 02051 SECOBRA 4681 02058 KAYA 02062 CEPADA CAPA 02093 WI-2692 02119 102-10-21 02121 110-13-22 02171 BR 302,02 02173, 02181 KORU 02175 WPBS 320/81 02179 CSB 449-8 02180 KM123 02191 IGRI * PGG12208/7 02266 C134-APMxMIDUCA/complex 9th IBDN 02279 NIGRINUDUM 02281 PITAYO-RM1508/complex BYDV-35 02326 ASSE 02332 ATLAS 74 02342 CI1179 02354 CI4797 02392 CI9819 02399 CI16137


Appendix 4. Barley accessions with strong resistance to barley leaf rust (Puccinia hordei) at seedling stage.

WA No Name / Pedigree WA No Name / Pedigree 10124 KOMPAKT 10878 CBSS98Y00855T-F-0Y-0M-2Y-1M-0Y

10521 Auriga 10879 CBSS99Y00038S-6Y-2M-0Y

10523 Xanadu 10882 CBSS98WM00110T-0TOPY-0M-1Y-1M-0Y

10750 WI-3930 10883 CBSS97M00848T-G-1M-1Y-1M-3Y-1M-0Y

10848 CBSS98Y00759T-E-0Y-0M-1Y-1M-0Y 10888 CBSS98WM00002S-0M-8Y-2M-0Y

10876 CBSS98Y00563T-A-0Y-0M-1Y-1M-0Y 10892 CBSS98Y00542T-C-0Y-0M-1Y-1M-0Y

10890 CBSW97WM00064T-C-1M-1Y-1M-1Y-1M-0Y 10894 CBSS98WM00059T-0TOPY-0M-8Y-2M-0Y

3699 AUSTRALIAN NO.22 10900 CBSS98WM00056T-0TOPY-0M-1Y-1M-0Y

4654 Malvaz 10901 CBSS97M00836T-A-1M-2Y-2M-1Y-1M-0Y

6110 TR328 10906 CBSS97WM00227T-C-1M-3Y-1M-1Y-1M-0Y

6799 SHYRI 10917 CBSS97M00850T-U-2M-2Y-2M-1Y-1M-0Y

9261 CLE 231 10922 CBSS98Y00311T-A-0Y-0M-1Y-1M-0Y

9773 HOR 11225 10927 CBSS98Y00295T-D-0Y-0M-2Y-1M-0Y

10464 Z96408001 10929 CBSS98Y00495T-B-0Y-0M-2Y-1M-0Y

10485 Hydrogen 10931 CBSS99Y00470T-0TOPM-4Y-1M-0Y

10507 Olbram 10935 CBSS98Y00607T-C-0Y-0M-1Y-2M-0Y

10515 Ursa 10938 CBSS98Y00638T-B-0Y-0M-1Y-1M-0Y

10520 Harriot 10939 CBSS99Y00050S-7Y-1M-0Y

10523 Xanadu 10943 CBSS98WM00260T-0TOPY-0M-3Y-1M-0Y

10668 BOWMAN/Rph3.c 10946 CBSS98Y00804T-A-0Y-0M-1Y-2M-0Y

10672 BOWMAN/Rph5.e 10947 CBSS98Y00803T-A-0Y-0M-1Y-1M-0Y

10714 WABAR2315 10949 CBSS99Y00032S-8Y-1M-0Y

10738 T98189 10953 CBSS98Y00818T-L-0Y-0M-1Y-1M-0Y

10742 WI-3804 10959 CBSS97WM00130T-D-2M-1Y-1M-1Y-2M-0Y

10752 Orox 10964 CBSS98WM00176T-0TOPY-0M-2Y-2M-0Y

10756 VB0204 10974 CBSS97M00877T-E-3M-2Y-1M-4Y-0B-0Y

10764 VB0419 10979 CBSS98WM00229T-0TOPY-0M-1Y-2M-0Y

10766 VB0424 10983 CBSS98Y00627T-H-0Y-0M-1Y-1M-0Y

10767 VB0428 10988 CBSS979M00600T-H-1M-2Y-2M-1Y-1M-0Y

10798 CBSS98Y00546T-F-0Y-0M-1Y-1M-0Y 10989 CBSS98WM0027S-0M-2Y-2M-0Y

10819 CBSS98Y00819T-G-0Y-0M-1Y-1M-0Y 10993 CBSS98WM00001T-K-0Y-0M-1Y-2M-0Y

10823 CBSS98M00001S-0M-3Y-1M-0Y 11018 CBSS98M00076T-0TOPY-0M-1Y-1M-0Y

10831 CBSS99Y00322T-0TOPM-1Y-2M-0Y 11020 CBSS98Y00755T-U-0Y-0M-1Y-2M-0Y

10835 CBSS99Y00003S-2Y-1M-0Y 11030 CBSS98Y733T-C-0Y-0M-2Y-2M-0Y

10836 CBSS99Y00096S-2Y-1M-0Y 11037 CBSS99Y00348T-0TOPM-11Y-1M-0Y

10837 CBSS99Y00483T-0TOPM-3Y-2M-0Y 11038 CBSS99Y00377T-0TOPM-3Y-1M-0Y

10838 CBSS99Y00412T-0TOPM-2Y-1M-0Y 11048 CBSS98WM00001T-P-0Y-0M-2Y-1M-0Y

10841 CBSS99Y00368T-0TOPM-1Y-2M-0Y 11049 CBSS99Y00043S-21Y-1M-0Y

10846 CBSS98WM00196T-0TOPY-0M-2Y-0Y 11053 CBSS98M00022T-0TOPY-0M-1Y-2M-0Y

10847 CBSS98Y00586T-A-0Y-0M-1Y-1M-0Y 11055 CBSS98Y00127S-0Y-0M-2Y-2M-0Y

10849 CBSS98Y00855T-F-0Y-0M-2Y-2M-0Y 11070 BVDV-009

10856 CBSS97Y00844T-B-0Y-0M-2Y-1M-0Y 11078 BVDV-017

10858 CBSS99Y00440T-0TOPM-3Y-1M-0Y 11099 UVB 29

10861 CBSS99Y00469T-0TOPM-5Y-1M-0Y 11100 UVC 8

10862 CBSS98Y00819T-G-0Y-0M-1Y-2M-0Y 11129 SR411

10877 CBSS99Y0036S-7Y-2M-0Y


Appendix 5. Barley accessions with moderate resistance to barley leaf rust (Puccinia hordei) at seedling stage.

WA No Name / Pedigree 5818 CHARIOT

7761 Atem

9369 HB372 (wx)

9605 MACKAY

9609 SLOOP SA

9811 Marocco

9868 AEMELLE

9882 HANNCHEN

9916 WABAR2344

9935 WABAR2352

9999 BULOKE

10117 LIBRA

10250 I92-209

10295 TR04282

10421 GSHO 2479

10474 H93107001

10496 Luxor

10522 Ursa

10570 SN22

10718 WABAR2331

10719 WABAR2332

10732 T20559

10735 T21636

10736 T21676

10741 WI-3586-1747

10771 WB269

10842 CBSS99Y00344T-0TOPM-1Y-1M-0Y

10870 CBSS99Y00469T-0TOPM-1Y-2M-0Y

10903 CBSS99Y00350T-0TOPM-4Y-3M-0Y

10914 CBSW98WM00011S-6Y-1M-0Y

11050 CBSS98Y00767T-E-0Y-0M-1Y-2M-0Y

11108 HB388

11118 SB02763

11128 SR409

11132 SR413

11139 TR05101

11140 TR05104

11146 TR05387

11147 TR05388


Appendix 6. Resistance of 2007 introductions to powdery mildew (PM) and Barley Leaf Rust (BLR) tested at DAFWA; isolates of NNB330 (net form net blotch-NFNB), SNB320 (spot form net blotch-SFNB) tested at QDPI&F; scald and SFNB tested at UWA (AdSt = Adult stage, ErSt=Early Stage, Numbers in the parenthesis indicates days after planting).

WA No

Accession name

BLR (Ad St)

PM (ErSt)

PM (AdSt)

SCALD3 (133)

SFNB3 (151)

SFNB (SNB320)

NFNB (NNB330)

09631 PRIMUS R R R S S

11063 BVDV-002 R R MR MS MS S R

11069 BVDV-008 R R R MR MS S R

11083 BVDV-022 R R MS MS MS S R

11085 BVDV-024 R R MR R MS S R

11086 BVDV-025 R MR S MS MS S R

11088 BVDV-027 R R S MS MS S R

11094 UVA 17 S R R MS MS S R

11095 UVA 21 R R R R MR R R

11096 UVA 22 R S MR MS MS S R

11102 UVC 25 R MR MS MS MS S S

11103 UVC 29 R R R MS MS S R

11104 UVC 35 R R MR MS MS S R

11105 UVC 36 R R MR MS MS S R

11106 HB401 S MR MS MS MS R R

11110 HB386 S MR S MS MS S R

11114 SB02604 MR S MS MS MS R R

11115 SB02135 S R MR MS MS S R

11116 SB03131 MR R R MS MS R R

11117 SB03702 MR S S MS MS R R

11119 SB03072 MR MR S MS MS R R

11120 SM03006 MR MR MS MS MS R R

11121 SM03047 MR MR MS MS MS S R

11122 SM03310 S MR R MS MS S R

11123 SM03415 S R MS MS MS S R

11124 SM03520 MR S S MS MS S R

11125 SM03536 MR S MS MS MS S S

11135 TR05103 S MR MS MS MS S R

11136 TR05199 MR MR MS MS MS S R

11137 TR05102 MR R MR MS MS S R

11141 TR05105 S MR S MS MS S R

11145 TR05386 MR MR MS MS MR R R

11151 AMBEV 488 R MR MR MS MS S R

11153 NCL4-98-3-1a R S MR MS MS S R

11156 NCL9-99-9 R R R MS MS S R

11157 NCL11-99-26 R S MS MS MS S R

11166 NCL50-97-1-1 S MR S MS MS S R

11167 NCL50-97-6-4 S MR S MS MS S S

11168 NCL9782 S R MS MS MS S S

11172 NCLU4-1682-29 S MR MS MS MS S R

11173 NDL98224 MR S MS MS MS S R

11174 NDL98505 S R R MS MS S R

11178 Biosaline-02 S MR S MS MS R R

11180 Biosaline-04 S MR S MS MS S R



11184 Biosaline-08 S R/S R/S MS MS S R


Appendix 6. Continued…

WA No

Accession name

BLR (Ad St)

PM (ErSt)

PM (AdSt)

SCALD3 (133)

SFNB3 (151)

SFNB (SNB320)

NFNB (NNB330)


11188 Biosaline-12 S R S MS MS S R


11190 Biosaline-14 S S S MS MS S R





11199 CONRAD S S MS MS MS S R

11200 2B97-4004 MR S MS MS MS S S

11201 2B97-4299-6 MR S MS MS MS S R

11202 2B97-4299-8 MR S S MS MS S R

11206 2B98-2316 S S S MS S S

11207 2B99-2316-4 S S MS R MS S S

11209 2B99-2763-10 S S S R MS S R

11211 2B99-2771 S S MS MS MS S R

11212 2B99-2771-1 MR S S MS MS S R

11213 2B99-2771-9 S S S MS MS S R

11215 Z014J081J R R R MS MS S S

11216 Z11M001M MR R R MS MS S S

11219 Z127O125O R R MR MS MS S S

11220 Z132O003O R MR MS MS MS S R

11226 Z021Q011R R MR S MS MS S S

11227 Z021Q020R R MR MS MS MS S R

11228 Z021Q057R R MR MR MS MS S R

11229 Z023Q020R R R R MS MS S R

11231 Z026Q093R R MR MS MS MS S S

11232 Z037Q001R R S S MS MS S S

11233 BM9732-491-1 MR S S MS MS R R

11234 BM9816-27 S S S MS MS S R

11235 BM9831-42 MR S MS MS MS R R

11236 BM9831D-290 MR MR MR MS MS R R

11238 BM9836-77 MR R MS MS MS R R

11239 BM9845-6 R R R MS MS S S

11241 BM9856D-30 R S S MS MS S R

11243 BM9856D-151 MR S MS MS MR R R

11245 BM9856D-236 MR S S MS MS R R

11246 BM9857-144-1 R S S MS MR R R

11247 BM9857-263-1 R MR MS MS MS R R

11248 BM9904DT-17-8 MR S S MS MR R R

11249 BM9924D-152 MR R R MS MS S R

11253 H98028023 S S MS R MS S R

11254 H98029005 S R MR MS MS S R

11257 H94003125 MR MR S MS MS R R

11261 H98075009 R S S MS MS R R

11262 H94034003 R R R MS MS S R

11263 H94035132 MR MR MR MS MS S R

11264 H94040008 MR R MS MS MS S R

11266 H94059112 MR S MS MS MS R R

11267 H94061120 MR R R R MS S S

11268 H94084001 MR S MS MS MS S S

11276 CLE241 R S S MS MS S R

11280 CLE245 R R MS MS MS S R



WA No

Accession name

BLR (Ad St)

PM (ErSt)

PM (AdSt)

SCALD3 (133)

SFNB3 (151)

SFNB (SNB320)

NFNB (NNB330)

11288 C04A-33 R R MS MS MS S R

11289 C04A-34 S S S MS MS S R

11290 C04A-35 R MR MS MS MS S S

11291 C04A-36 R MR S MS MS S R

11292 C04A-37 R MR S R MS S R

11293 C04A-38 S MR MS R MS S R

11294 OK87854R S R R R MS S R

11296 Sublette S MR MS MS MS S S

11297 Burton S MR MS MS MS S R

11298 Stellar-ND S S S MS MS R R

11299 Goldeneye MR S S MS MS S R

11300 Aquila S S S MS MS S R

11301 Radiant R MR MS MS MS S R

11304 Severa MR R MR MS S S

11305 Gunnar R R R MR MR S S

11306 Vanja MR R MR MS MS S S

11310 Albaicin S R R MR MS S R

11311 Amuldena MR R MR MS MR S S

11312 Arabella R R R MS MS S S

11313 Irene S R R MS MS S R

11315 TR49 R R R MS MR S S

11316 Zaida MR R R MS MR S R

11317 Troon R R R MS MS S S

11319 Hsin an Tien 1 MR MR MR MS MR S S

11323 Tenn. Awnless D22-5 R R R MS MR R R

11494 KEEL S S S MS R S S

11495 CHARIOT (BLR APR) R R R MS MS R S

11496 UWA scald seln99T005 S R R R MS S R

11497 UWA ss seln96TK019 S R R MS MS S R

11498 BULOKE MR R R MS R S R

11499 FLAGSHIP R S S MS MR S R

11500 MACKAY R R R MS MS S S

11501 GROUT S R R MS MR S R

11502 MARITIME S S S MS MR S R

11503 Bichy 2000 MR MR MR MS MS S R

11505 Canela R R MS MS MS S R/S

11506 F103-61 S S S MS MS S R/S

11508 Rawson R MR S MR MS S R

11509 ZAU 7 S S S MS MS S R

11510 Zhaori 19 S MR S MS MS S R

11511 DICTATOR MR S S MS MR S R

11512 COCKTAIL R R R R MS S S

11513 WICKET R R R MR MS S S

11514 BRAEMER R R R MS MS S S

11515 WAGGON R R R MS MS S S

11516 NFC TIPPLE R R R MS MS S S

11518 CARAFE R R R MS MS S S

11519 DOYEN R S R MS MS S R

11520 STATIC R R R MS MS S S



WA No

Accession name

BLR (Ad St)

PM (ErSt)

PM (AdSt)

SCALD3 (133)

SFNB3 (151)

SFNB (SNB320)

NFNB (NNB330)

11521 CI 4023 S R S MR MS S R

11522 NIOBE S S S R MS S R

11523 CLE 226 R S MS MS MS S R

11524 CLE 232 MR S S R MS S R

11525 CLE 233 R MR MS MS MS S R

11526 CLE 234 R MR S MS MS S R

11527 CLE 235 R S MS MS MS S S

11528 CLE 236 R MR MR MS MS S R

11529 CLE 240 R/S R MR MS MS S R/S

11530 CLE 241 R S MS MS MS S R



11533 CLE 244 R R R MS MS S S

11534 CLE 245 R R MS MR MS S R

11535 CLE 246 R S S MS MS S R

11670 TR06107 S MR MS MS MS S R

11671 TR06108 MR MR MS R MS S R

11672 TR06109 MR MR S MS R R R

11673 TR06389 S S MS MS MR S R

11675 TR06391 S S S MS MR R R

11676 HB390 MR R R R MS S R

11677 HB391 S MR MR/S MS MS S R

11678 HB392* MR MR MS R MR S R

11679 HB393* S MR MR MS MS S S

11680 HB394 MR R R R MS S R

11681 HB395 S R MS MS MS S S

11682 SB03067 MR MR MR MS MR R R

11684 SB03006 S S S R MS S R

11685 SB03015 S S S MS R R R

11686 SB040173 MR R MR MS MS S R

11687 SB040197 S S S MS R R R

11688 SB040953 S R R MS R S R

11689 SB040369 S S MS MS MR S R

11690 SB040947 S R MR MS MR S R

11691 SM04351 MR R MR MS MR S R

11693 SM04568 MR MR MS MS MS S R

11694 SB040216 MR R MR MS R R R

11695 SM05432 MR MR MR MS MR S R

11696 SR415(M) MR S S MS MS R R

11697 SR416(M) S S S MS MS R R

11698 SR417(M) R S S MS MS S R

11699 SR418(M) S S S MS MR R R

11700 SR419(M) S S S MS MS S R

11701 SR420(M) S S S MS MR R R

11702 TR06295 S S S MS MS S R

11703 TR06296 S S MS MS MR R R

11704 TR06297 S S S MS MR R R

11707 BM9929-88 MR MR MS MS MR R R

11708 BM9842-71 S R R MS MR S R

11709 BM9846-32 MR MR MS MS MR R R

11710 BM9915-34 MR S S MS MR R S

11711 BM9915-45 S S S MS MR R R

11712 BM9915-58 MR S S MS MS R R



WA No

Accession name

BLR (Ad St)

PM (ErSt)

PM (AdSt)

SCALD3 (133)

SFNB3 (151)

SFNB (SNB320)

NFNB (NNB330)

11713 BM9917-54 MR S S MS MR R R

11716 BM9921-50 MR S S MS R R S

11717 BM9935-61 S S MS R MS S S

11718 BM9935-70 MR MR S R MS S R

11719 BM9935-76 S S S MR MS S R

11720 BM9936-80 MR MR MS R MS S S

11724 PI 532016 S MR MR S R

11739 PI 531841 S R R MS MS S R

11740 PI 220069 R S S MS MS S R

11741 CONRAD R MR MS MS MS S R

11743 2B00-0342 R MR S MS MS S R

11744 2B01-2010 MR S S MS MS S R

11745 2B01-2015 S S S MS MS S S

11746 2B02-2925 MR MR MS MS MR S R

11747 2B02-2941 S S S MS MR S R

11748 2B03-3560 R S S MS MS S R

11752 2B03-3669 MR S S MS MR S R

11753 2B03-3681 MR S MS MS S R/S

11754 2B03-3719 MR MR MS MS MS S R

11755 2B03-3732 MR MR MR MS MS S R

11756 2B03-3734 S R MR MS MS S R

11761 2B03-3883 R S MS MS MS S R

11762 2B03-3903 MR S S MS MR S R

11763 2B03-3954 R S MS MS MS S R

11764 Z031P134Q R MR MS MS MS S R

11768 Z039P110Q R R R MS MS S S

11769 Z041P121Q MR R R MS MS S S

11770 Z041P179Q R R R MS MS S S

11771 Z042P046Q R MR MS MS MS S R

11772 Z043P022Q R R R MS MR S S

11776 Z053N066N R R R MS MS S S

11778 Z055R006S R R R MS MR S S

11779 Z068R055S MR MR MR MS MS S R

11780 Z090M016M R R R MS MS S S

11781 Z090M066M R R R MS MS S S

11783 Z125O126O R R R MS R S S

11784 B1614 MR S S MS MS S R


Appendix 7. Accessions with tolerance to 20 ppm boron toxicity, where VT is very tolerant, T is tolerant and MT is moderately tolerant.

WA No Name / Pedigree Tolerance WA No Name / Pedigree Tolerance

11973 WABAR2537 VT 11788 UWA tall seln2735 T 11729 PI 354931 VT 11830 Waggon T 11809 UWA 6R seln9619 VT 11933 WI4328 T 11834 Braemar VT 12005 NRB07018 T 11515 WAGGON VT 11730 PI 354943 T 11523 CLE 226 VT 11810 UWA husked seln9761 T 11724 PI 532016 VT 11865 OWB92 T 11727 PI 282610 VT 11885 H95056002 T 11723 PI 406270 VT 11551 WABAR2478 T 11866 OWB-Parent DOM VT 11556 Binalong T 11890 STARS 501B VT 11727 PI 282610 T 11891 STARS 502B VT 11724 PI 532016 T 11892 STARS 503B VT 11887 Chieftan MT

11898 BRAEMAR VT 11893 STARS 504B MT 11905 OLIVEROS LITORAL SAG VT 11894 STARS 507B MT 11911 FLEET VT 11915 VB0609 MT 11923 WI4259 VT 11920 WI4075 MT 11924 WI4262 VT 09794 HOR 13461 MT 11929 WI4301 VT 09837 Spanish Landrace-355 MT 11930 WI4311 VT 11731 PI 355437 MT 11931 WI4323 VT 11790 UWA 2R short seln2737 MT 12012 W02%348 VT 11715 BM9919-90 MT 11178 Biosaline-02 VT 09776 HOR 12517 MT 11180 Biosaline-04 VT 11674 TR06390 MT 11182 Biosaline-06 VT 11799 UWA 6R seln4084 MT 11183 Biosaline-07 VT 11805 UWA 6R hulless seln8635 MT 11187 Biosaline-11 VT 11867 OWB-Parent REC MT 11189 Biosaline-13 VT 12022 W02%434 MT 11191 Biosaline-15 VT 11777 Z055O012O MT 11195 Biosaline-19 VT 11817 WVA 20 MT 11722 PI 405369 VT 09808 Precoce du Maroc MT 09833 Spanish Landrace-338c T 11606 WABAR2420 MT 11876 H95039003 T 09835 Spanish Landrace-349b MT 11903 VOGELSANGER GOLD T 11917 VB0613 MT 11909 BULOKE T 11945 AUS 495145 MT 11913 KEEL T 11804 UWA 6R seln4885 MT 11928 WI4295 T 09819 Rosa MT 11932 WI4326 T 11794 UWA early seln2835 MT 11949 WABAR2312 T 11801 UWA short late seln4153 MT 11956 WABAR2478 T 12021 W02%429 MT 12009 NRB07069 T 11759 2B03-3859 MT 12010 NRB07071 T 11997 NRB05549 MT 09810 Barlis T 09807 Noire Maroc MT & MS

11910 FLAGSHIP T 11793 UWA short seln2794 Moderately tolerant & sensitive

11925 WI4263 T 11926 WI4269 Very tolerant & sensitive

09998 YAMBLA T


Appendix 8 Evaluated population from previous project.

Code Pedigree Parent (female) Parent (male) 06T567 91Wn/Hml 91WINTER Hamelin

06T568 Ant19/Hml Ant19-109 Hamelin

06T591 BnO/Hml Ben Olid Hamelin

06T592 BM9648-14D/Hml BM9648-14D Hamelin

06T599 CLE231/Gdr CLE231 Gairdner

06T601 Dash/SB01178//IBYT02010/Hml Dash/SB01178 IBYT02010/Hamelin

06T612 Gdr/IBYT0210//2357/SB01178 Gairdner/IBYT02010 WABAR2357/SB01178

06T614 Hml/BnO Ben Olid Hamelin

06T619 Hml/I88-549-3 Hamelin I88-549-3

06T620 Hml/I89-420 Hamelin I89-420

06T621 Hml/I93-754 Hamelin I93-574

06T632 Hml//CM72/Nrn/3/Hml//Bln/Kpr Hamelin//CM72/Nairn Hamelin//Blenheim/Kaputar

06T638 I444/2094 I444 WABAR2094

06T639 HOR3877/Hml HOR3877 Hamelin

06T641 I88-549-3/Hml I88-549-3 Hamelin

06T642 IBYT02010/TR328//2344/IBYT0210 IBYT02010/TR328 WABAR2344/IBYT02010

06T659 M931/Hml M931 Hamelin

06T661 MN607/Hml MN607 Hamelin

06T662 ms2096/Dna//Vlm msWABAR2096/Doneria Vlamingh

06T663 ms2096/Dna//Bdn/3/Vlm msWABAR2096/Doneria//Baudin Vlamingh

06T664 N341/2110//2*Vlm N341/WABAR2110//Vlamingh Vlamingh

06T665 N341/2110//Vlm/3/2344 N341/WABAR2110//Vlamingh WABAR2344

06T666 SL44*92C-27/Hml SL44/92C-27 Hamelin

06T667 SN22/Hml SN22 Hamelin

06T668 SVA7/Dash//Sln/Bdn SVA7/Dash Saloon/Baudin

06T671 TR328/2344//2272/Chl TR328/WABAR2344 WABAR2272/Chalice

06T674 WA6370/4/WA898//Ygn/Mny/3/Hml WA6370 WA898//Yagan/Manley/3/Hamelin

06T675 2094/I046 WABAR2094 I046

06T676 2094/I302 WABAR2094 I302

06T677 2094/I346 WABAR2094 I346

06T678 2289/Vlm//2280/Bdn WABAR2289/Vlamingh WABAR2280/Baudin

06T679 2289/WA6370//2240/Sct WABAR2289/WA6370 WABAR2240/Scarlett

06T680 Yan/Bdn YAN 96219 Baudin

06T681 Yan/Hml YAN 96219 Hamelin

06T683 IBYT0210/Vlm//Sln/Vlm IBYT0210/Vlamingh Saloon/Vlamingh

6. Reference: Christy Grime, 2007. Sixth Annual Newsletter of barley improvement through germplasm introduction,

evaluation and enhancement. GRDC Project UWA00096, Varieties, Germplasm Enhancement. University of Western Australia, Australia.

7th annual newsletter of barley improvement through ......park field station. they are evaluated for...

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