20 - agilent lc and lcms portfolio overview

Agilent LC and LCMS Portfolio OverviewAgilent Restricted

November 2007

Agilent LC and LCMS Portfolio Overview

Elaine Ricicki, Ph. D.Life Sciences Marketing Program Manager


November 2007

Customer and Business Trends- across all Industries

Lab situation– Higher throughput, faster turnaround,

lower cost per results– Innovate the processes and methodology– Need to protect investment (methods,

training, infrastructure)

Business trends– International standardization of

methodology – Multi-technique multi-vendor data systems

to reduce maintenance and training effort, facilitate information exchange

– More single-stop shopping for lab instrumentation, software, consumables and services

“ I need to run twice as many samples in half the time with half the resources.”

“Complex samples used to be an occasional challenge. Now they’re a routine complication.”


November 2007

The 1200 Series ConceptI want better, faster….but not different

Reliability

Robustness, Performance

Flexibility

1100 Series

1200 Series

+ Speed+ Resolution+ Sensitivity

+Reproducibility

+ Uptime+ Usability

+ Compliance

1100

HPLC and RRLC


November 2007

The Agilent 1100: The Legacy– Stackable, comprehensive, flexible configurations– Front access for easy user maintenance– Effective leak management– Ergonomic tubing organization for low delay volume and

bandspreading– Single LAN/PC/HUB cable connection– Flexible handheld or PC/network control strategies– Modular intelligence via the CAN network– EMF, Early Maintenance Feedback– Column tracking via RF Tag


November 2007

The New Agilent 1200 Series – over 50 modules!- configure your system without compromises

Better manufacturing quality & improved specs

Max speed & resolution

…plus new controller, software,columns, services and more….Better MS-based solutions & software

NEW Rapid Resolution

LC and LC/MSQuaternary and Binary

Isocratic

Preparative

Capillary and Nano

NEW HPLC-Chip/MS

Max sens., ease of use & robustnessImproved

Usability


November 2007

UltraFast LC (UFLC) – 1st Publication:Standard Agilent 1100 LCZorbax 1.8µm particle Columns• J. Chrom Sci., 38, Dec 2000, pp 535-544

• Author J. J. Kirkland

• Binary 1100 pump

• 1.8 um 4.6 x 30 mm columns

• Applications demonstrating 15 and 24 sec separations

Agilent – Pioneers

in sub-2µm Columns


November 2007

The Van Deemter Plot: Advantages of Smaller Particles at High Linear Velocities

HET

P (c

m/p

late

)

-0.0005

0.0000

0.0005

0.0010

0.0015

0.0020

0.0025

0.0030

0.0035

0.0040

0.0045

5.0 µm

3.5 µm

1.8 µm

2mL/min 5mL/min

Interstitial linear velocity (ue- cm/sec)0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6

Unlike 5µm and 3.5µm columns, 1.8µm columns maintain higher efficiency at higher linear flows, which allows for significant gains in analysis speed. Typical Speed Gain: 5 – 10x


November 2007

ZORBAX Rapid Resolution HT Columns:The Efficiency of Longer Columns

4,200

6000

8,500

12,500

Resolving Power

N(5 µm)

2,5002,10015

6,5004,20030

12,0007,00050

17,00010,50075

24,00014,000100

32,50021,000150

ResolvingPower

N(1.8 µm)

Resolving Power

N(3.5 µm)

ColumnLength (mm)

Efficiency (N)

Pressure

AnalysisTime

PeakVolume

SolventUsage

Rapid Resolution HT columns provide the efficiency of longer columns used for high resolution.

Analysis Time*(Rel. to 150mm)

-33%

-50%

-67%

-80%

-90%

-

Unavailable

Unavailable


November 2007

Actual Example of Particle Size Distribution on ZORBAX 1.8um Particles

Number Average Plot of ZORBAX 1.8 um materialDifferential Number

6543210

500

1000

1500

2000

2500

3000

3500

Particle Diameter (µm)

Num

ber

Number StatisticsMean: 1.740 umMedian: 1.727 umMode: 1.732 um95% Conf. Limits: 1.739 – 1.742 umSD.: 0.271 um

%> 10 90 Ratioum 2.04 1.47 1.39

Small number of “off median” particles visible in number average plot

PSD is “ideal” in terms for largest:smallest particles for high efficiency.Low fines means lowest back pressure.

Low fines

This ratio means good control of PSD


November 2007

ZORBAX RRHT Bonded Phase Choices

ZORBAX Eclipse PlusZORBAX Eclipse Plus – New! (C18 and C8) first choice for method development, industry leading peak shape performance, good for most applications, use up to 60°C. Note: L1 (C18) and L7 (C8) columns – accepted by USP

ZORBAX ZORBAX StableBondStableBond – good for low pH and high temperature applications, for those who want to raise temperature for faster analysis • SB-C18 – use up to 90°C and down to pH <1 (high temp only at low pH) (L1)• SB-C8 – use up to 80°C and down to pH 1 (L7)• SB-Phenyl – use up to 80°C at pH 2 (L11)• SB-CN – use up to 80°C at pH 2• SB-AQ – use in up to 100% aqueous mobile phases for good retention – up to 60°C at

pH 2ZORBAX ExtendZORBAX Extend--C18C18 – good at high pH – up to pH 11.5 at room temperature (L1)

ZORBAX Eclipse XDBZORBAX Eclipse XDB – (C18 (L1) and C8 (L7)) good choice when following method example/application, but older than Eclipse Plus so not the first choice, general purpose bonded phase, for most types of compounds, use up to 60°C

ZORBAX RxZORBAX Rx--SilSil – for normal phase applications and “HILIC” type applications where an aqueous containing mobile phase is used on bare silica.


November 2007

Speed: 1200 Series Rapid Resolution System- the fastest LC in the world

2.1mm x 50mm 1.8µm1.00 ml/min, 40°CAnalysis Time = 1.1min

RRLC, 40°C10x fasterPW = 0.5 sec

min0.2 0.4 0.6 0.8 10

HPLC, 40°CPW = 3.4sec

4.6 x 150mm, 5µm1.20 ml/min, 40°CAnalysis Time = 11min

min0 2 4 6 8 10

• Example: Phenones Test Sample• System: 1200 RRLC System • Columns: Zorbax SB-C18

2.1mm x 50mm 1.8µm2.40 ml/min, 95°CAnalysis Time = 0.4min

RRLC, 95°C

27x faster

PW = 197msec0.20 0.4 min

Liquid chromatographyin millisecond range


November 2007

min0 0.2 0.4 0.6 0.8 1

mAU

0

100

200

300

400

500

600

700

0.209

0.370

0.505

0.620

0.664 0.7

25

0.821

0.908

0.986

1200 Series Rapid Resolution System - efficient use of pressure

A BEH,

2.1 x 50mm,1.7um

RRHT, SB-C18,

2.1 x 50mm, 1.8um

Column

0.914 min0.949 minAnal. Time

700 bar (+60%)440 barPressure

0.18 – 0.30 %RSD0.025 – 0.094 %RSDPrecision

3.503.69Resolution

Suppler B1200 Rapid Resolution

Sample: Phenones Test MixFlow Rate: 1 ml/minGradient: 35-95% ACN in 0.9 minTemperature: 50°Injection volume: 1 µlInjection Technique: ADVR, OI,

MCOWL: 245 nmData Rate: 80 Hz

Better performance at lower pressure than other U-HPLC designs


November 2007

Pharmaceutical Process Control – Converted to High-Throughput LC- customer provided data

min0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8

Norm.

0

20

40

60

80

Norm.

0

20

40

60

80

Optimized process control method System: 1100 Binary SystemColumn: 4.6 x 50 mm, 1.8um RRHTGradient analysis time: 2 minSpeed: 23x faster than 5umTime saving: 95%Feedback Time: < 1h

4σ Peakwidth = 1.5 – 2.5 sec

Rs(3,4) = 1.5

min0 5 10 15 20 25 30 35 40 45

Original process control method System: 1100 Binary SystemColumn: 4.6 x 250 mm, 5um Gradient analysis time: 45 minFeedback Time: > 11 hours

Rs(3,4) = 3.3

4σ Peakwidth = 9 – 18sec

2.0

45

Process monitoring times can be reduced 5 – 25X


November 2007


November 2007

µ-Degasser• Maximum baseline stability and minimum noise by continues

vacuum sourceBinary Pump SL• Configurable delay volume (120µl, 320 µland 600-800µl)

– Standard delay volume (600 – 800 µl) for ultra fast and conventionaLC with 3 and 4.6 ID columns

– Low delay volume (120 µl) for ultra fast and and conventional LC with 2.1 mm ID columns (LC/MS)

– Medium delay volume (320 µl) for ultra fast and superior UV sensitivity with 2.1 mm ID columns

1200 RRLC System Benefits of Key Modules Features

• 0.05 – 5 ml/min flow rate range for highest speed on narrowand standard bore RRHT columns (full pressure range)

• 600 bar pressure for higher speed and resolution in RRLC

• Highest RT precision and lowest mixing noise by new pistoncontrol algorithms (EDC – Electronic Damping Control)*


November 2007

Binary Pump SL - Configurable Delay Volume

Pressure-Sensor

Purge Valve

Mixing-T

Damper

400µl MixerBA

600bar Damper

400µl Mixer

Pressure Sensor

PurgeValve

Std delay volume600-800 µl

for ultra fast with3 and 4.6 mm ID

columns and conventional LC

BA

Pressure Sensor

PurgeValve

600bar Damper

400µl Mixer

Pressure-Sensor

Purge Valve

Mixing-T

Flow Path

Low delay volume120 µl

for ultra fast LC with 2.1 mm ID columns


November 2007

High-Performance ALS SL• Cycle Times 25-30 sec including external needle wash

- with overlapped injections < 1 sec

• Simultaneous speed and carry over optimization by flow through design

– for lowest carry over Injector Purge Kit for viscous and sticky samples

• 0.1 - 100µl (1500µl) injection volume for large linear range without loop changes (< 0.25% RSD from 5 – 100 µL)

• No sample losses

• 2 wellplates or 108 vials extendable with samplecapacity extension (16 – 80 shallow plates)

*patents filed



November 2007

1200 Series h-ALS for High-Speed InjectionsDelay Volume Reduction and Overlapped Injections

Main Pass: Injecting Bypass: Sample Loading

Simultaneous Speed and CO optimization by• Simple „one-valve“ flow-though design• Automatic delay volume reduction (6µl)• Overlapped injection• Programmable sample flush-out time• External needle wash• MCO by additional valve switches


November 2007

Thermostatted Column Compartment SL

• 100°C for higher speed and resolution in RRLC

• Improved Peltier temperature control (< +/- 0.05°C) forhighly reproducible RT

• New low volume heat exchanger for minimized peak broadening on 2.1mm ID columns.

• 600 bar 2Ps/10Pt micro valve for minimum cycle times by ACR (alternating column regeneration, which saves wash & regeneration time)

• PCC (post-column cooling) for minimized UV noise under most demanding conditions (< 50µAU at high flow rates and temperatures)

• For columns up to 30 cm column length*patents filed



November 2007

1200 Series TCC SL Low volume Pre-column Heater and Post-column Cooler

• L-shaped pre-column heater • Volume: 1.6 µl• Mounted on carrier

• U-shaped Post-column cooler • Volume: 1.5 ul• Mounted on Carrier

Choice of Valves2PS/6PT or2PS/10PT


November 2007

The New 1200 Column Compartment SLPost-column cooling

0.685 - Anthracene

min0.5 1 1.5 2 2.5

mAU

-0.05

0

0.05

0.1

0.15

0.2

0.25

• Column Temperature: 80°C • Detector-in Temperature: 50 °C• Noise = 90 uAU• S/N = 3.3

• Column Temperature: 80°C • Detector-in Temperature: 24 °C• Noise = 30 uAU• S/N = 10• No Peak Broadening

Column: 4.6 x 50mm, 1.8um

Flow Rate: 4ml/min

Noise reduction at high flow ratesand high temperatures


November 2007

The Agilent 1200 Series 80Hz DAD SL and MWD SLThe 1st Diode-Array Detector designed for Ultra-Fast LC

Tungsten lamp Long-life

Deuterium Lamp

Holmium Oxide Filter

Flow Cell

Programmable slit

Grating

1024 element diode array

190 nm950 nmMinimized Noise in

Visible WL-Range

Automated wavelength verification

Fast optimization of sensitivity and resolution

Excellent wavelength resolution

More uptime > 2000h

nm230 240 250 260 270 280

Absorbance

00.10.20.30.40.50.6

(mAU)

1 nm

2 nm

4 nm8 nm

16 nmNoise level


November 2007

1.236

0.666

0.416

0.329

0.300

Peak Width

Peak Capacity

ResolutionData Rate

451.7120 Hz

291.17 10 Hz

160.675 Hz

552.0540 Hz

602.2580 Hz

min0.1 0.2 0.3 0.4 0.50

80Hz

PW=0.30sec

40Hz

PW=0.33sec

20HzPW=0.42sec

10HzPW=0.67sec

5HzPW=1.24secSample: Phenones Test MixColumn: Zorbax SB-C18, 4.6x30, 1.8umGradient:: 50-100%ACN in 0.3minFlow Rate: 5ml/min

80Hz versus 10Hz Data Rate• Peak Width: – 55%• Resolution: + 90%• Peak Capacity: + 120%• App. Column Eff.: + 260%

Influence of the detector rate on resolutionDAD SL and MWD SL


November 2007

ColumnSample

TempFlow

WLEluent

Injection

SB-C18 1.0x50 Iso Std +H2NCSNH2250.1 ml/min254,4; -/-80/20 H2O/ACN1 ul

New Drilled Flow Cell for DAD/MWDMinimized Dispersion for Maximum Resolution and SensitivityImproved flush-out behavior of the new standard flow cell minimizes peak tailing thereby increasing peak heights and resolution.

Ceramic Ring for thermal de-coupling

“Drill” Design of Inlet and Outlet for faster flush-out


November 2007

1200 Series Rapid Resolution LC- summary

1. Speed20X faster than conventional HPLC

2. Resolution60% higher resolution than conventional HPLC

3. Highest FlexibilityRRLC & standard methods, standard & narrow bore columnsBroad range of RRHT columns Fast method transfer: “HPLC to RRLC in 5 minutes”

4. Highest throughput 2000 Samples/Day on HT-configuration

5. Best usability on proven industry design More uptime, serviceability LC diagnostics, Intelligent and Compliance services New level of instrument control and data handling


November 2007

Agilent 6000 Series LC/MS Portfolio


November 2007

• Over 26,000 GC/MS, nearly 3,000 ICP-MS and 4000 LC/MS systems

5970B MSD

5930A

5975B5992A

1971 1976 1986 2006GC/MS 1971 - 2006

4500

ICP-MS 1994 - 2004

7500ce

1994 2004

LC/MSD, LC/MSD Ion Trap LC/MSD TOF

1996, 2000 2003, 2006LC/MS 1994 - 2006

Agilent’s 35 year heritage in mass spectrometry


November 2007

LC/MS Ion Sources•Widest choice of sourcesfrom any manufacturer

– Dual electrospray (ESI)– Dual nanospray– APCI– APPI– CE-TOF– PDF-MALDI

•Interchangeable between MS platforms•Multimode Source

– ESI and APCI combined in one source

– Simultaneous operation•HPLC-Chip/MS

– Nanospray made ease– Reproducible results– Sample processing on chip

Ionize Anything!


November 2007

Evolving the Agilent 6000 LC/MS Series A new and expanded portfolio for applications from life science to chemical analysis, combining exceptional performance and legendary Agilent reliability

LC/MSD Single Quad(enhanced)

6300 Series Ion Traps (enhanced)

6210 & 6220 TOF (enhanced)

6410 QQQ

6510 & 6520 QTOF

New


November 2007

LC/MS Systems for Proteomics/Metabolomics

QualitativeQualitative QuantitativeQuantitative

6510 QTOF Ultimate versatility and analytical performance for profiling, ID, and characterization of proteins, peptides and metabolites

6210 TOFHigh throughput profiling of proteins and metabolites, intact protein analysis with high mass accuracy

6410 Triple QuadrupoleTargeted quantitative analysis with femtogram level sensitivity and unbeatable price

6300 Series Ion Traps Popular high sensitivity protein ID system with MSn and new ETD capability for PTM analysis

HPLC-Chip “Powered”High resolution, high sensitivity nanospray LC/MS made convenient and easy


November 2007

LC/MS Systems for Drug Discovery, Developmentand QC/QA


6510 QTOF Ultimate MS/MS system value with high analytical performance for accurate mass based structural elucidation, and metabolite ID in drug discovery

6210 TOFHigh throughput walk up accurate mass system for compound screening and confirmation

6410 Triple QuadrupoleHigh sensitivity MS/MS system for quantitative analysis in drug metabolism and pharmacokinetic studies (DMPK)*

*21CFR Part 11 compliant software available late 20066300 Series Ion Traps

High sensitivity MSn workhorse for compound identification and structural elucidation in drug discovery and early ADMET

6000 Series Single QuadEasy to use high selectivity MS system for routine quantitationand use in QC/QA


November 2007

LC/MS Systems for Food, Environmental, Forensicand Clinical


6510 QTOF Ultimate MS/MS system value with high analytical performance for accurate mass based structural studies and identification of small molecules

6210 TOFHigh throughput walk up accurate mass system for screening and compound confirmation

6410 Triple QuadrupoleHigh sensitivity MS/MS system for targeted quantitative analysis in food safety, environmental, and clinical applications

6300 Series Ion Traps High sensitivity MSn system for the screening and identification of pesticide residues and unknowns in food and environmental studies

1200 Series Single QuadEasy to use, inexpensive, high selectivity MS system for screening and routine quantitation


November 2007

Single Quadrupole LC/MSMS Detection for Rapid Resolution Chromatography

6100 Series – Single Quad – NEW Performance…Fast Polarity Switching (10 pos + 10 neg spectra/sec)

Fast response for maximum chromatographic efficiency

Reduced Run to Run cycle time (<10 sec inter-run delay)

Advanced signal processing for enhanced sensitivity

HPLC-Chip supports research in microfluidics

Focus on Highest Throughput and Data Quality


November 2007

High Speed SeparationsMaximizing Chromatographic and MS Performance

min0.1 0.2 0.3 0.4 0.5 0.6 0.7

mAU

-5

0

5

10

15

20

25

DAD1 A, Sig=272,4 Ref=360,80 (G:\CHEM32 DATA\ASMS\ULTRASCPNMM0002.D)

0.149

0.250 0.4

32

0.668

min*0.1 0.2 0.3 0.4 0.5 0.6 0.7

0

20000

40000

60000

80000

100000

120000

140000*MSD1 BPC, MS File (G:\CHEM32 DATA\ASMS\ULTRASCPNMM0002.D) MM-ES+APCI, Fast Scan, Frag: 100, "PosScan"

0.149

0.251

0.432

0.667

min*0.1 0.2 0.3 0.4 0.5 0.6 0.70

5000

10000

15000

20000

25000

30000

35000

40000

*MSD2 BPC, MS File (G:\CHEM32 DATA\ASMS\ULTRASCPNMM0002.D) MM-ES+APCI, Fast Scan, Frag: 100, "NegScan"

0.150

0.251

0.432

0.668

UV 254 nm

Pos MultimodeES+APCI

Neg MultimodeES+APCI

0.91 sec FWHM9 scans/sec

0.87 sec FWHM9 scans/sec

0.95 sec FWHM4.5 spectra/sec

284 286 288

286.

0

286.

8

284.

8

284 286 288

286.

0

286.

8

284.

8 Proper chlorine isotope pattern

282 284 286

284.

0

284.

8

282.

8

282 284 286

284.

0

284.

8

282.

8 Proper chlorine isotope pattern

Analytes: Sulfamethizole, Sulfachloropyridizine, Sulfamethazine, SulfadimethoxineSource: Multimode Column: Zorbax 2.1 x 50mm, 1.8µM Flow Rate: 1.3 mL/min


November 2007

High Speed SeparationsHigh Throughput - Minimizing Run to Run Delay Time

Date acquired : Wed May 16 11:18:46 2007Date acquired : Wed May 16 11:19:56 2007Date acquired : Wed May 16 11:21:06 2007Date acquired : Wed May 16 11:22:16 2007Date acquired : Wed May 16 11:23:27 200710 second inter run time !!

1.00.2 0.4 0.6 0.8

0.16

1

0.34

60.

400

0.51

0

0.57

5

0.63

00.

658

0.82

7

1.00.2 0.4 0.6 0.8

0.34

60.

400

0.51

0

0.57

6

0.63

00.

657

0.82

6

Run 1 Run 2


November 2007

Agilent 6410 Triple Quad LC/MS

Best Price-Performance for Quantitative LC/MS• Femtogram sensitivity with excellent reproducibility

• 30-50% Lower price than comparable competitor instruments

• Autotune that truly works and ensures top performance

• New quantification software with peerless “intelligence” handles tasks previously considered un-automatable

• Agilent reliability and ease-of-use

High End Performance with Unprecedented Cost of Ownership

For food safety, environmental, chemical, forensics, pharmaceutical and metabolomics markets


November 2007

High Sensitivity QQQ LC/MS:Reserpine in Coffee (3 MRMs) < 160 femtograms!

20pg

10pg

5pg

2.5pg

1.25pg0.63pg0.32pg0.16pgBlank

1.25pg

0.63pg

0.32pg

Blank

0.16pg


November 2007

Agilent 6510 and 6520 QTOF LC/MS

Capture the most information from complex samples

• 10X sensitivity advantage (Attomole MS and MS/MS sensitivity)

• Wide in-scan dynamic ranges covers 3.5 decades

• Fast scan speeds (20/s) at up to 15,000 Resolving Power

Easy and confident identification of unknowns

• 2X typical mass accuracy (1 to 2 ppm MS and 5 ppm MS/MS)

• Automated reference mass introduction and calibration

• Autotune saves time and enhances performance

Ion-trap sensitivity, speed, and accurate mass that redefines the QTOF LCMS category


November 2007

High Sensitivity QTOF Performance:Data-Dependent MS/MS: 2 fmol on column BSA Digest

Base Peak Chromatogram

Spectrum Mill Data Base Search Results


November 2007

2x10

0

0.5

1

1.5

2

2.5

3

Abundance vs. Mass-to-Charge (m/z)50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000 1050 1100 1150 1200

+MS (4.600-4.685 min) MS/MS(0.0000 m/z) chip_LC_300ATMOLEbsa_ms2_1_.d (BP=479) Sub

251.1486

110.0690

653.3593

1055.5508594.2729796.3439354.6828 956.4929 1168.6293529.8208

[M+2H]2+b2

y2

a2

y1

H

I/L

b4 y6 b8 y8b7 b9

y9y10

b10

b5

MS/MS (K)HLVDEPQNLIK(Q) at

300attomole

3x10

0.5

1

1.5

2

2.5

3

3.5

4

4.5

5

5.5

6

Abundance vs. Acquisition Time (Min)0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5

+EIC (653.3500 m/z); from chip_lc_200ATMOLE_bsa_IRM_450nL2.d

1

HPLC-Chip and 6510 QTOF Sets New Standard in Protein Identification

(K)HLVDEPQNLIK(Q)From BSA digestPre-cursor trace

Unprecedented MS and MS/MS sensitivity rivals Ion Traps at attomole levels


November 2007

• Industry leading mass accuracy (1-2 ppm) and dynamic range

Highest throughput in small molecule applications• Multimode source: 2x increase in throughput• Faster scanning (40 scans/sec)• Fast polarity switching (1 sec) • Easy-access SW enables true “walk-up” use

Best sensitivity / reproducibility / productivity for complex sample analysis

• Increased Resolving Power – 10K at 100m/z, 16K at 1000 m/ztypical

• HPLC-Chip compatible• Mass Profiling and BioConfirmation Software

Agilent 6210 & 6220 TOF LC/MS


November 2007

TOF and Fast Chromatography 20 Hz (20 spectra per second; 10 per mode)

Compound Low Frag error High Frag error 1. Sulfamethizole -1.44 -2.732. Sulfachloropyridazine -1.92 -2.173. Sulfamethazine -0.89 -0.444. Sulfadimethoxine 0.00 -0.55

XIC of +TOF MS: Experiment 2, from hilowfrag01.wiff Max. 3.8e5 cps.

0.04 0.08 0.12 0.16 0.20 0.24 0.28 0.32 0.36 0.40 0.44 0.48 0.52Time, min

0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

Rel. Int. (%

)

0.432

0.393

0.219 0.302

Fast scanning TOF provides simultaneous:

•High resolution high speed separation – 30 secs•High mass accuracy – 1-2 ppm•High information content – high frag CID•APCI and ESI for broader coverage


November 2007

High Throughput Chemical Library AnalysisAnalysis of 140 real screening compounds under ultra-fast RRLC/TOF conditions (90s cycle time 1000 samples/day):

2 outliers not shown, 16 compounds could not be ionized by ESI+

-5.00

-4.00

-3.00

-2.00

-1.00

0.00

1.00

2.00

3.00

4.00

0 20 40 60 80 100 120 140

Err

or [p

pm]

Results from automated formula confirmationreport – no manual interaction!

LC: Water/ACN(0.1%TFA), 5-100%B in 0.7min, 60°C, 1.5ml/minUV-Detection: 210 – 500 nm, 80Hz MS-Detection: 120-1200Da, 8Hz, Split 1:7.5 Injection: 1µl, online sample dilution by injector program (determined the cycle time!)


November 2007

Agilent 6340 Ion Trap LC/MS

State-of-the-Art 3D Trap:• 10X sensitivity improvement (4-8X trap capacity)

• 3X scan cycle time improvement

• HPLC Chip compatible

• Reliability

Now with improvements in:Post-Translation Modification Analysis Capabilities• New release of Spectrum Mill adds PTM capability to industry-best protein ID

database search SW

• Identify phosphorylation site location to amino acid sequence via Electron Transfer Dissociation (ETD) Technology


November 2007

Combined HPLC-Chip-6330 Ion Trap Performance

1 MS and 7 MS/MS per cycle

Black signal is EIC of 985.6 (shown earlier in HPLC-Chip performance)

Red signal is TIC All MS showing acquisition rate

Note that acquisition rate is sufficient to accurately sample peak many time – thus could detect many co-eluting peaks

16.6 16.7 16.8 16.9 17.0 Time [min]0

1

2

3

x109

Intensity 14 MS and 98 MS/MS over peak

16.6 16.7 16.8 16.9 17.0 Time [min]16.6 16.7 16.8 16.9 17.0 Time [min]0

1

2

3

x109

Intensity

0

1

2

3

x109

Intensity 14 MS and 98 MS/MS over peak


November 2007

ETD: New Ionization Method for PTM Analysis:CID and ETD on TTHyGSLPQK [M+3H]3+ = m/z 404.7

147.1 186.8226.1

244.2

372.3

392.5

411.6

460.1485.2

505.2

560.7

629.3673.1

709.1727.1 840.3

0.00

0.25

0.50

0.75

1.00

1.25

4x10

200 400 600 800 1000 1200 m/z

y3

CID

2+

220.0

259.0

313.1339.1

357.2

402.6

469.1497.6

519.7556.3

606.7

657.2

676.1 718.2

744.2

775.2

805.1

823.3

856.2

872.4 938.4

954.4

993.4

1039.4

1082.4

1150.5

1196.6

1213.6

0

1000

2000

3000

4000

5000

200 400 600 800 1000 1200m/z

z8+1

z7+1

z6+1

z5+1z4+1z2+1

c2

c3

c4c5 c6

c9

ETD3+

1+

y1

y8

b7-18+2

b7b6

b6-18+2

MH+-36+3

Y-PO3HGS L

CID: y and b fragment ions

ETD: z and c fragment ions and works best with +3 charged ions


November 2007

Agilent LC/MS: Innovating Across the Whole System

• Dual electrospray (ESI)• Dual nanospray• APCI• APPI• CE-TOF• PDF-MALDI• Multimode

Widest range of ion sources

• 1200 LC• More than 900 columns

– MARS-Immunodepletion

– mRP• HPLC-Chip • Off-Gel Electrophoresis

Coming

World-leading separations

Easy and flexiblesoftware

• Shared MS SW Workstation

• Application SW• ECM• LIMS• Links to third parties

and customer IT


November 2007

1200 LC and 6000 Series LC/MS systems& 7890 GC and 5975C MSD systems

20 - agilent lc and lcms portfolio overview

Documents