Why Dextranase L ‘Amano’?Technical presentation

Apr-13

2 points to consider!

• Powerful! (very high endo activity)-> Dextranase L ‘Amano’ works rapidly and efficiently on Dextran during the process-> Dextranase L ‘Amano’ dosage can be adjustable at the factory depending on the purpose

• PURE! (very high purity)-> Dextranase L ‘Amano’ helps better crystallization

How powerful??

• Very quick Dextran hydrolysis• DP2 and DP3 production

Dextranase L ‘Amano’

0

20

40

60

80

100

0 5 10 15 20 25 30

Amou

nt of

suga

r com

pone

nts (

%)

time (min)

The balance of sugar componentｓ,10,000 ppm Dextran, after 50oC, 2ppm DextranaseL treatment.

Dextranhigher DP7DP6DP5DP4DP3DP2DP1

Tangible Advantages

• Better crystalized sugar• Gain better yield • Rapid reduction of viscosity• Higher productivity in the capacity• Shorter process time

Other advantages

• Easier maintenance of the equipment• Prevents clogging in the event of interruption

of the process.• Energy saving• Lower content of Dextran in waste water

What we observed?Voices from the market…

• Quality improvement!– Lower dextran content in the process -> less consumption of other chemicals -> lower quantity of waste water– Better particle distribution (medium size) of raw sugar – Better and solid crystal in raw sugar

• Yield improvement– Raw sugar yield increase up to 5-7%

• Save energy– Lower consumption of calcium carbonate– Lower quantity of waste water– Lower energy against raw sugar production / Mt

• Trouble shooting! (to add Dextranase just before re-start of the process)

– Longer life of filter cloth– Lower sugar loss

Crystallization

• Better crystal

Irregular crystal

Decrease of irregular crystals, increase of medium size crystals.

Delivery Cleaning Cutting DDS Preliming

Liming heating 1st

CarbonationHeat Filtr.1st Carbon.

juice

1st

Clearjuice

2ndCarbon.

Sulfur. Evap.Filtr Sulfiedjuice

Filtr. 2nd

Clearjuice

1st

Cryst.1st

Sepr.1st

Molas.2nd

Cryst.2nd

Sepr.2nd

Molas.3rd

Cryst.3rd

Sepr.FinalMolasses

Dextranase 2ppmCa(OH)2 　 2-4% less

CO2

FP

CO2 SO2

Raw sugar

Syrup

Diffusion juice Preliming juice145→30ppm

21→11ppm

122→52ppm

957→690ppm

Lower long crystal, mid size particle 77→80%, lower colour 65→44

Sugar loss2.79→2.43%

（ water consump. 23% lowerLower energy consump. (1770→1651 GJ）

Lower Turbidity, colour

Lower turbidity, colourLower turbidity, colour

Cake

38ppm

Glucose/Lactate analyzer

124ppm 239ppm

Dextranase

Mingler

Diffuser

DDS Preliming

Lactate value

137ppm

・ We assume the infection of Leuconostoc during the process since the lactate value is increased.・ We are checking the relationship between the value and dextran amount

(Info) There are 2 types of infectionBacteria and fungi•Comes normally during the storage of raw material in hot condition, which leads to lower yield of sugar.•Normally a carbamate based microbiocide specially formulated to inhibit growth of bacteria and fungi in cane sugar mills. Sodium dithiocarbamate, Sodium diethydlthiocarbamate and alkyl dimethyl benzyl ammonium are used in some countries.

Leuconostoc Mesnteroides•Under cold weather, Leuconostoc Mesnteroides makes dextran during the process, which causes filter problem and poor sugar quality (crystallization, fineness etc.)•An organic antimicrobial based on dual antibiotics with special ingredient effective for bacteriostatic and bactericidal action, Sodium dithiocarbamate is also used.•Through our experience in Japan, only Dextranase is used controlling MK value during the process (no anti-biotic agents at all).

Contact us!Amano Enzyme Europe Ltd.

Swiss BranchWTC Lugano

CH-6982 Agno+41 – 91- 604 6450

sugawara@amano.co.uk