waters desi system operator's guide

DESI SystemOperator’s Guide

715004701/Revision A

Copyright © Waters Corporation 2015All rights reserved

January 14, 2015, 715004701 Rev. APage ii

General Information

Copyright notice

© 2015 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.

The information in this document is subject to change without notice and should not be construed as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use. For the most recent revision of this document, consult the Waters Web site (waters.com).

Trademarks

Waters, “THE SCIENCE OF WHAT’S POSSIBLE.”, ESCi, UPLC, Xevo, and MassLynx are registered trademarks of Waters Corporation and IntelliStart, LockSpray, NanoFlow, NanoLockSpray, StepWave andT-Wave, are trademarks of Waters Corporation.

Microsoft, Windows, Word, Excel, and PowerPoint are registered trademarks of Microsoft Corporation in the United States and/or other countries.

PEEK is a trademark of Victrex plc.

TaperTip is a trademark of New Objective, Inc.

Other registered trademarks or trademarks are the sole property of their owners.

Customer comments

Waters’ Technical Communications organization invites you to report any errors that you encounter in this document or to suggest ideas for otherwise improving it. Help us better understand what you expect from our documentation so that we can continuously improve its accuracy and usability.

We seriously consider every customer comment we receive. You can reach us at [email protected].

January 14, 2015, 715004701 Rev. APage iii

Contacting Waters

Contact Waters with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail.

Safety considerations

Some reagents and samples used with Waters instruments and devices can pose chemical, biological, or radiological hazards (or any combination thereof). You must know the potentially hazardous effects of all substances you work with. Always follow Good Laboratory Practice, and consult your organization’s standard operating procedures.

Safety hazard symbol notice

Documentation needs to be consulted in all cases where the symbol is used to find out the nature of the potential hazard and any actions which have to be taken.

Waters contact information:

Contacting medium Information

Internet The Waters Web site includes contact information for Waters locations worldwide. Visit www.waters.com.

Telephone and fax From the USA or Canada, phone 800 252-4752, or fax 508 872 1990.For other locations worldwide, phone and fax numbers appear in the Waters Web site.

Conventional mail Waters Corporation34 Maple StreetMilford, MA 01757USA

January 14, 2015, 715004701 Rev. APage iv

Considerations specific to the DESI

Power cord replacement hazard

High temperature hazard

High voltage hazard

Crush hazards

Hazards associated contamination

Warning: To avoid electric shock, use the SVT-type power cord in the United States and HAR-type (or better) in Europe. The main power cord must only be replaced with one of adequate rating. For information regarding what cord to use in other countries, contact your local Waters distributor.

Warning: To avoid burn injuries, before performing maintenance operations that involve handling components inside the mass spectrometer's ion source, allow the source interior to cool.

Warning: • To avoid electric shock, do not remove the mass spectrometer’s

protective panels. The components they cover are not user-serviceable.

• To avoid nonlethal electric shock when the instrument is in Operate mode, avoid touching the areas marked with the high voltage warning symbol. To touch those areas, first put the instrument in Standby mode.

Warning: To avoid crush injury, be careful when working near the moving parts of the DESI.

Warning: To avoid personal contamination with biohazards, toxic materials, and corrosive materials, wear chemical-resistant gloves during all phases of instrument decontamination.

Warning: To avoid puncture injuries, handle syringes, fused silica lines, and borosilicate tips with extreme care.

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When you remove the instrument from use to repair or dispose of it, you must decontaminate all of its vacuum areas. These are the areas in which you can expect to encounter the highest levels of contamination:

• Source interior• Waste tubing• Exhaust system

The need to decontaminate other vacuum areas of the instrument depends on the kinds of samples the instrument analyzed and their levels of concentration. Do not dispose of the instrument or return it to Waters for repair until the authority responsible for approving its removal from the premises specifies the extent of decontamination required and the level of residual contamination permissible. That authority must also prescribe the method of decontamination to be used and the appropriate protection for personnel undertaking the decontamination process.

You must handle items such as syringes, fused silica lines, and borosilicate tips used to carry sample into the source area in accordance with laboratory procedures for contaminated vessels and sharps. To avoid contamination by carcinogens, toxic substances, or biohazards, you must wear chemical-resistant gloves when handling or disposing of used oil.

Bottle placement prohibition

FCC radiation emissions noticeChanges or modifications not expressly approved by the party responsible for compliance, could void the users authority to operate the equipment. This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.

Electrical power safety noticeDo not position the instrument so that it is difficult to operate the disconnecting device.

Equipment misuse noticeIf the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

Safety advisoriesConsult Appendix A for a comprehensive list of warning advisories and notices.

Prohibited: To avoid equipment damage caused by spilled solvent, do not place reservoir bottles directly atop an instrument or device or on its front ledge. Instead, place the bottles in the bottle tray, which serves as a secondary containment in the event of spills.

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Operating this DESI ion source

When operating this DESI ion source, follow standard quality-control (QC) procedures and the guidelines presented in this section.

Applicable symbols

Symbol Definition

Manufacturer

Date of manufacture

Authorized representative of the European Community

Confirms that a manufactured product complies with all applicable European Community directives

or

Australia EMC compliant

Confirms that a manufactured product complies with all applicable United States and Canadian safety requirements

Consult instructions for use

Supply ratings

Electrical and electronic equipment with this symbol may contain hazardous substances and should not be disposed of as general waste.For compliance with the Waste Electrical and Electronic Equipment Directive (WEEE) 2012/19/EU, contact Waters Corporation for the correct disposal and recycling instructions.Serial number

Part number catalog number��

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Audience and purposeThis guide is for operators with high levels of experience. The DESI ion source should not be used by anyone who has not been trained adequately. This manual gives an overview of the DESI ion source, and explains how to prepare it, change its modes of operation, and maintain it.

Intended use of the DESI ion sourceThe DESI ion source is designed for use with XEVO G2-XS and SYNAPT G2-Si mass spectrometers. The source offers automated sample positioning and the full advantage of desorption electrospray ionization technology. When used with a mass spectrometer, the DESI source eliminates the need for complex and time-consuming sample preperation.

DESI ion source instrumentation is not intended for use as a medical device. It is intended for use only in a laboratory environment in non-medical applications.

The DESI ion source must not be used in human experimentation or be applied to living humans in any way.

The efficacy of DESI has been demonstrated in many applications, from explosives to proteomics.

See Appendix B for information about operating the source.

EMC considerations

Canada spectrum management emissions noticeThis class A digital product apparatus complies with Canadian ICES-001.

Cet appareil numérique de la classe A est conforme à la norme NMB-001.

ISM Classification: ISM Group 1 Class AThis classification has been assigned in accordance with IEC CISPR 11 Industrial Scientific and Medical (ISM) instruments requirements. Group 1 products apply to intentionally generated and/or used conductively coupled radio-frequency energy that is necessary for the internal functioning of the equipment. Equipment defined as Class A is suitable for use in all establishments other than domestic establishments. Class A equipment can be directly connected to a low-voltage power-supply network that supplies buildings used for domestic purposes.

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EC authorized representative

Waters CorporationStamford AvenueAltrincham RoadWilmslow SK9 4AX UK

Telephone: +44-161-946-2400Fax: +44-161-946-2480Contact: Quality manager

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January 14, 2015, 715004701 Rev. APage x

Table of Contents

General Information .................................................................. iii

Copyright notice ......................................................................................... iii

Trademarks ................................................................................................ iii

Customer comments ................................................................................... iii

Contacting Waters ...................................................................................... iv

Safety considerations .................................................................................. ivSafety hazard symbol notice.............................................................. ivConsiderations specific to the DESI..................................................... vFCC radiation emissions notice........................................................... viElectrical power safety notice............................................................. viEquipment misuse notice .................................................................. viSafety advisories ............................................................................. vi

Operating this DESI ion source .................................................................... viiApplicable symbols.......................................................................... viiAudience and purpose .................................................................... viiiIntended use of the DESI ion source................................................. viii

EMC considerations ................................................................................... viiiCanada spectrum management emissions notice ................................ viiiISM Classification: ISM Group 1 Class A ............................................ viii

EC authorized representative ........................................................................ ix..................................................................................................... ix

1 Desorption Electrospray Ionisation (DESI) ion source ............. 13

1.1 Description .............................................................................................. 14

1.2 Handling .................................................................................................. 15

2 The Waters spray head ............................................................ 19

2.1 Waters spray head assembly ..................................................................... 192.1.1 Installing the Waters spray head........................................................ 202.1.2 Removing the Waters spray head....................................................... 20

2.2 Maintenance ............................................................................................ 202.2.1 Replacing the fused silica emitter tip .................................................. 212.2.2 Cleaning the Waters spray head ........................................................ 25

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3 Maintenance Procedures .......................................................... 27

3.1 Maintenance schedule ............................................................................... 27

3.2 Spare parts .............................................................................................. 283.2.1 Parts catalogue ............................................................................... 28

3.3 Replacing fuses ........................................................................................ 28

3.4 Spray-head nozzle/emitter replacement ....................................................... 28

3.5 Spray head cleaning .................................................................................. 293.5.1 Prosolia spray head.......................................................................... 293.5.2 Waters spreay head ......................................................................... 29

A Safety Advisories ..................................................................... 31

A.1 Warning symbols ...................................................................................... 31A.1.1 Specific warnings ............................................................................ 32

A.2 Notices ................................................................................................... 34

A.3 Bottles Prohibited symbol .......................................................................... 34

A.4 Required protection .................................................................................. 35

A.5 Warnings that apply to all Waters instruments and devices ............................. 35

A.6 Warnings that address the replacing of fuses ................................................ 38

A.7 Electrical and handling symbols .................................................................. 40A.7.1 Electrical symbols............................................................................ 40A.7.2 Handling symbols ............................................................................ 41

B Prosolia DESI Source 2D, Installation and Operation Manual ... 43

B.1 The Prosolia DESI manual .......................................................................... 43

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1 Desorption Electrospray Ionisation (DESI) ion source

This chapter describes the DESI ion source. For a description of the control and operation of the DESI ion source, see Appendix B.

Warning: To avoid the escape of vaporised materials, from the DESI source, which is not gas tight, to the laboratory atmosphere, observe the appropriate safety guidelines for the material being sampled.

January 14, 2015, 715004701 Rev. A

1.1 Description

Desorption Electrospray ionisation (DESI), is a pneumatically assisted electrospray technique, in which a solvent is directed towards the sample of intrest. The resultant ions are sampled directly, under ambient conditions, in the Mass Spectrometer.

These variables can help achieve optimal spectral results;

• Solvent• Gas flow rate• Amount of applied voltage• Spray angle• Ion uptake angle• Distances involved in aligning the spray• Sample type

An expanded publications list related to DESI is available on the Prosolia website, www.prosolia.com.

Solvent

N2

Spray nozzle

SampleSprayDesorbed ions

Inlet to mass spectrometer

Freely moving sample stage

High voltage source

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1.2 Handling

Warning: To avoid burn injuries, do not touch the ion block when the instrument is in use or when it has been in recent use.

Warning: To avoid puncture wounds, do not touch the sharp tips of the spray capillaries or emitter.

Warning: To avoid electric shock, ensure that the high voltage is switched off before touching the source‘s spray head. The ion block and the spray head must be installed before the high voltage is switched on.

Warning: To avoid injury, from the sudden release of highly pressurised Nitrogen, depressurize before working on the source head or spray capillaries.


Notice: To avoid damaging the DESI source, do not lift it by its handles. The handles are intended only for lifting the source‘s lid.

Emitter

High voltage input

Solvent input

Gas input

Prosolia spray head

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The Waters spray head is designed to operate with lower gas and solvent flow rates than previous models. This will allow for greater accuracy of analysis.

When installing, removing or handling the DESI unit, care must be taken to prevent damage.

Particular care should be taken to make sure damage does not occur to:

• The emitter/spray head• Fused silica• Solvent tubing• Gas connections and tubing• Ion transfer capillary (Bazooka).

H.V. connection

Solvent fitting

Gas fitting

Mounting bracket

Gas cone

PEEK body

Waters spray head

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When moving the spray-head arm, ensure that the tubing and cabling does not become stretched and the routing is not altered.

When moving the tray, ensure that the ion transfer capilliary clears the tray‘s surface.

When necessary, lift the DESI source from the instrument by supporting it from its underside.

When preparing tubing, ensure that all cuts are perpindicular or square to the tubing.

Notice: To avoid damaging the DESI source, do not lift it by its handles. The handles are intended only for lifting the source‘s lid.

Ion block Ion capilliary Spray-head arm, folded for access

Spray head

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January 14, 2015, 715004701 Rev. A

2 The Waters spray head

This chapter describes the installation, removal and maintenance of the Waters spray head.

2.1 Waters spray head assembly

The Waters spray head is shipped preloaded with a fused silica emitter. Before using the source, you must adjust the length of the emitter that protrudes from the nozzle by turning the nozzle clockwise using the 4-mm wrench provided with the source. The emitter must protrude approximately 1-mm beyond the tip of the spray nozzle.

You must also connect tubing for the spray solvent and nitrogen nebulizing gas to the spray head. The tubing and fittings necessary to make these connections are provided with the source.

The spray head uses TaperTip® emitters, which are precut to 40-mm and provide an identical configuration when replaced. Thus they are designed to provide reproducability, robustness, and high performance.

Notice: There are differences between the way the Waters spray head and the Prosolia spray head are set up and operated. The Waters spray head is initially installed by a Waters technician. If the Prosolia spray head is to be installed after the initial installation, you must refer to the Prosolia documentation (at Appendix B) for set up and gas pressure information.

H.V. connection

Solvent fitting

Gas fitting

Mounting bracket

Gas cone

PEEK body

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2.1.1 Installing the Waters spray head1. Turn the source out of operate and power off the DESI control unit.

2. Open the cover, and remove it, if necessary.

3. Lower the DESI stage, and move the arm to the maintenance position.

4. On the new spray head, use a 4-mm wrench to turn the gas cone clockwise until the emitter tip protrudes by approx 0.5-mm to 1-mm.

5. Install the new mounting bracket and spray head assembly.

6. Install the solvent fitting to the spray head.

Note: The supplied 360 µm o.d. fitting for the solvent capillary does not require a sleeve. Capilliaries whose outer dimensions are larger or smaller than 360 µm require the use of alternative fittings. Refer to the Waters website for information about spare parts.

7. Install the high voltage connection onto the spray head.

8. Fit the gas fitting to the spray head.

9. Install the cover, if necessary.

2.1.2 Removing the Waters spray head.1. Turn the source out of operate and power off the DESI control unit.

2. Disconnect the voltage connection.

3. Turn off the gas supply.

4. Disconnect the gas fitting.

5. Disconnect the solvent fitting.

6. Remove the spray head assembly, including the mounting bracket.

2.2 Maintenance

The Waters spray head requires no routine maintenance procedures. Nevertheless, depending on usage, you should perform the following procedures periodically:



Warning: To avoid burn injuries, do not touch the ion block when the instrument is in use or when it has been in recent use.


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• Replace the fused silica emitter tip• Clean the Waters spray head

2.2.1 Replacing the fused silica emitter tipReplace the fused silica emitter tip when any of the following conditions apply:

• The Waters spray head is dismantled.• When you suspect the use of contaminated solvent.• You suspect a regularly used instrument is giving erroneous results.

To remove the emitter tip:

1. Turn the source out of operate and power off the DESI control unit.

2. Disconnect the voltage, gas and solvent inputs.

3. Lower the stage.

4. Swing the arm to access the spray head.

5. Remove the spray head.

6. Remove the metal rear section from the body of the spray head.


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7. Use wrenches to loosen the front section of the metal assembly.

8. Remove the emitter tip.

To install a new fused silica emitter-tip:

1. Remove the metal assembly from the PEEK body.

2. Use a wrench to seperate the two parts of the metal assembly.

3. Remove and keep the ferrule.


Front metal part Rear metal partFerrule

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4. Screw the positioning nut onto the rear metal part.

5. Insert a new emitter, cut-end first, through the front, metal part and through the ferrule.

6. Install the front, metal part on the rear metal part.

7. Ensure that the emitter found its correct place against the positioning nut.

8. Use wrenches to tighten together the front, metal part and the rear, metal part.

Positioning nut

Apply wrenches here

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9. Install the metal assembly in the PEEK body.

10. Hold the PEEK body so that it is horizontal, and install the gas cone on the front, metal part.

11. Use a wrench to wind the gas cone into the body until the emitter tip protrudes by approx 1.0-mm.

12. Remove the positioning nut.

13. Ensure that the DESI control unit is off and the source is out of operate.

14. Install the spray head.

Gas cone

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2.2.2 Cleaning the Waters spray headClean the Waters spray head when any of the following conditions apply:

• The fused silica emitter tip is removed or replaced.• The solvent is changed.• When you suspect the use of contaminated solvent.

You clean the system by pumping solvent through it without using a lockmass.

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January 14, 2015, 715004701 Rev. A

3 Maintenance Procedures

Keep to a maintenance schedule, and perform maintenance as required and described in this chapter.

3.1 Maintenance schedule

The following table lists maintenance operations that ensure optimum instrument performance.

Perform replacement tasks as necessary. Those appearing below apply to instruments that normally receive moderate use.

See Appendix A for safety advisory information.

Warning: To avoid personal contamination from contact with biohazards or toxic materials, always wear clean, chemical-resistant, powder-free gloves when performing maintenance operations.

Warning: To prevent injury, always observe Good Laboratory Practice when handling solvents, changing tubing, or operating the instrument. Know the physical and chemical properties of the solvents used (see the Material Safety Data Sheets for the solvents in use).

Warning: To avoid electric shock:• do not remove the instrument’s panels. There are no user-serviceable

items inside the instrument.• ensure that the instrument is in Standby mode before begining any

maintenance operation.

Maintenance schedule:

Procedure Frequency Information...

Clean the instrument case. As required. Do not clean with abrasive or solvents.

Clean the source components. When sensitivity decreases to unacceptable levels.

See Appendix B

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3.2 Spare parts

To ensure that the system operates as designed, use only Waters Quality Parts. Visit www.waters.com/wqp for information about Waters Quality Parts, including how to order them.

3.2.1 Parts catalogueSee Appendix B for a list of Waters-approved Prosolia parts.

For Waters consumable parts refer to Waters.com.

3.3 Replacing fuses

Under normal operation, the fuses protecting the DESI control electronics do not need replacing. Should a fuse for the control electronics open, replace it according to the procedure at Appendix B.

3.4 Spray-head nozzle/emitter replacement

A qualified technician must replace the spray nozzle/emitter. Improper installation can adversley affect the quality of results.

For details about replacing the spray-head nozzle/emitter, see Appendix B (Prosolia) or Chapter 2.1.2 (Waters).

Spray head cleaning. When sensitivity decreases to unacceptable levels.

See Appendix B (Prosolia) or Chapter 2.2.2 (Waters)

Spray head nozzle/Emitter replace.

As required on condition.

See Appendix B (Prosolia) or Chapter 2.1, (Waters).

Fuse replacement. As required. See Appendix B

Maintenance schedule: (continued)

Procedure Frequency Information...

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3.5 Spray head cleaning

You can clean the spray head of the DESI source if you suspect chemical contamination of the spray head. When doing so, avoid damaging the spray head‘s o-ring seals. Simple cleaning of the outer surfaces involves wiping the spray head using a wipe saturated in methanol. Note that you must not submerge the spray head in solvents.

3.5.1 Prosolia spray headThoroughly cleaning the Prosolia spray head requires disassembly and removal of the o-ring seals. Except for the electrode assembly, you can clean all parts of the spray head by sonication in methanol. After removing the spray head from the source, follow the procedure in Appendix B to disassemble and clean the spray head.

3.5.2 Waters spreay headFor details about cleaning the Waters spray head, follow the procedure in Chapter 2.2.2

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January 14, 2015, 715004701 Rev. A

A Safety Advisories

Waters instruments and devices display hazard symbols that alert you to the hidden dangers associated with a product’s operation and maintenance. The symbols also appear in product manuals where they accompany statements describing the hazards and advising how to avoid them. This appendix presents the safety symbols and statements that apply to all of Waters’ product offerings.

A.1 Warning symbols

Warning symbols alert you to the risk of death, injury, or seriously adverse physiological reactions associated with the misuse of an instrument of device. Heed all warnings when you install, repair, or operate any Waters instrument or device. Waters accepts no liability in cases of injury or property damage resulting from the failure of individuals to comply with any safety precaution when installing, repairing, or operating any of its instruments or devices.

The following symbols warn of risks that can arise when you operate or maintain a Waters instrument or device or component of an instrument or device. When one of these symbols appear in a manual’s narrative sections or procedures, an accompanying statement identifies the applicable risk and explains how to avoid it.

Warning: (General risk of danger. When this symbol appears on an instrument, consult the instrument’s user documentation for important safety-related information before you use the instrument.)

Warning: (Risk of burn injury from contacting hot surfaces.)

Warning: (Risk of electric shock.)

Warning: (Risk of fire.)

Warning: (Risk of sharp-point puncture injury.)

Warning: (Risk of hand crush injury.)

Warning: (Risk of injury caused by moving machinery.)

Warning: (Risk of exposure to ultraviolet radiation.)

Warning: (Risk of contacting corrosive substances.)

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A.1.1 Specific warningsThe following warnings (both symbols and text) can appear in the user manuals of particular instruments and devices and on labels affixed to them or their component parts.

A.1.1.1 Burst warning

This warning applies to Waters instruments and devices fitted with nonmetallic tubing.

A.1.1.2 Mass spectrometer shock hazard

The following warning applies to all Waters mass spectrometers.

The following warning applies to certain mass spectrometers when they are in Operate mode.

Warning: (Risk of exposure to a toxic substance.)

Warning: (Risk of personal exposure to laser radiation.)

Warning: (Risk of exposure to biological agents that can pose a serious health threat.)

Warning: (Risk of tipping.)

Warning: (Risk of explosion.)

Warning: (Risk of high-pressure gas release.)

Warning: To avoid injury from bursting, nonmetallic tubing, heed these precautions when working in the vicinity of such tubing when it is pressurized:• Wear eye protection.• Extinguish all nearby flames.• Do not use tubing that is, or has been, stressed or kinked.• Do not expose nonmetallic tubing to compounds with which it is chemically

incompatible: tetrahydrofuran, nitric acid, and sulfuric acid, for example.• Be aware that some compounds, like methylene chloride and dimethyl

sulfoxide, can cause nonmetallic tubing to swell, significantly reducing the pressure at which the tubing can rupture.

Warning: To avoid electric shock, do not remove the mass spectrometer’s protective panels. The components that they cover are not user-serviceable.

Warning: To avoid harmless, static-like electric shock, ensure the mass spectrometer is in Standby mode before you touch any of its external surfaces that are marked with this high voltage warning symbol.

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A.1.1.3 Mass spectrometer flammable solvents warning

This warning applies to mass spectrometers performing an analysis that requires the use of flammable solvents.

A.1.1.4 Biohazard warning

The following warning applies to Waters instruments and devices that can process material containing biohazards, which are substances that contain biological agents capable of producing harmful effects in humans.

Warning: To prevent ignition of flammable solvent vapors in the enclosed space of a mass spectrometer’s ion source, ensure that nitrogen flows continuously through the source. The nitrogen supply pressure must not fall below 690 kPa (6.9 bar, 100 psi) during an analysis requiring the use of flammable solvents. Also a gas-fail device must be installed, to interrupt the flow of LC solvent should the nitrogen supply fail.

Warning: To avoid infection with potentially infectious, human-sourced products, inactivated microorganisms, and other biological materials, assume that all biological fluids that you handle are infectious. Specific precautions appear in the latest edition of the US National Institutes of Health (NIH) publication, Biosafety in Microbiological and Biomedical Laboratories (BMBL).Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials, and consult the biohazard safety representative for your organization regarding the proper use and handling of infectious substances.

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A.1.1.5 Biohazard and chemical hazard warning

These warnings apply to Waters instruments and devices that can process biohazards, corrosive materials, or toxic materials.

A.2 Notices

Notice advisories appear where an instrument or device can be subject to use or misuse that can damage it or compromise a non-clinical sample’s integrity (risks to clinical sample integrity are accompanied by warning symbols). The exclamation point symbol and its associated statement alert you to such risk.

A.3 Bottles Prohibited symbol

The Bottles Prohibited symbol alerts you to the risk of equipment damage caused by solvent spills.

Warning: To avoid personal contamination with biohazards, toxic materials, or corrosive materials, you must understand the hazards associated with their handling. Guidelines prescribing the proper use and handling of such materials appear in the latest edition of the National Research Council's publication, Prudent Practices in the Laboratory: Handling and Management of Chemical Hazards. Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials, and consult the safety representative for your organization regarding its protocols for handling such materials.

Notice: To avoid damaging the instrument’s case, do not clean it with abrasives or solvents.

Prohibited: To avoid equipment damage caused by spilled solvent, do not place reservoir bottles directly atop an instrument or device or on its front ledge. Instead, place the bottles in the bottle tray, which serves as secondary containment in the event of spills.

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A.4 Required protection

The Use Eye Protection and Wear Protective Gloves symbols alert you to the requirement for personal protective equipment. Select appropriate protective equipment according to your organization’s standard operating procedures.

A.5 Warnings that apply to all Waters instruments and devices

When operating this device, follow standard quality-control procedures and the equipment guidelines in this section.

Requirement: Use eye protection when refilling or replacing solvent bottles.

Requirement: Wear clean, chemical-resistant, powder-free gloves when handling samples.

Attention: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.

Important: Toute modification sur cette unité n’ayant pas été expressément approuvée par l’autorité responsable de la conformité à la réglementation peut annuler le droit de l’utilisateur à exploiter l’équipement.

Achtung: Jedwede Änderungen oder Modifikationen an dem Gerät ohne die ausdrückliche Genehmigung der für die ordnungsgemäße Funktionstüchtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems führen.

Avvertenza: qualsiasi modifica o alterazione apportata a questa unità e non espressamente autorizzata dai responsabili per la conformità fa decadere il diritto all'utilizzo dell'apparecchiatura da parte dell'utente.

Atencion: cualquier cambio o modificación efectuado en esta unidad que no haya sido expresamente aprobado por la parte responsable del cumplimiento puede anular la autorización del usuario para utilizar el equipo.

注意：未經有關法規認證部門允許對本設備進行的改變或修改,可能會使使用者喪失操作該設備的權利。

注意：未经有关法规认证部门明确允许对本设备进行的改变或改装，可能会使使用者丧失操作该设备的合法性。

주의: 규정 준수를 책임지는 당사자의 명백한 승인 없이 이 장치를 개조 또는 변경할 경우, 이 장치를 운용할 수 있는 사용자 권한의 효력을 상실할 수 있습니다.

January 14, 2015, 715004701 Rev. A

注意：規制機関から明確な承認を受けずに本装置の変更や改造を⾏うと、本装置のユーザーとしての承認が無効になる可能性があります。

Warning: Use caution when working with any polymer tubing under pressure:• Always wear eye protection when near pressurized polymer tubing.• Extinguish all nearby flames.• Do not use tubing that has been severely stressed or kinked.• Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated

nitric or sulfuric acids.• Be aware that methylene chloride and dimethyl sulfoxide cause nonmetallic

tubing to swell, which greatly reduces the rupture pressure of the tubing.

Attention: Manipulez les tubes en polymère sous pression avec precaution:• Portez systématiquement des lunettes de protection lorsque vous vous

trouvez à proximité de tubes en polymère pressurisés.• Eteignez toute flamme se trouvant à proximité de l’instrument.• Evitez d'utiliser des tubes sévèrement déformés ou endommagés.• Evitez d'utiliser des tubes non métalliques avec du tétrahydrofurane (THF) ou

de l'acide sulfurique ou nitrique concentré.• Sachez que le chlorure de méthylène et le diméthylesulfoxyde entraînent le

gonflement des tuyaux non métalliques, ce qui réduit considérablement leur pression de rupture.

Vorsicht: Bei der Arbeit mit Polymerschläuchen unter Druck ist besondere Vorsicht angebracht:• In der Nähe von unter Druck stehenden Polymerschläuchen stets Schutzbrille

tragen.• Alle offenen Flammen in der Nähe löschen.• Keine Schläuche verwenden, die stark geknickt oder überbeansprucht sind.• Nichtmetallische Schläuche nicht für Tetrahydrofuran (THF) oder

konzentrierte Salpeter- oder Schwefelsäure verwenden.Durch Methylenchlorid und Dimethylsulfoxid können nichtmetallische Schläuche quellen; dadurch wird der Berstdruck des Schlauches erheblich reduziert.

Attenzione: fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione:• Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero

pressurizzati.• Spegnere tutte le fiamme vive nell'ambiente circostante.• Non utilizzare tubi eccessivamente logorati o piegati.• Non utilizzare tubi non metallici con tetraidrofurano (THF) o acido solforico o

nitrico concentrati.• Tenere presente che il cloruro di metilene e il dimetilsolfossido provocano

rigonfiamenti nei tubi non metallici, riducendo notevolmente la pressione di rottura dei tubi stessi.

January 14, 2015, 715004701 Rev. A

Advertencia: se recomienda precaución cuando se trabaje con tubos de polímero sometidos a presión:• El usuario deberá protegerse siempre los ojos cuando trabaje cerca de tubos

de polímero sometidos a presión.• Si hubiera alguna llama las proximidades.• No se debe trabajar con tubos que se hayan doblado o sometido a altas

presiones.• Es necesario utilizar tubos de metal cuando se trabaje con tetrahidrofurano

(THF) o ácidos nítrico o sulfúrico concentrados.Hay que tener en cuenta que el cloruro de metileno y el sulfóxido de dimetilo dilatan los tubos no metálicos, lo que reduce la presión de ruptura de los tubos.

警告：當在有壓力的情況下使用聚合物管線時，小心注意以下幾點。• 當接近有壓力的聚合物管線時一定要戴防護眼鏡。• 熄滅附近所有的火焰。• 不要使用已經被壓癟或嚴重彎曲管線。• 不要在非金屬管線中使用四氫呋喃或濃硝酸或濃硫酸。要了解使用二氯甲烷及二甲基亞楓會導致非金屬管線膨脹，大大降低管線的耐壓能力。

警告：当有压力的情况下使用管线时，小心注意以下几点：• 当接近有压力的聚合物管线时一定要戴防护眼镜。• 熄灭附近所有的火焰。• 不要使用已经被压瘪或严重弯曲的管线。• 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸。要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀，大大降低管线的耐压能力。

경고: 가압 폴리머 튜브로 작업할 경우에는 주의하십시오.• 가압 폴리머 튜브 근처에서는 항상 보호 안경을 착용하십시오.• 근처의 화기를 모두 끄십시오.• 심하게 변형되거나 꼬인 튜브는 사용하지 마십시오.• 비금속(Nonmetallic) 튜브를 테트라히드로푸란(Tetrahydrofuran: THF) 또는

농축 질산 또는 황산과 함께 사용하지 마십시오.염화 메틸렌 (Methylene chloride) 및 디메틸술폭시드 (Dimethyl sulfoxide) 는 비금속 튜브를 부풀려 튜브의 파열 압력을 크게 감소시킬 수 있으므로 유의하십시오 .

警告：圧⼒のかかったポリマーチューブを扱うときは、注意してください。• 加圧されたポリマーチューブの付近では、必ず保護メガネを着用してください。• 近くにある火を消してください。• 著しく変形した、または折れ曲がったチューブは使用しないでください。• 非⾦属チューブには、テトラヒドロフラン(THF)や⾼濃度の硝酸または硫酸などを流さ

ないでください。塩化メチレンやジメチルスルホキシドは、非⾦属チューブの膨張を引き起こす場合があり、その場合、チューブは極めて低い圧⼒で破裂します。

Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

Attention: L’utilisateur doit être informé que si le matériel est utilisé d’une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d’être défectueuses.

January 14, 2015, 715004701 Rev. A

A.6 Warnings that address the replacing of fuses

The following warnings pertain to instruments and devices equipped with user-replaceable fuses. Information describing fuse types and ratings sometimes, but not always, appears on the instrument or device.

Finding fuse types and ratings when that information appears on the instrument or device

Vorsicht: Der Benutzer wird darauf aufmerksam gemacht, dass bei unsachgemäßer Verwendung des Gerätes die eingebauten Sicherheitseinrichtungen unter Umständen nicht ordnungsgemäß funktionieren.

Attenzione: si rende noto all'utente che l'eventuale utilizzo dell'apparecchiatura secondo modalità non previste dal produttore può compromettere la protezione offerta dall'apparecchiatura.

Advertencia: el usuario deberá saber que si el equipo se utiliza de forma distinta a la especificada por el fabricante, las medidas de protección del equipo podrían ser insuficientes.

警告：使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用，那麼該設備所提供的保護將被消弱。

警告：使用者必须非常清楚如果设备不是按照制造厂商指定的方式使用，那么该设备所提供的保护将被削弱。

경고 : 제조업체가 명시하지 않은 방식으로 장비를 사용할 경우 장비가 제공하는 보호 수단이 제대로 작동하지 않을 수 있다는 점을 사용자에게 반드시 인식시켜야 합니다 .

警告：ユーザーは、製造元により指定されていない方法で機器を使用すると、機器が提供している保証が無効になる可能性があることに注意して下さい。

Warning: To protect against fire, replace fuses with those of the type and rating printed on panels adjacent to instrument fuse covers.

Attention: pour éviter tout risque d'incendie, remplacez toujours les fusibles par d'autres du type et de la puissance indiqués sur le panneau à proximité du couvercle de la boite à fusible de l'instrument.

Vorsicht: Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetzen, deren Typ und Nennwert auf den Tafeln neben den Sicherungsabdeckungen des Geräts gedruckt sind.

Attenzione: per garantire protezione contro gli incendi, so8stituire i fusibili con altri dello stesso tipo aventi le caratteristiche indicate sui pannelli adiacenti alla copertura fusibili dello strumento.

January 14, 2015, 715004701 Rev. A

Finding fuse types and ratings when that information does not appear on the instrument or device

Advertencia: Para evitar incendios, sustituir los fusibles por aquellos del tipo y características impresos en los paneles adyacentes a las cubiertas de los fusibles del instrumento.

警告：為了避免火災，更換保險絲時，請使用與儀器保險絲蓋旁面板上所印刷之相同類型與規格的保險絲。

警告：为了避免火灾，应更换与仪器保险丝盖旁边面板上印刷的类型和规格相同的保险丝。

경고 : 화재의 위험을 막으려면 기기 퓨즈 커버에 가까운 패널에 인쇄된 것과 동일한 타입 및 정격의 제품으로 퓨즈를 교체하십시오 .

警告：火災予防のために、ヒューズ交換では機器ヒューズカバー脇のパネルに記載されているタイプおよび定格のヒューズをご使用ください。

Warning: To protect against fire, replace fuses with those of the type and rating indicated in the “Replacing fuses” section of the Maintenance Procedures chapter.

Attention: pour éviter tout risque d'incendie, remplacez toujours les fusibles par d'autres du type et de la puissance indiqués dans la rubrique "Remplacement des fusibles" du chapitre traitant des procédures de maintenance.

Vorsicht: Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetzen, deren Typ und Nennwert im Abschnitt "Sicherungen ersetzen" des Kapitels "Wartungsverfahren" angegeben sind.

Attenzione: per garantire protezione contro gli incendi, sostituire i fusibili con altri dello stesso tipo aventi le caratteristiche indicate nel paragrafo "Sostituzione dei fusibili" del capitolo "Procedure di manutenzione".

Advertencia: Para evitar incendios, sustituir los fusibles por aquellos del tipo y características indicados en la sección "Sustituir fusibles".

警告：為了避免火災，更換保險絲時，應使用「維護步驟」章節中「更換保險絲」所指定之相同類型與規格的保險絲。

警告：为了避免火灾，应更换 “ 维护步骤 ” 一章的 “ 更换保险丝 ” 一节中介绍的相同类型和规格的保险丝。

경고 : 화재의 위험을 막으려면 유지관리 절차 단원의 “ 퓨즈 교체 ” 절에 설명된 것과 동일한 타입 및 정격의 제품으로 퓨즈를 교체하십시오 .

警告：火災予防のために、ヒューズ交換ではメンテナンス項目の「ヒューズの交換」に記載されているタイプおよび定格のヒューズをご使用ください。

January 14, 2015, 715004701 Rev. A

A.7 Electrical and handling symbols

A.7.1 Electrical symbolsThe following electrical symbols and their associated statements can appear in instrument manuals and on an instrument’s front or rear panels.

Symbol Description

Electrical power on

Electrical power off

Standby

Direct current

Alternating currentAlternating current (3 phase)

Safety ground

Frame, or chassis, terminal

FuseFunctional ground

Input

Output

January 14, 2015, 715004701 Rev. A

A.7.2 Handling symbolsThe following handling symbols and their associated statements can appear on labels affixed to the packaging in which instruments, devices, and component parts are shipped.

Symbol Description

Keep upright!

Keep dry!

Fragile!

Use no hooks!

Upper limit of temperature

Lower limit of temperature

Temperature limitation

January 14, 2015, 715004701 Rev. A

January 14, 2015, 715004701 Rev. A

January 14, 2015, 715004701 Rev. A

B Prosolia DESI Source 2D, Installation and Operation Manual

This Appendix contains the Prosolia DESI Source 2D, Installation and Operation manual. This manual is not a Waters document.

B.1 The Prosolia DESI manual

The Prosolia DESI manual in this appendix is intended for reference purposes only and is subject to change without notice.

The manual includes the following chapters:

1. Introduction

2. Source Assembly

3. DESI ion source installation

4. DESI motion control box connections

5. Operation of DESI source

6. DESI ion source testing

7. Source maintenance

8. Spare parts

9. Troubleshooting

10. Where to get help

PN:P900‐16205‐01 Revision A.1

DESI 2D Ion Source Installation and Operation Manual

Waters Xevo Mass Spectrometers

Prosolia, Inc. www.prosolia.com

6500 Technology Center Drive Phone: 866‐241‐0239

Suite 200 Phone: 317‐275‐5794

Indianapolis, IN 46278 Fax: 317‐873‐3175

[email protected]

i

© Prosolia, Inc. 2015 All Rights Reserved.

The technical information contained in the DESI 2D Ion Source Installation and Operation Manual is

intended for reference purposes only and is subject to change without notice. All technical information

in this document is for reference purposes only. System configurations and specifications in this document

supersede all previous information received by the user.

Prosolia has endeavored to make every reasonable effort to supply complete and accurate information

regarding the installation and operation of the DESI 2D system. Prosolia, Inc. makes no representations

that this document is complete, accurate, or error‐free and assumes no responsibility for any errors,

omissions, damage, or loss that might result from any use of any information contained within this

document, even if the information in the document is followed properly.

No license or other rights under any patents, copyrights or trademarks owned by Prosolia or under which

Prosolia is licensed are granted or implied by the sale of the DESI 2D system. The customer shall not resell

or reverse engineer this equipment.

Ownership and use of the DESI Ion Source is subject to Prosolia's Standard Terms and Conditions, a copy

of which has been provided or is available upon request to Prosolia.

TRADEMARKS

PEEK is a trademark of Victrex plc.

Xevo™, Synapt™, and MassLynx™ are trademarks of Waters Corporation.

Swagelok® is a registered trademark of Swagelok Company.

Omni Slide™ is a trademark of Prosolia, Inc.

ii

Limited Warranty

Prosolia warrants to the Customer and any other end user of the Products manufactured by Prosolia that such Products will be substantially free

from defects in material and workmanship when properly installed, maintained and used in conformity with the guidelines provided in the

Product’s accompanying documentation. This limited warranty will be in effect for each Product for a period of one (1) year from the date of

invoice to the original Customer. This limited warranty excludes the following: (a) parts that are not defective at time of delivery but which

become defective by virtue of usage, including but not limited to normal wear, tear and replacement; (b) any damages or problems caused by

the failure to properly install, maintain or use the Products; (c) products or parts that have been repaired or altered by anyone other than Prosolia

or its authorized agents or affiliates; (d) expendable items including but not limited to capillaries, tubing, fittings, substrates, fuses, o‐rings, belts,

gaskets, cartridges, probe tips and chemical standards; (e) products not manufactured by Prosolia; or (f) software products from third parties

that are included with the Products or as optional add‐on modules or features ("Third Party Software"). THE FOREGOING WARRANTY IS INTENDED

SOLELY FOR THE BENEFIT OF THE FIRST CUSTOMER OF THE PRODUCTS AND IS NON‐ASSIGNABLE. PROSOLIA SPECIFICALLY DISCLAIMS ALL OTHER

WARRANTIES, EXPRESS OR IMPLIED INCLUDING, BUT NOT LIMITED TO, ANY IMPLIED WARRANTY OF MERCHANTABILITY, ANY IMPLIED

WARRANTY OF FITNESS FOR A PARTICULAR PURPOSE, AND ANY WARRANTY OF TITLE AND AGAINST INFRINGEMENT. PROSOLIA AND ITS

LICENSORS AND THEIR SUPPLIERS DO NOT WARRANT THAT THE PRODUCTS ARE OR WILL BE ERROR OR BUG FREE, OR THAT CUSTOMER'S USE OF

THE PRODUCTS WILL BE UNINTERRUPTED. PROSOLIA AND ITS LICENSORS AND THEIR SUPPLIERS ASSUME NO RESPONSIBILITY FOR THE PROPER

INSTALLATION AND USE OF THE PRODUCTS OR ANY THIRD PARTY PRODUCT OR SYSTEM. PROSOLIA AND ITS LICENSORS AND THEIR SUPPLIERS

MAKE NO REPRESENTATIONS ABOUT ANY DATA, RESULTS OR INFORMATION PRODUCED OR MADE ACCESSIBLE BY THE PRODUCTS. SOME

JURISDICTIONS DO NOT ALLOW THE EXCLUSION OF IMPLIED WARRANTIES, SO THE ABOVE EXCLUSION MAY NOT APPLY TO CUSTOMER. IN THAT

EVENT, TO THE EXTENT PERMISSIBLE, ANY IMPLIED WARRANTIES ARE LIMITED IN DURATION TO THIRTY (30) DAYS FROM THE DATE OF FIRST

SALE OF THE PRODUCTS.

The foregoing limited warranty shall be qualified by and subject to the standard terms and conditions applicable to the purchase of the Product.

In the event of a claim under the foregoing limited warranty, Prosolia's exclusive liability shall be the repair or replacement, at Prosolia's option,

of any defective systems or parts which Prosolia can confirm are defective and are covered by the warranty. Customer shall be responsible for

any shipping expenses relating to repairs and replacements. All repairs and replacements that are not covered by the limited warranty in Section

3 above are subject to Prosolia’s standard labor and materials charges. Customer must notify Prosolia in writing of any claim regarding shortages,

defective or nonconforming products or parts within 30 days after such defect or nonconformity is or should have been discovered by Customer.

If Customer fails to notify Prosolia timely of a claim, the Products shall be deemed accepted by Customer without objection and any such claim

by Customer shall be waived. Customer is responsible for decontamination of the part(s) or product(s) and providing factual evidence that the

part(s) or product(s) are free from contamination prior to replacement or repair of the defective part(s) or product(s). Customer is responsible

for all such expenses related to decontamination of the part(s) or product(s). If the part(s) or product(s) cannot be made free from contamination,

then the limited warranty is void. Prosolia will not accept under any circumstances, any item that has been exposed to radioactivity or is

microbiologically contaminated. THE REMEDIES PROVIDED TO CUSTOMER HEREIN SHALL BE THE SOLE AND EXCLUSIVE REMEDIES OF CUSTOMER

AND CUSTOMER HEREBY WAIVES ALL OTHER REMEDIES PROVIDED BY APPLICABLE LAW OR EQUITY, INCLUDING WITHOUT LIMITATION, INDIRECT,

INCIDENTAL, CONSEQUENTIAL, LIQUIDATED, PUNITIVE OR ANY OTHER DAMAGES. PROSOLIA SHALL NOT BE LIABLE TO CUSTOMER FOR ANY LOST

PROFITS, ANY INDIRECT, INCIDENTAL, CONSEQUENTIAL, SPECIAL OR SIMILAR DAMAGES (INCLUDING LOSS OF CARGO), OR ANY CLAIMS OR

DEMANDS BROUGHT BY OR AGAINST CUSTOMER, HOWEVER CAUSED AND UNDER ANY THEORY OF LIABILITY. IN NO EVENT SHALL PROSOLIA'S

AGGREGATE LIABILITY TO CUSTOMER ARISING OUT OF, RELATED TO OR IN CONNECTION WITH THE SALE OF ANY PRODUCTS UNDER THIS

AGREEMENT EXCEED THE PURCHASE PRICE PAID TO PROSOLIA BY CUSTOMER FOR SUCH PRODUCTS.

Customer acknowledges that the use of any Third Party Software may be subject to additional end user license agreements (each a "EULA") and

agrees to execute and comply with the terms of any such EULA. EULAs may be contained as a click‐through agreement during the installation of

Third Party Software or in executable form. The use of any Third Party Software shall evidence Customer's acceptance of the terms of the related

EULA. Customer can receive the specific terms of any EULA upon request from Prosolia. Notwithstanding the provisions of Section 4 to the

contrary, Prosolia disclaims any warranty for Third Party Software, and the only warranties that shall apply to Third Party Software shall be those,

if any, which are set forth in the applicable EULA.

iii

Notice of Intended Use

The DESI 2D system should be used for research purposes only and only in an indoor laboratory

environment. The DESI 2D system must not be used in human experimentation or applied to living

humans in any way. The DESI 2D system is not a medical device.

Notice of Proper Use of Prosolia Instruments

If this instrument is used in a manner not specified by Prosolia, the protections provided by the instrument

could be impaired. Any changes or modifications made to the instrument may void compliance with one

or more safety and/or electromagnetic compatibility standards. All replacement parts should be

purchased directly from Prosolia or its authorized representatives. Any necessary repairs should be

performed only by authorized Prosolia personnel or their affiliates.

Notice on Handling and Lifting of Prosolia Instruments

The DESI 2D system is a precision scientific instrument and should be handled with care. While no special

precautions are necessary while transporting the equipment, dropping or other rough handling should be

avoided. If dropped or other rough handling is suspected, please contact Prosolia for further information.

End users should retain all packaging materials for the DESI 2D system in the event the instrument must

be returned to Prosolia for repair. The user is responsible for adequately packaging and the safe transport

of the system to the repair depot.

The DESI 2D system has been designed and is intended to be installed by a single person. For your safety,

DO NOT attempt to lift the DESI 2D system by the cover handles. Only lift the system after removing the

cover and by firmly grasping the sides of the 2D stage or instrument mounting flange. If you are unable

to safely lift 20 lbs (9 kg), obtain the help of a second person before attempting to lift or install the DESI

2D system.

Notice on the Use of Accessory Devices with Prosolia Instruments

The DESI 2D system should only be used with accessories specified and provided by Prosolia and its

authorized representatives. Do not use any other accessory device or consumable components with the

DESI 2D system. Use with unauthorized accessories may void compliance with one or more safety and/or

electromagnetic compatibility standards.

iv

Explanation of Warning Symbols

Electric Shock: This instrument uses high voltages that can cause personal injury. Before servicing, shut down the instrument and disconnect the instrument from line power. Do not use the instrument without its protective enclosure.

Choc électrique: L’instrument utilise des tensions capables d’infliger des blessures corporelles. L’instrument doit être arrêté et débranché de la source de courant avant tout intervention. Ne pas utiliser l'instrument sans son boîtier de protection.

Heat: Surfaces/components present at high temperatures. Allow heated components on the instrument to cool before servicing.

Haute Temperature: Surfaces/composants présents à des températures élevées. Laissez les composants chauds sur l'instrument refroidir avant l'entretien.

Chemical Hazard: This instrument may contain hazardous chemicals. Wear gloves when handling toxic, carcinogenic, mutagenic, or corrosive or irritant chemicals. Use approved containers and proper procedures for disposal of waste. Decontaminate the system before service.

Danger Chimique: Cet instrument peut contenir des produits chimiques. Portez des gants pour manipuler produits chimiques toxiques, cancérigènes, mutagènes, ou corrosifs/irritants. Utiliser des récipients et des procédures appropriés pour l'élimination des déchets. Décontaminez le système avant l’utilisation.

Biohazard: Use of this instrument with biological samples may present a biohazard to the user. Always wear appropriate personal protective equipment when using biohazardous substances and follow your institutions Biological Safety Manual.

Biohazard: Utilisation de cet instrument avec des échantillons biologiques peut présenter un danger biologique à l'utilisateur. Porter toujours un équipement de protection individuelle approprié en utilisant des substances biologiques dangereuses et suivre le manuel de sécurité biologique de votre institution.

v

Eye Hazard: Eye damage could occur from splattered chemicals or flying particles. Wear safety glasses when handling chemicals or servicing the instrument.

Danger pour les yeux: Des projections d’agents chimiques, liquides, ou solides peuvent être dangereuses pour les yeux. Portez des lunettes de protection lors de l’utilisation.

Pinch Hazard: This instrument contains automated, motor driven components. Avoid contact with moving parts.

Pincez danger: Danger de pincement: Cet instrument contient des composants, motorisés automatisés. Eviter le contact avec les pièces mobiles.

General Hazard: A hazard is present that is not included in the above categories. Also, this symbol appears on the instrument to refer the user to instructions in this manual.

Danger général: Indique la présence d’un risque n’appartenant pas aux catégories citées plus haut. Ce symbole figure également sur l’instrument pour renvoyer l’utilisateur aux instructions du présent manuel.

When the safety of a procedure is questionable or unclear, contact Prosolia. Contact

information can be found at www.prosolia.com.

vi

Precautions for Use

The interface components of the mass spectrometer are heated to very high

temperatures. Allow the interface region to cool before installing or removing the

DESI 2D.

Les composants d’interface avec l’appareil de spectrométrie de masse sont

chauffés a très hautes températures. Laissez refroidir les composants d’interface

avant d’installer ou retirer le DESI 2D.

This equipment may be used with substances that present chemical and/or

biological hazards. Wear appropriate personal protective equipment while

operating and servicing the system.

Cet appareil peut être utilise avec de dangereux agents chimiques ou biologiques.

Utilisez des protections individuelles appropriées lors de l’utilisation ou services de

l’appareil.

Systems that are contaminated with biological, carcinogenic, mutagenic,

corrosive, toxic, or irritant hazards must be decontaminated before servicing.

Décontaminez avant utilisation tous les composants contaminés avec des produits

chimiques toxiques, cancérigènes, mutagènes, ou corrosifs/irritants.

To reduce the risk of exposure to hazards, ensure the mass spectrometer is

connected to an exhaust system.

Pour reduire le risque d’exposition, connectez l’appareil de spectrométrie de masse

à un système de d’échappement.

This instrument uses high voltages that can cause personal injury. Before servicing,

shut down the instrument and disconnect the instrument from line power. Do not

use the instrument without its protective enclosure.

L’instrument utilise des tensions capables d’infliger des blessures corporelles.

L’instrument doit être arrêté et débranché de la source de courant avant tout

intervention. Ne pas utiliser l'instrument sans son boîtier de protection.

vii

To maintain compliance with international safety standards, the electrospray high

voltage used with the DESI 2D system must only be supplied from a mass

spectrometer with a power supply whose output does not exceed non‐hazardous

limits established by IEC 61010‐1, clauses 6.3.1 and 6.3.2, as well as the rated input

voltage. Use with higher output currents is unsafe and may lead to electrical shock

or injury.

Pour maintenir la conformité avec les normes internationales de surété, au sujet

de l’électrospray haute tension utilisée avec le système DESI 2D, n’utiliser qu’une

source de courant qui vient d’un spectromètre de masse duquel la sortie

correspond aux limites non‐hasardeuses établies par IEC 61010‐1, clauses 6.3.1 et

6.3.2, aussi bien que la tension indiquée. Si on utilise une source de courant avec

une sortie électrique plus haute, on risque le choc électrique et la blessure.

Moving components are exposed during use and service. Avoid contact with all

moving parts.

Les pièces mobiles sont exposées lors de l’utilisation et du service. Évitez tout

contact avec les pièces mobiles.

Do not use this system in a manner inconsistent with its intended use.

Ne pas utiliser l’appareil d’une manière incompatible avec son utilisation prévue.

Read and understand all instructions before using the system.

Instructions doivent être lues et comprises avant l‘utilisation de l’appareil.

Maintenance and test procedures should only be performed by trained and

qualified individuals.

La maintenance et les tests de procédures doivent être effectués par un personnel

de maintenance qualifié

viii

Safety Considerations

The use of some solvents, reagents, and samples with the DESI 2D system can pose

chemical and biological hazards to the user. Users must know and assess the potential

hazards associated with all substances and samples used with the DESI system. Users

must be trained to evaluate and identify these hazards and in the proper identification,

use, and disposal of personal protective equipment (PPE) used to mitigate these hazards.

Training for chemical and biological hazards and for proper selection and use of PPE is

obtained from your Organization/Institution’s safety representative(s).

This manual contains many safety and special notices. Make sure that you read,

understand, and follow the precautionary statements contained within this manual.

ix

Table of Contents

Limited Warranty .................................................................................................................... ii

Notice of Intended Use ........................................................................................................... iii

Notice of Proper Use of Prosolia Instruments ......................................................................... iii

Notice on Handling and Lifting of Prosolia Instruments .......................................................... iii

Notice on the Use of Accessory Devices with Prosolia Instruments ........................................ iii

Explanation of Warning Symbols ............................................................................................ iv

Precautions for Use ................................................................................................................ vi

Safety Considerations ............................................................................................................ viii

Table of Contents ....................................................................................................................ix

Preface .................................................................................................................................... 1

Contacting Prosolia .................................................................................................................. 2

1. Introduction ........................................................................................................................ 3

1.1 Key References ................................................................................................................................... 4

2. System Components ........................................................................................................... 5

3 DESI 2D System Specifications and Overview ....................................................................... 6

3.1 Specifications ..................................................................................................................................... 6

3.2 DESI 2D System Overview .................................................................................................................. 7

General Operation .................................................................................................................................... 7

System Components ................................................................................................................................. 8

4 DESI 2D System Pre‐Installation Requirements .................................................................. 11

4.1 Line Power ........................................................................................................................................ 11

4.2 Solvents ............................................................................................................................................ 12

4.3 Gases ................................................................................................................................................ 12

4.4 Mass Spectrometer .......................................................................................................................... 13

4.5 Exhaust ............................................................................................................................................. 13

5 DESI 2D System Assembly .................................................................................................. 14

5.1 Camera Installation ........................................................................................................................... 14

5.2 Camera/Lamp Adjustment ................................................................................................................ 16

5.3 Spray Head Assembly ........................................................................................................................ 17

5.4 Gas Connections ................................................................................................................................ 24

5.5 Cable and Tubing Routing ................................................................................................................. 26

6 DESI 2D System Installation ................................................................................................ 27

6.1 Preparation of Waters Xevo Mass Spectrometers ............................................................................ 27

6.2 Mounting the DESI 2D System .......................................................................................................... 28

x

6.3 Ion Transfer Capillary Installation ..................................................................................................... 32

6.4 Installation of the DESI 2D System Cover and Exhaust Line ............................................................. 34

6.5 Removal and Storage of the DESI 2D system .................................................................................... 38

7 Electrical Connections ......................................................................................................... 39

7.1 Electrical Mains Connection .............................................................................................................. 39

7.2 Rear Panel Connections .................................................................................................................... 40

7.3 Front Panel Connections ................................................................................................................... 42

7.4 I/O (Trigger) Cable Connection ......................................................................................................... 43

8 Operation of the DESI 2D System ....................................................................................... 44

8.1 Spray Head Manipulators .................................................................................................................. 44

8.2 Motion of the X and Y Stages ............................................................................................................ 45

8.3 Manual Positioning of the Sample Stage Z Axis ................................................................................ 47

8.4 Spray Head Arm ................................................................................................................................ 47

8.5 High Voltage Interlock ....................................................................................................................... 48

8.6 Slide Holder Operation ...................................................................................................................... 50

8.7 Automating Data Collection with MassLynx™ .................................................................................. 51

9 DESI 2D System Testing ....................................................................................................... 56

9.1 General Operating Parameters ......................................................................................................... 56

9.2 Standards Preparation ...................................................................................................................... 57

9.3 Preparing and Using the Omni Slide for Analysis .............................................................................. 58

9.4 Testing ............................................................................................................................................... 61

9.5 Additional Experimental Examples ................................................................................................... 65

10 Maintenance .................................................................................................................... 70

10.1 General Cleaning ............................................................................................................................. 70

10.2 Preventative Maintenance .............................................................................................................. 71

10.3 Spray Head Nozzle/Emitter Replacement ....................................................................................... 71

10.4 Spray Head Cleaning ....................................................................................................................... 75

10.5 Extended Transfer Capillary Cleaning ............................................................................................. 80

10.6 Fuse Replacement ........................................................................................................................... 81

10.7 Decommissioning and Disposal ....................................................................................................... 82

11 Replacement Parts ............................................................................................................ 83

12 Troubleshooting ............................................................................................................... 86

Hardware Errors ...................................................................................................................................... 86

Communications Errors ........................................................................................................................... 87

Experimental Errors ................................................................................................................................ 87

1

Preface

This manual describes the installation, operation, and maintenance of the DESI 2D system

on Waters Corporation mass spectrometers featuring the Xevo ion source and interface.

This manual contains many safety and special notices. Make sure that you read,

understand, and follow the precautionary statements contained within this manual.

Safety and special notices appearing throughout the manual use the following

conventions:

CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION

notice is accompanied by an appropriate CAUTION symbol.

ATTENTION Le symbole CAUTION/ATTENTION indique une situation potentiellement

dangereuse pour l’utilisateur, les biens ou environnent. Chaque CAUTION/ ATTENTION

sont accompagnés d’un symbole d’alerte pour reconnaitre la nature du danger

potentiel.

IMPORTANT Highlights information necessary for proper operation of the system.

NOTE Highlights general information of interest to the user.

TIP Highlights helpful information that can make a task easier to perform.

2

Contacting Prosolia

There are several ways to reach Prosolia. Our standard business hours are from 8 AM to

5 PM Eastern Standard Time, Monday through Friday.

General Inquires/Ordering Information

Phone 866‐241‐0239 or 317‐275‐5794

Fax 317‐873‐3175

E‐mail [email protected]

Web www.prosolia.com

Technical Support

Phone 866‐241‐0239 or 317‐275‐5794

Fax 317‐873‐3175

E‐mail [email protected]

Web www.prosolia.com

3

1. Introduction

The DESI 2D system has been designed to give you the full advantage of the Desorption

Electrospray Ionization (DESI) technology originally invented in the laboratory of Prof. R.

Graham Cooks at Purdue University. This manual contains the installation and operating

instructions for the DESI 2D System with automated sample positioning that is compatible

with the Waters mass spectrometers with the Xevo atmospheric pressure interface. The

DESI 2D system is simple to operate. It eliminates the need for complex and time

consuming sample preparation allowing for faster results.

Desorption Electrospray Ionization (DESI) technology* was first reported by Prof. Cooks’

group in Science (2004, 306, 471‐473). DESI is a pneumatically assisted electrospray

technique that directs an appropriate solvent mixture towards the sample of interest and

the resultant ions are sampled directly into the mass analyzer, as shown below, all under

ambient conditions. The contents of the solvent spray, the gas flow rate, the amount of

applied voltage, the spray angle and the ion uptake angle, as well as the various distances

in aligning the spray, sample and mass spectrometer are all variables which can be studied

to achieve an optimal mass spectrum for a particular type of sample.

ILLUSTRATION OF DESORPTION ELECTROSPRAY IONIZATION

DESI has been demonstrated in a wide range of applications from the detection of

explosives to proteomics. References for reviews on this exciting technology are listed

below. An expanded publications list related to DESI is available on our website,

www.prosolia.com

We hope you enjoy the DESI 2D experience.

*US Patent 7,335,897

freely moving sample stage

spray emitter

sprayspray nozzle

inlet of mass spectrometer

sample

desorbed ions

solvent N2

H

4

1.1 Key References

Takats, Z.; Wiseman, J. M.; Gologan, B.; Cooks, R. G., Mass Spectrometry Sampling Under

Ambient Conditions with Desorption Electrospray Ionization. Science 2004, 306, 471‐473.

Takats, Z.; Wiseman, J. M.; Cooks, R. G., Ambient Mass Spectrometry using Desorption

Electrospray Ionization (DESI): Instrumentation, Mechanisms and Applications in

Forensics, Chemistry, and Biology. J. Mass Spectrom. 2005, 40, (10), 1261‐1275.

Cooks, R. G.; Ouyang, Z.; Takats, Z.; Wiseman, J. M.; Ambient Mass Spectrometry. Science

2006, 311, 1566‐1570.

Omni Spray Ion Source 2‐D Automation Software Installation and Reference Manual

5

2. System Components

The DESI 2D system is shipped to the user packaged in several boxes, the contents of

which will vary based on the compatible mass spectrometer. Each system is configured

with the following components:

DESI 2D system compatible with Waters mass spectrometers

DESI 2D system installation and accessory kit

DESI spray head kit

DESI 2D electronics kit

Region specific mains power cord

Mass spectrometer I/O cable

Instruction manua

6

3 DESI 2D System Specifications and Overview

The DESI 2D system has been designed to be operated in a normal laboratory

environment. Details of the specifications can be found in later sections of this manual.

For software specifications, please refer to the Omni Spray Ion Source 2D Automation

Software Installation and Reference Manual.

3.1 Specifications

Physical

DESI 2D System

Size ‐ H 218mm (8.6”) x W 358.5mm (14.1”) x D 279.5mm (11.0”)

Weight – Approximately 8 kg (17.6 lbs)

Control Electronics

Size ‐ H 181mm (7.2”) x W 184mm (7.25”) x D 58mm (2.3”)

Weight – Approximately 1.5 kg (3.3 lbs)

Environmental

Temperature – 5‐40°C (41 ‐ 104 °F), Optimum Operating 15‐30°C (59 ‐ 86°F).

Relative Humidity – 20‐80% with no condensation

Altitude – Sea level to 3000m

Environment – Indoor laboratory environment, no direct sunlight

Electrical

DESI 2D System – 24 VDC ±10%, 3A Max

AC/DC Power Supply – Input: 100‐240 VAC ±10%, ~2A , 50/60 Hz, Output: 24VDC, 2.5A, 60W Spray HV input* – ±8kVDC , 100 µA Max

7

*CAUTION To maintain compliance with international safety standards, the

electrospray high voltage used with the DESI 2D system must only be supplied from

a mass spectrometer with a power supply whose output does not exceed non‐

hazardous limits established by IEC/EN/UL 61010‐1, clauses 6.3.1 and 6.3.2, as well

as the rated input voltage. Use with higher output currents is unsafe and may lead

to electrical shock or injury.

ATTENTION Pour maintenir la conformité avec les normes internationales de surété,

au sujet de l’électrospray haute tension utilisée avec le système DESI 2D, n’utiliser

qu’une source de courant qui vient d’un spectromètre de masse duquel la sortie

correspond aux limites non‐hasardeuses établies par IEC/EN/UL 61010‐1, clauses

6.3.1 et 6.3.2, aussi bien que la tension indiquée. Si on utilise une source de courant

avec une sortie électrique plus haute, on risque le choc électrique et la blessure.

3.2 DESI 2D System Overview

The following section provides an overview of the system to acquaint the user with the

features of the DESI 2D system for Waters Xevo mass spectrometers.

General Operation

The DESI 2D system is an automated surface sampling and ionization source for mass

spectrometry featuring desorption electrospray ionization technology. In DESI, ions are

generated from a sample situated on a surface by way of bombardment with high

velocity, charged micro‐droplets. The spray impact at the surface causes the formation

of microscopic liquid layers on the sample surface into which analyte dissolve. Desorption

of micron‐sized droplets containing dissolved analyte occurs via momentum transfer

when additional droplets from the spray collide with the liquid layer forcing the desorbed

droplets into the gas phase. These droplets are sampled by the sampling aperture of the

mass spectrometer for analysis.

The DESI 2D system is controlled using Prosolia’s Omni Spray 2D Motion Control Software,

operating on the mass spectrometer’s data system computer. The software

communicates to the DESI control electronics though USB and is responsible for the

control of the 2D sample stage in manual and automated movement modes. Automated

sample analysis experiments, such as surface imaging, are programmed into the motion

control software along with the appropriate sample sequence in the mass spectrometer

software. The DESI 2D system triggers data acquisition on the mass spectrometer in

synchronization with the progress of the DESI experiment.

8

System Components

The following figures describe the location of major components of the OS‐6205 DESI 2D

system which are referenced throughout this manual. In these figures, wiring and tubing

has been removed for clarity.

Instrument Mounting Flange

Each DESI 2D system is designed to closely couple to compatible mass spectrometers

through as integrated mounting flange which supports the 2D stage, positioning

manipulators for the DESI spray head, a camera for visualizing the sprayer/inlet region,

and a safety enclosure to prevent access to hazards during operation. Figure 3.2.1 shows

the instrument mounting flange and three (3) mounting fasteners. Instruction for

mounting the DESI 2D system to the mass spectrometer are found in Section 6 of this

manual.

Figure 3.2.1 Instrument mounting flange showing the three (3) mounting fasteners

2D Sample Stage

The 2D sample stage is automated for positioning sample plates relative to the inlet of

the mass spectrometer for analysis. Mounted to the 2D stage is a sample plate holder,

capable of carrying one (1) microtiter footprint slide or two (2) 76 x 26 mm glass

microscope slides. The stage features and over all travel of 120 x 80 mm and performs

several automated scan types through the Omni Spray 2D Motion Control Software.

Details on the operation of the stage are found in the Motion Control software manual

and in Section 8.2.

9

Figure3.2.2 2D Sample stage and sample plate holder

Spray Head Manipulator

The DESI spray head is mounted into the manipulator assembly allowing for the spray tip

to be positioned in three axes and rotationally relative to the sample surface and mass

spectrometer inlet. Figure 3.3.3 shows the rotational manipulator assembly and Section

8.1 describes its use.

Figure 3.3.3 Illustration of the spray head rotational/linear manipulators for adjusting the spray emitter position.

10

System Enclosure

The DESI 2D system for Waters Xevo mass spectrometers includes a system cover to

prevent access to the system components will in operation and the help shield users from

solvent vapors and sample aerosols when connected to a proper exhaust system. Details

of the enclosure are given in Sections 6.4 and 8.5.

Figure 3.2.4 OS‐6205 DESI 2D system with the system enclosure in place

11

4 DESI 2D System Pre‐Installation Requirements

The DESI 2D system has minimal requirements for operation and use. This section details

these requirements which must be met before installation can begin:

4.1 Line Power

The DESI 2D system includes an AC/DC power supply with a wide ranging input that will

operate worldwide. The DESI 2D system requires one (1) outlet near the mass

spectrometer for operation. The line voltage should be free from fluctuations, surges,

sags, and transients in voltage

Mains Power: 100‐240 VAC, ~2A, 50/60 Hz

When installing the equipment, position the unit such that the unit can easily be

unplugged from the mains voltage (outlet) in an emergency.

CAUTION For safety compliance, the outlet must be connected to Earth ground.

ATTENTION Conformément aux mesures de sécurité générales, la prise électrique doit

être connectée et mise a la terre.

CAUTION Use only the original power supply provided with the system.

ATTENTION L’appareil doit uniquement être utilisé a partir d’une source d’énergie qui remplitles spécifications mentionnées sur l’étiquette de puissance électrique du produit

CAUTION Do not replace the mains supply cord with one of inadequate rating.

ATTENTION Ne remplacez pas les cordes électriques avec un matériel non agrée et de

différentes caractéristiques.

12

Country Plug Type Diagram

USA/Canada NEMA 5‐15P

United Kingdom BS 1363

Europe CEE 7/7

Australia AS 3112

Switzerland SEV 1011

China GB 2099

Note: Authorized distributors may supply additional power cord types as required

Table 4.1.1 List of mains power cords supplied with the DESI 2D System

4.2 Solvents

The DESI 2D system requires the use of HPLC grade solvents and modifiers for best

performance. Solvents and reagents used should be free from impurities.

Solvents are delivered to the DESI 2D System at flow rates between 1 and 5 L/min. The

solvent delivery system (typically a syringe pump) used with the DESI 2D system should

be free from pulsation at the above flow rates. If you do not have a suitable solvent

delivery pump, recommended syringe pumps can be purchased directly from Prosolia.

Please contact for more information.

4.3 Gases

A high pressure nitrogen gas source is required for the operation of the DESI 2D System.

This nitrogen gas is used to aid in the formation of the DESI spray and direct the droplets

produced towards the sample surface. A regulated, high pressure nitrogen source must

be provided with a variable pressure between 80‐150 PSI (5.5‐10.3 bar), and preferably

variably between 80‐200 PSI (5.5‐13.8 bar). The nitrogen gas should be free from

contamination and at a recommended purity of 99.999%.

The DESI 2D System features a ¼ inch, Swagelok® compression fitting for the nebulizing

gas input. Due to the varying length of tubing necessary for each installation, the tubing

has not been provided with the ion source. Recommended tubing for this connection is

13

¼ inch outer diameter PFA tubing (1/8th inch inner diameter), available from suppliers

such as Swagelok, Upchurch Scientific, McMaster‐Carr, and others. Ensure that the ¼ inch

tubing used to make the connection between your high pressure nitrogen source and the

DESI 2D System is rated to a working pressure of at least 250 PSI.

4.4 Mass Spectrometer

The DESI 2D system is designed and engineered to be compatible with certain models of

mass spectrometer. The components of the DESI 2D system are provided for specific

mass spectrometers based on customer requirements. The DESI 2D system shall only be

used on systems which are deemed compatible by Prosolia. Use on non‐compatible mass

spectrometers is not supported and may present the user with safety hazards not

anticipated in the design and engineering of the system.

Compatible mass spectrometers should be installed, tuned, and calibrated according to

the manufacturer’s instructions for best performance. Any required ventilation for the

mass spectrometer vacuum pumps and ionization source must be installed according to

the mass spectrometer manufacturer’s requirements. The instruments shall be in good

repair and free from defects or modifications. Use on mass spectrometers that have been

modified is not supported and may present the user with safety hazards not anticipated

in the design and engineering of the system.

4.5 Exhaust

The DESI 2D system is an atmospheric pressure ionization source which creates an aerosol

of spray solvents and samples during analysis Although the volumes of solvent and

sample consumed during this process is small, there is the potential for aerosol/vapors to

escape and thus the users must be aware of the potential chemical hazards. Each

laboratory and user MUST assess the hazards and risks associated with the spray solvents

and samples in use in their laboratory environment during use and employ appropriate

PPE if hazards warrant its use.

The DESI 2D system is designed to be connected to a local exhaust ventilation system

during operation. The user must provide a proper, negative pressure exhaust system with

a minimum vacuum of 5 mbar below atmospheric pressure. Connection to the exhaust

system is detailed in Section 6.4

14

5 DESI 2D System Assembly

Prior to the installation of your new DESI 2D system onto your mass spectrometer, some

simple assembly is required. All of the tools necessary for the assembly of the DESI system

have been included with the system kits. The directions in this section will acquaint users

with the DESI 2D system and explain how to assemble the system for use. Items not

included in the kit, which are necessary for the operation of the ion source, include a high

pressure nitrogen source that can be regulated between 80‐200 psig and high purity

solvents. The requirements for these items are discussed in following sections of this

manual.

Figure 5.1 DESI 2D system kit for the Waters mass spectrometers with the Xevo interface. (Note: all parts not shown)

5.1 Camera Installation

The DESI 2D system is equipped with a single video camera and LED lamp to assist in the

positioning and monitoring of the spray and surface. Both the camera and LED lamp are

attached to the spray head arm. The camera assembly is located in the electronics

accessory kit and will not be attached to the ion source when shipped. The LED light will

be in place on the system when shipped from Prosolia.

15

Figure 5.1.1 DESI 2D system camera clamp and LED light locations

The camera is equipped with a mirror to capture images at a 90˚ angle to the body of the

camera. Follow the steps below to install the camera and as shown in Figure 5.2.2

1. Open up the camera clamp by loosening the clamp screw.

2. Insert the camera from the back of the clamp loop with the mirror of the camera entering

the clamp first. You may need to gently pull on the upper portion of the camera clamp to

allow the camera body to slide freely.

3. Turn the camera housing until the mirror is pointing down towards the surface.

4. Tighten the clamp screw to secure the camera in place.

NOTE To obtain a video picture with the proper orientation, it may be necessary to

make small adjustments to the mirror rotation relative to the camera body. To make

this adjustment, loosen or tighten the threaded lens assembly slightly.

Camera Clamp

LED Light

16

Figure 5.1.2 Installation of the camera to view the sample surface

5.2 Camera/Lamp Adjustment

The camera and LED light will need to be adjusted once the system is mounted and

running on the mass spectrometer. Changes in camera position is made by loosening the

clamp screw, moving the camera horizontally within the clamp, and retightening the

clamp screw. The camera should be adjusted to look straight down towards the sample

surface so that the spray head tip, capillary inlet, and sample surface can be seen within

the same image. Focus on the camera is achieved by adjusting the camera height relative

to the surface. The camera height is changed my turning the adjustment knob as shown

in Figure 5.2.1. The focal length of the cameras is approximately 7 cm. The LED lamp can

also be adjusted by pivoting the lamp using the adjustment lever.

Tighten Screw

Insert Camera

17

Figure 5.2.1 Location of the camera adjustment knob on the spray head arm

5.3 Spray Head Assembly

The spray head of the DESI 2D system comes preloaded with a fused silica emitter. Before

using the system, the length of the emitter which protrudes from the nozzle must be

adjusted by turning the nozzle clockwise using the 4 mm wrench provided with your

component kit (see section 10.3 for further details). The emitter should protrude

approximately 1mm beyond the tip of the spray nozzle.

Figure 5.3.1 Adjustment of the emitter protrusion using a 4 mm wrench

Connections for both the spray solvent and nitrogen nebulizing gas must be made to the

spray head. The tubing and fittings necessary to make these connections have been

provided with your DESI 2D system.

Camera Adjustment Knob

Light Adjustment Lever

18

Figure 5.3.2 Connections made to the DESI spray head

Figure 5.3.3 Exploded view of DESI spray head

Spray head solvent connection:

1. Remove Microtight nut and ferrule from the rear port of the spray head.

2. Insert yellow Microtight sleeve (P300‐00004‐01) through the ferrule so that it protrudes

approximately 4 mm from the point of the ferrule

3. Slide nut over the provided fused silica tubing (part number P300‐00011‐99) followed by

carefully inserting the fused silica through the sleeve

4. Using a capillary cleaving tool, cut ~1 cm of the fused silica tubing to ensure the end is cut

square and free of debris

5. Pull the fused silica back through the sleeve such that the ends are flush

6. Insert the ferrule/sleeve/fused silica into the port of the spray head tee. They should

bottom out in the tee and automatically set the depth within the tee

7. Tighten nut over threads to secure the connection

The liquid path through the spray head includes a grounded union to prevent the high

voltage applied to the spray head from reaching the syringe. The stainless steel union

which makes the liquid junction for the ground point is attached to the system via a holder

under the 2‐D platform. The union can be snapped in place by placing it at the opening

of the holder and pushing into the slot. The union will be held in place when seated

completely into the holder. To remove, simply push the union downwards from the

holder.

Solvent connection

Neubulizer gas connection

HV connection

19

CAUTION To avoid a possible static like shock, always place the grounding union into

the holder. Do not handle the grounding union while the instrument is in Operate

Mode.

ATTENTION Pour éviter un choc statique semblable, placez toujours l'union de la terre

dans le support. Ne manipulez pas l'union de la terre lorsque l'appareil est en Mode

d'exploitation.

Figure 5.3.4 Location of grounding union under the system’s 2‐D platform

The free end of the fused silica capillary is connected to the grounding union. To make

this connection:

1. Insert a 1/16th inch PEEK ferrule (P300‐00017‐02) into a red fingertight nut

(P300‐00016‐01)

2. Insert 1/16th inch yellow sleeve (P300‐00018‐01) through the nut/ferrule so that it

protrudes approximately 4 mm from the point of the ferrule

3. Using a capillary cleaving tool, cut the fused silica tubing to length. Ensure the tubing will

reach the grounding union holder and will have sufficient length to allow the spray head

and manipulator to move through their entire range of motion

4. Carefully insert the end of the fused silica solvent tubing through the 1/16th inch yellow

sleeve

5. Again, using a capillary cleaving tool, cut ~1 cm of the fused silica tubing to ensure the

end is cut square and free of debris

6. Pull the fused silica back through the sleeve such that the ends are flush

7. Insert this assembly into an open end of the stainless steel union (P300‐00015‐01) and

tighten firmly

Grounding Union Holder

Gas Manifold and Shut-off Valve

20

The connection between the grounding union and the syringe is made using 1/32nd inch

PEEK tubing for ruggedness. The first connection to make with the 1/32nd inch PEEK

tubing is to the grounding union.

1. Insert a 1/32nd inch PEEK ferrule (P300‐00029‐01) into a red finger tight nut

2. Insert one end of the 1/32nd inch PEEK tubing (P300‐00047‐01) through the nut/ferrule so

that it protrudes approximately 4 mm from the point of the ferrule

3. Insert this assembly into an open end of the stainless steel union and tighten firmly

Figure 5.3.5 Syringe adapter connections for solvent delivery to the DESI spray head

Finally, the opposite end of the 1/32nd inch PEEK tubing is connected to the syringe though

the adapter union. To make this connection:

1. Insert the gauge plug provided with the adapter union (P300‐00030‐01) into the union

and gently tighten into place

2. Cut the PEEK tubing to the appropriate length to reach the syringe pump

3. Carefully insert the end of the 1/32nd inch PEEK through the smaller threaded fitting so

that it protrudes approximately 4 mm from the tip

4. Insert this assembly into the open end of the adapter union and tighten firmly

5. Remove the gauge plug from the larger end of the adapter union

6. Insert the short length of Teflon tubing supplied with the system (P300‐00013‐01) into

the larger threaded fitting leaving approximately 4 mm protruding from the tip

7. Insert this assembly into the open end of the adapter union and tighten until snug

8. Insert the needle of the syringe into the Teflon tube. The fitting may need to be loosened

and then retighten to permit the needle to pass through the sealing area

SS union adapter

Ferrules 1/32” PEEK tubing

Fused Silica

21

Figure 5.3.6 Syringe adapter connections for solvent delivery to the DESI spray head

For details on making the gas connection, please see section 5.4.

With the gas and liquid connection made, the spray head can be placed in the rotational

manipulator of the DESI 2D system.

1. Remove cap from rotational manipulator by removing the two hex head screws (see

Figure 5.3.7)

2. Place the spray head into the retaining cap so that the high voltage connector points

through the hole on the face of the cap as shown in Figure 5.3.8. Push the cap onto the

spray head until the high voltage cap is protrudes through the hole and the spray head

sits flat against the cap. The gas connection should be located on below the retaining cap

and the solvent connection will fit in the grove of the cap

3. Place the spray head and cap into the open end of the rotational manipulator with the

spray head pointing down

4. While holding the spray head and cap in position, evenly tighten the screws to secure the

spray head in the manipulator (Figure 5.3.8)

Union adapter

Gauge plug

1/32” PEEK Tubing

Teflon sleeve

22

Figure 5.3.7 Rotational manipulator before the spray head is installed

Figure 5.3.8 Rotational manipulator with the spray head is installed

At this time, the red high voltage cable can also be connected to the red liquid junction

connector of the spray head. To connect, insert the 2 mm banana plug into the jack at

the end of the high voltage wire.

The emitter installed in the spray head will need to be replaced if it becomes clogged or

damaged. Refer to the Section 10.3 for instructions for emitter replacement. Damage is

Retaining Cap

HV Connector

23

often caused by crashing the spray head into the inlet of the mass spectrometer or the

sample surface. Use caution when positioning the spray head to avoid this damage.

To change the angle at which the spray head is positioned, turn the rotational knob to the

desired angle as shown in Figure 5.3.9. The manipulator is capable of providing a spray

head angle anywhere between 40 – 80 degrees. The actual spray head angle is indicated

by the mark that is just visible on the rotational arm as it exits the holder.

Figure 5.3.9 Spray head angle adjustment

Figure 5.3.10 Spray head X‐Y‐Z position adjustment

Finally, the spray head arm has an X‐Y‐Z manual manipulator as shown in Figure 5.3.10.

This three axis manipulator is used to position the spray head relative to the sample and

capillary inlet. See Section 9 for a detailed discussion of the optimal angle and position

settings of the spray head.

Angle Indicator

Adjustment Knob

Y Axis, Z Axis, X Axis

24

5.4 Gas Connections

A high pressure nitrogen gas source is required for operation of the DESI 2D system. This

nitrogen gas is used to aid in the formation of the DESI spray and direct the droplets

produced towards the sample surface. Tefzel tubing (P300‐00010‐03) has been provided

with the system kits to make the gas connecting lines between 1) the gas manifold and 2)

the gas shutoff valve mounted under system’s the 2‐D stage (Figure 5.4.1). Another

section of tubing is necessary to make the gas connection between 1) the gas shutoff

valve and 2) the spray head gas connection (Figure 5.4.2)

Using the supplied length of tubing, make the first tubing connections as follows:

1. Cut the end of the provided tubing square using an appropriate tubing cutter or razor

blade

2. Slide the red Vacutight nut and ferrule onto the cut end of the tubing

3. Insert the Vacutight fitting and tubing into the threaded port on gas manifold mounted at

the ends of the drawer guide rails and tighten appropriately

4. Connect the other end of the tubing to the shutoff valve, cutting the tubing to length.

5. Slide the nut and ferrule from the shutoff valve onto the tubing

6. Insert the fittings and tubing into the port of the valve and tighten appropriately

Using the remaining tubing, make the second tubing connection between the shutoff

valve and the spray head as follows:

1. Ensure the end of the tubing has been cut square. If not, cut the end of the tubing square

using an appropriate tubing cutter or razor blade

2. Slide the nut and ferrule from the shutoff valve onto the cut end of the tubing

3. Insert the fittings and tubing into the port of the valve and tighten appropriately

4. Connect the other end of the tubing to the spray head, cutting the tubing to length.

5. Slide the red Vacutight nut and ferrule onto the cut end of the tubing

6. Insert the Vacutight fitting and tubing into the threaded port on the spray head and

tighten appropriately

NOTE When using the DESI 2D cover assembly, the gas line tubing must be cut with

sufficient length to be routed through the opening at the front of the cover and to the

spray head. See section 6.4 for details on using the cover with the DESI 2D system.

The nitrogen gas input connection to the DESI 2D system is made at the gas manifold at

the end of the drawer guide rails using a ¼ inch Swagelok fitting. Prosolia does not supply

this tubing as the necessary length will vary according to the location of the gas supply in

your laboratory. Ensure that the ¼ inch tubing used to make the connection between

your high pressure nitrogen source and the DESI 2D system is rated to a working pressure

of at least 250 PSI.

25

Figure 5.4.2 Gas connections on the DESI 2D system

Gas Connection #1Gas Manifold and Shut-off Valve

Gas Connection #2

26

5.5 Cable and Tubing Routing

The DESI 2D system features cable and tubing routing locations on the spray head arm

and ion source flange to help keep wires and tubing neat and out of the way. Routing

cables and tubing through these locations also keeps cables out of the way when using

the system cover. Figure 5.5.1 shows these routing locations. When using the routing

points, be sure to have enough slack in the connections to operate the spray head arm

through its full range of motion.

Figure 5.5.1 DESI 2D system cable and tube routing locations, circled

27

6 DESI 2D System Installation

The initial installation of the DESI 2D system may be performed by the end user, Prosolia,

or its authorized representatives. This manual details installation and removal, the user

controls, operation, and safety aspects of the DESI 2D system for normal, daily use. The

manual also details maintenance procedures and spare/replaceable components of the

system

All tools necessary to install and maintain the DESI 2D system have been included in the

system’s accessory kit. In addition to the components included with the system,

operation of the system requires a compatible mass spectrometer, a source of high purity

nitrogen gas, and common LC‐MS grade solvents and modifiers.

The DESI 2D System has been designed to attach to the mass spectrometer in a similar

manner to the instrument’s original electrospray ionization source, with minimal

modification to the mass spectrometer. This section of the manual will acquaint the user

with the installation procedure for the DESI 2D system. Users should fully read and

understand the entire manual before beginning the installing and using the system on

their mass spectrometer.

6.1 Preparation of Waters Xevo Mass Spectrometers

Prior to the installation of the DESI 2D system onto a Waters mass spectrometer, the

existing ionization source must be removed. Remove the original ionization source from

the mass spectrometer according to the procedure described in the mass spectrometer

hardware manual. Ensure all gas and liquid flows have been shut off, the lines are

depressurized, and the interface temperature has cooled before removing the ionization

source.

CAUTION The mass spectrometer interface components are hot. Reduce the

temperature of the interface and allow to cool before removing the ionization source.

ATTENTION Les composants de l’interface de l’appareil de spectrométrie de masse sont

chauds. Laissez les composants refroidir avant de retirer la source d’ionisation.

1. Ensure the instrument has been placed in Standby mode.

2. With the ionization source removed and the interface cooled to room temperature,

close vacuum isolation valve.

3. Remove the ion sampling cone/cone gas nozzle by rotating the components clockwise

to release them from the ion block.

4. Carefully remove the ion sampling cone/cone gas nozzle. Use caution to avoid

dropping the ion sampling cone

5. Remove the ion sampling cone and o‐ring for use with the bazooka inlet tube.

28

Figure 6.1.1 shows the Waters Synapt G2 mass spectrometer with the ion source removed

and the removal of ion sampling cone.

CAUTION Do not install the extended Bazooka inlet tube at this time. The DESI 2D

source must be installed prior to installation of the inlet tube.

ATTENTION Ne pas installer le tube d'entrée Bazooka étendu à ce moment. La source

DESI 2D doit être installé avant l'installation du tube d'entrée.

Figure 6.1.1 Z‐Spray interface of the Synapt G2 with ion sampling cone installed (left) and removed (right)

6.2 Mounting the DESI 2D System

After preparing the mass spectrometer, installation of the DESI 2D system can proceed.

Installation requires the use of a 5 mm hex key which should be located for use prior to

beginning installation.

Installation of the Latch Mounting Block

1. Remove the retaining pin from the latch mounting block by completely unscrewing from

the mounting block.

2. Place the latch mounting block over the source latch hook on the right hand side of the

Xevo interface with the ‘foot’ pointing towards the ion block (see Figure 6.2.1).

29

Figure 6.2.1 Xevo interface flange with latch mounting block and gas plugs installed. Note the orientation of the latch mounting block.

3. Insert the retaining pin into the latch mounting block and tighten completely.

4. Insert a gas plug (P800‐00271‐01) into each gas port on the upper left side of the Xevo

interface (see Figure 6.2.1). The plugs should fit snugly into the port.

a. On some systems, the gas plugs may fit tightly into the port. A twisting action

may be required to inset and remove the plug.

b. Certain systems require the use of a larger o‐ring (3x1mm, part number

P100‐00088‐01 ). If the plug fits loosely into the port, exchange the o‐ring for the

3x1mm o‐ring provided with the kit.

5. Replace o‐rings when they show signs of wear.

TIP Choose the gas plug o‐ring that best fits your mass spectrometer. The fit should

be snug.

Small – 2.5 x 1 mm – Part Number P100‐00086‐01

Large – 3 x 1 mm – Part Number P100‐00088‐01

Latch Mounting Block

Gas Plugs

30

Installation of the DESI 2D System

The system is now prepared for the installation of the DESI 2D system hardware. If you

are unable to safely lift 20 pounds (9 kg), obtain the help of a second person for this step

of the installation process.

1. If installed, remove the DESI system cover and set aside.

2. Prepare the DESI 2D system for mounting by sliding the source interlock slider towards

the back of the system flange (see Figure 6.2.2) and loosening the mounting screws on

the left hand side of the flange. Do not completely remove these screws.

Figure 6.2.2 Interlock slider in the proper rearward position for mounting the DESI system to the mass spectrometer

CAUTION Do not attempt to install the ion source with the extended capillary in place.

Doing so may lead to damage to the capillary and the potential for electrical shock.

ATTENTION Ne pas essayer d'installer la source d'ions avec le capillaire prolongé en

place. Cela peut entraîner des dommages au capillaire et le potentiel de choc

électrique.

3. Holding onto the sides of the 2D stage (see Figure 6.2.3), carefully mount the DESI 2D

system by placing the system over the mounting pins of the Xevo interface and sliding

downwards to seat the system on the interface. This procedure is done in a similar

manner as mounting the standard Xevo ion source.

4. Insert the right hand mounting screw (located near the spray head hinge) through the

flange and into the latch mounting block. It may be necessary to adjust the system

position slightly to align the holes.

5. Tighten all mounting screws (three) using the 5 mm hex key to secure the DESI 2D system

to the mass spectrometer.

6. Push the source interlock slide towards the instrument to close the source interlock

switch (Figure 6.2.2). This slider satisfies only one of the interlock requirements for the

instrument. See section 8.5 for details.

31

Figure 6.2.3 Mounting the DESI 2D system to the mass spectrometer by sliding the system over the mounting pins of the Xevo interface.

Once the DESI 2D system has been mounted onto the mass spectrometer, additional

connections for gas and electronics can be made.

1. The source interlock cable is plugged into the Blue socket under the sliding door, located

directly above the ion source region. This connection is keyed to be inserted in one

orientation only.

2. The high voltage cable is then plugged into the High Voltage socket located above the

upper left corner of the ion source.

3. Finally, the nitrogen and other electrical connections (cameras, LED Lamp, etc.) to the

system can be made (see Section 7).

32

Figure 6.2.4 Location of the source interlock and high voltage cable connections

6.3 Ion Transfer Capillary Installation

Efficient collection of the ions generated in the DESI 2D system requires a close proximity

of the ion inlet of the mass spectrometer to the sample being analyzed. The standard ion

sampling cone used on the Waters Z‐Spray interface restricts one to analyzing only a very

small sample without the use of an inlet extension capillary. A large diameter, bazooka

ion transfer capillary has been included with the system to allow for analysis of larger

sample areas with Waters mass spectrometers. In addition, the ion transfer capillary is

slightly inclined such that there is clearance for samples to pass below the capillary.

To install the bazooka capillary ion inlet, the ion sampling cone and the cone gas nozzle

must have first been removed from the instrument following the instructions given in

Section 6.1.

IMPORTANT The installation of the extended ion transfer tube MUST be performed

after mounting the DESI 2D system to the mass spectrometer.

1. Carefully assemble the bazooka extension by placing the ion sampling cone into the

bazooka fitting in the same manner in which it fits the original desolvation gas cone.

2. Place the o‐ring around the base of the ion sampling cone as in Figure 6.3.2.

33

Figure 6.3.1 Bazooka extension components showing proper orientation of parts

Figure 6.3.2 Bazooka extension with ion sampling cone and o‐ring installed

3. Slide the ion transfer capillary assembly into receiving slot on the ion block with the inlet

tubing pointing straight upwards. The ion sampling cone will insert into the ion block.

This part fits the same manner as the ion sampling cone/cone gas nozzle.

4. Carefully place the 5 mm wrench provided with the system kit over the wrench flats on

the bazooka inlet cone.

5. Rotate the bazooka inlet counter clockwise (downwards) until the front edge of the

bazooka cone is aligned with the front edge of the ion block. The capillary will have a slight

(5°) downward slope when installed.

6. Slowly turn the handle of the vacuum isolation valve to its open position.

7. Return ion block temperature to its normal operating value.

34

Figure 6.3.3 Finished installation of the ion transfer capillary.

6.4 Installation of the DESI 2D System Cover and Exhaust Line

The DESI 2D system for Waters Xevo mass spectrometers includes a system cover to

prevent access to the source components will in operation and the help shield users from

solvent vapors and sample aerosols when connected to a proper exhaust system. The

use of the system cover is required. The lid of the cover is interlocked and must be closed

for the instrument to operate. For more details, please see Section 8.5.

CAUTION The cover and exhaust line connection are provided to reduce, but will not

eliminate exposure to any solvent or sample aerosols created by DESI 2D system.

Additional personal protective measures may be necessary to eliminate operator

exposure to aerosols created by the source.

ATTENTION La connexion de la ligne d'échappement et la couverture sont prévus pour

réduire, mais pas éliminer l'exposition à des aérosols ou de solvants échantillons créés

par la source d'ions DESI 2D. Mesures de protection personnelle supplémentaires

peuvent être nécessaires pour éliminer l'exposition de l'opérateur aux aérosols créés

par la source.

35

Exhaust Line Connection

The exhaust line connection, shown in Figure 6.4.1 below, is located on the bottom of the

source platform. An external exhaust or vacuum system should be attached to this

connection to assist in capturing aerosols generated by the DESI 2D system. A ¾” or 1”

hose can be attached to the vent line connector.

Users MUST perform a hazard risk assessment to evaluate the need for additional

personal protective equipment when operating the DESI 2D system. The use of varied

sample types and varied spray solvents can lead to hazards not anticipated in the design

of the product. Consult your organizations Environmental, Health, and Safety (EHS)

personnel and Prosolia for more information regarding these hazards.

Figure 6.4.1 Location of DESI 2D system vent fitting.

Installation of the System Cover

The DESI 2D system cover is connected to the instrument mounting flange via two pins

located on the top surface of the flange as shown in Figure 6.4.2. To install the cover:

1. Move the 2D stage to the middle of its travel in both directions.

2. Holding the cover by the side handles, carefully place the cover onto the mounting

pins using care to avoid pinching cables or tubing. Lift straight upwards and avoid

tipping the cover as it is removed.

TIP Due to the close fit between the cover and the mounting pins, the cover must be

lifted straight upwards to avoid binding on the mounting pins.

36

Figure 6.4.2 Location of system cover retaining pins

NOTE It may be necessary to move the sample stage position prior to

installing/removing the system cover. The cover cannot be installed /removed with

the stage at the home position.

When properly installed, a gap is left between the cover and the system flange for passage

of the spray head solvent and gas supply lines as well as the camera and LED cables. Care

should be taken when installing or removing the cover not to pinch or damage these lines

and cables.

Operation of the Cover Lid

The system cover features a hinged lid for easy access to the system components. The lid

is opened using the cover on the front of the cover as shown in Figure 6.4.3. When in the

opened position, the cover lock can be moved into a locked position to prevent the lid

from closing unexpectedly. To lock and unlock the lid, simply push the locking mechanism

in the desired direction.

37

Figure 6.4.3 Handle for opening of the system lid (left) and location of the cover locking slide (right)

Opening the lid will trip the mass spectrometer interlock, disabling the electrospray and

cone voltages. The lid must be in the closed position to operate the mass spectrometer.

Figure 6.4.4 Photograph showing the DESI 2D system and cover installed on a Waters Synapt G2 HDMS mass spectrometer

Cover Lock

38

6.5 Removal and Storage of the DESI 2D system

Removal of the DESI 2D system from the mass spectrometer is the reverse of the

installation procedure. Before removing the system, allow the interface of the mass

spectrometer to cool.

1. Reduce the interface temperature of the mass spectrometer and place it in Standby

mode.

2. Turn off the power to the DESI control electronics.

3. Turn off the nitrogen gas to the DESI 2D system and allow the gas lines to

depressurize.

4. Remove the DESI 2D cover and set aside.

5. Remove the syringe pump connection from the syringe needle and place the union

on top of the 2D stage.

6. Unplug the LED light and Camera connections from the from the DESI control

electronics.

7. Unscrew and remove the 25 pin motor control cable from the bottom of the DESI 2D

stage.

8. Remove the exhaust line if connected.

9. Unplug the Interlock and high voltage connections from the mass spectrometer.

10. Close the vacuum isolation valve on the mass spectrometer and remove the extended

ion transfer tube.

11. Slide the source interlock slider away from the mass spectrometer (see Figure 6.2.2).

12. Completely remove the mounting screw on the right hand side of the flange.

13. Loosen the left hand mounting screws and slide the system upwards on the face on

the mass spectrometer to remove.

14. Remove the latch mounting block. Reinstall the retaining pin and right hand

mounting screw to prevent these components from becoming lost.

15. Remove the gas plugs from the mass spectrometer interface by pulling and twisting.

The DESI 2D system is intended to be stored on a shelf or table top in the upright position,

sitting the lower portion of the instrument mounting flange and the bottom components

of the system. The cover can be placed on the system while in storage to prevent damage

to the DESI spray head.

CAUTION Do not lift the DESI 2D system by the handles of the source cover. The cover

is not attached to the source and it will separate and fall, damaging the system and

potentially causing personal injury.

ATTENTION Ne pas soulever la source DESI 2D par les poignées du couvercle de la

source. Le couvercle est pas attaché à la source et il va se séparer et tomber,

endommager le système et qui pourrait causer des blessures.

39

7 Electrical Connections

Electrical connections for control of the automated sample stage of the DESI 2D system,

power to the camera, LED lamp, USB communication to the instrument data system, and

the I/O cable for triggering of the mass spectrometer are made at the DESI 2D control

box. The following section details the electrical connections to the control electronics

and mass spectrometer.

Figure 7.1.1 Photograph of the DESI 2D motion control box.

7.1 Electrical Mains Connection

The DESI 2D system requires one wall outlet to be available at the mass spectrometer for

system power. The mains power for the system shall meet the requirements outlined in

Section 4.1. Plug the mains power cord into the power supply before connecting to line

power. In the case of an emergency, the mains power cord serves as the disconnecting

device and should be removed from the wall outlet to remove the mains power from the

system.

CAUTION When installing the equipment, position the unit such that the power cord

can easily be unplugged from the mains voltage (outlet) in an emergency.

ATTENTION Assurez que l’interrupteur général ainsi que le connecteur pour le câble

électrique soient facilement accessibles et qu’ils se trouvent le plus proche possible de

l’operateur de l’appareil.

40

CAUTION Place the DESI control box and power supply away from areas where

flammable liquids are likely to be spilled. Spilling flammable liquids onto the control

box or power supply may result in a fire.

ATTENTION Place la boîte de commande DESI et bloc d'alimentation loin des zones où

des liquides inflammables sont susceptibles d'être déversé. Tout déversement de

liquides inflammables sur la boîte de commande DESI ou bloc d'alimentation peut

provoquer un incendie.

The DESI control box is designed to be operated at a nominal voltage of either 120V or

230V AC, 50/60 Hz. Your system will be shipped complete with the appropriate power

cord as listed in Section 4.1

7.2 Rear Panel Connections

The rear panel of the DESI control box contains the connections for the control electronics

power, the USB communication port, and video connection from the camera. In addition,

the 1 ampere protection fuse is also located on the rear panel of the control electronics.

Figure 7.2.1 Electrical connections made on rear panel of the DESI control electronics box

Communications Connection:

1. Insert B‐type plug end of the supplied USB communications cable into the ‘COMPUTER’

connection on the rear panel.

2. Insert A‐type plug end of the supplied USB communications into any USB port on the back

of the computer (Figure 7.2.2).

USB Fuse DC Power Video

41

NOTE The USB connection to the instrument data systems should be made directly to

a USB port on the data system computer, not through a USB expansion hub (such as

those common on monitors). Failure to connect directly to the computer may result

in communication errors between the computer and control electronics.

Figure 7.2.2 Typical serial port location on rear I/O panel of computer

Video Capture Card / Camera Connection

The video image from the camera is displayed within the DESI control software window

on your computer monitor. This requires the installation of the ViewCast Osprey 210

video capture card and drivers provided with your system. To install the video capture

card, consult your IT Support personnel and follow the manufacturer’s installation

instructions provided with the video card.

Camera Connection to the DESI Control Electronics

To connect the video camera to the video capture card, begin by connecting one end of

the supplied BNC‐BNC video cable to the VIDEO 1 OUT connector located on the rear

panel of the DESI control electronics. The opposite end of the cable will be connected to

the video capture card as described below.

Camera Connection to the Video Capture Card

1. Install the video capture card and drivers in the computer per the manufacturer’s

instructions. An installation manual is included on the CD with the video capture card.

NOTE When installing the video capture card software, select NTSC as the video

format.

2. Attach the breakout cable supplied with the video capture card to the 15 pin D‐sub

connector on the video capture card.

3. Connect the free end of the supplied video cable to the yellow BNC jack of the video

capture card’s breakout cable

USB Ports

42

7.3 Front Panel Connections

The front panel of the DESI control box contains the connections for the video camera,

the LED light source, the trigger cable to the mass spectrometer, and the DESI 2D stage.

In addition, the front panel also contains a power status lamp built into the power switch.

Some connections at the front panel are made using locking Lemo connectors, which are

keyed to ensure the proper orientation of the plug. To make connections at the front

panel, simply hold the connector with the red dot facing up and insert it into the proper

socket on the front panel. A ‘click’ will be heard when the connector has been locked into

place. To remove the plugs, simply pull back on the locking sleeve to unlatch and remove

the plug.

Figure 7.3.1 Insertion (left) and removal(right) of the Lemo connections on the front panel of the DESI control electronics box.

VIDEO 1 IN – The supplied video camera should be connected to the control electronics

though this video input connection

LIGHT – This connection provides power to the LED light source.

I/O – This connection is used to trigger the mass spectrometer for automated data

collection. The end of the supplied cable with the Lemo connector should be plugged into

this socket. The other end of the cable should be plugged into the I/O port of the mass

spectrometer (see section 7.4).

MOTOR CONTROL – This connection provides power to the motors which drive the

motion of the sample stage in the both the X and Y directions.

A 25 pin, D‐subminiature cable has been supplied to connect the control box to the 2D

stage for control of the X and Y axis stages. Each end of the cable locks to the mating

fitting by thumbscrews.

43

7.4 I/O (Trigger) Cable Connection

The DESI System control electronics are capable of triggering the mass spectrometer to

collect data from multiple samples much like a standard autosampler in LC‐MS. The

connection to the proper port on the mass spectrometer must be made to use this

function. A cable with the proper termination for the instrument specified at the time of

order has been included with the source.

With the five pin Lemo connector plugged into the I/O port on the front of the control

box, plug the opposite end of the cable into the I/O port of the mass spectrometer. To

make this connection, you may need to unplug any connection from an LC system that

may be plugged into the I/O port of the mass spectrometer.

CAUTION To avoid electric shock, all electrical connections to the I/O connector must

be separated from hazardous voltages by double or reinforced insulation. Circuits of

this type are classified as safety extra low voltage (SLEV).

ATTENTION Pour éviter les chocs électriques, toutes les connexions électriques pour

le connecteur d'e/s doivent être séparées de tensions dangereuses par une isolation

double ou renforcée. Circuits de ce type sont qualifiés de safety extra low voltage

(SLEV).

Pin Pin ID I/O Cable Color Description

1 Start + White Trigger signal – Do not apply external voltage to

pin

5 Ground Black Digital Ground

Table 7.4.1 Pin out of the I/O port and cable.

44

8 Operation of the DESI 2D System

DESI 2D Systems are simple to operate. This section details the operation of the system

including positioning of the spray head arm, operation of the sample stage Z‐axis,

movement of the manual manipulators, operation of the automated X and Y stages,

insertion/removal of samples, and source interlock functions. Prior to operating the

system, the DESI control software must be installed on the mass spectrometer’s data

system computer and the electronics connected. See the Omni Spray Ion Source 2‐D

Automation Software Installation and Reference Manual for further information on

software installation.

8.1 Spray Head Manipulators

Proper positioning of the spray head is critical to maximizing the performance of the DESI

2D system. Not only can the position of the spray head change in the X, Y, and Z axis, its

angle is also variable.

To change the angle at which the spray head is positioned, turn the rotational knob to the

desired angle as shown in Figure 8.1.1. The manipulator is capable of providing a spray

head angle anywhere between 40 – 80 degrees. The actual spray head angle is indicated

by the mark that is just visible on the rotational arm as it exits the holder.

Figure 8.1.1 Spray head angle adjustments

The spray head arm also has an X‐Y‐Z manual manipulator as shown in Figure 8.1.2. This

three axis manipulator is used to position the spray head relative to the sample and

capillary inlet. See Section 9 for a detailed discussion of the optimal angle and position

settings of the spray head.

Angle Indicator

Adjustment Knob

45

Each axis on the three axis manipulator has a locking screw. There is a lock symbol next

to each screw and an arrow that indicates the direction to lock down the manipulator. It

is recommended that these manipulators are locked once the spray head position has

been set. However, it is important to loosen these screws before adjusting the

manipulator. Failure to loosen the locking screw will result in damage to the manipulator.

Figure 8.1.2 Spray head X‐Y‐Z position adjustment

Figure 8.1.3 Manipulator locking screws

8.2 Motion of the X and Y Stages

The X‐direction and Y‐direction of the sample positioning has been automated to provide

computer controlled movement. Control of the sample position is achieved using the

Omni Spray 2‐D Control Software. The X and Y stages can be positioned manually within

the software or through several automated motion profiles for analysis of multiple

Y Axis, Z Axis, X Axis

Z Axis Locking Screw

46

samples. Please see the Omni Spray Ion Source 2‐D Automation Software Installation and

Reference Manual for further information on the types of motion available.

In general, there are two operating modes that can be used to position the sample by

moving the X and Y stages which are described below.

Manual Motion Control

Operation under manual motion control allows for the free movement of the sample

surface. In this mode of operation, commonly used for investigating new samples, source

optimization, etc., continuous data collection is the preferred method for saving results.

Movement of the stages is driven by dragging of the position indicator within the software

window.

Automated Motion Control

Under automated motion control, the automated DESI 2D system can reproducibly collect

data from individual samples, much like an autosampler of a typical LC/MS system.

Typically, three steps are necessary to perform an automated sequence analysis.

1. Construction of the motion sequence within the Omni Spray control software ‐ The

sample analysis sequence is constructed in the DESI software under the desired

automated motion profile.

2. Development of the mass spectrometer analysis method ‐ Using the calculated

acquisition time from the automated motion sequence, mass spectrometer methods are

developed using the appropriate data analysis time.

3. Construction of sample sequence in mass spectrometer data system – A sample analysis

sequence is created in the mass spectrometer data system to match the number of

samples in the Omni Spray control software using the methods developed in step 2.

Following the creation of both a sample sequence within the mass spectrometers data

system and the motion sequence within the Omni Spray control software, the sample

plate can be analyzed.

1. The DESI 2D system must be in the proper operating position (optimized positions

previously determined), with the sample placed in the sample holder.

2. The sample sequence is started within the mass spectrometer’s data system. Users must

wait until the mass spectrometer is ready to receive a start trigger from the DESI 2D

system before preceding the step 3.

3. The motion sequence is started within the Omni Spray control software. The mass

spectrometer will trigger automatically and begin analysis of the sample.

47

8.3 Manual Positioning of the Sample Stage Z Axis

Unlike the movement of the X and Y axis, the Z axis of the sample stage can only be

adjusted by manually. The location of the Z axis adjustment knob is shown in Figure 5.3.1.

By turning the adjustment screw, the entire X and Y stage is raised or lowered allowing

for different sized samples to be analyzed. When raising the sample stage, use caution to

avoid crashing the spray head into the sample and possibly damaging the spray

nozzle/emitter.

Figure 8.3.1 Location of the Z axis adjustment screw near the 2D stage

8.4 Spray Head Arm

The spray head arm on the DESI 2D system pivots near the ion source flange to allow for

access to the sample surface for easy sample changing. The arm is locked into the

operating position using a cam/lever mechanism. To rotate the spray head from its

operating position, turn the handle on top of the hinge approximately ¼ turn clockwise,

as shown in Figure 8.4.1. The handle will point to the Z axis adjustment knob when the

hinge is released. With the hinge released, simply push the spray head arm to the right

to rotate it out of position.

To put the spray head arm back into operating position, simply return the spray hard arm

to position and turn the locking handle counter clockwise to secure. Turning the handle

will lock the hinge mechanism and pull it into alignment with the ion source flange.

Z Axis Adjustment

48

Figure 8.4.1 Rotate the locking handle (left) ¼ turn clockwise to release the locking mechanism on the hinge

Figure 8.4.2 Spray head arm rotated to allow access to the sample surface

8.5 High Voltage Interlock

The DESI 2D System maintains the safety interlock features of the mass spectrometer such

that when the system is taken out of its operating position (removed from the mass

spectrometer or the enclosure lid is opened), the high voltage from the mass

spectrometer is disabled. For Waters Xevo mass spectrometers, there are two interlock

requirements that must be met for the system to operate properly. The first interlock

switch that must be closed is located within the mounting flange of the mass

spectrometer. This interlock requirement is met using a manually actuated mechanism

when the system is installed onto the mass spectrometer. The interlock slider (see Figure

6.2.2), located at the top of the interface flange MUST be pushed fully forward after the

system has been installed for the mass spectrometer to operate.

49

This model of the DESI 2D system features an interlocked system enclosure to prevent

user access to high voltage components of the system and mass spectrometer during

operation. This enclosure must be in place with the lid closed for the instrument to

operate. Both interlock requirements must be met for the mass spectrometer to operate

properly.

CAUTION To avoid a possible static like shock, do not touch the mass spectrometer

interface components or the spray head while the instrument is in Operate Mode.

ATTENTION Pour éviter un choc statique semblable, ne touchez pas les composants de

l'interface du spectromètre de masse ou la tête de pulvérisation alors que l'instrument

est en Operate Mode.

To allow for adjustment of the spray head position and the sample stage height while the

instrument is operating, the system enclosure features tool access holes. To make these

adjustments, hex drivers with insulating handles have been provided with the DESI 2D

system kit. To make the adjustments, simply insert the proper insulated handle hex driver

through the proper opening in the system enclosure and into the hex socket of the

manipulator handle. Use caution when adjusting the spray head manipulator. If excess

resistance is felt, stop and ensure the locking screws are not tightened to prevent damage

to the manipulator.

CAUTION Use the insulated hex drivers provided to make position adjustments while

the instrument is operating. NEVER insert other tools or objects into the adjustment

holes due to risk of electrical shock.

Figure 8.5.1 Adjusting the sample stage height (left) and Y‐axis of the spray head manipulator using the insulated hex drivers

50

8.6 Slide Holder Operation

The sample holder of the DESI 2D system is designed to hold up to two standard 76 x 26

mm microscope slides or one slide fitting the microtiter plate standard footprint (127.76

mm X 85.48 mm). The each location in that can accept a slide in the sample holder has a

spring clip which is used to secure the sample slide(s) in the holder and prevent vibrations

during automated motion.

To insert a sample into the slide holder, pull the spring clip back while placing the slide in

place. Release the spring clip to secure the slide (see Figure 8.6.1). Slide(s) already in the

holder can be removed by the finger groove provided. Always be careful not to touch the

sample area while installing or removing the slide(s).

Periodically, dust and debris will need to be removed from the slide holder to allow

surfaces to sit flat on the holder. The slide holder may be cleaned with 70% isopropyl

alcohol to remove contamination. A gentile stream of nitrogen or clean compressed air

can be used to blow dust from the slide holder surface. Care should be taken to avoid

blowing this dust and debris onto the precision guide rail or motor lead screws of the 2D

automated stage.

Figure 8.6.1 Sample holder showing spring clips and locations for 76 x 26 mm slides

Spring Clip

Sample Holders for 76 x 26 mm slides

51

8.7 Automating Data Collection with MassLynx™

To utilize automated data collection using MassLynx and your DESI 2D system, you must

first ensure that the instrument is configured properly to accept the contact closure signal

through MassLynx. The contact closure type is through the ‘Inlet Method’ editor as

described below.

1. Select the Inlet Method editor from the left hand column under the ‘Instrument’ tab in

the MassLynx window

2. Select ‘Instrument Configuration…’ from the Tools menu

3. Remove any configured devices (LC Systems, detectors, etc.) by selecting the ‘Configure’

button and selecting ‘None’ for each component. See Figure 8.7.1

4. Configure the instrument to accept the contact closure input by selecting the

‘Events & Triggering’ button

5. Check the box next to ‘Event In 1’ to enable the instrument to accept the contact closure.

See Figure 8.7.2

Figure 8.7.1 Screen shot of Inlet Method window showing configuration for the DESI 2D System

52

Figure 8.7.2 Screen shot of Inlet Method window showing configuration of the instrument to accept a contact closure

Creating an MS method in MassLynx with your DESI 2D system

In this section, important considerations when creating a MS method to be used with your

DESI 2D system are discussed.

TIP In most cases, the instrument should be properly tuned in MS Tune prior to

executing a sample sequence. Parameters (voltages, syringe pump flow rate, etc.) set

in the MS Tune program will be used in the MS method for analysis

1. Select the MS Method editor from the left hand column under the ‘Instrument’ tab in the

MassLynx window

2. Edit the MS method to include the desired MS experiments. It is suggested to use voltages

from the MS Tune page

The total acquisition time should be set in accordance with the calculated sample

acquisition time for your experiments in the DESI control software. See Figure 8.7.3.

NOTE The DESI 2D system has a 5 second post‐acquisition delay by default. If the

acquisition time in MassLynx is set too long relative to the calculated acquisition time

established in the DESI 2D motion system, data acquisition steps will not be

synchronized.

53

Figure 8.7.3 MS Method editor in MassLynx. Note total run time circled in figure must match the calculated acquisition time in the DESI software to maintain synchronization between MassLynx and the DESI 2D system

Creating a sample sequence in MassLynx for automated data acquisition

Please follow the instructions detailed in the MassLynx software manual for information

on how to set‐up a sample sequence. Your MassLynx sequence should match the motion

sequence in the DESI control software in the number of samples to be run.

1. Add samples to the sample queue in the MassLynx window

2. For each sample, choose the appropriate MS Method

3. An inlet method is not necessary if the instrument has been configured as described

above. The MS Status display will show a contact closure is configured See Figure 8.7.4

Once you have set‐up a sample sequence in Mass Lynx, follow these steps to execute the

sequence.

1. Select ‘Start from the ‘Run’ toolbar in MassLynx.

2. In the Start Sample List Run window, enter the row numbers to analyze and click ‘OK’

See Figure 5.7.4

NOTE The number of rows of the sample sequence must match the number of rows in

the motion sequence in the Omni Spray control software to maintain synchronization

between the mass spectrometer and the DESI 2D System.

54

3. The instrument status located in the lower left of the MassLynx window should now read

“Waiting for inlet start 1” See Figure 8.7.5

4. Return to the DESI automation software, click ‘Start’ to begin sample analysis.

TIP Follow the steps outlined in Section 6.4 Sample Spot Calibration prior to executing

the sample sequence.

NOTE If the data acquisition system does not begin collecting data (i.e. it is still waiting

on the contact closure), ensure that your DESI 2D hardware is properly installed. Refer

to Section 7 of this manual for connection information.

Figure 8.7.4 Screen shot of the MassLynx ‘Start Sample List Run’ window

55

Figure 8.7.5 Screen shot of the MassLynx window indicating the mass spectrometer is waiting for the contact closure signal.

56

9 DESI 2D System Testing

The procedures and instructions in this section of the manual are intended to help new

users become familiar with performing DESI experiments on the DESI 2D system and to

guide them through the initial testing of the system. If problems arise with the operation

of the system, performance testing using the following procedures is suggested.

9.1 General Operating Parameters

When operating your DESI 2D system there are many degrees of freedom that when

changed will vary the geometry of the experiment. These parameters include the spray

impact angle (α), the tip to surface distance (d1), the tip to inlet distance (d2), and the

surface to inlet distance (d3). Recommended operating parameters are displayed below

in the table Typical Operating Parameters. Figure 9.1.1 below shows the relative location

of the sample, spray tip, and instrument inlet using the suggested operating parameters

given in the table. In general, the spray head angle can be positioned between 40‐90

degrees, with the tip at a distance of 1‐5 mm from the sample surface. The distance from

the sample to the instrument inlet is around 2‐5 mm. As mentioned previously, the

nitrogen pressure for the nebulizing gas is varied between 80‐200 PSIG. For each type of

sample and for different solvent compositions, these variables may need to be adjusted

to get the optimal results.

Figure 9.1.1 Relative Position of the Sample, Spray Head, and Instrument

57

Parameter Setting

Electrospray voltage 2.75 kV

Solvent flow rate 2.0 L/min

Gas pressure 100 PSI

Tip to surface distance (d1) 2 mm

Tip to inlet distance (d2) 3‐4 mm

Surface to inlet distance (d3) ~0.5mm

Spray impact angle () 55°

MS inlet temperature 150 °C

Table 9.1.1 Typical operating parameters

9.2 Standards Preparation

Dried chemical standards have been included with the DESI 2D system to be used for

initial testing and familiarization with the system. The following reagents will be

necessary to reconstitute the dried standards:

Acetonitrile (HPLC Grade)

Water (HPLC Grade)

Formic acid (HPLC Grade)

Preparation of bradykinin Peptide Standard

Reconstitute the bradykinin peptide standard (MW: 1060.1) (part # P900‐00307‐01) provided in your DESI 2D system kit by pipetting 1.00 mL of 80:20 acetonitrile:water directly into the glass vial to give a 1 mg/mL stock solution of bradykinin. Vortex or shake to ensure standard is dissolved.

Prepare a working standard solution of bradykinin at10 µg/mL by pipetting 10.0 µL of the stock solution into the provided glass vial. Add 990.0 µL of 80:20 acetonitrile:water and vortex or shake to ensure mixing.

58

9.3 Preparing and Using the Omni Slide for Analysis

Omni Slide™ Sample Surfaces are disposable sample surfaces for use with Prosolia’s DESI

systems. Omni Slide sample surfaces are designed to aid the user in executing

reproducible experiments from a standardized surface. They are available in a variety of

sizes and formats to meet different experimental needs. This section describes the use

of two formats on Omni Slide Hydrophobic Arrays. The Omni Slide Hydrophobic Array

substrates allow for reproducible sample spotting and improved signal stability in high‐

throughput and quantitative studies. These arrays also provide discrete sample spotting

locations and position references which can be easily programmed into the Omni Spray

control software.

Omni Slide Micro‐24 Sample Arrays – The Omni Slide Micro‐24 Sample Array substrates

(shown in Figure 9.3.1) are a76 x 26 mm microscope glass slide with a printed 24 spot

array. The slides have sample locations of 3 mm diameter and a center‐to‐center

separation of 4.00 mm. The large white surface at the end of the slide is writable and

intended to be the end handled by the user.

Figure 9.3.1 Layout of 24 well Omni Slide Micro‐24 Array

MT‐96 Sample Arrays – The Omni Slide MT‐96 Sample Array substrates (shown in Figure

9.3.2) are a microtiter format glass slide with a printed 96 spot array. The slides have

sample locations of 3 mm diameter and a center‐to‐center separation of 9.00 mm. The

sample location pattern meets the microtiter format standard. The white bar at the

bottom of the slide is writable for noting sample information.

4.003 mm dia.

4.00

4.00

4.00

59

Figure 9.3.2 Layout of 96 well Omni Slide MT‐96 hydrophobic array

Sample Deposition Direction ‐ The automated motion sequences performed by the DESI

2D system begins from a neutral point in the margin area. As such, when a sequence is

started, the sample stage will first move to the neutral point, followed by approaching

the first sample from the left. In general, samples are analyzed from left to right, front to

back. When possible, as a matter of good practice, samples should be placed in an order

in which the lowest concentration samples are analyzed first, followed by samples of

increasing concentration, to prevent the possible contamination of the mass

spectrometer inlet.

Use of the Origin Feature – The origin feature is commonly used to create a relative origin

from which sample positions are measured. For example, when using an MT‐96 Omni

Slide sample substrate, samples are spotted in an array at regular intervals. The Omni

Slide features and alignment cross such that the spray can be aligned with the surface and

an origin can be applied to take into account the manufacturing variance of sample spot

locations on the glass substrate. This method removes the need to modify sample

positions for variance in the spot location if the origin is set to align each surface placed

in the sample holder using the same reference point on the sample slide.

9.00 3 mm dia.

9.00

5.50

5.50

60

Align sample surface to a

reference point such as the

center of the alignment cross or

the center of a sample spot.

Set the origin at this point Program the location of the

samples into the Omni Spray 2‐D

control software

When placing next sample slide in

holder:

Using same reference point,

align the next sample slide

Set the offset origin at this point to adjust for differences in the

spot location relative to the first

slide

The previous sample locations

entered will now be valid for

this sample slide

Figure 9.3.3 Layout of 96 well Omni Slide MT‐96 hydrophobic array

Set origin location

Align inlet/spray to cross

Samples at regular intervals

61

9.4 Testing

Prior to beginning your experiments using the DESI 2D system, the mass spectrometer

should be calibrated and tuned for best sensitivity for the type(s) of compound(s) of

interest (i.e. small molecules, proteins, etc.), and free of contamination in the ion

interface/optics. All solvents used with your system should be HPLC grade and free from

contamination/impurities. Use caution when preparing solutions to avoid the

introduction of contaminants such as plasticizers. It is recommended that users follow

the procedure outline below in order to familiarize one with operating the DESI system.

It is recommended that users become familiar with the given references about DESI to

learn the implications of varying the parameters of the experiment. The example

experiments given in this section are performed using the geometric parameters

described in Section 9.1 and use 50:50 acetonitrile:water + 0.1% formic acid (v/v) as the

spray solvent.

NOTE The data presented in section 6.4 represents typical data collected on Waters

Synapt G2 for the standards supplied with the DESI system. Results on other

instruments will vary based on the age and condition of the instrument, the level of

contamination in the interface region, instrument tuning, etc., as well as the exact

model of instrument onto which the system is installed .

Omni Slide Preparation (76 x 26 mm)

1. Mark the center of the first spot (furthest spot to the left) on an Omni Slide using the red

Sharpie provided to you in your DESI 2D system kit. Refer to Figure 9.3.1.

2. Deposit 1µL of bradykinin peptide standard working solution onto the center of the

second, third, and forth spots on the Omni Slide.

NOTE Be sure to pipette consistently across the row on the Omni Slide as too much

deviation in droplet location will significantly affect your results.

3. Allow all spots to dry completely prior to analysis.

Omni Slide Preparation (Microtiter Plate Format)

1. Mark the center of the cross located at the top right hand corner of an Omni Slide

(position 0,0) using the red Sharpie provided to you in your DESI 2D system kit. Refer to

Figure 9.3.2

2. Deposit 1µL of bradykinin peptide standard working solution onto the center of spots A1,

A2, and A3 on the Omni Slide.

62

Sample Spot Calibration

Follow the procedure below to align the spray head using the DESI 2D motion system with

the row where you deposited the standard solutions.

1. Turn‐on the power to the DESI motion control power supply.

2. Double click Omni Spray 2‐Dicon from the start menu. The motion system should

initialize, execute a ‘calibration’ routine, and return to home.

NOTE Please refer to the Omni Spray Ion Source 2‐D Automation Software Installation

and Reference Manual for instructions on loading the Omni Spray 2‐D automation

software.

3. Using the Omni Slide prepared in the previous section place the surface in the slide holder

of your DESI 2D system according to the orientation suggested above.

4. Turn on the spray solvent delivery system (typically a syringe pump) and nitrogen gas flow

to your DESI 2D system. These examples use 50:50 acetonitrile:water: 0.1% formic acid

(v/v) as the spray solvent. Allow the flows time to stabilize.

5. Turn on the mass spectrometer.

TIP Wait approximately 1 minute for the spray to stabilize.

6. With the motion system set at the default origin (2, 2 mm), establish geometrical

parameters according to the suggested operating parameters in Section 9.1.

7. Using the Omni Spray 2‐D Motion Control software, move the surface to the location

where the red dye was deposited using the manual motion control buttons (see Figure

9.4.1).

Figure 9.4.1 Manual positioning parameters in the Omni Spray control software window

Goal Position

Current Stage Position

Current Origin Location

Set Origin Button Return to Origin Button

Manual Motion Control

63

1. Optimize the signal intensity of the ion at mass/charge (m/z) 443 by finely adjusting the

spray head to inlet distance (d2). An example mass spectrum of the red dye is shown in

Figure 6.4.2 below.

2. Set the origin at this location using the Set Origin button and proceed to sample analysis.

NOTE Data presented in this section is from a single scan of the MS, choosen at the

peak intensity of the ion of interest. Combining spectra, as is typically done, will

improve both ion intensity and in most cases S/N.

Figure 9.4.2 DESI mass spectrum of the red Sharpie marker on the Omni Slide hydrophobic surface. The ion at mass/charge 443 corresponds to the intact cation of rhodamine 6G.

NOTE This example shows typical results when the procedure above is followed.

If you do not observe the signal at 443, ensure that:

The syringe pump is ‘ON’ and the spray solvent is flowing without obstruction.

The capillary emitter is protruding out of the nozzle according to the

recommendations in Section 2.4.

All operating parameters are similar to the recommendations in Section 6.3.

Sharpie - 50/50 ACN/H2O w/ 0.1% FA

m/z100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000

%

0

100

DESI_STD_RG6_04 3 (0.068) TOF MS ES+ 2.81e4443.3006

28130

149.04714767

301.19121687

444.30367981

445.30781822

64

Sample analysis

The DESI 2D system gives users the option of several different motion profiles, as detailed

in the Omni Spray Ion Source 2‐D Automation Software Installation and Reference

Manual. Additionally, the Omni Spray 2‐D automation software is set‐up to sync with the

instrument’s data acquisition system using the contact closure feature. This feature

allows automated data acquisition on a spot by spot basis, if desired. Please refer to

Sections 3 and 4 of this manual for details about the installation of the DESI motion control

hardware and connecting the contact closure cable.

Bradykinin peptide standard – The analysis of bradykinin peptide standard deposited

onto an Omni Slide should produce signal intensities comparable to that shown in Figure

9.4.2. Multiply‐charged ions corresponding to the triply‐, doubly, and singly‐protonated

forms of bradykinin will be present. Additionally, some adduct formation with sodium

and/or potassium ions may also be present, as indicated in Figure 9.4.2.

Figure 9.4.2 DESI mass spectrum (centroid) of bradykinin (10ng/spot)

m/z100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 1000

%

0

100

DESI_STD_BKTEST_05 54 (0.939) TOF MS ES+ 2.11e3530.8535

2111

531.36691052

[M+2H]2+

65

9.5 Additional Experimental Examples

The following section shows additional experimental examples utilizing your

automated DESI 2D system. Note that the data presented here was not collected using

a Waters mass spectrometer, but is intended to serve as a demonstration of the

automated capabilities of the DESI system.

Point to Point – Dwell Typical results when using the point‐to‐point dwell mode (a typical

setup is shown in Figure 9.5.1) for consecutive 10ng deposits of bradykinin peptide

standard are shown in Figure 9.5.2. The selected ion current for bradykinin at m/z

530.9±1 is displayed for three consecutive spots. The data from each spot was collected

in one data file. The integrated peak areas show an inter‐spot variability of approximately

10% RSD.

Figure 9.5.1 Example 96 sample point to point – dwell motion profile

66

Figure 9.5.2 Selected ion current of 10ng bradykinin [M+2H]2+ in triplicate using a point to point – dwell

Point to Point – Oscillate The oscillate motion profile allows you to move the surface

beneath the spray head at defined velocities and intervals. This feature is advantageous

when locating a ‘sweet spot’ within the sample analysis area and also enables averaging

over a larger surface area than in the point to point – dwell mode. The results of a typical

point to point – oscillate experiment (a typical setup is shown in Figure 9.5.3) for

consecutive 10ng deposits of bradykinin peptide standard are shown in Figure 9.5.4. The

selected ion current for bradykinin at m/z 530.9±1 is displayed for three consecutive

spots. The data from each spot was collected in one data file.

RT: 0.00 - 0.67 SM: 7G

0.00 0.05 0.10 0.15 0.20 0.25 0.30 0.35 0.40 0.45 0.50 0.55 0.60 0.65Time (min)

0

5

10

15

20

25

30

35

40

45

50

55

60

65

70

75

80

85

90

95

100

Rel

ativ

e A

bu

nda

nce

RT: 0.05MA: 705977 RT: 0.64

MA: 663343

RT: 0.32MA: 597726

0.510.50 0.520.09 0.12

0.14

0.300.35

0.360.150.19

0.620.56 0.590.39

0.41

0.01

NL:9.96E4m/z= 528.77-533.08 MS bradykinin_ptopdwell_04

67

Figure 9.5.3 Example 66 sample point to point‐oscillate motion profile

Figure 9.5.4 Selected ion current of 10ng bradykinin [M+2H]2+ in triplicate using the point to point oscillate motion profile shown in Figure 9.5.3

Point to point – constant velocity (CV) The point to point – CV motion profile allows you

to move the surface beneath the spray head at defined velocities and intervals. This

feature is advantageous as it allows for rapid data collection and when scanning across

RT: 0.00 - 1.36 SM: 7G

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 1.1 1.2 1.3Time (min)

0

5

10

15

20

25

30

35

40

45

50

55

60

65

70

75

80

85

90

95

100

Re

lativ

e A

bun

da

nce

RT: 0.62MA: 391154

RT: 0.12MA: 354851

RT: 1.03MA: 347060

0.590.550.17

0.88

1.050.710.98

0.66 1.271.180.330.27 1.080.840.800.460.21 0.75 1.10 1.24

0.350.96 1.13

0.37 0.390.40

0.95

1.340.03

NL:2.47E4m/z= 530.35-531.35 MS bradykinin_cv_osc_03

68

deposited samples, reproducible peak shapes for integration. The results of a typical

point to point – CV experiment (a typical setup is shown in Figure 9.5.5) for consecutive

10ng deposits of bradykinin peptide standard are shown in Figure 9.5.6. The selected ion

current for bradykinin at m/z 530.9±1 is displayed for an experiment with four

consecutive spots. The data from all spot was collected in one data file.

Figure 9.5.5 Example 96 sample point to point – constant velocity motion profile

69

Figure 9.5.6 Selected ion current of 10ng bradykinin [M+2H]2+ in quadruplicate using a point to point continuous velocity scan

RT: 0.00 - 2.00 SM: 7G

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9

Tim e (m in)

0

5

10

15

20

25

30

35

40

45

50

55

60

65

70

75

80

85

90

95

100

Re

lativ

e A

bu

nd

an

ce

RT: 1.08AA: 615484

RT: 1.58AA: 630854

RT: 0.60AA: 570844

RT: 0.10AA: 394590

NL:1.58E5

m /z= 530.38-531.38 MS ICIS bk_ptopcv_05

70

10 Maintenance

The DESI 2D system requires little maintenance during normal use. During the initial

system installation, system users will be trained in the proper maintenance and test

procedures. This section details the routing cleaning and maintenance needs of the DESI

system, including the replacement of the spray nozzle and spray head emitter. To

minimize the risk of injury these procedures should be performed only by qualified and

trained individuals knowledgeable of the risks of both the maintenance procedures and

any samples/chemicals used with the system.

CAUTION Maintenance and test procedures should only be performed by trained and

qualified individuals.

ATTENTION La maintenance et les tests de procédures doivent être effectués par un

personnel de maintenance qualifié

10.1 General Cleaning

During operation, the surfaces of the DESI 2D system may become soiled or

contaminated. The system should be disconnected from the mass spectrometer and from

power before cleaning. All surfaces of the DESI system may be wiped with a soft, lint free

cloth or wiper dampened with water. For more aggressive contamination, a mild solvent

such as 70% isopropanol in water may be used. Use caution when cleaning and do not

oversaturate the wiper or otherwise flood the system with cleaning agents. Do not allow

liquid to enter the AC/DC power supply or the control electronics.

Instruments that have been used with biological samples or might otherwise contain

biohazards, toxic, carcinogenic, mutagenic, corrosive, or irritating chemicals must be

decontaminated prior to service work performed by the user or by Prosolia and its

affiliates. Please contact Prosolia to discuss decontamination of the system.

Always wear appropriate personal protective equipment (PPE), including gloves, lab

coats, and safety glasses when decontaminating and servicing the system. Adhere to your

institutions Chemical and Biological Safety Procedures when handling chemical and

biological hazards, decontamination solutions, and waste. Follow all local regulations for

the disposal of waste.

71

CAUTION Systems which are contaminated with biological, carcinogenic, mutagenic,


ATTENTION Systèmes qui sont contaminés par des dangers biologiques, cancérigènes,

mutagènes, corrosives, toxiques ou irritants doivent être décontaminés avant

l'entretien.

10.2 Preventative Maintenance

During the routine use of the DESI 2D system, the following items should be inspected on

a daily basis to ensure the system is operating properly.

Solvent supply tubing – Inspect tubing for damage, including kinks that may

affect performance of the system.

2D Stage – Home the stage and exercise it in both directions observing movement

for issues.

The following should be inspected on a weekly basis:

Electrical cables – Check cables for signs of wear or other damage. Replace as

necessary.

Exhaust tubing – Inspect the tubing for signs of deterioration from the spray

solvent. Replace tubing as necessary.

When the system is installed onto the mass spectrometer:

Gas Plug O‐Rings – Check o‐rings for wear. Replace as needed.

10.3 Spray Head Nozzle/Emitter Replacement

Please note that replacement of spray nozzle/emitter requires care and improper

installation can affect the quality of the experimental results. Nozzles and emitters are

delicate, handle with care.

1. Place the instrument in standby mode and ensure the high voltage is turned off

2. Turn off high pressure nitrogen gas to the system and allow time for the gas lines to

depressurize

3. Remove the spray head from the DESI 2D system

4. Remove the gas connection from the spray head and unscrew the nozzle adapter/nozzle

from the PEEK tee connector

72

a. If the spray head nozzle is being replaced, unscrew the nozzle using the 4 mm

wrench provided

b. Insert the new nozzle (with o‐ring) into the nozzle adapter and begin tightening

by hand. Use caution to prevent cross threading parts.

c. Tighten nozzle using the 4 mm wrench provided, until o‐ring is within the nozzle

adapter

d. Continue with emitter replacement/spray head reassembly

5. Remove and discard the old emitter from the PEEK sleeve

6. Inspect the o‐ring on the ferrule for signs of wear or cracking and replace if necessary

7. Using the 4 mm wrench provided, turn the spray nozzle clockwise until the rear of the

wrench flats is flush with the front surface of the nozzle adapter as shown in Figure 10.3.1

Figure 10.3.1 Position of the nozzle within the nozzle adapter for replacement of the emitter

8. Carefully insert the new emitter through the aperture in the tip of the nozzle until

approximately 3 mm is left protruding from the nozzle as shown in Figure 10.3.2

Figure 10.3.2 Emitter protrudes 3 mm from the nozzle

9. Carefully grip the emitter (avoid damaging the tip of the emitter) and the tip of the nozzle

between your thumb and forefinger to prevent the emitter from moving while inserting

the distal end of the emitter into the PEEK tubing sleeve

73

Figure 10.3.3 Insertion of the distal end of the emitter into the PEEK sleeve

10. Insert the sleeve and ferrule completely into the nozzle adapter and while holding the

ferrule in place, gently push the emitter further into the nozzle

11. The distal end of the emitter should be made flush with the end of the PEEK sleeve

protruding from the ferrule

Figure 10.3.4 Positioning of the emitter at the end of the sleeve and ferrule

12. Assemble the nozzle adapter with the new emitter onto the tee connector while keeping

the ferrule from moving

Figure 10.3.5 Assembly of the spray head

74

13. Upon tightening, the proximal end of the emitter will protrude from the tip of the nozzle

14. Using the 4 mm wrench, turn the nozzle counter clockwise until the emitter protrudes

approximately 1 mm beyond the tip of the nozzle.

Figure 10.3.6 Adjustment of the emitter protrusion using a 4 mm wrench

Figure 10.2.7 shows a disassembled spray head and its different components.

Figure 10.3.7 Disassembled view of the DESI 2D System spray head

Nozzle Adapter

Nozzle

Pt Electrode

Ferrule/Sleeve

PEEK TEE

75

10.4 Spray Head Cleaning

The spray head of the DESI 2D system can be cleaned if chemical contamination of the

spray head is suspected. Care must be used to avoid damaging the o‐ring seals of the

spray head. Simple cleaning of the outer surfaces of the spray head can be accomplished

by wiping the spray head using a wipe saturated in methanol. The spray head must not

be submerged in solvents.

A complete cleaning of the spray head requires disassembly and removal of the o‐ring

seals. All parts of the spray head can be cleaned by sonication in methanol except for the

electrode assembly. After removing the spray head from the system, follow the

procedure below to dissemble and clean the spray head.

1. Remove the platinum ‘liquid junction’ assembly from the tee by unscrewing the retaining

nut and set aside

2. Remove the nut and ferrule from the liquid entry side of the tee and set aside

3. Unscrew nozzle adapter from tee

4. Remove 3 x 1.25 mm o‐ring from the ferrule behind the nozzle adapter and discard

5. Unscrew nozzle from the nozzle adapter. Remove the 1 x 3 mm o‐ring from the nozzle

using pointed tweezers and discard

6. Remove spiral retaining ring from the nozzle adapter assembly by carefully prying end out

of groove with sharp tweezers of a small screwdriver and remove using tweezers or small

pliers. Use caution to avoid damaging PEEK parts

76

Figure 10.4.1 Removal of the spiral retaining ring (A and B) Carefully pry end of ring from groove at gap (circled) (C) End of ring pulled from groove (D) Using pliers to ‘unwind’ ring from groove (E) beginning to remove spiral ring (F) Ring removal near completion

7. Separate components of the nozzle adapter by twisting the outer ring while pulling pieces

apart. Remove and discard o‐rings from this assembly

8. Place the spray head components into a beaker containing methanol. Sonicate parts for

10 minutes.

IMPORTANT Do not sonicate the platinum ‘liquid junction’ assembly.

IMPORTANT The spray nozzle is delicate, handle with care. Do not drop or otherwise

bang the tip of nozzle against hard surfaces or damage may occur.

C

A B

F

ED

77

9. Remove components from methanol and allow to dry

10. Clean the platinum electrode by carefully rinsing only the tip of the electrode with

methanol using a spray bottle

When components of the spray head have been cleaned and allowed to completely dry,

the spray head can be reassembled. It is suggested that new o‐rings be used upon

reassembly.

1. Place new 7 x 1 mm o‐rings into the appropriate grooves on the nozzle adapter by gently

stretching the o‐ring over the part

Figure 10.4.2 Placing 7 x 1 mm o‐rings onto nozzle adapter

2. Place the slip ring component of the nozzle adapter over the barrel component with the

chamfer facing the o‐rings as in Figure 8.3.3

78

Figure 10.4.3 The slip ring should be placed over the barrel with the chamfered edge

facing the o‐ring to ease assembly

3. Push the slip ring component of the nozzle adapter over the barrel and o‐rings until it

seats against the shoulder of the barrel. A gentle twisting of the ring will help to slip the

ring over the o‐rings

4. Place a spiral retaining ring over the barrel of the nozzle adapter

5. The retaining ring can be started in the groove by gently pulling the end of the ring and

stretching it into the groove. The remainder of the ring can then be stretched into the

groove by gently pulling the ring into the groove working in a circle around the barrel

79

Figure 10.4.4 Replacement of the spiral retaining ring

6. Place a 3 x 1.25 mm onto the ferrule placed behind the gas adapter.

7. Continue assembly of the spray head as described in Section 10.3

80

10.5 Extended Transfer Capillary Cleaning

During normal use, the extended ion transfer capillary will become dirty and require

routine cleaning. While cleaning the capillary, it is important to handle the capillary with

care to avoid bending or otherwise damaging the tube. Damage to the tube can lead to

changes in the experiment geometry and can affect system sensitivity and performance.

Before removing the system, allow the interface of the mass spectrometer to cool.

1. Reduce the interface temperature of the mass spectrometer and place in Standby

mode.

2. Close the vacuum isolation valve on the mass spectrometer.

3. Using the 5 mm wrench provided, remove the extended ion transfer tube by rotating

the base upwards until free.

4. Carefully remove the o‐ring and ion sampling cone from the backside of the extended

ion transfer tube assembly.

CAUTION Systems which are contaminated with biological, carcinogenic, mutagenic,


ATTENTION Systèmes qui sont contaminés par des dangers biologiques, cancérigènes,

mutagènes, corrosives, toxiques ou irritants doivent être décontaminés avant

l'entretien.

When cleaning the ion transfer tube, use appropriate PPE and dispose of all waste

according to local regulations. For general cleaning, sonicate the capillary in an

appropriate HPLC grade solvent for the samples/analytes the capillary has been exposed

to. For more thorough cleaning, the following protocol can be used.

1. Sonicate in Alconox solution (prepared to the manufacturer’s directions) for 15

minutes with the tube pointed upwards in an appropriate container.

2. Rinse with clean tap water ensuring tube has been flushed.

3. (Optional) For parts with oxidation, sonicate in a Citranox solution (prepared to the

manufacturer’s directions) for 15 minutes with the tube pointed upwards in an

appropriate container.

4. Rinse with clean tap water ensuring tube has been flushed.

5. Sonicate in clean tap water for 10 minutes.

6. Rinse with HPLC grade water ensuring tube has been flushed.

7. Sonicate in HPLC grade water for 10 minutes.

8. Rinse with HPLC grade methanol.

9. Sonicate in HPLC grade methanol for 10 minutes.

81

10. Allow tubing to drain over the wash container. Dry accessible surfaces carefully with

lint free wipes.

11. Reinstall onto the mass spectrometer following the instructions of Section 6.3.

10.6 Fuse Replacement

Under normal operation, the fuses protecting the DESI 2D control electronics should not

need replacing. Should the fuses of the control electronics burn out, follow the procedure

below for proper fuse replacement.

1. Unplug the DESI 2D control electronics from the main power. Ensure there is no power

to the unit before continuing

2. Using the flat head screwdriver provided with the system, turn the grey fuse cover

counter clockwise until it springs out.

3. Pull the grey fuse cover out from the back of the control box. The fuse should come out

with the cover.

Figure 10.6.1 Removing fuse carrier from the fuse holder

4. Replace the blown fuse with the appropriate fuse for the voltage indicated on the

enclosure (Nominal voltage = 400V DC, Current = 1A, Fuse = 5 X 20 MM Type T or

equivalent (e.g. Littelfuse 0477001.MXP). See Section 11 for Prosolia part number.

5. Insert fuse and grey fuse cover back into the back of the control box. Using the

screwdriver, push the grey fuse cover into the control box while turning clockwise to

engage the cover back in place.

6. Reapply power to the control electronics.

If the fuses continue to burn out, this is an indication of a larger problem with the

electronics. Please contact Prosolia ([email protected]) for further assistance.

Insert screwdriver here

82

10.7 Decommissioning and Disposal

The DESI 2D system must be properly prepared prior to disposal/recycling of the

equipment. Spray solvents shall be emptied from the syringe and all tubing and disposed

of according to local regulations. Wearing the proper PPE, the instrument must be

decontaminated when exposed to chemical or biological hazards arising from samples or

the spray solvents used prior to disposal or recycling to minimize risk of exposure to

others. If the instrument cannot be properly and completely decontaminated, the

system must be disposed of as hazardous waste following all applicable laws and

regulations.

Please contact Prosolia for additional information or with concerns on the safe disposal

of the DESI 2D system.

83

11 Replacement Parts

The following spare parts that can be ordered from Prosolia for use with the DESI 2D

system. Please contact us for price and availability.

Spray Head

Item Part Number Description

1 P100‐00031‐01 Oring, 3X1.25mm, NB70

2 P100‐00087‐01 Retaining Ring, 3/8” Ext. Spiral

3 P100‐00088‐01 Oring, 3X1mm, NB70

4 P100‐00089‐01 Oring, 7X1mm, NB70

5 P200‐00035‐03 Nozzle, Spray Head, SS, Rev C

6 P200‐00036‐02 Spray Head Gas Adapter Rev B

7 P200‐00101‐01 Slip Ring, Nozzle Gas Inlet

8 P300‐00001‐01 Tee, Microtight, PEEK

9 P300‐00002‐01 Nut, Microtight, 5/16‐24, PEEK

10 P300‐00003‐01 Ferrule, Microtight, 0.025”, PEEK

11 P300‐00004‐10 Sleeve, Microtight, 0.180mm, PEEK, 10 Pcs

12 P300‐00005‐01 Nut, Short Vacutight, 10‐32 UNF, PEEK

13 P300‐00006‐01 Ferrule, Vacutight, 1/16”, PEEK

14 P800‐00057‐01 Assembly, Banana Plug

15 P900‐00051‐05 Emitter, 0.05 X 0.15 X 40, Fused Silica, 5 ea

84

Capillary Extension

Item Part Number Description

1 N/A Waters Ion Sampling Cone (Contact Waters for replacement)

2 P100‐00139‐01 Oring, 10X1.5, Viton

3 P800‐10000‐28 Assembly, Bazooka Inlet ‐ Waters Xevo 2D

Tools

Part Number Description

P900‐00101‐02 Wrench, 4mm X 5mm, Open

P900‐00102‐01 Wrench, Hex Head, Metric, Set

P900‐00112‐01 Insulated Hex Driver, 2.5 mm

P900‐00113‐01 Insulated Hex Driver, 5/64 in

P900‐00114‐01 Cutter, Fused Silica

Omni Slide Sample Surfaces


P900‐00015‐05 Slide, Omni Slide MT‐96, 5 pk.

P900‐00016‐05 Slide, Omni Slide MT‐IMG, 5 pk.

P900‐00018‐00 Slide, Omni Slide Micro‐24, 100 pk.

P900‐00020‐00 Slide, Omni Slide Micro‐IMG, 100 pk.

Chemical Standards


P900‐00307‐01 Bradykinin Standard, 1 Milligram, Dry

P900‐00302‐01 Sharpie, Fine Point, Red

85

Plumbing/Fluidics


P300‐00200‐01 Syringe, 250 uL, Removable Needle, 22/2”/3

P300‐00030‐01 Union, Microtight Adapter,1/16”X1/32”, Red PEEK

P300‐00015‐01 Union, 1/16”X0.007”, SS

P300‐00004‐10 Sleeve, Microtight, 0.180mm, PEEK, 10 Pcs

P300‐00018‐10 Sleeve, 1/16”X0.007”, FEP, 10 Pcs

P300‐00013‐01 Tubing, 1/16” X0.030”X40mm, FEP

P300‐00011‐99 Tubing, 0.05X0.150X1000 MM, FS

P300‐00047‐01 Tubing, 1/32”X0.010”X1.5 M, PEEK

P300‐00010‐03 Tubing, 1/16X0.30”X1.5 M, ETFE

P300‐00016‐01 Nut, Fingertight, 1/16”, Delrin

P300‐00017‐02 Ferrule, Fingertight, 1/16”, Tefzel

P300‐00029‐01 Ferrule, Fingertight, 1/32”, PEEK

P800‐00271‐01 Plug, Gas Port, Xevo

P100‐00086‐05 Oring, 2.5X1mm, NB70, 5Pcs (small o‐ring for gas plug)

P100‐00088‐05 Oring, 3X1mm, NB70, 5 Pcs (large o‐ring for gas plug)

Electrical


P400‐00349‐01 Fuse, 5 X 20 mm, 400VDC/1A, Type T

P400‐00037‐01 Cord, USA/Japan, 10A/125V, 2 M

P400‐00038‐01 Cord, UK, 10A/250V, 2.5M

P400‐00039‐01 Cord, Europe, 10A/250V, 2.5M

P400‐00040‐01 Cord, Australia, 10A/250V, 2.5M

P400‐00302‐01 Cord, Swiss, 10A/250V, 2.5M

P400‐00311‐01 Cord, China, 10A/250V, 2.5M

P400‐00075‐01 USB Cable, 10 ft

P400‐00042‐01 Cable, CCTV, BNC‐BNC, 10 ft

P400‐00094‐01 2‐D, 25 Pin D‐Sub Connector Cable

P800‐00013‐03 Assembly, Camera, WAT‐704R w/Mirror

P800‐00131‐02 Assembly, LED Lamp, 2D Waters

P400‐00045‐02 Video Capture Card w/Ferrites

P800‐00066‐02 Waters High Voltage Cable

P400‐00074‐06 Assembly, I/O Cable, 2‐D Xevo

86

12 Troubleshooting

This section details information on correcting issues or malfunctions with the DESI 2D

system. If you experience a malfunction, this information will assist in identifying and

correcting the problem. If you experience a problem not discussed in this section, please

contact Prosolia’s technical support for more information.

Hardware Errors

The 2D sample stage has been designed for years of trouble free use. Common issues

seen with the stage include binding due to damage, inability to home the stage in one or

both axes, and erratic movements when the motor control cables in not completely

plugged into the system. In all cases, these malfunctions do not present a hazard to the

user and will not likely lead to permanent damage to the system.

If you experience a malfunction, note the conditions leading to the malfunction including

which axis is affected, if the error occurs during a particular function (such as homing),

and any other relevant details before contacting Prosolia’s technical support for

assistance.

Common issues and suggested solutions are located below.

No power

Power status lamp not lit

Check power cable is properly inserted into the control electronic Ensure power cable is plugged into live power outlet Check that power switch is in the ON position Check fuses

Stage motion stalls Check for obstructionEnsure motor control cable is secured at both ends

Stage motion is erratic Ensure motor control cable is secured at both ends

Stage does not stop trying to

move when the home position

is reached

Home limit needs adjustment. Contact Prosolia for details.

87

Communications Errors

The DESI 2D system must be in constant communication with the control software for

operation. The connection and communication requirements are discussed in Section 7

of this manual and in the Omni Spray Ion Source 2‐D Automation Software Installation

and Reference Manual.

Communications Error Check that power switch is in the ON position Check that USB is properly inserted into both the control electronics and the computer Ensure the control electronics are plugged into a root USB port, not through a USB hub

Experimental Errors

Some common experimental issues are noted below. For more detailed troubleshooting

information on system performance as well as application dependant information,

application notes, and other reference information, please visit Prosolia’s website at

www.prosolia.com.

Synchronization error – sample

moves to analysis position

before data acquisition begins

Adjust pre‐acquisition delay parameter (Pre_Acq) in the motion parameters window to a shorter time period

Synchronization error – sample

moves to analysis position after

data acquisition begins

Adjust pre‐acquisition delay parameter (Pre_Acq) in the motion parameters window to a longer time period

Synchronization error –

Instrument not ready when

trigger is sent

Adjust post‐acquisition delay parameter (Post_Acq) in the motion parameters window to a longer time period

Weak/no signal for

recommended standards

Ensure the syringe pump is ‘ON’ and the spray solvent is flowing Ensure nitrogen gas pressure is sufficient and gas is flowing Check that the capillary emitter is protruding out of the nozzle according to the recommendations in Section 5.3. Ensure all operating parameters are similar to the recommendations in Section 9.1 Ensure instrument is functioning properly (tuned and calibrated

If the above suggestions do not solve issues related to the DESI 2D System or control

software, please contact Prosolia ([email protected]) for further assistance.

waters desi system operator's guide

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