Volume 2, No. 4 OCTOBER - DECEMBER 1997

CENTRAL INSTITUTE OF BRACKISHWATER AQUACULTURE, CHENNAI I FREEZE-DRIED MlCRCbPAKl'ICULATE DlET FOR

FARLY AND POST-LARVAL STAGES OF TIGER SHRIMP PI..NAk'II.S M0NOiK)N

for early a d p tges of tiger shrhnp P don J

R-h Higt

Susupribit~ryno- of dilkmnl aquatic cmhxarn to whitc spd discsrc virus

Nurrcry and p w a n t cultnac of scabass in farmcrs' ponds

\hsitors - Mcet iw Human Raw lrnl

Training ARsiNErm- SMNerws

A p p a i m

Pnnnotion Woparflcubte keds (or poslbwe of Penseus monodon

Artificial diet playa an important mle m the production of penmeid shrimp r&d m the hatchcnep. M i l i ~ i a l d i e are being increasingly used to supplcmcnt the live feeds lor feeding early and post-larval stages of shrimp.*Thc digestive syrtan of a r t y and post-l~rvnl

1 rtagofshrimpnxpimladaudfrah diet f a easier d i g d i m and a.&mibtkm For this rcsson. IIVC food orgsni.am nrch as phytoplankton and zooplankton arc successfully used for rearing shrimp I-. Thc -sing of live fc4.r. especially that of the brine shrimp ( A m i a ) and che d n t y laocistcd with the mass production of plankton cultures. have led to rupplcmcntation with particulate diets. Freeze-dry~ng technique has helped in preserving the freshness and preventing the

d m a ~ t i o n o f p c p a r e d d i i b a I m p extent. Fromadried dicta an . lhercforc. more successful in rearing larvae than (hacdiccspmwmd~lhmvirc.

Following the s u c c e s ~ in the preparation and testing of a micrcyarticulate foal f a posllrvm of white shrimp. I'emur id io t s , by the Institute, another micro-particulate d id was developed using freeze-drying method and tested on early and post-larval stages of tiger shrimp. P m a d m . Thc diet conaisted of indigenour raw materials. namely. Aah (anchovy). molluscs (squid and clam). soy flour, wheat flour. egg (duck) and mddttives such as vitarn~ns. minerals. lecithin, cholesterol, aminoacids. m I i m and yeast. The proximate composition of feed c o n s i d of crude

Advanced postlarvae of tiger ahrlmp P rnonodon The fol lowing vis i ted the

naur ratc micr it uvt ---s

size of 30 nun in 35 days with a survival rate of 82%. Dunng thc f M 10 days of rearing. Arfmia nauplii and minced shnmfllsh meat werc offend ad l ibit~m and during 11-3 5th day, the dally - ra t~on of minced rhrimplfish meat was given @ 2 W of stocked h ~ o m s s

- The n m c q - n a d fry of 30 mm 1 1.25 g, stocked in a 0.4 ha pond, nached a size of 200 g in 100 days. The stocking rate was 5 0 0 0 h and trash fish was offered as feed (ii! 10?6 of stocked h~omass. - In another trial. in a 0. I ha pond, with s e m stocking density and feeding. fry with an initial size of 10 mm have grown to 85 g in 58 days. '

VISITORS

nlo18 con' cull cnsc larv

te in (46.13%). l ipid (11.7%). cnrhohydrate (14.4?/0). crude f ibre (0.42%) and ash (10.9%0/a). Fresh meat of anchovy, squid, clam and duck e a w m i n d ~ v i d u a l l y f reeze-dr icd a t -43%.

vdered separately in a cyclotech mill sicvcd throuph a 45 micrnn sieve, All e r i n p r e d ~ e n t n werc similarly ninad rind R C I V ~ .

The diet was tested either alone or i n a o o m h ~ n a t i o n o f I : I ra t io with laboratory cultured unicellular diatoms (('lmrronrnr sp . and Skrlrtonrn~n np.) uslnp 25.000 lint proto7ocnl strip of P

rcdon ntookbd in a 500 Illre tank. Ihe Irol larvae wcrc red only wlth diatom 'ure @iJ 40,000 cells per ml. In the : of experimental pr t~cula te dict,the

.... whi 0 04 2 0 s f c d

ne were fed (& of 0.08 nip per Iowa, Ie the comhinl~tion dict was given (a 1 mg per Inntn plus a concentration of 100 cells ofdiatoms/ml. The larvae with m i i e d die t a t ta ined the

t-larva first stagc (PI,-I) i n 8 days I RS% uurvival compared to 62.5% ivnl in the oarr ofoontml diet. Those wi th micro-die t took 9 davs to )me PL-I with a &vnl of 40?/0.

Tho same diet with a particulate size of 200 micmns was tested for rearing first postlarva (I'L-I) to PI.-20 stagc. 1:reshly hatched hrine shrimp (.4rfrn11d

~ l i i were used as the contml diet. The of survivnl for post-larvae fed w ~ t h ndie t was S7"& at ill-20 SIO~C. while IS 41.% in the oam of h m Tbd \v~rh

C l l l l l

I'l,-i and post

were healthy and withstood 150 ppm formalin tent without any morta l~ty . Thus. the freeze-dried m~cro-particulate diet developed indigenously could he nuccersfully uscd for rearing early and pst-larval stages oftiger shrimp. It is hoped that using micro-part~culatc diets in commercial shrimp hatcheries would work out to be less expemive and greatly reduce the dcpendcnce on costly live-feeds.

This article was prepared hv Dr.Syed Ahamad Ali. Senior Scientist

RESEARCH HIGHLIGHTS

Susceptibilitynolerance potential of different aquatic crustaceans to white spot disease virus

I . t ~ h o r n t ~ r y cvperiments were conducted on lnductlon of white spot disease in different species of aquatlc decapod crustaceans to study their susceptihil~'\. and tolcrancc potential for this \r~rus ~ntcctlon. Results indicated that the mud crahs S q l l o serrata and S. hn lp rha r i ca and spiny lohsters Panu/im.r ornahr~. P. p ) / v p l ~ a p s and l? how~arus were tolerant to the white s p t d ~ m m virus.

Nursery and growout culture of seabass in farmers' ponds

The following feed back infonat iob c o l l e c t l tiom prlvate fish farmers on nurser?. and gmw-nut rearing of hatchery-raised f n of seahass (Lotes

Muttukndu ExperimenGl Station: - Dr. A. Puma Chandra Rao, Msnager (Rural Department). Stnte Bank of Inma, V~javawada, 9 December 1997.

- Dr. Horny Emara. Director, Dr. Fatima Aly AMel k e k and Dr. Salirnan llamed, Scientists, National Institute of Oceanography and Fishenes. Alexandria. Egypt, 15 December 1997.

ENGAGEMENTS

Dr. G . R. M . Rao, Director, attended the following Meetings / Work s h o p 1 Seminars:

- Colloquium on 'Key issues relating to thc fisheries sector' at h4I'EI>A, Kochi. 14-15 Octoher 1997.

- Wnrkshop on Environmental Impnct Assessment of Aquaculture Enter- prises. orpnised hy Raj~v Gandhi Centre for Aquaculture. at Chemi . 10 December 1997 and presented a pper.

- The National Agricultural Techno- logy Rqlect (NATIJI meeting at ICAU, New Dclhi. 22 December 1997

S h r ~ S. S r i n ~ v a s a g a m , Senior Scientist, visited Pun Research Centre of CIDA. Piki and had discussions with the Secretary. Dept. of Fishcries and Anirn@ Flushandy and the Director. Dept. of Fishcr les , Govt . o f Orissa , on collaborative research work in shrimp. seahass and mud crab, 26 August to I September 1997.

lrol d ie t . The average weight o f cdcm!/'.') is @\'enhlow: Dr. Ma thew Abraham, Senior !O was 14.4 mg in case of mlrodiet - At a stocking density of 1000 Scient is t , a t tended the Orienta t ion 16.6 m g with control d ie t . The nca.lcuhic metre in cement tanks, fry Workshop o f NATP a t NAARM. ,

-larvae in PI,-20 stage in hlth oases uith an initial site of 9 mm at- a Hyderabad. 25-26 w b e r 1997

Dr. A. R. Thirunavukkarasu. Senior Scientist, delivered lecturrs on 'Brackishwater fish farming and fish breeding. at CAS In Marine B~ology. Parangipettai. Annamalat University. 17 Octoher 1997. He a lso attended the NATI' Workshop at CMRI, Jodhpur, 24-25 October 1997.

Dr. S . S . Mishra and S h r ~ M. Shashi Shekhar, Scientists, presented ppn in the National Workshop on Fish and Shellfish Ilealth Management. at CIFA, Rhuhaneswar. 18-19 Novemher 1997.

1%. K. 0. Joscph, Senior Scientist. presented a papcr In the Workshop on Environmental Impact Assessment of Aquaculture Enterprlscs at Chennni, 10 kcemher 1997.

Dr. M. Muralidhar, Scientist . attended the Workshop on Identification and Assessment of Acid Sulphatc Soils and their Impacts on Shrimp Roductlon in I n d ~ a . at Col lcge of' Ftshertes, Mangalore. 10-12 1)ocemhcr 1907.

MEETINGS

The meet ing on the Institute's Consultancy I'roposal I'or transfer of technology in culture of mud crah and scahass t o Rajiv Gandhi Centre for Aquaculture (KGCA), Mayiladuthurai. Tamil Nadu, was held at CII3A. Chcnnai. 24 October 1997. S h r ~ K. 13 1'1lla1, Chairman. Shri V Venkatesan. I>irector. Dr Santhanakrishnan. Jolnt I)lrcctor, MPEDA and Shri R. Ganapathy. Ifqect Director. HGCA. attended thc mect~ng

Dr. G . K. M Roo. Director. Dr. Mathe\\* Ahraham. SlShri M. Kathirvel. S. Srlnlvasagam and Dr. S . Kulaackarapandian. Senior Scientists. pntc~patcd In thc dlscusstons

In collohoration with C lnA, the Nattonal Kcsearch Ccntre on O~lpulm. Kolleru. Andhrs I'radcxh, orpanlsed a Workshop for I'lanning ond I'rcparutlon of I'roject Proposals under NA'l'I', at TANIIVAS. Chennai, on 27 Octoher 1997. TX ( i . K M Kao, Dlrcctor. CII3A. Ilr . P R c t h ~ n a m . Illrcctor. NRC on Ollpalm. I3r K . I . . Kaul. O f f ~ c e r on Special l)utv. NAI'P. ICAR. I'rincipal Invcstlgatorr and Co- principal Invmt~gutors of dtflicrent ICAK Inst~tutcs

and Stale Agricultural IJniversities pflictptcd

A meeting of Pr ino~pa l Invest igators snd Co-principal lnvcstigotors of ICAR Institutes and Stilte Agricultural I J n ~ v e r s i t ~ e a for the preporution of project proposals undcr NA'I'I' I ' rogramn~c, on I '~sh/shrltnp hroodstock dcvclopmcnt and hrcedlng tlnnder captive conditions and shrimplfisl~ health management, was held at CIl3A, Chcnnt~i. 15 Nov~mlxr 1997.

Ilr . G. R . M. Rao, Director. Dr. Mathcw Abraham. Dr. Sycd Ahnmad Ali and 1%. K. (ioptmthan. Scnior Scicntirls, pctrt~cipated In the dincussions on the Institute's research programmes and octtvttlcn wtth Mr lml1a7 Ahamcd. Senlor I'roject Offtccr and Kes~dent I<eprcucniatlve from Ilhaka. 13angladesh

NATP YIbhshop Len to ngM Dr K L Kaul. Oficer on Speual duty NATP. Dr G R M Rao Dmdor. ClBA and Dr P Rethmam Dmctor. NRC on 011 Palm

and Mr Acnwn Ateng. I~conom~at. South Enst Rcgton of Ray o f I3engnl I'rogrmmc. Chennai, 7 Novemher 1907

HUMAN RESOURCE DEVELOP- MENT

I>. P. Kishore Chandra. Scientist. a t tended a t r a ~ n i n g programme on Computerined Project Management. organised by NATI' Monitoring Unit at IASRI. New Dclhi. 20-25 Octohur 1997.

Dr. K. K. Vijayan, Scientist (S.S). completed a one year advanced training programme, sponsored hy Dep t . of Hiotechnology, Govt. of India, at Gulf Coast Research Laboratory. USA and joined the Institute on 29 Decembcr 1997.

11 Tminan mining 1- :uicnn. of Tam

>fneahaa. It 1 M.Q. (Aq :ma hrc.1 3mmi. <

md dcmonmt~ the follow

_-:---.-I C..-.

rations wm ring at the 8.-.

fmm Firl iMe. I*. I

~i l Ndu. im I

TRAINING I.ectuns a

arranged for *'-!Iblkd~ /<X~III.EIMI .IULI.III.

laia .surf ~f Fihrim. Ihc b d i n g

Iseptnnha 1997. usculture) mdanlr fmn

Collelqa. Nsnd.mm,

m xhrimp hillchuy

cklnhcr 1997. Tmn chs Fihsrier Star

itulc. I*. of Fisheries. .mn. 01 Tmnil Ndu. on duimp4'iuNmh hrobding, mtiftial bat1 pqnml"R mvimmncnlsl nonibring and skl lf iM1nfi l k l t h ~ q e m ~ n t . I5 IZonnha 1997. STAFF NEWS - Shri N. Ramesh, T-1 as T-2 from 6 - 6 dudcnb finm I k p . of Applied Appoinbnent August 1995.

Aquscultura, l b b t u l a h llnivmity. , M~~ p. ~ i l * ~ ~ k h , , , as ~ h t i d ( s I 1 - Shri S. Ssminsthan. T-l as T-2 from

Rhopal. m Inst~tute's resarch d %ter Cm-tion lhginoming), 1XJJJnU'JV 1%.

ttintias, 22 lhmnhcr 1997. at Chcrmai. 3 1)cccmhcr 1997. Transfer 17 d- fmm ~~~k~~ ~rfshi - Shri K. Amhnsankar, as Scicntid Shri P. M. AWul Kadir. T-5, from

ir+palh allcRe. ( h i m 1 Nucribon). at C h i . 4 Headquarters. Chcnnai to Narakkal l becmher 1997. Research Centre. relieved on 14 August

.I.bup,iri. oa dullfiMmfish hiltchy. 1097. fmd prapntlon and hcalth Promotion -1.26 I)amnher 1997. - Shri K. I~knpvan. T-4 as T-5 from I3 Retirement

Ikccmhcr 1995. Shri K. Rajasekaran Nair. Senior ARSNETBRF EXAMINATION . _ Shri S, SiwpMm. T-Il-3 as T-4 Clerk. rctired on supcmnnuation. on 3 1

The Institute conducted the from 14 F c h q 1995. Oclobcr 1997.

ARSiNKTISHF l.:xamination. 1997. at - S~ D. RajaMq T-11-3 as T-4 from Participation i n SpOftS - E A R Inter-Institutional Zone Ill Tournament 1997

3 s c i e n t ~ f i c (SIShri D. Namyanaswamy, V. Chellapandian and K. Ponnusamy). 10 technical (SIShri R. Elangovan. A. Vasanthakumar Charles, S. Rajkumar. Joseph Sahayarajan, Marella R a v ~ . A. Nagavel, Ashok Kumar, K. I'aranthaman, N. Ramesh and N. Jagan Mohan Raj), 5 administratin: (SIShri P. K. Msnimandiram, K. Kandamani. S. Pari. A. Manoham and P S r i h t h ) md 12 supporting (SIShri T. V. Shaji. M. D. Surcsh, V. Kumar. E. Manoharan. C. S m a ~ n m , R. KI-1. S. Sclvabshq D. Scnthil Kumar. C. Reghu, A. Paul Peter. K. lndirakumar and Kanaka h s a d ) , personnel took part in ICAR In*-lnditutional S p a i ~ MCCI (m I-). at Coimhatore, durine 4- 10 Novemher 1997. Shri R. Kumnresan reccivcd two

Shn R Kurnansan. S u w Stall - SS GI I. rsaewng the oefhhg.le Irom prizes from Shri B. K. Chauhan. Shi B K C h u h n . Ssosbry. ICAR. & hkhmg m lhnl poshan m

1~mhsraa .ndkmgjunpevern Secretary. ICAR. for f i ishing third in ~ ~ m b c ~ c c a d l o g j w q e v c n ~ s .