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Travis Cranmer
Effect of grafting on the efficacy of bio-pesticides
against root diseases in hydroponic tomato production
1) Compare bio-inoculants for suppression of root-borne disease
2) Evaluate bio-inoculant impact on whitefly and spider mites
3) Evaluate the effect of grafting and application timing on disease suppression efficacy
Treatments• Bio-inoculants• Fungicide control• Infested control• Non-infested control
Evaluating product efficacy
Greenhouse experiments
Design• Randomized block• 4 replicates – 3 plants
Evaluating product efficacy
Greenhouse experiments
Bio-assay timeline
Propagation
Grow blocks
Grafted production on slabs
Week 1
Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
Bio-assay timeline
Propagation
Grow blocks
Non-grafted production on slabs
Week 1
Week 2
Week 3
Week 4
Week 5
Week 6
Week 7
• Slabs roots were rated on a 1 – 5 scale where:
Evaluating product efficacy
0 = no rot
1 = <20% root rot with good root development
2 = 20-40% root rot
3 = 40-60% with poor root development
4 = 60-80% root rot with poor root development
5 = >80% root rot and very poor root development
=Evaluating product efficacy
• Disease Severity Index differences between products
0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
Roo
t Rot
Dis
ease
Sev
erity
Ind
ex (
1-5)
Komeett
Komeett/Maxifort
=Evaluating product efficacy
• Disease Severity Index differences between products
0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
Roo
t Rot
Dis
ease
Sev
erity
Ind
ex (
1-5)
Komeett
Komeett/Maxifort
=Evaluating product efficacy
• Disease Severity Index differences between products
0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
Roo
t Rot
Dis
ease
Sev
erity
Ind
ex (
1-5)
Komeett
Komeett/Maxifort
0
10
20
30
40
50
60
70
80
90
100
Roo
t D
evel
opm
ent
(%)
Komeett
Komeett/Maxifort
Evaluating product efficacy
• Root development differences between products
0
10
20
30
40
50
60
70
80
90
100
Roo
t D
evel
opm
ent
(%)
Komeett
Komeett/Maxifort
Evaluating product efficacy
• Root development differences between products
0
10
20
30
40
50
60
70
80
90
100
Roo
t D
evel
opm
ent
(%)
Komeett
Komeett/Maxifort
Evaluating product efficacy
• Root development differences between products
0
10
20
30
40
50
60
70
80
90
100
Roo
t D
evel
opm
ent
(%)
Komeett
Komeett/Maxifort
Evaluating product efficacy
• Root development differences between products
• Currently conducting trials on different slab substrates
• Evaluate all products on cucumber
• Long-term trials – 5th cluster and harvest data
• Explore other application methods such as slab or block saturation
Future work
Substrates, cultivars and application methods
This project was funded in part through Growing Forward 2 (GF2), a federal-provincial-territorial initiative. The Agricultural Adaptation Council assists in the delivery of GF2 in Ontario.
Anissa PoleatewichRose BuitenhuisMichael BrownbridgeTaro SaitoGillian Ferguson (OMAFRA)Talia PlaskettJessica PinderJames Tiovonen
Colla b ora tors a nd re se a rche rs
Acknowledgements
Ashley SummerfieldMatthew CzerwinskiLitza CoelloAngela BrommitUpeksha NanayakkaraProplant Propagation
