the purest probiotic on earth tm -...

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THE PUREST PROBIOTIC ON EARTH

WITH INNOVATIVE APPLICATIONS AS:

HEALTH AND NUTRITION

FOOD SPECIALTY

TM

THE PUREST PROBIOTIC ON EARTHTM

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WHAT IS PROVIOTIC?

Plant based good bacteria (L. bulgaricus GLB44) Extracted from the Snowdrop flower in the mountains of Southeast Europe Grown in certified organic vegetable juice. Contains only L. bulgaricus GLB44 and traces

of organic vegetables. There are no carriers, no fillers, no preservatives, no artificial ingredients.

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1. HUMAN HEALTH AND NUTRITION PROVIOTIC AS A DIEATARY SUPPLEMENT

THE ONLY PROBIOTIC THAT IS DISCOVERED ON A FLOWER AND GROWN IN JUICE Certified organic 100% vegan No major allergens (lactose free, gluten free, soy free, nuts free) No artificial ingredients No GMO No bloating (homofermentative) Researched by Harvard Medical School scientists

COMPARISON WITH KEY COMPETITORS

BIOGAIA CULTURELLE

BIO -K PLUS (capsules)

GARDEN OF LIFE

ULTIMATE FLORA

DR. OHHIRA’s

Major Allergens

NO MAJOR

ALLERGENS SOY

GOODBELLY FLORASTOR ALIGN

LACTOSE LACTOSE LACTOSE SOY

LACTOSE AND SOY

LACTOSE AND SOY

LACTOSE

Animal Products

ANIMAL PRODUCTS

ANIMAL PRODUCTS

ANIMAL PRODUCTS

ANIMAL PRODUCTS

ANIMAL PRODUCTS

BEE PRODUCTS

ANIMAL PRODUCTS

Artificial Ingredients

NO ARTIFICIAL

INGREDIENTS

Triglyceride Magnesium

Stearate Titanium Dioxide

Magnesium Stearate Titanium Dioxide

Sodium Ascorbate

Silicon Dioxide

Magnesium Stearate

Trisodium Citrate

Magnesium Stearate Titanium Dioxide

Undefined Glycerin

Carrageenan

Magnesium Stearate

Maltodextrin

Triglyceride Sodium Dioxide

NOT ORGANIC

Certified Organic

NOT ORGANIC

NOT ORGANIC

NOT ORGANIC

NOT ORGANIC

NOT ORGANIC

NOT ORGANIC

NOT ORGANIC

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HARVARD MEDICAL SCHOOL AND BRIGHAM AND WOMEN’S HOSPITAL Researchers: Dr. Onderdonk, Dr. Fichorova Inhibition of E. coli, Listeria, Salmonella in raw foods with ProViotic Eradication of H. pylori in infected patients with ProViotic Control of vaginal infections with ProViotic

SCIENCE AS A CORE COMPETITIVE ADVANTAGE

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Harvard Medical School Inhibition Test: Inhibition of E. coli 0157:H7

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GROUP A:

Alcohol Free Beverages

Harvard Medical School Inhibition Test: Inhibition of Salmonella typhimurium

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Confocal Microscope Images of How ProViotic

Destroys E-coli Biofilm

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Confocal Microscope Images of How ProViotic

Destroys E-coli Biofilm

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Apple Cider Organic Alcohol Free

% Inhibition using G-LB-44 @ 1X

Inhibition Rate

Organism Strain

Acinetobacter baumanii ATCC 19606 >99

Enterococcus faecalis ATCC 29200 >99

Enterococcus faecalis ATCC 29212 >99

Enterococcus faecium ATCC 51559 >95

Esherichia coli ATCC 25922 100

Esherichia coli ATCC 35150 >99

Listeria monocytogenes ATCC BAA-751 100

Listeria monocytogenes Clinical Isolate 100

Psuedomonas aeruginosa ATCC 27853 100

Salmonella typhimurium Clinical Isolate

Shigella sonnei ATCC 325931 100

Staphylococcus aureus ATCC 12600 >99



SUMMARY OF PATHOGEN INHIBITION TESTS

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More products in 0.0% CONTINUOUS RESEARCH ON GUT PATHOGENS WITH NOBEL PRIZE WINNER FOR MEDICINE DR. MARSHALL

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GROUP B:

Ultra-Low-Alcohol Beverages 0.45%vol.

2015: First Human Trial – 22 patients Eradicating of H. pylori with 92% Success Rate

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Apple Cider Ultra-Low-Alcohol 2015: Second Human Trial – 52 patients

Eradicating of H. pylori with 92% Success Rate

To evaluate the effect of Lactobacillus

delbrueckii subsp. bulgaricus GLB44 in

commercially available form on Helicobacter

pylori infection in combination with a proton-

pump inhibitor.

Methods

Conclusions

Lactobacillus bulgaricus GLB44 - promising results in

Helicobacter pylori infection

Bibliography

Fifty two patients (57.7% female) at mean age

43.52±13.4y were enrolled in our study after

esophagogastroduodenoscopy. All patients

were Helicobacter pylori (HP) positive

diagnosed either by rapid urease test, stool

antigen test and histology examination, or by a

combination of these methods. Ten (19.2%) of

the patients had HP eradication at some point

in the past. Every patient was given

Lactobacillus bulgaricus GLB44 (capsules and

tablets) in daily doses of 15x109 living cells

together with a proton pump inhibitor

(rabeprazol, pantoprazol, esomeprazol or

omeprazol) 20 mg bid for 7 days, followed by

GLB44 alone for 3 days at the same dose. All

patients were retested for HP after at least 43

days.

1. Boyanova, L.; Stephanova-Kondratenko, M.; Mitov, I. (May 2009). "Anti-

Helicobacter pylori activity of Lactobacillus delbrueckii subsp. bulgaricus

strains: preliminary report". Letters in Applied Microbiology 48 (5): 579–84.

After the administration of Lactobacillus

bulgaricus GLB44 92.3% of all HP positive

patients had negative HP control tests

(p<0.001). Four patients were still HP positive

and one of them had previous unsuccessful

antimicrobial eradication course.

Lactobacillus delbrueckii subsp. bulgaricus

has in vitro inhibitory properties against H.

pylori.1 Per oral route of administration of

adequate probiotic dose in combination with

PPI is a promising treatment modality in HP

positive patients.

Objective Results

E-mail: [email protected]: None

HP positive beforeGLB44 HP positive after

GLB44

100%

7,7%

HP/+/pos. patients before and after Lactobacillus bulgaricus GLB44 + PPI course

Heartburn Epigastricpain

Dyspepsia Bleeding Other

32.70%

11.50%

48.10%

3.80% 3.80%

Clinical Presentation

Yana Valerieva1, Borislav Vladimirov1, Ivan Terziev2, Lyudmila Boyanova3

1 Clinical Centre of Gastroenterology, 2Department of Pathology, University Hospital “Queen Joanna – ISUL”, Sofia, Bulgaria3 Department of Microbiology, Sofia, Bulgaria

Medical University of Sofia

0%10%20%30%40%50%60%70%80%90%

HP Tests Used

Diagnostic test

Control test

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Grape Cider Ultra-Low-Alcohol PROVIOTIC HAS THE STRONGEST PATHOGEN

INHIBITION PROPERTIES

55.9%

22.5%

15.0%

100.00%

100.00%

100.00%

100.00%

78.4%

0.00%

0.00% Inhibits

(no rates found)

No information found








BIOGAIA CULTURELLE

BIO -K PLUS (capsules)

GARDEN OF LIFE

ULTIMATE FLORA

DR. OHHIRA’s GOODBELLY FLORASTOR ALIGN

E. coli

Salmonella t.

Listeria m.

S. aureus

A. baumanii

E. faecalis

E. faecium


Inhibits (no rates found)



PATHOGEN INHIBITION RATE IN VIVO

Dr. Onderdonk Harvard

Medical School Professor

PROBIOTIC STRAIN RESEARCH

Dr. Casas University

Laboratory at Lima, Peru

Dr. Gorbach, Dr. Goldin

Tuft University

Dr. Millette Université du

Québec, Canada

Dr. Mulin Lund

University Sweden

Dr. Delmasso University of

Nice France

Dr. Rao University of

Toronto Canada

Dr. Brenner University Feinberg School of

Medicine, USA

Dr. Smith Renew Life

Florida, USA

Dr. Ohhira Okayama

University, Japan

90.0%

23.5%

0.00% 0.00%

100.0%

84.7%

75.3%

73.5%


































Inhibits (no rates found) 100.00%

100.00%

99.86%

99.68%

77.2%

no information found

98%





P. aeruginosa

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Pear Cider Ultra-Low-Alcohol PROVIOTIC 35 CAPSULES

Flavor free 100% Vegan (plant cellulose capsule) Certified organic No major allergens (lactose free, gluten

free, soy free, nuts free) No GMO No bloating (homofermentative)

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GROUP C:

Low-Alcohol Beverages 4.5% vol.

PROVIOTIC 30 TABLETS

No other ingredients (100% Pure ProViotic Tablet)

Blendable tablet (perfect for smoothies) 100% Vegan Certified organic No major allergens (lactose free, gluten


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Apple Cider Low-Alcohol 4.5% vol. PROVIOTIC TRAVEL PACK – 10 CAPSULES

Flavor free 100% Vegan (plant cellulose capsule) Certified organic No major allergens (lactose free, gluten


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PROVIOTIC FEATURED IN:

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Pear Cider Ultra-Low-Alcohol OPRAH’S CHOICE

FEATURED IN O-MAGAZINE

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CORE TARGET MARKET

ProViotic Product Core Target: 1) Women 20 to 65 2) Health conscious 3) Nutritious conscious – focused on organic food 4) Weight conscious 5) Focused on clean eating lifestyle 5) 6) Would not appreciate allergens in the food

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Пробиотик

Canada: Ecotrend distributes ProViotic to over 1,000 retail stores including Whole Foods Canada.

Juice Press in Manhattan averages 14 bottles per store per week in sales – our best selling retail chain! We are in over 200

independent stores in California

In the UK we are in Planet Organic and Whole Foods

In Spain we are in over 500 independent stores.

In Austria, ProViotic is selling the in the Rauch stores. (Rauch is the largest Austrian juice manufacturer and the manufacturer of Red Bull worldwide)

In Bulgaria, we hold 20% market share.

We recently entered Switzerland

We are in 4000 CVS stores across USA

GLOBAL PROVIOTIC FOOTPRINT

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Pear Cider Ultra-Low-Alcohol ProViotic’s Multi-Spoke Marketing Strategy

Coordinates Six Strategic Programs

Multi-spoke

Marketing Strategy

In-store activities

Key Opinion Leaders

Coordination

Print Publications

Social Media Marketing

Ivy League Continuous

Research

Juice Press Cross

Marketing

Program Example of ProViotic Activity

Print Publications Featured in The Wall Street Journal, Oprah Magazine, The New Yorker, Gotham Magazine, etc.

In Store Activities Employee training, in-store booth, in store brochures, shelf-talkers

Ivy League Research Harvard Medical School findings create news opportunities. This year, we are investing $250,000 in our science program with the best Harvard researchers who will create interesting news opportunities if the scientific findings reconfirm our initial expectations.

Social Media ProViotic actively manages its Instagram and Facebook presence. We were recently featured by a prominent youtuber visiting Manhattan as a cool product in New York

Juice Press Cross Marketing ProViotic appears with Juice Press in major events such as the US Open Tennis Tournament, as well as stars such as Leonardo DiCaprio have become ProViotic consumers through Juice Press

Key Opinion Leaders ProViotic attends conferences such as the American Pediatrics Conference in Washington, where we present the advantages of ProViotic, we are also featured by some bloggers in the nutritional space

Sammi International Ltd.

+ 359 879 200 470 Tsvetomira Savova [email protected]

the purest probiotic on earth tm -...

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