疾病管制局

Strategy of Processing Complex Data

-TB Database As A Case Study

Pei-Ju Chin

Bioinformatics Core

Mycobacteria Reference Lab

Laboratory Research & Development Division

Center of Disease Control

Taipei, Taiwan, R.O.C.

疾病管制局

Flowchart of Genotype Analysis

疾病管制局

Experimental Data We Engaged

• Fingerprint

– RFLP, PFGE, PCR-RFLP

• Character

– VNTR, MIRU, Spoligotype

• Sequence

– 16s rDNA, SNP of MDR gene

• Matrix

– DNA Homology Matrix

• Cluster Analysis

– UPGMA, Ward, NJ….etc

• Dimensioning and Statistics

– PCA, MDS, SOM

• Identification

– Neural network, Basic Expert System

• Database Sharing Tool

• Server Package

疾病管制局

Fingerprint Type- RFLP

疾病管制局

Character Type- Spoligotype

疾病管制局

Character Type- VNTR-MIRU

疾病管制局

Contents In TB Genotype Database (Dec. 2002~Mar. 2005)

Experiment Present RFLP Spoligotype VNTR MIRU

Number 1197 2379 628 1098

疾病管制局

• Consulting IT Service Provider

– Advantage

• Without maintenance requirement

– Disadvantages

• Budget ?

• Compatibility ?

• Distributed System

– Advantage

• Flexibility

– Disadvantage

• Management is a difficult issue

Integrated Database: How ?

疾病管制局

Bi-Directional Data Sharing

疾病管制局

CDM Schema of TB InteGrated & Research Database (TIGR DB)

疾病管制局

疾病管制局

TB InteGrated & Research Database (TIGR DB)

• Laboratory-Oriented Database

• Focus on examination data, not epi data

– Bi-directional data sharing with EID division & TB division

• http://192.168.83.3/db1/