sphpp bioinformatics. 3 rd chr-16 workshop. miraflores de la sierra december, 10 th -11 th 2012...

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SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

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Page 1: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

SpHPP Bioinformatics.3rd CHr-16 Workshop. Miraflores de la SierraDecember, 10th-11th 2012 Alberto Medina

Page 2: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Summary

1. Logistics2. Activities and meetings3. Data Analysis pipeline

1. Shotgun2. MRM

4. Sharing data5. Viewing data6. Conclusions

Page 3: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Summary

1. Logistics2. Activities and meetings3. Data Analysis pipeline

1. Shotgun2. MRM

4. Sharing data5. Viewing data6. Conclusions

Page 4: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Logistics

• Logistics:• FTP. Public FTP for exchanging “regular” files. A new

system should be developed for high throughput instruments.• Mailing list. Different lists grouping by working

groups • Wiki space: http://community.uv.es/mediawiki/• Web site: http://hpp-sp.com• Google code site:

https://code.google.com/p/s-chpp/Webex teleconference

Page 5: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Website (http://hpp-sp.com)

Page 6: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Website. Consortium introduction

Page 7: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Website.WG introduction

Page 8: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Website. BD page

Page 9: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Website CHPP

Page 10: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

C-HPP Wiki

Page 11: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Summary

1. Logistics2. Activities and meetings3. Data Analysis pipeline

1. Shotgun2. MRM

4. Sharing data5. Viewing data6. Conclusions

Page 12: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Activities and metings

9/3rd: Follow-up teleconference.9/19th: Follow-up teleconference.10/3rd: Follow-up teleconference.10/17th: Follow-up teleconference.10/31st: Follow-up teleconference.11/21st: Follow-up teleconference.

Page 13: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Summary

1. Logistics2. Activities and meetings3. Data Analysis pipeline

1. Shotgun2. MRM

4. Sharing data5. Viewing data6. Conclusions

Page 14: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Summary

1. Logistics2. Activities and meetings3. Data Analysis pipeline

1. Shotgun2. MRM

4. Sharing data5. Viewing data6. Conclusions

Page 15: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Data Analysis Pipeline. Shotgun

Page 16: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Data Analysis Pipeline. Shotgun

Page 17: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

We are growing!

Page 18: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Main improvements

• Some bugs have been fixed

• Data Analysis could be directly done by end-users

• Sequest has been included.

• Protein Inference – Protein Grouping.

Page 19: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Data Analysis. Protein Grouping

Page 20: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Main improvements

• Some bugs have been fixed

• Data Analysis could be directly done by end-users

• Sequest has been included.

• Protein Inference – Protein Grouping.

• Mascot Server

• Curated experiments

Page 21: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Curated experiments

Page 22: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Summary

1. Logistics2. Activities and meetings3. Data Analysis pipeline

1. Shotgun2. MRM

4. Sharing data5. Viewing data6. Conclusions

Page 23: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Data Analysis. MRM

Page 24: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Data Analysis. MRM

Page 25: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Data Analysis. Proteotipic?

Page 26: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Summary

1. Logistics2. Activities and meetings3. Data Analysis pipeline

1. Shotgun2. MRM

4. Sharing data5. Viewing data6. Conclusions

Page 27: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Sharing Data

Page 28: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Sharing Data

Page 29: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Sharing Data

Page 30: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Summary

1. Logistics2. Activities and meetings3. Data Analysis pipeline

1. Shotgun2. MRM

4. Sharing data5. Viewing data6. Conclusions

Page 31: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Public Data

Page 32: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Viewing Data

Page 33: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Viewing Data

Page 34: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Viewing Data

Page 35: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Viewing other “data”

Page 36: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Summary

1. Logistics2. Activities and meetings3. Data Analysis pipeline

1. Shotgun2. MRM

4. Sharing data5. Viewing data6. Conclusions

Page 37: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Conclusions

• Website should be filled. WG description ASAP.

• Continuous activities and meetings.

• Data Analysis.

• Shotgun analysis is great!

• MRM DB

• Data submission pipeline. Sequest is “active”.

• Interaction with C-HPP and other chromosomes (TPB).

Page 38: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Acknowledges • CIMA:• Victor Segura• Fernando Corrales

• UCM:• Vital Vialas

• IIBB/CSIC:• Óscar Gallardo

• Ibermática• Aitor Moreno

• UPV:• Gorka Prieto• Kerman Aloria.• Jesús M. Arizmendi.

• UV:• Manuel S. Pino• Javi Órtiz

• CNB/CSIC:• Salvador Martínez

de Bartolomé.• Juan Pablo Albar

Page 39: SpHPP Bioinformatics. 3 rd CHr-16 Workshop. Miraflores de la Sierra December, 10 th -11 th 2012 Alberto Medina

Discussion

• Submission pipeline.

• Protein Inference. Resubmit the data.

• Sequence BDs.

• MRM queries.

• Logo and name.