Sockeye Salmon Captive Broodstock Program:

Spawning Review

Tier 1

Avoid population extinction using captive technology.

Conserve population genetic diversity.

Increase the number of individuals in the population.

Program goals

Tier 2

Move from a captive broodstock to an integrated broodstock and eventually de-list the population

Captive broodstock spawning principles:

- Equalize family size

- Factorial Spawning

- Maximize the number of breeders per generation

- Minimize inbreeding (relatedness)

Spawning Redfish Lake Sockeye Salmon

Pre-season…..Genetic samples are processed for the Age-3 captive broodstock.

Pre-season…..Ultrasound all Age-3 captive broodstock to determine maturation status and sex.

Pre-season…..Genotype returning anadromous sockeye to determine which fish to incorporate into the captive broodstock.

Pre-season…..Develop the spawning matrix.

Preparation:Determine which males are running.Determine which females are ready.Develop crosses.

Spawning Redfish Lake Sockeye Salmon

#3

3D9.1C2CF0 807D

ANH11 8169 3D9.1C2CF0 8613

3D9.1C2CFE 5311

ALT: A - 2533

N4: Yellow

#5

3D9.1C2CF0 807D

ANH11 99E5 3D9.1C2CFE BFAD

ANH11 0BC4

ALT: A - C356

N1: Orange

N2: Purple

D7: Purple

D7: White/Black

D7: Purple

D7: Dark Green

D8: Pink

Female Station:Strip green eggs into one gallon zip lockAdd oxygenPlace eggs in cooler

Spawning Redfish Lake Sockeye Salmon

Male Station:Identify males needed to cross with

female Collect milt in whirl-pakCheck motility of milt.Add oxygenPlace in cooler

Spawning Redfish Lake Sockeye Salmon

Fertilization Station:Split green eggs into three equal

groups. Fertilize eggs with milt from

appropriate male.20 minute iodophor disinfection.

Spawning Redfish Lake Sockeye Salmon

Incubation:Label incubator. Return to incubation area.

Spawning Redfish Lake Sockeye Salmon

The Next Generation:Equalize Family SizeSelect one or two eggs from each spawn

cross.

Spawning Redfish Lake Sockeye Salmon

Program Challenges:Spawn Timing – Anadromous vs Captive.Isolation between Anadromous and Captive

stocks.Low volumes of milt from captive males.Delay before fertilization occurs.Small number of sockeye spawned per day.

Questions

Spawning Redfish Lake Sockeye Salmon