Sockeye Salmon Captive Broodstock Program:
Spawning Review
Tier 1
Avoid population extinction using captive technology.
Conserve population genetic diversity.
Increase the number of individuals in the population.
Program goals
Tier 2
Move from a captive broodstock to an integrated broodstock and eventually de-list the population
Captive broodstock spawning principles:
- Equalize family size
- Factorial Spawning
- Maximize the number of breeders per generation
- Minimize inbreeding (relatedness)
Spawning Redfish Lake Sockeye Salmon
Pre-season…..Genetic samples are processed for the Age-3 captive broodstock.
Pre-season…..Ultrasound all Age-3 captive broodstock to determine maturation status and sex.
Pre-season…..Genotype returning anadromous sockeye to determine which fish to incorporate into the captive broodstock.
Pre-season…..Develop the spawning matrix.
Preparation:Determine which males are running.Determine which females are ready.Develop crosses.
Spawning Redfish Lake Sockeye Salmon
#3
3D9.1C2CF0 807D
ANH11 8169 3D9.1C2CF0 8613
3D9.1C2CFE 5311
ALT: A - 2533
N4: Yellow
#5
3D9.1C2CF0 807D
ANH11 99E5 3D9.1C2CFE BFAD
ANH11 0BC4
ALT: A - C356
N1: Orange
N2: Purple
D7: Purple
D7: White/Black
D7: Purple
D7: Dark Green
D8: Pink
Female Station:Strip green eggs into one gallon zip lockAdd oxygenPlace eggs in cooler
Spawning Redfish Lake Sockeye Salmon
Male Station:Identify males needed to cross with
female Collect milt in whirl-pakCheck motility of milt.Add oxygenPlace in cooler
Spawning Redfish Lake Sockeye Salmon
Fertilization Station:Split green eggs into three equal
groups. Fertilize eggs with milt from
appropriate male.20 minute iodophor disinfection.
Spawning Redfish Lake Sockeye Salmon
Incubation:Label incubator. Return to incubation area.
Spawning Redfish Lake Sockeye Salmon
The Next Generation:Equalize Family SizeSelect one or two eggs from each spawn
cross.
Spawning Redfish Lake Sockeye Salmon
Program Challenges:Spawn Timing – Anadromous vs Captive.Isolation between Anadromous and Captive
stocks.Low volumes of milt from captive males.Delay before fertilization occurs.Small number of sockeye spawned per day.
Questions
Spawning Redfish Lake Sockeye Salmon
