Redfish Lake Sockeye Salmon Captive Broodstock Rearing and Research

Project 199204000

Project Accomplishments 2001-2003

Resource Enhancement and Utilization Technologies Division

Manchester Research StationNorthwest Fisheries Science Center

National Marine Fisheries Service

National Oceanic and Atmospheric Administration

2725 Montlake Boulevard E.

Seattle, Washington 98112

Desmond Maynard & Thomas Flagg

Captive Broodstock Project Purpose

• Avoid catastrophic loss of ESA stock

• Amplify population to complement recovery efforts

ESA-Listed BroodstocksESA-Listed Broodstocks Manchester Research Station

•Redfish Lake sockeye salmon

•Grande Ronde spring chinook salmon

•Salmon River spring chinook salmon

•Idaho Department of Fish and Game•Idaho Office of Species Conservation•Oregon Department of Fish and Wildlife•Shoshone-Bannock Tribes•Nez Perce Tribes•Umatilla Confederated Tribes•US Fish and Wildlife Service•University of Idaho•Washington Department of Fish and Wildlife

Partners

•Northwest Power Planning Council•Columbia Basin Fish and Wildlife Authority•Bonneville Power Administration

NMFS Captive Broodstock Rearing Facilities

Burley Creek Captive Broodstock Facility

Manchester CaptiveBroodstock Facility

Seawater Rearing FacilitySeawater Rearing Facility

• Manchester Marine Laboratory

• 6,000 lpm processed seawater

• Sand and cartridge filtered to 5 u and then UV disinfected

• 400-m2 and 1,280-m2 broodstock rearing building

• Twenty 6.1-m circular tanks, six 4.1-m circular tanks, and six 1.8-m circular tanks

Freshwater Rearing Facility

• Burley Creek Hatchery

• 2,000 lpm 100C well water

• 613 m2 building

• Eleven 3.6 m circular tanks

• Fourteen 1.5 m circular tanks

• Incubation room

Relationship to Other Projects

Coordinated through SBSTOC

• IDFG sockeye captive broodstock (199107200)

• Shoshone-Bannock Tribe sockeye habitat (199107100)

• University of Idaho sockeye genetics (199009300)

• NMFS captive broodstock technology (199305600)

Rational and Significance to Regional Programs

• NMFS draft Snake River Salmon Recovery Plan

• NWPPC Fish and Wildlife Program objectives

• NMFS FCRPS Biological Opinion RPA

• Salmon Subbasin Summary needs and goals

2001-2003 Tasks

• Captive broodstock rearing

• Production of prespawning adults, eyed eggs, and smolts

• Refine rearing protocols

• Secure freshwater rearing facility (Burley Creek)

Captive BroodstocksCaptive Broodstocks Spawn Source Adults

F1

F2

F2

SUPPLEMENT

Hold FirstGeneration

Release SecondGeneration

First Generation Adults

ResearchResearch•genetics•physiology•growth•health•survival•behavior

Sockeye Captive Broodstock Sockeye Captive Broodstock Culture ConcernsCulture Concerns

• Survival– Culture parameters– Health status

• Morphological attributes– Adult size and physical appearance

• Physiological parameters– Reproductive development and spawn timing – Fecundity and egg viability– Post-release fitness

ESA broodstock reared to maturity under controlled conditions ...

Husbandry and Sampling

Growth Rate Modulation

0102030405060708090

100

0 2 4 6 8 10 12

Weight in May (g)

Mal

e m

atur

atio

n (%

)

BY 01

Exercise

• Experimental exercise protocols implemented (2003)

• Year two of six year evaluation

• Fecundity, egg viability, physiological parameters

Sockeye Captive Broodstock Sockeye Captive Broodstock Culture PerformanceCulture Performance

• Survival -- 50-80% egg-adult

• Size -- Equivalent to wild

• Egg Viability -- 60%+

Spawning

Breeding Plans for SockeyeBreeding Plans for Sockeye

Primary tools used:

• Pedigree information

• Life history information (year-class)

• Origin (hatchery, wild, residual)

• Parentage assignment (DNA)

(PIT tags)

NMFS Redfish Lake sockeye salmon egg production

Spawn Year Number

1994 48,000

1995 0

1996 412,500

1997 168,165

1998 47,533

1999 65,400

2000 96,698

2001 90,859

2002 60,516

2003 139,359

TOTAL 1,129,030

NMFS Redfish Lake sockeye salmon captive broodstock distribution

• Prespawning adults; n = 660 released

• Eyed eggs to IDFG; n = ~810K

• Eyed eggs to Bonneville; n = ~250K IDFG

Determine the effectiveness of smolt releases for restoring anadromous runs of Snake

River sockeye salmon

• Coded Wire tag all sockeye reared at Bonneville Hatchery

• PIT tag a subsample of sockeye salmon reared at Bonneville Hatchery

• Release Bonneville Hatchery Fish into Stanley Basin Lakes

• Compare the survival of these fish to IDFG data on prespawning adults, eyed eggs, and presmolts

Release Strategies EvaluationsRelease Strategies Evaluations

Outmigrant monitoring:PIT tags and unique fin clips

Redfish Lake Creek weir

Mainstem dams

20001950 1960 1970 1980 1990

100

50

150

200

300

250

350

400

450

500

550

600

6000

3000

1000

4,361

Adult Return Year

Nu

mb

er o

f F

ish Adult Sockeye Salmon ReturnsAdult Sockeye Salmon Returns

To Redfish Lake 1954 - 2002

16wild/natural

adults

320+hatchery-produced

adults

Redfish Sockeye Captive Broodstock Returns to Redfish Sockeye Captive Broodstock Returns to the Stanley Basin, 1999-2002the Stanley Basin, 1999-2002

Mark Outmigrate Returns SAR

Unmarked 6,000 20 0.33%

Ad Clip 109,000 69 0.06%

Ad-LVClip, CWT

67,000 199 0.30%

• Hatchery enhanced natural production (Unmarked) • Presmolts to overwinter in lake (Ad Clipped)• Smolts (Ad-LV clipped, CWT)

Redfish Sockeye Captive Broodstock Returns to Redfish Sockeye Captive Broodstock Returns to the Stanley Basin, 1999-2002the Stanley Basin, 1999-2002

Mark Release Outmigrate Returns SARCB

femalesspawned

CB/R

Unmarked 240adult/

210,700eggs

6,000 20 0.33% 215 10.7 : 1

Ad Clip 437,800 109,000 69 0.06% 218 3.2 : 1

Ad-LVClip, CWT

67,000 67,000 199 0.30% 77 1 : 2.6

• Hatchery enhanced natural production (Unmarked) • Presmolts to overwinter in lake (Ad Clipped)• Smolts (Ad-LV clipped, CWT)

Task 2 -- Secure Burley Creek Facility

• Current status– Approved for FY2004

– NEPA process complete

– NOAA/BPA MOA for purchase completed

– BPA and NOAA Fisheries approval

Questions

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