Sneha Krishnamurthy, PhD

Instructor, Department of Biology

Western Kentucky University

Bowling Green, KY-42101

E-mail: sneha.murthy@wku.edu

Work Experience

Jan 2015~present Instructor, Western Kentucky University, USA

• Teaching load includes General Biology (192 students/class), Anatomy and physiology lecture and lab s (24

-160 students/session), Microbiology Labs (24 students/session) for majors and non-majors; Average student

rating is 4.17 against the Department average 4.2

• Traditional clickers cost $15/student. I used a card reader for monitoring attendance at no cost to the student.

Observed 45% increase in student attendance

• Interfaced with engineering department to develop 3D models to improve student engagement

Nov 2012~Dec 2014 Post-doctoral fellow, Western Kentucky University, USA

Managed an NSF grant worth $375,413.00 and published 5 papers in peer reviewed scientific journals like

Scientific Reports (Nature publishing group) and Genomic Data. The project deciphered novel mechanistic

aspects of gold ion uptake in live plants

Guided 2 students in their research theses currently placed in University of Louisville and Monsanto, St.

Louis

Invited speaker at National Institute of plant genomic research (India)

Reviewed over 10 papers in various scientific journals

Feb 2012~Oct 2012 Assistant research professor, Jeonbuk National university, South Korea

The patent entitled "Method for preparing metal nanoparticles using biomass (10-1169523)" was licensed

out to Nanosolution Co., LTD at a price of US$ 8670; High-performance biomass-chitosan composite

fibers used for a trial run in the Ulsan factory of Samsung-BP Chemicals, Co., LTD to confirm their

superior performance in the real scale to commercial resins

Mentored 2 students in their masters theses, one student currently a part of Samsung-BP chemicals

Published over 5 papers in peer reviewed scientific journals like ACS Sustainable Chemistry &

Engineering and Chemical Engineering Journal

July 2011~Feb 2012 Postdoctoral fellow, Jeonbuk National University, Jeonju, South Korea

Collaborated with department of molecular biology and chemical engineering and University College

London (United Kingdom) to produce over 3 publications

Transferred trade secrets (the know-how) in design and manufacture of biosorbents for water softening

have been transferred to Coway Co., LTD at a price of US$ 130056

May 2007-2008 Research Trainee Madras Biotech, Chennai, Tamilnadu, India.

Manufactured and packaged DNA & protein Markers & PCR products

Planned and organized workshops in 5 city college for Biology Majors, mentored 7 interns

Provided hands on training for over 100 students, on techniques in molecular biology

Teaching Experience

Courses I currently teach:

General Biology (Biol 113)- Prokaryotes/Eukaryotes, Basic cell structure and function, Cell division, Cell metabolism, respiration and

photosynthesis, Basic introduction to the origins and diversity of life forms such as viruses, archaea, bacteria, Protista diversity and fungi.

DNA structure and function, General principles of genetics, chromosomes, genes and practical applications of DNA technology, Ecology

and ecosystems, conservation biology.

Anatomy and Physiology (Biol 131)-Introduction to Body Regions and Directional Terms, Histology: Epithelium, Muscle, Nervous Tissue,

Connective Tissue (Cartilage, Adipose, Blood and Osseous tissues), Muscles and Bones of the body, Cardiovascular System, Nervous

System: Reflex Arc, Special Senses.

Microbiology lab for health sciences (Biol 208)– Lab safety, handling and examining the cultures, Pure culture techniques, Microscopy,

Wet-Mount preparation, Staining techniques (simple, gram, endospore, acid-fast, fungal staining), Biochemical and enzymatic reactions,

Rapid methods of identification, Media for isolation of bacteria and fungi, Antibiotics, susceptibility testing, MIC, Urine culture techniques

(Dip-stick test, qualitative and quantitative), Isolation of a culture from throat, stool studies and culture, serological identification of

microbes and patient antibodies, Antibody titer. The lab mainly covers diseases, islation and identification of clinically relevant organisms

like Streptococci sp., Staphylococcus sp., Enterococcus sp., Klebsiella sp., Hemophilus sp., Corenebacteria sp., Bordetella sp. Neisseria

sp., Mycobacterium sp., Clostridium sp., Enterobactericiae, spirochetes.

Other subjects taught

Microbiology (Majors and non-majors) while in Chonbuk National University, South Korea. (Aust 2008-2012)

Introductory cell and molecular biology while in Madras Biotech., India. (May 2007-2008)

Certificate course in Techniques in cell and molecular biology for Biology majors while in Madras Biotech., India. (May 2007 -2008)

Organized workshops in certificate course certificate course in Techniques in cell and molecular biology for Biology majors

Inter-lab Research Collaborations

University College London, United Kingdom In situ time-resolved X-ray absorption spectroscopy and small angle x-ray

scattering measurements on crystal growth of gold nanoparticles use plant

extracts.

Michigan Technological University, USA Proteomic analysis of in planta synthesis of nanoparticles using Arabidopsis.

National Research Center on Plant Biotechnology Global genomic profiling of Arabidopsis and identification of Fe transporter

genes in response to chloroauric acid.

Jeonbuk National University, South Korea Biosorption of precious metals from industrial waste water; bio-inspired

synthesis of precious metal nanoparticles.

Other Achievements

Article featured in the top 25 list of most downloaded articles for Colloids and Surfaces B: Biointerfaces (October 2009 – September

2010 academic year): Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its

bactericidal activity.

h-index of 10 and 652 citations since 2010.

Outreach activities

Faculty Mentor for Anatomy and Physiology (Biol 131) Peer Assisted Study Sessions (Current)

Assisted rat-dissection workshops for Warren County Schools (April 2016)

Made test for Anatomy and Physiology Olympiad (April 2016)

Other skills

Computer Skills:

Blackboard Learn System software, Polleverywhere

Highly experienced with Office applications, Photoshop software, OriginLab, Sigma plot and Image J Pro, Endnote X6,

Other Skills:

Writing research articles, project proposals and reports, oral presentations and mentoring skills

Educational qualifications

Ph.D. Bioprocess Engineering, Chonbuk national University, South Korea August 2011

M.Sc. Biotechnology, Bharathiar University, India May 2007

B.Sc. Biotechnology, P.S.G College of Arts and Science May 2005

Research Experience

1. Development of bio-inspired protocols for fabrication of metal nanoparticles

2. Toxicity of chemically and biologically synthesized nanoparticles in microbes and plants.

3. Screening of marine bacteria for production of glycolipids and production of glycolipids – a low molecular weight biosurfactant,

from Halomonas hydrothermalis

4. In-situ SAXS and XAS analysis of biogenic gold nanoparticles at Diamond synchrotron(UK) and Euoropean Synchrotron

radiation facilities (France)

5. Research and manufacture of DNA and Protein molecular standards -gene cloning and Protein expression

6. Marine Waste Management by Marine Meat Agar from Marine waste-collection of marine waste from shore of Mumbai (dead

fish, shrimp, crab etc.) was cleaned. Meat Agar prepared and marine isolates grown on meat agar with glucose as source of carbon

7. Chitinase Producing Organisms-shells of dead marine animals were collected and chitin was purified by using concentrated HCl.

The growth of marine organisms on chitin agar was compared to that on pure practical grade chitin. Efficient isolates were obtained

by measuring the diameter of clearance zone formed as a result of chitinase production by marine organisms.

Methodological expertise

1. UV-Vis-NIR Spectroscopy, Inductively Coupled Plasma Atomic Emission Spectroscopy (ICPS-7500 Shimadzu), Fourier

Transform Infrared Spectroscopy (FTIR)

2. Real time PCR and Microarray analysis

3. Scanning Electron Microscopy (SEM/FESEM), Transmission Electron Microscopy (TEM)

4. General microbiological techniques

5. Isolation of DNA, purification techniques, DNA quantification, PCR, Transformation and cloning techniques

6. Protein isolation, protein induction and production in E.coli, protein purification

7. Bioinformatics analysis of genome, primer designing

8. X-ray diffraction, X-ray photon spectroscopy, familiar with synchrotron based techniques (X-ray absorption spectroscopy and

small and scattering)

9. Basic animal and plant cell culture techniques

Patents

1. Chiotosan -Biomass composite and preparation method thereof

SepCh/20/2012, 10-2012-0104666, Industrial Cooperation Foundation Chonbuk National University, Yun Yeoung Sang, Kwak In

Seob, Bae Min A, Won Sung Wook, Choi Sun Beom,Kim Sok, Mao Juan, Song Myung Hee, Park Ji Yeong, Lee Shi Yn, Sneha

Krishna Murthy, Pham Thi Phuong Thuy, Park Seong In, Han Do Hyung, Jeong Yong Sik (patent application)

2. Method for preparing metal nanoparticles using biomass

Jul/23/2012, 10-1169523, Industrial Cooperation Foundation Chonbuk National University, Yun Yeoung Sang, Kwak In Seob, Park

Ji Yeong, Won Sung Wook, Choi Sun Beom,Kim Sok, Mao Juan, Song Myung Hee, Bae Min A, Lee Shi Yn, Sneha Krishna Murthy,

Pham Thi Phuong Thuy, Park Seong In

3. Method for preparing metal nanoparticles using plant extracts

May/02/2012, 10-1144416, Industrial Cooperation Foundation Chonbuk National University, Yun Yeoung Sang, Kwak In Seob, Park

Ji Yeong, Won Sung Wook, Choi Sun Beom,Kim Sok, Mao Juan, Song Myung Hee, Bae Min A, Lee Shi Yn, Sneha Krishna Murthy,

Pham Thi Phuong Thuy, Park Seong In (patent application)

4. Metal-ionic liquid hybrid thin film and method for preparing the same

Dec/26/2011, 10-2011-0142283, Industrial Cooperation Foundation Chonbuk National University, Yun Yeoung Sang, Sneha Krishna

Murthy, Ko Yulim, Gopinathan SANKAR (patent application)

5. Hydrogel having reducing agents and Method for preparing the same

Dec/20/2011, 10-2011-0138164, Industrial Cooperation Foundation Chonbuk National University, Yun Yeoung Sang, Park Seong In,

Bae Min A, Kwak In Seob, Kim Sok, Sneha Krishna Murthy, Pham Thi Phuong Thuy, Song Myung Hee, Asiah NuSaibah, Ko

Yulim, Lim Areum (patent application)

6. Polymer gel containing metal extractant, Method for manufacturing the same and Method for recovering precious metal

using the same

Nov/18/2011, 10-2011-0120763, Industrial Cooperation Foundation Chonbuk National University, Yun Yeoung Sang, Park Seong In,

Bae Min A, Kwak In Seob, Kim Sok, Sneha Krishna Murthy, Pham Thi Phuong Thuy, Song Myung Hee, Asiah NuSaibah, Ko

Yulim, Lim Areum (patent application)

7. Method for biomaterial-coated polymer gel and polymer gel thereof

Nov/08/2010, 10-2010-0110492, Industrial Cooperation Foundation Chonbuk National University, Yun Yeoung Sang, Park Seong In,

Bae Min A, Kwak In Seob, Won Sung Wook, Choi Sun Beom, Kim Sok, Park Ji Yeong, Lee Shi Yn, Sneha Krishna Murthy, Pham

Thi Phuong Thuy, Song Myung Hee (patent application)

8. Chiotosan -Biomass composite and preparation method thereof

Jul/05/2010, 10-2010-0064456, Industrial Cooperation Foundation Chonbuk National University, Yun Yeoung Sang, Kwak In Seob,

Park Ji Yeong, Won Sung Wook, Choi Sun Beom, Kim Sok, Mao Juan, Song Myung Hee, Bae Min A, Lee Shi Yn, Sneha Krishna

Murthy, Pham Thi Phuong Thuy, Park Seong In, Han Dohyeong, Jeong Yongsik (patent application)

Publications in peer reviewed journals

1. Tiwari M, Krishnamurthy S, Shukla D, Kiiskila J, Jain A, Datta R, Sharma N, Sahi SV. Comparative transcriptome and

proteome analysis to reveal the biosynthesis of gold nanoparticles in Arabidopsis. Scientific Reports, 2016. 6: p. 21733.

2. Lee SY, Krishnamurthy S, Cho CW, Yun Y-S, Biosynthesis of Gold Nanoparticles Using Ocimum sanctum Extracts by

Solvents with Different Polarity. ACS Sustainable Chemistry & Engineering, 2016. 4(5): p. 26512659.

3. Sneha K, Yn LS, Yeoung-Sang Y. Optimization Studies of Conditions for Biological Synthesis of AuNPs in Various Shapes

Using Plant Extract (Ocimum sanctum). J Nanosci Nanotechno. 2015;15(1):326-9.

4. Shukla D, Krishnamurthy S, Sahi SV. Microarray analysis of Arabidopsis under gold exposure to identify putative genes

involved in the synthesis of gold nanoparticles (AuNPs). Genomics Data. 2015;3:100-2.

5. Ko Y-L, Krishnamurthy S, Yun Y-S. Facile Synthesis of Monodisperse Pt and Pd Nanoparticles Using Antioxidants. J Nanosci

Nanotechno. 2015;15(1):412-7.

6. Krishnamurthy S, Esterle A, Sharma NC, Sahi SV. Yucca-derived synthesis of gold nanomaterial and their catalytic potential.

Nanoscale Res Lett. 2014 Nov 23;9. PubMed PMID: WOS:000346168900001. English.

7. Jain A, Sinilal B, Starnes DL, Sanagala R, Krishnamurthy S, Sahi SV. Role of Fe-responsive genes in bioreduction and

transport of ionic gold to roots of Arabidopsis thaliana during synthesis of gold nanoparticles. Plant Physiology and

Biochemistry. 2014; 84:189-196.

8. Shukla D, Krishnamurthy S, Sahi SV. Genome wide transcriptome analysis reveals ABA mediated response in Arabidopsis

during gold (AuCl− 4) treatment. Frontiers in plant science. 2014; 5:652.

9. Sathishkumar M, Sneha K, Yun YS. Green fabrication of zirconia nano-chains using novel Curcuma longa tuber extract. Mater

Lett. 2013 May 1;98:242-5. PubMed PMID: WOS:000320078300064. English.

10. Krishnamurthy S, Yun Y-S. Recovery of microbially synthesized gold nanoparticles using sodium citrate and detergents.

Chemical Engineering Journal. 2013;214:253-61.

11. Sneha K, Sathishkumar M, Lee SY, Bae MA, Yun YS. Biosynthesis of Au Nanoparticles Using Cumin Seed Powder Extract. J

Nanosci Nanotechno. 2011 Feb;11(2):1811-4. PubMed PMID: WOS:000287167900181. English.

12. Mao JA, Kwak IS, Sathishkumar M, Sneha K, Yun YS. Preparation of PEI-coated bacterial biosorbent in water solution:

Optimization of manufacturing conditions using response surface methodology. Bioresource Technology. 2011 Jan;102(2):1462-

7. PubMed PMID: WOS:000286782700140. English.

13. Sneha K, Sathishkumar M, Mao J, Kwak IS, Yun YS. Corynebacterium glutamicum-mediated crystallization of silver ions

through sorption and reduction processes. Chemical Engineering Journal. 2010 Sep 1;162(3):989-96. PubMed PMID:

WOS:000282137500015. English.

14. Sneha K, Sathishkumar M, Kim S, Yun YS. Counter ions and temperature incorporated tailoring of biogenic gold nanoparticles.

Process Biochem. 2010 Sep;45(9):1450-8. PubMed PMID: WOS:000281091700003. English.

15. Sathishkumar M, Sneha K, Yun YS. Immobilization of silver nanoparticles synthesized using Curcuma longa tuber powder and

extract on cotton cloth for bactericidal activity. Bioresource Technology. 2010 Oct;101(20):7958-65. PubMed PMID:

WOS:000280340300038. English.

16. Kwak IS, Bae MA, Won SW, Mao J, Sneha K, Park J, et al. Sequential process of sorption and incineration for recovery of gold

from cyanide solutions: Comparison of ion exchange resin, activated carbon and biosorbent. Chemical Engineering Journal.

2010 Dec 1;165(2):440-6. PubMed PMID: WOS:000285369600008. English.

17. Sathishkumar M, Sneha K, Yun Y. Palladium nanocrystal synthesis using Curcuma longa tuber extract. Int J Mater Sci.

2009;4:11-7.

18. Sathishkumar M, Sneha K, Won SW, Cho CW, Kim S, Yun YS. Cinnamon zeylanicum bark extract and powder mediated green

synthesis of nano-crystalline silver particles and its bactericidal activity. Colloid Surface B. 2009 Oct 15;73(2):332-8. PubMed

PMID: WOS:000269598900025. English.

19. Sathishkumar M, Sneha K, Kwak IS, Mao J, Tripathy SJ, Yun YS. Phyto-crystallization of palladium through reduction process

using Cinnamom zeylanicum bark extract. Journal of Hazardous Materials. 2009 Nov 15;171(1-3):400-4. PubMed PMID:

WOS:000271165100050. English.

Papers in progress

1. K. Sneha, Y.S. Yun, Ionic Liquid EMIM acetate: A stepping stone to recovery of biogenic nanoparticles from microbial biomass.

(Submitted)

2. K. Sneha, G.Portale, S. Nikitenko, Wim Bras, Yeoung-Sang Yun and Gopinathan Sankar On the Nature of Biogenic Gold Nano Particle

formation. (Submitted)

3. K. Sneha, Y.S. Lee, M. Sathishkumar, Y.-S. Yun, Facile fabrication of crystalline noble metal nanoparticles using novel marine isolates

and application of their microbial consortium for synthesis of nanoparticles from synthetic wastewater. (Submitted)

4. K. Sneha, G.Sankar, Y.S. Yun, Room temperature deposition of Au nanoparticles-ionic liquid hybrid film (<10 nm), on Si (111)

substrate.

5. K. Sneha, G.Sankar, Y.S. Yun, A combined XRD, XPS and XAS analysis for the effects of Bromide and Iodide ions on nanoparticles

synthesis.

Conference attended (oral presentations)

1. K. Sneha, Y.L. Ko, Y.-S. Yun, Green technology for fabrication of metal nanoparticles. The Korean Society of Clean technology

Conference, Jeonju, Korea. (2011.11.24~25)

2. K. Sneha, S.Y. Lee, J.Y. Park, Y.-S. Yun, Development of bioinspired protocols for fabrication of metal nanoparticles. NANO2010

India, Erode, India (2010.12.13~16).

3. K. Sneha, S.Y. Lee, Y.-S. Yun, Microbial Synthesis of metal nanoparticles: Screening, optimization and recovery. Korean Society of

Environmental Engineers Spring Conference, Jeju, Korea (2010.5.6~7)

4. K.Sneha, M. Sathishkumar, S. Kim, Y.-S. Yun, Halides, counter ions and temperature induced tailoring of Piper betle extract

biosynthesized gold nanoparticles, International Conference on materials for Advanced Technologies, Singapore

(2009.6.28~2009.7.3)

5. K. Sneha, M. Sathishkumar, S. Kim, J. Mao, Y.-S. Yun, Corynebacterium glutamicum mediated crystallization of silver ions through

sorption and reduction process. The spring Conference of the Korean Society of Biotechnology and Bioengineering, POSCO

International center, Pohang, Korea (2009.4.9-11)

Poster Presentations

1. Deshpande A, Krishnamurthy S, Sahi SV, Comparison of antibacterial effects between biologically and chemically synthesized

silver nanoparticles, National conference on ungraduated research. (2014.04.03~05)

2. Fleischmann P, Shukla D, Bhaskaran S, Murthy S, Sahi SV, Study of morphophysiological adaptations in alfalfa under high

phosphate regime. American Society of Agronomy (2014.02.0 2)

3. K. Sneha, M. Sathishkumar, Y.-S. Yun. Piper betle mediated synthesis of gold nanoparticles. 6th Environmental Biotechnology

symposium, Kangwon-Do, Korea (2009.2.10~12)

4. K. Sneha, S.Y. Lee, Y.-S. Yun, Bacteria-mediated synthesis of noble metal nanoparticles, Nano Korea, Kintex, Korea (2009.8.26~28)

5. K. Sneha, S.Y. Lee, Y.-S. Yun., Biosynthesis of gold nanoparticles using Cumin seed powder extract. International conference on

nanoscience and nanotechnology, Gwanju, Korea (2009.11.05~06)

6. K. Sneha, M. Sathishkumar, S. Kim, Y.-S. Yun. Plant Extract Assisted Reduction of Platinum Ions to Nanoparticles. Asia Pacific

Biochemical Engineering Conference, Kobe, Japan (2009.11.24~28)

7. K. Sneha, Y.-S. Yun, Recovery of microbially synthesized gold nanoparticles using sodium citrate and detergents. Nano Korea,

Kintex, Korea (2010.8.17~20)

8. K. Sneha, Y.-S. Yun, Tapping of marine resources for biological synthesis of metal nanoparticles. The 3rd International Conference

on Multi-Functional Materials and structures, Jeonju, Korea (2010.09.14~18)

9. K. Sneha, S.Y. Lee, J.Y. Park, Y.-S. Yun, Intracellular synthesis of crystalline gold using live bacterium and its purification. Korean

Chemical Engineering Conference 2010 KICHE, Daejon, Korea (2010.10.10~20)

10. K. Sneha, J.Y. Park, Y.S. Lee, Y.-S. Yun, Gold Nanoparticle Synthesis by Marine Bacterium Jeotgalibacillus sp. International

Conference on NanoScience and Nanotechnology, Gwangju, Korea (2010.118~9)

11. K. Sneha, S.Y. Lee, J.Y. Park, Y.-S. Yun, Intracellular synthesis of crystalline gold using live bacterium. Advanced Environmental

Biotechnology Symposium, Muju, Korea (2011.02.15)

12. M. Sathishkumar, S.W. Won, K. Sneha, Y.-S. Yun. Non-chemical nanocrystalline silver particles synthesis using Curcuma longa

tuber extract and its immobilization on cotton cloth for bactericidal activity. 2008 Fall Korean Society of Biotechnology and

Bioengineering meeting and international Symposium. Jeju ICC, Korea (2008.10.06~07)

13. M. Sathishkumar, K. Sneha, S.W. Won, Y.-S. Yun. Cinnamom zeylanicum bark extract assisted biosynthesis of platinum

nanoparticles. International conference on nanoscience and nanotechnology, Gwanju, Korea (2008.11.06~07)

14. M. Sathishkumar, S.W. Won, K. Sneha, Y.-S. Yun. Natural synthesis of zirconia nano-chains using novel Curcuma longa tuber

extract. International conference on nanoscience and nanotechnology, Gwanju, Korea (2008.11.06~07)

15. S.Y. Lee, K. Sneha, M.-H. Song, Y.-S. Yun, Osmium sanctum mediated biosynthesis of gold nanoparticles, Nano Korea, Kintex,

Korea (2009.8.26~28)

16. S.Y. Lee, K. Sneha, Y.-S. Yun Investigation on Plant mediated synthesis of Au Nanoparticles. International conference on

nanoscience and nanotechnology, Gwangju, Korea. (2009.11.0 5~06)

17. S.Y. Lee, K. Sneha, Kwak, I.-S., Yun, Y.-S., Mechanistic aspect of biogenic synthesis of gold nanoparticles, Asia Pacific Biochemical

Engineering Conference, Kobe, Japan (2009.11.24~28)

18. S.Y. Lee, K. Sneha, Y.-S. Yun, Partial Identification of Tulsi Plant biochemicals responsible for Gold Nanoparticles formation. 2010

Korean Society of Environmental Engineers Spring Conference, Jeju, Korea (2010.5.6~7).

REVIEWER EXPERIENCE

Reviewed over 10 journal papers for various scientific journals.