tt-

I --I

;·L

Q,J>-

f-..

;-

~I! +-1,

.,fde~fQ)-/~Q71~

,)aof);:),'J1.;/P!I;:;

,... j..fgC-Xlcl

JpEIC-~cI~I"!"/--~'WI.••••'f";~I~,

-r-r,II,.,11.1'-11.1i''I--Jv:V'11"1b5..--

J~t.c0~J4-?h~J.;•...1,.I'Ir'''"'J- L-

~.J....~~"Irw"JIff"~J/I-~,I~'"i$'.:I.,iJ,.,

Ph~~O~II? -....,....,.. l<1d--yc.')1y~"Id.'1~r-,IF'/s:'f"SI.,~1-10

v=••.Il:XJ.;tl.Ji

•rJ',

:yQ£(-~eC7teJ/...•It-"",•...•Iff.l/Vio'PI'Sv.J}PJ:>1;]':J:i r.,

~../r.hl:,,~J~~'lSc1t(,toS"Go;)<)r-tJ.,'5O.$j1ft-00~-er-

1'jf)('~~'1"0r'!sS.)/-"V'j.fIIW.Y~.fd-

,.•..,S":>O~-e.')~-er Jri,l s,1SS1,}-,~1",;;.4co

7~.;)j:.')f-"/~

,~IAI.0.1ej.--00;)Ie,~ ..-- •J,f,rp"~.,;;D~'wf(),J..I~S'r,j#.:OJ)Q'rSJ1Iei~O-.. ,,

...II

'bt.)r/,l.If'PJI'iI15<;)~~b}p-Id~l~l--

".-.G)/,.1101~I/[AIJ~)c ,"

I.-,.-{ ,1<71,110w\:0rl'~7\M"-;--'7..J.I,'Jr..•••,I:l0-l'~~) r-r.:7;-

"

I

SI/I'\.f:Jo1'-I-LfS--_..,,-

'.....•...,I• ~

Y1In;..J.:ltd\IIJde'~~[)Oo~vJe.:;)C)va..,!.IJ

J0.:>r:JJS1-A6}IlIJ1..'()...

•II,/Ot'II'"<;;>11>1/1

Dl-'l.:>]AIa>1-"((se•YII....•"'0 'V~_w.,)110סi.

jl~-0")s-v-''''','~1::.",.In1~~sJ/tJ~III.'~Jf1)1 7'

~-0'-'rl""~:.,.""IY

:7Ci)WI1M'5Li'SIr:~1h'j~H~P<i vr'<;7I

l"bJ.NVl

~-iOJ)l!'JI_.I~~I~{.d'"0';'SGIfUIAJ::JJI.;

VI~10«--11/,i"1p-ell(,fa~JI/1~.,;D~J~9/}-J"'~IfA~/v .;0

-J

~"'IJ.~'~IJ(~~S-}J;;ILf'rlO~...,n.j.SIt;A:::I"//hi..,lA,~St>4 ..'"-

1\

-t \11,),Jstt.r-rt:7I~""J:::).1"VIJs,,-;.~~'"S"'-<pr-/-iJ=>~~II/)) 'Vv

/~8-n-v:rD II

,

z)01

I

I\

I

I

171/~

I"OII.I~"l/~~;I.-;VIH'~~TriP~1Q)ll1Q!~---+----+----t-----tpT.i.;.~J=.)~)r'I(Af./O,,'~-r'<.7~V~..•SI~110'1'"~()~J/-;

~".1;'..:1))1GII"'.'\..:ycy...'51~ll,pr'·:ffc;5'/.,,-y~~I)I~--+---+---+--+----+--+--+

I

IC-Y

1\I'r.,):::,

-

-

+

~-I--+--

£DCII1

I I

I

~I ---+-~--.•.--+-

,,

~ +-r--

-~~--

I--I--~~Iff174):W1.1y)('~J

r'IUPI/}t11/,AblA -../

1IIII)j....-:cUlt~OI-I

O(J'.'.J•..~~1.)(.~~SOIjJo(~AI1:)/)fX}:::)J-/.---<----

I,)J,411,~°1

~JIJqr,u~~s<d.J'f--,-

jr---"'/..-.I-

,,H.I"~W-IXl.~L~IAF"<9/-S:;}JIAtd+}C):>0'~~~

I\JbU,'f/--f---..--+-

-><bI

'TU-,'0'I '----i

~-,.,

#5,~jA"0I(j)fA:Y/:7b.l~d.';>~~C;,~/-/-UQ'.:J-

..i

.)10[VIAl~v••'S/IqI'In~..l~J&Vc-}::;6V/.A)102)~~

I 7-fOr(/-

t;J.J.().\Jdy-I-G'IS---I---,.•.- :

I ---+

--+--

I~r-l.(anrtIlAACuy I

-+-~-~' ::...V:JIlJ.~r)(j1/let.f-ISc;nWl1M••I/'"(.LAa'rUl'rI-5y--fb J

rei-IPM''/1(;i)W':cI,;I/~!IV;//->,bJ)J~S,dJ"I.]-

r=:i100 , CJHf-",)s\,:;u=:0I~'10/JJ'rI-.J~-qUAIJ-I-I£)'O"'d~

-I--------t-}:::)~

If"VI.tilJ.,0/')).nG)-11/cP):JAoJ'II

~rI'ft:.1/'fo,1/1

+-- v·•••.'J I\.•J

+""1·sleY)pef1/bl?I:JJ-,I:../Qu(i)),QAJ.."~/CCV/~"1IjOI

-j.i<-----

!it'-:lbflWtl''cbrq-I-2:.)v'-"7Sdf~/'Jf.,(..fC:>~0~'HIl

•cr::J,O(PI/~rl-;OJv'~cS>f''''''1:1'IV/~'.•..~

fr~}Isc;I$C~Joj.r-

~y4Of-rsVltn~sdJPel~'HlN\(I~WiP=,II--+--r- ("J

0(jllft:)~J1/-,Ir--+----~

~IJ-..,l-IC~1.J.16"<:;>0~\~/I••••-:1.Ip--lIi~n-J..aJ .'/'f-.....

Ip·Cvlfc~-:5iq,(.~V~IP/d..J-f-yc)J.~5~O~a(JJIA1-

....,,r;}o5S"<PI!..,'(jJ0'S'5(1)1to

-+--+--!

.?<;)....•~/'>'>.Jortt+""0fV1~11/....••II!.-I()QIIC(-Mj'bj'?:

f---,!

r--~-f----.-+-

)II11- r"III.st1">-~!

Ort,~~/'j,IID.,Ij-l1~(i(l()~ CT~r

IdJoc+--+--+-f....,

,~Q~~~!7~'aj'CfebSSG';'~""/~/"DVtA.:sI)l:>.~ --I---+-."

I,I...•

1-Y(<:(lg);i14"Jf,!7(IlAJS111JP.fxrJ(~_lIltlpn(JV(i_,,tb I

-f~Jo",J.}"tJj-"'.IrJ.--;)/1OLt~d~?::J.'jI"01ljj~,

1+---+---~-t

-.

IlAA.~-I-

(h/~y-:7r(,adPI~(~~/9eaq'1o..f...

0yv- - I~l:l.)..')jJ'.?I.1,/11.AQIJ~ij;n-+----

~IU'\(5- ~

rY>;.,i:>;/,JJ.>ut~aid'11r(: c;;;~:J~(~:\.~)'Yl"'\--:'\.]-)QIS'ts. '1'"":..J"/..I

Jt~-SitiI

.J I, I

I171)C1I

I

-+I

T:=-t-:-~~--

f1- ---IIit-t-

,t

-:1-1--

.(')1 -h

+-t-I----

]~ -.-~LI---~~

.ri:)JQ~~

L::.1~liDIIA~,. ~t'}sjI

,~-\..0- i

l -J-

~-It.~1£1t:J,~Jbl?lfi~CDS/.s r-......;

I

~~?"F/v4'iia'l-lhI.'Lu):,l...,-::>~4051L +

I..., +t f.--.l(w<i

17",~Ol'ilO'\j1;+51-T 5~.JrxJiWfJQI~t-

t---+.- J1.515~t

-+-

I~~,ItJ,t1.I.•..•II•"..1II11/ll'fb~'"It'd\ov)..1.5:; ~1/T

--<

I.I~-oj

---''0..5/a~~(r;J7~"'~LIIJ; +-~~'-----+~.-~+.~~ /IQ~-L--

/~~VJW~~z,~/lJei</.....~p~>------+

AA1/,/~PM'r;I.,Jc~Ii/~JJJJ::.Wc1J5~ 7

=l+

.-I-+--I-

IX'aifI--+-

- ),-~1IJ17>pc.DlM~L~~-

I

1._

'--.~cis""+-t-----'--

QL(.:2,-&~~-5~'-'(~I- .-'P-S~rlt.1'rIllA~Q~Q,j

'ljb1-I~}L-i ---tf-----'->------<

L,-,!hiJz-!J2_ Gh

IIgS~.- iI.- i

+..•.

i

I~__~'-/IS/~).1fill-~hfJ,

L!h~'Q~,~

,V.!Q.,I~

tGl""h\6'1,Ir'u.-J.,..~/J.I

-,I"".'~~

~l'rI1,os..OJp.I

l.,ok;'T~;,- ~~/I..,-,

/10~J.lI;'dv.-cVI/oa~

'~r"l-bl1lM~rx;ih-~~1 T

w-f~)/)L --

I

IBbC

I

IIIIIII

Chemical Sensory Experimental Protocol - 7/1/86

Set up: Organism in flow cell for 1 hour before starting test. Use IR Filters,keep doors closed.

Tr-eatmentsControls

- variDus preparations5ml of water from reservoir aquarium (the 10 gal. with pump)

Behavioral Experiment Protocol1. Select syringe

2. Mark syringe I, tape counter # on record sheet

3. Affix syringe

4. Start VCR - RECORD

5. Inject solution over 30 sec.

6. Remove syringe

7. Affix syringe with aquarium water

8. Inject aquarium water over 30 sec.

9. Continue recording for 5 min.

10. Stop recording

11. Run VCR 10 sec. on PLAY

12. Stop tape

13. Mark tape counter # on sheet

14. Wait 5 min. before beginning with next syringe.

1

--~--------------n-'-IIf!!!!I!! __---~r

-

~1

11

t9t1

-L---,.J

.L-L

_L-IL

...-"",,,--,,-=_.,

I'

11

J.

r--+

-----t-

---t-

-

IL

~.-:,.

--+-

~~

'----'114

'>Sl""-'-.

,

t/+

M0'

•...e

~_.

10-

l~~

tv"

~d

~I",

~~

~~

&<-

~..

~~

..•.•....·12

~r6

M<)

.••....•.•.

vI-~

..--+3 H-~+-+-+-.r-+-r-~-~~~~~~~-+-+-+-+~-III

H-~+-+-+-~+-~~~~~~~~~~-+-+-+-+-r-r~

+--- I

'-+-~---+--

sa