regenerative medicine anita mol, carlijn bouten, simon hoerstrup, frank baaijens laboratory for...
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Regenerative Medicine
Anita Mol, Carlijn Bouten, Simon Hoerstrup, Frank Baaijens
Laboratory for Tissue Biomechanics and Tissue Engineering, Department of Biomedical Engineering,
Eindhoven University of Technology
Healthcare-transforming technologies
ImagingEarlier diagnosis saves lives and
reduces costs
Minimally Invasive surgeryReducing patient trauma and reduces costs
Adapted from: Russ Coile, Futurescan 2003, SG-2
Clinical IT Right Information at the right time enables best treatment and reduces
costs
Molecular MedicinePreventing disease from happening and reduces costs
Regenerative medicineImplants taking over vital bodily functions, improving quality of life
Heart valves
Valve replacements
Artificial durability remarks
mechanical life-long trombogenic, noise
synthetic ? mechanical and hemodynamical behaviour ok
Biological
xenograft 7-10 yr Chemical fixation
allograft 7-10 yr Donor dependent
autograft > 15 yr Pulmonary valve transplant
300.000 replacements / year
Main drawback: no growth, repair and adaptation
Tissue Engineering Paradigm
Cells Scaffold(Mechanical)preconditioning
Tissue formation,matrix remodelling
Implantation/Model system
Implantation
Isolation of cells from vessels Seeding in scaffold Culture,
conditioningTissue formation
vsmc endothelial cells
Proof-of-concept: sheep cells
6 weeks
16 weeks
20 weeks
Hoerstrup et al. Circulation 2000
Not sufficient load-bearing properties to serve as aortic valve replacement
Implanted as pulmonary heart
valve replacement
Challenge
• Develop a living, autologous valve replacement, able to grow, repair and adapt to changing environment using human cells
• Sufficiently strong for aortic (high pressure) side
Strong, functional human valves!
Effective orifice area:1.52 0.21 cm2
Mean systolic gradient:11.5 3.1 mm Hg
Regurgitation:18.2 4.2 %
Dynamically conditioned tissue engineered human heart valve
Mol et al. Circulation 2006
Discussion
Tissue engineered human heart valves show promising features as aortic valve replacements
• Functional parameters are in the range of those reported for commonly used bioprostheses
• Upcoming animal studies will elucidate short- and long-term functionality of tissue-engineered heart valves in aortic position and the capability of growth and remodeling
Engineered tissues (In-vitro)
Acknowledgements
Eindhoven, BMT, TBMEDr. Anita MolDrs. Marjolein van LieshoutIr. Niels DriessenIr. Ralf BoerboomIr. Angelique BalguidIr. Rolf PullensIr. Martijn CoxIr. Mirjam RubbensChrista DamKaty KrahnDr. Carlijn BoutenDr. Marcel RuttenDr. Claudia VazDr. Gerrit PeetersProf. Bas de MolProf. Frank Baaijens
Eindhoven, BMT / ST, SMOIr. Eva WisseDrs. Patricia DankersDr. Nico SommerdijkDr. Maarten MerkxProf. Bert Meijer
Eindhoven, BMT, BEMIProf. Klaas Nicolaij
Dr. Gustav Strijkers
ZürichDr. Stephan NeuenschwanderDörthe Schmidt, MSc.Prof. Simon Hoerstrup
Leiden, TNO TPGDr. Ruud Bank
Rotterdam, Dutch Heart Valve BankDr. J. van Kats, prof. A. Bogers
GrantsBio-Initiative, Eindhoven University of Technology
Vici grant, Netherlands Organisation for Scientific Research
BioPolymers program, Dutch Polymer Institute