recombinant dna technology, forensic dna analysis and human genome project
TRANSCRIPT
HARAMAYA UNIVERSITY DNA Discovery 60th Anniversary Conference Theme: Discovery of DNA and its Impact
on Human Life
Recombinant DNA Technology, Forensic DNA Analysis and Human Genome Project.
By; Nateneal Tamerat June 07/2013
INTRODUCTION
Recombinant DNA technology: is series of procedure used to join together DNA fragments of different cells to produce new genetic composition that have value to human kind.
It is a technology that was engineered by Stanley Norman 1973.
But it was the discovery of restriction endonuclease , that leads to the development of recombinant DNA technology.
Restriction Endonuclease(RE)Is an enzyme that cuts DNA strands at specific recognition site.
For the first time RE HindII discovered in 1970 by Daniel Nathan and his colleagues.
Now a days there are more than 3000 RE used for DNA modification &manipulation.
How recombinant DNA created DNA isolation DNA sort out Incorporation in to vector
Transformation into the host Gene expression
Vectors
Bactriophage
Cosmid
plasmid
Application of DNA recombinant technology
Forensic analysis Agricultural
Organ transplant
Diagnosis of genetic disease Human Genome
Project
Forensic DNA Analysis Identifying victim and suspect by matching
genetic Samples with DNA database.The idea first raised by sir Alec Jeffreys in 1984.
For the first time forensic DNA analysis is used in the case of Collin pitchfork accusation for murder and sexual assault in British 1986.
Crime scene investigator create DNA profile by: Identify source of evidence from
Teeth
Sperm cell
Bones
blood
Hair follicle
some of DNA sample processing & techniques
Mitochondrial DNA analysisY- chromosome analysis
RFLP STR
Some Advantages of forensic DNA analysis
Crime investigation Wildlife conservation Parental test Organ transplantation Identification of pollution Authentication
HUMAN GENOME PROJECTA Project with an objectives of characterizing
human genetic material by determining the complete sequence of DNA in the human genome.
The program first launched by U.S. Department of Energy and the National Institutes of Health, Later on it was run by both public and private sectors of international consortium and Celera respectively, resulting the first draft human genome sequence in 2001.
Goal of HGP
Identify all the approximate 30,000 genes in human DNA.
Determine the sequences of the 3 billion chemical base pairs that make up human DNA.
Store this information in databases.
Improve tools for data analysis.
Transfer related technologies to the private sector.
Address the ethical, legal, and social issues that may arise from the project.
RESULTS OF HGP There is no scientific basis for racial distinction and
at the DNA level all people are 99.9 % alike.
The mutation speed is two times faster in men than in women.
paradigm change from one gene one protein to one gene multiple protein.
Fewer genes than expected, approx 31,000 Earlier estimates started at 100,000 genes.
More than 200 human genes are originally from bacteria these homologues don’t occur in fruit flies, roundworms or yeast.
comparative genomicsorganism Genome
bp
human 3500,000,000
Mouse 300,000,000
drosophila 170,000,000
round worm 110,000,000
Yeast 15,000,000
plant 100,000,000
Some Advantages of HGP
PharmacogenomicsGenetic predispositions to disease
Microbial BiofuelEvolution and Human Migration
Some Disadvantages of HGP
Confidentiality. Discrimination. Issue of patent. Widening the gap between the rich and poor.
CONCLUSION Recombinant DNA technology lends itself to theworld of medicine in a number of ways.
The most exiting application was mapping and sequencing of the human genome which is under taken by HGP.
This technology also involved in forensic science in investing criminal offence to find evidence that will indicate who the criminals are and bring justice.
THANK YOU