RECOMBINANT DNA TECHNOLOGY

Sulav Indra Paul

Deoxyribonucleic acid (DNA) is a long double-stranded chain of nucleotides

• DNA is made up of four nucleotides: A, C,

G, and T.

• A always pairs with T.

• C always pairs with G.

• The two strands of DNA are in an

antiparallel configuration.

Recombinent DNA:

Techniques in which DNA fragments are linked to self-replicating vectors to create recombinant DNA molecules which are replicated in a host cell.

Link: http://en.wikipedia.org/wiki/Recombinant_DNA

In 1953, the Double Helix DNA model was Discovered by “James Watson” & “Francis Crick”

Construction of recombinant DNA:

Cut with restriction enzymes

Isolate DNA

Ligate into cloning vector

Transform recombinant DNA molecule into host cell

Link: http://en.wikipedia.org/wiki/Recombinant_DNA

Recombinent DNA

process in plant:

Genetically Modified Crop(GM

Crop)

Recombinant DNA technology in Fish:

Link:www.google.com.bd/imgres?imgurl=http://ocw.mit.edu/courses/biological-engineering/20-020-introduction-to-biological-engineering-design-spring-2009/assignments/500px_GloFish.jpg&imgrefurl=http://ocw.mit.edu/courses/biological-engineering/20-020-introduction-to-biological-engineering-design-spring-2009/assignments/pdp8&usg=__1zaiCmy6aNzfcwc20Xcew5M1sJw=&h=333&w=500&sz=176&hl=en&start=1&zoom=1&tbnid=z1VtRgFIQa_j9M:&tbnh=87&tbnw=130&ei=S65-Ub6QH-OkiQKB3IGwCw&prev=/search%3Fq%3Dapplication%2Bof%2BrDNA%2Bin%2Bfish%26sa%3DG%26tbm%3Disch&itbs=1&sa=X&ved=0CAgQrQMwAA

Outcome of recombinant DNA technology• Recombinant DNA is widely used in biotechnology,

medicine and research.

• To produce GM crop(Genetically Modified Crop)

• Golden rice: a recombinant variety of rice that has been engineered to express the enzymes responsible for β-carotene biosynthesis.

• Herbicide-resistant crops.

• Insect-resistant crops

• Recombinant Chymosin: Found in Rennet, is an enzyme required to manufacture cheese.

Link: http://en.wikipedia.org/wiki/Recombinant_DNA

Some recombinant DNA products being used in human therapy

• INSULIN for diabetics • FACTOR VIII for males suffering from hemophilia A • FACTOR IX for hemophilia B • Human growth hormone (HGH) • Erythropoietin (EPO) for treating anemia • Several types of ‘Interferons’• Several ‘Interleukins’ • Granulocyte-macrophage colony-stimulating

factor (GM-CSF) for stimulating the bone marrow after a bone marrow transplant

• Parathyroid hormone

Link: http://en.wikipedia.org/wiki/Recombinant_DNA

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